AlfalfaGEDB | Msa0716530

M. sativa cultivar zhongmu-4 / Msa0716530

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716530	AES94947.2	68.750	64	17	1	1	61	1	64	5.56e-17	79.0

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716530	G7KDJ6	68.750	64	17	1	1	61	1	64	2.65e-17	79.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011170	Msa0716530	0.837913	3.925692e-57	-8.615850e-47
Msa0014710	Msa0716530	0.808522	2.972167e-50	-8.615850e-47
Msa0015390	Msa0716530	0.800168	1.635433e-48	-8.615850e-47
Msa0017070	Msa0716530	0.818723	1.689394e-52	-8.615850e-47
Msa0031340	Msa0716530	0.800348	1.503077e-48	-8.615850e-47
Msa0036140	Msa0716530	0.818473	1.924451e-52	-8.615850e-47
Msa0036920	Msa0716530	0.801671	8.066907e-49	-8.615850e-47
Msa0039330	Msa0716530	0.828963	6.710725e-55	-8.615850e-47
Msa0044260	Msa0716530	0.814200	1.739067e-51	-8.615850e-47
Msa0050380	Msa0716530	0.802385	5.751976e-49	-8.615850e-47
Msa0081930	Msa0716530	0.812401	4.319278e-51	-8.615850e-47
Msa0095560	Msa0716530	0.826541	2.563257e-54	-8.615850e-47
Msa0102120	Msa0716530	0.808227	3.434573e-50	-8.615850e-47
Msa0622650	Msa0716530	0.837104	6.327166e-57	-8.615850e-47
Msa0652940	Msa0716530	0.833789	4.357996e-56	-8.615850e-47
Msa0657970	Msa0716530	0.818690	1.718285e-52	-8.615850e-47
Msa0658570	Msa0716530	0.801648	8.153752e-49	-8.615850e-47
Msa0663380	Msa0716530	0.807310	5.381784e-50	-8.615850e-47
Msa0672690	Msa0716530	0.816131	6.478346e-52	-8.615850e-47
Msa0688070	Msa0716530	0.808381	3.184724e-50	-8.615850e-47
Msa0688270	Msa0716530	0.800874	1.174170e-48	-8.615850e-47
Msa0689800	Msa0716530	0.872520	2.985361e-67	-8.615850e-47
Msa0692230	Msa0716530	0.800173	1.632180e-48	-8.615850e-47
Msa0693380	Msa0716530	0.832255	1.049254e-55	-8.615850e-47
Msa0695430	Msa0716530	0.800335	1.512423e-48	-8.615850e-47
Msa0701900	Msa0716530	0.812022	5.224393e-51	-8.615850e-47
Msa0716530	Msa0735170	0.802598	5.199059e-49	-8.615850e-47
Msa0716530	Msa0740510	0.825681	4.105078e-54	-8.615850e-47
Msa0716530	Msa0744040	0.808882	2.488949e-50	-8.615850e-47
Msa0716530	Msa0745790	0.806984	6.306056e-50	-8.615850e-47
Msa0716530	Msa0753720	0.825593	4.307262e-54	-8.615850e-47
Msa0716530	Msa0760130	0.826194	3.101086e-54	-8.615850e-47
Msa0716530	Msa0766630	0.803328	3.674027e-49	-8.615850e-47
Msa0716530	Msa0790510	0.818214	2.203255e-52	-8.615850e-47
Msa0716530	Msa0796700	0.803560	3.289327e-49	-8.615850e-47
Msa0716530	Msa0798860	0.802487	5.481351e-49	-8.615850e-47
Msa0716530	Msa0806230	0.804932	1.703995e-49	-8.615850e-47
Msa0716530	Msa0806240	0.802390	5.740402e-49	-8.615850e-47
Msa0716530	Msa0806250	0.809094	2.242371e-50	-8.615850e-47
Msa0716530	Msa0806260	0.803117	4.063118e-49	-8.615850e-47
Msa0716530	Msa0806280	0.809427	1.902920e-50	-8.615850e-47
Msa0716530	Msa0807030	0.838901	2.183976e-57	-8.615850e-47
Msa0716530	Msa0813400	0.814880	1.230000e-51	-8.615850e-47
Msa0716530	Msa0820770	0.827943	1.183083e-54	-8.615850e-47
Msa0716530	Msa0825340	0.807434	5.064237e-50	-8.615850e-47
Msa0716530	Msa0832970	0.804417	2.182394e-49	-8.615850e-47
Msa0716530	Msa0844240	0.813382	2.633533e-51	-8.615850e-47
Msa0716530	Msa0861090	0.822195	2.697391e-53	-8.615850e-47
Msa0716530	Msa0861540	0.806369	8.507812e-50	-8.615850e-47
Msa0716530	Msa0880950	0.815699	8.088250e-52	-8.615850e-47
Msa0716530	Msa0892080	0.801007	1.103155e-48	-8.615850e-47
Msa0716530	Msa0907690	0.804166	2.461351e-49	-8.615850e-47
Msa0716530	Msa0927290	0.838056	3.607398e-57	-8.615850e-47
Msa0716530	Msa0931520	0.808681	2.748409e-50	-8.615850e-47
Msa0716530	Msa0935670	0.829421	5.197687e-55	-8.615850e-47
Msa0716530	Msa0953550	0.804815	1.802098e-49	-8.615850e-47
Msa0716530	Msa0954550	0.806689	7.280013e-50	-8.615850e-47
Msa0716530	Msa0969520	0.805034	1.621578e-49	-8.615850e-47
Msa0716530	Msa0972630	0.805723	1.163295e-49	-8.615850e-47
Msa0716530	Msa0976670	0.811399	7.137090e-51	-8.615850e-47
Msa0716530	Msa0989360	0.852664	3.980522e-61	-8.615850e-47
Msa0716530	Msa1001180	0.826219	3.059710e-54	-8.615850e-47
Msa0716530	Msa1014540	0.816412	5.604624e-52	-8.615850e-47
Msa0716530	Msa1015730	0.804070	2.577242e-49	-8.615850e-47
Msa0716530	Msa1015790	0.811728	6.053563e-51	-8.615850e-47
Msa0716530	Msa1015800	0.801968	7.009240e-49	-8.615850e-47
Msa0716530	Msa1015810	0.803258	3.798298e-49	-8.615850e-47
Msa0716530	Msa1034300	0.801307	9.575786e-49	-8.615850e-47
Msa0716530	Msa1059570	0.820735	5.862556e-53	-8.615850e-47
Msa0716530	Msa1071100	0.814877	1.231809e-51	-8.615850e-47
Msa0716530	Msa1071110	0.803070	4.155025e-49	-8.615850e-47
Msa0716530	Msa1075220	-0.822238	2.636488e-53	-8.615850e-47
Msa0716530	Msa1081590	0.834791	2.443326e-56	-8.615850e-47
Msa0716530	Msa1082500	0.831379	1.725605e-55	-8.615850e-47
Msa0716530	Msa1083840	0.810059	1.391490e-50	-8.615850e-47
Msa0716530	Msa1085300	0.810484	1.126718e-50	-8.615850e-47
Msa0716530	Msa1090320	0.828264	9.901092e-55	-8.615850e-47
Msa0716530	Msa1091830	0.804549	2.048369e-49	-8.615850e-47
Msa0716530	Msa1098420	0.803573	3.268862e-49	-8.615850e-47
Msa0716530	Msa1105450	0.806680	7.314498e-50	-8.615850e-47
Msa0716530	Msa1116130	-0.816200	6.251501e-52	-8.615850e-47
