AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099620 MtrunA17_Chr1g0150791 89.765 1065 60 1 1 1016 1 1065 0.0 1933
Msa0099620 MtrunA17_Chr2g0300211 34.388 1201 587 31 2 1015 1 1187 4.64e-170 530
Msa0099620 MtrunA17_Chr4g0070741 35.299 1102 563 24 24 1015 9 1070 1.04e-169 525
Msa0099620 MtrunA17_Chr6g0483131 38.835 206 121 3 44 246 78 281 4.34e-34 141
Msa0099620 MtrunA17_Chr6g0483131 27.208 419 215 13 618 1012 514 866 6.25e-27 118
Msa0099620 MtrunA17_Chr8g0392621 36.782 174 104 3 76 246 126 296 8.17e-27 118
Msa0099620 MtrunA17_Chr8g0392621 25.059 427 223 14 618 1016 535 892 1.03e-24 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099620 AT5G26160.3 37.302 1008 526 25 18 994 8 940 1.37e-170 525
Msa0099620 AT5G26160.2 37.302 1008 526 25 18 994 8 940 1.67e-170 525
Msa0099620 AT5G26160.1 37.302 1008 526 25 18 994 8 940 1.67e-170 525
Msa0099620 AT5G20610.1 35.591 635 313 16 464 1016 544 1164 4.39e-92 318
Msa0099620 AT5G20610.1 40.063 317 153 6 26 320 13 314 1.39e-57 217
Msa0099620 AT1G42550.1 37.725 167 98 4 85 246 123 288 5.36e-26 115
Msa0099620 AT1G42550.1 25.478 314 156 12 552 826 458 732 2.87e-12 71.6

Find 218 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGATCTTCAAGAATTT+TGG 0.094286 1_3:+5824978 None:intergenic
ATGAAGTGAATGAATTTATT+AGG 0.170610 1_3:-5826738 Msa0099620:five_prime_UTR
TCTGATTTATCTTTAATTAT+TGG 0.172054 1_3:-5825057 Msa0099620:CDS
TGGCAGAAAGCGCAGTTATT+TGG 0.193805 1_3:-5823991 Msa0099620:CDS
CATGGATAGATTTCCTAAAT+TGG 0.196193 1_3:+5823458 None:intergenic
GTACATAGATGTTCGGTTTA+TGG 0.206716 1_3:-5826227 Msa0099620:CDS
GAGATGGTAGAAGGCTTAAA+AGG 0.248939 1_3:+5826557 None:intergenic
AATCTGTCTTCAAGTTAATA+AGG 0.251956 1_3:+5825913 None:intergenic
CATCCAAGGAGAACATAATT+TGG 0.267826 1_3:-5824108 Msa0099620:CDS
CACTTAAGTAGTGTTCTTCT+TGG 0.277300 1_3:+5825352 None:intergenic
ACTGAAACTGATTCAAATAT+TGG 0.283455 1_3:-5826641 Msa0099620:CDS
CACTTTGGGAACATGATATC+AGG 0.285981 1_3:+5823516 None:intergenic
ATCCGACACAATATGCTTAA+TGG 0.290782 1_3:+5824579 None:intergenic
TGGTGGTAACCGACAAGTTT+GGG 0.293482 1_3:-5823742 Msa0099620:CDS
AGAACCTGGTCTGGTGGATT+TGG 0.298861 1_3:-5824898 Msa0099620:CDS
TTCACCAATCGAAGGTAATA+TGG 0.301986 1_3:+5824843 None:intergenic
TGATTTATGCTTCTGTTGTT+GGG 0.305019 1_3:-5826154 Msa0099620:CDS
TTGTGCGAGCCTCATCATTC+AGG 0.308272 1_3:-5824740 Msa0099620:CDS
AGGTTGGTTGGAAAAGCTTT+AGG 0.311083 1_3:-5826020 Msa0099620:CDS
TTGATTTATGCTTCTGTTGT+TGG 0.316516 1_3:-5826155 Msa0099620:CDS
CACTGAGTTTAGAAGAGTTA+TGG 0.323931 1_3:-5826079 Msa0099620:CDS
TAATGTGGATGAGATTATTA+AGG 0.327179 1_3:-5825550 Msa0099620:CDS
ACTGGTTCATAATTTCTTAG+AGG 0.327756 1_3:+5823927 None:intergenic
ACTTACCAAAGAAGAGAAAA+AGG 0.328492 1_3:+5826976 None:intergenic
CTTTGAGTTTCAATGGTGTT+AGG 0.329725 1_3:-5826340 Msa0099620:CDS
GGCAGAAAGCGCAGTTATTT+GGG 0.332530 1_3:-5823990 Msa0099620:CDS
ACAAGAAATTGTCTTCAAAA+TGG 0.335837 1_3:-5826457 Msa0099620:CDS
CACTGGATACCGTATGTTCT+AGG 0.342638 1_3:-5825488 Msa0099620:CDS
AAGACTAACAAGGAAGCTTC+TGG 0.343748 1_3:-5824553 Msa0099620:CDS
AATTGTCTTCAAAATGGAAT+TGG 0.344941 1_3:-5826451 Msa0099620:CDS
TGTTTCCCAATGTCATGTTC+AGG 0.348192 1_3:+5826128 None:intergenic
GTAAGTGAGGATCTGTTGAT+AGG 0.349589 1_3:-5826482 Msa0099620:CDS
TTATGGGGTGATAAAAGTTC+AGG 0.358120 1_3:-5826062 Msa0099620:CDS
AAGGATGATTTAATGAGATT+TGG 0.360201 1_3:-5825960 Msa0099620:CDS
GCTCTGACATTGTTCACTTT+GGG 0.360351 1_3:+5823502 None:intergenic
TGCTCTGACATTGTTCACTT+TGG 0.361069 1_3:+5823501 None:intergenic
ACCAGTTGGAGTTCCAATAC+TGG 0.365800 1_3:-5823910 Msa0099620:CDS
AATCCACCAGACCAGGTTCT+TGG 0.366360 1_3:+5824901 None:intergenic
TCTTCATCGGATATTTCAAT+TGG 0.366592 1_3:+5824871 None:intergenic
TGAGATTTGGTGCTAGTAAT+GGG 0.369936 1_3:-5825947 Msa0099620:CDS
CGACGATCTTGATGGTTTGA+TGG 0.371384 1_3:-5824177 Msa0099620:CDS
AAAGGAATATTGTGTGGAAA+TGG 0.373622 1_3:+5826994 None:intergenic
CTTCATGTTCATTCGATTGA+GGG 0.378234 1_3:-5826368 Msa0099620:CDS
AATATTTGTTAAAACTTTCA+AGG 0.378277 1_3:+5826421 None:intergenic
AATGAAGCAGTGATGAATTC+TGG 0.379585 1_3:-5825801 Msa0099620:CDS
AACTGAATCCTCAATCTTCA+TGG 0.382045 1_3:+5825398 None:intergenic
TGAAGTGAATGAATTTATTA+GGG 0.382756 1_3:-5826737 Msa0099620:five_prime_UTR
AATGCGAATGAGGAGGGATT+GGG 0.384507 1_3:-5825123 Msa0099620:CDS
ACTGAGTTTAGAAGAGTTAT+GGG 0.386969 1_3:-5826078 Msa0099620:CDS
GAAAATGAGGCTCTAGCTTC+AGG 0.387737 1_3:-5825165 Msa0099620:CDS
AAGAGTTGACATACATGAAA+TGG 0.388521 1_3:-5823871 Msa0099620:CDS
CAATGGTTGAGACTTGATTC+TGG 0.388547 1_3:-5824134 Msa0099620:CDS
CCTTCGATTGGTGAAGGGCT+TGG 0.388984 1_3:-5824835 Msa0099620:CDS
ATGGTGGTAACCGACAAGTT+TGG 0.394268 1_3:-5823743 Msa0099620:CDS
ATCAAGGAGATTCATATTGC+AGG 0.400807 1_3:-5823780 Msa0099620:CDS
GTGATAAAAGTTCAGGGAAA+TGG 0.403771 1_3:-5826055 Msa0099620:CDS
TCAATCTTGTTAATAGTCAT+AGG 0.404954 1_3:+5824281 None:intergenic
TATAATGATGGGTTGGGAAA+TGG 0.405336 1_3:-5824035 Msa0099620:CDS
ACCAGTATTGGAACTCCAAC+TGG 0.408338 1_3:+5823909 None:intergenic
TATGGTGGTCGCGTCGTGTC+TGG 0.409194 1_3:-5826209 Msa0099620:CDS
