AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1209160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1209160 MtrunA17_Chr4g0070211 75.000 72 15 1 8 79 3 71 2.03e-30 103
Msa1209160 MtrunA17_Chr2g0321051 36.893 103 60 2 9 111 8 105 1.39e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1209160 AT3G17210.1 62.500 112 39 1 1 112 1 109 2.60e-45 142
Msa1209160 AT3G17210.2 62.500 112 39 1 1 112 1 109 2.60e-45 142
Msa1209160 AT5G22580.1 34.021 97 59 2 9 105 7 98 8.53e-15 65.5

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTCCTCAATCTTCTCT+TGG 0.329948 8_1:+67584466 None:intergenic
CAAAGTGAAAGAGGAGGAAA+TGG 0.338863 8_1:-67584547 Msa1209160:exon
GATTCAGGTAACAAATAATA+AGG 0.348275 8_1:+67584148 None:intergenic
AATGAGTAAATCCTTGATTC+AGG 0.352373 8_1:+67584133 None:intergenic
AGGAGGGAGCAAAGTGAAAG+AGG 0.406040 8_1:-67584556 Msa1209160:five_prime_UTR
TTACCAATGGAATGACTTCA+TGG 0.420824 8_1:+67584407 None:intergenic
ATTATTTGTTACCTGAATCA+AGG 0.432767 8_1:-67584144 Msa1209160:CDS
AAAGTTGCTCGTTCCTTGCT+TGG 0.439365 8_1:-67584037 Msa1209160:CDS
GAGTTCCTCAATCTTCTCTT+GGG 0.443255 8_1:+67584467 None:intergenic
GTAGTGAAGAACATAGTGAT+TGG 0.457715 8_1:-67584510 Msa1209160:CDS
AGTGAAAGAGGAGGAAATGG+AGG 0.502250 8_1:-67584544 Msa1209160:exon
GAGGAAATGGAGGAAGCAAA+AGG 0.502851 8_1:-67584534 Msa1209160:exon
TGGGATGAGATTGACGAGAT+TGG 0.516286 8_1:+67584431 None:intergenic
ATTCAAAGGTTAACGATGGT+GGG 0.545431 8_1:+67583988 None:intergenic
TCCTTGCTTGGAGAAAGTGC+TGG 0.548785 8_1:-67584025 Msa1209160:CDS
CACCCATGAAGTCATTCCAT+TGG 0.553232 8_1:-67584410 Msa1209160:intron
TGAATCAACATTTGAGAGTG+TGG 0.555452 8_1:-67584106 Msa1209160:CDS
TTTGCATACTCAACATGAGC+AGG 0.558557 8_1:+67584057 None:intergenic
GAAGATTGAGGAACTCATCA+AGG 0.571882 8_1:-67584460 Msa1209160:CDS
GCCAGCACTTTCTCCAAGCA+AGG 0.576667 8_1:+67584024 None:intergenic
GATTCAAAGGTTAACGATGG+TGG 0.578082 8_1:+67583987 None:intergenic
GAGATTGACGAGATTGGCAA+AGG 0.580515 8_1:+67584437 None:intergenic
AGGGAGCAAAGTGAAAGAGG+AGG 0.582719 8_1:-67584553 Msa1209160:five_prime_UTR
CAATGGAATGACTTCATGGG+TGG 0.583790 8_1:+67584411 None:intergenic
AATCTCCCAAGAGAAGATTG+AGG 0.597731 8_1:-67584472 Msa1209160:CDS
ATGAGCTACATACTCTGCAA+TGG 0.614733 8_1:+67584080 None:intergenic
TACCAATGGAATGACTTCAT+GGG 0.631729 8_1:+67584408 None:intergenic
AAGAAACACTGCAATGCACG+AGG 0.694375 8_1:-67584607 None:intergenic
AATGGAATGACTTCATGGGT+GGG 0.706119 8_1:+67584412 None:intergenic
ACGGATTCAAAGGTTAACGA+TGG 0.711712 8_1:+67583984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAAGTTTTTTAAGTTT+TGG + chr8_1:67584284-67584303 None:intergenic 10.0%
!!! TATACATGTCTTATTTTTTT+AGG - chr8_1:67584401-67584420 Msa1209160:intron 15.0%
!!! TTGTAGTATACAGCTATTTT+AGG - chr8_1:67584013-67584032 Msa1209160:CDS 25.0%
!! TTTAAGTTTCATCTTACCAA+TGG + chr8_1:67584198-67584217 None:intergenic 25.0%
! GATTCAGGTAACAAATAATA+AGG + chr8_1:67584444-67584463 None:intergenic 25.0%
!! ATTATTTGTTACCTGAATCA+AGG - chr8_1:67584445-67584464 Msa1209160:CDS 25.0%
!! TAGTATACAGCTATTTTAGG+AGG - chr8_1:67584016-67584035 Msa1209160:CDS 30.0%
!! AGTATACAGCTATTTTAGGA+GGG - chr8_1:67584017-67584036 Msa1209160:CDS 30.0%
AATGAGTAAATCCTTGATTC+AGG + chr8_1:67584459-67584478 None:intergenic 30.0%
GTAGTGAAGAACATAGTGAT+TGG - chr8_1:67584079-67584098 Msa1209160:CDS 35.0%