Msa0716530	Msa1121490	0.806759	7.038791e-50	-8.615850e-47
Msa0716530	Msa1139000	-0.830070	3.611377e-55	-8.615850e-47
Msa0716530	Msa1143270	0.809216	2.111209e-50	-8.615850e-47
Msa0716530	Msa1148050	0.820886	5.410950e-53	-8.615850e-47
Msa0716530	Msa1162550	-0.829419	5.202135e-55	-8.615850e-47
Msa0716530	Msa1174040	0.819483	1.134100e-52	-8.615850e-47
Msa0716530	Msa1224480	0.810822	9.521292e-51	-8.615850e-47
Msa0716530	Msa1228590	0.810276	1.249199e-50	-8.615850e-47
Msa0716530	Msa1233890	0.821043	4.980555e-53	-8.615850e-47
Msa0716530	Msa1246500	0.819926	8.985439e-53	-8.615850e-47
Msa0716530	Msa1246510	0.816104	6.566112e-52	-8.615850e-47
Msa0716530	Msa1268700	0.816028	6.831114e-52	-8.615850e-47
Msa0716530	Msa1270610	0.819580	1.077771e-52	-8.615850e-47
Msa0716530	Msa1283860	0.803580	3.257576e-49	-8.615850e-47
Msa0716530	Msa1284680	0.800297	1.539940e-48	-8.615850e-47
Msa0716530	Msa1287480	0.802232	6.184918e-49	-8.615850e-47
Msa0716530	Msa1297290	0.818279	2.129323e-52	-8.615850e-47
Msa0716530	Msa1307070	0.829372	5.341095e-55	-8.615850e-47
Msa0716530	Msa1316200	0.828572	8.343218e-55	-8.615850e-47
Msa0716530	Msa1322890	0.805417	1.348203e-49	-8.615850e-47
Msa0716530	Msa1329700	0.821040	4.988516e-53	-8.615850e-47
Msa0716530	Msa1331000	0.807303	5.398739e-50	-8.615850e-47
Msa0716530	Msa1359040	0.801756	7.746815e-49	-8.615850e-47
Msa0716530	Msa1372760	0.806801	6.896726e-50	-8.615850e-47
Msa0716530	Msa1376890	0.807112	5.927121e-50	-8.615850e-47
Msa0716530	Msa1384350	0.805376	1.375481e-49	-8.615850e-47
Msa0716530	Msa1385000	0.819015	1.449950e-52	-8.615850e-47
Msa0716530	Msa1390050	0.806595	7.622484e-50	-8.615850e-47
Msa0716530	Msa1392260	0.807105	5.945343e-50	-8.615850e-47
Msa0716530	Msa1409700	0.817434	3.304161e-52	-8.615850e-47
Msa0716530	Msa1412890	0.802444	5.593871e-49	-8.615850e-47
Msa0716530	Msa1420290	0.849016	4.236835e-60	-8.615850e-47
Msa0716530	Msa1423880	0.800054	1.725587e-48	-8.615850e-47
Msa0716530	Msa1424680	0.804324	2.281090e-49	-8.615850e-47
Msa0716530	Msa1436480	0.815260	1.012492e-51	-8.615850e-47
Msa0716530	Msa1454120	0.834762	2.484748e-56	-8.615850e-47
Msa0716530	Msa1458550	0.810542	1.094314e-50	-8.615850e-47
Msa0716530	Msa1460050	0.816558	5.199338e-52	-8.615850e-47
Msa0716530	Msa1460830	0.805027	1.627537e-49	-8.615850e-47
Msa0122590	Msa0716530	0.800878	1.172332e-48	-8.615850e-47
Msa0128020	Msa0716530	0.800895	1.162682e-48	-8.615850e-47
Msa0143560	Msa0716530	0.839551	1.480527e-57	-8.615850e-47
Msa0166870	Msa0716530	0.847718	9.686475e-60	-8.615850e-47
Msa0175390	Msa0716530	0.803879	2.824171e-49	-8.615850e-47
Msa0188830	Msa0716530	0.802078	6.655096e-49	-8.615850e-47
Msa0201070	Msa0716530	0.804304	2.303243e-49	-8.615850e-47
Msa0206790	Msa0716530	0.820242	7.608443e-53	-8.615850e-47
Msa0210450	Msa0716530	0.802131	6.490213e-49	-8.615850e-47
Msa0212990	Msa0716530	0.814695	1.351618e-51	-8.615850e-47
Msa0220580	Msa0716530	0.849408	3.296465e-60	-8.615850e-47
Msa0225320	Msa0716530	0.800765	1.235948e-48	-8.615850e-47
Msa0233300	Msa0716530	0.801039	1.086524e-48	-8.615850e-47
Msa0237660	Msa0716530	0.808475	3.041119e-50	-8.615850e-47
Msa0366970	Msa0716530	0.800993	1.110220e-48	-8.615850e-47
Msa0376590	Msa0716530	0.805072	1.592917e-49	-8.615850e-47
Msa0379640	Msa0716530	0.828167	1.044859e-54	-8.615850e-47
Msa0389340	Msa0716530	0.811982	5.332172e-51	-8.615850e-47
Msa0417270	Msa0716530	0.810474	1.131980e-50	-8.615850e-47
Msa0453640	Msa0716530	0.817815	2.710763e-52	-8.615850e-47
Msa0242430	Msa0716530	0.809343	1.982693e-50	-8.615850e-47
Msa0265380	Msa0716530	0.802932	4.437481e-49	-8.615850e-47
Msa0266690	Msa0716530	0.826028	3.396411e-54	-8.615850e-47
Msa0266720	Msa0716530	0.847044	1.483379e-59	-8.615850e-47
Msa0270100	Msa0716530	0.802149	6.432645e-49	-8.615850e-47
Msa0285450	Msa0716530	0.804337	2.267352e-49	-8.615850e-47
Msa0288100	Msa0716530	0.847656	1.007184e-59	-8.615850e-47
Msa0309770	Msa0716530	0.815127	1.084051e-51	-8.615850e-47
Msa0313560	Msa0716530	0.842030	3.315321e-58	-8.615850e-47
Msa0315530	Msa0716530	0.805755	1.145187e-49	-8.615850e-47
Msa0330340	Msa0716530	0.807341	5.300092e-50	-8.615850e-47
Msa0339620	Msa0716530	0.809663	1.692610e-50	-8.615850e-47
Msa0345970	Msa0716530	0.801516	8.679071e-49	-8.615850e-47
Msa0363790	Msa0716530	0.800608	1.330811e-48	-8.615850e-47
Msa0484780	Msa0716530	0.819505	1.120978e-52	-8.615850e-47
Msa0488560	Msa0716530	0.809010	2.336878e-50	-8.615850e-47
Msa0493630	Msa0716530	0.808074	3.703039e-50	-8.615850e-47
Msa0503060	Msa0716530	0.804847	1.774611e-49	-8.615850e-47
Msa0512750	Msa0716530	0.821040	4.987415e-53	-8.615850e-47
Msa0512900	Msa0716530	0.800121	1.672125e-48	-8.615850e-47
Msa0515010	Msa0716530	0.801944	7.089173e-49	-8.615850e-47
Msa0545210	Msa0716530	0.818152	2.275241e-52	-8.615850e-47
Msa0584860	Msa0716530	0.805858	1.089376e-49	-8.615850e-47
Msa0585230	Msa0716530	0.809498	1.836583e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716530	MtrunA17_Chr5g0403961	67.213	61	14	1	1	61	1	55	1.16e-19	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 90 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGAAGATTGGAAGGTCTTT+AGG	0.227758	5_1:+10005664	Msa0716530:three_prime_UTR