AAGAAAAGATCATGTCCAAT+AGG 0.410891 1_3:+5823366 None:intergenic
GCTAGATTAAATGTTAGTTT+CGG 0.410989 1_3:-5825996 Msa0099620:CDS
GAACCGTCCAATACCCTTGA+TGG 0.412288 1_3:+5826272 None:intergenic
GGACTTCCTTTGAGTTTCAA+TGG 0.413613 1_3:-5826347 Msa0099620:CDS
TTTAAGACAGTTTGAAAATG+AGG 0.413809 1_3:-5825178 Msa0099620:CDS
TGATAAATTAATGCAACAAT+GGG 0.414227 1_3:-5824953 Msa0099620:CDS
ATCTTGATTCAACAATTTGT+TGG 0.416147 1_3:+5823599 None:intergenic
ATCACAGAATTATATAATGA+TGG 0.420774 1_3:-5824047 Msa0099620:CDS
AAGGAAAGGAAAAGGGTGAT+TGG 0.421702 1_3:+5826943 None:intergenic
ATTAAGCAAAGCTCTTTACT+TGG 0.426857 1_3:-5826588 Msa0099620:CDS
TAATGCGAATGAGGAGGGAT+TGG 0.434699 1_3:-5825124 Msa0099620:CDS
AACTTTGGTACATAGATGTT+CGG 0.436998 1_3:-5826234 Msa0099620:CDS
GGTTAAGTGTGCATTGGAAA+AGG 0.437103 1_3:-5826319 Msa0099620:CDS
CTAAAACAAGCAACATTGTC+GGG 0.437682 1_3:+5825882 None:intergenic
GTTCCAAATTATGTTCTCCT+TGG 0.441194 1_3:+5824105 None:intergenic
AGGAAAAGGGTGATTGGAAA+TGG 0.443271 1_3:+5826949 None:intergenic
TTCTTAGAGGATCTCTGTGC+TGG 0.444411 1_3:+5823940 None:intergenic
GGAGTACAAATCTACAGAAT+CGG 0.444771 1_3:+5823327 None:intergenic
ATGAGATTTGGTGCTAGTAA+TGG 0.445911 1_3:-5825948 Msa0099620:CDS
ACTGCGTCTCAGCAACAATC+CGG 0.449045 1_3:-5823717 Msa0099620:CDS
TTCTACCCCTTGTTCACTGA+TGG 0.449718 1_3:+5825647 None:intergenic
TCGTTCGAATTTGATCAAAC+AGG 0.452174 1_3:-5825609 Msa0099620:CDS
GACTTGCCCATCAAGGGTAT+TGG 0.456422 1_3:-5826279 Msa0099620:CDS
GAAATCAACGACGATCTTGA+TGG 0.458924 1_3:-5824185 Msa0099620:CDS
ACTGATAAGGTGAAAATAGA+TGG 0.459395 1_3:-5824011 Msa0099620:CDS
TAGAGACTAATGCACACATT+AGG 0.459516 1_3:-5823542 Msa0099620:CDS
TAGGAATGTGAATGTGGATA+TGG 0.460785 1_3:-5825469 Msa0099620:CDS
TCTGTTTCAGGAAGGGATCA+TGG 0.464848 1_3:-5824391 Msa0099620:intron
TCTTCATGTTCATTCGATTG+AGG 0.468038 1_3:-5826369 Msa0099620:CDS
AATTCTCCAAGAACCTGGTC+TGG 0.470655 1_3:-5824907 Msa0099620:CDS
GAATTATATAATGATGGGTT+GGG 0.472771 1_3:-5824041 Msa0099620:CDS
TTTCTTAAAGACGCTGGCAT+TGG 0.474864 1_3:-5825259 Msa0099620:CDS
AGATCCTCACTTACAAATCT+CGG 0.474977 1_3:+5826491 None:intergenic
TTTCGGTTATCAAGTAATGA+AGG 0.475758 1_3:-5825979 Msa0099620:CDS
AAGCAACAAAGGAAAGGAAA+AGG 0.477857 1_3:+5826935 None:intergenic
AGAATTATATAATGATGGGT+TGG 0.478189 1_3:-5824042 Msa0099620:CDS
TGCGGAGTTTGATGAAACTT+TGG 0.478584 1_3:-5826249 Msa0099620:CDS
GAGTACAAATCTACAGAATC+GGG 0.479741 1_3:+5823328 None:intergenic
TGTCAGTAACGTTGGATCAA+TGG 0.480802 1_3:-5824151 Msa0099620:CDS
GCCTGAGCTAAAACCAGTAT+TGG 0.481373 1_3:+5823897 None:intergenic
TAAGTGAGGATCTGTTGATA+GGG 0.484135 1_3:-5826481 Msa0099620:CDS
TTGAAAATTCTCCAAGAACC+TGG 0.492352 1_3:-5824912 Msa0099620:CDS
AATTATTGGTGCCGCGGAAG+AGG 0.495603 1_3:-5825043 Msa0099620:CDS
GACAATATTACACATGCCAC+TGG 0.497586 1_3:+5823677 None:intergenic
CTGATAAATTAATGCAACAA+TGG 0.498084 1_3:-5824954 Msa0099620:CDS
GATTTATGCTTCTGTTGTTG+GGG 0.498131 1_3:-5826153 Msa0099620:CDS
GATCCTCACTTACAAATCTC+GGG 0.499409 1_3:+5826492 None:intergenic
AACAGATAAAGACAAATGCT+TGG 0.500593 1_3:-5824330 Msa0099620:CDS
AGCATAAATCAAGAATCGCT+TGG 0.502217 1_3:+5826166 None:intergenic
AGATGGTAGAAGGCTTAAAA+GGG 0.502789 1_3:+5826558 None:intergenic
TCAATCTAGATTCAAAATCA+AGG 0.504472 1_3:-5823796 Msa0099620:CDS
ACGCAGTTCCCCAAACTTGT+CGG 0.504742 1_3:+5823733 None:intergenic
GGGAACCTGAACATGACATT+GGG 0.508776 1_3:-5826133 Msa0099620:CDS
AACAATGGGGTTTAGATGAG+AGG 0.511115 1_3:-5824939 Msa0099620:CDS
GTCAAGAAACTCTGACGTCT+CGG 0.513109 1_3:+5825374 None:intergenic
TAATTCAGCACGGGCAGACT+CGG 0.515926 1_3:-5825718 Msa0099620:CDS
ATCAATCATTAATGCAGAGA+AGG 0.520043 1_3:-5825005 Msa0099620:CDS
ATATTTGTTAAAACTTTCAA+GGG 0.523046 1_3:+5826422 None:intergenic
ATGCCTTTGCTAGATATCAC+TGG 0.527711 1_3:-5824607 Msa0099620:CDS
GTTGGGAAATGGCACTGATA+AGG 0.529156 1_3:-5824024 Msa0099620:CDS
AGCAACAATCCGGGTTGCGT+TGG 0.533759 1_3:-5823707 Msa0099620:CDS
AGCAACAAAGGAAAGGAAAA+GGG 0.534880 1_3:+5826936 None:intergenic
TTATCTGTTAAATAACTACA+TGG 0.535658 1_3:+5824344 None:intergenic
ATAGGGATGTCGTAAAGCTT+CGG 0.535896 1_3:-5825851 Msa0099620:CDS
TCACAGAATTATATAATGAT+GGG 0.538575 1_3:-5824046 Msa0099620:CDS
ATATTACCTTCGATTGGTGA+AGG 0.538823 1_3:-5824841 Msa0099620:CDS
ACATGACATTGGGAAACATC+AGG 0.539471 1_3:-5826123 Msa0099620:CDS
ACTTCCAAATCCACCAGACC+AGG 0.539639 1_3:+5824894 None:intergenic
GCAGTGATGAATTCTGGCTC+AGG 0.540966 1_3:-5825795 Msa0099620:CDS
TAACGCAAGATGTGATTCAC+TGG 0.542746 1_3:-5825505 Msa0099620:CDS
CTAAGAAATTATGAACCAGT+TGG 0.543091 1_3:-5823924 Msa0099620:CDS
TGTCTTAAAAGCTTCTCTCT+AGG 0.544252 1_3:+5825192 None:intergenic
TCTACCATCCATGAAGATTG+AGG 0.546459 1_3:-5825406 Msa0099620:CDS
TGTTGAATCAAGATATCTTG+TGG 0.547330 1_3:-5823590 Msa0099620:CDS
GATGGATCTGTCAGTAACGT+TGG 0.547375 1_3:-5824159 Msa0099620:CDS
TCATTTCGCTCAAGCTGTGA+CGG 0.549382 1_3:-5825228 Msa0099620:CDS
TCTCCAAGAACCTGGTCTGG+TGG 0.549400 1_3:-5824904 Msa0099620:CDS
ACTAAAACAAGCAACATTGT+CGG 0.549458 1_3:+5825881 None:intergenic
