AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0049390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0049390 MtrunA17_Chr7g0237101 87.952 83 10 0 1 83 1 83 6.19e-51 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0049390 AT3G13677.2 63.333 60 22 0 38 97 17 76 3.27e-21 80.1
Msa0049390 AT3G13677.3 63.333 60 22 0 38 97 17 76 3.27e-21 80.1
Msa0049390 AT3G13677.1 54.167 48 22 0 38 85 17 64 4.24e-12 59.3

Find 35 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTTGGTTGGAACAATTT+TGG 0.158239 1_2:+2680851 Msa0049390:five_prime_UTR
TTTAAAGTCATGCCCATATT+TGG 0.207257 1_2:-2684026 None:intergenic
ATGACACAGCGTAATGATTT+TGG 0.209604 1_2:-2680930 None:intergenic
CGGCACATCAAAGCCAAATA+TGG 0.223112 1_2:+2684013 Msa0049390:three_prime_UTR
TGAAAATGCTAATAAGTTAT+AGG 0.225964 1_2:-2684144 None:intergenic
CAGTAGTACAAGAAGAAATA+AGG 0.333259 1_2:+2683483 Msa0049390:CDS
TACTACTGTTTAAATTATCA+AGG 0.382671 1_2:-2683379 None:intergenic
TCTGCAACTTATGTCAGTTC+AGG 0.388016 1_2:+2683571 Msa0049390:CDS
GGCTATCTATGATGTATATT+AGG 0.389925 1_2:+2683909 Msa0049390:three_prime_UTR
TGATTGATTATAACTTATAT+AGG 0.409831 1_2:+2683888 Msa0049390:three_prime_UTR
ATCATAAATATCATATCATT+AGG 0.420417 1_2:-2684075 None:intergenic
AGAATTATCAACAAGCATGC+TGG 0.441552 1_2:+2683846 Msa0049390:three_prime_UTR
CATCATCCATCATTTGATCA+AGG 0.473735 1_2:-2683406 None:intergenic
AGCCGCGCAGGCATGCTTGA+AGG 0.475080 1_2:+2683935 Msa0049390:three_prime_UTR
ATCAATTGCTCGAGCAGGAA+TGG 0.481401 1_2:-2680876 None:intergenic
ATTTCAGAGAACTATATCTC+AGG 0.485584 1_2:+2683781 Msa0049390:intron
TCCTGATCAATTGCTCGAGC+AGG 0.499019 1_2:-2680881 None:intergenic
TCCTGCTCGAGCAATTGATC+AGG 0.499795 1_2:+2680880 Msa0049390:five_prime_UTR
GAGAACTATATCTCAGGAGC+AGG 0.501811 1_2:+2683787 Msa0049390:intron
TATATTAGGCGCAGCCGCGC+AGG 0.507348 1_2:+2683923 Msa0049390:three_prime_UTR
GGCACATCAAAGCCAAATAT+GGG 0.507858 1_2:+2684014 Msa0049390:three_prime_UTR
TATGGGCATGACTTTAAAAG+TGG 0.508332 1_2:+2684031 Msa0049390:three_prime_UTR
TTGATAGTGCAGTGCAAAAG+TGG 0.518366 1_2:+2683352 Msa0049390:intron
GAATTATCAACAAGCATGCT+GGG 0.518576 1_2:+2683847 Msa0049390:three_prime_UTR
ACTACTGTTTAAATTATCAA+GGG 0.518963 1_2:-2683378 None:intergenic
CTTGAGACGATGTGCGACAT+AGG 0.522340 1_2:-2683534 None:intergenic
TATATCTCAGGAGCAGGAGT+TGG 0.527070 1_2:+2683793 Msa0049390:CDS
