Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303760 | sp|Q9LUV2|POP3_ARATH | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.56e-44 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303760 | A0A151TDN5 | 67.857 | 112 | 33 | 1 | 1 | 112 | 1 | 109 | 3.47e-48 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004340 | Msa1303760 | 0.842353 | 2.723978e-58 | -8.615850e-47 |
| Msa0012380 | Msa1303760 | 0.824838 | 6.496124e-54 | -8.615850e-47 |
| Msa0037270 | Msa1303760 | 0.804857 | 1.765975e-49 | -8.615850e-47 |
| Msa0072390 | Msa1303760 | 0.824519 | 7.722938e-54 | -8.615850e-47 |
| Msa0080810 | Msa1303760 | 0.829331 | 5.466281e-55 | -8.615850e-47 |
| Msa0081120 | Msa1303760 | 0.827948 | 1.179939e-54 | -8.615850e-47 |
| Msa0091470 | Msa1303760 | 0.804078 | 2.567177e-49 | -8.615850e-47 |
| Msa0116470 | Msa1303760 | 0.809355 | 1.971262e-50 | -8.615850e-47 |
| Msa0116510 | Msa1303760 | 0.825870 | 3.703347e-54 | -8.615850e-47 |
| Msa0120320 | Msa1303760 | 0.810772 | 9.762768e-51 | -8.615850e-47 |
| Msa0608780 | Msa1303760 | 0.823456 | 1.371455e-53 | -8.615850e-47 |
| Msa0610780 | Msa1303760 | 0.849623 | 2.872063e-60 | -8.615850e-47 |
| Msa0610810 | Msa1303760 | 0.831249 | 1.857503e-55 | -8.615850e-47 |
| Msa0615430 | Msa1303760 | 0.800513 | 1.391465e-48 | -8.615850e-47 |
| Msa0619390 | Msa1303760 | 0.808731 | 2.682038e-50 | -8.615850e-47 |
| Msa0627050 | Msa1303760 | 0.802967 | 4.362366e-49 | -8.615850e-47 |
| Msa0645410 | Msa1303760 | 0.811823 | 5.772607e-51 | -8.615850e-47 |
| Msa0646190 | Msa1303760 | 0.810840 | 9.436200e-51 | -8.615850e-47 |
| Msa0649250 | Msa1303760 | 0.821416 | 4.085355e-53 | -8.615850e-47 |
| Msa0651310 | Msa1303760 | 0.847204 | 1.341031e-59 | -8.615850e-47 |
| Msa0654880 | Msa1303760 | 0.802921 | 4.459819e-49 | -8.615850e-47 |
| Msa0693400 | Msa1303760 | 0.821348 | 4.234454e-53 | -8.615850e-47 |
| Msa0693500 | Msa1303760 | 0.833968 | 3.931095e-56 | -8.615850e-47 |
| Msa0698650 | Msa1303760 | 0.804148 | 2.482397e-49 | -8.615850e-47 |
| Msa0702560 | Msa1303760 | 0.805198 | 1.498418e-49 | -8.615850e-47 |
| Msa0705810 | Msa1303760 | 0.824194 | 9.209960e-54 | -8.615850e-47 |
| Msa0742090 | Msa1303760 | 0.817156 | 3.817158e-52 | -8.615850e-47 |
| Msa0749150 | Msa1303760 | 0.818437 | 1.961061e-52 | -8.615850e-47 |
| Msa0803530 | Msa1303760 | 0.859376 | 4.318283e-63 | -8.615850e-47 |
| Msa0823310 | Msa1303760 | 0.836774 | 7.682949e-57 | -8.615850e-47 |
| Msa0147540 | Msa1303760 | 0.809837 | 1.553332e-50 | -8.615850e-47 |
| Msa0163960 | Msa1303760 | 0.815272 | 1.006306e-51 | -8.615850e-47 |
| Msa0173370 | Msa1303760 | 0.819515 | 1.115139e-52 | -8.615850e-47 |
| Msa0180100 | Msa1303760 | 0.856644 | 2.798156e-62 | -8.615850e-47 |
| Msa0184510 | Msa1303760 | 0.869202 | 3.694578e-66 | -8.615850e-47 |
| Msa0187980 | Msa1303760 | 0.859631 | 3.618041e-63 | -8.615850e-47 |
| Msa0207680 | Msa1303760 | -0.804260 | 2.352183e-49 | -8.615850e-47 |