CATATCGTTCAACCGTTTCT+AGG	0.229849	5_1:+10005054	Msa0716530:five_prime_UTR
GATTCAGATGTTTCAGATCT+TGG	0.247670	5_1:+10005619	Msa0716530:three_prime_UTR
TACATGATCTACTAATTTCT+TGG	0.278933	5_1:-10006478	None:intergenic
AGAGTGAAAAGAAGCAATTC+TGG	0.285018	5_1:+10006610	Msa0716530:three_prime_UTR
TCTTTAGGGGTGGGATATAA+TGG	0.286960	5_1:+10005679	Msa0716530:three_prime_UTR
TGAAGATTGGAAGGTCTTTA+GGG	0.301169	5_1:+10005665	Msa0716530:three_prime_UTR
TGGATGTAGGATATAACTTT+AGG	0.315945	5_1:+10005699	Msa0716530:three_prime_UTR
TAGATCTCAGCGGTGAATTA+TGG	0.318563	5_1:+10005576	Msa0716530:three_prime_UTR
TTCTTTATTCATACTGTGTA+TGG	0.321435	5_1:+10006957	Msa0716530:three_prime_UTR
TAGTTTGATTGATTTACTAA+TGG	0.324398	5_1:+10007218	Msa0716530:three_prime_UTR
CTCTCTTCCATTCCTCCCAT+TGG	0.326349	5_1:-10006591	None:intergenic
TATTAATCTTGAATCAATAA+AGG	0.333488	5_1:-10005143	None:intergenic
GTAGATCCGGTGTTGAAATA+TGG	0.336011	5_1:+10005506	Msa0716530:CDS
GTCAGGTGCGCTTGCTTGGC+AGG	0.350358	5_1:+10005797	Msa0716530:three_prime_UTR
AACGGTTGAACGATATGAAT+TGG	0.363820	5_1:-10005048	None:intergenic
TTTGTTCAAAATGCAAATAC+AGG	0.364368	5_1:-10007083	None:intergenic
TATGTTTATATGATGAAGAT+TGG	0.365484	5_1:+10005652	Msa0716530:three_prime_UTR
CCATGTCAGGTGCGCTTGCT+TGG	0.370305	5_1:+10005793	Msa0716530:three_prime_UTR
AAATTAGTAGATCATGTATC+AGG	0.402589	5_1:+10006483	Msa0716530:three_prime_UTR
CATCCGACCTTTGCCATGTC+AGG	0.416093	5_1:+10005780	Msa0716530:three_prime_UTR
GCAAATACAGGCTTTATTCT+CGG	0.425412	5_1:-10007071	None:intergenic
CAAATACAGGCTTTATTCTC+GGG	0.427127	5_1:-10007070	None:intergenic
CAAAATCCATTATAAAAGAA+AGG	0.431202	5_1:+10005014	Msa0716530:five_prime_UTR
CATTAATGTGATTAATACTA+AGG	0.438158	5_1:+10006666	Msa0716530:three_prime_UTR
TGTAAATTCATCAAGGGACT+TGG	0.453964	5_1:+10006516	Msa0716530:three_prime_UTR
GACGAACAAGTTCACACCTC+TGG	0.455850	5_1:-10005381	None:intergenic
CCTAGTCATCTTCTCCTCAA+TGG	0.456830	5_1:-10006836	None:intergenic
ATTGAAGGAAAGAAGGAAGA+AGG	0.460468	5_1:-10005089	None:intergenic
CCAAGCAAGCGCACCTGACA+TGG	0.466895	5_1:-10005793	None:intergenic
ACTAAGGTGAAGATGACTTC+GGG	0.472109	5_1:+10006682	Msa0716530:three_prime_UTR
TTGAAGGAAAGAAGGAAGAA+GGG	0.479770	5_1:-10005088	None:intergenic
AAAATCCATTATAAAAGAAA+GGG	0.480170	5_1:+10005015	Msa0716530:five_prime_UTR
TAGACGAGATCTGAGATTGA+AGG	0.485194	5_1:-10005104	None:intergenic
AGGTGTGAACTTGTTCGTCT+TGG	0.490491	5_1:+10005385	Msa0716530:CDS
GAGTGAAAAGAAGCAATTCT+GGG	0.498652	5_1:+10006611	Msa0716530:three_prime_UTR
ATCTGAGATTGAAGGAAAGA+AGG	0.501636	5_1:-10005096	None:intergenic
AATCTTGAATCAATAAAGGT+TGG	0.501834	5_1:-10005139	None:intergenic
ATATCGTTCAACCGTTTCTA+GGG	0.506871	5_1:+10005055	Msa0716530:five_prime_UTR
CTTCTCCTCAATGGATGAAG+AGG	0.508343	5_1:-10006827	None:intergenic
GCTGAGATTAGTTGTGTTGA+CGG	0.528897	5_1:-10004979	None:intergenic
ATTTATCGACTAGCACCAAT+GGG	0.529092	5_1:+10006576	Msa0716530:three_prime_UTR
CCTTGAGGAGATCGTAGATC+CGG	0.532855	5_1:+10005493	Msa0716530:CDS
ATTGGAAGGTCTTTAGGGGT+GGG	0.533592	5_1:+10005670	Msa0716530:three_prime_UTR
AATCACATTAATGATTGAAG+AGG	0.534445	5_1:-10006656	None:intergenic
GTCATCCTCTTCATCCATTG+AGG	0.536107	5_1:+10006822	Msa0716530:three_prime_UTR
GATTGGAAGGTCTTTAGGGG+TGG	0.540908	5_1:+10005669	Msa0716530:three_prime_UTR
GGGTGGGATATAATGGATGT+AGG	0.542824	5_1:+10005686	Msa0716530:three_prime_UTR
TTTATATGATGAAGATTGGA+AGG	0.545648	5_1:+10005656	Msa0716530:three_prime_UTR
TAGCTTCGATGAATCCGTCC+GGG	0.547769	5_1:-10005977	None:intergenic
TTGAATCAATAAAGGTTGGA+AGG	0.550125	5_1:-10005135	None:intergenic
CACACACGAGAGAAGATCCC+GGG	0.550552	5_1:-10005880	None:intergenic
TTAGCTTCGATGAATCCGTC+CGG	0.551236	5_1:-10005978	None:intergenic
AAAACATGTAATCAAGGGAG+GGG	0.551933	5_1:-10006981	None:intergenic
ACTAGCACCAATGGGAGGAA+TGG	0.553780	5_1:+10006584	Msa0716530:three_prime_UTR
ATAGCTCTGTAAATTCATCA+AGG	0.557269	5_1:+10006509	Msa0716530:three_prime_UTR
GATTTATCGACTAGCACCAA+TGG	0.559545	5_1:+10006575	Msa0716530:three_prime_UTR
CGCTCTACTCACAATAGCCC+CGG	0.565882	5_1:+10005959	Msa0716530:three_prime_UTR
CAAGATTAATATAGATCCGA+TGG	0.566520	5_1:+10005155	Msa0716530:exon
TACTAAGGTGAAGATGACTT+CGG	0.568061	5_1:+10006681	Msa0716530:three_prime_UTR
GAATCGTGAAACCCTAGAAA+CGG	0.568617	5_1:-10005066	None:intergenic
CGGATGACAAGATCACTCTC+AGG	0.573514	5_1:-10005763	None:intergenic
CCATTGAGGAGAAGATGACT+AGG	0.573518	5_1:+10006836	Msa0716530:three_prime_UTR
TTGATGCGCTGAGCGTATGA+AGG	0.575533	5_1:+10005733	Msa0716530:three_prime_UTR
TTCATACGCTCAGCGCATCA+AGG	0.578674	5_1:-10005731	None:intergenic
ATCAGCATGTAGATCTCCAT+CGG	0.587084	5_1:-10005171	None:intergenic
ATAAGGAAAGGAACAAGGCA+AGG	0.588509	5_1:-10005911	None:intergenic
ACACACACGAGAGAAGATCC+CGG	0.591246	5_1:-10005881	None:intergenic
TTCCTAAGTCAAGGCTCTGG+AGG	0.594153	5_1:+10005444	Msa0716530:intron
GGGATCTTCTCTCGTGTGTG+TGG	0.604344	5_1:+10005883	Msa0716530:three_prime_UTR
ACATTAATGATTGAAGAGGA+TGG	0.615661	5_1:-10006652	None:intergenic
CACCGCTGAGATCTACAACT+CGG	0.618056	5_1:-10005568	None:intergenic
