AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1323290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323290 MtrunA17_Chr8g0350281 95.699 186 7 1 1 186 1 185 4.81e-128 357
Msa1323290 MtrunA17_Chr8g0350221 53.933 178 58 6 7 167 5 175 2.70e-52 165
Msa1323290 MtrunA17_Chr8g0350241 53.672 177 61 5 7 168 5 175 5.89e-51 162
Msa1323290 MtrunA17_Chr8g0350231 51.099 182 58 5 7 167 5 176 2.08e-49 158
Msa1323290 MtrunA17_Chr8g0350271 51.412 177 64 5 7 167 5 175 5.05e-49 157
Msa1323290 MtrunA17_Chr3g0109941 51.412 177 65 5 7 168 5 175 9.01e-49 156
Msa1323290 MtrunA17_Chr8g0350291 51.705 176 64 5 7 167 5 174 7.28e-48 154
Msa1323290 MtrunA17_Chr8g0350211 37.500 128 76 3 7 131 2 128 4.73e-20 82.8
Msa1323290 MtrunA17_Chr8g0350191 36.923 130 74 5 7 131 2 128 3.14e-19 80.5
Msa1323290 MtrunA17_Chr8g0350201 36.923 130 74 5 7 131 2 128 4.41e-19 80.1
Msa1323290 MtrunA17_Chr7g0246561 36.471 170 87 9 7 168 2 158 2.07e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCATTGGGATTCCAGTTT+TGG 0.209973 8_4:-31543427 Msa1323290:CDS
TTAGAAATCACCATTATTTC+CGG 0.251148 8_4:-31543885 Msa1323290:CDS
GAGTTATGGGTTGAGGGATT+GGG 0.319102 8_4:-31543580 Msa1323290:CDS
AAAACGAGGGTTTACTTTCA+TGG 0.320231 8_4:-31543764 Msa1323290:CDS
TGAGTTATGGGTTGAGGGAT+TGG 0.329761 8_4:-31543581 Msa1323290:CDS
AATTCAAATCATGTTGTCAT+TGG 0.330631 8_4:-31543441 Msa1323290:CDS
ATGTTCAAACTTTGAGTTAT+GGG 0.393724 8_4:-31543593 Msa1323290:CDS
AGTGGCTAGGATAGCCCTTT+CGG 0.395513 8_4:-31543649 Msa1323290:CDS
ATTCAAATCATGTTGTCATT+GGG 0.399832 8_4:-31543440 Msa1323290:CDS
TACAAAGGAGCTCGTCCTAT+TGG 0.408643 8_4:-31543672 Msa1323290:CDS
AACTTTGAGTTATGGGTTGA+GGG 0.424777 8_4:-31543586 Msa1323290:CDS
GATGTTCAAACTTTGAGTTA+TGG 0.428751 8_4:-31543594 Msa1323290:CDS
TATTGAGGATGTGGGGTTGA+AGG 0.431275 8_4:-31543475 Msa1323290:CDS
AAACTTTGAGTTATGGGTTG+AGG 0.441165 8_4:-31543587 Msa1323290:CDS
GCTATCCTAGCCACTCCAAT+AGG 0.445086 8_4:+31543657 None:intergenic
AGAACAAGGTATTGAGGATG+TGG 0.474654 8_4:-31543484 Msa1323290:CDS
TGCAAAATCAGTGACGTTTG+AGG 0.496537 8_4:-31543709 Msa1323290:CDS
TGAGGATGTGGGGTTGAAGG+TGG 0.512663 8_4:-31543472 Msa1323290:CDS
AAGTGTTGATCGAAATTCAA+AGG 0.521135 8_4:-31543853 Msa1323290:CDS
GAACAAGGTATTGAGGATGT+GGG 0.522715 8_4:-31543483 Msa1323290:CDS
GGTTGAGGGATTGGGATGGT+AGG 0.532351 8_4:-31543572 Msa1323290:CDS
AGTGTTGATCGAAATTCAAA+GGG 0.547290 8_4:-31543852 Msa1323290:CDS
GTCACCAAGAAAATCCGAAA+GGG 0.572767 8_4:+31543635 None:intergenic
GTGATTTCTAATGTGCGTGC+TGG 0.574354 8_4:+31543897 None:intergenic
GTGAGAAGGGTGACACAAGA+AGG 0.595191 8_4:-31543525 Msa1323290:CDS
TATGGGTTGAGGGATTGGGA+TGG 0.614237 8_4:-31543576 Msa1323290:CDS
AAATATCTCATAGAAATGGA+AGG 0.619843 8_4:-31543927 None:intergenic
AACAAGGTATTGAGGATGTG+GGG 0.625303 8_4:-31543482 Msa1323290:CDS
AGGAGCTCGTCCTATTGGAG+TGG 0.651439 8_4:-31543667 Msa1323290:CDS
GTACAATGCAAGAAGTACAA+AGG 0.653282 8_4:-31543687 Msa1323290:CDS
CGTCACCAAGAAAATCCGAA+AGG 0.654815 8_4:+31543634 None:intergenic
CTCGTCCTATTGGAGTGGCT+AGG 0.670537 8_4:-31543662 Msa1323290:CDS
AAAATGGTGAAGGAAAACGA+GGG 0.775535 8_4:-31543777 Msa1323290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAATTTTAGTGTGAGA+AGG - chr8_4:31543747-31543766 Msa1323290:CDS 20.0%
!!! TATTAATTTTAGTGTGAGAA+GGG - chr8_4:31543748-31543767 Msa1323290:CDS 20.0%
! TTAGAAATCACCATTATTTC+CGG - chr8_4:31543401-31543420 Msa1323290:CDS 25.0%
!! ATGTTCAAACTTTGAGTTAT+GGG - chr8_4:31543693-31543712 Msa1323290:CDS 25.0%
! AATTCAAATCATGTTGTCAT+TGG - chr8_4:31543845-31543864 Msa1323290:CDS 25.0%
! ATTCAAATCATGTTGTCATT+GGG - chr8_4:31543846-31543865 Msa1323290:CDS 25.0%
