AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0487800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATTGTTTATGTTTAT+AGG 0.265507 3_4:+6935333 Msa0487800:CDS
TCGTTGAACATTACATTTAG+AGG 0.314922 3_4:+6935305 Msa0487800:CDS
ATCAACTGCTGGAGCGGAAC+AGG 0.386495 3_4:+6935036 Msa0487800:CDS
TAGCAAGTTGAAAATGATAA+AGG 0.409340 3_4:+6935059 Msa0487800:CDS
CTCACTGGTTGAAAACTCTC+AGG 0.472665 3_4:-6934398 None:intergenic
AAGGAAGGAACATCAACTGC+TGG 0.537273 3_4:+6935025 Msa0487800:CDS
ATGATAAAGGTAAGAAGCGA+TGG 0.555569 3_4:+6935072 Msa0487800:CDS
ACAAAGGATACAAGGCAAGA+AGG 0.567711 3_4:+6935006 Msa0487800:CDS
AGGATACAAGGCAAGAAGGA+AGG 0.592550 3_4:+6935010 Msa0487800:CDS
AGGAACATCAACTGCTGGAG+CGG 0.594264 3_4:+6935030 Msa0487800:CDS
AGGTGATGACAAAGGATACA+AGG 0.600639 3_4:+6934998 Msa0487800:intron
TTTGTATCAAAATTGTGCAG+AGG 0.627197 3_4:+6935166 Msa0487800:CDS
TAGAAGCTGTGACAGCAAAG+TGG 0.630311 3_4:+6935098 Msa0487800:CDS
GTGCTCTCAGGTGATGACAA+AGG 0.665545 3_4:+6934990 Msa0487800:intron
TAGGACAACAATGAGCAATG+AGG 0.709913 3_4:+6934820 Msa0487800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATAAAATCCAAAATTT+TGG + chr3_4:6934599-6934618 Msa0487800:intron 15.0%
!!! TTGAATAAAATCCAAAATTT+TGG + chr3_4:6934599-6934618 Msa0487800:intron 15.0%
!!! AATCTATGAATTCATTTTCA+TGG - chr3_4:6934539-6934558 None:intergenic 20.0%
!!! GTTTTAATTACTTAGTTGAA+TGG + chr3_4:6934563-6934582 Msa0487800:intron 20.0%
!!! ATTTTGGATTTTATTCAAAC+TGG - chr3_4:6934597-6934616 None:intergenic 20.0%
!! TAATTCCTTTGATCAAAATA+TGG + chr3_4:6934886-6934905 Msa0487800:intron 20.0%
!! AATTCCTTTGATCAAAATAT+GGG + chr3_4:6934887-6934906 Msa0487800:intron 20.0%
!!! GTTTTTTTTTCTTTAAGCAT+AGG + chr3_4:6935261-6935280 Msa0487800:intron 20.0%
!! AACTGATTGTTTATGTTTAT+AGG + chr3_4:6935333-6935352 Msa0487800:CDS 20.0%
!!! AATCTATGAATTCATTTTCA+TGG - chr3_4:6934539-6934558 None:intergenic 20.0%
!!! GTTTTAATTACTTAGTTGAA+TGG + chr3_4:6934563-6934582 Msa0487800:intron 20.0%
!!! ATTTTGGATTTTATTCAAAC+TGG - chr3_4:6934597-6934616 None:intergenic 20.0%
!! TAATTCCTTTGATCAAAATA+TGG + chr3_4:6934886-6934905 Msa0487800:intron 20.0%
!! AATTCCTTTGATCAAAATAT+GGG + chr3_4:6934887-6934906 Msa0487800:intron 20.0%
!!! GTTTTTTTTTCTTTAAGCAT+AGG + chr3_4:6935261-6935280 Msa0487800:intron 20.0%
!! AACTGATTGTTTATGTTTAT+AGG + chr3_4:6935333-6935352 Msa0487800:CDS 20.0%
