AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300150 MtrunA17_Chr2g0324921 97.917 336 7 0 1 336 1 336 0.0 681
Msa0300150 MtrunA17_Chr4g0040501 68.362 354 83 5 1 335 1 344 2.06e-173 485
Msa0300150 MtrunA17_Chr2g0282741 66.476 349 93 4 1 335 1 339 1.14e-162 457
Msa0300150 MtrunA17_Chr4g0006391 65.330 349 99 6 1 336 1 340 1.55e-157 444
Msa0300150 MtrunA17_Chr2g0282761 56.447 349 121 5 1 328 1 339 9.06e-132 379
Msa0300150 MtrunA17_Chr3g0138471 54.783 345 129 5 1 336 1 327 2.44e-125 362
Msa0300150 MtrunA17_Chr3g0093111 43.750 336 161 3 3 335 1 311 3.80e-94 282
Msa0300150 MtrunA17_Chr5g0415751 58.621 145 60 0 190 334 16 160 8.36e-57 182
Msa0300150 MtrunA17_Chr3g0124801 44.693 179 98 1 157 334 113 291 3.51e-50 169
Msa0300150 MtrunA17_Chr7g0269841 34.513 339 191 8 5 334 27 343 8.48e-49 167
Msa0300150 MtrunA17_Chr2g0277721 43.333 180 101 1 156 334 80 259 2.52e-47 160
Msa0300150 MtrunA17_Chr8g0334811 31.313 396 158 11 5 334 15 362 5.00e-34 129
Msa0300150 MtrunA17_Chr2g0322181 40.690 145 84 1 192 334 214 358 9.48e-29 115
Msa0300150 MtrunA17_Chr2g0322181 57.746 71 25 1 5 75 14 79 1.56e-11 65.1
Msa0300150 MtrunA17_Chr4g0025401 40.690 145 84 1 192 334 212 356 6.97e-28 112
Msa0300150 MtrunA17_Chr3g0091351 50.000 96 48 0 220 315 2 97 3.43e-26 100
Msa0300150 MtrunA17_Chr5g0426951 27.933 358 210 12 4 332 83 421 3.03e-23 100
Msa0300150 MtrunA17_Chr6g0478651 35.714 182 78 5 2 183 66 208 1.50e-21 95.5
Msa0300150 MtrunA17_Chr3g0087741 28.242 347 209 9 4 327 89 418 9.61e-21 92.4
Msa0300150 MtrunA17_Chr7g0263971 56.522 69 29 1 4 72 369 436 3.88e-20 90.9
Msa0300150 MtrunA17_Chr4g0010781 25.843 356 215 9 1 327 83 418 1.40e-19 89.0
Msa0300150 MtrunA17_Chr5g0415731 56.000 75 26 1 4 71 5 79 1.50e-19 84.0
Msa0300150 MtrunA17_Chr2g0333221 42.609 115 44 3 4 118 70 162 7.62e-18 84.3
Msa0300150 MtrunA17_Chr3g0123861 54.795 73 31 1 3 75 81 151 1.19e-17 83.6
Msa0300150 MtrunA17_Chr7g0271891 53.030 66 28 1 5 67 17 82 3.38e-17 77.8
Msa0300150 MtrunA17_Chr4g0021141 50.746 67 32 1 4 70 22 87 2.09e-15 72.0
Msa0300150 MtrunA17_Chr8g0344721 49.275 69 30 1 4 67 10 78 4.49e-15 73.6
Msa0300150 MtrunA17_Chr5g0399201 45.946 74 38 1 4 75 12 85 1.94e-14 73.9
Msa0300150 MtrunA17_Chr5g0427221 51.562 64 30 1 4 67 48 110 2.99e-14 69.3
Msa0300150 MtrunA17_Chr2g0295821 36.641 131 59 4 4 125 104 219 8.20e-14 71.6
Msa0300150 MtrunA17_Chr1g0159491 46.377 69 36 1 3 71 5 72 1.59e-13 65.1
Msa0300150 MtrunA17_Chr1g0209551 47.887 71 36 1 4 74 16 85 3.05e-13 70.1
Msa0300150 MtrunA17_Chr7g0242951 47.143 70 35 1 3 72 26 93 3.50e-13 70.5
Msa0300150 MtrunA17_Chr4g0044331 50.000 60 27 1 11 67 19 78 6.14e-13 65.9
Msa0300150 MtrunA17_Chr5g0414471 46.575 73 38 1 4 76 25 96 1.24e-12 68.2
Msa0300150 MtrunA17_Chr1g0173931 47.692 65 33 1 6 70 40 103 2.97e-12 66.2
Msa0300150 MtrunA17_Chr6g0460461 37.895 95 41 2 3 93 1 81 3.16e-12 65.1
Msa0300150 MtrunA17_Chr6g0460411 37.895 95 41 2 3 93 1 81 3.30e-12 65.1
Msa0300150 MtrunA17_Chr7g0258061 44.595 74 37 2 6 76 24 96 1.00e-11 64.7
Msa0300150 MtrunA17_Chr6g0460381 42.029 69 36 1 3 67 2 70 3.05e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300150 AT2G20560.1 71.637 342 83 3 1 334 1 336 1.20e-165 465
Msa0300150 AT4G28480.1 69.801 351 85 6 1 334 1 347 7.92e-157 443
Msa0300150 AT3G08910.1 65.476 336 102 4 1 336 1 322 1.01e-154 437
Msa0300150 AT5G01390.1 59.710 345 118 5 1 335 1 334 3.18e-142 405
Msa0300150 AT5G25530.2 55.000 360 117 6 1 336 1 339 7.93e-135 387
Msa0300150 AT5G25530.1 53.804 368 117 7 1 336 1 347 6.34e-132 380
Msa0300150 AT1G10350.1 52.571 350 150 5 1 336 1 348 1.82e-124 361
Msa0300150 AT3G47940.1 54.000 350 145 4 1 336 1 348 1.64e-119 348
Msa0300150 AT1G59725.1 55.786 337 141 4 1 336 1 330 3.00e-119 347
Msa0300150 AT5G01390.2 53.550 338 92 5 1 335 1 276 3.25e-116 337
Msa0300150 AT4G28480.2 55.425 341 93 10 1 334 1 289 4.85e-99 294
Msa0300150 AT2G20550.1 58.242 273 85 4 84 334 18 283 6.03e-92 276
Msa0300150 AT2G20550.2 58.242 273 85 4 84 334 18 283 6.03e-92 276
Msa0300150 AT5G01390.3 45.562 338 80 6 1 335 1 237 3.72e-86 259
Msa0300150 AT5G01390.4 54.369 206 73 4 1 196 1 195 9.85e-64 202
Msa0300150 AT1G44160.1 46.629 178 95 0 157 334 176 353 9.27e-50 170
Msa0300150 AT1G11040.1 40.331 181 108 0 154 334 252 432 1.19e-45 161
Msa0300150 AT3G62600.1 34.393 346 192 9 2 335 24 346 3.11e-44 155
Msa0300150 AT5G22060.1 32.800 375 177 10 5 334 15 359 4.72e-39 143
Msa0300150 AT3G44110.1 33.428 353 203 8 5 334 15 358 1.73e-38 142
Msa0300150 AT3G44110.2 32.515 326 188 8 5 307 15 331 2.20e-30 118
Msa0300150 AT5G48030.1 26.816 358 209 13 4 327 94 432 4.50e-22 97.1
Msa0300150 AT1G80030.4 43.651 126 47 5 4 129 75 176 4.18e-19 88.6
Msa0300150 AT1G80030.2 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0300150 AT1G80030.3 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0300150 AT1G80030.1 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0300150 AT2G22360.1 55.405 74 31 1 3 76 85 156 6.88e-18 84.7