TAATACCCTTGATCAAATGA+TGG 0.534465 1_2:+2683400 Msa0049390:CDS
AGTATGGTGTGAGAGAAAAC+AGG 0.549837 1_2:+2683460 Msa0049390:CDS
AAAATCATTACGCTGTGTCA+TGG 0.581847 1_2:+2680932 Msa0049390:exon
ATCATCCATCATTTGATCAA+GGG 0.590538 1_2:-2683405 None:intergenic
CTATGTCGCACATCGTCTCA+AGG 0.593279 1_2:+2683535 Msa0049390:CDS
TTGGTGAGTTGTGGAAAACA+CGG 0.618922 1_2:-2680911 None:intergenic
AACCTTCAAGCATGCCTGCG+CGG 0.670325 1_2:-2683937 None:intergenic
GAGTTGTGGAAAACACGGCG+AGG 0.735021 1_2:-2680906 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAACTATAATAATTGTT+TGG + chr1_2:2683162-2683181 Msa0049390:intron 10.0%
!!! ATTTTTGAACTTAAACAAAA+CGG - chr1_2:2681332-2681351 None:intergenic 15.0%
!!! CTCTTTTCATTAATAATATA+CGG + chr1_2:2682464-2682483 Msa0049390:intron 15.0%
!! AGAAGATAAATAAATTGTTT+AGG - chr1_2:2683006-2683025 None:intergenic 15.0%
!!! TATGAAACATAACAAAATTT+TGG + chr1_2:2683133-2683152 Msa0049390:intron 15.0%
!!! ACATTATCTTTTTTTTTTTG+AGG - chr1_2:2683692-2683711 None:intergenic 15.0%
!! TGATTGATTATAACTTATAT+AGG + chr1_2:2683888-2683907 Msa0049390:three_prime_UTR 15.0%
!!! TATAAAATGTTTTACACTAT+CGG + chr1_2:2683993-2684012 Msa0049390:three_prime_UTR 15.0%
!! ATCATAAATATCATATCATT+AGG - chr1_2:2684078-2684097 None:intergenic 15.0%
!! CTATTATTATAAACATGTTG+AGG - chr1_2:2681028-2681047 None:intergenic 20.0%
!!! GCAAATAAATAAGCTTTTTA+TGG - chr1_2:2681304-2681323 None:intergenic 20.0%
!!! TTTAGATTTAGCTTTAATAG+AGG + chr1_2:2681591-2681610 Msa0049390:intron 20.0%
!!! TATTTTGTCTTTTGATAAGT+TGG + chr1_2:2681628-2681647 Msa0049390:intron 20.0%
!!! TTGTTTTCGGTATATATTTT+AGG + chr1_2:2682080-2682099 Msa0049390:intron 20.0%
!!! TGTTTTCGGTATATATTTTA+GGG + chr1_2:2682081-2682100 Msa0049390:intron 20.0%
!! AATGTGCATATGATTATAAA+GGG - chr1_2:2682551-2682570 None:intergenic 20.0%
!! AAAAATAAAGAAATTTCGTC+TGG - chr1_2:2682729-2682748 None:intergenic 20.0%
!!! GACGAAATTTCTTTATTTTT+AGG + chr1_2:2682729-2682748 Msa0049390:intron 20.0%
!! ATTGTTCAGTAAAATTATTG+TGG - chr1_2:2683074-2683093 None:intergenic 20.0%
!! ACTACTGTTTAAATTATCAA+GGG - chr1_2:2683381-2683400 None:intergenic 20.0%
!! TACTACTGTTTAAATTATCA+AGG - chr1_2:2683382-2683401 None:intergenic 20.0%
!!! TATAGTTCTCTGAAATAAAA+GGG - chr1_2:2683777-2683796 None:intergenic 20.0%
!!! ATATAGTTCTCTGAAATAAA+AGG - chr1_2:2683778-2683797 None:intergenic 20.0%
!!! GTTTAATTTTCATAAACTGT+TGG + chr1_2:2683970-2683989 Msa0049390:three_prime_UTR 20.0%
