Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1098430 | XP_013447616.1 | 90.909 | 110 | 4 | 2 | 1 | 110 | 1 | 104 | 3.89e-59 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1098430 | A0A072TW64 | 90.909 | 110 | 4 | 2 | 1 | 110 | 1 | 104 | 1.86e-59 | 192 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023330 | Msa1098430 | 0.811931 | 5.468729e-51 | -8.615850e-47 |
| Msa0031180 | Msa1098430 | 0.806761 | 7.030556e-50 | -8.615850e-47 |
| Msa0042550 | Msa1098430 | 0.848652 | 5.348834e-60 | -8.615850e-47 |
| Msa0058600 | Msa1098430 | 0.817492 | 3.206393e-52 | -8.615850e-47 |
| Msa0078480 | Msa1098430 | 0.804543 | 2.053929e-49 | -8.615850e-47 |
| Msa0099620 | Msa1098430 | 0.802683 | 4.994501e-49 | -8.615850e-47 |
| Msa0099650 | Msa1098430 | 0.800768 | 1.234095e-48 | -8.615850e-47 |
| Msa0100090 | Msa1098430 | 0.801562 | 8.492324e-49 | -8.615850e-47 |
| Msa0610670 | Msa1098430 | 0.823813 | 1.131372e-53 | -8.615850e-47 |
| Msa0615220 | Msa1098430 | 0.824919 | 6.218093e-54 | -8.615850e-47 |
| Msa0624960 | Msa1098430 | 0.815540 | 8.774828e-52 | -8.615850e-47 |
| Msa0626990 | Msa1098430 | 0.814315 | 1.640133e-51 | -8.615850e-47 |
| Msa0627350 | Msa1098430 | 0.816531 | 5.271376e-52 | -8.615850e-47 |
| Msa0651270 | Msa1098430 | 0.833810 | 4.305693e-56 | -8.615850e-47 |
| Msa0667740 | Msa1098430 | 0.825904 | 3.633492e-54 | -8.615850e-47 |
| Msa0679550 | Msa1098430 | 0.801198 | 1.008460e-48 | -8.615850e-47 |
| Msa0715410 | Msa1098430 | 0.816957 | 4.229156e-52 | -8.615850e-47 |
| Msa0738030 | Msa1098430 | 0.820988 | 5.125990e-53 | -8.615850e-47 |
| Msa0749440 | Msa1098430 | 0.811604 | 6.443754e-51 | -8.615850e-47 |
| Msa0751850 | Msa1098430 | 0.815543 | 8.758574e-52 | -8.615850e-47 |
| Msa0763890 | Msa1098430 | 0.809791 | 1.588946e-50 | -8.615850e-47 |
| Msa0763920 | Msa1098430 | 0.810690 | 1.016769e-50 | -8.615850e-47 |
| Msa0766090 | Msa1098430 | 0.813333 | 2.699233e-51 | -8.615850e-47 |
| Msa0780280 | Msa1098430 | 0.806302 | 8.788783e-50 | -8.615850e-47 |
| Msa0817460 | Msa1098430 | 0.824586 | 7.448092e-54 | -8.615850e-47 |
| Msa0821800 | Msa1098430 | 0.806353 | 8.571914e-50 | -8.615850e-47 |
| Msa0831150 | Msa1098430 | 0.808936 | 2.424623e-50 | -8.615850e-47 |
| Msa0831850 | Msa1098430 | 0.811225 | 7.787945e-51 | -8.615850e-47 |
| Msa0138020 | Msa1098430 | 0.822829 | 1.921726e-53 | -8.615850e-47 |
| Msa0143850 | Msa1098430 | 0.801954 | 7.055955e-49 | -8.615850e-47 |
| Msa0143930 | Msa1098430 | 0.805674 | 1.190766e-49 | -8.615850e-47 |
| Msa0178510 | Msa1098430 | 0.850571 | 1.557632e-60 | -8.615850e-47 |
| Msa0190530 | Msa1098430 | 0.808384 | 3.180316e-50 | -8.615850e-47 |