! ATAGTAGAAGACATAAAAGT+TGG - chr3_4:6934479-6934498 None:intergenic 25.0%
! TCAAAATAAAATCACATCGA+AGG + chr3_4:6934653-6934672 Msa0487800:intron 25.0%
!!! TTAAAGAGAGGAATTTTTAG+CGG + chr3_4:6934684-6934703 Msa0487800:intron 25.0%
! GAACAATTTGCAAATGTATT+AGG + chr3_4:6934857-6934876 Msa0487800:intron 25.0%
! AATTTGCAAATGTATTAGGT+GGG + chr3_4:6934861-6934880 Msa0487800:intron 25.0%
! ATTCCTTTGATCAAAATATG+GGG + chr3_4:6934888-6934907 Msa0487800:intron 25.0%
! TGTTATTGTAATATGAGGAA+TGG + chr3_4:6934953-6934972 Msa0487800:intron 25.0%
! GTTATTGTAATATGAGGAAT+GGG + chr3_4:6934954-6934973 Msa0487800:intron 25.0%
!! TAGCAAGTTGAAAATGATAA+AGG + chr3_4:6935059-6935078 Msa0487800:CDS 25.0%
! ATAGTAGAAGACATAAAAGT+TGG - chr3_4:6934479-6934498 None:intergenic 25.0%
! TCAAAATAAAATCACATCGA+AGG + chr3_4:6934653-6934672 Msa0487800:intron 25.0%
!!! TTAAAGAGAGGAATTTTTAG+CGG + chr3_4:6934684-6934703 Msa0487800:intron 25.0%
! GAACAATTTGCAAATGTATT+AGG + chr3_4:6934857-6934876 Msa0487800:intron 25.0%
! AATTTGCAAATGTATTAGGT+GGG + chr3_4:6934861-6934880 Msa0487800:intron 25.0%
! ATTCCTTTGATCAAAATATG+GGG + chr3_4:6934888-6934907 Msa0487800:intron 25.0%
! TGTTATTGTAATATGAGGAA+TGG + chr3_4:6934953-6934972 Msa0487800:intron 25.0%
! GTTATTGTAATATGAGGAAT+GGG + chr3_4:6934954-6934973 Msa0487800:intron 25.0%
!! TAGCAAGTTGAAAATGATAA+AGG + chr3_4:6935059-6935078 Msa0487800:CDS 25.0%
AAGGATTATCTGTTAAAGAG+AGG + chr3_4:6934672-6934691 Msa0487800:intron 30.0%
!!! TAAAGAGAGGAATTTTTAGC+GGG + chr3_4:6934685-6934704 Msa0487800:intron 30.0%
! CAGAGCATTCAAATAATAAG+TGG - chr3_4:6934757-6934776 None:intergenic 30.0%
CAATTTGCAAATGTATTAGG+TGG + chr3_4:6934860-6934879 Msa0487800:intron 30.0%
GAAGAGATAATGCTATAAGT+GGG + chr3_4:6934910-6934929 Msa0487800:intron 30.0%
! AATGCTATAAGTGGGATTTT+TGG + chr3_4:6934918-6934937 Msa0487800:intron 30.0%
! ATGCTATAAGTGGGATTTTT+GGG + chr3_4:6934919-6934938 Msa0487800:intron 30.0%
! GTGGTTGTTATTGTAATATG+AGG + chr3_4:6934948-6934967 Msa0487800:intron 30.0%
TGATACAAAAATCAGTTCAG+TGG + chr3_4:6935139-6935158 Msa0487800:CDS 30.0%
!! CTGCACAATTTTGATACAAA+TGG - chr3_4:6935166-6935185 None:intergenic 30.0%
TTTGTATCAAAATTGTGCAG+AGG + chr3_4:6935166-6935185 Msa0487800:CDS 30.0%
! GGTATGATAATGTATACCAA+TGG + chr3_4:6935187-6935206 Msa0487800:intron 30.0%