!! TGAAAATGCTAATAAGTTAT+AGG - chr1_2:2684147-2684166 None:intergenic 20.0%
! AAAATAAACCAATGATTGAG+AGG - chr1_2:2680964-2680983 None:intergenic 25.0%
!!! TTGTTCTAAGTTTTGAAATC+TGG + chr1_2:2681075-2681094 Msa0049390:intron 25.0%
! AGCTTATTTATAACCATTCA+CGG - chr1_2:2681373-2681392 None:intergenic 25.0%
! ATCTAAATGCCTAATAGTAA+GGG - chr1_2:2681578-2681597 None:intergenic 25.0%
! AATCTAAATGCCTAATAGTA+AGG - chr1_2:2681579-2681598 None:intergenic 25.0%
!!! TGATAAGTTGATAACTTCTA+TGG + chr1_2:2681640-2681659 Msa0049390:intron 25.0%
!! TCAGATTTTCTTGCAAAATT+TGG + chr1_2:2681666-2681685 Msa0049390:intron 25.0%
! ATATTGAATGTTCTTCTAGA+TGG + chr1_2:2681994-2682013 Msa0049390:intron 25.0%
!!! ACTTTGTAGTATGTTGTTTT+CGG + chr1_2:2682067-2682086 Msa0049390:intron 25.0%
!!! TTTATTGTTGATGGTTAGTT+AGG + chr1_2:2682335-2682354 Msa0049390:intron 25.0%
! GTTTCATTGTCTTGAATTTA+CGG + chr1_2:2682486-2682505 Msa0049390:intron 25.0%
! TTTCATTGTCTTGAATTTAC+GGG + chr1_2:2682487-2682506 Msa0049390:intron 25.0%
! ATGTGCATATGATTATAAAG+GGG - chr1_2:2682550-2682569 None:intergenic 25.0%
! CAATGTGCATATGATTATAA+AGG - chr1_2:2682552-2682571 None:intergenic 25.0%
! AAAACATACCTAAAATGGAT+AGG - chr1_2:2682605-2682624 None:intergenic 25.0%
! AGCTTAAAACATACCTAAAA+TGG - chr1_2:2682610-2682629 None:intergenic 25.0%
!! ATTCAGAAAATTTGAGACTT+AGG - chr1_2:2682698-2682717 None:intergenic 25.0%
!!! TTTAGGACTTCCATTATTTT+TGG + chr1_2:2682746-2682765 Msa0049390:intron 25.0%
!!! TTAGGACTTCCATTATTTTT+GGG + chr1_2:2682747-2682766 Msa0049390:intron 25.0%
! CAAAAGAAACCCAAAAATAA+TGG - chr1_2:2682759-2682778 None:intergenic 25.0%
! GTTAAATGGTTAAATGTATG+CGG + chr1_2:2682865-2682884 Msa0049390:intron 25.0%
! TTACAAAGTTAAATATGTGC+TGG + chr1_2:2682913-2682932 Msa0049390:intron 25.0%
!!! GGAATATCATTTGTTTTGTT+AGG + chr1_2:2682934-2682953 Msa0049390:intron 25.0%
!! ACTCTTTTATGGTGATTTAT+TGG + chr1_2:2683227-2683246 Msa0049390:intron 25.0%
! TGCCTAAAAGTGATATATTT+CGG + chr1_2:2683717-2683736 Msa0049390:intron 25.0%
!!! TACCGAAATATATCACTTTT+AGG - chr1_2:2683722-2683741 None:intergenic 25.0%
!! TTTTAGAGAACAATCTTGGT+TGG + chr1_2:2680840-2680859 Msa0049390:five_prime_UTR 30.0%
! AATCTTGGTTGGAACAATTT+TGG + chr1_2:2680851-2680870 Msa0049390:five_prime_UTR 30.0%
! AGCATAATCCCTTTTCATAT+AGG + chr1_2:2681128-2681147 Msa0049390:intron 30.0%
