Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323290 | XP_013444919.1 | 95.699 | 186 | 7 | 1 | 1 | 186 | 1 | 185 | 5.23e-124 | 357 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323290 | A0A072TN82 | 95.699 | 186 | 7 | 1 | 1 | 186 | 1 | 185 | 2.50e-124 | 357 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015090 | Msa1323290 | 0.802551 | 5.316209e-49 | -8.615850e-47 |
| Msa0017320 | Msa1323290 | 0.805798 | 1.121958e-49 | -8.615850e-47 |
| Msa0037880 | Msa1323290 | 0.820231 | 7.652196e-53 | -8.615850e-47 |
| Msa0042550 | Msa1323290 | 0.808609 | 2.847725e-50 | -8.615850e-47 |
| Msa0043980 | Msa1323290 | 0.811737 | 6.026951e-51 | -8.615850e-47 |
| Msa0049750 | Msa1323290 | 0.806606 | 7.581403e-50 | -8.615850e-47 |
| Msa0058310 | Msa1323290 | 0.804452 | 2.145377e-49 | -8.615850e-47 |
| Msa0100090 | Msa1323290 | 0.804582 | 2.015845e-49 | -8.615850e-47 |
| Msa0109590 | Msa1323290 | 0.808517 | 2.979003e-50 | -8.615850e-47 |
| Msa0124550 | Msa1323290 | 0.828658 | 7.956729e-55 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323290 | MtrunA17_Chr8g0350281 | 95.699 | 186 | 7 | 1 | 1 | 186 | 1 | 185 | 4.81e-128 | 357 |
| Msa1323290 | MtrunA17_Chr8g0350221 | 53.933 | 178 | 58 | 6 | 7 | 167 | 5 | 175 | 2.70e-52 | 165 |
| Msa1323290 | MtrunA17_Chr8g0350241 | 53.672 | 177 | 61 | 5 | 7 | 168 | 5 | 175 | 5.89e-51 | 162 |
| Msa1323290 | MtrunA17_Chr8g0350231 | 51.099 | 182 | 58 | 5 | 7 | 167 | 5 | 176 | 2.08e-49 | 158 |
| Msa1323290 | MtrunA17_Chr8g0350271 | 51.412 | 177 | 64 | 5 | 7 | 167 | 5 | 175 | 5.05e-49 | 157 |
| Msa1323290 | MtrunA17_Chr3g0109941 | 51.412 | 177 | 65 | 5 | 7 | 168 | 5 | 175 | 9.01e-49 | 156 |
| Msa1323290 | MtrunA17_Chr8g0350291 | 51.705 | 176 | 64 | 5 | 7 | 167 | 5 | 174 | 7.28e-48 | 154 |
| Msa1323290 | MtrunA17_Chr8g0350211 | 37.500 | 128 | 76 | 3 | 7 | 131 | 2 | 128 | 4.73e-20 | 82.8 |
| Msa1323290 | MtrunA17_Chr8g0350191 | 36.923 | 130 | 74 | 5 | 7 | 131 | 2 | 128 | 3.14e-19 | 80.5 |
| Msa1323290 | MtrunA17_Chr8g0350201 | 36.923 | 130 | 74 | 5 | 7 | 131 | 2 | 128 | 4.41e-19 | 80.1 |
Find 33 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGAGGGTTTACTTTCA+TGG | 0.320231 | 8_4:-31543764 | Msa1323290:CDS |
| AAAATGGTGAAGGAAAACGA+GGG | 0.775535 | 8_4:-31543777 | Msa1323290:CDS |
| AAACTTTGAGTTATGGGTTG+AGG | 0.441165 | 8_4:-31543587 | Msa1323290:CDS |
| AAATATCTCATAGAAATGGA+AGG | 0.619843 | 8_4:-31543927 | None:intergenic |
| AACAAGGTATTGAGGATGTG+GGG | 0.625303 | 8_4:-31543482 | Msa1323290:CDS |
| AACTTTGAGTTATGGGTTGA+GGG | 0.424777 | 8_4:-31543586 | Msa1323290:CDS |
