AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099650 MtrunA17_Chr1g0150791 89.859 1065 59 1 1 1016 1 1065 0.0 1935
Msa0099650 MtrunA17_Chr2g0300211 34.445 1199 589 29 2 1015 1 1187 4.37e-171 532
Msa0099650 MtrunA17_Chr4g0070741 35.734 1097 565 24 24 1015 9 1070 1.36e-169 525
Msa0099650 MtrunA17_Chr6g0483131 38.835 206 121 3 44 246 78 281 4.38e-34 141
Msa0099650 MtrunA17_Chr6g0483131 27.208 419 215 13 618 1012 514 866 6.99e-27 118
Msa0099650 MtrunA17_Chr8g0392621 36.782 174 104 3 76 246 126 296 8.31e-27 118
Msa0099650 MtrunA17_Chr8g0392621 25.761 427 220 15 618 1016 535 892 9.65e-25 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099650 AT5G26160.3 37.202 1008 527 25 18 994 8 940 1.86e-169 522
Msa0099650 AT5G26160.2 37.202 1008 527 25 18 994 8 940 1.90e-169 522
Msa0099650 AT5G26160.1 37.202 1008 527 25 18 994 8 940 1.90e-169 522
Msa0099650 AT5G20610.1 35.591 635 313 16 464 1016 544 1164 3.90e-92 319
Msa0099650 AT5G20610.1 47.034 236 109 4 26 260 13 233 1.05e-57 217
Msa0099650 AT1G42550.1 37.725 167 98 4 85 246 123 288 5.45e-26 115
Msa0099650 AT1G42550.1 25.478 314 156 12 552 826 458 732 3.74e-12 71.2

Find 220 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGATCTTCAAGAATTT+TGG 0.094286 1_3:+5849212 None:intergenic
ATGAAGTGAATGAATTTATT+AGG 0.170610 1_3:-5850972 Msa0099650:five_prime_UTR
TCTGATTTATCTTTAATTAT+TGG 0.172054 1_3:-5849291 Msa0099650:CDS
TGGCAGAAAGCGCAGTTATT+TGG 0.193805 1_3:-5848225 Msa0099650:CDS
CATGGATAGATTTCCTAAAT+TGG 0.196193 1_3:+5847692 None:intergenic
GTACATAGATGTTCGGTTTA+TGG 0.206716 1_3:-5850461 Msa0099650:CDS
GAGATGGTAGAAGGCTTAAA+AGG 0.248939 1_3:+5850791 None:intergenic
AATCTGTCTTCAAGTTAATA+AGG 0.259711 1_3:+5850147 None:intergenic
CATCCAAGGAGAACATAATT+TGG 0.267826 1_3:-5848342 Msa0099650:CDS
CACTTAAGTAGTGTTCTTCT+TGG 0.277300 1_3:+5849586 None:intergenic
ACTGAAACTGATTCAAATAT+TGG 0.283455 1_3:-5850875 Msa0099650:CDS
CACTTTGGGAACATGATATC+AGG 0.285981 1_3:+5847750 None:intergenic
ATCCGACACAATATGCTTAA+TGG 0.290782 1_3:+5848813 None:intergenic
TGGTGGTAACCGACAAGTTT+GGG 0.293482 1_3:-5847976 Msa0099650:CDS
AGAACCTGGTCTGGTGGATT+TGG 0.298861 1_3:-5849132 Msa0099650:CDS
TTCACCAATCGAAGGTAATA+TGG 0.301986 1_3:+5849077 None:intergenic
TGATTTATGCTTCTGTTGTT+GGG 0.305019 1_3:-5850388 Msa0099650:CDS
TCTTCATCGGATATTTCAAT+TGG 0.308153 1_3:+5849105 None:intergenic
TTGTGCGAGCCTCATCATTC+AGG 0.308272 1_3:-5848974 Msa0099650:CDS
AGGTTGGTTGGAAAAGCTTT+AGG 0.311083 1_3:-5850254 Msa0099650:CDS
TTGATTTATGCTTCTGTTGT+TGG 0.316516 1_3:-5850389 Msa0099650:CDS
CACTGAGTTTAGAAGAGTTA+TGG 0.323931 1_3:-5850313 Msa0099650:CDS
TAATGTGGATGAGATTATTA+AGG 0.327179 1_3:-5849784 Msa0099650:CDS
ACTGGTTCATAATTTCTTAG+AGG 0.327756 1_3:+5848161 None:intergenic
ACTTACCAAAGAAGAGAAAA+AGG 0.328492 1_3:+5851212 None:intergenic
CTTTGAGTTTCAATGGTGTT+AGG 0.329725 1_3:-5850574 Msa0099650:CDS
GGCAGAAAGCGCAGTTATTT+GGG 0.332530 1_3:-5848224 Msa0099650:CDS
ACAAGAAATTGTCTTCAAAA+TGG 0.335837 1_3:-5850691 Msa0099650:CDS
CACTGGATACCGTATGTTCT+AGG 0.342638 1_3:-5849722 Msa0099650:CDS
AAGACTAACAAGGAAGCTTC+TGG 0.343748 1_3:-5848787 Msa0099650:CDS
AATTGTCTTCAAAATGGAAT+TGG 0.344941 1_3:-5850685 Msa0099650:CDS
TGTTTCCCAATGTCATGTTC+AGG 0.348192 1_3:+5850362 None:intergenic
GTAAGTGAGGATCTGTTGAT+AGG 0.349589 1_3:-5850716 Msa0099650:CDS
TTATGGGGTGATAAAAGTTC+AGG 0.358120 1_3:-5850296 Msa0099650:CDS
AAGGATGATTTAATGAGATT+TGG 0.360201 1_3:-5850194 Msa0099650:CDS
GCTCTGACATTGTTCACTTT+GGG 0.360351 1_3:+5847736 None:intergenic
TGCTCTGACATTGTTCACTT+TGG 0.361069 1_3:+5847735 None:intergenic
ACCAGTTGGAGTTCCAATAC+TGG 0.365800 1_3:-5848144 Msa0099650:CDS
AATCCACCAGACCAGGTTCT+TGG 0.366360 1_3:+5849135 None:intergenic
TGAAGTGAATGAATTTATTA+GGG 0.366744 1_3:-5850971 Msa0099650:five_prime_UTR
TGAGATTTGGTGCTAGTAAT+GGG 0.369936 1_3:-5850181 Msa0099650:CDS
CGACGATCTTGATGGTTTGA+TGG 0.371384 1_3:-5848411 Msa0099650:CDS
AATGAAGCAGTGATGAATTC+TGG 0.373320 1_3:-5850035 Msa0099650:CDS
AAAGGAATATTGTGTGGAAA+TGG 0.373622 1_3:+5851230 None:intergenic
CTTCATGTTCATTCGATTGA+GGG 0.378234 1_3:-5850602 Msa0099650:CDS
AATATTTGTTAAAACTTTCA+AGG 0.378277 1_3:+5850655 None:intergenic
AACTGAATCCTCAATCTTCA+TGG 0.382045 1_3:+5849632 None:intergenic
AATGCGAATGAGGAGGGATT+GGG 0.384507 1_3:-5849357 Msa0099650:CDS
ACTGAGTTTAGAAGAGTTAT+GGG 0.386969 1_3:-5850312 Msa0099650:CDS
GAAAATGAGGCTCTAGCTTC+AGG 0.387737 1_3:-5849399 Msa0099650:CDS
AAGAGTTGACATACATGAAA+TGG 0.388521 1_3:-5848105 Msa0099650:CDS
CAATGGTTGAGACTTGATTC+TGG 0.388547 1_3:-5848368 Msa0099650:CDS
CCTTCGATTGGTGAAGGGCT+TGG 0.388984 1_3:-5849069 Msa0099650:CDS
AACATCCCCGACAATGTTGC+TGG 0.391116 1_3:-5850122 Msa0099650:CDS
ATGGTGGTAACCGACAAGTT+TGG 0.394268 1_3:-5847977 Msa0099650:CDS
ATCAAGGAGATTCATATTGC+AGG 0.400807 1_3:-5848014 Msa0099650:CDS
GTGATAAAAGTTCAGGGAAA+TGG 0.403771 1_3:-5850289 Msa0099650:CDS
GCAGTGATGAATTCTGGTTC+AGG 0.403914 1_3:-5850029 Msa0099650:CDS
TCAATCTTGTTAATAGTCAT+AGG 0.404954 1_3:+5848515 None:intergenic
TATAATGATGGGTTGGGAAA+TGG 0.405336 1_3:-5848269 Msa0099650:CDS