TCGTTGAACATTACATTTAG+AGG + chr3_4:6935305-6935324 Msa0487800:CDS 30.0%
AAGGATTATCTGTTAAAGAG+AGG + chr3_4:6934672-6934691 Msa0487800:intron 30.0%
!!! TAAAGAGAGGAATTTTTAGC+GGG + chr3_4:6934685-6934704 Msa0487800:intron 30.0%
! CAGAGCATTCAAATAATAAG+TGG - chr3_4:6934757-6934776 None:intergenic 30.0%
CAATTTGCAAATGTATTAGG+TGG + chr3_4:6934860-6934879 Msa0487800:intron 30.0%
GAAGAGATAATGCTATAAGT+GGG + chr3_4:6934910-6934929 Msa0487800:intron 30.0%
! AATGCTATAAGTGGGATTTT+TGG + chr3_4:6934918-6934937 Msa0487800:intron 30.0%
! ATGCTATAAGTGGGATTTTT+GGG + chr3_4:6934919-6934938 Msa0487800:intron 30.0%
! GTGGTTGTTATTGTAATATG+AGG + chr3_4:6934948-6934967 Msa0487800:intron 30.0%
TGATACAAAAATCAGTTCAG+TGG + chr3_4:6935139-6935158 Msa0487800:CDS 30.0%
!! CTGCACAATTTTGATACAAA+TGG - chr3_4:6935166-6935185 None:intergenic 30.0%
TTTGTATCAAAATTGTGCAG+AGG + chr3_4:6935166-6935185 Msa0487800:CDS 30.0%
! GGTATGATAATGTATACCAA+TGG + chr3_4:6935187-6935206 Msa0487800:intron 30.0%
TCGTTGAACATTACATTTAG+AGG + chr3_4:6935305-6935324 Msa0487800:CDS 30.0%
TGGTAACTGATGTCTTACTT+CGG + chr3_4:6934441-6934460 Msa0487800:intron 35.0%
TTAAGCAGAGTTAGAGTGAA+TGG - chr3_4:6934506-6934525 None:intergenic 35.0%
! CAAGACTTGTGCCAAAATTT+TGG - chr3_4:6934613-6934632 None:intergenic 35.0%
!! CAAAATTTTGGCACAAGTCT+TGG + chr3_4:6934611-6934630 Msa0487800:intron 35.0%
!!! AAAGAGAGGAATTTTTAGCG+GGG + chr3_4:6934686-6934705 Msa0487800:intron 35.0%
ATGCAACCGTATATGTAAGT+GGG + chr3_4:6934709-6934728 Msa0487800:intron 35.0%
TTTGGTGGAGGATTTATTTG+TGG + chr3_4:6934789-6934808 Msa0487800:intron 35.0%
!! TTTATTTGTGGCTTGGTGTT+AGG + chr3_4:6934801-6934820 Msa0487800:intron 35.0%
!!! CTTCCCCATATTTTGATCAA+AGG - chr3_4:6934894-6934913 None:intergenic 35.0%
GGAAGAGATAATGCTATAAG+TGG + chr3_4:6934909-6934928 Msa0487800:intron 35.0%
ATGATAAAGGTAAGAAGCGA+TGG + chr3_4:6935072-6935091 Msa0487800:CDS 35.0%
!! TTTGTTTTCCACTTCAGGAA+AGG - chr3_4:6935233-6935252 None:intergenic 35.0%
TCCTGAAGTGGAAAACAAAA+TGG + chr3_4:6935234-6935253 Msa0487800:intron 35.0%
!!! ACCATTTTGTTTTCCACTTC+AGG - chr3_4:6935238-6935257 None:intergenic 35.0%
TGGTAACTGATGTCTTACTT+CGG + chr3_4:6934441-6934460 Msa0487800:intron 35.0%
TTAAGCAGAGTTAGAGTGAA+TGG - chr3_4:6934506-6934525 None:intergenic 35.0%