!!! TTTGGGTTTTTTGTTTCTTT+TGG - chr8_4:31543877-31543896 Msa1323290:CDS 25.0%
!!! TTGGGTTTTTTGTTTCTTTT+GGG - chr8_4:31543878-31543897 Msa1323290:CDS 25.0%
! TATGTTTTGTCCGGAAATAA+TGG + chr8_4:31543414-31543433 None:intergenic 30.0%
! ATCAACACTTATGTTTTGTC+CGG + chr8_4:31543423-31543442 None:intergenic 30.0%
!! AAGTGTTGATCGAAATTCAA+AGG - chr8_4:31543433-31543452 Msa1323290:CDS 30.0%
!! AGTGTTGATCGAAATTCAAA+GGG - chr8_4:31543434-31543453 Msa1323290:CDS 30.0%
! GATGTTCAAACTTTGAGTTA+TGG - chr8_4:31543692-31543711 Msa1323290:CDS 30.0%
TTGTGTGTAGAAAAAGAACA+AGG - chr8_4:31543788-31543807 Msa1323290:CDS 30.0%
!! AGAAAAAGAACAAGGTATTG+AGG - chr8_4:31543796-31543815 Msa1323290:CDS 30.0%
AGAAACAAAAAACCCAAAAC+TGG + chr8_4:31543875-31543894 None:intergenic 30.0%
AAAAATGGTGAAGGAAAACG+AGG - chr8_4:31543508-31543527 Msa1323290:CDS 35.0%
AAAATGGTGAAGGAAAACGA+GGG - chr8_4:31543509-31543528 Msa1323290:CDS 35.0%
AAAACGAGGGTTTACTTTCA+TGG - chr8_4:31543522-31543541 Msa1323290:CDS 35.0%
! GTACAATGCAAGAAGTACAA+AGG - chr8_4:31543599-31543618 Msa1323290:CDS 35.0%
! AAACTTTGAGTTATGGGTTG+AGG - chr8_4:31543699-31543718 Msa1323290:CDS 35.0%
! AACTTTGAGTTATGGGTTGA+GGG - chr8_4:31543700-31543719 Msa1323290:CDS 35.0%
CAACTCTTGCACGACAAAAA+TGG - chr8_4:31543493-31543512 Msa1323290:CDS 40.0%
TTGCACGACAAAAATGGTGA+AGG - chr8_4:31543499-31543518 Msa1323290:CDS 40.0%
!!! AACGTCACTGATTTTGCATG+TGG + chr8_4:31543575-31543594 None:intergenic 40.0%
TGCAAAATCAGTGACGTTTG+AGG - chr8_4:31543577-31543596 Msa1323290:CDS 40.0%
! ATAGCCCTTTCGGATTTTCT+TGG - chr8_4:31543647-31543666 Msa1323290:CDS 40.0%
GTCACCAAGAAAATCCGAAA+GGG + chr8_4:31543654-31543673 None:intergenic 40.0%
GATTGGGATGGTAGGAAAAA+TGG - chr8_4:31543722-31543741 Msa1323290:CDS 40.0%
!! AGAACAAGGTATTGAGGATG+TGG - chr8_4:31543802-31543821 Msa1323290:CDS 40.0%
!! GAACAAGGTATTGAGGATGT+GGG - chr8_4:31543803-31543822 Msa1323290:CDS 40.0%
!! AACAAGGTATTGAGGATGTG+GGG - chr8_4:31543804-31543823 Msa1323290:CDS 40.0%
! TGTCATTGGGATTCCAGTTT+TGG - chr8_4:31543859-31543878 Msa1323290:CDS 40.0%
! GTCATTGGGATTCCAGTTTT+GGG - chr8_4:31543860-31543879 Msa1323290:CDS 40.0%
GTGATTTCTAATGTGCGTGC+TGG + chr8_4:31543392-31543411 None:intergenic 45.0%
TACAAAGGAGCTCGTCCTAT+TGG - chr8_4:31543614-31543633 Msa1323290:CDS 45.0%
CGTCACCAAGAAAATCCGAA+AGG + chr8_4:31543655-31543674 None:intergenic 45.0%
! TGAGTTATGGGTTGAGGGAT+TGG - chr8_4:31543705-31543724 Msa1323290:CDS 45.0%
! GAGTTATGGGTTGAGGGATT+GGG - chr8_4:31543706-31543725 Msa1323290:CDS 45.0%
TATTGAGGATGTGGGGTTGA+AGG - chr8_4:31543811-31543830 Msa1323290:CDS 45.0%
GCTATCCTAGCCACTCCAAT+AGG + chr8_4:31543632-31543651 None:intergenic 50.0%
AGTGGCTAGGATAGCCCTTT+CGG - chr8_4:31543637-31543656 Msa1323290:CDS 50.0%
! TATGGGTTGAGGGATTGGGA+TGG - chr8_4:31543710-31543729 Msa1323290:CDS 50.0%
GTGAGAAGGGTGACACAAGA+AGG - chr8_4:31543761-31543780 Msa1323290:CDS 50.0%
AGGAGCTCGTCCTATTGGAG+TGG - chr8_4:31543619-31543638 Msa1323290:CDS 55.0%
CTCGTCCTATTGGAGTGGCT+AGG - chr8_4:31543624-31543643 Msa1323290:CDS 55.0%
GGTTGAGGGATTGGGATGGT+AGG - chr8_4:31543714-31543733 Msa1323290:CDS 55.0%
TGAGGATGTGGGGTTGAAGG+TGG - chr8_4:31543814-31543833 Msa1323290:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 31543374 31543934 31543374 ID=Msa1323290;Name=Msa1323290
chr8_4 mRNA 31543374 31543934 31543374 ID=Msa1323290-mRNA-1;Parent=Msa1323290;Name=Msa1323290-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|186
chr8_4 exon 31543374 31543934 31543374 ID=Msa1323290-mRNA-1:exon:10272;Parent=Msa1323290-mRNA-1
chr8_4 CDS 31543374 31543934 31543374 ID=Msa1323290-mRNA-1:cds;Parent=Msa1323290-mRNA-1
Gene Sequence