| AAGTGTTGATCGAAATTCAA+AGG | 0.521135 | 8_4:-31543853 | Msa1323290:CDS |
| AATTCAAATCATGTTGTCAT+TGG | 0.330631 | 8_4:-31543441 | Msa1323290:CDS |
| AGAACAAGGTATTGAGGATG+TGG | 0.474654 | 8_4:-31543484 | Msa1323290:CDS |
| AGGAGCTCGTCCTATTGGAG+TGG | 0.651439 | 8_4:-31543667 | Msa1323290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGTGTGTAGAAAAAGAACA+AGG | - | chr8_4:31543788-31543807 | Msa1323290:CDS | 30.0% | |
| AGAAACAAAAAACCCAAAAC+TGG | + | chr8_4:31543875-31543894 | None:intergenic | 30.0% | |
| AAAAATGGTGAAGGAAAACG+AGG | - | chr8_4:31543508-31543527 | Msa1323290:CDS | 35.0% | |
| AAAATGGTGAAGGAAAACGA+GGG | - | chr8_4:31543509-31543528 | Msa1323290:CDS | 35.0% | |
| AAAACGAGGGTTTACTTTCA+TGG | - | chr8_4:31543522-31543541 | Msa1323290:CDS | 35.0% | |
| CAACTCTTGCACGACAAAAA+TGG | - | chr8_4:31543493-31543512 | Msa1323290:CDS | 40.0% | |
| TTGCACGACAAAAATGGTGA+AGG | - | chr8_4:31543499-31543518 | Msa1323290:CDS | 40.0% | |
| TGCAAAATCAGTGACGTTTG+AGG | - | chr8_4:31543577-31543596 | Msa1323290:CDS | 40.0% | |
| GTCACCAAGAAAATCCGAAA+GGG | + | chr8_4:31543654-31543673 | None:intergenic | 40.0% | |
| GATTGGGATGGTAGGAAAAA+TGG | - | chr8_4:31543722-31543741 | Msa1323290:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 31543374 | 31543934 | 31543374 | ID=Msa1323290;Name=Msa1323290 |
| chr8_4 | mRNA | 31543374 | 31543934 | 31543374 | ID=Msa1323290-mRNA-1;Parent=Msa1323290;Name=Msa1323290-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|186 |
| chr8_4 | exon | 31543374 | 31543934 | 31543374 | ID=Msa1323290-mRNA-1:exon:10272;Parent=Msa1323290-mRNA-1 |
| chr8_4 | CDS | 31543374 | 31543934 | 31543374 | ID=Msa1323290-mRNA-1:cds;Parent=Msa1323290-mRNA-1 |
| Gene Sequence |
>Msa1323290 ATGGAAGGAAAACAACCAGCACGCACATTAGAAATCACCATTATTTCCGGACAAAACATAAGTGTTGATCGAAATTCAAAGGGAGATGATATTTATGTTGTTGTTCGTGCAGAGTCTCTCAACTCTTGCACGACAAAAATGGTGAAGGAAAACGAGGGTTTACTTTCATGGAACGAAAAATTCTTATTAGATATTTCACCACATGCAAAATCAGTGACGTTTGAGGTACAATGCAAGAAGTACAAAGGAGCTCGTCCTATTGGAGTGGCTAGGATAGCCCTTTCGGATTTTCTTGGTGACGATTTGTCGTTGCAAAGTGATGTTCAAACTTTGAGTTATGGGTTGAGGGATTGGGATGGTAGGAAAAATGGTGTTATTAATTTTAGTGTGAGAAGGGTGACACAAGAAGGTAATTTGTGTGTAGAAAAAGAACAAGGTATTGAGGATGTGGGGTTGAAGGTGGATGAGAAGAATTCAAATCATGTTGTCATTGGGATTCCAGTTTTGGGTTTTTTGTTTCTTTTGGGCTATTCTCTTTTCTCTTTTGTTCGTCGAAAGTAA |
| Protein sequence |
>Msa1323290 MEGKQPARTLEITIISGQNISVDRNSKGDDIYVVVRAESLNSCTTKMVKENEGLLSWNEKFLLDISPHAKSVTFEVQCKKYKGARPIGVARIALSDFLGDDLSLQSDVQTLSYGLRDWDGRKNGVINFSVRRVTQEGNLCVEKEQGIEDVGLKVDEKNSNHVVIGIPVLGFLFLLGYSLFSFVRRK* |