! CAAGACTTGTGCCAAAATTT+TGG - chr3_4:6934613-6934632 None:intergenic 35.0%
!! CAAAATTTTGGCACAAGTCT+TGG + chr3_4:6934611-6934630 Msa0487800:intron 35.0%
!!! AAAGAGAGGAATTTTTAGCG+GGG + chr3_4:6934686-6934705 Msa0487800:intron 35.0%
ATGCAACCGTATATGTAAGT+GGG + chr3_4:6934709-6934728 Msa0487800:intron 35.0%
TTTGGTGGAGGATTTATTTG+TGG + chr3_4:6934789-6934808 Msa0487800:intron 35.0%
!! TTTATTTGTGGCTTGGTGTT+AGG + chr3_4:6934801-6934820 Msa0487800:intron 35.0%
!!! CTTCCCCATATTTTGATCAA+AGG - chr3_4:6934894-6934913 None:intergenic 35.0%
GGAAGAGATAATGCTATAAG+TGG + chr3_4:6934909-6934928 Msa0487800:intron 35.0%
ATGATAAAGGTAAGAAGCGA+TGG + chr3_4:6935072-6935091 Msa0487800:CDS 35.0%
!! TTTGTTTTCCACTTCAGGAA+AGG - chr3_4:6935233-6935252 None:intergenic 35.0%
TCCTGAAGTGGAAAACAAAA+TGG + chr3_4:6935234-6935253 Msa0487800:intron 35.0%
!!! ACCATTTTGTTTTCCACTTC+AGG - chr3_4:6935238-6935257 None:intergenic 35.0%
! GAGCTTGCTCTAAGTTCTAT+TGG + chr3_4:6934421-6934440 Msa0487800:intron 40.0%
!! CACAAGTCTTGGTGTTTAGT+TGG + chr3_4:6934622-6934641 Msa0487800:intron 40.0%
GATGCAACCGTATATGTAAG+TGG + chr3_4:6934708-6934727 Msa0487800:intron 40.0%
! GCTCTGTCTTTTCTACAGTT+TGG + chr3_4:6934771-6934790 Msa0487800:intron 40.0%
! CTGTCTTTTCTACAGTTTGG+TGG + chr3_4:6934774-6934793 Msa0487800:intron 40.0%
!! TCTTTTCTACAGTTTGGTGG+AGG + chr3_4:6934777-6934796 Msa0487800:intron 40.0%
TGGAGGATTTATTTGTGGCT+TGG + chr3_4:6934794-6934813 Msa0487800:intron 40.0%
TAGGACAACAATGAGCAATG+AGG + chr3_4:6934820-6934839 Msa0487800:intron 40.0%
AGGTGATGACAAAGGATACA+AGG + chr3_4:6934998-6935017 Msa0487800:intron 40.0%
ACAAAGGATACAAGGCAAGA+AGG + chr3_4:6935006-6935025 Msa0487800:CDS 40.0%
AGCAAAGTGGAGCTAAAAAG+TGG + chr3_4:6935111-6935130 Msa0487800:CDS 40.0%
! TACCAATGGAAGTGTAACTG+AGG + chr3_4:6935201-6935220 Msa0487800:intron 40.0%
! GAGCTTGCTCTAAGTTCTAT+TGG + chr3_4:6934421-6934440 Msa0487800:intron 40.0%
!! CACAAGTCTTGGTGTTTAGT+TGG + chr3_4:6934622-6934641 Msa0487800:intron 40.0%
GATGCAACCGTATATGTAAG+TGG + chr3_4:6934708-6934727 Msa0487800:intron 40.0%
! GCTCTGTCTTTTCTACAGTT+TGG + chr3_4:6934771-6934790 Msa0487800:intron 40.0%
! CTGTCTTTTCTACAGTTTGG+TGG + chr3_4:6934774-6934793 Msa0487800:intron 40.0%
!! TCTTTTCTACAGTTTGGTGG+AGG + chr3_4:6934777-6934796 Msa0487800:intron 40.0%
TGGAGGATTTATTTGTGGCT+TGG + chr3_4:6934794-6934813 Msa0487800:intron 40.0%