| Msa0200850 | Msa1098430 | 0.808710 | 2.709710e-50 | -8.615850e-47 |
| Msa0206010 | Msa1098430 | 0.804967 | 1.675276e-49 | -8.615850e-47 |
| Msa0223120 | Msa1098430 | 0.802202 | 6.273729e-49 | -8.615850e-47 |
| Msa0225220 | Msa1098430 | 0.819375 | 1.200603e-52 | -8.615850e-47 |
| Msa0229590 | Msa1098430 | 0.805318 | 1.414212e-49 | -8.615850e-47 |
| Msa0387050 | Msa1098430 | 0.804494 | 2.102993e-49 | -8.615850e-47 |
| Msa0410580 | Msa1098430 | 0.824136 | 9.503936e-54 | -8.615850e-47 |
| Msa0417650 | Msa1098430 | 0.807153 | 5.807608e-50 | -8.615850e-47 |
| Msa0417730 | Msa1098430 | 0.803985 | 2.684571e-49 | -8.615850e-47 |
| Msa0433410 | Msa1098430 | -0.811923 | 5.490500e-51 | -8.615850e-47 |
| Msa0445280 | Msa1098430 | 0.802611 | 5.168137e-49 | -8.615850e-47 |
| Msa0986510 | Msa1098430 | 0.813378 | 2.638474e-51 | -8.615850e-47 |
| Msa1008750 | Msa1098430 | 0.802865 | 4.580133e-49 | -8.615850e-47 |
| Msa1026410 | Msa1098430 | 0.801256 | 9.812523e-49 | -8.615850e-47 |
| Msa1026640 | Msa1098430 | 0.822587 | 2.187336e-53 | -8.615850e-47 |
| Msa1050410 | Msa1098430 | 0.803298 | 3.726240e-49 | -8.615850e-47 |
| Msa1063400 | Msa1098430 | 0.813640 | 2.310874e-51 | -8.615850e-47 |
| Msa1077340 | Msa1098430 | 0.815154 | 1.069148e-51 | -8.615850e-47 |
| Msa1087990 | Msa1098430 | 0.809973 | 1.451851e-50 | -8.615850e-47 |
| Msa1098420 | Msa1098430 | 0.803232 | 3.845816e-49 | -8.615850e-47 |
| Msa1098430 | Msa1115280 | 0.806432 | 8.250178e-50 | -8.615850e-47 |
| Msa1098430 | Msa1205370 | 0.807322 | 5.349755e-50 | -8.615850e-47 |
| Msa1098430 | Msa1236420 | 0.802184 | 6.328791e-49 | -8.615850e-47 |
| Msa1098430 | Msa1250290 | 0.806933 | 6.467106e-50 | -8.615850e-47 |
| Msa1098430 | Msa1270660 | 0.820366 | 7.126049e-53 | -8.615850e-47 |
| Msa1098430 | Msa1270730 | 0.825892 | 3.658915e-54 | -8.615850e-47 |
| Msa1098430 | Msa1276440 | 0.825389 | 4.813558e-54 | -8.615850e-47 |
| Msa1098430 | Msa1299070 | 0.809994 | 1.437069e-50 | -8.615850e-47 |
| Msa1098430 | Msa1307550 | 0.827145 | 1.838437e-54 | -8.615850e-47 |
| Msa1098430 | Msa1323290 | 0.814071 | 1.856380e-51 | -8.615850e-47 |
| Msa1098430 | Msa1333730 | 0.805376 | 1.375351e-49 | -8.615850e-47 |
| Msa1098430 | Msa1335230 | 0.815342 | 9.710544e-52 | -8.615850e-47 |
| Msa1098430 | Msa1335710 | 0.810175 | 1.313364e-50 | -8.615850e-47 |
| Msa1098430 | Msa1349450 | 0.803878 | 2.824973e-49 | -8.615850e-47 |
| Msa1098430 | Msa1371940 | 0.809985 | 1.443259e-50 | -8.615850e-47 |
| Msa1098430 | Msa1372150 | 0.853343 | 2.545071e-61 | -8.615850e-47 |
| Msa1098430 | Msa1377570 | 0.804052 | 2.599405e-49 | -8.615850e-47 |