| Msa1228460 | Msa1303760 | 0.803729 | 3.033535e-49 | -8.615850e-47 |
| Msa1236860 | Msa1303760 | 0.873013 | 2.042941e-67 | -8.615850e-47 |
| Msa1237970 | Msa1303760 | 0.802229 | 6.193326e-49 | -8.615850e-47 |
| Msa1255020 | Msa1303760 | 0.865066 | 7.732231e-65 | -8.615850e-47 |
| Msa1295470 | Msa1303760 | 0.864926 | 8.552746e-65 | -8.615850e-47 |
| Msa1297220 | Msa1303760 | 0.826048 | 3.358669e-54 | -8.615850e-47 |
| Msa1303760 | Msa1303820 | 0.976656 | 3.037957e-142 | -8.615850e-47 |
| Msa1303760 | Msa1317240 | 0.862375 | 5.297745e-64 | -8.615850e-47 |
| Msa1303760 | Msa1323260 | 0.824261 | 8.883420e-54 | -8.615850e-47 |
| Msa1303760 | Msa1332690 | 0.803360 | 3.618344e-49 | -8.615850e-47 |
| Msa1303760 | Msa1343450 | 0.835613 | 1.515354e-56 | -8.615850e-47 |
| Msa1303760 | Msa1359890 | 0.806436 | 8.235418e-50 | -8.615850e-47 |
| Msa1303760 | Msa1363070 | 0.971970 | 5.224374e-134 | -8.615850e-47 |
| Msa1303760 | Msa1369180 | 0.814603 | 1.416352e-51 | -8.615850e-47 |
| Msa1303760 | Msa1373390 | 0.805225 | 1.479581e-49 | -8.615850e-47 |
| Msa1303760 | Msa1382750 | 0.823232 | 1.547791e-53 | -8.615850e-47 |
| Msa1303760 | Msa1404690 | 0.813547 | 2.421665e-51 | -8.615850e-47 |
| Msa1303760 | Msa1450780 | 0.840909 | 6.545191e-58 | -8.615850e-47 |
| Msa1303760 | Msa1456620 | 0.814379 | 1.587482e-51 | -8.615850e-47 |
| Msa1303760 | Msa1460530 | 0.806512 | 7.935671e-50 | -8.615850e-47 |
| Msa0378310 | Msa1303760 | 0.813253 | 2.810537e-51 | -8.615850e-47 |
| Msa0404060 | Msa1303760 | 0.844171 | 8.920271e-59 | -8.615850e-47 |
| Msa0404070 | Msa1303760 | 0.832801 | 7.683709e-56 | -8.615850e-47 |
| Msa0425320 | Msa1303760 | 0.828977 | 6.660302e-55 | -8.615850e-47 |
| Msa0428330 | Msa1303760 | 0.802705 | 4.942176e-49 | -8.615850e-47 |
| Msa0468360 | Msa1303760 | 0.819888 | 9.166459e-53 | -8.615850e-47 |
| Msa0471760 | Msa1303760 | 0.812530 | 4.047773e-51 | -8.615850e-47 |
| Msa0477030 | Msa1303760 | -0.802456 | 5.563680e-49 | -8.615850e-47 |
| Msa1014700 | Msa1303760 | 0.803863 | 2.845904e-49 | -8.615850e-47 |
| Msa1031530 | Msa1303760 | 0.805715 | 1.167887e-49 | -8.615850e-47 |
| Msa1032740 | Msa1303760 | -0.801889 | 7.277644e-49 | -8.615850e-47 |
| Msa1071640 | Msa1303760 | 0.825559 | 4.388583e-54 | -8.615850e-47 |
| Msa1078600 | Msa1303760 | 0.810551 | 1.089831e-50 | -8.615850e-47 |
| Msa1078770 | Msa1303760 | 0.804396 | 2.204273e-49 | -8.615850e-47 |
| Msa0238860 | Msa1303760 | 0.804293 | 2.315590e-49 | -8.615850e-47 |
| Msa0240590 | Msa1303760 | 0.800807 | 1.211923e-48 | -8.615850e-47 |
| Msa0316610 | Msa1303760 | 0.806689 | 7.282099e-50 | -8.615850e-47 |
| Msa1117510 | Msa1303760 | 0.833388 | 5.487950e-56 | -8.615850e-47 |
| Msa1158450 | Msa1303760 | 0.800983 | 1.115598e-48 | -8.615850e-47 |
| Msa1161210 | Msa1303760 | 0.820246 | 7.592410e-53 | -8.615850e-47 |
| Msa1173380 | Msa1303760 | 0.817807 | 2.722736e-52 | -8.615850e-47 |
