AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461820 MtrunA17_Chr1g0181961 95.000 140 6 1 1 139 1 140 4.56e-97 276
Msa1461820 MtrunA17_Chr0c01g0489161 94.286 140 8 0 1 140 1 140 2.00e-88 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461820 AT5G02710.3 54.015 137 56 3 9 138 7 143 1.37e-50 159
Msa1461820 AT5G02710.2 54.015 137 56 3 9 138 7 143 1.37e-50 159
Msa1461820 AT5G02710.1 54.015 137 56 3 9 138 7 143 1.37e-50 159

Find 41 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTCTACAATAGCTTAAT+AGG 0.218970 tig0041758:-530 Msa1461820:CDS
TGGGCACTGACCAGAGTAAA+TGG 0.329466 tig0041758:+460 Msa1461820:intergenic
GTTTCCACCGTCTCAAAATC+AGG 0.352865 tig0041758:+1026 Msa1461820:intergenic
GCTTGTTGTAAGCTTCAAAA+AGG 0.360348 tig0041758:-740 Msa1461820:CDS
TAAACAGAAGAATTTCAATA+AGG 0.371896 tig0041758:-287 Msa1461820:CDS
GAGGTGTTTGAATCTTTGTT+TGG 0.380094 tig0041758:-313 Msa1461820:CDS
TTCTTCTTTGCCTTGATAGC+AGG 0.397407 tig0041758:+1004 Msa1461820:intergenic
CATAAGGTAGTAAAATCTAA+TGG 0.411369 tig0041758:+937 Msa1461820:intergenic
GGTGCTGCCGCCATTATCAT+TGG 0.449696 tig0041758:-891 Msa1461820:CDS
GAACATGTGACCAATGATAA+TGG 0.463503 tig0041758:+881 Msa1461820:intergenic
TGGCATTGCGTGCAAGGTTG+TGG 0.476021 tig0041758:-764 Msa1461820:CDS
TCTTCAGGAGATGGATAAGA+AGG 0.485774 tig0041758:+716 Msa1461820:intergenic
CGGCAGCACCAGAGTGGCGG+TGG 0.489404 tig0041758:+904 Msa1461820:intergenic
TAATGTATACACCATCCATC+TGG 0.496715 tig0041758:+506 Msa1461820:intergenic
ATCTCACACCACCGCCACTC+TGG 0.504880 tig0041758:-912 Msa1461820:CDS
CGGTGGTGTGAGATATCATA+AGG 0.511310 tig0041758:+921 Msa1461820:intergenic
ACGGTGGAAACCTGCTATCA+AGG 0.511838 tig0041758:-1014 Msa1461820:five_prime_UTR
TTGTCGTGGCATTGCGTGCA+AGG 0.513920 tig0041758:-770 Msa1461820:CDS
AGGTTTCAGCGGCGAGAAGA+AGG 0.522187 tig0041758:-795 Msa1461820:CDS
AGAAAATGTTCCATTTACTC+TGG 0.525786 tig0041758:-470 Msa1461820:intron
AAACAAAGGCAAAACAATGC+AGG 0.531021 tig0041758:-815 Msa1461820:CDS
TACCGACCCTTCAGATGTTG+AGG 0.536273 tig0041758:-687 Msa1461820:intron
AAATGGACCTCAACATCTGA+AGG 0.540171 tig0041758:+680 Msa1461820:intergenic
ACAGAAGAATTTCAATAAGG+AGG 0.546703 tig0041758:-284 Msa1461820:CDS
ATTTCAATAAGGAGGCATGC+AGG 0.546848 tig0041758:-276 Msa1461820:CDS
AAAATCTAATGGAGTGTGGA+AGG 0.547037 tig0041758:+948 Msa1461820:intergenic
AACAAAGATTCAAACACCTC+AGG 0.567275 tig0041758:+316 Msa1461820:intergenic