TAGGACAACAATGAGCAATG+AGG + chr3_4:6934820-6934839 Msa0487800:intron 40.0%
AGGTGATGACAAAGGATACA+AGG + chr3_4:6934998-6935017 Msa0487800:intron 40.0%
ACAAAGGATACAAGGCAAGA+AGG + chr3_4:6935006-6935025 Msa0487800:CDS 40.0%
AGCAAAGTGGAGCTAAAAAG+TGG + chr3_4:6935111-6935130 Msa0487800:CDS 40.0%
! TACCAATGGAAGTGTAACTG+AGG + chr3_4:6935201-6935220 Msa0487800:intron 40.0%
! CTCACTGGTTGAAAACTCTC+AGG - chr3_4:6934401-6934420 None:intergenic 45.0%
! CTGAGAGTTTTCAACCAGTG+AGG + chr3_4:6934399-6934418 Msa0487800:intron 45.0%
ACTGATGTCTTACTTCGGCA+AGG + chr3_4:6934446-6934465 Msa0487800:intron 45.0%
CTGATGTCTTACTTCGGCAA+GGG + chr3_4:6934447-6934466 Msa0487800:intron 45.0%
CTCGCACCCACTTACATATA+CGG - chr3_4:6934718-6934737 None:intergenic 45.0%
GTGAAGTAGTATGTGCTCTC+AGG + chr3_4:6934978-6934997 Msa0487800:intron 45.0%
AGGATACAAGGCAAGAAGGA+AGG + chr3_4:6935010-6935029 Msa0487800:CDS 45.0%
AAGGAAGGAACATCAACTGC+TGG + chr3_4:6935025-6935044 Msa0487800:CDS 45.0%
TAGAAGCTGTGACAGCAAAG+TGG + chr3_4:6935098-6935117 Msa0487800:CDS 45.0%
CACCTCAGTTACACTTCCAT+TGG - chr3_4:6935206-6935225 None:intergenic 45.0%
! CAATGGAAGTGTAACTGAGG+TGG + chr3_4:6935204-6935223 Msa0487800:intron 45.0%
! CTCACTGGTTGAAAACTCTC+AGG - chr3_4:6934401-6934420 None:intergenic 45.0%
! CTGAGAGTTTTCAACCAGTG+AGG + chr3_4:6934399-6934418 Msa0487800:intron 45.0%
ACTGATGTCTTACTTCGGCA+AGG + chr3_4:6934446-6934465 Msa0487800:intron 45.0%
CTGATGTCTTACTTCGGCAA+GGG + chr3_4:6934447-6934466 Msa0487800:intron 45.0%
CTCGCACCCACTTACATATA+CGG - chr3_4:6934718-6934737 None:intergenic 45.0%
GTGAAGTAGTATGTGCTCTC+AGG + chr3_4:6934978-6934997 Msa0487800:intron 45.0%
AGGATACAAGGCAAGAAGGA+AGG + chr3_4:6935010-6935029 Msa0487800:CDS 45.0%
AAGGAAGGAACATCAACTGC+TGG + chr3_4:6935025-6935044 Msa0487800:CDS 45.0%
TAGAAGCTGTGACAGCAAAG+TGG + chr3_4:6935098-6935117 Msa0487800:CDS 45.0%
CACCTCAGTTACACTTCCAT+TGG - chr3_4:6935206-6935225 None:intergenic 45.0%
! CAATGGAAGTGTAACTGAGG+TGG + chr3_4:6935204-6935223 Msa0487800:intron 45.0%
ACTTAGAGCAAGCTCCTCAC+TGG - chr3_4:6934416-6934435 None:intergenic 50.0%
CGTATATGTAAGTGGGTGCG+AGG + chr3_4:6934716-6934735 Msa0487800:intron 50.0%
!!! TGGGATTTTTGGGTGCGATG+TGG + chr3_4:6934929-6934948 Msa0487800:intron 50.0%
GTGCTCTCAGGTGATGACAA+AGG + chr3_4:6934990-6935009 Msa0487800:intron 50.0%