Msa0300150 AT4G39960.1 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0300150 AT4G39960.2 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0300150 AT5G03160.1 53.623 69 31 1 4 72 370 437 2.74e-17 82.8
Msa0300150 AT1G56300.2 53.030 66 28 1 5 67 14 79 8.20e-17 75.9
Msa0300150 AT1G56300.1 53.030 66 28 1 5 67 14 79 9.66e-17 77.0
Msa0300150 AT2G35720.1 41.975 81 45 1 4 82 15 95 3.61e-14 73.6
Msa0300150 AT5G16650.2 50.746 67 32 1 4 70 11 76 6.02e-14 69.3
Msa0300150 AT5G16650.1 51.562 64 30 1 4 67 11 73 7.78e-14 68.2
Msa0300150 AT2G41000.2 46.377 69 33 1 3 67 2 70 3.95e-13 68.2
Msa0300150 AT3G17830.1 38.849 139 51 3 2 140 61 165 5.12e-13 70.1
Msa0300150 AT1G68370.1 47.887 71 36 1 4 74 17 86 8.74e-13 69.3
Msa0300150 AT2G41000.1 46.377 69 33 1 3 67 2 70 9.48e-13 66.2
Msa0300150 AT3G08970.1 48.000 75 35 2 3 77 26 96 1.04e-12 69.3
Msa0300150 AT3G08970.2 48.000 75 35 2 3 77 26 96 1.04e-12 69.3
Msa0300150 AT5G05750.1 34.266 143 73 4 4 146 114 235 1.07e-12 68.2
Msa0300150 AT5G06910.1 50.769 65 31 1 6 70 31 94 1.08e-12 67.8
Msa0300150 AT1G71000.2 46.970 66 32 1 5 67 9 74 3.24e-12 64.3
Msa0300150 AT2G21510.3 47.222 72 37 1 4 75 6 76 3.60e-12 67.0
Msa0300150 AT2G21510.1 47.222 72 37 1 4 75 6 76 3.60e-12 67.0
Msa0300150 AT1G59980.2 42.029 69 39 1 6 74 25 92 3.75e-12 67.0
Msa0300150 AT2G21510.2 47.222 72 37 1 4 75 6 76 4.01e-12 67.0
Msa0300150 AT2G21510.4 47.222 72 37 1 4 75 6 76 4.01e-12 67.0
Msa0300150 AT1G71000.1 46.970 66 32 1 5 67 9 74 5.18e-12 63.9
Msa0300150 AT2G21510.5 47.222 72 37 1 4 75 6 76 5.52e-12 66.2
Msa0300150 AT1G59980.1 42.029 69 39 1 6 74 25 92 6.19e-12 66.6
Msa0300150 AT3G14200.1 46.875 64 32 1 6 67 14 77 7.52e-12 64.7
Msa0300150 AT1G72416.3 45.455 66 34 1 4 67 5 70 1.43e-11 63.5
Msa0300150 AT5G49060.2 33.913 115 67 2 4 118 99 204 1.59e-11 65.1
Msa0300150 AT5G49060.1 33.913 115 67 2 4 118 99 204 1.59e-11 65.1
Msa0300150 AT1G72416.4 45.455 66 34 1 4 67 5 70 1.88e-11 62.8
Msa0300150 AT1G72416.1 45.455 66 34 1 4 67 5 70 2.05e-11 62.8
Msa0300150 AT5G37750.1 39.130 92 54 1 4 95 70 159 2.16e-11 63.5
Msa0300150 AT1G72416.2 45.455 66 34 1 4 67 5 70 2.63e-11 62.4
Msa0300150 AT2G33735.1 43.077 65 36 1 4 68 22 85 2.97e-11 60.5
Msa0300150 AT2G33735.2 43.077 65 36 1 4 68 22 85 2.97e-11 60.5
Msa0300150 AT1G24120.1 41.892 74 42 1 4 77 20 92 3.38e-11 64.3
Msa0300150 AT4G19570.1 51.515 66 30 1 3 68 65 128 4.05e-11 64.3

Find 124 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCTTTGTTGGATCCTT+TGG 0.129787 2_3:-55518191 None:intergenic
GTTAATGTTACATTAAAATT+TGG 0.135986 2_3:+55518517 Msa0300150:three_prime_UTR
CAGGAGGCAATTGGGAAATT+TGG 0.163872 2_3:+55518378 Msa0300150:three_prime_UTR
TTCTGAGATTTGCTTAAATT+TGG 0.199073 2_3:-55515345 None:intergenic
CTCAAGTTTCCCTTCTTTGT+TGG 0.262625 2_3:-55518200 None:intergenic
CACAAATATATGAATGCTTA+TGG 0.271161 2_3:-55518607 None:intergenic
ATATCAGCAGGTATGGTATT+TGG 0.272102 2_3:-55517960 None:intergenic
CCTTCTTTGTTGGATCCTTT+GGG 0.278902 2_3:-55518190 None:intergenic
ATTGGGAAATTTGGTTGTTA+TGG 0.283905 2_3:+55518387 Msa0300150:three_prime_UTR
AGAGCTGTTTATGATCAATA+TGG 0.297643 2_3:+55515530 Msa0300150:CDS
TCTCTTGAGGATATATATAA+AGG 0.310218 2_3:+55515845 Msa0300150:CDS
ACCAACATTATTCAACTTCA+TGG 0.313695 2_3:+55518553 Msa0300150:three_prime_UTR
TGGATGTTCATTTCCCTTCT+CGG 0.321746 2_3:-55517940 None:intergenic
TTTCTTCAAGTCATCGTCTT+TGG 0.326757 2_3:-55515255 None:intergenic
ATTGCCTCCTGTCAAGATTA+TGG 0.339096 2_3:-55518366 None:intergenic
GGATAAAGTAAGATATTAAT+TGG 0.350954 2_3:+55518296 Msa0300150:exon
TTGTTATTAGGGTTCTTATC+AGG 0.352145 2_3:-55515308 None:intergenic
GGGAGTGGCATTCCTTCTCT+TGG 0.354044 2_3:-55518170 None:intergenic
AGAGGTAGCAATGGCATGTT+TGG 0.355428 2_3:+55515734 Msa0300150:CDS
GGTGATCTTCATCTTCTTAG+TGG 0.355739 2_3:-55515870 None:intergenic
GGATGTTCATTTCCCTTCTC+GGG 0.357845 2_3:-55517939 None:intergenic
TTAATGTTACATTAAAATTT+GGG 0.367373 2_3:+55518518 Msa0300150:three_prime_UTR
TAATCTTGACAGGAGGCAAT+TGG 0.388489 2_3:+55518369 Msa0300150:three_prime_UTR
CTACCAGAGGCATGAGAATC+AGG 0.390225 2_3:-55515587 None:intergenic
GCCGCAAAATCGCAGAAAAT+CGG 0.395524 2_3:+55515133 Msa0300150:five_prime_UTR
TTTGTCCCCTTCTTCCATCC+AGG 0.403890 2_3:-55517906 None:intergenic
GGAATGGGAGGGATGGGAAG+AGG 0.407566 2_3:+55515716 Msa0300150:CDS
CGAAATCCCTTTGGTGGAAT+GGG 0.411716 2_3:+55515701 Msa0300150:CDS
CTTATCAGGATGCCACTTCA+TGG 0.411951 2_3:-55515294 None:intergenic
CATAGCAACTTGTTCTCAAT+AGG 0.424637 2_3:-55515821 None:intergenic
CATTATTCAACTTCATGGTA+TGG 0.433834 2_3:+55518558 Msa0300150:three_prime_UTR
GAGCTGTTTATGATCAATAT+GGG 0.439767 2_3:+55515531 Msa0300150:CDS
GTGAATCTCACTACACTTGA+TGG 0.443477 2_3:+55518089 Msa0300150:CDS
TTTGGGGATCGAAATCCCTT+TGG 0.447674 2_3:+55515692 Msa0300150:CDS
TATGATCAATATGGGGAAGA+TGG 0.451224 2_3:+55515539 Msa0300150:CDS
GATTCTCATGCCTCTGGTAG+TGG 0.458632 2_3:+55515590 Msa0300150:CDS