ACCAGTATTGGAACTCCAAC+TGG 0.408338 1_3:+5848143 None:intergenic
TATGGTGGTCGCGTCGTGTC+TGG 0.409194 1_3:-5850443 Msa0099650:CDS
AAGAAAAGATCATGTCCAAT+AGG 0.410891 1_3:+5847600 None:intergenic
GCTAGATTAAATGTTAGTTT+CGG 0.410989 1_3:-5850230 Msa0099650:CDS
GAACCGTCCAATACCCTTGA+TGG 0.412288 1_3:+5850506 None:intergenic
GGACTTCCTTTGAGTTTCAA+TGG 0.413613 1_3:-5850581 Msa0099650:CDS
TTTAAGACAGTTTGAAAATG+AGG 0.413809 1_3:-5849412 Msa0099650:CDS
TGATAAATTAATGCAACAAT+GGG 0.414227 1_3:-5849187 Msa0099650:CDS
ATCTTGATTCAACAATTTGT+TGG 0.416147 1_3:+5847833 None:intergenic
ATCACAGAATTATATAATGA+TGG 0.420774 1_3:-5848281 Msa0099650:CDS
AAGGAAAGGAAAAGGGTGAT+TGG 0.421702 1_3:+5851179 None:intergenic
ATTAAGCAAAGCTCTTTACT+TGG 0.426857 1_3:-5850822 Msa0099650:CDS
TAATGCGAATGAGGAGGGAT+TGG 0.434699 1_3:-5849358 Msa0099650:CDS
AACTTTGGTACATAGATGTT+CGG 0.436998 1_3:-5850468 Msa0099650:CDS
GGTTAAGTGTGCATTGGAAA+AGG 0.437103 1_3:-5850553 Msa0099650:CDS
CTAAAACCAGCAACATTGTC+GGG 0.437682 1_3:+5850116 None:intergenic
GTTCCAAATTATGTTCTCCT+TGG 0.441194 1_3:+5848339 None:intergenic
AGGAAAAGGGTGATTGGAAA+TGG 0.443271 1_3:+5851185 None:intergenic
TTCTTAGAGGATCTCTGTGC+TGG 0.444411 1_3:+5848174 None:intergenic
CTTCATCGGATATTTCAATT+GGG 0.444598 1_3:+5849106 None:intergenic
GGAGTACAAATCTACAGAAT+CGG 0.444771 1_3:+5847561 None:intergenic
ATGAGATTTGGTGCTAGTAA+TGG 0.445911 1_3:-5850182 Msa0099650:CDS
ACTGCGTCTCAGCAACAATC+CGG 0.449045 1_3:-5847951 Msa0099650:CDS
TTCTACCCCTTGTTCACTGA+TGG 0.449718 1_3:+5849881 None:intergenic
TCGTTCGAATTTGATCAAAC+AGG 0.452174 1_3:-5849843 Msa0099650:CDS
GACTTGCCCATCAAGGGTAT+TGG 0.456422 1_3:-5850513 Msa0099650:CDS
GAAATCAACGACGATCTTGA+TGG 0.458924 1_3:-5848419 Msa0099650:CDS
ACTGATAAGGTGAAAATAGA+TGG 0.459395 1_3:-5848245 Msa0099650:CDS
TAGAGACTAATGCACACATT+AGG 0.459516 1_3:-5847776 Msa0099650:CDS
TAGGAATGTGAATGTGGATA+TGG 0.460785 1_3:-5849703 Msa0099650:CDS
TCTGTTTCAGGAAGGGATCA+TGG 0.464848 1_3:-5848625 Msa0099650:intron
TCTTCATGTTCATTCGATTG+AGG 0.468038 1_3:-5850603 Msa0099650:CDS
AATTCTCCAAGAACCTGGTC+TGG 0.470655 1_3:-5849141 Msa0099650:CDS
GAATTATATAATGATGGGTT+GGG 0.472771 1_3:-5848275 Msa0099650:CDS
TTTCTTAAAGACGCTGGCAT+TGG 0.474864 1_3:-5849493 Msa0099650:CDS
AGATCCTCACTTACAAATCT+CGG 0.474977 1_3:+5850725 None:intergenic
TTTCGGTTATCAAGTAATGA+AGG 0.475758 1_3:-5850213 Msa0099650:CDS
AAGCAACAAAGGAAAGGAAA+AGG 0.477857 1_3:+5851171 None:intergenic
AGAATTATATAATGATGGGT+TGG 0.478189 1_3:-5848276 Msa0099650:CDS
TGCGGAGTTTGATGAAACTT+TGG 0.478584 1_3:-5850483 Msa0099650:CDS
GAGTACAAATCTACAGAATC+GGG 0.479741 1_3:+5847562 None:intergenic
TGTCAGTAACGTTGGATCAA+TGG 0.480802 1_3:-5848385 Msa0099650:CDS
GCCTGAGCTAAAACCAGTAT+TGG 0.481373 1_3:+5848131 None:intergenic
TAAGTGAGGATCTGTTGATA+GGG 0.484135 1_3:-5850715 Msa0099650:CDS
TTGAAAATTCTCCAAGAACC+TGG 0.492352 1_3:-5849146 Msa0099650:CDS
AATTATTGGTGCCGCGGAAG+AGG 0.495603 1_3:-5849277 Msa0099650:CDS
GACAATATTACACATGCCAC+TGG 0.497586 1_3:+5847911 None:intergenic
CTGATAAATTAATGCAACAA+TGG 0.498084 1_3:-5849188 Msa0099650:CDS
GATTTATGCTTCTGTTGTTG+GGG 0.498131 1_3:-5850387 Msa0099650:CDS
GATCCTCACTTACAAATCTC+GGG 0.499409 1_3:+5850726 None:intergenic
AACAGATAAAGACAAATGCT+TGG 0.500593 1_3:-5848564 Msa0099650:CDS
AGCATAAATCAAGAATCGCT+TGG 0.502217 1_3:+5850400 None:intergenic
AGATGGTAGAAGGCTTAAAA+GGG 0.502789 1_3:+5850792 None:intergenic
TCAATCTAGATTCAAAATCA+AGG 0.504472 1_3:-5848030 Msa0099650:CDS
ACGCAGTTCCCCAAACTTGT+CGG 0.504742 1_3:+5847967 None:intergenic
GGGAACCTGAACATGACATT+GGG 0.508776 1_3:-5850367 Msa0099650:CDS
AACAATGGGGTTTAGATGAG+AGG 0.511115 1_3:-5849173 Msa0099650:CDS
GTCAAGAAACTCTGACGTCT+CGG 0.513109 1_3:+5849608 None:intergenic
GTTGGTGAGATTTGAGAAGG+TGG 0.514764 1_3:+5851271 None:intergenic
TAATTCAGCACGGGCAGACT+CGG 0.515926 1_3:-5849952 Msa0099650:CDS
GCTTCCAAATCCACCAGACC+AGG 0.517383 1_3:+5849128 None:intergenic
ATCAATCATTAATGCAGAGA+AGG 0.520043 1_3:-5849239 Msa0099650:CDS
ATATTTGTTAAAACTTTCAA+GGG 0.523046 1_3:+5850656 None:intergenic
ATGCCTTTGCTAGATATCAC+TGG 0.527711 1_3:-5848841 Msa0099650:CDS
GTTGGGAAATGGCACTGATA+AGG 0.529156 1_3:-5848258 Msa0099650:CDS
AGCAACAATCCGGGTTGCGT+TGG 0.533759 1_3:-5847941 Msa0099650:CDS
AGCAACAAAGGAAAGGAAAA+GGG 0.534880 1_3:+5851172 None:intergenic
TTATCTGTTAAATAACTACA+TGG 0.535658 1_3:+5848578 None:intergenic
ATAGGGATGTCGTAAAGCTT+CGG 0.535896 1_3:-5850085 Msa0099650:CDS
TCACAGAATTATATAATGAT+GGG 0.538575 1_3:-5848280 Msa0099650:CDS
ATATTACCTTCGATTGGTGA+AGG 0.538823 1_3:-5849075 Msa0099650:CDS
ACATGACATTGGGAAACATC+AGG 0.539471 1_3:-5850357 Msa0099650:CDS
TAACGCAAGATGTGATTCAC+TGG 0.542746 1_3:-5849739 Msa0099650:CDS
CTAAGAAATTATGAACCAGT+TGG 0.543091 1_3:-5848158 Msa0099650:CDS
TGTCTTAAAAGCTTCTCTCT+AGG 0.544252 1_3:+5849426 None:intergenic
TCTACCATCCATGAAGATTG+AGG 0.546459 1_3:-5849640 Msa0099650:CDS
TGTTGAATCAAGATATCTTG+TGG 0.547330 1_3:-5847824 Msa0099650:CDS
GATGGATCTGTCAGTAACGT+TGG 0.547375 1_3:-5848393 Msa0099650:CDS
TCATTTCGCTCAAGCTGTGA+CGG 0.549382 1_3:-5849462 Msa0099650:CDS