TAGTAAAATCTAATGGAGTG+TGG 0.575950 tig0041758:+944 Msa1461820:intergenic
AATAGCTTAATAGGACCAGA+TGG 0.583134 tig0041758:-521 Msa1461820:CDS
CAAAACAATGCAGGTTTCAG+CGG 0.589943 tig0041758:-806 Msa1461820:CDS
GACCTCAACATCTGAAGGGT+CGG 0.594363 tig0041758:+685 Msa1461820:intergenic
ATCATTGGTCACATGTTCAG+CGG 0.607004 tig0041758:-876 Msa1461820:CDS
GCTTAATAGGACCAGATGGA+TGG 0.610306 tig0041758:-517 Msa1461820:CDS
TGGCGGCAGCACCAGAGTGG+CGG 0.617659 tig0041758:+901 Msa1461820:intergenic
GCGTCGTCCACATACAACTA+CGG 0.631589 tig0041758:-852 Msa1461820:CDS
AATGGACCTCAACATCTGAA+GGG 0.668709 tig0041758:+681 Msa1461820:intergenic
CAAACACCTCAGGCTCCGCA+CGG 0.675793 tig0041758:+326 Msa1461820:intergenic
GCGAGAAGAAGGAATTGTCG+TGG 0.678840 tig0041758:-784 Msa1461820:CDS
TAATGGCGGCAGCACCAGAG+TGG 0.679405 tig0041758:+898 Msa1461820:intergenic
TCAAAACCGACAGAAAACAA+AGG 0.688446 tig0041758:-829 Msa1461820:CDS
CATGTGACCAATGATAATGG+CGG 0.801428 tig0041758:+884 Msa1461810:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAACATAGAAAACAT+AGG + tig0041758:662-681 Msa1461820:intergenic 15.0%
!! AATAAAAACATAGAAAACAT+AGG + tig0041758:662-681 Msa1461820:intergenic 15.0%
!! TTATTTATGCTTTGATTATG+TGG - tig0041758:747-766 Msa1461820:CDS 20.0%
!! TTGAATGTTATGATTTCTTA+TGG - tig0041758:901-920 Msa1461820:CDS 20.0%
!! TGAATGTTATGATTTCTTAT+GGG - tig0041758:902-921 Msa1461820:CDS 20.0%
!! TAAACAGAAGAATTTCAATA+AGG - tig0041758:1028-1047 Msa1461820:five_prime_UTR 20.0%
!! TTATTTATGCTTTGATTATG+TGG - tig0041758:747-766 Msa1461820:CDS 20.0%
!! TTGAATGTTATGATTTCTTA+TGG - tig0041758:901-920 Msa1461820:CDS 20.0%
!! TGAATGTTATGATTTCTTAT+GGG - tig0041758:902-921 Msa1461820:CDS 20.0%
!! TAAACAGAAGAATTTCAATA+AGG - tig0041758:1028-1047 Msa1461820:five_prime_UTR 20.0%
! CATAAGGTAGTAAAATCTAA+TGG + tig0041758:381-400 Msa1461820:intergenic 25.0%
! ACATAGAAAACATAGGAAAA+TGG + tig0041758:655-674 Msa1461820:intergenic 25.0%
!! TTAGTATTTGTCTTTTACAC+TGG - tig0041758:715-734 Msa1461820:CDS 25.0%
! CATAAGGTAGTAAAATCTAA+TGG + tig0041758:381-400 Msa1461820:intergenic 25.0%
! ACATAGAAAACATAGGAAAA+TGG + tig0041758:655-674 Msa1461820:intergenic 25.0%
!! TTAGTATTTGTCTTTTACAC+TGG - tig0041758:715-734 Msa1461820:CDS 25.0%
!! GATAAATCCTGATTTTGAGA+CGG - tig0041758:282-301 Msa1461820:CDS 30.0%
TAGTAAAATCTAATGGAGTG+TGG + tig0041758:374-393 Msa1461820:intergenic 30.0%