TCGAAATCCCTTTGGTGGAA+TGG 0.459463 2_3:+55515700 Msa0300150:CDS
ATTATTCAACTTCATGGTAT+GGG 0.463867 2_3:+55518559 Msa0300150:three_prime_UTR
TTTGTTGGATCCTTTGGGAG+TGG 0.464104 2_3:-55518185 None:intergenic
AACTGATACGGAAAGTCATC+AGG 0.465543 2_3:-55515632 None:intergenic
GTTAGACAATGAGTTACATT+GGG 0.467007 2_3:+55518477 Msa0300150:three_prime_UTR
CTCTTGAGGATATATATAAA+GGG 0.467907 2_3:+55515846 Msa0300150:CDS
GATCCGAAGTGAGCCGAGTT+GGG 0.468185 2_3:-55518244 None:intergenic
TCTCTTGCAGAAGCCTTAGC+TGG 0.474179 2_3:+55518059 Msa0300150:CDS
CCCAAAGGATCCAACAAAGA+AGG 0.480916 2_3:+55518190 Msa0300150:CDS
GGGGATCGAAATCCCTTTGG+TGG 0.482474 2_3:+55515695 Msa0300150:CDS
AATCTTGACAGGAGGCAATT+GGG 0.488582 2_3:+55518370 Msa0300150:three_prime_UTR
TAGATTACCATAATCTTGAC+AGG 0.489863 2_3:+55518359 Msa0300150:three_prime_UTR
GTCAAACCTGGATGGAAGAA+GGG 0.491815 2_3:+55517900 Msa0300150:CDS
GAAAATGATATCAGCAGGTA+TGG 0.491993 2_3:-55517967 None:intergenic
TATGGTGATGCTGGAGGAAG+TGG 0.494603 2_3:+55515761 Msa0300150:CDS
TGTTGTTCATCCTGAGTATG+AGG 0.496005 2_3:+55518139 Msa0300150:CDS
TGATCCGAAGTGAGCCGAGT+TGG 0.497615 2_3:-55518245 None:intergenic
ACTGTGATCAAGAATTTCTC+TGG 0.501571 2_3:-55515891 None:intergenic
CTTTGGTGGAATGGGAGGGA+TGG 0.502219 2_3:+55515709 Msa0300150:CDS
TTTGGTGGAATGGGAGGGAT+GGG 0.503447 2_3:+55515710 Msa0300150:CDS
TGTCAAACCTGGATGGAAGA+AGG 0.504580 2_3:+55517899 Msa0300150:CDS
TTTGGATCCTATGGTGATGC+TGG 0.505971 2_3:+55515752 Msa0300150:CDS
GGTTAGACAATGAGTTACAT+TGG 0.506915 2_3:+55518476 Msa0300150:three_prime_UTR
ATCCCTCCCATTCCACCAAA+GGG 0.515203 2_3:-55515707 None:intergenic
GTCCGATGATTGAACTGATA+CGG 0.527889 2_3:-55515644 None:intergenic
ATGATCAATATGGGGAAGAT+GGG 0.529413 2_3:+55515540 Msa0300150:CDS
CCACCTGATTCTCATGCCTC+TGG 0.530969 2_3:+55515584 Msa0300150:CDS
AATGGCATGTTTGGATCCTA+TGG 0.531436 2_3:+55515743 Msa0300150:CDS
ACAAGCTTCTGCAGGTTGAT+CGG 0.533594 2_3:+55515228 Msa0300150:CDS
ACCATGAAGTTGAATAATGT+TGG 0.535476 2_3:-55518554 None:intergenic
AGAAGGGCAACAACCTGCCA+TGG 0.538305 2_3:+55515193 Msa0300150:exon
ATGATGAAAATGATATCAGC+AGG 0.541292 2_3:-55517972 None:intergenic
GCATGAGAATCAGGTGGTGG+TGG 0.542892 2_3:-55515578 None:intergenic
TCGGCTCACTTCGGATCAGA+AGG 0.545186 2_3:+55518250 Msa0300150:CDS
AAAGATCACTTTCCCCGAGA+AGG 0.545291 2_3:+55517926 Msa0300150:CDS
CATCCCTCCCATTCCACCAA+AGG 0.550801 2_3:-55515708 None:intergenic
ATATGGGGAAGATGGGCTGA+AGG 0.551422 2_3:+55515547 Msa0300150:CDS
CTCACTTCGGATCAGAAGGC+AGG 0.551807 2_3:+55518254 Msa0300150:CDS
GGAATGAAGAAAGTCTTGGC+TGG 0.552067 2_3:+55518275 Msa0300150:CDS
CTGTCGAAGAGAAAGAGAGA+AGG 0.555619 2_3:+55515176 Msa0300150:five_prime_UTR
TTCCGTATCAGTTCAATCAT+CGG 0.556341 2_3:+55515642 Msa0300150:CDS
GGAACAACCTCCTCATACTC+AGG 0.557628 2_3:-55518149 None:intergenic
GTGCAGCAAAGTCACATCGC+TGG 0.559149 2_3:+55517857 Msa0300150:intron
GCATCAAGGTCCTCCTCGTA+AGG 0.559593 2_3:+55515793 Msa0300150:CDS
ACAGAAAACTCGCCATGAAG+TGG 0.563345 2_3:+55515282 Msa0300150:CDS
AAGATCACTTTCCCCGAGAA+GGG 0.565073 2_3:+55517927 Msa0300150:CDS
CACAATGTGTTCACTCGTGA+AGG 0.567306 2_3:+55518008 Msa0300150:CDS
CCAAAGGATCCAACAAAGAA+GGG 0.568075 2_3:+55518191 Msa0300150:CDS
AGCAACTTGTTCTCAATAGG+AGG 0.568821 2_3:-55515818 None:intergenic
TGTCGAAGAGAAAGAGAGAA+GGG 0.570583 2_3:+55515177 Msa0300150:five_prime_UTR
TAGTAGTCAACACCCATGGC+AGG 0.572331 2_3:-55515206 None:intergenic
AATCCCTTTGGTGGAATGGG+AGG 0.575318 2_3:+55515704 Msa0300150:CDS
CAATTAGTGTCAAACCTGGA+TGG 0.581052 2_3:+55517892 Msa0300150:CDS
GGCAGGAATGAAGAAAGTCT+TGG 0.581140 2_3:+55518271 Msa0300150:CDS
ATTCCCAACTCGGCTCACTT+CGG 0.581525 2_3:+55518241 Msa0300150:CDS
GAGAAATTCTTGATCACAGT+GGG 0.581668 2_3:+55515894 Msa0300150:CDS
TCACGAGTGAACACATTGTG+TGG 0.583017 2_3:-55518005 None:intergenic
ACTTCCTCCAGCATCACCAT+AGG 0.584524 2_3:-55515759 None:intergenic
TATGGGGAAGATGGGCTGAA+GGG 0.585281 2_3:+55515548 Msa0300150:CDS
TGACTACTACAAGCTTCTGC+AGG 0.587066 2_3:+55515220 Msa0300150:CDS
TTGACAATTAGTGTCAAACC+TGG 0.594845 2_3:+55517888 Msa0300150:CDS
AGTGGAGTCAATGTGCATCA+AGG 0.595190 2_3:+55515779 Msa0300150:CDS
TCTCATGCCTCTGGTAGTGG+TGG 0.595916 2_3:+55515593 Msa0300150:CDS
ATCCCTTTGGTGGAATGGGA+GGG 0.596841 2_3:+55515705 Msa0300150:CDS
TTATTCAACTTCATGGTATG+GGG 0.599396 2_3:+55518560 Msa0300150:three_prime_UTR
GAAGGGCAACAACCTGCCAT+GGG 0.605250 2_3:+55515194 Msa0300150:exon
ATTCACTGTACAACCAGCTA+AGG 0.605987 2_3:-55518072 None:intergenic
GCTTAGGTACAATACAATCG+TGG 0.609230 2_3:+55515053 None:intergenic
GAGGAGGTTGTTCCAAGAGA+AGG 0.609654 2_3:+55518158 Msa0300150:CDS
GAGGCATGAGAATCAGGTGG+TGG 0.609693 2_3:-55515581 None:intergenic
AGAAGGAATGCCACTCCCAA+AGG 0.611731 2_3:+55518175 Msa0300150:CDS
GATGTTCATTTCCCTTCTCG+GGG 0.615186 2_3:-55517938 None:intergenic
CTTAGGTACAATACAATCGT+GGG 0.615629 2_3:+55515054 None:intergenic
TCAAACCTGGATGGAAGAAG+GGG 0.618407 2_3:+55517901 Msa0300150:CDS
TCAATAGGAGGCGCCTTACG+AGG 0.622058 2_3:-55515806 None:intergenic