TACACTTGAACATAACTATG+AGG 0.551566 1_3:-5825094 Msa0099620:CDS
AAGCTTCTCTCTAGGAGACA+TGG 0.552937 1_3:+5825200 None:intergenic
ATTCTTAGCATTTCTGAAGA+GGG 0.553950 1_3:+5824762 None:intergenic
GTCTAGCATGTCAAATGAAG+AGG 0.553980 1_3:-5824228 Msa0099620:CDS
TTGCGTTGGTTGCTTTCCAG+TGG 0.555258 1_3:-5823693 Msa0099620:CDS
CCTATATCAAAAGCTTGATG+AGG 0.555755 1_3:-5825751 Msa0099620:CDS
CTATATCAAAAGCTTGATGA+GGG 0.556939 1_3:-5825750 Msa0099620:CDS
CTTGATTCTGGCATCATCCA+AGG 0.557881 1_3:-5824122 Msa0099620:CDS
AATTCTTAGCATTTCTGAAG+AGG 0.558323 1_3:+5824761 None:intergenic
TTAGCATTTCTGAAGAGGGA+AGG 0.560033 1_3:+5824766 None:intergenic
GTTGGTGAGATTTGAGAAGG+TGG 0.560894 1_3:+5827035 None:intergenic
TAATATGGACGGTTCTTCAT+CGG 0.561202 1_3:+5824858 None:intergenic
CCGCGGAAGAGGAGTATGAA+AGG 0.563504 1_3:-5825032 Msa0099620:CDS
CGCGGAAGAGGAGTATGAAA+GGG 0.564447 1_3:-5825031 Msa0099620:CDS
ATTTGAAAGCAACAAAGGAA+AGG 0.565310 1_3:+5826929 None:intergenic
CACATTAATGATTTCAACTG+TGG 0.565922 1_3:+5825566 None:intergenic
CTGCGTCTCAGCAACAATCC+GGG 0.567017 1_3:-5823716 Msa0099620:CDS
ATCTTTAATTATTGGTGCCG+CGG 0.568539 1_3:-5825049 Msa0099620:CDS
AGCTTCTCTCTAGGAGACAT+GGG 0.568860 1_3:+5825201 None:intergenic
CTAACACCATTGAAACTCAA+AGG 0.571841 1_3:+5826341 None:intergenic
TTTGCAGACTTGCCCATCAA+GGG 0.571889 1_3:-5826285 Msa0099620:CDS
TATTACCTTCGATTGGTGAA+GGG 0.575420 1_3:-5824840 Msa0099620:CDS
ATTCACATTCCTAGAACATA+CGG 0.576528 1_3:+5825479 None:intergenic
GGGGAACCTGAACATGACAT+TGG 0.579850 1_3:-5826134 Msa0099620:CDS
TATGGGGTGATAAAAGTTCA+GGG 0.580001 1_3:-5826061 Msa0099620:CDS
GTGTTAGGTTAAGTGTGCAT+TGG 0.583787 1_3:-5826325 Msa0099620:CDS
AACCGTCCAATACCCTTGAT+GGG 0.583938 1_3:+5826273 None:intergenic
ATCATTAATGCAGAGAAGGA+AGG 0.585207 1_3:-5825001 Msa0099620:CDS
CCGTCCATATTACCTTCGAT+TGG 0.585444 1_3:-5824847 Msa0099620:CDS
ATCCATTAAGCATATTGTGT+CGG 0.586015 1_3:-5824581 Msa0099620:CDS
TCTGAAGAGGGAAGGACACA+TGG 0.586443 1_3:+5824774 None:intergenic
GATTGATTTGAAAGCAACAA+AGG 0.587578 1_3:+5826924 None:intergenic
ATGCGAATGAGGAGGGATTG+GGG 0.589104 1_3:-5825122 Msa0099620:CDS
TCGTTTGTTCAAACTATGAG+CGG 0.591262 1_3:-5824811 Msa0099620:CDS
ATTGGATATACTTCTGTACA+TGG 0.591836 1_3:-5824674 Msa0099620:CDS
GGAAAGCAACCAACGCAACC+CGG 0.594488 1_3:+5823698 None:intergenic
GAATCCTCAATCTTCATGGA+TGG 0.594679 1_3:+5825402 None:intergenic
ATTTCTCCACGAATGCATGA+TGG 0.595591 1_3:+5824203 None:intergenic
AGGGGTAGAAACATCTGAAG+AGG 0.598445 1_3:-5825634 Msa0099620:CDS
CATAGATGTTCGGTTTATGG+TGG 0.601478 1_3:-5826224 Msa0099620:CDS
GAACTTCAATAATTCAGCAC+GGG 0.606552 1_3:-5825727 Msa0099620:CDS
GAGGCACCATCATGCATTCG+TGG 0.607479 1_3:-5824209 Msa0099620:CDS
ACAATGGGGTTTAGATGAGA+GGG 0.609250 1_3:-5824938 Msa0099620:CDS
ACAAATCTACAGAATCGGGA+AGG 0.611751 1_3:+5823332 None:intergenic
AAGGGTATTGGACGGTTCTG+CGG 0.613657 1_3:-5826267 Msa0099620:CDS
CACAGTTGAAATCATTAATG+TGG 0.613849 1_3:-5825565 Msa0099620:CDS
CAAATCTACAGAATCGGGAA+GGG 0.613898 1_3:+5823333 None:intergenic
GAGTCTGAAAATAAAGATGT+TGG 0.617219 1_3:-5826668 Msa0099620:CDS
TGCCCATCAAGGGTATTGGA+CGG 0.622258 1_3:-5826275 Msa0099620:CDS
GATTAGAAGCCTGAATGATG+AGG 0.627712 1_3:+5824731 None:intergenic
ATGACATCATCCAAGCTATG+TGG 0.628164 1_3:+5825300 None:intergenic
GCAGACTCGGAGTCATCTCA+AGG 0.629555 1_3:-5825705 Msa0099620:CDS
CTAACAAGGAAGCTTCTGGA+AGG 0.630482 1_3:-5824549 Msa0099620:intron
CCAAGCCCTTCACCAATCGA+AGG 0.641764 1_3:+5824835 None:intergenic
GAGTGATTTCTTAAAGACGC+TGG 0.643107 1_3:-5825265 Msa0099620:CDS
TTTCCAGTGATATCTAGCAA+AGG 0.643248 1_3:+5824604 None:intergenic
TTTGTTCAAACTATGAGCGG+AGG 0.644551 1_3:-5824808 Msa0099620:CDS
GGAACTTCAATAATTCAGCA+CGG 0.650508 1_3:-5825728 Msa0099620:CDS
GTGGTTGGTGAGATTTGAGA+AGG 0.651344 1_3:+5827032 None:intergenic
CCAATCGAAGGTAATATGGA+CGG 0.651476 1_3:+5824847 None:intergenic
AAGTGAGGATCTGTTGATAG+GGG 0.655616 1_3:-5826480 Msa0099620:CDS
AACACCACGTCATTCTGAGT+CGG 0.655925 1_3:+5825828 None:intergenic
AACATGGTTATCATGTCATG+TGG 0.657702 1_3:+5826832 None:intergenic
CCTTTCATACTCCTCTTCCG+CGG 0.659309 1_3:+5825032 None:intergenic
AACATCAGGTTGATCTCACT+AGG 0.663911 1_3:-5826109 Msa0099620:CDS
TGAGATTTGAGAAGGTGGAG+TGG 0.668267 1_3:+5827040 None:intergenic
GGTGGTAACCGACAAGTTTG+GGG 0.668885 1_3:-5823741 Msa0099620:CDS
ATGTTCTAGGAATGTGAATG+TGG 0.669689 1_3:-5825475 Msa0099620:CDS
GATGGTAGAAGGCTTAAAAG+GGG 0.670150 1_3:+5826559 None:intergenic
AAACCCGAGATTTGTAAGTG+AGG 0.672556 1_3:-5826495 Msa0099620:CDS
TTTGGGAGAGCACATAACAG+TGG 0.673391 1_3:-5823973 Msa0099620:CDS
CTGAGTTTAGAAGAGTTATG+GGG 0.676392 1_3:-5826077 Msa0099620:CDS
TAAAACAAGCAACATTGTCG+GGG 0.684076 1_3:+5825883 None:intergenic
TATATCAAAAGCTTGATGAG+GGG 0.686474 1_3:-5825749 Msa0099620:CDS
TAAAAGACAGCAAAACAACA+TGG 0.692020 1_3:+5826816 None:intergenic
GATAAATTAATGCAACAATG+GGG 0.695430 1_3:-5824952 Msa0099620:CDS
GTCGGATGCTAAGACTAACA+AGG 0.709457 1_3:-5824563 Msa0099620:CDS
CATAACTCTTCTAAACTCAG+TGG 0.721585 1_3:+5826080 None:intergenic
TAACTATGAGGATTATGACG+TGG 0.723957 1_3:-5825082 Msa0099620:CDS