GAATCGCCATATTTCAACAC+CGG	0.620976	5_1:-10005512	None:intergenic
AAGCGCACCTGACATGGCAA+AGG	0.629352	5_1:-10005787	None:intergenic
CCGGATCTACGATCTCCTCA+AGG	0.633083	5_1:-10005493	None:intergenic
TAGCTCTGTAAATTCATCAA+GGG	0.634789	5_1:+10006510	Msa0716530:three_prime_UTR
GATTAGTTGTGTTGACGGTG+TGG	0.639961	5_1:-10004974	None:intergenic
AAAGAATAAGGAAAGGAACA+AGG	0.645363	5_1:-10005916	None:intergenic
GATGGAGATCTACATGCTGA+TGG	0.646240	5_1:+10005173	Msa0716530:exon
GCACCTGACATGGCAAAGGT+CGG	0.647464	5_1:-10005783	None:intergenic
AATCTCAAGACTCAGTAACA+AGG	0.651411	5_1:+10005999	Msa0716530:three_prime_UTR
AGCTTCGATGAATCCGTCCG+GGG	0.673406	5_1:-10005976	None:intergenic
GCATCAGATCTACGATCCAG+AGG	0.674565	5_1:+10005365	Msa0716530:CDS
GAAGATTGGAAGGTCTTTAG+GGG	0.676902	5_1:+10005666	Msa0716530:three_prime_UTR
CTACTCACAATAGCCCCGGA+CGG	0.678589	5_1:+10005963	Msa0716530:three_prime_UTR
TGTGAGTAGAGCGAGATGCG+TGG	0.699112	5_1:-10005949	None:intergenic
GGAGATCTACATGCTGATGG+TGG	0.705213	5_1:+10005176	Msa0716530:CDS
TATCGACTAGCACCAATGGG+AGG	0.705230	5_1:+10006579	Msa0716530:three_prime_UTR
GGTATGGTGGTGGATCCTTG+AGG	0.722075	5_1:+10005478	Msa0716530:intron
TTCCGAGTTGTAGATCTCAG+CGG	0.734183	5_1:+10005566	Msa0716530:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATAATAAAAAAAGTTTTGT+TGG	+	chr5_1:10005829-10005848	Msa0716530:three_prime_UTR	10.0%
!!	AAAATATTGCAAAATAAATT+AGG	-	chr5_1:10006124-10006143	None:intergenic	10.0%
!!!	TTTTGCAATATTTTTTAATA+AGG	+	chr5_1:10006130-10006149	Msa0716530:intron	10.0%
!!	AAATTAGATTCAATATAATT+TGG	-	chr5_1:10006281-10006300	None:intergenic	10.0%
!!!	AATAATAAAAAAAGTTTTGT+TGG	+	chr5_1:10005829-10005848	Msa0716530:three_prime_UTR	10.0%
!!	AAAATATTGCAAAATAAATT+AGG	-	chr5_1:10006124-10006143	None:intergenic	10.0%
!!!	TTTTGCAATATTTTTTAATA+AGG	+	chr5_1:10006130-10006149	Msa0716530:intron	10.0%
!!	AAATTAGATTCAATATAATT+TGG	-	chr5_1:10006281-10006300	None:intergenic	10.0%
!!	AAAATCCATTATAAAAGAAA+GGG	+	chr5_1:10005015-10005034	Msa0716530:five_prime_UTR	15.0%
!!	TATTAATCTTGAATCAATAA+AGG	-	chr5_1:10005146-10005165	None:intergenic	15.0%
!!	TAAATAAAAAGAATAAGGAA+AGG	-	chr5_1:10005926-10005945	None:intergenic	15.0%
!!	GGTAATAAATAAAAAGAATA+AGG	-	chr5_1:10005931-10005950	None:intergenic	15.0%
!!	AAAAAAAAACATGTAATCAA+GGG	-	chr5_1:10006989-10007008	None:intergenic	15.0%
!!	AAAAAAAAAACATGTAATCA+AGG	-	chr5_1:10006990-10007009	None:intergenic	15.0%
!!!	CATGTTTTTTTTTTTTTTTG+TGG	+	chr5_1:10006996-10007015	Msa0716530:three_prime_UTR	15.0%
!!!	TTAGTTTTTAAATTAAGTTC+AGG	+	chr5_1:10007125-10007144	Msa0716530:three_prime_UTR	15.0%
!!	AAAATCCATTATAAAAGAAA+GGG	+	chr5_1:10005015-10005034	Msa0716530:five_prime_UTR	15.0%
!!	TATTAATCTTGAATCAATAA+AGG	-	chr5_1:10005146-10005165	None:intergenic	15.0%
!!	TAAATAAAAAGAATAAGGAA+AGG	-	chr5_1:10005926-10005945	None:intergenic	15.0%
!!	GGTAATAAATAAAAAGAATA+AGG	-	chr5_1:10005931-10005950	None:intergenic	15.0%
!!	AAAAAAAAACATGTAATCAA+GGG	-	chr5_1:10006989-10007008	None:intergenic	15.0%
!!	AAAAAAAAAACATGTAATCA+AGG	-	chr5_1:10006990-10007009	None:intergenic	15.0%
!!!	CATGTTTTTTTTTTTTTTTG+TGG	+	chr5_1:10006996-10007015	Msa0716530:three_prime_UTR	15.0%
!!!	TTAGTTTTTAAATTAAGTTC+AGG	+	chr5_1:10007125-10007144	Msa0716530:three_prime_UTR	15.0%
!!!	CTTTCTTTTATAATGGATTT+TGG	-	chr5_1:10005016-10005035	None:intergenic	20.0%
!!	CAAAATCCATTATAAAAGAA+AGG	+	chr5_1:10005014-10005033	Msa0716530:five_prime_UTR	20.0%
!!	TATGTTTATATGATGAAGAT+TGG	+	chr5_1:10005652-10005671	Msa0716530:three_prime_UTR	20.0%
!!!	TATTTTTTAATAAGGAATCG+TGG	+	chr5_1:10006138-10006157	Msa0716530:intron	20.0%
!!	CATTAATGTGATTAATACTA+AGG	+	chr5_1:10006666-10006685	Msa0716530:three_prime_UTR	20.0%
!!!	TAGTTTGATTGATTTACTAA+TGG	+	chr5_1:10007218-10007237	Msa0716530:three_prime_UTR	20.0%
!!!	CTTTCTTTTATAATGGATTT+TGG	-	chr5_1:10005016-10005035	None:intergenic	20.0%
!!	CAAAATCCATTATAAAAGAA+AGG	+	chr5_1:10005014-10005033	Msa0716530:five_prime_UTR	20.0%
!!	TATGTTTATATGATGAAGAT+TGG	+	chr5_1:10005652-10005671	Msa0716530:three_prime_UTR	20.0%
!!!	TATTTTTTAATAAGGAATCG+TGG	+	chr5_1:10006138-10006157	Msa0716530:intron	20.0%
!!	CATTAATGTGATTAATACTA+AGG	+	chr5_1:10006666-10006685	Msa0716530:three_prime_UTR	20.0%
!!!	TAGTTTGATTGATTTACTAA+TGG	+	chr5_1:10007218-10007237	Msa0716530:three_prime_UTR	20.0%
!!!	TATAATGGATTTTGGTAGAA+TGG	-	chr5_1:10005008-10005027	None:intergenic	25.0%
!	AATCTTGAATCAATAAAGGT+TGG	-	chr5_1:10005142-10005161	None:intergenic	25.0%
!	AAAGAAATATGATCGAAAAC+AGG	-	chr5_1:10005218-10005237	None:intergenic	25.0%
!	TTTATATGATGAAGATTGGA+AGG	+	chr5_1:10005656-10005675	Msa0716530:three_prime_UTR	25.0%
!	TACATGATCTACTAATTTCT+TGG	-	chr5_1:10006481-10006500	None:intergenic	25.0%
!	AAATTAGTAGATCATGTATC+AGG	+	chr5_1:10006483-10006502	Msa0716530:three_prime_UTR	25.0%
!	AATCACATTAATGATTGAAG+AGG	-	chr5_1:10006659-10006678	None:intergenic	25.0%
!	TTCTTTATTCATACTGTGTA+TGG	+	chr5_1:10006957-10006976	Msa0716530:three_prime_UTR	25.0%
!	TTTGTTCAAAATGCAAATAC+AGG	-	chr5_1:10007086-10007105	None:intergenic	25.0%
!!	ATTTTGAACAAATACACAAG+CGG	+	chr5_1:10007094-10007113	Msa0716530:three_prime_UTR	25.0%