! GCATAATCCCTTTTCATATA+GGG + chr1_2:2681129-2681148 Msa0049390:intron 30.0%
AAACGAACCCTATATGAAAA+GGG - chr1_2:2681139-2681158 None:intergenic 30.0%
AAAACGAACCCTATATGAAA+AGG - chr1_2:2681140-2681159 None:intergenic 30.0%
GATTATTCATAAGCTTGCAA+AGG - chr1_2:2681282-2681301 None:intergenic 30.0%
GCTTATTTATAACCATTCAC+GGG - chr1_2:2681372-2681391 None:intergenic 30.0%
CTGAAAACAACTTATGAACA+TGG + chr1_2:2681489-2681508 Msa0049390:intron 30.0%
!! GATTTAGCTTTAATAGAGGT+TGG + chr1_2:2681595-2681614 Msa0049390:intron 30.0%
TTGCAAAATTTGGTATGCTA+AGG + chr1_2:2681676-2681695 Msa0049390:intron 30.0%
AAGAAAGAAATGAGAACTAC+AGG - chr1_2:2681818-2681837 None:intergenic 30.0%
CATGAACTAACGAAATATCT+TGG - chr1_2:2681867-2681886 None:intergenic 30.0%
! CTGTAGGTATTTCATCATTT+TGG + chr1_2:2681899-2681918 Msa0049390:intron 30.0%
! TAGGTATTTCATCATTTTGG+TGG + chr1_2:2681902-2681921 Msa0049390:intron 30.0%
TTGTGTTTAAGCGAATCATT+GGG + chr1_2:2681947-2681966 Msa0049390:intron 30.0%
! AAACAATGAAGTACTCGTAT+CGG - chr1_2:2682166-2682185 None:intergenic 30.0%
ATCAATCGAATCAATCATTG+AGG + chr1_2:2682312-2682331 Msa0049390:intron 30.0%
TCATTGAGGTTTATTGTTGA+TGG + chr1_2:2682326-2682345 Msa0049390:intron 30.0%
TGTGCATATGATTATAAAGG+GGG - chr1_2:2682549-2682568 None:intergenic 30.0%
! TTTTGTAGCCTATCCATTTT+AGG + chr1_2:2682594-2682613 Msa0049390:intron 30.0%
CTTTAGTACTACAATCCTTA+TGG + chr1_2:2682628-2682647 Msa0049390:intron 30.0%
!!! TTTTATTTTGACGTTCCGTT+TGG + chr1_2:2682790-2682809 Msa0049390:intron 30.0%
AGAACATAAACACAACCAAA+CGG - chr1_2:2682808-2682827 None:intergenic 30.0%
GATTCTGTTACGAAGTTAAA+TGG + chr1_2:2682851-2682870 Msa0049390:intron 30.0%
GTTCAGTAAAATTATTGTGG+CGG - chr1_2:2683071-2683090 None:intergenic 30.0%
CTGAACAATATCATATGACT+CGG + chr1_2:2683085-2683104 Msa0049390:intron 30.0%
! AGATGCAATGAACTCTTTTA+TGG + chr1_2:2683216-2683235 Msa0049390:intron 30.0%
TATGGTGATTTATTGGTATG+TGG + chr1_2:2683234-2683253 Msa0049390:intron 30.0%
!! TGTTTGTTTGTTTGTTGTGA+TGG + chr1_2:2683262-2683281 Msa0049390:intron 30.0%
!! TGTTTTTCAATGTGCAAACA+AGG + chr1_2:2683298-2683317 Msa0049390:intron 30.0%
TAATACCCTTGATCAAATGA+TGG + chr1_2:2683400-2683419 Msa0049390:CDS 30.0%
ATCATCCATCATTTGATCAA+GGG - chr1_2:2683408-2683427 None:intergenic 30.0%
CAGTAGTACAAGAAGAAATA+AGG + chr1_2:2683483-2683502 Msa0049390:CDS 30.0%
ATTTCAGAGAACTATATCTC+AGG + chr1_2:2683781-2683800 Msa0049390:intron 30.0%