TCGGCCGACTCAGAATGACG+TGG 0.772807 1_3:-5825832 Msa0099620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTATCTATCAT+AGG - chr1_3:5823544-5823563 Msa0099620:CDS 10.0%
!!! ATATTTGTTAAAACTTTCAA+GGG + chr1_3:5823982-5824001 None:intergenic 15.0%
!!! AATATTTGTTAAAACTTTCA+AGG + chr1_3:5823983-5824002 None:intergenic 15.0%
!!! TTGAAAGTTTTAACAAATAT+TGG - chr1_3:5823982-5824001 Msa0099620:CDS 15.0%
!! TCTGATTTATCTTTAATTAT+TGG - chr1_3:5825344-5825363 Msa0099620:CDS 15.0%
!! ATGAAGTGAATGAATTTATT+AGG - chr1_3:5823663-5823682 Msa0099620:CDS 20.0%
!! TGAAGTGAATGAATTTATTA+GGG - chr1_3:5823664-5823683 Msa0099620:CDS 20.0%
!!! TTGTTTTAGTTCTAACAATA+GGG - chr1_3:5824533-5824552 Msa0099620:intron 20.0%
!! TGATAAATTAATGCAACAAT+GGG - chr1_3:5825448-5825467 Msa0099620:CDS 20.0%
!! AAAAATAGGTAACGATATAT+TGG - chr1_3:5825709-5825728 Msa0099620:CDS 20.0%
!! TTATCTGTTAAATAACTACA+TGG + chr1_3:5826060-5826079 None:intergenic 20.0%
!!! ATTGAATCTCTTTTAATAGA+AGG - chr1_3:5826138-5826157 Msa0099620:CDS 20.0%
!!! TTGAATCTCTTTTAATAGAA+GGG - chr1_3:5826139-5826158 Msa0099620:CDS 20.0%
!! ATCACAGAATTATATAATGA+TGG - chr1_3:5826354-5826373 Msa0099620:CDS 20.0%
!! TCACAGAATTATATAATGAT+GGG - chr1_3:5826355-5826374 Msa0099620:CDS 20.0%
!!! TTTACAGTTTTACTTTTACA+TGG + chr1_3:5826964-5826983 None:intergenic 20.0%
!!! TTGACATGTATTGTATTTTA+TGG - chr1_3:5827012-5827031 Msa0099620:five_prime_UTR 20.0%
!! ATATTCCTTTTTCTCTTCTT+TGG - chr1_3:5823420-5823439 Msa0099620:three_prime_UTR 25.0%
!!! TAGAATGTTTTAAAATCGAG+AGG + chr1_3:5823510-5823529 None:intergenic 25.0%
! AACAAGATCAGTTAAAAACA+TGG + chr1_3:5823622-5823641 None:intergenic 25.0%
!! TTATTTTCAGACTCAAAGTT+TGG + chr1_3:5823727-5823746 None:intergenic 25.0%
!! ACTGAAACTGATTCAAATAT+TGG - chr1_3:5823760-5823779 Msa0099620:CDS 25.0%
!!! TAAGTTGAGATTTTGACAAA+CGG + chr1_3:5823882-5823901 None:intergenic 25.0%
! ACAAGAAATTGTCTTCAAAA+TGG - chr1_3:5823944-5823963 Msa0099620:CDS 25.0%
! AATTGTCTTCAAAATGGAAT+TGG - chr1_3:5823950-5823969 Msa0099620:CDS 25.0%
!! GCTAGATTAAATGTTAGTTT+CGG - chr1_3:5824405-5824424 Msa0099620:intron 25.0%
! AAGGATGATTTAATGAGATT+TGG - chr1_3:5824441-5824460 Msa0099620:intron 25.0%
!! AATCTGTCTTCAAGTTAATA+AGG + chr1_3:5824491-5824510 None:intergenic 25.0%
!!! CTTGTTTTAGTTCTAACAAT+AGG - chr1_3:5824532-5824551 Msa0099620:intron 25.0%
! TAATGTGGATGAGATTATTA+AGG - chr1_3:5824851-5824870 Msa0099620:CDS 25.0%
!! TTTAAGACAGTTTGAAAATG+AGG - chr1_3:5825223-5825242 Msa0099620:CDS 25.0%
!!! TTTTGATTTTAATGCGAATG+AGG - chr1_3:5825268-5825287 Msa0099620:CDS 25.0%
! CTGATAAATTAATGCAACAA+TGG - chr1_3:5825447-5825466 Msa0099620:CDS 25.0%
! GATAAATTAATGCAACAATG+GGG - chr1_3:5825449-5825468 Msa0099620:CDS 25.0%
! TCAAATGCTTGGAATAAAAA+AGG + chr1_3:5825958-5825977 None:intergenic 25.0%
! ACAGAAATAAATCAAATGCT+TGG + chr1_3:5825969-5825988 None:intergenic 25.0%
! TCAATCTTGTTAATAGTCAT+AGG + chr1_3:5826123-5826142 None:intergenic 25.0%
!!! AGAATTATATAATGATGGGT+TGG - chr1_3:5826359-5826378 Msa0099620:CDS 25.0%
!!! GAATTATATAATGATGGGTT+GGG - chr1_3:5826360-5826379 Msa0099620:CDS 25.0%
!! TTGATTTTGAATCTAGATTG+AGG + chr1_3:5826606-5826625 None:intergenic 25.0%
! TCAATCTAGATTCAAAATCA+AGG - chr1_3:5826605-5826624 Msa0099620:CDS 25.0%
! ATCTTGATTCAACAATTTGT+TGG + chr1_3:5826805-5826824 None:intergenic 25.0%
!! TATTGTATTTTATGGCCTAT+TGG - chr1_3:5827020-5827039 Msa0099620:five_prime_UTR 25.0%
AGAAGAAGAAAAAAAGTGGT+TGG + chr1_3:5823387-5823406 None:intergenic 30.0%
ATGGAGAAGAAGAAAAAAAG+TGG + chr1_3:5823391-5823410 None:intergenic 30.0%
AAAGGAATATTGTGTGGAAA+TGG + chr1_3:5823410-5823429 None:intergenic 30.0%
AGAGAAAAAGGAATATTGTG+TGG + chr1_3:5823416-5823435 None:intergenic 30.0%
ACTTACCAAAGAAGAGAAAA+AGG + chr1_3:5823428-5823447 None:intergenic 30.0%
ATTTGAAAGCAACAAAGGAA+AGG + chr1_3:5823475-5823494 None:intergenic 30.0%
!! GATTGATTTGAAAGCAACAA+AGG + chr1_3:5823480-5823499 None:intergenic 30.0%
TAAAAGACAGCAAAACAACA+TGG + chr1_3:5823588-5823607 None:intergenic 30.0%
GAGTCTGAAAATAAAGATGT+TGG - chr1_3:5823733-5823752 Msa0099620:CDS 30.0%
ATTAAGCAAAGCTCTTTACT+TGG - chr1_3:5823813-5823832 Msa0099620:CDS 30.0%
AACTTTGGTACATAGATGTT+CGG - chr1_3:5824167-5824186 Msa0099620:CDS 30.0%
TTGATTTATGCTTCTGTTGT+TGG - chr1_3:5824246-5824265 Msa0099620:CDS 30.0%
TGATTTATGCTTCTGTTGTT+GGG - chr1_3:5824247-5824266 Msa0099620:CDS 30.0%
ACTGAGTTTAGAAGAGTTAT+GGG - chr1_3:5824323-5824342 Msa0099620:CDS 30.0%
TTTCGGTTATCAAGTAATGA+AGG - chr1_3:5824422-5824441 Msa0099620:intron 30.0%
ACTAAAACAAGCAACATTGT+CGG + chr1_3:5824523-5824542 None:intergenic 30.0%
CTATATCAAAAGCTTGATGA+GGG - chr1_3:5824651-5824670 Msa0099620:CDS 30.0%
TATATCAAAAGCTTGATGAG+GGG - chr1_3:5824652-5824671 Msa0099620:CDS 30.0%
! AATTTTCCATCAGTGAACAA+GGG - chr1_3:5824748-5824767 Msa0099620:CDS 30.0%
CACATTAATGATTTCAACTG+TGG + chr1_3:5824838-5824857 None:intergenic 30.0%
CACAGTTGAAATCATTAATG+TGG - chr1_3:5824836-5824855 Msa0099620:CDS 30.0%
ATTCACATTCCTAGAACATA+CGG + chr1_3:5824925-5824944 None:intergenic 30.0%