!!!	TATAATGGATTTTGGTAGAA+TGG	-	chr5_1:10005008-10005027	None:intergenic	25.0%
!	AATCTTGAATCAATAAAGGT+TGG	-	chr5_1:10005142-10005161	None:intergenic	25.0%
!	AAAGAAATATGATCGAAAAC+AGG	-	chr5_1:10005218-10005237	None:intergenic	25.0%
!	TTTATATGATGAAGATTGGA+AGG	+	chr5_1:10005656-10005675	Msa0716530:three_prime_UTR	25.0%
!	TACATGATCTACTAATTTCT+TGG	-	chr5_1:10006481-10006500	None:intergenic	25.0%
!	AAATTAGTAGATCATGTATC+AGG	+	chr5_1:10006483-10006502	Msa0716530:three_prime_UTR	25.0%
!	AATCACATTAATGATTGAAG+AGG	-	chr5_1:10006659-10006678	None:intergenic	25.0%
!	TTCTTTATTCATACTGTGTA+TGG	+	chr5_1:10006957-10006976	Msa0716530:three_prime_UTR	25.0%
!	TTTGTTCAAAATGCAAATAC+AGG	-	chr5_1:10007086-10007105	None:intergenic	25.0%
!!	ATTTTGAACAAATACACAAG+CGG	+	chr5_1:10007094-10007113	Msa0716530:three_prime_UTR	25.0%
!	CACAACCCTTTCTTTTATAA+TGG	-	chr5_1:10005023-10005042	None:intergenic	30.0%
	TTGAATCAATAAAGGTTGGA+AGG	-	chr5_1:10005138-10005157	None:intergenic	30.0%
	CAAGATTAATATAGATCCGA+TGG	+	chr5_1:10005155-10005174	Msa0716530:exon	30.0%
	CGAATGAATTATCTACGAAA+CGG	-	chr5_1:10005420-10005439	None:intergenic	30.0%
!	ATTCGATTTTTCCTAAGTCA+AGG	+	chr5_1:10005435-10005454	Msa0716530:intron	30.0%
	TGGATGTAGGATATAACTTT+AGG	+	chr5_1:10005699-10005718	Msa0716530:three_prime_UTR	30.0%
	AAAGAATAAGGAAAGGAACA+AGG	-	chr5_1:10005919-10005938	None:intergenic	30.0%
!	TCTAAAGCAGAAGTTGTAAA+CGG	+	chr5_1:10006330-10006349	Msa0716530:intron	30.0%
	TGGTACACTATCATTCTTAA+TGG	+	chr5_1:10006367-10006386	Msa0716530:intron	30.0%
	GACAAGCATTTATGAACAAA+TGG	-	chr5_1:10006396-10006415	None:intergenic	30.0%
	TGCTTGTCAAATGTAAACTA+TGG	+	chr5_1:10006408-10006427	Msa0716530:intron	30.0%
!	ATAGCTCTGTAAATTCATCA+AGG	+	chr5_1:10006509-10006528	Msa0716530:three_prime_UTR	30.0%
!	TAGCTCTGTAAATTCATCAA+GGG	+	chr5_1:10006510-10006529	Msa0716530:three_prime_UTR	30.0%
	ACATTAATGATTGAAGAGGA+TGG	-	chr5_1:10006655-10006674	None:intergenic	30.0%
!!!	ATGACTAGGTTTTTTTCACT+TGG	+	chr5_1:10006850-10006869	Msa0716530:three_prime_UTR	30.0%
!!	TTTTCACTTGGTTCAAGTTA+AGG	+	chr5_1:10006862-10006881	Msa0716530:three_prime_UTR	30.0%
	AAAAACATGTAATCAAGGGA+GGG	-	chr5_1:10006985-10007004	None:intergenic	30.0%
	AAAAAACATGTAATCAAGGG+AGG	-	chr5_1:10006986-10007005	None:intergenic	30.0%
!!	TTTTTGTGGATTGAAGTTTG+AGG	+	chr5_1:10007010-10007029	Msa0716530:three_prime_UTR	30.0%
!!	TTTTGTGGATTGAAGTTTGA+GGG	+	chr5_1:10007011-10007030	Msa0716530:three_prime_UTR	30.0%
!	CACAACCCTTTCTTTTATAA+TGG	-	chr5_1:10005023-10005042	None:intergenic	30.0%
	TTGAATCAATAAAGGTTGGA+AGG	-	chr5_1:10005138-10005157	None:intergenic	30.0%
	CAAGATTAATATAGATCCGA+TGG	+	chr5_1:10005155-10005174	Msa0716530:exon	30.0%
	CGAATGAATTATCTACGAAA+CGG	-	chr5_1:10005420-10005439	None:intergenic	30.0%
!	ATTCGATTTTTCCTAAGTCA+AGG	+	chr5_1:10005435-10005454	Msa0716530:intron	30.0%
	TGGATGTAGGATATAACTTT+AGG	+	chr5_1:10005699-10005718	Msa0716530:three_prime_UTR	30.0%
	AAAGAATAAGGAAAGGAACA+AGG	-	chr5_1:10005919-10005938	None:intergenic	30.0%
!	TCTAAAGCAGAAGTTGTAAA+CGG	+	chr5_1:10006330-10006349	Msa0716530:intron	30.0%
	TGGTACACTATCATTCTTAA+TGG	+	chr5_1:10006367-10006386	Msa0716530:intron	30.0%
	GACAAGCATTTATGAACAAA+TGG	-	chr5_1:10006396-10006415	None:intergenic	30.0%
	TGCTTGTCAAATGTAAACTA+TGG	+	chr5_1:10006408-10006427	Msa0716530:intron	30.0%
!	ATAGCTCTGTAAATTCATCA+AGG	+	chr5_1:10006509-10006528	Msa0716530:three_prime_UTR	30.0%
!	TAGCTCTGTAAATTCATCAA+GGG	+	chr5_1:10006510-10006529	Msa0716530:three_prime_UTR	30.0%
	ACATTAATGATTGAAGAGGA+TGG	-	chr5_1:10006655-10006674	None:intergenic	30.0%
!!!	ATGACTAGGTTTTTTTCACT+TGG	+	chr5_1:10006850-10006869	Msa0716530:three_prime_UTR	30.0%
!!	TTTTCACTTGGTTCAAGTTA+AGG	+	chr5_1:10006862-10006881	Msa0716530:three_prime_UTR	30.0%
	AAAAACATGTAATCAAGGGA+GGG	-	chr5_1:10006985-10007004	None:intergenic	30.0%
	AAAAAACATGTAATCAAGGG+AGG	-	chr5_1:10006986-10007005	None:intergenic	30.0%
!!	TTTTTGTGGATTGAAGTTTG+AGG	+	chr5_1:10007010-10007029	Msa0716530:three_prime_UTR	30.0%
!!	TTTTGTGGATTGAAGTTTGA+GGG	+	chr5_1:10007011-10007030	Msa0716530:three_prime_UTR	30.0%
!!	ATGGATTTTGGTAGAATGGA+CGG	-	chr5_1:10005004-10005023	None:intergenic	35.0%
	AACGGTTGAACGATATGAAT+TGG	-	chr5_1:10005051-10005070	None:intergenic	35.0%
	ATATCGTTCAACCGTTTCTA+GGG	+	chr5_1:10005055-10005074	Msa0716530:five_prime_UTR	35.0%
	TTGAAGGAAAGAAGGAAGAA+GGG	-	chr5_1:10005091-10005110	None:intergenic	35.0%
	ATTGAAGGAAAGAAGGAAGA+AGG	-	chr5_1:10005092-10005111	None:intergenic	35.0%
!	ATCTGAGATTGAAGGAAAGA+AGG	-	chr5_1:10005099-10005118	None:intergenic	35.0%
	GATTCAGATGTTTCAGATCT+TGG	+	chr5_1:10005619-10005638	Msa0716530:three_prime_UTR	35.0%
	ATGAAGATTGGAAGGTCTTT+AGG	+	chr5_1:10005664-10005683	Msa0716530:three_prime_UTR	35.0%
	TGAAGATTGGAAGGTCTTTA+GGG	+	chr5_1:10005665-10005684	Msa0716530:three_prime_UTR	35.0%
	AATCTCAAGACTCAGTAACA+AGG	+	chr5_1:10005999-10006018	Msa0716530:three_prime_UTR	35.0%
	CTAAAGCAGAAGTTGTAAAC+GGG	+	chr5_1:10006331-10006350	Msa0716530:intron	35.0%
	TAAAGCAGAAGTTGTAAACG+GGG	+	chr5_1:10006332-10006351	Msa0716530:intron	35.0%