TCAACGTCAAATTCCCAACT+CGG 0.622716 2_3:+55518231 Msa0300150:CDS
CAAGTTGCTATGTTCTCTTG+AGG 0.636918 2_3:+55515832 Msa0300150:CDS
GGGATGGGAAGAGGTAGCAA+TGG 0.641843 2_3:+55515725 Msa0300150:CDS
CAACAAAGAAGGGAAACTTG+AGG 0.650514 2_3:+55518201 Msa0300150:CDS
GGATCCTATGGTGATGCTGG+AGG 0.665018 2_3:+55515755 Msa0300150:CDS
ATTACCATAATCTTGACAGG+AGG 0.665326 2_3:+55518362 Msa0300150:three_prime_UTR
CTTGTAGTAGTCAACACCCA+TGG 0.668721 2_3:-55515210 None:intergenic
ATAGGAGGCGCCTTACGAGG+AGG 0.673195 2_3:-55515803 None:intergenic
AGCTGTTTATGATCAATATG+GGG 0.674859 2_3:+55515532 Msa0300150:CDS
TCTACTACCACCACTACCAG+AGG 0.706463 2_3:-55515600 None:intergenic
CCAGAGGCATGAGAATCAGG+TGG 0.707871 2_3:-55515584 None:intergenic
TGTTCATCCTGAGTATGAGG+AGG 0.716066 2_3:+55518142 Msa0300150:CDS
AGAGAAATTCTTGATCACAG+TGG 0.741173 2_3:+55515893 Msa0300150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAACTTTTTTTTTTTTTT+TGG + chr2_3:55516782-55516801 Msa0300150:intron 10.0%
!!! AGAACTTTTTTTTTTTTTTT+GGG + chr2_3:55516783-55516802 Msa0300150:intron 10.0%
!!! ATTTAAAATTTTGAAAAAGT+GGG - chr2_3:55516945-55516964 None:intergenic 10.0%
!!! AATTTAAAATTTTGAAAAAG+TGG - chr2_3:55516946-55516965 None:intergenic 10.0%
!! TTAATGTTACATTAAAATTT+GGG + chr2_3:55518518-55518537 Msa0300150:three_prime_UTR 10.0%
!!! ATGATGTAAGTTTTTTTTAA+AGG + chr2_3:55515372-55515391 Msa0300150:intron 15.0%
!!! TGATGTAAGTTTTTTTTAAA+GGG + chr2_3:55515373-55515392 Msa0300150:intron 15.0%
!!! CTTAAATTTTCAATTTTTAG+TGG + chr2_3:55515399-55515418 Msa0300150:intron 15.0%
!! TATAAATATCTAATAACTGA+TGG - chr2_3:55515971-55515990 None:intergenic 15.0%
!! TAAACAATTACTATAATTCT+AGG - chr2_3:55516629-55516648 None:intergenic 15.0%
!! TATAAATCAACATTATGATA+TGG - chr2_3:55517195-55517214 None:intergenic 15.0%
!!! TAGTATAGTAATCTATTTTT+TGG + chr2_3:55518426-55518445 Msa0300150:three_prime_UTR 15.0%
!!! TATTTTTTGGAATGATATTT+TGG + chr2_3:55518439-55518458 Msa0300150:three_prime_UTR 15.0%
!! GTTAATGTTACATTAAAATT+TGG + chr2_3:55518517-55518536 Msa0300150:three_prime_UTR 15.0%
!!! GGAATTTGTTTTTTGTTATT+AGG + chr2_3:55515487-55515506 Msa0300150:intron 20.0%
!!! ATATTTTTGCTGAGTTTTTT+GGG + chr2_3:55515675-55515694 Msa0300150:CDS 20.0%
!! TCATAATATTAGATAGATAG+TGG + chr2_3:55516179-55516198 Msa0300150:intron 20.0%
!! CATAATATTAGATAGATAGT+GGG + chr2_3:55516180-55516199 Msa0300150:intron 20.0%
!! ATAATATTAGATAGATAGTG+GGG + chr2_3:55516181-55516200 Msa0300150:intron 20.0%
!! GTTCTATTAGAAAAAATTCA+AGG - chr2_3:55516768-55516787 None:intergenic 20.0%
!!! CTTTTTTTTTTTTTTTGGGT+AGG + chr2_3:55516787-55516806 Msa0300150:intron 20.0%
!! TTGATTTATAGTAGTATGTT+TGG + chr2_3:55517205-55517224 Msa0300150:intron 20.0%
!! TGATTTATAGTAGTATGTTT+GGG + chr2_3:55517206-55517225 Msa0300150:intron 20.0%
!! TTTAATGTTGCAAATTACAA+TGG + chr2_3:55517450-55517469 Msa0300150:intron 20.0%
!!! TTGATGTTTTTCTAGTATAA+TGG + chr2_3:55517675-55517694 Msa0300150:intron 20.0%
!! ATTCTAGATGTTTAAATTAG+AGG - chr2_3:55517728-55517747 None:intergenic 20.0%
!! AAAAAACAAAGTCATCAATA+GGG - chr2_3:55517823-55517842 None:intergenic 20.0%
!! AAAAAAACAAAGTCATCAAT+AGG - chr2_3:55517824-55517843 None:intergenic 20.0%
!! GGATAAAGTAAGATATTAAT+TGG + chr2_3:55518296-55518315 Msa0300150:exon 20.0%
!!! TGATATTTTGGTCTATTTTA+TGG + chr2_3:55518451-55518470 Msa0300150:three_prime_UTR 20.0%
!!! GTTCAAGAAATTTTAACGTT+GGG - chr2_3:55515094-55515113 None:intergenic 25.0%
!!! AGTTCAAGAAATTTTAACGT+TGG - chr2_3:55515095-55515114 None:intergenic 25.0%
!!! CAGCTTCTTTTTTGTTATTA+GGG - chr2_3:55515322-55515341 None:intergenic 25.0%
!!! TCAGCTTCTTTTTTGTTATT+AGG - chr2_3:55515323-55515342 None:intergenic 25.0%
!!! TTCTGAGATTTGCTTAAATT+TGG - chr2_3:55515348-55515367 None:intergenic 25.0%
!!! AAAGTTTTGATTTTTGCATC+TGG + chr2_3:55515444-55515463 Msa0300150:intron 25.0%
!!! AAGTTTTGATTTTTGCATCT+GGG + chr2_3:55515445-55515464 Msa0300150:intron 25.0%
!!! GATATTTTTGCTGAGTTTTT+TGG + chr2_3:55515674-55515693 Msa0300150:CDS 25.0%
!!! TATTTTTGCTGAGTTTTTTG+GGG + chr2_3:55515676-55515695 Msa0300150:CDS 25.0%
! TCTCTTGAGGATATATATAA+AGG + chr2_3:55515845-55515864 Msa0300150:CDS 25.0%
! CTCTTGAGGATATATATAAA+GGG + chr2_3:55515846-55515865 Msa0300150:CDS 25.0%
!! TAATATTAGATAGATAGTGG+GGG + chr2_3:55516182-55516201 Msa0300150:intron 25.0%
! TAATGAGTTGTATCGAAATA+TGG + chr2_3:55516388-55516407 Msa0300150:intron 25.0%
! TTGTATCGAAATATGGTTTA+TGG + chr2_3:55516395-55516414 Msa0300150:intron 25.0%
! GAACCATCAATACTTAAAAT+TGG - chr2_3:55516703-55516722 None:intergenic 25.0%
! CGAGTTATATTGGTATAAAT+TGG + chr2_3:55517055-55517074 Msa0300150:intron 25.0%
! GATTTATAGTAGTATGTTTG+GGG + chr2_3:55517207-55517226 Msa0300150:intron 25.0%
! ATTTATAGTAGTATGTTTGG+GGG + chr2_3:55517208-55517227 Msa0300150:intron 25.0%
!!! TCTGGTTTTTGAGATTATAT+TGG + chr2_3:55517335-55517354 Msa0300150:intron 25.0%
! TTGTAACATTAAACAAGAGA+AGG - chr2_3:55517540-55517559 None:intergenic 25.0%