| Msa1098430 | Msa1423820 | 0.812749 | 3.625397e-51 | -8.615850e-47 |
| Msa1098430 | Msa1462360 | 0.801616 | 8.278245e-49 | -8.615850e-47 |
| Msa0241140 | Msa1098430 | 0.805049 | 1.610527e-49 | -8.615850e-47 |
| Msa0243480 | Msa1098430 | 0.806096 | 9.711804e-50 | -8.615850e-47 |
| Msa0249100 | Msa1098430 | 0.803706 | 3.067807e-49 | -8.615850e-47 |
| Msa0265150 | Msa1098430 | 0.801743 | 7.797144e-49 | -8.615850e-47 |
| Msa0269560 | Msa1098430 | 0.804270 | 2.341747e-49 | -8.615850e-47 |
| Msa0271550 | Msa1098430 | 0.807652 | 4.552157e-50 | -8.615850e-47 |
| Msa0281930 | Msa1098430 | 0.804920 | 1.713210e-49 | -8.615850e-47 |
| Msa0300150 | Msa1098430 | 0.808282 | 3.343658e-50 | -8.615850e-47 |
| Msa0308460 | Msa1098430 | 0.805550 | 1.264362e-49 | -8.615850e-47 |
| Msa0321080 | Msa1098430 | 0.807320 | 5.355592e-50 | -8.615850e-47 |
| Msa0322680 | Msa1098430 | 0.812906 | 3.349844e-51 | -8.615850e-47 |
| Msa0326110 | Msa1098430 | 0.812055 | 5.138577e-51 | -8.615850e-47 |
| Msa0326520 | Msa1098430 | 0.803964 | 2.710867e-49 | -8.615850e-47 |
| Msa0347950 | Msa1098430 | 0.803537 | 3.325071e-49 | -8.615850e-47 |
| Msa0357830 | Msa1098430 | 0.803697 | 3.079705e-49 | -8.615850e-47 |
| Msa0484780 | Msa1098430 | 0.814541 | 1.461922e-51 | -8.615850e-47 |
| Msa0485490 | Msa1098430 | 0.822188 | 2.707162e-53 | -8.615850e-47 |
| Msa0520700 | Msa1098430 | 0.801553 | 8.528436e-49 | -8.615850e-47 |
| Msa0530650 | Msa1098430 | 0.823598 | 1.270559e-53 | -8.615850e-47 |
| Msa0532930 | Msa1098430 | 0.802512 | 5.416666e-49 | -8.615850e-47 |
| Msa0559500 | Msa1098430 | 0.826128 | 3.215036e-54 | -8.615850e-47 |
| Msa0565350 | Msa1098430 | 0.805009 | 1.641569e-49 | -8.615850e-47 |
| Msa0579100 | Msa1098430 | 0.800837 | 1.194907e-48 | -8.615850e-47 |
| Msa0579130 | Msa1098430 | 0.825120 | 5.573238e-54 | -8.615850e-47 |
| Msa0579200 | Msa1098430 | 0.823965 | 1.042339e-53 | -8.615850e-47 |
| Msa0587190 | Msa1098430 | 0.807281 | 5.456314e-50 | -8.615850e-47 |
| Msa0844110 | Msa1098430 | 0.806737 | 7.112301e-50 | -8.615850e-47 |
| Msa0847880 | Msa1098430 | 0.810554 | 1.088093e-50 | -8.615850e-47 |
| Msa0873860 | Msa1098430 | 0.800471 | 1.418814e-48 | -8.615850e-47 |
| Msa0876360 | Msa1098430 | 0.811133 | 8.154601e-51 | -8.615850e-47 |
| Msa0902830 | Msa1098430 | 0.813459 | 2.532894e-51 | -8.615850e-47 |
| Msa0935770 | Msa1098430 | 0.802889 | 4.528652e-49 | -8.615850e-47 |
| Msa0936390 | Msa1098430 | 0.801469 | 8.871161e-49 | -8.615850e-47 |
| Msa0972810 | Msa1098430 | 0.801037 | 1.087816e-48 | -8.615850e-47 |