TCTCCAAGAACCTGGTCTGG+TGG 0.549400 1_3:-5849138 Msa0099650:CDS
TACACTTGAACATAACTATG+AGG 0.551566 1_3:-5849328 Msa0099650:CDS
AAGCTTCTCTCTAGGAGACA+TGG 0.552937 1_3:+5849434 None:intergenic
ATTCTTAGCATTTCTGAAGA+GGG 0.553950 1_3:+5848996 None:intergenic
GTCTAGCATGTCAAATGAAG+AGG 0.553980 1_3:-5848462 Msa0099650:CDS
TTGCGTTGGTTGCTTTCCAG+TGG 0.555258 1_3:-5847927 Msa0099650:CDS
CCTATATCAAAAGCTTGATG+AGG 0.555755 1_3:-5849985 Msa0099650:CDS
GTAGTTGGTGAGATTTGAGA+AGG 0.556167 1_3:+5851268 None:intergenic
CTATATCAAAAGCTTGATGA+GGG 0.556939 1_3:-5849984 Msa0099650:CDS
CTTGATTCTGGCATCATCCA+AGG 0.557881 1_3:-5848356 Msa0099650:CDS
AATTCTTAGCATTTCTGAAG+AGG 0.558323 1_3:+5848995 None:intergenic
ACTAAAACCAGCAACATTGT+CGG 0.558844 1_3:+5850115 None:intergenic
TTAGCATTTCTGAAGAGGGA+AGG 0.560033 1_3:+5849000 None:intergenic
TAATATGGACGGTTCTTCAT+CGG 0.561202 1_3:+5849092 None:intergenic
CCGCGGAAGAGGAGTATGAA+AGG 0.563504 1_3:-5849266 Msa0099650:CDS
CGCGGAAGAGGAGTATGAAA+GGG 0.564447 1_3:-5849265 Msa0099650:CDS
ATTTGAAAGCAACAAAGGAA+AGG 0.565310 1_3:+5851165 None:intergenic
CACATTAATGATTTCAACTG+TGG 0.565922 1_3:+5849800 None:intergenic
CTGCGTCTCAGCAACAATCC+GGG 0.567017 1_3:-5847950 Msa0099650:CDS
ATCTTTAATTATTGGTGCCG+CGG 0.568539 1_3:-5849283 Msa0099650:CDS
AGCTTCTCTCTAGGAGACAT+GGG 0.568860 1_3:+5849435 None:intergenic
CTAACACCATTGAAACTCAA+AGG 0.571841 1_3:+5850575 None:intergenic
TTTGCAGACTTGCCCATCAA+GGG 0.571889 1_3:-5850519 Msa0099650:CDS
TATTACCTTCGATTGGTGAA+GGG 0.575420 1_3:-5849074 Msa0099650:CDS
ATTCACATTCCTAGAACATA+CGG 0.576528 1_3:+5849713 None:intergenic
GGGGAACCTGAACATGACAT+TGG 0.579850 1_3:-5850368 Msa0099650:CDS
TATGGGGTGATAAAAGTTCA+GGG 0.580001 1_3:-5850295 Msa0099650:CDS
GTGTTAGGTTAAGTGTGCAT+TGG 0.583787 1_3:-5850559 Msa0099650:CDS
AACCGTCCAATACCCTTGAT+GGG 0.583938 1_3:+5850507 None:intergenic
ATCATTAATGCAGAGAAGGA+AGG 0.585207 1_3:-5849235 Msa0099650:CDS
CCGTCCATATTACCTTCGAT+TGG 0.585444 1_3:-5849081 Msa0099650:CDS
ATCCATTAAGCATATTGTGT+CGG 0.586015 1_3:-5848815 Msa0099650:CDS
TCTGAAGAGGGAAGGACACA+TGG 0.586443 1_3:+5849008 None:intergenic
GATTGATTTGAAAGCAACAA+AGG 0.587578 1_3:+5851160 None:intergenic
ATGCGAATGAGGAGGGATTG+GGG 0.589104 1_3:-5849356 Msa0099650:CDS
TCGTTTGTTCAAACTATGAG+CGG 0.591262 1_3:-5849045 Msa0099650:CDS
ATTGGATATACTTCTGTACA+TGG 0.591836 1_3:-5848908 Msa0099650:CDS
GGAAAGCAACCAACGCAACC+CGG 0.594488 1_3:+5847932 None:intergenic
GAATCCTCAATCTTCATGGA+TGG 0.594679 1_3:+5849636 None:intergenic
ATTTCTCCACGAATGCATGA+TGG 0.595591 1_3:+5848437 None:intergenic
AGGGGTAGAAACATCTGAAG+AGG 0.598445 1_3:-5849868 Msa0099650:CDS
CATAGATGTTCGGTTTATGG+TGG 0.601478 1_3:-5850458 Msa0099650:CDS
GAACTTCAATAATTCAGCAC+GGG 0.606552 1_3:-5849961 Msa0099650:CDS
GAGGCACCATCATGCATTCG+TGG 0.607479 1_3:-5848443 Msa0099650:CDS
ACAATGGGGTTTAGATGAGA+GGG 0.609250 1_3:-5849172 Msa0099650:CDS
ACAAATCTACAGAATCGGGA+AGG 0.611751 1_3:+5847566 None:intergenic
AAGGGTATTGGACGGTTCTG+CGG 0.613657 1_3:-5850501 Msa0099650:CDS
CACAGTTGAAATCATTAATG+TGG 0.613849 1_3:-5849799 Msa0099650:CDS
CAAATCTACAGAATCGGGAA+GGG 0.613898 1_3:+5847567 None:intergenic
GAGTCTGAAAATAAAGATGT+TGG 0.617219 1_3:-5850902 Msa0099650:CDS
TGCCCATCAAGGGTATTGGA+CGG 0.622258 1_3:-5850509 Msa0099650:CDS
GATTAGAAGCCTGAATGATG+AGG 0.627712 1_3:+5848965 None:intergenic
ATGACATCATCCAAGCTATG+TGG 0.628164 1_3:+5849534 None:intergenic
GCAGACTCGGAGTCATCTCA+AGG 0.629555 1_3:-5849939 Msa0099650:CDS
CTAACAAGGAAGCTTCTGGA+AGG 0.630482 1_3:-5848783 Msa0099650:intron
CCAAGCCCTTCACCAATCGA+AGG 0.641764 1_3:+5849069 None:intergenic
GAGTGATTTCTTAAAGACGC+TGG 0.643107 1_3:-5849499 Msa0099650:CDS
TTTCCAGTGATATCTAGCAA+AGG 0.643248 1_3:+5848838 None:intergenic
TTTGTTCAAACTATGAGCGG+AGG 0.644551 1_3:-5849042 Msa0099650:CDS
GGAACTTCAATAATTCAGCA+CGG 0.650508 1_3:-5849962 Msa0099650:CDS
CCAATCGAAGGTAATATGGA+CGG 0.651476 1_3:+5849081 None:intergenic
AAGTGAGGATCTGTTGATAG+GGG 0.655616 1_3:-5850714 Msa0099650:CDS
AACATGGTTATCATGTCATG+TGG 0.657702 1_3:+5851066 None:intergenic
CCTTTCATACTCCTCTTCCG+CGG 0.659309 1_3:+5849266 None:intergenic
AGCACCACATCATTCTGAGT+CGG 0.662136 1_3:+5850062 None:intergenic
AACATCAGGTTGATCTCACT+AGG 0.663911 1_3:-5850343 Msa0099650:CDS
TGAGATTTGAGAAGGTGGAG+TGG 0.668267 1_3:+5851276 None:intergenic
GGTGGTAACCGACAAGTTTG+GGG 0.668885 1_3:-5847975 Msa0099650:CDS
ATGTTCTAGGAATGTGAATG+TGG 0.669689 1_3:-5849709 Msa0099650:CDS
GATGGTAGAAGGCTTAAAAG+GGG 0.670150 1_3:+5850793 None:intergenic
AAACCCGAGATTTGTAAGTG+AGG 0.672556 1_3:-5850729 Msa0099650:CDS
TTTGGGAGAGCACATAACAG+TGG 0.673391 1_3:-5848207 Msa0099650:CDS
CTGAGTTTAGAAGAGTTATG+GGG 0.676392 1_3:-5850311 Msa0099650:CDS
TAAAACCAGCAACATTGTCG+GGG 0.686372 1_3:+5850117 None:intergenic
TATATCAAAAGCTTGATGAG+GGG 0.686474 1_3:-5849983 Msa0099650:CDS
TAAAAGACAGCAAAACAACA+TGG 0.692020 1_3:+5851050 None:intergenic
GATAAATTAATGCAACAATG+GGG 0.695430 1_3:-5849186 Msa0099650:CDS
TCGGCCGACTCAGAATGATG+TGG 0.705072 1_3:-5850066 Msa0099650:CDS
GTCGGATGCTAAGACTAACA+AGG 0.709457 1_3:-5848797 Msa0099650:CDS
CATAACTCTTCTAAACTCAG+TGG 0.721585 1_3:+5850314 None:intergenic