!! TCTCTTGTTTAATGTTACAA+CGG + chr2_3:55517540-55517559 Msa0300150:intron 25.0%
!! TTTAAACTGTTGTTATTGCT+AGG + chr2_3:55517648-55517667 Msa0300150:intron 25.0%
!! TTAAACTGTTGTTATTGCTA+GGG + chr2_3:55517649-55517668 Msa0300150:intron 25.0%
! GATATTAATACAGACTAACA+TGG - chr2_3:55517706-55517725 None:intergenic 25.0%
!! ATTAATTGGTACATCTTTTG+AGG + chr2_3:55518310-55518329 Msa0300150:three_prime_UTR 25.0%
!!! ATTTTGGTCTATTTTATGGT+TGG + chr2_3:55518455-55518474 Msa0300150:three_prime_UTR 25.0%
! ATTATTCAACTTCATGGTAT+GGG + chr2_3:55518559-55518578 Msa0300150:three_prime_UTR 25.0%
! CACAAATATATGAATGCTTA+TGG - chr2_3:55518610-55518629 None:intergenic 25.0%
! TTGTTATTAGGGTTCTTATC+AGG - chr2_3:55515311-55515330 None:intergenic 30.0%
AGAGCTGTTTATGATCAATA+TGG + chr2_3:55515530-55515549 Msa0300150:CDS 30.0%
GAGCTGTTTATGATCAATAT+GGG + chr2_3:55515531-55515550 Msa0300150:CDS 30.0%
AGCTGTTTATGATCAATATG+GGG + chr2_3:55515532-55515551 Msa0300150:CDS 30.0%
AACTCAGCAAAAATATCATC+AGG - chr2_3:55515671-55515690 None:intergenic 30.0%
! ACAGCATTGAAAAAAGAACT+AGG - chr2_3:55515940-55515959 None:intergenic 30.0%
ATTGCTGAGATTTACATGTT+TGG + chr2_3:55516030-55516049 Msa0300150:intron 30.0%
! TGTAGCATTTCTAGATTTAC+AGG + chr2_3:55516067-55516086 Msa0300150:intron 30.0%
TAGAAAAGATTGTGGGAAAT+AGG + chr2_3:55516217-55516236 Msa0300150:intron 30.0%
AAGTATTATCTCACTTAGTG+GGG - chr2_3:55516444-55516463 None:intergenic 30.0%
CAAGTATTATCTCACTTAGT+GGG - chr2_3:55516445-55516464 None:intergenic 30.0%
ACAAGTATTATCTCACTTAG+TGG - chr2_3:55516446-55516465 None:intergenic 30.0%
TGGATGAAATAGCAAAAACA+TGG - chr2_3:55516505-55516524 None:intergenic 30.0%
ATCTGCTTCTTTATTGAAAG+AGG + chr2_3:55516596-55516615 Msa0300150:intron 30.0%
ATACTTAAAATTGGAGGAAG+AGG - chr2_3:55516694-55516713 None:intergenic 30.0%
CCATCAATACTTAAAATTGG+AGG - chr2_3:55516700-55516719 None:intergenic 30.0%
!!! CCTCCAATTTTAAGTATTGA+TGG + chr2_3:55516697-55516716 Msa0300150:intron 30.0%
AAACATTCAATCTAACGTGT+CGG - chr2_3:55516860-55516879 None:intergenic 30.0%
CAATATAACTCGCTTCATAT+CGG - chr2_3:55517047-55517066 None:intergenic 30.0%
ATAGACAATAAGTAGCTTCT+TGG + chr2_3:55517135-55517154 Msa0300150:intron 30.0%
AATACTAGATTTGTGATGCT+TGG + chr2_3:55517237-55517256 Msa0300150:intron 30.0%
TGTCATAAAGTTTATGTCTC+TGG + chr2_3:55517317-55517336 Msa0300150:intron 30.0%
TTGCAACATTAAACAAGAGA+AGG - chr2_3:55517443-55517462 None:intergenic 30.0%
TTAAACAAGAGAAGGACAAT+GGG - chr2_3:55517532-55517551 None:intergenic 30.0%
ATTAAACAAGAGAAGGACAA+TGG - chr2_3:55517533-55517552 None:intergenic 30.0%
! AACATCTAGAATTCGAGATA+AGG + chr2_3:55517736-55517755 Msa0300150:intron 30.0%
! ACATCTAGAATTCGAGATAA+GGG + chr2_3:55517737-55517756 Msa0300150:intron 30.0%
ATGCATGATATCTCTCATTA+TGG + chr2_3:55517776-55517795 Msa0300150:intron 30.0%
ATGATGAAAATGATATCAGC+AGG - chr2_3:55517975-55517994 None:intergenic 30.0%
TAGATTACCATAATCTTGAC+AGG + chr2_3:55518359-55518378 Msa0300150:three_prime_UTR 30.0%
ATTGGGAAATTTGGTTGTTA+TGG + chr2_3:55518387-55518406 Msa0300150:three_prime_UTR 30.0%
GTTAGACAATGAGTTACATT+GGG + chr2_3:55518477-55518496 Msa0300150:three_prime_UTR 30.0%
ACCAACATTATTCAACTTCA+TGG + chr2_3:55518553-55518572 Msa0300150:three_prime_UTR 30.0%
ACCATGAAGTTGAATAATGT+TGG - chr2_3:55518557-55518576 None:intergenic 30.0%
CATTATTCAACTTCATGGTA+TGG + chr2_3:55518558-55518577 Msa0300150:three_prime_UTR 30.0%
TTATTCAACTTCATGGTATG+GGG + chr2_3:55518560-55518579 Msa0300150:three_prime_UTR 30.0%
! CTTTCTCTTCGACAGTTTTA+CGG - chr2_3:55515171-55515190 None:intergenic 35.0%
TTTCTTCAAGTCATCGTCTT+TGG - chr2_3:55515258-55515277 None:intergenic 35.0%
TATGATCAATATGGGGAAGA+TGG + chr2_3:55515539-55515558 Msa0300150:CDS 35.0%
ATGATCAATATGGGGAAGAT+GGG + chr2_3:55515540-55515559 Msa0300150:CDS 35.0%
TTCCGTATCAGTTCAATCAT+CGG + chr2_3:55515642-55515661 Msa0300150:CDS 35.0%
! CATAGCAACTTGTTCTCAAT+AGG - chr2_3:55515824-55515843 None:intergenic 35.0%
ACTGTGATCAAGAATTTCTC+TGG - chr2_3:55515894-55515913 None:intergenic 35.0%
AGAGAAATTCTTGATCACAG+TGG + chr2_3:55515893-55515912 Msa0300150:CDS 35.0%
GAGAAATTCTTGATCACAGT+GGG + chr2_3:55515894-55515913 Msa0300150:CDS 35.0%
CATTGAAAAAAGAACTAGGC+AGG - chr2_3:55515936-55515955 None:intergenic 35.0%
AAAGTAGCTCAGTTTCAGTT+TGG + chr2_3:55515994-55516013 Msa0300150:intron 35.0%
TAATTGGAGAAGAGACTGAT+TGG + chr2_3:55516119-55516138 Msa0300150:intron 35.0%
ACCCATAGTAGAAAAGATTG+TGG + chr2_3:55516209-55516228 Msa0300150:intron 35.0%
! CCCACAATCTTTTCTACTAT+GGG - chr2_3:55516213-55516232 None:intergenic 35.0%
CCCATAGTAGAAAAGATTGT+GGG + chr2_3:55516210-55516229 Msa0300150:intron 35.0%
! TCCCACAATCTTTTCTACTA+TGG - chr2_3:55516214-55516233 None:intergenic 35.0%
ACCTGTACTGTAGATTATGT+AGG + chr2_3:55516269-55516288 Msa0300150:intron 35.0%
TCCTACATAATCTACAGTAC+AGG - chr2_3:55516273-55516292 None:intergenic 35.0%
GTACTGTAGATTATGTAGGA+AGG + chr2_3:55516273-55516292 Msa0300150:intron 35.0%