>Msa1323290

ATGGAAGGAAAACAACCAGCACGCACATTAGAAATCACCATTATTTCCGGACAAAACATAAGTGTTGATCGAAATTCAAAGGGAGATGATATTTATGTTGTTGTTCGTGCAGAGTCTCTCAACTCTTGCACGACAAAAATGGTGAAGGAAAACGAGGGTTTACTTTCATGGAACGAAAAATTCTTATTAGATATTTCACCACATGCAAAATCAGTGACGTTTGAGGTACAATGCAAGAAGTACAAAGGAGCTCGTCCTATTGGAGTGGCTAGGATAGCCCTTTCGGATTTTCTTGGTGACGATTTGTCGTTGCAAAGTGATGTTCAAACTTTGAGTTATGGGTTGAGGGATTGGGATGGTAGGAAAAATGGTGTTATTAATTTTAGTGTGAGAAGGGTGACACAAGAAGGTAATTTGTGTGTAGAAAAAGAACAAGGTATTGAGGATGTGGGGTTGAAGGTGGATGAGAAGAATTCAAATCATGTTGTCATTGGGATTCCAGTTTTGGGTTTTTTGTTTCTTTTGGGCTATTCTCTTTTCTCTTTTGTTCGTCGAAAGTAA

Protein sequence

>Msa1323290

MEGKQPARTLEITIISGQNISVDRNSKGDDIYVVVRAESLNSCTTKMVKENEGLLSWNEKFLLDISPHAKSVTFEVQCKKYKGARPIGVARIALSDFLGDDLSLQSDVQTLSYGLRDWDGRKNGVINFSVRRVTQEGNLCVEKEQGIEDVGLKVDEKNSNHVVIGIPVLGFLFLLGYSLFSFVRRK*