TAACTATGAGGATTATGACG+TGG 0.723957 1_3:-5849316 Msa0099650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAAGAC+AGG + chr1_3:5847766-5847785 None:intergenic 10.0%
!!! TTTTTTTTTTTATCTATCAT+AGG - chr1_3:5847776-5847795 Msa0099650:CDS 10.0%
!!! ATATTTGTTAAAACTTTCAA+GGG + chr1_3:5848214-5848233 None:intergenic 15.0%
!!! AATATTTGTTAAAACTTTCA+AGG + chr1_3:5848215-5848234 None:intergenic 15.0%
!!! TTGAAAGTTTTAACAAATAT+TGG - chr1_3:5848214-5848233 Msa0099650:CDS 15.0%
!! TCTGATTTATCTTTAATTAT+TGG - chr1_3:5849576-5849595 Msa0099650:CDS 15.0%
!! ATGAAGTGAATGAATTTATT+AGG - chr1_3:5847895-5847914 Msa0099650:CDS 20.0%
!! TGAAGTGAATGAATTTATTA+GGG - chr1_3:5847896-5847915 Msa0099650:CDS 20.0%
!! TGATAAATTAATGCAACAAT+GGG - chr1_3:5849680-5849699 Msa0099650:CDS 20.0%
!! AAAAATAGGTAACGATATAT+TGG - chr1_3:5849941-5849960 Msa0099650:CDS 20.0%
!! TTATCTGTTAAATAACTACA+TGG + chr1_3:5850292-5850311 None:intergenic 20.0%
!!! ATTGAATCTCTTTTAATAGA+AGG - chr1_3:5850370-5850389 Msa0099650:CDS 20.0%
!!! TTGAATCTCTTTTAATAGAA+GGG - chr1_3:5850371-5850390 Msa0099650:CDS 20.0%
!! ATCACAGAATTATATAATGA+TGG - chr1_3:5850586-5850605 Msa0099650:CDS 20.0%
!! TCACAGAATTATATAATGAT+GGG - chr1_3:5850587-5850606 Msa0099650:CDS 20.0%
!!! TTTACAGTTTTACTTTTACA+TGG + chr1_3:5851196-5851215 None:intergenic 20.0%
!!! TTGACATGTATTGTATTTTA+TGG - chr1_3:5851244-5851263 Msa0099650:five_prime_UTR 20.0%
! AGAAGAAGAAAAAAAGTAGT+TGG + chr1_3:5847617-5847636 None:intergenic 25.0%
!! ATATTCCTTTTTCTCTTCTT+TGG - chr1_3:5847650-5847669 Msa0099650:three_prime_UTR 25.0%
!!! TAGAATGTTTTAAAATCGAG+AGG + chr1_3:5847740-5847759 None:intergenic 25.0%
! AACAAGATCAGTTAAAAACA+TGG + chr1_3:5847854-5847873 None:intergenic 25.0%
!! TTATTTTCAGACTCAAAGTT+TGG + chr1_3:5847959-5847978 None:intergenic 25.0%
!! ACTGAAACTGATTCAAATAT+TGG - chr1_3:5847992-5848011 Msa0099650:CDS 25.0%
!!! TAAGTTGAGATTTTGACAAA+CGG + chr1_3:5848114-5848133 None:intergenic 25.0%
! ACAAGAAATTGTCTTCAAAA+TGG - chr1_3:5848176-5848195 Msa0099650:CDS 25.0%
! AATTGTCTTCAAAATGGAAT+TGG - chr1_3:5848182-5848201 Msa0099650:CDS 25.0%
!! GCTAGATTAAATGTTAGTTT+CGG - chr1_3:5848637-5848656 Msa0099650:intron 25.0%
! AAGGATGATTTAATGAGATT+TGG - chr1_3:5848673-5848692 Msa0099650:intron 25.0%
!! AATCTGTCTTCAAGTTAATA+AGG + chr1_3:5848723-5848742 None:intergenic 25.0%
!!! TGGTTTTAGTTCTAACAATA+GGG - chr1_3:5848765-5848784 Msa0099650:intron 25.0%
! TAATGTGGATGAGATTATTA+AGG - chr1_3:5849083-5849102 Msa0099650:CDS 25.0%
!! TTTAAGACAGTTTGAAAATG+AGG - chr1_3:5849455-5849474 Msa0099650:CDS 25.0%
!!! TTTTGATTTTAATGCGAATG+AGG - chr1_3:5849500-5849519 Msa0099650:CDS 25.0%
! CTGATAAATTAATGCAACAA+TGG - chr1_3:5849679-5849698 Msa0099650:CDS 25.0%
! GATAAATTAATGCAACAATG+GGG - chr1_3:5849681-5849700 Msa0099650:CDS 25.0%
! TCAAATGCTTGGAATAAAAA+AGG + chr1_3:5850190-5850209 None:intergenic 25.0%
! ACAGAAATAAATCAAATGCT+TGG + chr1_3:5850201-5850220 None:intergenic 25.0%
! TCAATCTTGTTAATAGTCAT+AGG + chr1_3:5850355-5850374 None:intergenic 25.0%
!!! AGAATTATATAATGATGGGT+TGG - chr1_3:5850591-5850610 Msa0099650:CDS 25.0%
!!! GAATTATATAATGATGGGTT+GGG - chr1_3:5850592-5850611 Msa0099650:CDS 25.0%
!! TTGATTTTGAATCTAGATTG+AGG + chr1_3:5850838-5850857 None:intergenic 25.0%
! TCAATCTAGATTCAAAATCA+AGG - chr1_3:5850837-5850856 Msa0099650:CDS 25.0%
! ATCTTGATTCAACAATTTGT+TGG + chr1_3:5851037-5851056 None:intergenic 25.0%
!! TATTGTATTTTATGGCCTAT+TGG - chr1_3:5851252-5851271 Msa0099650:five_prime_UTR 25.0%
AAAGGAATATTGTGTGGAAA+TGG + chr1_3:5847640-5847659 None:intergenic 30.0%
AGAGAAAAAGGAATATTGTG+TGG + chr1_3:5847646-5847665 None:intergenic 30.0%
ACTTACCAAAGAAGAGAAAA+AGG + chr1_3:5847658-5847677 None:intergenic 30.0%
ATTTGAAAGCAACAAAGGAA+AGG + chr1_3:5847705-5847724 None:intergenic 30.0%
!! GATTGATTTGAAAGCAACAA+AGG + chr1_3:5847710-5847729 None:intergenic 30.0%
TAAAAGACAGCAAAACAACA+TGG + chr1_3:5847820-5847839 None:intergenic 30.0%
GAGTCTGAAAATAAAGATGT+TGG - chr1_3:5847965-5847984 Msa0099650:CDS 30.0%
ATTAAGCAAAGCTCTTTACT+TGG - chr1_3:5848045-5848064 Msa0099650:CDS 30.0%
AACTTTGGTACATAGATGTT+CGG - chr1_3:5848399-5848418 Msa0099650:CDS 30.0%
TTGATTTATGCTTCTGTTGT+TGG - chr1_3:5848478-5848497 Msa0099650:CDS 30.0%
TGATTTATGCTTCTGTTGTT+GGG - chr1_3:5848479-5848498 Msa0099650:CDS 30.0%
ACTGAGTTTAGAAGAGTTAT+GGG - chr1_3:5848555-5848574 Msa0099650:CDS 30.0%
TTTCGGTTATCAAGTAATGA+AGG - chr1_3:5848654-5848673 Msa0099650:intron 30.0%
!!! CTGGTTTTAGTTCTAACAAT+AGG - chr1_3:5848764-5848783 Msa0099650:intron 30.0%
CTATATCAAAAGCTTGATGA+GGG - chr1_3:5848883-5848902 Msa0099650:CDS 30.0%
TATATCAAAAGCTTGATGAG+GGG - chr1_3:5848884-5848903 Msa0099650:CDS 30.0%
! AATTTTCCATCAGTGAACAA+GGG - chr1_3:5848980-5848999 Msa0099650:CDS 30.0%
CACATTAATGATTTCAACTG+TGG + chr1_3:5849070-5849089 None:intergenic 30.0%
CACAGTTGAAATCATTAATG+TGG - chr1_3:5849068-5849087 Msa0099650:CDS 30.0%
ATTCACATTCCTAGAACATA+CGG + chr1_3:5849157-5849176 None:intergenic 30.0%
TACACTTGAACATAACTATG+AGG - chr1_3:5849539-5849558 Msa0099650:CDS 30.0%