TTAAAATTGGAGGAAGAGGA+AGG - chr2_3:55516690-55516709 None:intergenic 35.0%
GTGTGACATGTATCACTATA+TGG + chr2_3:55516889-55516908 Msa0300150:intron 35.0%
TGTCTATGTCAATGCTTCAT+AGG + chr2_3:55517012-55517031 Msa0300150:intron 35.0%
CAATGCTTCATAGGTTAACA+TGG + chr2_3:55517021-55517040 Msa0300150:intron 35.0%
CGATATGAAGCGAGTTATAT+TGG + chr2_3:55517045-55517064 Msa0300150:intron 35.0%
TCCTATTTCATTACAGTCCA+GGG + chr2_3:55517358-55517377 Msa0300150:intron 35.0%
TGTTGCAAATTACAATGGCT+TGG + chr2_3:55517455-55517474 Msa0300150:intron 35.0%
!! ATGGCTTGGTGTTTGTTAAA+CGG + chr2_3:55517469-55517488 Msa0300150:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG + chr2_3:55517482-55517501 Msa0300150:intron 35.0%
TAAACAAGAGAAGGACAATG+GGG - chr2_3:55517531-55517550 None:intergenic 35.0%
TGTTTAATGTTACAACGGCT+TGG + chr2_3:55517545-55517564 Msa0300150:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG + chr2_3:55517482-55517501 Msa0300150:intron 35.0%
GGGGTTATTACATTAGACTA+AGG - chr2_3:55517602-55517621 None:intergenic 35.0%
TCCCAAACAATATGCACAAT+GGG - chr2_3:55517622-55517641 None:intergenic 35.0%
ATCCCAAACAATATGCACAA+TGG - chr2_3:55517623-55517642 None:intergenic 35.0%
ATTGTGCATATTGTTTGGGA+TGG + chr2_3:55517622-55517641 Msa0300150:intron 35.0%
TTGACAATTAGTGTCAAACC+TGG + chr2_3:55517888-55517907 Msa0300150:CDS 35.0%
ATATCAGCAGGTATGGTATT+TGG - chr2_3:55517963-55517982 None:intergenic 35.0%
GAAAATGATATCAGCAGGTA+TGG - chr2_3:55517970-55517989 None:intergenic 35.0%
ATTACCATAATCTTGACAGG+AGG + chr2_3:55518362-55518381 Msa0300150:three_prime_UTR 35.0%
GGTTAGACAATGAGTTACAT+TGG + chr2_3:55518476-55518495 Msa0300150:three_prime_UTR 35.0%
! TTTTGCGGCGAATTTGTGAA+GGG - chr2_3:55515122-55515141 None:intergenic 40.0%
! ATTTTGCGGCGAATTTGTGA+AGG - chr2_3:55515123-55515142 None:intergenic 40.0%
! TCCGATTTTCTGCGATTTTG+CGG - chr2_3:55515137-55515156 None:intergenic 40.0%
TGTCGAAGAGAAAGAGAGAA+GGG + chr2_3:55515177-55515196 Msa0300150:five_prime_UTR 40.0%
AACTGATACGGAAAGTCATC+AGG - chr2_3:55515635-55515654 None:intergenic 40.0%
GTCCGATGATTGAACTGATA+CGG - chr2_3:55515647-55515666 None:intergenic 40.0%
AATGGCATGTTTGGATCCTA+TGG + chr2_3:55515743-55515762 Msa0300150:CDS 40.0%
! AGCAACTTGTTCTCAATAGG+AGG - chr2_3:55515821-55515840 None:intergenic 40.0%
CAAGTTGCTATGTTCTCTTG+AGG + chr2_3:55515832-55515851 Msa0300150:CDS 40.0%
GGTGATCTTCATCTTCTTAG+TGG - chr2_3:55515873-55515892 None:intergenic 40.0%
! ACAAACCCTCGTGGATTAAT+TGG + chr2_3:55516103-55516122 Msa0300150:intron 40.0%
CTTCTCCAATTAATCCACGA+GGG - chr2_3:55516111-55516130 None:intergenic 40.0%
TCTTCTCCAATTAATCCACG+AGG - chr2_3:55516112-55516131 None:intergenic 40.0%
!! CTGATTGGTCTCTTATAGCT+CGG + chr2_3:55516134-55516153 Msa0300150:intron 40.0%
AGATTGTGGGAAATAGGCTT+AGG + chr2_3:55516223-55516242 Msa0300150:intron 40.0%
GAAATAGGCTTAGGTGGTTT+AGG + chr2_3:55516232-55516251 Msa0300150:intron 40.0%
GAGGATAGTCAAATCACTAG+AGG + chr2_3:55516311-55516330 Msa0300150:intron 40.0%
ATTATCTCACTTAGTGGGGT+TGG - chr2_3:55516440-55516459 None:intergenic 40.0%
CCAGCATATTCTCTACAACA+TGG - chr2_3:55516525-55516544 None:intergenic 40.0%
CCATGTTGTAGAGAATATGC+TGG + chr2_3:55516522-55516541 Msa0300150:intron 40.0%
GTTGTAGAGAATATGCTGGT+AGG + chr2_3:55516526-55516545 Msa0300150:intron 40.0%
!! GTAGGTTTTGTAGTTGTGGT+TGG + chr2_3:55516544-55516563 Msa0300150:intron 40.0%
! CTGAGTTTTACACTCAAGTC+AGG + chr2_3:55516738-55516757 Msa0300150:intron 40.0%
!! TGAAGCATTGACATAGACAC+CGG - chr2_3:55517011-55517030 None:intergenic 40.0%
GTCCTATTTCATTACAGTCC+AGG + chr2_3:55517357-55517376 Msa0300150:intron 40.0%
TCCCTGGACTGTAATGAAAT+AGG - chr2_3:55517362-55517381 None:intergenic 40.0%
!! TGGCTTGGTGTTTGTTAAAC+GGG + chr2_3:55517470-55517489 Msa0300150:intron 40.0%
! GGGGTTAGTACATTAGACTA+AGG - chr2_3:55517512-55517531 None:intergenic 40.0%
!! ACGGCTTGGTGTTTGTTAAA+CGG + chr2_3:55517559-55517578 Msa0300150:intron 40.0%
ACCCCATTGTGCATATTGTT+TGG + chr2_3:55517617-55517636 Msa0300150:intron 40.0%
CCCAAACAATATGCACAATG+GGG - chr2_3:55517621-55517640 None:intergenic 40.0%
CCCCATTGTGCATATTGTTT+GGG + chr2_3:55517618-55517637 Msa0300150:intron 40.0%
CAATTAGTGTCAAACCTGGA+TGG + chr2_3:55517892-55517911 Msa0300150:CDS 40.0%
TGGATGTTCATTTCCCTTCT+CGG - chr2_3:55517943-55517962 None:intergenic 40.0%
ATTCACTGTACAACCAGCTA+AGG - chr2_3:55518075-55518094 None:intergenic 40.0%
GTGAATCTCACTACACTTGA+TGG + chr2_3:55518089-55518108 Msa0300150:CDS 40.0%
TGTTGTTCATCCTGAGTATG+AGG + chr2_3:55518139-55518158 Msa0300150:CDS 40.0%
CCTTCTTTGTTGGATCCTTT+GGG - chr2_3:55518193-55518212 None:intergenic 40.0%
CCAAAGGATCCAACAAAGAA+GGG + chr2_3:55518191-55518210 Msa0300150:CDS 40.0%
CTCAAGTTTCCCTTCTTTGT+TGG - chr2_3:55518203-55518222 None:intergenic 40.0%
CAACAAAGAAGGGAAACTTG+AGG + chr2_3:55518201-55518220 Msa0300150:CDS 40.0%
TCAACGTCAAATTCCCAACT+CGG + chr2_3:55518231-55518250 Msa0300150:CDS 40.0%
ATTGCCTCCTGTCAAGATTA+TGG - chr2_3:55518369-55518388 None:intergenic 40.0%