GTAAAAATTTCTTCAGGAGA+TGG + tig0041758:611-630 Msa1461820:intergenic 30.0%
AGAAAATGTTCCATTTACTC+TGG - tig0041758:845-864 Msa1461820:CDS 30.0%
!! CATTCAAAACTTTAGAGTGA+TGG + tig0041758:888-907 Msa1461820:intergenic 30.0%
ACAGAAGAATTTCAATAAGG+AGG - tig0041758:1031-1050 Msa1461820:five_prime_UTR 30.0%
!! GATAAATCCTGATTTTGAGA+CGG - tig0041758:282-301 Msa1461820:CDS 30.0%
TAGTAAAATCTAATGGAGTG+TGG + tig0041758:374-393 Msa1461820:intergenic 30.0%
GTAAAAATTTCTTCAGGAGA+TGG + tig0041758:611-630 Msa1461820:intergenic 30.0%
AGAAAATGTTCCATTTACTC+TGG - tig0041758:845-864 Msa1461820:CDS 30.0%
!! CATTCAAAACTTTAGAGTGA+TGG + tig0041758:888-907 Msa1461820:intergenic 30.0%
ACAGAAGAATTTCAATAAGG+AGG - tig0041758:1031-1050 Msa1461820:five_prime_UTR 30.0%
AAAATCTAATGGAGTGTGGA+AGG + tig0041758:370-389 Msa1461820:intergenic 35.0%
GAACATGTGACCAATGATAA+TGG + tig0041758:437-456 Msa1461820:intergenic 35.0%
TCAAAACCGACAGAAAACAA+AGG - tig0041758:486-505 Msa1461820:CDS 35.0%
!!! GTTTTGCCTTTGTTTTCTGT+CGG + tig0041758:495-514 Msa1461820:intergenic 35.0%
AAACAAAGGCAAAACAATGC+AGG - tig0041758:500-519 Msa1461820:CDS 35.0%
!! GCTTGTTGTAAGCTTCAAAA+AGG - tig0041758:575-594 Msa1461820:intron 35.0%
ATGCTTTGATTATGTGGCAA+TGG - tig0041758:753-772 Msa1461820:CDS 35.0%
CAGCTCTACAATAGCTTAAT+AGG - tig0041758:785-804 Msa1461820:CDS 35.0%
AATAGCTTAATAGGACCAGA+TGG - tig0041758:794-813 Msa1461820:CDS 35.0%
TAATGTATACACCATCCATC+TGG + tig0041758:812-831 Msa1461820:intergenic 35.0%
!! AAAACTTTAGAGTGATGGTG+TGG + tig0041758:883-902 Msa1461820:intergenic 35.0%
! TCTTATGGGAATGTTAGCTT+TGG - tig0041758:916-935 Msa1461820:CDS 35.0%
AACAAAGATTCAAACACCTC+AGG + tig0041758:1002-1021 Msa1461820:intergenic 35.0%
! GAGGTGTTTGAATCTTTGTT+TGG - tig0041758:1002-1021 Msa1461820:five_prime_UTR 35.0%
AAAATCTAATGGAGTGTGGA+AGG + tig0041758:370-389 Msa1461820:intergenic 35.0%
GAACATGTGACCAATGATAA+TGG + tig0041758:437-456 Msa1461820:intergenic 35.0%
TCAAAACCGACAGAAAACAA+AGG - tig0041758:486-505 Msa1461820:CDS 35.0%
!!! GTTTTGCCTTTGTTTTCTGT+CGG + tig0041758:495-514 Msa1461820:intergenic 35.0%
AAACAAAGGCAAAACAATGC+AGG - tig0041758:500-519 Msa1461820:CDS 35.0%
!! GCTTGTTGTAAGCTTCAAAA+AGG - tig0041758:575-594 Msa1461820:intron 35.0%
ATGCTTTGATTATGTGGCAA+TGG - tig0041758:753-772 Msa1461820:CDS 35.0%
CAGCTCTACAATAGCTTAAT+AGG - tig0041758:785-804 Msa1461820:CDS 35.0%