ATCAATCATTAATGCAGAGA+AGG - chr1_3:5849628-5849647 Msa0099650:CDS 30.0%
! CTCAAGATCTTCAAGAATTT+TGG + chr1_3:5849658-5849677 None:intergenic 30.0%
CTTCATCGGATATTTCAATT+GGG + chr1_3:5849764-5849783 None:intergenic 30.0%
TCTTCATCGGATATTTCAAT+TGG + chr1_3:5849765-5849784 None:intergenic 30.0%
! ATTCTTAGCATTTCTGAAGA+GGG + chr1_3:5849874-5849893 None:intergenic 30.0%
! AATTCTTAGCATTTCTGAAG+AGG + chr1_3:5849875-5849894 None:intergenic 30.0%
!!! ATATCGTTACCTATTTTTGC+AGG + chr1_3:5849939-5849958 None:intergenic 30.0%
ATTGGATATACTTCTGTACA+TGG - chr1_3:5849959-5849978 Msa0099650:CDS 30.0%
ATCCATTAAGCATATTGTGT+CGG - chr1_3:5850052-5850071 Msa0099650:CDS 30.0%
TCCATGAATAATTCTGTTTC+AGG - chr1_3:5850230-5850249 Msa0099650:CDS 30.0%
TCCTGAAACAGAATTATTCA+TGG + chr1_3:5850234-5850253 None:intergenic 30.0%
TGAATAATTCTGTTTCAGGA+AGG - chr1_3:5850234-5850253 Msa0099650:CDS 30.0%
GAATAATTCTGTTTCAGGAA+GGG - chr1_3:5850235-5850254 Msa0099650:CDS 30.0%
AACAGATAAAGACAAATGCT+TGG - chr1_3:5850303-5850322 Msa0099650:CDS 30.0%
ACTGATAAGGTGAAAATAGA+TGG - chr1_3:5850622-5850641 Msa0099650:CDS 30.0%
! ACTGGTTCATAATTTCTTAG+AGG + chr1_3:5850709-5850728 None:intergenic 30.0%
CTAAGAAATTATGAACCAGT+TGG - chr1_3:5850709-5850728 Msa0099650:CDS 30.0%
AAGAGTTGACATACATGAAA+TGG - chr1_3:5850762-5850781 Msa0099650:CDS 30.0%
! ATTTTGAATCTAGATTGAGG+TGG + chr1_3:5850835-5850854 None:intergenic 30.0%
TGTTGAATCAAGATATCTTG+TGG - chr1_3:5851043-5851062 Msa0099650:five_prime_UTR 30.0%
CATGGATAGATTTCCTAAAT+TGG + chr1_3:5851178-5851197 None:intergenic 30.0%
AAGAAAAGATCATGTCCAAT+AGG + chr1_3:5851270-5851289 None:intergenic 30.0%
AGCAACAAAGGAAAGGAAAA+GGG + chr1_3:5847698-5847717 None:intergenic 35.0%
AAGCAACAAAGGAAAGGAAA+AGG + chr1_3:5847699-5847718 None:intergenic 35.0%
AACATGGTTATCATGTCATG+TGG + chr1_3:5847804-5847823 None:intergenic 35.0%
AGATGGTAGAAGGCTTAAAA+GGG + chr1_3:5848078-5848097 None:intergenic 35.0%
AGATCCTCACTTACAAATCT+CGG + chr1_3:5848145-5848164 None:intergenic 35.0%
TAAGTGAGGATCTGTTGATA+GGG - chr1_3:5848152-5848171 Msa0099650:CDS 35.0%
TCTTCATGTTCATTCGATTG+AGG - chr1_3:5848264-5848283 Msa0099650:CDS 35.0%
CTTCATGTTCATTCGATTGA+GGG - chr1_3:5848265-5848284 Msa0099650:CDS 35.0%
CTAACACCATTGAAACTCAA+AGG + chr1_3:5848295-5848314 None:intergenic 35.0%
! CTTTGAGTTTCAATGGTGTT+AGG - chr1_3:5848293-5848312 Msa0099650:CDS 35.0%
GTACATAGATGTTCGGTTTA+TGG - chr1_3:5848406-5848425 Msa0099650:CDS 35.0%
AGCATAAATCAAGAATCGCT+TGG + chr1_3:5848470-5848489 None:intergenic 35.0%
GATTTATGCTTCTGTTGTTG+GGG - chr1_3:5848480-5848499 Msa0099650:CDS 35.0%
CATAACTCTTCTAAACTCAG+TGG + chr1_3:5848556-5848575 None:intergenic 35.0%
CACTGAGTTTAGAAGAGTTA+TGG - chr1_3:5848554-5848573 Msa0099650:CDS 35.0%
CTGAGTTTAGAAGAGTTATG+GGG - chr1_3:5848556-5848575 Msa0099650:CDS 35.0%
TTATGGGGTGATAAAAGTTC+AGG - chr1_3:5848571-5848590 Msa0099650:CDS 35.0%
TATGGGGTGATAAAAGTTCA+GGG - chr1_3:5848572-5848591 Msa0099650:CDS 35.0%
GTGATAAAAGTTCAGGGAAA+TGG - chr1_3:5848578-5848597 Msa0099650:CDS 35.0%
! GGAAATGGAGTACAAGTTTT+AGG - chr1_3:5848593-5848612 Msa0099650:CDS 35.0%
!!! ATGGAGTACAAGTTTTAGGT+TGG - chr1_3:5848597-5848616 Msa0099650:CDS 35.0%
!!! AGTACAAGTTTTAGGTTGGT+TGG - chr1_3:5848601-5848620 Msa0099650:CDS 35.0%
!! ATGAGATTTGGTGCTAGTAA+TGG - chr1_3:5848685-5848704 Msa0099650:intron 35.0%
!! TGAGATTTGGTGCTAGTAAT+GGG - chr1_3:5848686-5848705 Msa0099650:intron 35.0%
ACTAAAACCAGCAACATTGT+CGG + chr1_3:5848755-5848774 None:intergenic 35.0%
AATGAAGCAGTGATGAATTC+TGG - chr1_3:5848832-5848851 Msa0099650:CDS 35.0%
! CCTCATCAAGCTTTTGATAT+AGG + chr1_3:5848885-5848904 None:intergenic 35.0%
CCTATATCAAAAGCTTGATG+AGG - chr1_3:5848882-5848901 Msa0099650:CDS 35.0%
GGAACTTCAATAATTCAGCA+CGG - chr1_3:5848905-5848924 Msa0099650:CDS 35.0%
GAACTTCAATAATTCAGCAC+GGG - chr1_3:5848906-5848925 Msa0099650:CDS 35.0%
! GAATTTTCCATCAGTGAACA+AGG - chr1_3:5848979-5848998 Msa0099650:CDS 35.0%
! ATTTTCCATCAGTGAACAAG+GGG - chr1_3:5848981-5849000 Msa0099650:CDS 35.0%
TCGTTCGAATTTGATCAAAC+AGG - chr1_3:5849024-5849043 Msa0099650:CDS 35.0%
!! ATGTTCTAGGAATGTGAATG+TGG - chr1_3:5849158-5849177 Msa0099650:CDS 35.0%
TAGGAATGTGAATGTGGATA+TGG - chr1_3:5849164-5849183 Msa0099650:CDS 35.0%
AACTGAATCCTCAATCTTCA+TGG + chr1_3:5849238-5849257 None:intergenic 35.0%
CACTTAAGTAGTGTTCTTCT+TGG + chr1_3:5849284-5849303 None:intergenic 35.0%
ACATAAAAAGCCACATAGCT+TGG - chr1_3:5849323-5849342 Msa0099650:CDS 35.0%
TGTCTTAAAAGCTTCTCTCT+AGG + chr1_3:5849444-5849463 None:intergenic 35.0%
!! TGATTTTAATGCGAATGAGG+AGG - chr1_3:5849503-5849522 Msa0099650:CDS 35.0%
!! GATTTTAATGCGAATGAGGA+GGG - chr1_3:5849504-5849523 Msa0099650:CDS 35.0%
TAACTATGAGGATTATGACG+TGG - chr1_3:5849551-5849570 Msa0099650:CDS 35.0%
!! ATCTTTAATTATTGGTGCCG+CGG - chr1_3:5849584-5849603 Msa0099650:CDS 35.0%
ATCATTAATGCAGAGAAGGA+AGG - chr1_3:5849632-5849651 Msa0099650:CDS 35.0%
TTGAAAATTCTCCAAGAACC+TGG - chr1_3:5849721-5849740 Msa0099650:CDS 35.0%
TAATATGGACGGTTCTTCAT+CGG + chr1_3:5849778-5849797 None:intergenic 35.0%