TACACTTGAACATAACTATG+AGG - chr1_3:5825307-5825326 Msa0099620:CDS 30.0%
ATCAATCATTAATGCAGAGA+AGG - chr1_3:5825396-5825415 Msa0099620:CDS 30.0%
! CTCAAGATCTTCAAGAATTT+TGG + chr1_3:5825426-5825445 None:intergenic 30.0%
TCTTCATCGGATATTTCAAT+TGG + chr1_3:5825533-5825552 None:intergenic 30.0%
! ATTCTTAGCATTTCTGAAGA+GGG + chr1_3:5825642-5825661 None:intergenic 30.0%
! AATTCTTAGCATTTCTGAAG+AGG + chr1_3:5825643-5825662 None:intergenic 30.0%
!!! ATATCGTTACCTATTTTTGC+AGG + chr1_3:5825707-5825726 None:intergenic 30.0%
ATTGGATATACTTCTGTACA+TGG - chr1_3:5825727-5825746 Msa0099620:CDS 30.0%
ATCCATTAAGCATATTGTGT+CGG - chr1_3:5825820-5825839 Msa0099620:CDS 30.0%
TCCATGAATAATTCTGTTTC+AGG - chr1_3:5825998-5826017 Msa0099620:CDS 30.0%
TCCTGAAACAGAATTATTCA+TGG + chr1_3:5826002-5826021 None:intergenic 30.0%
TGAATAATTCTGTTTCAGGA+AGG - chr1_3:5826002-5826021 Msa0099620:CDS 30.0%
GAATAATTCTGTTTCAGGAA+GGG - chr1_3:5826003-5826022 Msa0099620:CDS 30.0%
AACAGATAAAGACAAATGCT+TGG - chr1_3:5826071-5826090 Msa0099620:CDS 30.0%
ACTGATAAGGTGAAAATAGA+TGG - chr1_3:5826390-5826409 Msa0099620:CDS 30.0%
! ACTGGTTCATAATTTCTTAG+AGG + chr1_3:5826477-5826496 None:intergenic 30.0%
CTAAGAAATTATGAACCAGT+TGG - chr1_3:5826477-5826496 Msa0099620:CDS 30.0%
AAGAGTTGACATACATGAAA+TGG - chr1_3:5826530-5826549 Msa0099620:CDS 30.0%
! ATTTTGAATCTAGATTGAGG+TGG + chr1_3:5826603-5826622 None:intergenic 30.0%
TGTTGAATCAAGATATCTTG+TGG - chr1_3:5826811-5826830 Msa0099620:five_prime_UTR 30.0%
CATGGATAGATTTCCTAAAT+TGG + chr1_3:5826946-5826965 None:intergenic 30.0%
AAGAAAAGATCATGTCCAAT+AGG + chr1_3:5827038-5827057 None:intergenic 30.0%
AGCAACAAAGGAAAGGAAAA+GGG + chr1_3:5823468-5823487 None:intergenic 35.0%
AAGCAACAAAGGAAAGGAAA+AGG + chr1_3:5823469-5823488 None:intergenic 35.0%
AACATGGTTATCATGTCATG+TGG + chr1_3:5823572-5823591 None:intergenic 35.0%
AGATGGTAGAAGGCTTAAAA+GGG + chr1_3:5823846-5823865 None:intergenic 35.0%
AGATCCTCACTTACAAATCT+CGG + chr1_3:5823913-5823932 None:intergenic 35.0%
TAAGTGAGGATCTGTTGATA+GGG - chr1_3:5823920-5823939 Msa0099620:CDS 35.0%
TCTTCATGTTCATTCGATTG+AGG - chr1_3:5824032-5824051 Msa0099620:CDS 35.0%
CTTCATGTTCATTCGATTGA+GGG - chr1_3:5824033-5824052 Msa0099620:CDS 35.0%
CTAACACCATTGAAACTCAA+AGG + chr1_3:5824063-5824082 None:intergenic 35.0%
! CTTTGAGTTTCAATGGTGTT+AGG - chr1_3:5824061-5824080 Msa0099620:CDS 35.0%
GTACATAGATGTTCGGTTTA+TGG - chr1_3:5824174-5824193 Msa0099620:CDS 35.0%
AGCATAAATCAAGAATCGCT+TGG + chr1_3:5824238-5824257 None:intergenic 35.0%
GATTTATGCTTCTGTTGTTG+GGG - chr1_3:5824248-5824267 Msa0099620:CDS 35.0%
CATAACTCTTCTAAACTCAG+TGG + chr1_3:5824324-5824343 None:intergenic 35.0%
CACTGAGTTTAGAAGAGTTA+TGG - chr1_3:5824322-5824341 Msa0099620:CDS 35.0%
CTGAGTTTAGAAGAGTTATG+GGG - chr1_3:5824324-5824343 Msa0099620:CDS 35.0%
TTATGGGGTGATAAAAGTTC+AGG - chr1_3:5824339-5824358 Msa0099620:CDS 35.0%
TATGGGGTGATAAAAGTTCA+GGG - chr1_3:5824340-5824359 Msa0099620:CDS 35.0%
GTGATAAAAGTTCAGGGAAA+TGG - chr1_3:5824346-5824365 Msa0099620:CDS 35.0%
! GGAAATGGAGTACAAGTTTT+AGG - chr1_3:5824361-5824380 Msa0099620:CDS 35.0%
!!! ATGGAGTACAAGTTTTAGGT+TGG - chr1_3:5824365-5824384 Msa0099620:CDS 35.0%
!!! AGTACAAGTTTTAGGTTGGT+TGG - chr1_3:5824369-5824388 Msa0099620:CDS 35.0%
!! ATGAGATTTGGTGCTAGTAA+TGG - chr1_3:5824453-5824472 Msa0099620:intron 35.0%
!! TGAGATTTGGTGCTAGTAAT+GGG - chr1_3:5824454-5824473 Msa0099620:intron 35.0%
TAAAACAAGCAACATTGTCG+GGG + chr1_3:5824521-5824540 None:intergenic 35.0%
CTAAAACAAGCAACATTGTC+GGG + chr1_3:5824522-5824541 None:intergenic 35.0%
AATGAAGCAGTGATGAATTC+TGG - chr1_3:5824600-5824619 Msa0099620:CDS 35.0%
! CCTCATCAAGCTTTTGATAT+AGG + chr1_3:5824653-5824672 None:intergenic 35.0%
CCTATATCAAAAGCTTGATG+AGG - chr1_3:5824650-5824669 Msa0099620:CDS 35.0%
GGAACTTCAATAATTCAGCA+CGG - chr1_3:5824673-5824692 Msa0099620:CDS 35.0%
GAACTTCAATAATTCAGCAC+GGG - chr1_3:5824674-5824693 Msa0099620:CDS 35.0%
! GAATTTTCCATCAGTGAACA+AGG - chr1_3:5824747-5824766 Msa0099620:CDS 35.0%
! ATTTTCCATCAGTGAACAAG+GGG - chr1_3:5824749-5824768 Msa0099620:CDS 35.0%
TCGTTCGAATTTGATCAAAC+AGG - chr1_3:5824792-5824811 Msa0099620:CDS 35.0%
!! ATGTTCTAGGAATGTGAATG+TGG - chr1_3:5824926-5824945 Msa0099620:CDS 35.0%
TAGGAATGTGAATGTGGATA+TGG - chr1_3:5824932-5824951 Msa0099620:CDS 35.0%
AACTGAATCCTCAATCTTCA+TGG + chr1_3:5825006-5825025 None:intergenic 35.0%
CACTTAAGTAGTGTTCTTCT+TGG + chr1_3:5825052-5825071 None:intergenic 35.0%
ACATAAAAAGCCACATAGCT+TGG - chr1_3:5825091-5825110 Msa0099620:CDS 35.0%
TGTCTTAAAAGCTTCTCTCT+AGG + chr1_3:5825212-5825231 None:intergenic 35.0%
!! TGATTTTAATGCGAATGAGG+AGG - chr1_3:5825271-5825290 Msa0099620:CDS 35.0%
!! GATTTTAATGCGAATGAGGA+GGG - chr1_3:5825272-5825291 Msa0099620:CDS 35.0%
TAACTATGAGGATTATGACG+TGG - chr1_3:5825319-5825338 Msa0099620:CDS 35.0%
!! ATCTTTAATTATTGGTGCCG+CGG - chr1_3:5825352-5825371 Msa0099620:CDS 35.0%
ATCATTAATGCAGAGAAGGA+AGG - chr1_3:5825400-5825419 Msa0099620:CDS 35.0%
TTGAAAATTCTCCAAGAACC+TGG - chr1_3:5825489-5825508 Msa0099620:CDS 35.0%