| Msa0978700 | Msa1098430 | 0.806613 | 7.554283e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1098430 | MtrunA17_Chr7g0219531 | 90.909 | 110 | 4 | 2 | 1 | 110 | 1 | 104 | 3.58e-63 | 192 |
| Msa1098430 | MtrunA17_Chr7g0219521 | 72.650 | 117 | 19 | 3 | 1 | 110 | 1 | 111 | 3.39e-48 | 153 |
| Msa1098430 | MtrunA17_Chr7g0219541 | 72.072 | 111 | 29 | 2 | 1 | 110 | 1 | 110 | 1.24e-43 | 143 |
| Msa1098430 | MtrunA17_Chr7g0219551 | 53.774 | 106 | 44 | 5 | 6 | 109 | 47 | 149 | 2.32e-26 | 99.4 |
| Msa1098430 | MtrunA17_Chr2g0308461 | 47.692 | 65 | 31 | 1 | 49 | 110 | 281 | 345 | 6.42e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1098430 | AT4G26190.2 | 50.820 | 61 | 30 | 0 | 48 | 108 | 841 | 901 | 2.96e-16 | 73.6 |
| Msa1098430 | AT4G26190.5 | 50.820 | 61 | 30 | 0 | 48 | 108 | 841 | 901 | 2.96e-16 | 73.6 |
| Msa1098430 | AT4G26190.3 | 50.820 | 61 | 30 | 0 | 48 | 108 | 841 | 901 | 2.96e-16 | 73.6 |
| Msa1098430 | AT4G26190.1 | 50.820 | 61 | 30 | 0 | 48 | 108 | 841 | 901 | 2.96e-16 | 73.6 |
| Msa1098430 | AT4G26190.4 | 50.820 | 61 | 30 | 0 | 48 | 108 | 841 | 901 | 2.96e-16 | 73.6 |
| Msa1098430 | AT3G29760.4 | 55.319 | 47 | 20 | 1 | 62 | 108 | 318 | 363 | 1.29e-11 | 60.1 |
| Msa1098430 | AT3G29760.1 | 55.319 | 47 | 20 | 1 | 62 | 108 | 318 | 363 | 3.48e-11 | 58.9 |
| Msa1098430 | AT3G29760.5 | 55.319 | 47 | 20 | 1 | 62 | 108 | 318 | 363 | 5.49e-11 | 58.2 |
| Msa1098430 | AT3G29760.3 | 55.319 | 47 | 20 | 1 | 62 | 108 | 318 | 363 | 5.49e-11 | 58.2 |
| Msa1098430 | AT3G29760.2 | 55.319 | 47 | 20 | 1 | 62 | 108 | 318 | 363 | 7.11e-11 | 58.2 |
Find 8 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATTGTTCTTGATTTAAA+TGG | 0.160699 | 7_3:+25706865 | Msa1098430:CDS |
| TTCAGAAACTCGTGATAAAA+AGG | 0.276322 | 7_3:-25707370 | None:intergenic |
| TTCATGGCTATGAATGCATC+TGG | 0.415674 | 7_3:-25706931 | None:intergenic |
| GCATCTGGTTTGACATGCCT+TGG | 0.484849 | 7_3:-25706916 | None:intergenic |
| TCAATTGAAATATTATCAGT+TGG | 0.493002 | 7_3:+25706823 | Msa1098430:CDS |
| CTGCTTTGAGAGATTTGAAG+TGG | 0.522255 | 7_3:+25707396 | Msa1098430:CDS |
| GGTTTGACATGCCTTGGATG+TGG | 0.541762 | 7_3:-25706910 | None:intergenic |
| AGAGATTTGAAGTGGCTGTG+TGG | 0.661167 | 7_3:+25707404 | Msa1098430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAGCATTTAATTTTGAT+AGG | + | chr7_3:25707024-25707043 | Msa1098430:intron | 15.0% |
| !!! | TTAATCTATTTTCTAATGAT+TGG | + | chr7_3:25707315-25707334 | Msa1098430:intron | 15.0% |
| !! | TCAATTGAAATATTATCAGT+TGG | + | chr7_3:25706823-25706842 | Msa1098430:CDS | 20.0% |
| !! | CTTATTGTTCTTGATTTAAA+TGG | + | chr7_3:25706865-25706884 | Msa1098430:CDS | 20.0% |