TAATCTTGACAGGAGGCAAT+TGG + chr2_3:55518369-55518388 Msa0300150:three_prime_UTR 40.0%
AATCTTGACAGGAGGCAATT+GGG + chr2_3:55518370-55518389 Msa0300150:three_prime_UTR 40.0%
GCCGCAAAATCGCAGAAAAT+CGG + chr2_3:55515133-55515152 Msa0300150:five_prime_UTR 45.0%
! GACAGTTTTACGGCACTTTC+TGG - chr2_3:55515161-55515180 None:intergenic 45.0%
CTGTCGAAGAGAAAGAGAGA+AGG + chr2_3:55515176-55515195 Msa0300150:five_prime_UTR 45.0%
CTTGTAGTAGTCAACACCCA+TGG - chr2_3:55515213-55515232 None:intergenic 45.0%
TGACTACTACAAGCTTCTGC+AGG + chr2_3:55515220-55515239 Msa0300150:CDS 45.0%
!! ACAAGCTTCTGCAGGTTGAT+CGG + chr2_3:55515228-55515247 Msa0300150:CDS 45.0%
ACAGAAAACTCGCCATGAAG+TGG + chr2_3:55515282-55515301 Msa0300150:CDS 45.0%
! CTTATCAGGATGCCACTTCA+TGG - chr2_3:55515297-55515316 None:intergenic 45.0%
!!! TCTGGGTCATGCTGATTTTG+TGG + chr2_3:55515462-55515481 Msa0300150:intron 45.0%
!!! GGTCATGCTGATTTTGTGGT+GGG + chr2_3:55515466-55515485 Msa0300150:intron 45.0%
TTTGGGGATCGAAATCCCTT+TGG + chr2_3:55515692-55515711 Msa0300150:CDS 45.0%
TCGAAATCCCTTTGGTGGAA+TGG + chr2_3:55515700-55515719 Msa0300150:CDS 45.0%
CGAAATCCCTTTGGTGGAAT+GGG + chr2_3:55515701-55515720 Msa0300150:CDS 45.0%
AGAGGTAGCAATGGCATGTT+TGG + chr2_3:55515734-55515753 Msa0300150:CDS 45.0%
! TTTGGATCCTATGGTGATGC+TGG + chr2_3:55515752-55515771 Msa0300150:CDS 45.0%
AGTGGAGTCAATGTGCATCA+AGG + chr2_3:55515779-55515798 Msa0300150:CDS 45.0%
AACCAAAGTACAAACCCTCG+TGG + chr2_3:55516094-55516113 Msa0300150:intron 45.0%
ATCCACGAGGGTTTGTACTT+TGG - chr2_3:55516099-55516118 None:intergenic 45.0%
TTGTGGGAAATAGGCTTAGG+TGG + chr2_3:55516226-55516245 Msa0300150:intron 45.0%
AGGAAGGACAGTAGATGACA+TGG + chr2_3:55516289-55516308 Msa0300150:intron 45.0%
AAGGACAGTAGATGACATGG+AGG + chr2_3:55516292-55516311 Msa0300150:intron 45.0%
AGTCAAATCACTAGAGGCAG+AGG + chr2_3:55516317-55516336 Msa0300150:intron 45.0%
!! GCTGGTAGGTTTTGTAGTTG+TGG + chr2_3:55516540-55516559 Msa0300150:intron 45.0%
! TGTGGTTGGTGCAATGTATG+TGG + chr2_3:55516558-55516577 Msa0300150:intron 45.0%
!! TGTATGTGGTGCCATTTGTG+TGG + chr2_3:55516572-55516591 Msa0300150:intron 45.0%
AGAAGCAGATGCCACACAAA+TGG - chr2_3:55516586-55516605 None:intergenic 45.0%
!! CTATATGGCATCGACACATG+TGG + chr2_3:55516904-55516923 Msa0300150:intron 45.0%
TGACACAGACACTTTGACAC+TGG - chr2_3:55516973-55516992 None:intergenic 45.0%
! CAAAGTGTCTGTGTCATGTC+TGG + chr2_3:55516977-55516996 Msa0300150:intron 45.0%
AGGGATGTAGAAGTCTTCTG+CGG + chr2_3:55517377-55517396 Msa0300150:intron 45.0%
!! CGGCTTGGTGTTTGTTAAAC+GGG + chr2_3:55517560-55517579 Msa0300150:intron 45.0%
TGTCAAACCTGGATGGAAGA+AGG + chr2_3:55517899-55517918 Msa0300150:CDS 45.0%
GTCAAACCTGGATGGAAGAA+GGG + chr2_3:55517900-55517919 Msa0300150:CDS 45.0%
TCAAACCTGGATGGAAGAAG+GGG + chr2_3:55517901-55517920 Msa0300150:CDS 45.0%
AAAGATCACTTTCCCCGAGA+AGG + chr2_3:55517926-55517945 Msa0300150:CDS 45.0%
AAGATCACTTTCCCCGAGAA+GGG + chr2_3:55517927-55517946 Msa0300150:CDS 45.0%
GATGTTCATTTCCCTTCTCG+GGG - chr2_3:55517941-55517960 None:intergenic 45.0%
GGATGTTCATTTCCCTTCTC+GGG - chr2_3:55517942-55517961 None:intergenic 45.0%
TCACGAGTGAACACATTGTG+TGG - chr2_3:55518008-55518027 None:intergenic 45.0%
CACAATGTGTTCACTCGTGA+AGG + chr2_3:55518008-55518027 Msa0300150:CDS 45.0%
TGTTCATCCTGAGTATGAGG+AGG + chr2_3:55518142-55518161 Msa0300150:CDS 45.0%
! TTTGTTGGATCCTTTGGGAG+TGG - chr2_3:55518188-55518207 None:intergenic 45.0%
CCCAAAGGATCCAACAAAGA+AGG + chr2_3:55518190-55518209 Msa0300150:CDS 45.0%
CCCTTCTTTGTTGGATCCTT+TGG - chr2_3:55518194-55518213 None:intergenic 45.0%
GGCAGGAATGAAGAAAGTCT+TGG + chr2_3:55518271-55518290 Msa0300150:CDS 45.0%
GGAATGAAGAAAGTCTTGGC+TGG + chr2_3:55518275-55518294 Msa0300150:CDS 45.0%
CAGGAGGCAATTGGGAAATT+TGG + chr2_3:55518378-55518397 Msa0300150:three_prime_UTR 45.0%
TAGTAGTCAACACCCATGGC+AGG - chr2_3:55515209-55515228 None:intergenic 50.0%
!!! GGGTCATGCTGATTTTGTGG+TGG + chr2_3:55515465-55515484 Msa0300150:intron 50.0%
!! ATATGGGGAAGATGGGCTGA+AGG + chr2_3:55515547-55515566 Msa0300150:CDS 50.0%
!! TATGGGGAAGATGGGCTGAA+GGG + chr2_3:55515548-55515567 Msa0300150:CDS 50.0%
! CTACCAGAGGCATGAGAATC+AGG - chr2_3:55515590-55515609 None:intergenic 50.0%
GATTCTCATGCCTCTGGTAG+TGG + chr2_3:55515590-55515609 Msa0300150:CDS 50.0%
TCTACTACCACCACTACCAG+AGG - chr2_3:55515603-55515622 None:intergenic 50.0%
AATCCCTTTGGTGGAATGGG+AGG + chr2_3:55515704-55515723 Msa0300150:CDS 50.0%
ATCCCTTTGGTGGAATGGGA+GGG + chr2_3:55515705-55515724 Msa0300150:CDS 50.0%
ATCCCTCCCATTCCACCAAA+GGG - chr2_3:55515710-55515729 None:intergenic 50.0%
TTTGGTGGAATGGGAGGGAT+GGG + chr2_3:55515710-55515729 Msa0300150:CDS 50.0%
ACTTCCTCCAGCATCACCAT+AGG - chr2_3:55515762-55515781 None:intergenic 50.0%
! TATGGTGATGCTGGAGGAAG+TGG + chr2_3:55515761-55515780 Msa0300150:CDS 50.0%
ACTAGAGGCAGAGGAAGACT+TGG + chr2_3:55516326-55516345 Msa0300150:intron 50.0%