AATAGCTTAATAGGACCAGA+TGG - tig0041758:794-813 Msa1461820:CDS 35.0%
TAATGTATACACCATCCATC+TGG + tig0041758:812-831 Msa1461820:intergenic 35.0%
!! AAAACTTTAGAGTGATGGTG+TGG + tig0041758:883-902 Msa1461820:intergenic 35.0%
! TCTTATGGGAATGTTAGCTT+TGG - tig0041758:916-935 Msa1461820:CDS 35.0%
AACAAAGATTCAAACACCTC+AGG + tig0041758:1002-1021 Msa1461820:intergenic 35.0%
! GAGGTGTTTGAATCTTTGTT+TGG - tig0041758:1002-1021 Msa1461820:five_prime_UTR 35.0%
!!! AAATCCTGATTTTGAGACGG+TGG - tig0041758:285-304 Msa1461820:CDS 40.0%
! TTCTTCTTTGCCTTGATAGC+AGG + tig0041758:314-333 Msa1461820:intergenic 40.0%
! AGTGTGGAAGGTTTTTGTAG+AGG + tig0041758:358-377 Msa1461820:intergenic 40.0%
!! CATGTGACCAATGATAATGG+CGG + tig0041758:434-453 Msa1461820:intergenic 40.0%
ATCATTGGTCACATGTTCAG+CGG - tig0041758:439-458 Msa1461820:intron 40.0%
!! GTTTTGACCGTAGTTGTATG+TGG + tig0041758:473-492 Msa1461820:intergenic 40.0%
CAAAACAATGCAGGTTTCAG+CGG - tig0041758:509-528 Msa1461820:CDS 40.0%
TCTTCAGGAGATGGATAAGA+AGG + tig0041758:602-621 Msa1461820:intergenic 40.0%
GGGTCGGTAAAAATTTCTTC+AGG + tig0041758:617-636 Msa1461820:intergenic 40.0%
AATGGACCTCAACATCTGAA+GGG + tig0041758:637-656 Msa1461820:intergenic 40.0%
AAATGGACCTCAACATCTGA+AGG + tig0041758:638-657 Msa1461820:intergenic 40.0%
! ATTTCAATAAGGAGGCATGC+AGG - tig0041758:1039-1058 Msa1461820:five_prime_UTR 40.0%
! TTTCAATAAGGAGGCATGCA+GGG - tig0041758:1040-1059 Msa1461820:five_prime_UTR 40.0%
!!! AAATCCTGATTTTGAGACGG+TGG - tig0041758:285-304 Msa1461820:CDS 40.0%
! TTCTTCTTTGCCTTGATAGC+AGG + tig0041758:314-333 Msa1461820:intergenic 40.0%
! AGTGTGGAAGGTTTTTGTAG+AGG + tig0041758:358-377 Msa1461820:intergenic 40.0%
!! CATGTGACCAATGATAATGG+CGG + tig0041758:434-453 Msa1461820:intergenic 40.0%
ATCATTGGTCACATGTTCAG+CGG - tig0041758:439-458 Msa1461820:intron 40.0%
!! GTTTTGACCGTAGTTGTATG+TGG + tig0041758:473-492 Msa1461820:intergenic 40.0%
CAAAACAATGCAGGTTTCAG+CGG - tig0041758:509-528 Msa1461820:CDS 40.0%
TCTTCAGGAGATGGATAAGA+AGG + tig0041758:602-621 Msa1461820:intergenic 40.0%
GGGTCGGTAAAAATTTCTTC+AGG + tig0041758:617-636 Msa1461820:intergenic 40.0%
AATGGACCTCAACATCTGAA+GGG + tig0041758:637-656 Msa1461820:intergenic 40.0%
AAATGGACCTCAACATCTGA+AGG + tig0041758:638-657 Msa1461820:intergenic 40.0%
GTTTCCACCGTCTCAAAATC+AGG + tig0041758:292-311 Msa1461820:intergenic 45.0%
CGGTGGTGTGAGATATCATA+AGG + tig0041758:397-416 Msa1461820:intergenic 45.0%