| Msa1209160 | Msa1303760 | 0.975525 | 4.100829e-140 | -8.615850e-47 |
| Msa1219060 | Msa1303760 | 0.812025 | 5.217956e-51 | -8.615850e-47 |
| Msa0508780 | Msa1303760 | 0.809076 | 2.262085e-50 | -8.615850e-47 |
| Msa0515460 | Msa1303760 | 0.816065 | 6.699441e-52 | -8.615850e-47 |
| Msa0530340 | Msa1303760 | 0.815710 | 8.039357e-52 | -8.615850e-47 |
| Msa0549110 | Msa1303760 | 0.800584 | 1.345823e-48 | -8.615850e-47 |
| Msa0556070 | Msa1303760 | 0.841044 | 6.031430e-58 | -8.615850e-47 |
| Msa0570680 | Msa1303760 | 0.836022 | 1.193608e-56 | -8.615850e-47 |
| Msa0576180 | Msa1303760 | -0.801801 | 7.584954e-49 | -8.615850e-47 |
| Msa0577050 | Msa1303760 | 0.832768 | 7.827863e-56 | -8.615850e-47 |
| Msa0579230 | Msa1303760 | 0.807915 | 4.003597e-50 | -8.615850e-47 |
| Msa0587250 | Msa1303760 | 0.807229 | 5.597415e-50 | -8.615850e-47 |
| Msa0864730 | Msa1303760 | 0.800440 | 1.439695e-48 | -8.615850e-47 |
| Msa0864750 | Msa1303760 | 0.802009 | 6.874753e-49 | -8.615850e-47 |
| Msa0879650 | Msa1303760 | 0.858962 | 5.745610e-63 | -8.615850e-47 |
| Msa0887100 | Msa1303760 | 0.886188 | 4.250509e-72 | -8.615850e-47 |
| Msa0892020 | Msa1303760 | 0.830821 | 2.366389e-55 | -8.615850e-47 |
| Msa0909720 | Msa1303760 | 0.817530 | 3.144626e-52 | -8.615850e-47 |
| Msa0913140 | Msa1303760 | 0.874312 | 7.453292e-68 | -8.615850e-47 |
| Msa0925630 | Msa1303760 | 0.803827 | 2.894479e-49 | -8.615850e-47 |
| Msa0928940 | Msa1303760 | 0.808888 | 2.482138e-50 | -8.615850e-47 |
| Msa0930210 | Msa1303760 | -0.811117 | 8.217958e-51 | -8.615850e-47 |
| Msa0950470 | Msa1303760 | 0.852488 | 4.467790e-61 | -8.615850e-47 |
| Msa0950570 | Msa1303760 | 0.860848 | 1.551205e-63 | -8.615850e-47 |
| Msa0956910 | Msa1303760 | 0.846394 | 2.233107e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303760 | MtrunA17_Chr4g0070211 | 75.000 | 72 | 15 | 1 | 8 | 79 | 3 | 71 | 2.03e-30 | 103 |
| Msa1303760 | MtrunA17_Chr2g0321051 | 36.893 | 103 | 60 | 2 | 9 | 111 | 8 | 105 | 1.39e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303760 | AT3G17210.1 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.60e-45 | 142 |
| Msa1303760 | AT3G17210.2 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.60e-45 | 142 |
| Msa1303760 | AT5G22580.1 | 34.021 | 97 | 59 | 2 | 9 | 105 | 7 | 98 | 8.53e-15 | 65.5 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTCCTCAATCTTCTCT+TGG | 0.329948 | 8_3:+67025381 | None:intergenic |
| CAAAGTGAAAGAGGAGGAAA+TGG | 0.338863 | 8_3:-67025462 | Msa1303760:exon |
| GATTCAGGTAACAAATAATA+AGG | 0.348275 | 8_3:+67025063 | None:intergenic |
| AATGAGTAAATCCTTGATTC+AGG | 0.352373 | 8_3:+67025048 | None:intergenic |
| AGGAGGGAGCAAAGTGAAAG+AGG | 0.406040 | 8_3:-67025471 | Msa1303760:five_prime_UTR |
| TTACCAATGGAATGACTTCA+TGG | 0.420824 | 8_3:+67025322 | None:intergenic |
| ATTATTTGTTACCTGAATCA+AGG | 0.432767 | 8_3:-67025059 | Msa1303760:CDS |
| AAAGTTGCTCGTTCCTTGCT+TGG | 0.439365 | 8_3:-67024952 | Msa1303760:CDS |