AGGAACATCAACTGCTGGAG+CGG + chr3_4:6935030-6935049 Msa0487800:CDS 50.0%
ACTTAGAGCAAGCTCCTCAC+TGG - chr3_4:6934416-6934435 None:intergenic 50.0%
CGTATATGTAAGTGGGTGCG+AGG + chr3_4:6934716-6934735 Msa0487800:intron 50.0%
!!! TGGGATTTTTGGGTGCGATG+TGG + chr3_4:6934929-6934948 Msa0487800:intron 50.0%
GTGCTCTCAGGTGATGACAA+AGG + chr3_4:6934990-6935009 Msa0487800:intron 50.0%
AGGAACATCAACTGCTGGAG+CGG + chr3_4:6935030-6935049 Msa0487800:CDS 50.0%
ATCAACTGCTGGAGCGGAAC+AGG + chr3_4:6935036-6935055 Msa0487800:CDS 55.0%
GGTGGTGACCTTTCCTGAAG+TGG + chr3_4:6935222-6935241 Msa0487800:intron 55.0%
ATCAACTGCTGGAGCGGAAC+AGG + chr3_4:6935036-6935055 Msa0487800:CDS 55.0%
GGTGGTGACCTTTCCTGAAG+TGG + chr3_4:6935222-6935241 Msa0487800:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 6934381 6935406 6934381 ID=Msa0487800;Name=Msa0487800
chr3_4 mRNA 6934381 6935406 6934381 ID=Msa0487800-mRNA-1;Parent=Msa0487800;Name=Msa0487800-mRNA-1;_AED=0.95;_eAED=1.00;_QI=0|0|0|0.25|1|1|4|0|131
chr3_4 exon 6934381 6934415 6934381 ID=Msa0487800-mRNA-1:exon:2426;Parent=Msa0487800-mRNA-1
chr3_4 exon 6934823 6934871 6934823 ID=Msa0487800-mRNA-1:exon:2427;Parent=Msa0487800-mRNA-1
chr3_4 exon 6935000 6935187 6935000 ID=Msa0487800-mRNA-1:exon:2428;Parent=Msa0487800-mRNA-1
chr3_4 exon 6935283 6935406 6935283 ID=Msa0487800-mRNA-1:exon:2429;Parent=Msa0487800-mRNA-1
chr3_4 CDS 6934381 6934415 6934381 ID=Msa0487800-mRNA-1:cds;Parent=Msa0487800-mRNA-1
chr3_4 CDS 6934823 6934871 6934823 ID=Msa0487800-mRNA-1:cds;Parent=Msa0487800-mRNA-1
chr3_4 CDS 6935000 6935187 6935000 ID=Msa0487800-mRNA-1:cds;Parent=Msa0487800-mRNA-1
chr3_4 CDS 6935283 6935406 6935283 ID=Msa0487800-mRNA-1:cds;Parent=Msa0487800-mRNA-1
Gene Sequence

>Msa0487800

ATGGGTGATGCTAAGAGCCTGAGAGTTTTCAACCAGACAACAATGAGCAATGAGGCTCAAAAGCACTTTGAACAATTTGCAAATGTGATGACAAAGGATACAAGGCAAGAAGGAAGGAACATCAACTGCTGGAGCGGAACAGGTAGCAAGTTGAAAATGATAAAGGTAAGAAGCGATGGCAATAGAAGCTGTGACAGCAAAGTGGAGCTAAAAAGTGGTACAGTGATACAAAAATCAGTTCAGTGGACCATTTGTATCAAAATTGTGCAGAGGAACAAATTTGCTGCAATATTTTCGTTGAACATTACATTTAGAGGTACTGAACTGATTGTTTATGTTTATAGGAGAAATGATGAGATCAAAATGTTGACTGATGTCGCATACTGTGTTGTATGA

Protein sequence

>Msa0487800

MGDAKSLRVFNQTTMSNEAQKHFEQFANVMTKDTRQEGRNINCWSGTGSKLKMIKVRSDGNRSCDSKVELKSGTVIQKSVQWTICIKIVQRNKFAAIFSLNITFRGTELIVYVYRRNDEIKMLTDVAYCVV*