	TGTAAATTCATCAAGGGACT+TGG	+	chr5_1:10006516-10006535	Msa0716530:three_prime_UTR	35.0%
!	ATTTATCGACTAGCACCAAT+GGG	+	chr5_1:10006576-10006595	Msa0716530:three_prime_UTR	35.0%
	AGAGTGAAAAGAAGCAATTC+TGG	+	chr5_1:10006610-10006629	Msa0716530:three_prime_UTR	35.0%
	GAGTGAAAAGAAGCAATTCT+GGG	+	chr5_1:10006611-10006630	Msa0716530:three_prime_UTR	35.0%
	TACTAAGGTGAAGATGACTT+CGG	+	chr5_1:10006681-10006700	Msa0716530:three_prime_UTR	35.0%
	AAAACATGTAATCAAGGGAG+GGG	-	chr5_1:10006984-10007003	None:intergenic	35.0%
!!	CAAATACAGGCTTTATTCTC+GGG	-	chr5_1:10007073-10007092	None:intergenic	35.0%
!!	GCAAATACAGGCTTTATTCT+CGG	-	chr5_1:10007074-10007093	None:intergenic	35.0%
!!	ATGGATTTTGGTAGAATGGA+CGG	-	chr5_1:10005004-10005023	None:intergenic	35.0%
	AACGGTTGAACGATATGAAT+TGG	-	chr5_1:10005051-10005070	None:intergenic	35.0%
	ATATCGTTCAACCGTTTCTA+GGG	+	chr5_1:10005055-10005074	Msa0716530:five_prime_UTR	35.0%
	TTGAAGGAAAGAAGGAAGAA+GGG	-	chr5_1:10005091-10005110	None:intergenic	35.0%
	ATTGAAGGAAAGAAGGAAGA+AGG	-	chr5_1:10005092-10005111	None:intergenic	35.0%
!	ATCTGAGATTGAAGGAAAGA+AGG	-	chr5_1:10005099-10005118	None:intergenic	35.0%
	GATTCAGATGTTTCAGATCT+TGG	+	chr5_1:10005619-10005638	Msa0716530:three_prime_UTR	35.0%
	ATGAAGATTGGAAGGTCTTT+AGG	+	chr5_1:10005664-10005683	Msa0716530:three_prime_UTR	35.0%
	TGAAGATTGGAAGGTCTTTA+GGG	+	chr5_1:10005665-10005684	Msa0716530:three_prime_UTR	35.0%
	AATCTCAAGACTCAGTAACA+AGG	+	chr5_1:10005999-10006018	Msa0716530:three_prime_UTR	35.0%
	CTAAAGCAGAAGTTGTAAAC+GGG	+	chr5_1:10006331-10006350	Msa0716530:intron	35.0%
	TAAAGCAGAAGTTGTAAACG+GGG	+	chr5_1:10006332-10006351	Msa0716530:intron	35.0%
	TGTAAATTCATCAAGGGACT+TGG	+	chr5_1:10006516-10006535	Msa0716530:three_prime_UTR	35.0%
!	ATTTATCGACTAGCACCAAT+GGG	+	chr5_1:10006576-10006595	Msa0716530:three_prime_UTR	35.0%
	AGAGTGAAAAGAAGCAATTC+TGG	+	chr5_1:10006610-10006629	Msa0716530:three_prime_UTR	35.0%
	GAGTGAAAAGAAGCAATTCT+GGG	+	chr5_1:10006611-10006630	Msa0716530:three_prime_UTR	35.0%
	TACTAAGGTGAAGATGACTT+CGG	+	chr5_1:10006681-10006700	Msa0716530:three_prime_UTR	35.0%
	AAAACATGTAATCAAGGGAG+GGG	-	chr5_1:10006984-10007003	None:intergenic	35.0%
!!	CAAATACAGGCTTTATTCTC+GGG	-	chr5_1:10007073-10007092	None:intergenic	35.0%
!!	GCAAATACAGGCTTTATTCT+CGG	-	chr5_1:10007074-10007093	None:intergenic	35.0%
!	GCTGAGATTAGTTGTGTTGA+CGG	-	chr5_1:10004982-10005001	None:intergenic	40.0%
	CATATCGTTCAACCGTTTCT+AGG	+	chr5_1:10005054-10005073	Msa0716530:five_prime_UTR	40.0%
	GAATCGTGAAACCCTAGAAA+CGG	-	chr5_1:10005069-10005088	None:intergenic	40.0%
!	TAGACGAGATCTGAGATTGA+AGG	-	chr5_1:10005107-10005126	None:intergenic	40.0%
	ATCAGCATGTAGATCTCCAT+CGG	-	chr5_1:10005174-10005193	None:intergenic	40.0%
!	AACTTGTTCGTCTTGGTTTC+TGG	+	chr5_1:10005392-10005411	Msa0716530:intron	40.0%
!	TTTTTCCTAAGTCAAGGCTC+TGG	+	chr5_1:10005441-10005460	Msa0716530:intron	40.0%
!!!	GGAGGTATTTTTTGCAGGTA+TGG	+	chr5_1:10005462-10005481	Msa0716530:intron	40.0%
!!!	GGTATTTTTTGCAGGTATGG+TGG	+	chr5_1:10005465-10005484	Msa0716530:intron	40.0%
!!!	ATTTTTTGCAGGTATGGTGG+TGG	+	chr5_1:10005468-10005487	Msa0716530:intron	40.0%
!	GTAGATCCGGTGTTGAAATA+TGG	+	chr5_1:10005506-10005525	Msa0716530:CDS	40.0%
	GAATCGCCATATTTCAACAC+CGG	-	chr5_1:10005515-10005534	None:intergenic	40.0%
	TAGATCTCAGCGGTGAATTA+TGG	+	chr5_1:10005576-10005595	Msa0716530:three_prime_UTR	40.0%
	GAAGATTGGAAGGTCTTTAG+GGG	+	chr5_1:10005666-10005685	Msa0716530:three_prime_UTR	40.0%
!	TCTTTAGGGGTGGGATATAA+TGG	+	chr5_1:10005679-10005698	Msa0716530:three_prime_UTR	40.0%
	ATAAGGAAAGGAACAAGGCA+AGG	-	chr5_1:10005914-10005933	None:intergenic	40.0%
	AAACGGGGATTGCTTATTCT+TGG	+	chr5_1:10006347-10006366	Msa0716530:intron	40.0%
	GTAAACTATGGTTTCCGCTT+TGG	+	chr5_1:10006420-10006439	Msa0716530:intron	40.0%
	ACAAAGTACAACAGCCAAAG+CGG	-	chr5_1:10006437-10006456	None:intergenic	40.0%
!	GATTTATCGACTAGCACCAA+TGG	+	chr5_1:10006575-10006594	Msa0716530:three_prime_UTR	40.0%
	ACTAAGGTGAAGATGACTTC+GGG	+	chr5_1:10006682-10006701	Msa0716530:three_prime_UTR	40.0%
!	GCTGAGATTAGTTGTGTTGA+CGG	-	chr5_1:10004982-10005001	None:intergenic	40.0%
	CATATCGTTCAACCGTTTCT+AGG	+	chr5_1:10005054-10005073	Msa0716530:five_prime_UTR	40.0%
	GAATCGTGAAACCCTAGAAA+CGG	-	chr5_1:10005069-10005088	None:intergenic	40.0%
!	TAGACGAGATCTGAGATTGA+AGG	-	chr5_1:10005107-10005126	None:intergenic	40.0%
	ATCAGCATGTAGATCTCCAT+CGG	-	chr5_1:10005174-10005193	None:intergenic	40.0%
!	AACTTGTTCGTCTTGGTTTC+TGG	+	chr5_1:10005392-10005411	Msa0716530:intron	40.0%
!	TTTTTCCTAAGTCAAGGCTC+TGG	+	chr5_1:10005441-10005460	Msa0716530:intron	40.0%
!!!	GGAGGTATTTTTTGCAGGTA+TGG	+	chr5_1:10005462-10005481	Msa0716530:intron	40.0%
!!!	GGTATTTTTTGCAGGTATGG+TGG	+	chr5_1:10005465-10005484	Msa0716530:intron	40.0%
!!!	ATTTTTTGCAGGTATGGTGG+TGG	+	chr5_1:10005468-10005487	Msa0716530:intron	40.0%
!	GTAGATCCGGTGTTGAAATA+TGG	+	chr5_1:10005506-10005525	Msa0716530:CDS	40.0%
	GAATCGCCATATTTCAACAC+CGG	-	chr5_1:10005515-10005534	None:intergenic	40.0%
	TAGATCTCAGCGGTGAATTA+TGG	+	chr5_1:10005576-10005595	Msa0716530:three_prime_UTR	40.0%
	GAAGATTGGAAGGTCTTTAG+GGG	+	chr5_1:10005666-10005685	Msa0716530:three_prime_UTR	40.0%