| GAGTTCCTCAATCTTCTCTT+GGG | 0.443255 | 8_3:+67025382 | None:intergenic |
| GTAGTGAAGAACATAGTGAT+TGG | 0.457715 | 8_3:-67025425 | Msa1303760:CDS |
| AGTGAAAGAGGAGGAAATGG+AGG | 0.502250 | 8_3:-67025459 | Msa1303760:exon |
| GAGGAAATGGAGGAAGCAAA+AGG | 0.502851 | 8_3:-67025449 | Msa1303760:exon |
| TGGGATGAGATTGACGAGAT+TGG | 0.516286 | 8_3:+67025346 | None:intergenic |
| ATTCAAAGGTTAACGATGGT+GGG | 0.545431 | 8_3:+67024903 | None:intergenic |
| TCCTTGCTTGGAGAAAGTGC+TGG | 0.548785 | 8_3:-67024940 | Msa1303760:CDS |
| CACCCATGAAGTCATTCCAT+TGG | 0.553232 | 8_3:-67025325 | Msa1303760:intron |
| TGAATCAACATTTGAGAGTG+TGG | 0.555452 | 8_3:-67025021 | Msa1303760:CDS |
| TTTGCATACTCAACATGAGC+AGG | 0.558557 | 8_3:+67024972 | None:intergenic |
| GAAGATTGAGGAACTCATCA+AGG | 0.571882 | 8_3:-67025375 | Msa1303760:CDS |
| GCCAGCACTTTCTCCAAGCA+AGG | 0.576667 | 8_3:+67024939 | None:intergenic |
| GATTCAAAGGTTAACGATGG+TGG | 0.578082 | 8_3:+67024902 | None:intergenic |
| GAGATTGACGAGATTGGCAA+AGG | 0.580515 | 8_3:+67025352 | None:intergenic |
| AGGGAGCAAAGTGAAAGAGG+AGG | 0.582719 | 8_3:-67025468 | Msa1303760:five_prime_UTR |
| CAATGGAATGACTTCATGGG+TGG | 0.583790 | 8_3:+67025326 | None:intergenic |
| AATCTCCCAAGAGAAGATTG+AGG | 0.597731 | 8_3:-67025387 | Msa1303760:CDS |
| ATGAGCTACATACTCTGCAA+TGG | 0.614733 | 8_3:+67024995 | None:intergenic |
| TACCAATGGAATGACTTCAT+GGG | 0.631729 | 8_3:+67025323 | None:intergenic |
| AAGAAACACTGCAATGCACG+AGG | 0.694375 | 8_3:-67025522 | None:intergenic |
| AATGGAATGACTTCATGGGT+GGG | 0.706119 | 8_3:+67025327 | None:intergenic |
| ACGGATTCAAAGGTTAACGA+TGG | 0.711712 | 8_3:+67024899 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTTTTTTAAGTTT+TGG | + | chr8_3:67025199-67025218 | None:intergenic | 10.0% |
| !!! | TATACATGTCTTATTTTTTT+AGG | - | chr8_3:67025316-67025335 | Msa1303760:intron | 15.0% |
| !!! | TTGTAGTATACAGCTATTTT+AGG | - | chr8_3:67024928-67024947 | Msa1303760:CDS | 25.0% |
| !! | TTTAAGTTTCATCTTACCAA+TGG | + | chr8_3:67025113-67025132 | None:intergenic | 25.0% |
| ! | GATTCAGGTAACAAATAATA+AGG | + | chr8_3:67025359-67025378 | None:intergenic | 25.0% |
| !! | ATTATTTGTTACCTGAATCA+AGG | - | chr8_3:67025360-67025379 | Msa1303760:CDS | 25.0% |
| !! | TAGTATACAGCTATTTTAGG+AGG | - | chr8_3:67024931-67024950 | Msa1303760:CDS | 30.0% |
| !! | AGTATACAGCTATTTTAGGA+GGG | - | chr8_3:67024932-67024951 | Msa1303760:CDS | 30.0% |
| AATGAGTAAATCCTTGATTC+AGG | + | chr8_3:67025374-67025393 | None:intergenic | 30.0% | |
| GTAGTGAAGAACATAGTGAT+TGG | - | chr8_3:67024994-67025013 | Msa1303760:CDS | 35.0% | |
| TACCAATGGAATGACTTCAT+GGG | + | chr8_3:67025099-67025118 | None:intergenic | 35.0% | |
| TTACCAATGGAATGACTTCA+TGG | + | chr8_3:67025100-67025119 | None:intergenic | 35.0% | |
| !! | ATAGTCTAGACTGCTTTTGT+CGG | + | chr8_3:67025149-67025168 | None:intergenic | 35.0% |
| TGAATCAACATTTGAGAGTG+TGG | - | chr8_3:67025398-67025417 | Msa1303760:CDS | 35.0% | |