TTCACCAATCGAAGGTAATA+TGG + chr1_3:5849793-5849812 None:intergenic 35.0%
ATATTACCTTCGATTGGTGA+AGG - chr1_3:5849792-5849811 Msa0099650:CDS 35.0%
TATTACCTTCGATTGGTGAA+GGG - chr1_3:5849793-5849812 Msa0099650:CDS 35.0%
TCGTTTGTTCAAACTATGAG+CGG - chr1_3:5849822-5849841 Msa0099650:CDS 35.0%
TTTCCAGTGATATCTAGCAA+AGG + chr1_3:5850032-5850051 None:intergenic 35.0%
ATCCGACACAATATGCTTAA+TGG + chr1_3:5850057-5850076 None:intergenic 35.0%
CATCCAAGGAGAACATAATT+TGG - chr1_3:5850525-5850544 Msa0099650:CDS 35.0%
GTTCCAAATTATGTTCTCCT+TGG + chr1_3:5850531-5850550 None:intergenic 35.0%
!! TATAATGATGGGTTGGGAAA+TGG - chr1_3:5850598-5850617 Msa0099650:CDS 35.0%
ATCAAGGAGATTCATATTGC+AGG - chr1_3:5850853-5850872 Msa0099650:CDS 35.0%
TAGAGACTAATGCACACATT+AGG - chr1_3:5851091-5851110 Msa0099650:five_prime_UTR 35.0%
!!! CTTGCTGTTTTAGCCAATTT+AGG - chr1_3:5851162-5851181 Msa0099650:five_prime_UTR 35.0%
GAGTACAAATCTACAGAATC+GGG + chr1_3:5851308-5851327 None:intergenic 35.0%
GGAGTACAAATCTACAGAAT+CGG + chr1_3:5851309-5851328 None:intergenic 35.0%
!! GTAGTTGGTGAGATTTGAGA+AGG + chr1_3:5847602-5847621 None:intergenic 40.0%
AGGAAAAGGGTGATTGGAAA+TGG + chr1_3:5847685-5847704 None:intergenic 40.0%
AAGGAAAGGAAAAGGGTGAT+TGG + chr1_3:5847691-5847710 None:intergenic 40.0%
GATGGTAGAAGGCTTAAAAG+GGG + chr1_3:5848077-5848096 None:intergenic 40.0%
GAGATGGTAGAAGGCTTAAA+AGG + chr1_3:5848079-5848098 None:intergenic 40.0%
! TTTTCAGCAGAGATGGTAGA+AGG + chr1_3:5848088-5848107 None:intergenic 40.0%
AAACCCGAGATTTGTAAGTG+AGG - chr1_3:5848138-5848157 Msa0099650:CDS 40.0%
GATCCTCACTTACAAATCTC+GGG + chr1_3:5848144-5848163 None:intergenic 40.0%
GTAAGTGAGGATCTGTTGAT+AGG - chr1_3:5848151-5848170 Msa0099650:CDS 40.0%
! AAGTGAGGATCTGTTGATAG+GGG - chr1_3:5848153-5848172 Msa0099650:CDS 40.0%
! GGACTTCCTTTGAGTTTCAA+TGG - chr1_3:5848286-5848305 Msa0099650:CDS 40.0%
GTGTTAGGTTAAGTGTGCAT+TGG - chr1_3:5848308-5848327 Msa0099650:CDS 40.0%
GGTTAAGTGTGCATTGGAAA+AGG - chr1_3:5848314-5848333 Msa0099650:CDS 40.0%
TGCGGAGTTTGATGAAACTT+TGG - chr1_3:5848384-5848403 Msa0099650:CDS 40.0%
CATAGATGTTCGGTTTATGG+TGG - chr1_3:5848409-5848428 Msa0099650:CDS 40.0%
TGTTTCCCAATGTCATGTTC+AGG + chr1_3:5848508-5848527 None:intergenic 40.0%
ACATGACATTGGGAAACATC+AGG - chr1_3:5848510-5848529 Msa0099650:CDS 40.0%
!! AACATCAGGTTGATCTCACT+AGG - chr1_3:5848524-5848543 Msa0099650:CDS 40.0%
! AGGTTGGTTGGAAAAGCTTT+AGG - chr1_3:5848613-5848632 Msa0099650:CDS 40.0%
TAAAACCAGCAACATTGTCG+GGG + chr1_3:5848753-5848772 None:intergenic 40.0%
CTAAAACCAGCAACATTGTC+GGG + chr1_3:5848754-5848773 None:intergenic 40.0%
ATAGGGATGTCGTAAAGCTT+CGG - chr1_3:5848782-5848801 Msa0099650:intron 40.0%
TAACGCAAGATGTGATTCAC+TGG - chr1_3:5849128-5849147 Msa0099650:CDS 40.0%
TCTACCATCCATGAAGATTG+AGG - chr1_3:5849227-5849246 Msa0099650:CDS 40.0%
GAATCCTCAATCTTCATGGA+TGG + chr1_3:5849234-5849253 None:intergenic 40.0%
ATGACATCATCCAAGCTATG+TGG + chr1_3:5849336-5849355 None:intergenic 40.0%
GAGTGATTTCTTAAAGACGC+TGG - chr1_3:5849368-5849387 Msa0099650:CDS 40.0%
TTTCTTAAAGACGCTGGCAT+TGG - chr1_3:5849374-5849393 Msa0099650:CDS 40.0%
AACAATGGGGTTTAGATGAG+AGG - chr1_3:5849694-5849713 Msa0099650:CDS 40.0%
ACAATGGGGTTTAGATGAGA+GGG - chr1_3:5849695-5849714 Msa0099650:CDS 40.0%
CCAATCGAAGGTAATATGGA+CGG + chr1_3:5849789-5849808 None:intergenic 40.0%
TTTGTTCAAACTATGAGCGG+AGG - chr1_3:5849825-5849844 Msa0099650:CDS 40.0%
! TTAGCATTTCTGAAGAGGGA+AGG + chr1_3:5849870-5849889 None:intergenic 40.0%
GATTAGAAGCCTGAATGATG+AGG + chr1_3:5849905-5849924 None:intergenic 40.0%
! ATTTTTGCAGGTAGAACGAC+TGG + chr1_3:5849927-5849946 None:intergenic 40.0%
GTCGTTCTACCTGCAAAAAT+AGG - chr1_3:5849927-5849946 Msa0099650:CDS 40.0%
ATGCCTTTGCTAGATATCAC+TGG - chr1_3:5850026-5850045 Msa0099650:CDS 40.0%
AAGACTAACAAGGAAGCTTC+TGG - chr1_3:5850080-5850099 Msa0099650:CDS 40.0%
GTCTAGCATGTCAAATGAAG+AGG - chr1_3:5850405-5850424 Msa0099650:CDS 40.0%
ATTTCTCCACGAATGCATGA+TGG + chr1_3:5850433-5850452 None:intergenic 40.0%
GAAATCAACGACGATCTTGA+TGG - chr1_3:5850448-5850467 Msa0099650:CDS 40.0%
TGTCAGTAACGTTGGATCAA+TGG - chr1_3:5850482-5850501 Msa0099650:CDS 40.0%
CAATGGTTGAGACTTGATTC+TGG - chr1_3:5850499-5850518 Msa0099650:CDS 40.0%
!!! TCCAATACTGGTTTTAGCTC+AGG - chr1_3:5850735-5850754 Msa0099650:CDS 40.0%
!!! GGTTACCACCATTTTTGGTA+AGG + chr1_3:5850882-5850901 None:intergenic 40.0%
!! TTGTCGGTTACCACCATTTT+TGG + chr1_3:5850887-5850906 None:intergenic 40.0%
GACAATATTACACATGCCAC+TGG + chr1_3:5850959-5850978 None:intergenic 40.0%
! CACTTTGGGAACATGATATC+AGG + chr1_3:5851120-5851139 None:intergenic 40.0%
GCTCTGACATTGTTCACTTT+GGG + chr1_3:5851134-5851153 None:intergenic 40.0%
TGCTCTGACATTGTTCACTT+TGG + chr1_3:5851135-5851154 None:intergenic 40.0%
CAAATCTACAGAATCGGGAA+GGG + chr1_3:5851303-5851322 None:intergenic 40.0%
ACAAATCTACAGAATCGGGA+AGG + chr1_3:5851304-5851323 None:intergenic 40.0%
TGAGATTTGAGAAGGTGGAG+TGG + chr1_3:5847594-5847613 None:intergenic 45.0%
! GTTGGTGAGATTTGAGAAGG+TGG + chr1_3:5847599-5847618 None:intergenic 45.0%
!!! ACGGTCTTTTTCAGCAGAGA+TGG + chr1_3:5848095-5848114 None:intergenic 45.0%