!	TCTTTAGGGGTGGGATATAA+TGG	+	chr5_1:10005679-10005698	Msa0716530:three_prime_UTR	40.0%
	ATAAGGAAAGGAACAAGGCA+AGG	-	chr5_1:10005914-10005933	None:intergenic	40.0%
	AAACGGGGATTGCTTATTCT+TGG	+	chr5_1:10006347-10006366	Msa0716530:intron	40.0%
	GTAAACTATGGTTTCCGCTT+TGG	+	chr5_1:10006420-10006439	Msa0716530:intron	40.0%
	ACAAAGTACAACAGCCAAAG+CGG	-	chr5_1:10006437-10006456	None:intergenic	40.0%
!	GATTTATCGACTAGCACCAA+TGG	+	chr5_1:10006575-10006594	Msa0716530:three_prime_UTR	40.0%
	ACTAAGGTGAAGATGACTTC+GGG	+	chr5_1:10006682-10006701	Msa0716530:three_prime_UTR	40.0%
!	GATTAGTTGTGTTGACGGTG+TGG	-	chr5_1:10004977-10004996	None:intergenic	45.0%
	GATGGAGATCTACATGCTGA+TGG	+	chr5_1:10005173-10005192	Msa0716530:exon	45.0%
!!!	TGCTGATGGTGGTTTTGTTC+AGG	+	chr5_1:10005187-10005206	Msa0716530:CDS	45.0%
!	AGGTGTGAACTTGTTCGTCT+TGG	+	chr5_1:10005385-10005404	Msa0716530:CDS	45.0%
!!!	GCTCTGGAGGTATTTTTTGC+AGG	+	chr5_1:10005457-10005476	Msa0716530:intron	45.0%
	TTCCGAGTTGTAGATCTCAG+CGG	+	chr5_1:10005566-10005585	Msa0716530:three_prime_UTR	45.0%
!	ATTGGAAGGTCTTTAGGGGT+GGG	+	chr5_1:10005670-10005689	Msa0716530:three_prime_UTR	45.0%
!!	GGGTGGGATATAATGGATGT+AGG	+	chr5_1:10005686-10005705	Msa0716530:three_prime_UTR	45.0%
!!!	TGGTTTTGAGAGAGATCGCA+CGG	+	chr5_1:10005849-10005868	Msa0716530:three_prime_UTR	45.0%
	TTAGCTTCGATGAATCCGTC+CGG	-	chr5_1:10005981-10006000	None:intergenic	45.0%
	GTCATCCTCTTCATCCATTG+AGG	+	chr5_1:10006822-10006841	Msa0716530:three_prime_UTR	45.0%
	CTTCTCCTCAATGGATGAAG+AGG	-	chr5_1:10006830-10006849	None:intergenic	45.0%
	CCTAGTCATCTTCTCCTCAA+TGG	-	chr5_1:10006839-10006858	None:intergenic	45.0%
	CCATTGAGGAGAAGATGACT+AGG	+	chr5_1:10006836-10006855	Msa0716530:three_prime_UTR	45.0%
!	GATTAGTTGTGTTGACGGTG+TGG	-	chr5_1:10004977-10004996	None:intergenic	45.0%
	GATGGAGATCTACATGCTGA+TGG	+	chr5_1:10005173-10005192	Msa0716530:exon	45.0%
!!!	TGCTGATGGTGGTTTTGTTC+AGG	+	chr5_1:10005187-10005206	Msa0716530:CDS	45.0%
!	AGGTGTGAACTTGTTCGTCT+TGG	+	chr5_1:10005385-10005404	Msa0716530:CDS	45.0%
!!!	GCTCTGGAGGTATTTTTTGC+AGG	+	chr5_1:10005457-10005476	Msa0716530:intron	45.0%
	TTCCGAGTTGTAGATCTCAG+CGG	+	chr5_1:10005566-10005585	Msa0716530:three_prime_UTR	45.0%
!	ATTGGAAGGTCTTTAGGGGT+GGG	+	chr5_1:10005670-10005689	Msa0716530:three_prime_UTR	45.0%
!!	GGGTGGGATATAATGGATGT+AGG	+	chr5_1:10005686-10005705	Msa0716530:three_prime_UTR	45.0%
!!!	TGGTTTTGAGAGAGATCGCA+CGG	+	chr5_1:10005849-10005868	Msa0716530:three_prime_UTR	45.0%
	TTAGCTTCGATGAATCCGTC+CGG	-	chr5_1:10005981-10006000	None:intergenic	45.0%
	GTCATCCTCTTCATCCATTG+AGG	+	chr5_1:10006822-10006841	Msa0716530:three_prime_UTR	45.0%
	CTTCTCCTCAATGGATGAAG+AGG	-	chr5_1:10006830-10006849	None:intergenic	45.0%
	CCTAGTCATCTTCTCCTCAA+TGG	-	chr5_1:10006839-10006858	None:intergenic	45.0%
	CCATTGAGGAGAAGATGACT+AGG	+	chr5_1:10006836-10006855	Msa0716530:three_prime_UTR	45.0%
	GGAGATCTACATGCTGATGG+TGG	+	chr5_1:10005176-10005195	Msa0716530:CDS	50.0%
	GCATCAGATCTACGATCCAG+AGG	+	chr5_1:10005365-10005384	Msa0716530:CDS	50.0%
	GACGAACAAGTTCACACCTC+TGG	-	chr5_1:10005384-10005403	None:intergenic	50.0%
!	TTCCTAAGTCAAGGCTCTGG+AGG	+	chr5_1:10005444-10005463	Msa0716530:intron	50.0%
	TACCTCCAGAGCCTTGACTT+AGG	-	chr5_1:10005449-10005468	None:intergenic	50.0%
	CCTTGAGGAGATCGTAGATC+CGG	+	chr5_1:10005493-10005512	Msa0716530:CDS	50.0%
	CACCGCTGAGATCTACAACT+CGG	-	chr5_1:10005571-10005590	None:intergenic	50.0%
!	GATTGGAAGGTCTTTAGGGG+TGG	+	chr5_1:10005669-10005688	Msa0716530:three_prime_UTR	50.0%
	TTCATACGCTCAGCGCATCA+AGG	-	chr5_1:10005734-10005753	None:intergenic	50.0%
	TTGATGCGCTGAGCGTATGA+AGG	+	chr5_1:10005733-10005752	Msa0716530:three_prime_UTR	50.0%
	CGGATGACAAGATCACTCTC+AGG	-	chr5_1:10005766-10005785	None:intergenic	50.0%
!!!	GGTTTTGAGAGAGATCGCAC+GGG	+	chr5_1:10005850-10005869	Msa0716530:three_prime_UTR	50.0%
!!!	GTTTTGAGAGAGATCGCACG+GGG	+	chr5_1:10005851-10005870	Msa0716530:three_prime_UTR	50.0%
	ACACACACGAGAGAAGATCC+CGG	-	chr5_1:10005884-10005903	None:intergenic	50.0%
	TAGCTTCGATGAATCCGTCC+GGG	-	chr5_1:10005980-10005999	None:intergenic	50.0%
!	TATCGACTAGCACCAATGGG+AGG	+	chr5_1:10006579-10006598	Msa0716530:three_prime_UTR	50.0%
	ACTAGCACCAATGGGAGGAA+TGG	+	chr5_1:10006584-10006603	Msa0716530:three_prime_UTR	50.0%
	CTCTCTTCCATTCCTCCCAT+TGG	-	chr5_1:10006594-10006613	None:intergenic	50.0%
	GGAGATCTACATGCTGATGG+TGG	+	chr5_1:10005176-10005195	Msa0716530:CDS	50.0%
	GCATCAGATCTACGATCCAG+AGG	+	chr5_1:10005365-10005384	Msa0716530:CDS	50.0%
	GACGAACAAGTTCACACCTC+TGG	-	chr5_1:10005384-10005403	None:intergenic	50.0%
!	TTCCTAAGTCAAGGCTCTGG+AGG	+	chr5_1:10005444-10005463	Msa0716530:intron	50.0%
	TACCTCCAGAGCCTTGACTT+AGG	-	chr5_1:10005449-10005468	None:intergenic	50.0%
	CCTTGAGGAGATCGTAGATC+CGG	+	chr5_1:10005493-10005512	Msa0716530:CDS	50.0%
	CACCGCTGAGATCTACAACT+CGG	-	chr5_1:10005571-10005590	None:intergenic	50.0%
!	GATTGGAAGGTCTTTAGGGG+TGG	+	chr5_1:10005669-10005688	Msa0716530:three_prime_UTR	50.0%
	TTCATACGCTCAGCGCATCA+AGG	-	chr5_1:10005734-10005753	None:intergenic	50.0%
	TTGATGCGCTGAGCGTATGA+AGG	+	chr5_1:10005733-10005752	Msa0716530:three_prime_UTR	50.0%