| CAAAGTGAAAGAGGAGGAAA+TGG | - | chr8_3:67024957-67024976 | Msa1303760:CDS | 40.0% | |
| AATCTCCCAAGAGAAGATTG+AGG | - | chr8_3:67025032-67025051 | Msa1303760:CDS | 40.0% | |
| GAGTTCCTCAATCTTCTCTT+GGG | + | chr8_3:67025040-67025059 | None:intergenic | 40.0% | |
| TGAGTTCCTCAATCTTCTCT+TGG | + | chr8_3:67025041-67025060 | None:intergenic | 40.0% | |
| GAAGATTGAGGAACTCATCA+AGG | - | chr8_3:67025044-67025063 | Msa1303760:CDS | 40.0% | |
| AATGGAATGACTTCATGGGT+GGG | + | chr8_3:67025095-67025114 | None:intergenic | 40.0% | |
| ATGAGCTACATACTCTGCAA+TGG | + | chr8_3:67025427-67025446 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGAGC+AGG | + | chr8_3:67025450-67025469 | None:intergenic | 40.0% | |
| AGTGAAAGAGGAGGAAATGG+AGG | - | chr8_3:67024960-67024979 | Msa1303760:CDS | 45.0% | |
| GAGGAAATGGAGGAAGCAAA+AGG | - | chr8_3:67024970-67024989 | Msa1303760:CDS | 45.0% | |
| GAGATTGACGAGATTGGCAA+AGG | + | chr8_3:67025070-67025089 | None:intergenic | 45.0% | |
| TGGGATGAGATTGACGAGAT+TGG | + | chr8_3:67025076-67025095 | None:intergenic | 45.0% | |
| CAATGGAATGACTTCATGGG+TGG | + | chr8_3:67025096-67025115 | None:intergenic | 45.0% | |
| CACCCATGAAGTCATTCCAT+TGG | - | chr8_3:67025094-67025113 | Msa1303760:intron | 45.0% | |
| ! | GATTTTCGCTAAGTGGACGA+GGG | + | chr8_3:67025235-67025254 | None:intergenic | 45.0% |
| ! | TACCAGCGATTTTCGCTAAG+TGG | + | chr8_3:67025242-67025261 | None:intergenic | 45.0% |
| AAAGTTGCTCGTTCCTTGCT+TGG | - | chr8_3:67025467-67025486 | Msa1303760:five_prime_UTR | 45.0% | |
| AGGAGGGAGCAAAGTGAAAG+AGG | - | chr8_3:67024948-67024967 | Msa1303760:CDS | 50.0% | |
| AGGGAGCAAAGTGAAAGAGG+AGG | - | chr8_3:67024951-67024970 | Msa1303760:CDS | 50.0% | |
| ! | CGATTTTCGCTAAGTGGACG+AGG | + | chr8_3:67025236-67025255 | None:intergenic | 50.0% |
| GTCCACTTAGCGAAAATCGC+TGG | - | chr8_3:67025237-67025256 | Msa1303760:intron | 50.0% | |
| ! | TCCTTGCTTGGAGAAAGTGC+TGG | - | chr8_3:67025479-67025498 | Msa1303760:five_prime_UTR | 50.0% |
| GCCAGCACTTTCTCCAAGCA+AGG | + | chr8_3:67025483-67025502 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 67024905 | 67025536 | 67024905 | ID=Msa1303760;Name=Msa1303760 |
| chr8_3 | mRNA | 67024905 | 67025536 | 67024905 | ID=Msa1303760-mRNA-1;Parent=Msa1303760;Name=Msa1303760-mRNA-1;_AED=0.44;_eAED=0.45;_QI=71|0|0.5|1|1|1|2|0|112 |
| chr8_3 | exon | 67024905 | 67025103 | 67024905 | ID=Msa1303760-mRNA-1:exon:20484;Parent=Msa1303760-mRNA-1 |
| chr8_3 | exon | 67025326 | 67025536 | 67025326 | ID=Msa1303760-mRNA-1:exon:20483;Parent=Msa1303760-mRNA-1 |
| chr8_3 | five_prime_UTR | 67025466 | 67025536 | 67025466 | ID=Msa1303760-mRNA-1:five_prime_utr;Parent=Msa1303760-mRNA-1 |
| chr8_3 | CDS | 67025326 | 67025465 | 67025326 | ID=Msa1303760-mRNA-1:cds;Parent=Msa1303760-mRNA-1 |
| chr8_3 | CDS | 67024905 | 67025103 | 67024905 | ID=Msa1303760-mRNA-1:cds;Parent=Msa1303760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