GGCTATCTATGATGTATATT+AGG + chr1_2:2683909-2683928 Msa0049390:three_prime_UTR 30.0%
! TTTAAAGTCATGCCCATATT+TGG - chr1_2:2684029-2684048 None:intergenic 30.0%
!! TTAGCATTTTCAAGCACTAT+TGG + chr1_2:2684155-2684174 Msa0049390:three_prime_UTR 30.0%
!! GTAATGATTTTGGTGAGTTG+TGG - chr1_2:2680923-2680942 None:intergenic 35.0%
! ATGACACAGCGTAATGATTT+TGG - chr1_2:2680933-2680952 None:intergenic 35.0%
AAAATCATTACGCTGTGTCA+TGG + chr1_2:2680932-2680951 Msa0049390:exon 35.0%
GGTAAATTCCTCTCAATCAT+TGG + chr1_2:2680953-2680972 Msa0049390:intron 35.0%
AAATCTGGTAAGCAACACTT+AGG + chr1_2:2681090-2681109 Msa0049390:intron 35.0%
CAAAAATAAGCCGATCCAAA+CGG + chr1_2:2681345-2681364 Msa0049390:intron 35.0%
TATAAGCTATCCAGAAGATG+TGG + chr1_2:2681459-2681478 Msa0049390:intron 35.0%
!! TGGCATAAGTTGTTTTCACA+AGG + chr1_2:2681509-2681528 Msa0049390:intron 35.0%
!! GGCATAAGTTGTTTTCACAA+GGG + chr1_2:2681510-2681529 Msa0049390:intron 35.0%
!! TTAGCTTTAATAGAGGTTGG+TGG + chr1_2:2681598-2681617 Msa0049390:intron 35.0%
AATGAGAACTACAGGTTCTA+TGG - chr1_2:2681810-2681829 None:intergenic 35.0%
!!! TTGGTGGATTTTGTACGTTT+AGG + chr1_2:2681918-2681937 Msa0049390:intron 35.0%
GTTGTGTTTAAGCGAATCAT+TGG + chr1_2:2681946-2681965 Msa0049390:intron 35.0%
! AACAATGAAGTACTCGTATC+GGG - chr1_2:2682165-2682184 None:intergenic 35.0%
! TTGATGGTTAGTTAGGACTT+AGG + chr1_2:2682342-2682361 Msa0049390:intron 35.0%
! CAGTTGCATTTCTACCATTT+TGG + chr1_2:2682434-2682453 Msa0049390:intron 35.0%
AAAAGAGATGAGCTCCAAAA+TGG - chr1_2:2682451-2682470 None:intergenic 35.0%
GTGCATATGATTATAAAGGG+GGG - chr1_2:2682548-2682567 None:intergenic 35.0%
AGGCACTAGAAAACTACAAA+TGG - chr1_2:2682678-2682697 None:intergenic 35.0%
ACATAACATAGAGTGGAACA+TGG + chr1_2:2682961-2682980 Msa0049390:intron 35.0%
CATCATCCATCATTTGATCA+AGG - chr1_2:2683409-2683428 None:intergenic 35.0%
GAAAAAAAACCCATAGCTGA+TGG - chr1_2:2683616-2683635 None:intergenic 35.0%
AGAATTATCAACAAGCATGC+TGG + chr1_2:2683846-2683865 Msa0049390:three_prime_UTR 35.0%
GAATTATCAACAAGCATGCT+GGG + chr1_2:2683847-2683866 Msa0049390:three_prime_UTR 35.0%
! TATGGGCATGACTTTAAAAG+TGG + chr1_2:2684031-2684050 Msa0049390:three_prime_UTR 35.0%
TTGGTGAGTTGTGGAAAACA+CGG - chr1_2:2680914-2680933 None:intergenic 40.0%
CAGCTTATTTCCACATCTTC+TGG - chr1_2:2681472-2681491 None:intergenic 40.0%