TACCAATGGAATGACTTCAT+GGG + chr8_1:67584184-67584203 None:intergenic 35.0%
TTACCAATGGAATGACTTCA+TGG + chr8_1:67584185-67584204 None:intergenic 35.0%
!! ATAGTCTAGACTGCTTTTGT+CGG + chr8_1:67584234-67584253 None:intergenic 35.0%
TGAATCAACATTTGAGAGTG+TGG - chr8_1:67584483-67584502 Msa1209160:CDS 35.0%
CAAAGTGAAAGAGGAGGAAA+TGG - chr8_1:67584042-67584061 Msa1209160:CDS 40.0%
AATCTCCCAAGAGAAGATTG+AGG - chr8_1:67584117-67584136 Msa1209160:CDS 40.0%
GAGTTCCTCAATCTTCTCTT+GGG + chr8_1:67584125-67584144 None:intergenic 40.0%
TGAGTTCCTCAATCTTCTCT+TGG + chr8_1:67584126-67584145 None:intergenic 40.0%
GAAGATTGAGGAACTCATCA+AGG - chr8_1:67584129-67584148 Msa1209160:CDS 40.0%
AATGGAATGACTTCATGGGT+GGG + chr8_1:67584180-67584199 None:intergenic 40.0%
ATGAGCTACATACTCTGCAA+TGG + chr8_1:67584512-67584531 None:intergenic 40.0%
TTTGCATACTCAACATGAGC+AGG + chr8_1:67584535-67584554 None:intergenic 40.0%
AGTGAAAGAGGAGGAAATGG+AGG - chr8_1:67584045-67584064 Msa1209160:CDS 45.0%
GAGGAAATGGAGGAAGCAAA+AGG - chr8_1:67584055-67584074 Msa1209160:CDS 45.0%
GAGATTGACGAGATTGGCAA+AGG + chr8_1:67584155-67584174 None:intergenic 45.0%
TGGGATGAGATTGACGAGAT+TGG + chr8_1:67584161-67584180 None:intergenic 45.0%
CAATGGAATGACTTCATGGG+TGG + chr8_1:67584181-67584200 None:intergenic 45.0%
CACCCATGAAGTCATTCCAT+TGG - chr8_1:67584179-67584198 Msa1209160:intron 45.0%
! GATTTTCGCTAAGTGGACGA+GGG + chr8_1:67584320-67584339 None:intergenic 45.0%
! TACCAGCGATTTTCGCTAAG+TGG + chr8_1:67584327-67584346 None:intergenic 45.0%
AAAGTTGCTCGTTCCTTGCT+TGG - chr8_1:67584552-67584571 Msa1209160:five_prime_UTR 45.0%
AGGAGGGAGCAAAGTGAAAG+AGG - chr8_1:67584033-67584052 Msa1209160:CDS 50.0%
AGGGAGCAAAGTGAAAGAGG+AGG - chr8_1:67584036-67584055 Msa1209160:CDS 50.0%
! CGATTTTCGCTAAGTGGACG+AGG + chr8_1:67584321-67584340 None:intergenic 50.0%
GTCCACTTAGCGAAAATCGC+TGG - chr8_1:67584322-67584341 Msa1209160:intron 50.0%
! TCCTTGCTTGGAGAAAGTGC+TGG - chr8_1:67584564-67584583 Msa1209160:five_prime_UTR 50.0%
GCCAGCACTTTCTCCAAGCA+AGG + chr8_1:67584568-67584587 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 67583990 67584621 67583990 ID=Msa1209160;Name=Msa1209160
chr8_1 mRNA 67583990 67584621 67583990 ID=Msa1209160-mRNA-1;Parent=Msa1209160;Name=Msa1209160-mRNA-1;_AED=0.36;_eAED=0.37;_QI=71|0|0.5|1|1|1|2|0|112
chr8_1 exon 67583990 67584188 67583990 ID=Msa1209160-mRNA-1:exon:22362;Parent=Msa1209160-mRNA-1
chr8_1 exon 67584411 67584621 67584411 ID=Msa1209160-mRNA-1:exon:22361;Parent=Msa1209160-mRNA-1
chr8_1 five_prime_UTR 67584551 67584621 67584551 ID=Msa1209160-mRNA-1:five_prime_utr;Parent=Msa1209160-mRNA-1
chr8_1 CDS 67584411 67584550 67584411 ID=Msa1209160-mRNA-1:cds;Parent=Msa1209160-mRNA-1
chr8_1 CDS 67583990 67584188 67583990 ID=Msa1209160-mRNA-1:cds;Parent=Msa1209160-mRNA-1
Gene Sequence

>Msa1209160

ATGGAGGAAGCAAAAGGAGTAGTGAAGAACATAGTGATTGGAAAGTTCAAAGATGAAATCTCCCAAGAGAAGATTGAGGAACTCATCAAGGCCTTTGCCAATCTCGTCAATCTCATCCCACCCATGAAGTCATTCCATTGGAATTGTTGTTATACTTTCCTTATTATTTGTTACCTGAATCAAGGATTTACTCATTTCTTTGAATCAACATTTGAGAGTGTGGAAGCCATTGCAGAGTATGTAGCTCATCCTGCTCATGTTGAGTATGCAAAGTTGCTCGTTCCTTGCTTGGAGAAAGTGCTGGCATTTGATTATAAGCCCACCATCGTTAACCTTTGA

Protein sequence

>Msa1209160

MEEAKGVVKNIVIGKFKDEISQEKIEELIKAFANLVNLIPPMKSFHWNCCYTFLIICYLNQGFTHFFESTFESVEAIAEYVAHPAHVEYAKLLVPCLEKVLAFDYKPTIVNL*