! TTTTGCAGACTTGCCCATCA+AGG - chr1_3:5848347-5848366 Msa0099650:CDS 45.0%
TTTGCAGACTTGCCCATCAA+GGG - chr1_3:5848348-5848367 Msa0099650:CDS 45.0%
AACCGTCCAATACCCTTGAT+GGG + chr1_3:5848363-5848382 None:intergenic 45.0%
GGGAACCTGAACATGACATT+GGG - chr1_3:5848500-5848519 Msa0099650:CDS 45.0%
AGCACCACATCATTCTGAGT+CGG + chr1_3:5848808-5848827 None:intergenic 45.0%
!! GCAGTGATGAATTCTGGTTC+AGG - chr1_3:5848838-5848857 Msa0099650:CDS 45.0%
TTCTACCCCTTGTTCACTGA+TGG + chr1_3:5848989-5849008 None:intergenic 45.0%
AGGGGTAGAAACATCTGAAG+AGG - chr1_3:5848999-5849018 Msa0099650:CDS 45.0%
CACTGGATACCGTATGTTCT+AGG - chr1_3:5849145-5849164 Msa0099650:CDS 45.0%
GTCAAGAAACTCTGACGTCT+CGG + chr1_3:5849262-5849281 None:intergenic 45.0%
TCATTTCGCTCAAGCTGTGA+CGG - chr1_3:5849405-5849424 Msa0099650:CDS 45.0%
! AGCTTCTCTCTAGGAGACAT+GGG + chr1_3:5849435-5849454 None:intergenic 45.0%
! AAGCTTCTCTCTAGGAGACA+TGG + chr1_3:5849436-5849455 None:intergenic 45.0%
! GAAAATGAGGCTCTAGCTTC+AGG - chr1_3:5849468-5849487 Msa0099650:CDS 45.0%
TAATGCGAATGAGGAGGGAT+TGG - chr1_3:5849509-5849528 Msa0099650:CDS 45.0%
AATGCGAATGAGGAGGGATT+GGG - chr1_3:5849510-5849529 Msa0099650:CDS 45.0%
AATTCTCCAAGAACCTGGTC+TGG - chr1_3:5849726-5849745 Msa0099650:CDS 45.0%
CCGTCCATATTACCTTCGAT+TGG - chr1_3:5849786-5849805 Msa0099650:CDS 45.0%
GTCGGATGCTAAGACTAACA+AGG - chr1_3:5850070-5850089 Msa0099650:CDS 45.0%
CTAACAAGGAAGCTTCTGGA+AGG - chr1_3:5850084-5850103 Msa0099650:CDS 45.0%
TCTGTTTCAGGAAGGGATCA+TGG - chr1_3:5850242-5850261 Msa0099650:CDS 45.0%
! CGACGATCTTGATGGTTTGA+TGG - chr1_3:5850456-5850475 Msa0099650:CDS 45.0%
GATGGATCTGTCAGTAACGT+TGG - chr1_3:5850474-5850493 Msa0099650:CDS 45.0%
CTTGATTCTGGCATCATCCA+AGG - chr1_3:5850511-5850530 Msa0099650:CDS 45.0%
!! GTTGGGAAATGGCACTGATA+AGG - chr1_3:5850609-5850628 Msa0099650:CDS 45.0%
! TGGCAGAAAGCGCAGTTATT+TGG - chr1_3:5850642-5850661 Msa0099650:CDS 45.0%
! GGCAGAAAGCGCAGTTATTT+GGG - chr1_3:5850643-5850662 Msa0099650:CDS 45.0%
! TTTGGGAGAGCACATAACAG+TGG - chr1_3:5850660-5850679 Msa0099650:CDS 45.0%
TTCTTAGAGGATCTCTGTGC+TGG + chr1_3:5850696-5850715 None:intergenic 45.0%
ACCAGTTGGAGTTCCAATAC+TGG - chr1_3:5850723-5850742 Msa0099650:CDS 45.0%
! ACCAGTATTGGAACTCCAAC+TGG + chr1_3:5850727-5850746 None:intergenic 45.0%
GCCTGAGCTAAAACCAGTAT+TGG + chr1_3:5850739-5850758 None:intergenic 45.0%
GCAGGTGTCCTTACCAAAAA+TGG - chr1_3:5850871-5850890 Msa0099650:CDS 45.0%
GGTGTCCTTACCAAAAATGG+TGG - chr1_3:5850874-5850893 Msa0099650:CDS 45.0%
ATGGTGGTAACCGACAAGTT+TGG - chr1_3:5850890-5850909 Msa0099650:CDS 45.0%
TGGTGGTAACCGACAAGTTT+GGG - chr1_3:5850891-5850910 Msa0099650:CDS 45.0%
! GACTTGCCCATCAAGGGTAT+TGG - chr1_3:5848354-5848373 Msa0099650:CDS 50.0%
!! TGCCCATCAAGGGTATTGGA+CGG - chr1_3:5848358-5848377 Msa0099650:CDS 50.0%
GAACCGTCCAATACCCTTGA+TGG + chr1_3:5848364-5848383 None:intergenic 50.0%
!! AAGGGTATTGGACGGTTCTG+CGG - chr1_3:5848366-5848385 Msa0099650:CDS 50.0%
GGGGAACCTGAACATGACAT+TGG - chr1_3:5848499-5848518 Msa0099650:CDS 50.0%
AACATCCCCGACAATGTTGC+TGG - chr1_3:5848745-5848764 Msa0099650:intron 50.0%
TAATTCAGCACGGGCAGACT+CGG - chr1_3:5848915-5848934 Msa0099650:CDS 50.0%
ATGCGAATGAGGAGGGATTG+GGG - chr1_3:5849511-5849530 Msa0099650:CDS 50.0%
!! AATTATTGGTGCCGCGGAAG+AGG - chr1_3:5849590-5849609 Msa0099650:CDS 50.0%
CCTTTCATACTCCTCTTCCG+CGG + chr1_3:5849604-5849623 None:intergenic 50.0%
! CGCGGAAGAGGAGTATGAAA+GGG - chr1_3:5849602-5849621 Msa0099650:CDS 50.0%
AATCCACCAGACCAGGTTCT+TGG + chr1_3:5849735-5849754 None:intergenic 50.0%
!! AGAACCTGGTCTGGTGGATT+TGG - chr1_3:5849735-5849754 Msa0099650:CDS 50.0%
! CTATGAGCGGAGGCTTTTTG+AGG - chr1_3:5849835-5849854 Msa0099650:CDS 50.0%
TCTGAAGAGGGAAGGACACA+TGG + chr1_3:5849862-5849881 None:intergenic 50.0%
TTGTGCGAGCCTCATCATTC+AGG - chr1_3:5849893-5849912 Msa0099650:CDS 50.0%
GGTGGTAACCGACAAGTTTG+GGG - chr1_3:5850892-5850911 Msa0099650:CDS 50.0%
ACGCAGTTCCCCAAACTTGT+CGG + chr1_3:5850903-5850922 None:intergenic 50.0%
ACTGCGTCTCAGCAACAATC+CGG - chr1_3:5850916-5850935 Msa0099650:CDS 50.0%
!! TTGCGTTGGTTGCTTTCCAG+TGG - chr1_3:5850940-5850959 Msa0099650:exon 50.0%
! TCGGCCGACTCAGAATGATG+TGG - chr1_3:5848801-5848820 Msa0099650:CDS 55.0%
GCAGACTCGGAGTCATCTCA+AGG - chr1_3:5848928-5848947 Msa0099650:CDS 55.0%
! CCGCGGAAGAGGAGTATGAA+AGG - chr1_3:5849601-5849620 Msa0099650:CDS 55.0%
TCTCCAAGAACCTGGTCTGG+TGG - chr1_3:5849729-5849748 Msa0099650:CDS 55.0%
GCTTCCAAATCCACCAGACC+AGG + chr1_3:5849742-5849761 None:intergenic 55.0%
CCAAGCCCTTCACCAATCGA+AGG + chr1_3:5849801-5849820 None:intergenic 55.0%
CCTTCGATTGGTGAAGGGCT+TGG - chr1_3:5849798-5849817 Msa0099650:CDS 55.0%
! GAGGCACCATCATGCATTCG+TGG - chr1_3:5850424-5850443 Msa0099650:CDS 55.0%
CTGCGTCTCAGCAACAATCC+GGG - chr1_3:5850917-5850936 Msa0099650:CDS 55.0%
AGCAACAATCCGGGTTGCGT+TGG - chr1_3:5850926-5850945 Msa0099650:CDS 55.0%
GGAAAGCAACCAACGCAACC+CGG + chr1_3:5850938-5850957 None:intergenic 55.0%
TATGGTGGTCGCGTCGTGTC+TGG - chr1_3:5848424-5848443 Msa0099650:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 5847560 5851329 5847560 ID=Msa0099650;Name=Msa0099650