TAATATGGACGGTTCTTCAT+CGG + chr1_3:5825546-5825565 None:intergenic 35.0%
TTCACCAATCGAAGGTAATA+TGG + chr1_3:5825561-5825580 None:intergenic 35.0%
ATATTACCTTCGATTGGTGA+AGG - chr1_3:5825560-5825579 Msa0099620:CDS 35.0%
TATTACCTTCGATTGGTGAA+GGG - chr1_3:5825561-5825580 Msa0099620:CDS 35.0%
TCGTTTGTTCAAACTATGAG+CGG - chr1_3:5825590-5825609 Msa0099620:CDS 35.0%
TTTCCAGTGATATCTAGCAA+AGG + chr1_3:5825800-5825819 None:intergenic 35.0%
ATCCGACACAATATGCTTAA+TGG + chr1_3:5825825-5825844 None:intergenic 35.0%
CATCCAAGGAGAACATAATT+TGG - chr1_3:5826293-5826312 Msa0099620:CDS 35.0%
GTTCCAAATTATGTTCTCCT+TGG + chr1_3:5826299-5826318 None:intergenic 35.0%
!! TATAATGATGGGTTGGGAAA+TGG - chr1_3:5826366-5826385 Msa0099620:CDS 35.0%
ATCAAGGAGATTCATATTGC+AGG - chr1_3:5826621-5826640 Msa0099620:CDS 35.0%
TAGAGACTAATGCACACATT+AGG - chr1_3:5826859-5826878 Msa0099620:five_prime_UTR 35.0%
!!! CTTGCTGTTTTAGCCAATTT+AGG - chr1_3:5826930-5826949 Msa0099620:five_prime_UTR 35.0%
GAGTACAAATCTACAGAATC+GGG + chr1_3:5827076-5827095 None:intergenic 35.0%
GGAGTACAAATCTACAGAAT+CGG + chr1_3:5827077-5827096 None:intergenic 35.0%
AGGAAAAGGGTGATTGGAAA+TGG + chr1_3:5823455-5823474 None:intergenic 40.0%
AAGGAAAGGAAAAGGGTGAT+TGG + chr1_3:5823461-5823480 None:intergenic 40.0%
GATGGTAGAAGGCTTAAAAG+GGG + chr1_3:5823845-5823864 None:intergenic 40.0%
GAGATGGTAGAAGGCTTAAA+AGG + chr1_3:5823847-5823866 None:intergenic 40.0%
! TTTTCAGCAGAGATGGTAGA+AGG + chr1_3:5823856-5823875 None:intergenic 40.0%
AAACCCGAGATTTGTAAGTG+AGG - chr1_3:5823906-5823925 Msa0099620:CDS 40.0%
GATCCTCACTTACAAATCTC+GGG + chr1_3:5823912-5823931 None:intergenic 40.0%
GTAAGTGAGGATCTGTTGAT+AGG - chr1_3:5823919-5823938 Msa0099620:CDS 40.0%
! AAGTGAGGATCTGTTGATAG+GGG - chr1_3:5823921-5823940 Msa0099620:CDS 40.0%
! GGACTTCCTTTGAGTTTCAA+TGG - chr1_3:5824054-5824073 Msa0099620:CDS 40.0%
GTGTTAGGTTAAGTGTGCAT+TGG - chr1_3:5824076-5824095 Msa0099620:CDS 40.0%
GGTTAAGTGTGCATTGGAAA+AGG - chr1_3:5824082-5824101 Msa0099620:CDS 40.0%
TGCGGAGTTTGATGAAACTT+TGG - chr1_3:5824152-5824171 Msa0099620:CDS 40.0%
CATAGATGTTCGGTTTATGG+TGG - chr1_3:5824177-5824196 Msa0099620:CDS 40.0%
TGTTTCCCAATGTCATGTTC+AGG + chr1_3:5824276-5824295 None:intergenic 40.0%
ACATGACATTGGGAAACATC+AGG - chr1_3:5824278-5824297 Msa0099620:CDS 40.0%
!! AACATCAGGTTGATCTCACT+AGG - chr1_3:5824292-5824311 Msa0099620:CDS 40.0%
! AGGTTGGTTGGAAAAGCTTT+AGG - chr1_3:5824381-5824400 Msa0099620:CDS 40.0%
ATAGGGATGTCGTAAAGCTT+CGG - chr1_3:5824550-5824569 Msa0099620:CDS 40.0%
TAACGCAAGATGTGATTCAC+TGG - chr1_3:5824896-5824915 Msa0099620:CDS 40.0%
TCTACCATCCATGAAGATTG+AGG - chr1_3:5824995-5825014 Msa0099620:CDS 40.0%
GAATCCTCAATCTTCATGGA+TGG + chr1_3:5825002-5825021 None:intergenic 40.0%
ATGACATCATCCAAGCTATG+TGG + chr1_3:5825104-5825123 None:intergenic 40.0%
GAGTGATTTCTTAAAGACGC+TGG - chr1_3:5825136-5825155 Msa0099620:CDS 40.0%
TTTCTTAAAGACGCTGGCAT+TGG - chr1_3:5825142-5825161 Msa0099620:CDS 40.0%
AACAATGGGGTTTAGATGAG+AGG - chr1_3:5825462-5825481 Msa0099620:CDS 40.0%
ACAATGGGGTTTAGATGAGA+GGG - chr1_3:5825463-5825482 Msa0099620:CDS 40.0%
CCAATCGAAGGTAATATGGA+CGG + chr1_3:5825557-5825576 None:intergenic 40.0%
TTTGTTCAAACTATGAGCGG+AGG - chr1_3:5825593-5825612 Msa0099620:CDS 40.0%
! TTAGCATTTCTGAAGAGGGA+AGG + chr1_3:5825638-5825657 None:intergenic 40.0%
GATTAGAAGCCTGAATGATG+AGG + chr1_3:5825673-5825692 None:intergenic 40.0%
! ATTTTTGCAGGTAGAACGAC+TGG + chr1_3:5825695-5825714 None:intergenic 40.0%
GTCGTTCTACCTGCAAAAAT+AGG - chr1_3:5825695-5825714 Msa0099620:CDS 40.0%
ATGCCTTTGCTAGATATCAC+TGG - chr1_3:5825794-5825813 Msa0099620:CDS 40.0%
AAGACTAACAAGGAAGCTTC+TGG - chr1_3:5825848-5825867 Msa0099620:CDS 40.0%
GTCTAGCATGTCAAATGAAG+AGG - chr1_3:5826173-5826192 Msa0099620:CDS 40.0%
ATTTCTCCACGAATGCATGA+TGG + chr1_3:5826201-5826220 None:intergenic 40.0%
GAAATCAACGACGATCTTGA+TGG - chr1_3:5826216-5826235 Msa0099620:CDS 40.0%
TGTCAGTAACGTTGGATCAA+TGG - chr1_3:5826250-5826269 Msa0099620:CDS 40.0%
CAATGGTTGAGACTTGATTC+TGG - chr1_3:5826267-5826286 Msa0099620:CDS 40.0%
!!! TCCAATACTGGTTTTAGCTC+AGG - chr1_3:5826503-5826522 Msa0099620:CDS 40.0%
!!! GGTTACCACCATTTTTGGTA+AGG + chr1_3:5826650-5826669 None:intergenic 40.0%
!! TTGTCGGTTACCACCATTTT+TGG + chr1_3:5826655-5826674 None:intergenic 40.0%
GACAATATTACACATGCCAC+TGG + chr1_3:5826727-5826746 None:intergenic 40.0%
! CACTTTGGGAACATGATATC+AGG + chr1_3:5826888-5826907 None:intergenic 40.0%
GCTCTGACATTGTTCACTTT+GGG + chr1_3:5826902-5826921 None:intergenic 40.0%
TGCTCTGACATTGTTCACTT+TGG + chr1_3:5826903-5826922 None:intergenic 40.0%
CAAATCTACAGAATCGGGAA+GGG + chr1_3:5827071-5827090 None:intergenic 40.0%
ACAAATCTACAGAATCGGGA+AGG + chr1_3:5827072-5827091 None:intergenic 40.0%
TGAGATTTGAGAAGGTGGAG+TGG + chr1_3:5823364-5823383 None:intergenic 45.0%
! GTTGGTGAGATTTGAGAAGG+TGG + chr1_3:5823369-5823388 None:intergenic 45.0%
!! GTGGTTGGTGAGATTTGAGA+AGG + chr1_3:5823372-5823391 None:intergenic 45.0%
!!! ACGGTCTTTTTCAGCAGAGA+TGG + chr1_3:5823863-5823882 None:intergenic 45.0%