| !! | TTCAAATTCAAACATAAGAT+TGG | - | chr7_3:25707070-25707089 | None:intergenic | 20.0% |
| ! | TACTGATGAAAAAGAAGAAA+AGG | + | chr7_3:25706780-25706799 | Msa1098430:CDS | 25.0% |
| ! | ATATACACAATCAATGAGTA+AGG | - | chr7_3:25707279-25707298 | None:intergenic | 25.0% |
| !! | CATTGATTGTGTATATGAAT+TGG | + | chr7_3:25707283-25707302 | Msa1098430:intron | 25.0% |
| !! | ATATTGTTTTTCCACATCCA+AGG | + | chr7_3:25706899-25706918 | Msa1098430:CDS | 30.0% |
| TTCAGAAACTCGTGATAAAA+AGG | - | chr7_3:25707373-25707392 | None:intergenic | 30.0% | |
| ! | CTTTTTCTCACATGCTTTCA+TGG | - | chr7_3:25706950-25706969 | None:intergenic | 35.0% |
| TTCATGGCTATGAATGCATC+TGG | - | chr7_3:25706934-25706953 | None:intergenic | 40.0% | |
| TCCCATGTTGTGTCGAAAAT+CGG | + | chr7_3:25707172-25707191 | Msa1098430:intron | 40.0% | |
| ! | TCCGATTTTCGACACAACAT+GGG | - | chr7_3:25707176-25707195 | None:intergenic | 40.0% |
| ACACCTTCAGTTTGGATTGT+CGG | + | chr7_3:25707197-25707216 | Msa1098430:intron | 40.0% | |
| ! | TGTTTTCCTCAGTATTCGAG+AGG | + | chr7_3:25707347-25707366 | Msa1098430:intron | 40.0% |
| !! | CTGCTTTGAGAGATTTGAAG+TGG | + | chr7_3:25707396-25707415 | Msa1098430:CDS | 40.0% |
| ! | GTCCGATTTTCGACACAACA+TGG | - | chr7_3:25707177-25707196 | None:intergenic | 45.0% |
| AATCGGACACACCTTCAGTT+TGG | + | chr7_3:25707189-25707208 | Msa1098430:intron | 45.0% | |
| !! | TTGGATTGTCGGTGCTACAT+AGG | + | chr7_3:25707208-25707227 | Msa1098430:intron | 45.0% |
| AAAAGGCCTCTCGAATACTG+AGG | - | chr7_3:25707356-25707375 | None:intergenic | 45.0% | |
| ! | AGAGATTTGAAGTGGCTGTG+TGG | + | chr7_3:25707404-25707423 | Msa1098430:CDS | 45.0% |
| GGTTTGACATGCCTTGGATG+TGG | - | chr7_3:25706913-25706932 | None:intergenic | 50.0% | |
| ! | GCATCTGGTTTGACATGCCT+TGG | - | chr7_3:25706919-25706938 | None:intergenic | 50.0% |
| ! | GCACCGACAATCCAAACTGA+AGG | - | chr7_3:25707203-25707222 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 25706712 | 25707444 | 25706712 | ID=Msa1098430;Name=Msa1098430 |
| chr7_3 | mRNA | 25706712 | 25707444 | 25706712 | ID=Msa1098430-mRNA-1;Parent=Msa1098430;Name=Msa1098430-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|110 |
| chr7_3 | exon | 25706712 | 25706958 | 25706712 | ID=Msa1098430-mRNA-1:exon:5514;Parent=Msa1098430-mRNA-1 |
| chr7_3 | exon | 25707359 | 25707444 | 25707359 | ID=Msa1098430-mRNA-1:exon:5515;Parent=Msa1098430-mRNA-1 |
| chr7_3 | CDS | 25706712 | 25706958 | 25706712 | ID=Msa1098430-mRNA-1:cds;Parent=Msa1098430-mRNA-1 |
| chr7_3 | CDS | 25707359 | 25707444 | 25707359 | ID=Msa1098430-mRNA-1:cds;Parent=Msa1098430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