chr1_3 mRNA 5847560 5851329 5847560 ID=Msa0099650-mRNA-1;Parent=Msa0099650;Name=Msa0099650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=384|1|1|1|1|1|2|189|1016
chr1_3 exon 5847560 5848637 5847560 ID=Msa0099650-mRNA-1:exon:2252;Parent=Msa0099650-mRNA-1
chr1_3 exon 5848784 5851329 5848784 ID=Msa0099650-mRNA-1:exon:2251;Parent=Msa0099650-mRNA-1
chr1_3 five_prime_UTR 5850946 5851329 5850946 ID=Msa0099650-mRNA-1:five_prime_utr;Parent=Msa0099650-mRNA-1
chr1_3 CDS 5848784 5850945 5848784 ID=Msa0099650-mRNA-1:cds;Parent=Msa0099650-mRNA-1
chr1_3 CDS 5847749 5848637 5847749 ID=Msa0099650-mRNA-1:cds;Parent=Msa0099650-mRNA-1
chr1_3 three_prime_UTR 5847560 5847748 5847560 ID=Msa0099650-mRNA-1:three_prime_utr;Parent=Msa0099650-mRNA-1
Gene Sequence

>Msa0099650

ATGATGATGAAACCAAACTTTGAGTCTGAAAATAAAGATGTTGGTGAAACTGAAACTGATTCAAATATTGGTCATGAACAATTACTTCGTGACATTGAAGAATTAAGCAAAGCTCTTTACTTGGACAATACCCCTTTTAAGCCTTCTACCATCTCTGCTGAAAAAGACCGTTTGTCAAAATCTCAACTTAACTCAAACCCGAGATTTGTAAGTGAGGATCTGTTGATAGGGGACAAGAAATTGTCTTCAAAATGGAATTGGAAGAAGCCCTTGAAAGTTTTAACAAATATTGGTTCACAAAAGTTTGTTTGTTGCTTTAATCTTCATGTTCATTCGATTGAGGGACTTCCTTTGAGTTTCAATGGTGTTAGGTTAAGTGTGCATTGGAAAAGGAAAAACAGCATTTTGCAGACTTGCCCATCAAGGGTATTGGACGGTTCTGCGGAGTTTGATGAAACTTTGGTACATAGATGTTCGGTTTATGGTGGTCGCGTCGTGTCTGGTCGTTCTGTTAAGTATGAATCCAAGCGATTCTTGATTTATGCTTCTGTTGTTGGGGAACCTGAACATGACATTGGGAAACATCAGGTTGATCTCACTAGGCTTTTGCCACTGAGTTTAGAAGAGTTATGGGGTGATAAAAGTTCAGGGAAATGGAGTACAAGTTTTAGGTTGGTTGGAAAAGCTTTAGGAGCTAGATTAAATGTTAGTTTCGGTTATCAAGTAATGAAGGATGATTTAATGAGATTTGGTGCTAGTAATGGGAATGTTGTTAACCTTATTAACTTGAAGACAGATTTAAACATCCCCGACAATGTTGCTGGTTTTAGTTCTAACAATAGGGATGTCGTAAAGCTTCGGCCGACTCAGAATGATGTGGTGCTATCGAATGAAGCAGTGATGAATTCTGGTTCAGGTTTTTCTAAATCAATCACTTTCCTATATCAAAAGCTTGATGAGGGGAACTTCAATAATTCAGCACGGGCAGACTCGGAGTCATCTCAAGGATCAAATCTATATGTGTCTGATGACACTGAATTTTCCATCAGTGAACAAGGGGTAGAAACATCTGAAGAGGATTCGTTCGAATTTGATCAAACAGGAATTCAAATAATTGACATGTCCACAGTTGAAATCATTAATGTGGATGAGATTATTAAGGATGATGACACGTTTGCTGACAATAACGCAAGATGTGATTCACTGGATACCGTATGTTCTAGGAATGTGAATGTGGATATGGCAGATAACAGCAAACATCGATTCAGTATTTCATGTGTCGATCTACCATCCATGAAGATTGAGGATTCAGTTTCCGAGACGTCAGAGTTTCTTGACCAAGAAGAACACTACTTAAGTGTTAAATCAAATTATAAAGCACATAAAAAGCCACATAGCTTGGATGATGTCATCGACTCTGTCGCGAGTGATTTCTTAAAGACGCTGGCATTGGAGAGTGATTCATTTCGCTCAAGCTGTGACGGTGATCCCATGTCTCCTAGAGAGAAGCTTTTAAGACAGTTTGAAAATGAGGCTCTAGCTTCAGGCAACTTCGCTTTTGATTTTAATGCGAATGAGGAGGGATTGGGGCAATATACACTTGAACATAACTATGAGGATTATGACGTGGATTCTGATTTATCTTTAATTATTGGTGCCGCGGAAGAGGAGTATGAAAGGGAAAATCAATCATTAATGCAGAGAAGGAAGGCCAAAATTCTTGAAGATCTTGAGACTGATAAATTAATGCAACAATGGGGTTTAGATGAGAGGGACTTTGAAAATTCTCCAAGAACCTGGTCTGGTGGATTTGGAAGCCCAATTGAAATATCCGATGAAGAACCGTCCATATTACCTTCGATTGGTGAAGGGCTTGGTTCGTTTGTTCAAACTATGAGCGGAGGCTTTTTGAGGTCCATGTGTCCTTCCCTCTTCAGAAATGCTAAGAATTGTGCGAGCCTCATCATTCAGGCTTCTAATCCAGTCGTTCTACCTGCAAAAATAGGTAACGATATATTGGATATACTTCTGTACATGGCATCTGCTAGAGTTGAAGAGCTGTGCAATTATATCTCTAAATCGATGCCTTTGCTAGATATCACTGGAAAATCCATTAAGCATATTGTGTCGGATGCTAAGACTAACAAGGAAGCTTCTGGAAGGAAGGGATCATGGCAGCATGATTTGTTTGAAGAATTTCCATGTAGTTATTTAACAGATAAAGACAAATGCTTGGACTCTTTGTCTCTAGAAGCTATAGCTCCTATGACTATTAACAAGATTGAATCTCTTTTAATAGAAGGGTTGAGAATTCAGTCTAGCATGTCAAATGAAGAGGCACCATCATGCATTCGTGGAGAAATCAACGACGATCTTGATGGTTTGATGGATCTGTCAGTAACGTTGGATCAATGGTTGAGACTTGATTCTGGCATCATCCAAGGAGAACATAATTTGGAACAAATTTTGAAGATTCTTAAAGCACACAATTCTAAAATCACAGAATTATATAATGATGGGTTGGGAAATGGCACTGATAAGGTGAAAATAGATGGCAGAAAGCGCAGTTATTTGGGAGAGCACATAACAGTGGCTTTTATGATCCAGCACAGAGATCCTCTAAGAAATTATGAACCAGTTGGAGTTCCAATACTGGTTTTAGCTCAGGCAGAAAGAGTTGACATACATGAAATGGAGAAAGACTGCGACAATTTTGTTGAGAATGAAGATATAGATAAAGAACCACCTCAATCTAGATTCAAAATCAAGGAGATTCATATTGCAGGTGTCCTTACCAAAAATGGTGGTAACCGACAAGTTTGGGGAACTGCGTCTCAGCAACAATCCGGGTTGCGTTGGTTGCTTTCCAGTGGCATGTGTAATATTGTCAAACACTCTTCTTCAAAATCAAAGTCGATTGTTGTTAGATCATCTTCACTGTTTACCAACAAATTGTTGAATCAAGATATCTTGTGGAGCATCTCTTGTGTTGATAGTAACATAGAGACTAATGCACACATTAGGAATCCTGATATCATGTTCCCAAAGTGA
Protein sequence

>Msa0099650

MMMKPNFESENKDVGETETDSNIGHEQLLRDIEELSKALYLDNTPFKPSTISAEKDRLSKSQLNSNPRFVSEDLLIGDKKLSSKWNWKKPLKVLTNIGSQKFVCCFNLHVHSIEGLPLSFNGVRLSVHWKRKNSILQTCPSRVLDGSAEFDETLVHRCSVYGGRVVSGRSVKYESKRFLIYASVVGEPEHDIGKHQVDLTRLLPLSLEELWGDKSSGKWSTSFRLVGKALGARLNVSFGYQVMKDDLMRFGASNGNVVNLINLKTDLNIPDNVAGFSSNNRDVVKLRPTQNDVVLSNEAVMNSGSGFSKSITFLYQKLDEGNFNNSARADSESSQGSNLYVSDDTEFSISEQGVETSEEDSFEFDQTGIQIIDMSTVEIINVDEIIKDDDTFADNNARCDSLDTVCSRNVNVDMADNSKHRFSISCVDLPSMKIEDSVSETSEFLDQEEHYLSVKSNYKAHKKPHSLDDVIDSVASDFLKTLALESDSFRSSCDGDPMSPREKLLRQFENEALASGNFAFDFNANEEGLGQYTLEHNYEDYDVDSDLSLIIGAAEEEYERENQSLMQRRKAKILEDLETDKLMQQWGLDERDFENSPRTWSGGFGSPIEISDEEPSILPSIGEGLGSFVQTMSGGFLRSMCPSLFRNAKNCASLIIQASNPVVLPAKIGNDILDILLYMASARVEELCNYISKSMPLLDITGKSIKHIVSDAKTNKEASGRKGSWQHDLFEEFPCSYLTDKDKCLDSLSLEAIAPMTINKIESLLIEGLRIQSSMSNEEAPSCIRGEINDDLDGLMDLSVTLDQWLRLDSGIIQGEHNLEQILKILKAHNSKITELYNDGLGNGTDKVKIDGRKRSYLGEHITVAFMIQHRDPLRNYEPVGVPILVLAQAERVDIHEMEKDCDNFVENEDIDKEPPQSRFKIKEIHIAGVLTKNGGNRQVWGTASQQQSGLRWLLSSGMCNIVKHSSSKSKSIVVRSSSLFTNKLLNQDILWSISCVDSNIETNAHIRNPDIMFPK*