! TTTTGCAGACTTGCCCATCA+AGG - chr1_3:5824115-5824134 Msa0099620:CDS 45.0%
TTTGCAGACTTGCCCATCAA+GGG - chr1_3:5824116-5824135 Msa0099620:CDS 45.0%
AACCGTCCAATACCCTTGAT+GGG + chr1_3:5824131-5824150 None:intergenic 45.0%
GGGAACCTGAACATGACATT+GGG - chr1_3:5824268-5824287 Msa0099620:CDS 45.0%
AACACCACGTCATTCTGAGT+CGG + chr1_3:5824576-5824595 None:intergenic 45.0%
TTCTACCCCTTGTTCACTGA+TGG + chr1_3:5824757-5824776 None:intergenic 45.0%
AGGGGTAGAAACATCTGAAG+AGG - chr1_3:5824767-5824786 Msa0099620:CDS 45.0%
CACTGGATACCGTATGTTCT+AGG - chr1_3:5824913-5824932 Msa0099620:CDS 45.0%
GTCAAGAAACTCTGACGTCT+CGG + chr1_3:5825030-5825049 None:intergenic 45.0%
TCATTTCGCTCAAGCTGTGA+CGG - chr1_3:5825173-5825192 Msa0099620:CDS 45.0%
! AGCTTCTCTCTAGGAGACAT+GGG + chr1_3:5825203-5825222 None:intergenic 45.0%
! AAGCTTCTCTCTAGGAGACA+TGG + chr1_3:5825204-5825223 None:intergenic 45.0%
! GAAAATGAGGCTCTAGCTTC+AGG - chr1_3:5825236-5825255 Msa0099620:CDS 45.0%
TAATGCGAATGAGGAGGGAT+TGG - chr1_3:5825277-5825296 Msa0099620:CDS 45.0%
AATGCGAATGAGGAGGGATT+GGG - chr1_3:5825278-5825297 Msa0099620:CDS 45.0%
AATTCTCCAAGAACCTGGTC+TGG - chr1_3:5825494-5825513 Msa0099620:CDS 45.0%
CCGTCCATATTACCTTCGAT+TGG - chr1_3:5825554-5825573 Msa0099620:CDS 45.0%
GTCGGATGCTAAGACTAACA+AGG - chr1_3:5825838-5825857 Msa0099620:CDS 45.0%
CTAACAAGGAAGCTTCTGGA+AGG - chr1_3:5825852-5825871 Msa0099620:CDS 45.0%
TCTGTTTCAGGAAGGGATCA+TGG - chr1_3:5826010-5826029 Msa0099620:CDS 45.0%
! CGACGATCTTGATGGTTTGA+TGG - chr1_3:5826224-5826243 Msa0099620:CDS 45.0%
GATGGATCTGTCAGTAACGT+TGG - chr1_3:5826242-5826261 Msa0099620:CDS 45.0%
CTTGATTCTGGCATCATCCA+AGG - chr1_3:5826279-5826298 Msa0099620:CDS 45.0%
!! GTTGGGAAATGGCACTGATA+AGG - chr1_3:5826377-5826396 Msa0099620:CDS 45.0%
! TGGCAGAAAGCGCAGTTATT+TGG - chr1_3:5826410-5826429 Msa0099620:CDS 45.0%
! GGCAGAAAGCGCAGTTATTT+GGG - chr1_3:5826411-5826430 Msa0099620:CDS 45.0%
! TTTGGGAGAGCACATAACAG+TGG - chr1_3:5826428-5826447 Msa0099620:CDS 45.0%
TTCTTAGAGGATCTCTGTGC+TGG + chr1_3:5826464-5826483 None:intergenic 45.0%
ACCAGTTGGAGTTCCAATAC+TGG - chr1_3:5826491-5826510 Msa0099620:CDS 45.0%
! ACCAGTATTGGAACTCCAAC+TGG + chr1_3:5826495-5826514 None:intergenic 45.0%
GCCTGAGCTAAAACCAGTAT+TGG + chr1_3:5826507-5826526 None:intergenic 45.0%
GCAGGTGTCCTTACCAAAAA+TGG - chr1_3:5826639-5826658 Msa0099620:CDS 45.0%
GGTGTCCTTACCAAAAATGG+TGG - chr1_3:5826642-5826661 Msa0099620:CDS 45.0%
ATGGTGGTAACCGACAAGTT+TGG - chr1_3:5826658-5826677 Msa0099620:CDS 45.0%
TGGTGGTAACCGACAAGTTT+GGG - chr1_3:5826659-5826678 Msa0099620:CDS 45.0%
!! TAAAAAAAAAAAAAAAATAC+AGG + chr1_3:5823536-5823555 None:intergenic 5.0%
! GACTTGCCCATCAAGGGTAT+TGG - chr1_3:5824122-5824141 Msa0099620:CDS 50.0%
!! TGCCCATCAAGGGTATTGGA+CGG - chr1_3:5824126-5824145 Msa0099620:CDS 50.0%
GAACCGTCCAATACCCTTGA+TGG + chr1_3:5824132-5824151 None:intergenic 50.0%
!! AAGGGTATTGGACGGTTCTG+CGG - chr1_3:5824134-5824153 Msa0099620:CDS 50.0%
GGGGAACCTGAACATGACAT+TGG - chr1_3:5824267-5824286 Msa0099620:CDS 50.0%
!! GCAGTGATGAATTCTGGCTC+AGG - chr1_3:5824606-5824625 Msa0099620:CDS 50.0%
TAATTCAGCACGGGCAGACT+CGG - chr1_3:5824683-5824702 Msa0099620:CDS 50.0%
ATGCGAATGAGGAGGGATTG+GGG - chr1_3:5825279-5825298 Msa0099620:CDS 50.0%
!! AATTATTGGTGCCGCGGAAG+AGG - chr1_3:5825358-5825377 Msa0099620:CDS 50.0%
CCTTTCATACTCCTCTTCCG+CGG + chr1_3:5825372-5825391 None:intergenic 50.0%
! CGCGGAAGAGGAGTATGAAA+GGG - chr1_3:5825370-5825389 Msa0099620:CDS 50.0%
AATCCACCAGACCAGGTTCT+TGG + chr1_3:5825503-5825522 None:intergenic 50.0%
!! AGAACCTGGTCTGGTGGATT+TGG - chr1_3:5825503-5825522 Msa0099620:CDS 50.0%
ACTTCCAAATCCACCAGACC+AGG + chr1_3:5825510-5825529 None:intergenic 50.0%
! CTATGAGCGGAGGCTTTTTG+AGG - chr1_3:5825603-5825622 Msa0099620:CDS 50.0%
TCTGAAGAGGGAAGGACACA+TGG + chr1_3:5825630-5825649 None:intergenic 50.0%
TTGTGCGAGCCTCATCATTC+AGG - chr1_3:5825661-5825680 Msa0099620:CDS 50.0%
GGTGGTAACCGACAAGTTTG+GGG - chr1_3:5826660-5826679 Msa0099620:CDS 50.0%
ACGCAGTTCCCCAAACTTGT+CGG + chr1_3:5826671-5826690 None:intergenic 50.0%
ACTGCGTCTCAGCAACAATC+CGG - chr1_3:5826684-5826703 Msa0099620:CDS 50.0%
!! TTGCGTTGGTTGCTTTCCAG+TGG - chr1_3:5826708-5826727 Msa0099620:exon 50.0%
GCAGACTCGGAGTCATCTCA+AGG - chr1_3:5824696-5824715 Msa0099620:CDS 55.0%
! CCGCGGAAGAGGAGTATGAA+AGG - chr1_3:5825369-5825388 Msa0099620:CDS 55.0%
TCTCCAAGAACCTGGTCTGG+TGG - chr1_3:5825497-5825516 Msa0099620:CDS 55.0%
CCAAGCCCTTCACCAATCGA+AGG + chr1_3:5825569-5825588 None:intergenic 55.0%
CCTTCGATTGGTGAAGGGCT+TGG - chr1_3:5825566-5825585 Msa0099620:CDS 55.0%
! GAGGCACCATCATGCATTCG+TGG - chr1_3:5826192-5826211 Msa0099620:CDS 55.0%
CTGCGTCTCAGCAACAATCC+GGG - chr1_3:5826685-5826704 Msa0099620:CDS 55.0%
AGCAACAATCCGGGTTGCGT+TGG - chr1_3:5826694-5826713 Msa0099620:exon 55.0%
GGAAAGCAACCAACGCAACC+CGG + chr1_3:5826706-5826725 None:intergenic 55.0%
TATGGTGGTCGCGTCGTGTC+TGG - chr1_3:5824192-5824211 Msa0099620:CDS 60.0%
! TCGGCCGACTCAGAATGACG+TGG - chr1_3:5824569-5824588 Msa0099620:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 5823326 5827097 5823326 ID=Msa0099620;Name=Msa0099620