	CGGATGACAAGATCACTCTC+AGG	-	chr5_1:10005766-10005785	None:intergenic	50.0%
!!!	GGTTTTGAGAGAGATCGCAC+GGG	+	chr5_1:10005850-10005869	Msa0716530:three_prime_UTR	50.0%
!!!	GTTTTGAGAGAGATCGCACG+GGG	+	chr5_1:10005851-10005870	Msa0716530:three_prime_UTR	50.0%
	ACACACACGAGAGAAGATCC+CGG	-	chr5_1:10005884-10005903	None:intergenic	50.0%
	TAGCTTCGATGAATCCGTCC+GGG	-	chr5_1:10005980-10005999	None:intergenic	50.0%
!	TATCGACTAGCACCAATGGG+AGG	+	chr5_1:10006579-10006598	Msa0716530:three_prime_UTR	50.0%
	ACTAGCACCAATGGGAGGAA+TGG	+	chr5_1:10006584-10006603	Msa0716530:three_prime_UTR	50.0%
	CTCTCTTCCATTCCTCCCAT+TGG	-	chr5_1:10006594-10006613	None:intergenic	50.0%
!!	GGTATGGTGGTGGATCCTTG+AGG	+	chr5_1:10005478-10005497	Msa0716530:intron	55.0%
	CCGGATCTACGATCTCCTCA+AGG	-	chr5_1:10005496-10005515	None:intergenic	55.0%
	CATCCGACCTTTGCCATGTC+AGG	+	chr5_1:10005780-10005799	Msa0716530:three_prime_UTR	55.0%
	GCACCTGACATGGCAAAGGT+CGG	-	chr5_1:10005786-10005805	None:intergenic	55.0%
	AAGCGCACCTGACATGGCAA+AGG	-	chr5_1:10005790-10005809	None:intergenic	55.0%
	CACACACGAGAGAAGATCCC+GGG	-	chr5_1:10005883-10005902	None:intergenic	55.0%
	GGGATCTTCTCTCGTGTGTG+TGG	+	chr5_1:10005883-10005902	Msa0716530:three_prime_UTR	55.0%
	TGTGAGTAGAGCGAGATGCG+TGG	-	chr5_1:10005952-10005971	None:intergenic	55.0%
	CGCTCTACTCACAATAGCCC+CGG	+	chr5_1:10005959-10005978	Msa0716530:three_prime_UTR	55.0%
	CTACTCACAATAGCCCCGGA+CGG	+	chr5_1:10005963-10005982	Msa0716530:three_prime_UTR	55.0%
	AGCTTCGATGAATCCGTCCG+GGG	-	chr5_1:10005979-10005998	None:intergenic	55.0%
!!	GGTATGGTGGTGGATCCTTG+AGG	+	chr5_1:10005478-10005497	Msa0716530:intron	55.0%
	CCGGATCTACGATCTCCTCA+AGG	-	chr5_1:10005496-10005515	None:intergenic	55.0%
	CATCCGACCTTTGCCATGTC+AGG	+	chr5_1:10005780-10005799	Msa0716530:three_prime_UTR	55.0%
	GCACCTGACATGGCAAAGGT+CGG	-	chr5_1:10005786-10005805	None:intergenic	55.0%
	AAGCGCACCTGACATGGCAA+AGG	-	chr5_1:10005790-10005809	None:intergenic	55.0%
	CACACACGAGAGAAGATCCC+GGG	-	chr5_1:10005883-10005902	None:intergenic	55.0%
	GGGATCTTCTCTCGTGTGTG+TGG	+	chr5_1:10005883-10005902	Msa0716530:three_prime_UTR	55.0%
	TGTGAGTAGAGCGAGATGCG+TGG	-	chr5_1:10005952-10005971	None:intergenic	55.0%
	CGCTCTACTCACAATAGCCC+CGG	+	chr5_1:10005959-10005978	Msa0716530:three_prime_UTR	55.0%
	CTACTCACAATAGCCCCGGA+CGG	+	chr5_1:10005963-10005982	Msa0716530:three_prime_UTR	55.0%
	AGCTTCGATGAATCCGTCCG+GGG	-	chr5_1:10005979-10005998	None:intergenic	55.0%
	CCAAGCAAGCGCACCTGACA+TGG	-	chr5_1:10005796-10005815	None:intergenic	60.0%
	CCATGTCAGGTGCGCTTGCT+TGG	+	chr5_1:10005793-10005812	Msa0716530:three_prime_UTR	60.0%
!	GATCGCACGGGGTTTTTGCC+CGG	+	chr5_1:10005862-10005881	Msa0716530:three_prime_UTR	60.0%
!	ATCGCACGGGGTTTTTGCCC+GGG	+	chr5_1:10005863-10005882	Msa0716530:three_prime_UTR	60.0%
	CCAAGCAAGCGCACCTGACA+TGG	-	chr5_1:10005796-10005815	None:intergenic	60.0%
	CCATGTCAGGTGCGCTTGCT+TGG	+	chr5_1:10005793-10005812	Msa0716530:three_prime_UTR	60.0%
!	GATCGCACGGGGTTTTTGCC+CGG	+	chr5_1:10005862-10005881	Msa0716530:three_prime_UTR	60.0%
!	ATCGCACGGGGTTTTTGCCC+GGG	+	chr5_1:10005863-10005882	Msa0716530:three_prime_UTR	60.0%
	GTCAGGTGCGCTTGCTTGGC+AGG	+	chr5_1:10005797-10005816	Msa0716530:three_prime_UTR	65.0%
	GTCAGGTGCGCTTGCTTGGC+AGG	+	chr5_1:10005797-10005816	Msa0716530:three_prime_UTR	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	10004973	10007250	10004973	ID=Msa0716530;Name=Msa0716530
chr5_1	mRNA	10004973	10007250	10004973	ID=Msa0716530-mRNA-1;Parent=Msa0716530;Name=Msa0716530-mRNA-1;_AED=0.12;_eAED=0.12;_QI=201\|0.5\|0.6\|1\|0.25\|0.4\|5\|1250\|62
chr5_1	exon	10004973	10005208	10004973	ID=Msa0716530-mRNA-1:exon:4013;Parent=Msa0716530-mRNA-1
chr5_1	exon	10005340	10005406	10005340	ID=Msa0716530-mRNA-1:exon:4014;Parent=Msa0716530-mRNA-1
chr5_1	exon	10005457	10005465	10005457	ID=Msa0716530-mRNA-1:exon:4015;Parent=Msa0716530-mRNA-1
chr5_1	exon	10005479	10006020	10005479	ID=Msa0716530-mRNA-1:exon:4016;Parent=Msa0716530-mRNA-1
chr5_1	exon	10006465	10007250	10006465	ID=Msa0716530-mRNA-1:exon:4017;Parent=Msa0716530-mRNA-1
chr5_1	five_prime_UTR	10004973	10005173	10004973	ID=Msa0716530-mRNA-1:five_prime_utr;Parent=Msa0716530-mRNA-1
chr5_1	CDS	10005174	10005208	10005174	ID=Msa0716530-mRNA-1:cds;Parent=Msa0716530-mRNA-1
chr5_1	CDS	10005340	10005406	10005340	ID=Msa0716530-mRNA-1:cds;Parent=Msa0716530-mRNA-1
chr5_1	CDS	10005457	10005465	10005457	ID=Msa0716530-mRNA-1:cds;Parent=Msa0716530-mRNA-1
chr5_1	CDS	10005479	10005556	10005479	ID=Msa0716530-mRNA-1:cds;Parent=Msa0716530-mRNA-1
chr5_1	three_prime_UTR	10005557	10006020	10005557	ID=Msa0716530-mRNA-1:three_prime_utr;Parent=Msa0716530-mRNA-1
chr5_1	three_prime_UTR	10006465	10007250	10006465	ID=Msa0716530-mRNA-1:three_prime_utr;Parent=Msa0716530-mRNA-1

Gene Sequence	>Msa0716530 ATGGAGATCTACATGCTGATGGTGGTTTTGTTCAGAAATTTCATAGATCTGAATCTTCGTGCATCAGATCTACGATCCAGAGGTGTGAACTTGTTCGTCTTGGCTCTGGAGGTATGGTGGTGGATCCTTGAGGAGATCGTAGATCCGGTGTTGAAATATGGCGATTCAACTGTTGATGATGATTATTGA
Protein sequence	>Msa0716530 MEIYMLMVVLFRNFIDLNLRASDLRSRGVNLFVLALEVWWWILEEIVDPVLKYGDSTVDDDY*