!! ACTTAGTGGGGTTGGCTACA+TGG - chr2_3:55516432-55516451 None:intergenic 50.0%
GCAGAAGACTTCTACATCCC+TGG - chr2_3:55517378-55517397 None:intergenic 50.0%
GTGACTTTGCTGCACTCGAA+AGG - chr2_3:55517852-55517871 None:intergenic 50.0%
TTTGTCCCCTTCTTCCATCC+AGG - chr2_3:55517909-55517928 None:intergenic 50.0%
TCTCTTGCAGAAGCCTTAGC+TGG + chr2_3:55518059-55518078 Msa0300150:CDS 50.0%
GGAACAACCTCCTCATACTC+AGG - chr2_3:55518152-55518171 None:intergenic 50.0%
GAGGAGGTTGTTCCAAGAGA+AGG + chr2_3:55518158-55518177 Msa0300150:CDS 50.0%
AGAAGGAATGCCACTCCCAA+AGG + chr2_3:55518175-55518194 Msa0300150:CDS 50.0%
ATTCCCAACTCGGCTCACTT+CGG + chr2_3:55518241-55518260 Msa0300150:CDS 50.0%
AGAAGGGCAACAACCTGCCA+TGG + chr2_3:55515193-55515212 Msa0300150:exon 55.0%
GAAGGGCAACAACCTGCCAT+GGG + chr2_3:55515194-55515213 Msa0300150:exon 55.0%
! GCATGAGAATCAGGTGGTGG+TGG - chr2_3:55515581-55515600 None:intergenic 55.0%
GAGGCATGAGAATCAGGTGG+TGG - chr2_3:55515584-55515603 None:intergenic 55.0%
! CCAGAGGCATGAGAATCAGG+TGG - chr2_3:55515587-55515606 None:intergenic 55.0%
CCACCTGATTCTCATGCCTC+TGG + chr2_3:55515584-55515603 Msa0300150:CDS 55.0%
!! TCTCATGCCTCTGGTAGTGG+TGG + chr2_3:55515593-55515612 Msa0300150:CDS 55.0%
GGGGATCGAAATCCCTTTGG+TGG + chr2_3:55515695-55515714 Msa0300150:CDS 55.0%
CATCCCTCCCATTCCACCAA+AGG - chr2_3:55515711-55515730 None:intergenic 55.0%
CTTTGGTGGAATGGGAGGGA+TGG + chr2_3:55515709-55515728 Msa0300150:CDS 55.0%
GGGATGGGAAGAGGTAGCAA+TGG + chr2_3:55515725-55515744 Msa0300150:CDS 55.0%
! GGATCCTATGGTGATGCTGG+AGG + chr2_3:55515755-55515774 Msa0300150:CDS 55.0%
GCATCAAGGTCCTCCTCGTA+AGG + chr2_3:55515793-55515812 Msa0300150:CDS 55.0%
!! TCAATAGGAGGCGCCTTACG+AGG - chr2_3:55515809-55515828 None:intergenic 55.0%
GGAAGAGGAAGGTTCGACTG+CGG - chr2_3:55516679-55516698 None:intergenic 55.0%
!! GTCATGTCTGGTGTCGTGTC+CGG + chr2_3:55516989-55517008 Msa0300150:intron 55.0%
GTGCAGCAAAGTCACATCGC+TGG + chr2_3:55517857-55517876 Msa0300150:intron 55.0%
GGGAGTGGCATTCCTTCTCT+TGG - chr2_3:55518173-55518192 None:intergenic 55.0%
GATCCGAAGTGAGCCGAGTT+GGG - chr2_3:55518247-55518266 None:intergenic 55.0%
TGATCCGAAGTGAGCCGAGT+TGG - chr2_3:55518248-55518267 None:intergenic 55.0%
TCGGCTCACTTCGGATCAGA+AGG + chr2_3:55518250-55518269 Msa0300150:CDS 55.0%
CTCACTTCGGATCAGAAGGC+AGG + chr2_3:55518254-55518273 Msa0300150:CDS 55.0%
GGAATGGGAGGGATGGGAAG+AGG + chr2_3:55515716-55515735 Msa0300150:CDS 60.0%
!! ATAGGAGGCGCCTTACGAGG+AGG - chr2_3:55515806-55515825 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55515056 55518629 55515056 ID=Msa0300150;Name=Msa0300150
chr2_3 mRNA 55515056 55518629 55515056 ID=Msa0300150-mRNA-1;Parent=Msa0300150;Name=Msa0300150-mRNA-1;_AED=0.06;_eAED=0.06;_QI=156|1|1|1|1|1|3|328|336
chr2_3 exon 55515056 55515376 55515056 ID=Msa0300150-mRNA-1:exon:17758;Parent=Msa0300150-mRNA-1
chr2_3 exon 55515509 55515915 55515509 ID=Msa0300150-mRNA-1:exon:17759;Parent=Msa0300150-mRNA-1
chr2_3 exon 55517863 55518629 55517863 ID=Msa0300150-mRNA-1:exon:17760;Parent=Msa0300150-mRNA-1
chr2_3 five_prime_UTR 55515056 55515211 55515056 ID=Msa0300150-mRNA-1:five_prime_utr;Parent=Msa0300150-mRNA-1
chr2_3 CDS 55515212 55515376 55515212 ID=Msa0300150-mRNA-1:cds;Parent=Msa0300150-mRNA-1
chr2_3 CDS 55515509 55515915 55515509 ID=Msa0300150-mRNA-1:cds;Parent=Msa0300150-mRNA-1
chr2_3 CDS 55517863 55518301 55517863 ID=Msa0300150-mRNA-1:cds;Parent=Msa0300150-mRNA-1
chr2_3 three_prime_UTR 55518302 55518629 55518302 ID=Msa0300150-mRNA-1:three_prime_utr;Parent=Msa0300150-mRNA-1
Gene Sequence

>Msa0300150

ATGGGTGTTGACTACTACAAGCTTCTGCAGGTTGATCGGAACGCCAAAGACGATGACTTGAAGAAAGCTTACAGAAAACTCGCCATGAAGTGGCATCCTGATAAGAACCCTAATAACAAAAAAGAAGCTGAAGCCAAATTTAAGCAAATCTCAGAAGCTTATGATGTATTGAGTGATTCTAACAAGAGAGCTGTTTATGATCAATATGGGGAAGATGGGCTGAAGGGTCAAATGCCACCACCACCTGATTCTCATGCCTCTGGTAGTGGTGGTAGTAGAAACTTTTCACCTGATGACTTTCCGTATCAGTTCAATCATCGGACTCCTGATGATATTTTTGCTGAGTTTTTTGGGGATCGAAATCCCTTTGGTGGAATGGGAGGGATGGGAAGAGGTAGCAATGGCATGTTTGGATCCTATGGTGATGCTGGAGGAAGTGGAGTCAATGTGCATCAAGGTCCTCCTCGTAAGGCGCCTCCTATTGAGAACAAGTTGCTATGTTCTCTTGAGGATATATATAAAGGGACCACTAAGAAGATGAAGATCACCAGAGAAATTCTTGATCACAGTGGCAAAGTCACATCGCTGGATGAAATCTTGACAATTAGTGTCAAACCTGGATGGAAGAAGGGGACAAAGATCACTTTCCCCGAGAAGGGAAATGAACATCCAAATACCATACCTGCTGATATCATTTTCATCATCGATGAAAAACCACACAATGTGTTCACTCGTGAAGGAAATGATCTAATTGTAACTCAGAAAATTTCTCTTGCAGAAGCCTTAGCTGGTTGTACAGTGAATCTCACTACACTTGATGGCAGAAATTTGACTGTAGTGATCAACAATGTTGTTCATCCTGAGTATGAGGAGGTTGTTCCAAGAGAAGGAATGCCACTCCCAAAGGATCCAACAAAGAAGGGAAACTTGAGGATAAAGTTCAACGTCAAATTCCCAACTCGGCTCACTTCGGATCAGAAGGCAGGAATGAAGAAAGTCTTGGCTGGATAA
Protein sequence

>Msa0300150

MGVDYYKLLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDSNKRAVYDQYGEDGLKGQMPPPPDSHASGSGGSRNFSPDDFPYQFNHRTPDDIFAEFFGDRNPFGGMGGMGRGSNGMFGSYGDAGGSGVNVHQGPPRKAPPIENKLLCSLEDIYKGTTKKMKITREILDHSGKVTSLDEILTISVKPGWKKGTKITFPEKGNEHPNTIPADIIFIIDEKPHNVFTREGNDLIVTQKISLAEALAGCTVNLTTLDGRNLTVVINNVVHPEYEEVVPREGMPLPKDPTKKGNLRIKFNVKFPTRLTSDQKAGMKKVLAG*