chr1_3 mRNA 5823326 5827097 5823326 ID=Msa0099620-mRNA-1;Parent=Msa0099620;Name=Msa0099620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=386|1|1|1|1|1|2|189|1016
chr1_3 exon 5823326 5824403 5823326 ID=Msa0099620-mRNA-1:exon:2250;Parent=Msa0099620-mRNA-1
chr1_3 exon 5824550 5827097 5824550 ID=Msa0099620-mRNA-1:exon:2249;Parent=Msa0099620-mRNA-1
chr1_3 five_prime_UTR 5826712 5827097 5826712 ID=Msa0099620-mRNA-1:five_prime_utr;Parent=Msa0099620-mRNA-1
chr1_3 CDS 5824550 5826711 5824550 ID=Msa0099620-mRNA-1:cds;Parent=Msa0099620-mRNA-1
chr1_3 CDS 5823515 5824403 5823515 ID=Msa0099620-mRNA-1:cds;Parent=Msa0099620-mRNA-1
chr1_3 three_prime_UTR 5823326 5823514 5823326 ID=Msa0099620-mRNA-1:three_prime_utr;Parent=Msa0099620-mRNA-1
Gene Sequence

>Msa0099620

ATGATGATGAAACCAAACTTTGAGTCTGAAAATAAAGATGTTGGTGAAACTGAAACTGATTCAAATATTGGTCATGAACAATTACTTCGTGACATTGAAGAATTAAGCAAAGCTCTTTACTTGGACAATACCCCTTTTAAGCCTTCTACCATCTCTGCTGAAAAAGACCGTTTGTCAAAATCTCAACTTAACTCAAACCCGAGATTTGTAAGTGAGGATCTGTTGATAGGGGACAAGAAATTGTCTTCAAAATGGAATTGGAAGAAGCCCTTGAAAGTTTTAACAAATATTGGTTCACAAAAGTTTGTTTGTTGCTTTAATCTTCATGTTCATTCGATTGAGGGACTTCCTTTGAGTTTCAATGGTGTTAGGTTAAGTGTGCATTGGAAAAGGAAAAACAGCATTTTGCAGACTTGCCCATCAAGGGTATTGGACGGTTCTGCGGAGTTTGATGAAACTTTGGTACATAGATGTTCGGTTTATGGTGGTCGCGTCGTGTCTGGTCGTTCTGTTAAGTATGAATCCAAGCGATTCTTGATTTATGCTTCTGTTGTTGGGGAACCTGAACATGACATTGGGAAACATCAGGTTGATCTCACTAGGCTTTTGCCACTGAGTTTAGAAGAGTTATGGGGTGATAAAAGTTCAGGGAAATGGAGTACAAGTTTTAGGTTGGTTGGAAAAGCTTTAGGAGCTAGATTAAATGTTAGTTTCGGTTATCAAGTAATGAAGGATGATTTAATGAGATTTGGTGCTAGTAATGGGAATATTGTTAACCTTATTAACTTGAAGACAGATTCAAGCATCCCCGACAATGTTGCTTGTTTTAGTTCTAACAATAGGGATGTCGTAAAGCTTCGGCCGACTCAGAATGACGTGGTGTTATCGAATGAAGCAGTGATGAATTCTGGCTCAGGTTTTTCTAAATCAATCACTTTCCTATATCAAAAGCTTGATGAGGGGAACTTCAATAATTCAGCACGGGCAGACTCGGAGTCATCTCAAGGATCAAATCTATATGTGTCTGATGACACTGAATTTTCCATCAGTGAACAAGGGGTAGAAACATCTGAAGAGGATTCGTTCGAATTTGATCAAACAGGAATTCAAATAATTGACATGTCCACAGTTGAAATCATTAATGTGGATGAGATTATTAAGGATGATGACACGTTTGCTGACAATAACGCAAGATGTGATTCACTGGATACCGTATGTTCTAGGAATGTGAATGTGGATATGGCAGATAACAGCAAACATCGATTCAGTATTTCATGTGTCGATCTACCATCCATGAAGATTGAGGATTCAGTTTCCGAGACGTCAGAGTTTCTTGACCAAGAAGAACACTACTTAAGTGTTAAATCAAATTATAAAGCACATAAAAAGCCACATAGCTTGGATGATGTCATCGACTCTGTCGCGAGTGATTTCTTAAAGACGCTGGCATTGGAGAGTGATTCATTTCGCTCAAGCTGTGACGGTGATCCCATGTCTCCTAGAGAGAAGCTTTTAAGACAGTTTGAAAATGAGGCTCTAGCTTCAGGCAACTTCGCTTTTGATTTTAATGCGAATGAGGAGGGATTGGGGCAATATACACTTGAACATAACTATGAGGATTATGACGTGGATTCTGATTTATCTTTAATTATTGGTGCCGCGGAAGAGGAGTATGAAAGGGAAAATCAATCATTAATGCAGAGAAGGAAGGCCAAAATTCTTGAAGATCTTGAGACTGATAAATTAATGCAACAATGGGGTTTAGATGAGAGGGACTTTGAAAATTCTCCAAGAACCTGGTCTGGTGGATTTGGAAGTCCAATTGAAATATCCGATGAAGAACCGTCCATATTACCTTCGATTGGTGAAGGGCTTGGTTCGTTTGTTCAAACTATGAGCGGAGGCTTTTTGAGGTCCATGTGTCCTTCCCTCTTCAGAAATGCTAAGAATTGTGCGAGCCTCATCATTCAGGCTTCTAATCCAGTCGTTCTACCTGCAAAAATAGGTAACGATATATTGGATATACTTCTGTACATGGCATCTGCTAGAGTTGAAGAGCTGTGCAATTATATCTCTAAATCGATGCCTTTGCTAGATATCACTGGAAAATCCATTAAGCATATTGTGTCGGATGCTAAGACTAACAAGGAAGCTTCTGGAAGGAAGGGATCATGGCAGCATGATTTGTTTGAAGAATTTCCATGTAGTTATTTAACAGATAAAGACAAATGCTTGGACTCTTTGTCTCTAGAAGCTATAGCTCCTATGACTATTAACAAGATTGAATCTCTTTTAATAGAAGGGTTGAGAATTCAGTCTAGCATGTCAAATGAAGAGGCACCATCATGCATTCGTGGAGAAATCAACGACGATCTTGATGGTTTGATGGATCTGTCAGTAACGTTGGATCAATGGTTGAGACTTGATTCTGGCATCATCCAAGGAGAACATAATTTGGAACAAATTTTGAAGATTCTTAAAGCACACAATTCTAAAATCACAGAATTATATAATGATGGGTTGGGAAATGGCACTGATAAGGTGAAAATAGATGGCAGAAAGCGCAGTTATTTGGGAGAGCACATAACAGTGGCTTTTATGATCCAGCACAGAGATCCTCTAAGAAATTATGAACCAGTTGGAGTTCCAATACTGGTTTTAGCTCAGGCAGAAAGAGTTGACATACATGAAATGGAGAAAGACTGCGACAATTTTGTTGAGAATGAAGATATAGATAAAGAACCACCTCAATCTAGATTCAAAATCAAGGAGATTCATATTGCAGGTGTCCTTACCAAAAATGGTGGTAACCGACAAGTTTGGGGAACTGCGTCTCAGCAACAATCCGGGTTGCGTTGGTTGCTTTCCAGTGGCATGTGTAATATTGTCAAACACTCTTCTTCAAAATCAAAGTCGATTGTTGTTAGATCATCTTCACTGTTTACCAACAAATTGTTGAATCAAGATATCTTGTGGAGCATCTCTTGTGTTGATAGTAACATAGAGACTAATGCACACATTAGGAATCCTGATATCATGTTCCCAAAGTGA
Protein sequence

>Msa0099620

MMMKPNFESENKDVGETETDSNIGHEQLLRDIEELSKALYLDNTPFKPSTISAEKDRLSKSQLNSNPRFVSEDLLIGDKKLSSKWNWKKPLKVLTNIGSQKFVCCFNLHVHSIEGLPLSFNGVRLSVHWKRKNSILQTCPSRVLDGSAEFDETLVHRCSVYGGRVVSGRSVKYESKRFLIYASVVGEPEHDIGKHQVDLTRLLPLSLEELWGDKSSGKWSTSFRLVGKALGARLNVSFGYQVMKDDLMRFGASNGNIVNLINLKTDSSIPDNVACFSSNNRDVVKLRPTQNDVVLSNEAVMNSGSGFSKSITFLYQKLDEGNFNNSARADSESSQGSNLYVSDDTEFSISEQGVETSEEDSFEFDQTGIQIIDMSTVEIINVDEIIKDDDTFADNNARCDSLDTVCSRNVNVDMADNSKHRFSISCVDLPSMKIEDSVSETSEFLDQEEHYLSVKSNYKAHKKPHSLDDVIDSVASDFLKTLALESDSFRSSCDGDPMSPREKLLRQFENEALASGNFAFDFNANEEGLGQYTLEHNYEDYDVDSDLSLIIGAAEEEYERENQSLMQRRKAKILEDLETDKLMQQWGLDERDFENSPRTWSGGFGSPIEISDEEPSILPSIGEGLGSFVQTMSGGFLRSMCPSLFRNAKNCASLIIQASNPVVLPAKIGNDILDILLYMASARVEELCNYISKSMPLLDITGKSIKHIVSDAKTNKEASGRKGSWQHDLFEEFPCSYLTDKDKCLDSLSLEAIAPMTINKIESLLIEGLRIQSSMSNEEAPSCIRGEINDDLDGLMDLSVTLDQWLRLDSGIIQGEHNLEQILKILKAHNSKITELYNDGLGNGTDKVKIDGRKRSYLGEHITVAFMIQHRDPLRNYEPVGVPILVLAQAERVDIHEMEKDCDNFVENEDIDKEPPQSRFKIKEIHIAGVLTKNGGNRQVWGTASQQQSGLRWLLSSGMCNIVKHSSSKSKSIVVRSSSLFTNKLLNQDILWSISCVDSNIETNAHIRNPDIMFPK*