CCTAATAGTAAGGGTCTCTA+TGG - chr1_2:2681569-2681588 None:intergenic 40.0%
CCATAGAGACCCTTACTATT+AGG + chr1_2:2681566-2681585 Msa0049390:intron 40.0%
TACAGGTTCTATGGTAAGAG+AGG - chr1_2:2681801-2681820 None:intergenic 40.0%
!! GTTCTTCTAGATGGATTGTG+TGG + chr1_2:2682003-2682022 Msa0049390:intron 40.0%
! TCATGTTGCTATTGACACCA+GGG - chr1_2:2682124-2682143 None:intergenic 40.0%
ATAGCAACATGACATGACTC+TGG + chr1_2:2682132-2682151 Msa0049390:intron 40.0%
TAGCAACATGACATGACTCT+GGG + chr1_2:2682133-2682152 Msa0049390:intron 40.0%
CCCTATTCAGCTTAGTTGTT+GGG - chr1_2:2682211-2682230 None:intergenic 40.0%
CCCAACAACTAAGCTGAATA+GGG + chr1_2:2682208-2682227 Msa0049390:intron 40.0%
TCCCTATTCAGCTTAGTTGT+TGG - chr1_2:2682212-2682231 None:intergenic 40.0%
CTCATATTCCCACTCACTAA+CGG - chr1_2:2682271-2682290 None:intergenic 40.0%
GTTAGTGAGTGGGAATATGA+GGG + chr1_2:2682270-2682289 Msa0049390:intron 40.0%
TTAGGCGAGTAGTTTCTCTT+TGG + chr1_2:2682360-2682379 Msa0049390:intron 40.0%
!! GTAGAAGGTATTTCCATAGC+AGG - chr1_2:2682526-2682545 None:intergenic 40.0%
TGATTATAAAGGGGGGTAGA+AGG - chr1_2:2682541-2682560 None:intergenic 40.0%
! ATTGCCAATGCTTTTACGAG+TGG + chr1_2:2682568-2682587 Msa0049390:intron 40.0%
! AATGCCACTCGTAAAAGCAT+TGG - chr1_2:2682575-2682594 None:intergenic 40.0%
AGTACTACAATCCTTATGGC+TGG + chr1_2:2682632-2682651 Msa0049390:intron 40.0%
CGGTAGAATAAGTAGCTACA+TGG + chr1_2:2682885-2682904 Msa0049390:intron 40.0%
AGGACACACATAACATAGAG+TGG + chr1_2:2682954-2682973 Msa0049390:intron 40.0%
CAGTAAAATTATTGTGGCGG+TGG - chr1_2:2683068-2683087 None:intergenic 40.0%
GAGTTCATTGCATCTCACAA+TGG - chr1_2:2683211-2683230 None:intergenic 40.0%
!! TTGATAGTGCAGTGCAAAAG+TGG + chr1_2:2683352-2683371 Msa0049390:intron 40.0%
! TGTTTTCTCTCACACCATAC+TGG - chr1_2:2683461-2683480 None:intergenic 40.0%
AGTATGGTGTGAGAGAAAAC+AGG + chr1_2:2683460-2683479 Msa0049390:CDS 40.0%
TCTGCAACTTATGTCAGTTC+AGG + chr1_2:2683571-2683590 Msa0049390:CDS 40.0%
CAACTTATGTCAGTTCAGGT+AGG + chr1_2:2683575-2683594 Msa0049390:intron 40.0%
! GCTGATGGACAAAGAGTTAA+TGG - chr1_2:2683601-2683620 None:intergenic 40.0%
AACTCTTTGTCCATCAGCTA+TGG + chr1_2:2683603-2683622 Msa0049390:intron 40.0%
ACTCTTTGTCCATCAGCTAT+GGG + chr1_2:2683604-2683623 Msa0049390:intron 40.0%
GGCACATCAAAGCCAAATAT+GGG + chr1_2:2684014-2684033 Msa0049390:three_prime_UTR 40.0%
ATCAATTGCTCGAGCAGGAA+TGG - chr1_2:2680879-2680898 None:intergenic 45.0%
CATGTTGTTGATCGAGCTGT+AGG + chr1_2:2681883-2681902 Msa0049390:intron 45.0%
TTAAGCGAATCATTGGGCAG+TGG + chr1_2:2681953-2681972 Msa0049390:intron 45.0%
AATATGATCCTCACATCCCC+TGG + chr1_2:2682104-2682123 Msa0049390:intron 45.0%
! CATGTTGCTATTGACACCAG+GGG - chr1_2:2682123-2682142 None:intergenic 45.0%
! GTCATGTTGCTATTGACACC+AGG - chr1_2:2682125-2682144 None:intergenic 45.0%
GCCCAACAACTAAGCTGAAT+AGG + chr1_2:2682207-2682226 Msa0049390:intron 45.0%
ACTCAACTCCGTTAGTGAGT+GGG + chr1_2:2682260-2682279 Msa0049390:intron 45.0%
CGTTAGTGAGTGGGAATATG+AGG + chr1_2:2682269-2682288 Msa0049390:intron 45.0%
!! TGGTTAAGAGCATTGTGCTC+TGG + chr1_2:2682380-2682399 Msa0049390:intron 45.0%
!! AAGAGCATTGTGCTCTGGTT+AGG + chr1_2:2682385-2682404 Msa0049390:intron 45.0%
GGTTAGGAGTTCTTCAACTC+TGG + chr1_2:2682401-2682420 Msa0049390:intron 45.0%
TTCCATCACAATGCCTGCTA+TGG + chr1_2:2682510-2682529 Msa0049390:intron 45.0%
! TTCCATAGCAGGCATTGTGA+TGG - chr1_2:2682515-2682534 None:intergenic 45.0%
TCATCAGTTCTCCAGCCATA+AGG - chr1_2:2682646-2682665 None:intergenic 45.0%
!!! CATACTGGTTTTTGTGCTGC+AGG - chr1_2:2683446-2683465 None:intergenic 45.0%
CTGCAGCACAAAAACCAGTA+TGG + chr1_2:2683444-2683463 Msa0049390:CDS 45.0%
!! GCGACATAGGTACTTTTTGC+AGG - chr1_2:2683524-2683543 None:intergenic 45.0%
GAGAACTATATCTCAGGAGC+AGG + chr1_2:2683787-2683806 Msa0049390:intron 45.0%
TATATCTCAGGAGCAGGAGT+TGG + chr1_2:2683793-2683812 Msa0049390:CDS 45.0%
!!! GAGTTGGAGTTGCTTTTTGC+TGG + chr1_2:2683809-2683828 Msa0049390:CDS 45.0%
CGGCACATCAAAGCCAAATA+TGG + chr1_2:2684013-2684032 Msa0049390:three_prime_UTR 45.0%
TCCTGCTCGAGCAATTGATC+AGG + chr1_2:2680880-2680899 Msa0049390:five_prime_UTR 50.0%
TCCTGATCAATTGCTCGAGC+AGG - chr1_2:2680884-2680903 None:intergenic 50.0%
ATTCACGGGTCCGTTTGGAT+CGG - chr1_2:2681358-2681377 None:intergenic 50.0%
TAACCATTCACGGGTCCGTT+TGG - chr1_2:2681363-2681382 None:intergenic 50.0%
TATTGACACCAGGGGATGTG+AGG - chr1_2:2682115-2682134 None:intergenic 50.0%
!! GTTGTTGGGCTCTGAAGCAT+GGG - chr1_2:2682197-2682216 None:intergenic 50.0%
!! AGTTGTTGGGCTCTGAAGCA+TGG - chr1_2:2682198-2682217 None:intergenic 50.0%
AGGGAAGTTAGTTGCTGACG+TGG + chr1_2:2682227-2682246 Msa0049390:intron 50.0%
! AACGGAGTTGAGTGGCTCTA+AGG - chr1_2:2682253-2682272 None:intergenic 50.0%
CACTCACTAACGGAGTTGAG+TGG - chr1_2:2682261-2682280 None:intergenic 50.0%
CACTCAACTCCGTTAGTGAG+TGG + chr1_2:2682259-2682278 Msa0049390:intron 50.0%
TGGGAATATGAGGGCAGCAA+GGG + chr1_2:2682279-2682298 Msa0049390:intron 50.0%
CTTGAGACGATGTGCGACAT+AGG - chr1_2:2683537-2683556 None:intergenic 50.0%
CTATGTCGCACATCGTCTCA+AGG + chr1_2:2683535-2683554 Msa0049390:CDS 50.0%
GAGTTGTGGAAAACACGGCG+AGG - chr1_2:2680909-2680928 None:intergenic 55.0%
GATCCAAACGGACCCGTGAA+TGG + chr1_2:2681357-2681376 Msa0049390:intron 55.0%
GTGGGAATATGAGGGCAGCA+AGG + chr1_2:2682278-2682297 Msa0049390:intron 55.0%
AACCTTCAAGCATGCCTGCG+CGG - chr1_2:2683940-2683959 None:intergenic 55.0%
TGAGGGCAGCAAGGGTAGTG+AGG + chr1_2:2682287-2682306 Msa0049390:intron 60.0%
GAGGGCAGCAAGGGTAGTGA+GGG + chr1_2:2682288-2682307 Msa0049390:intron 60.0%
TATATTAGGCGCAGCCGCGC+AGG + chr1_2:2683923-2683942 Msa0049390:three_prime_UTR 60.0%
AGCCGCGCAGGCATGCTTGA+AGG + chr1_2:2683935-2683954 Msa0049390:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 2680839 2684194 2680839 ID=Msa0049390;Name=Msa0049390
chr1_2 mRNA 2680839 2684194 2680839 ID=Msa0049390-mRNA-1;Parent=Msa0049390;Name=Msa0049390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=112|1|1|1|0.5|1|3|350|97
chr1_2 exon 2680839 2680953 2680839 ID=Msa0049390-mRNA-1:exon:565;Parent=Msa0049390-mRNA-1
chr1_2 exon 2683359 2683592 2683359 ID=Msa0049390-mRNA-1:exon:566;Parent=Msa0049390-mRNA-1
chr1_2 exon 2683788 2684194 2683788 ID=Msa0049390-mRNA-1:exon:567;Parent=Msa0049390-mRNA-1
chr1_2 five_prime_UTR 2680839 2680950 2680839 ID=Msa0049390-mRNA-1:five_prime_utr;Parent=Msa0049390-mRNA-1
chr1_2 CDS 2680951 2680953 2680951 ID=Msa0049390-mRNA-1:cds;Parent=Msa0049390-mRNA-1
chr1_2 CDS 2683359 2683592 2683359 ID=Msa0049390-mRNA-1:cds;Parent=Msa0049390-mRNA-1
chr1_2 CDS 2683788 2683844 2683788 ID=Msa0049390-mRNA-1:cds;Parent=Msa0049390-mRNA-1
chr1_2 three_prime_UTR 2683845 2684194 2683845 ID=Msa0049390-mRNA-1:three_prime_utr;Parent=Msa0049390-mRNA-1
Gene Sequence

>Msa0049390

ATGTGCAGTGCAAAAGTGGATTCCCTTGATAATTTAAACAGTAGTAATACCCTTGATCAAATGATGGATGATGCAGCTACTCATGAACCTGCAGCACAAAAACCAGTATGGTGTGAGAGAAAACAGGCAGTAGTACAAGAAGAAATAAGGAAAATGAATCAATTGCCTGCAAAAAGTACCTATGTCGCACATCGTCTCAAGGTTCTTAATAAAATTCTGCAACTTATGTCAGTTCAGAGAACTATATCTCAGGAGCAGGAGTTGGAGTTGCTTTTTGCTGGTTTGTCTCTGTGA
Protein sequence

>Msa0049390

MCSAKVDSLDNLNSSNTLDQMMDDAATHEPAAQKPVWCERKQAVVQEEIRKMNQLPAKSTYVAHRLKVLNKILQLMSVQRTISQEQELELLFAGLSL*