GCTTAATAGGACCAGATGGA+TGG - tig0041758:798-817 Msa1461820:CDS 45.0%
! TTAGAGTGATGGTGTGGTGT+GGG + tig0041758:877-896 Msa1461820:intergenic 45.0%
! TTTAGAGTGATGGTGTGGTG+TGG + tig0041758:878-897 Msa1461820:intergenic 45.0%
! TTCAATAAGGAGGCATGCAG+GGG - tig0041758:1041-1060 Msa1461820:five_prime_UTR 45.0%
GTTTCCACCGTCTCAAAATC+AGG + tig0041758:292-311 Msa1461820:intergenic 45.0%
CGGTGGTGTGAGATATCATA+AGG + tig0041758:397-416 Msa1461820:intergenic 45.0%
GCTTAATAGGACCAGATGGA+TGG - tig0041758:798-817 Msa1461820:CDS 45.0%
! TTAGAGTGATGGTGTGGTGT+GGG + tig0041758:877-896 Msa1461820:intergenic 45.0%
! TTTAGAGTGATGGTGTGGTG+TGG + tig0041758:878-897 Msa1461820:intergenic 45.0%
ACGGTGGAAACCTGCTATCA+AGG - tig0041758:301-320 Msa1461820:CDS 50.0%
GCGTCGTCCACATACAACTA+CGG - tig0041758:463-482 Msa1461820:intron 50.0%
GCGAGAAGAAGGAATTGTCG+TGG - tig0041758:531-550 Msa1461820:intron 50.0%
TACCGACCCTTCAGATGTTG+AGG - tig0041758:628-647 Msa1461820:intron 50.0%
GACCTCAACATCTGAAGGGT+CGG + tig0041758:633-652 Msa1461820:intergenic 50.0%
!! TGGGCACTGACCAGAGTAAA+TGG + tig0041758:858-877 Msa1461820:intergenic 50.0%
ACGGTGGAAACCTGCTATCA+AGG - tig0041758:301-320 Msa1461820:CDS 50.0%
GCGTCGTCCACATACAACTA+CGG - tig0041758:463-482 Msa1461820:intron 50.0%
GCGAGAAGAAGGAATTGTCG+TGG - tig0041758:531-550 Msa1461820:intron 50.0%
TACCGACCCTTCAGATGTTG+AGG - tig0041758:628-647 Msa1461820:intron 50.0%
GACCTCAACATCTGAAGGGT+CGG + tig0041758:633-652 Msa1461820:intergenic 50.0%
!! TGGGCACTGACCAGAGTAAA+TGG + tig0041758:858-877 Msa1461820:intergenic 50.0%
! GGTGCTGCCGCCATTATCAT+TGG - tig0041758:424-443 Msa1461820:intron 55.0%
AGGTTTCAGCGGCGAGAAGA+AGG - tig0041758:520-539 Msa1461820:CDS 55.0%
TTGTCGTGGCATTGCGTGCA+AGG - tig0041758:545-564 Msa1461820:intron 55.0%
TGGCATTGCGTGCAAGGTTG+TGG - tig0041758:551-570 Msa1461820:intron 55.0%
!! GCGTCCGTATTTTTGCCGTG+CGG - tig0041758:974-993 Msa1461820:five_prime_UTR 55.0%
GGCTCCGCACGGCAAAAATA+CGG + tig0041758:981-1000 Msa1461820:intergenic 55.0%
! GGTGCTGCCGCCATTATCAT+TGG - tig0041758:424-443 Msa1461820:intron 55.0%
AGGTTTCAGCGGCGAGAAGA+AGG - tig0041758:520-539 Msa1461820:CDS 55.0%
TTGTCGTGGCATTGCGTGCA+AGG - tig0041758:545-564 Msa1461820:intron 55.0%
TGGCATTGCGTGCAAGGTTG+TGG - tig0041758:551-570 Msa1461820:intron 55.0%
!! GCGTCCGTATTTTTGCCGTG+CGG - tig0041758:974-993 Msa1461820:five_prime_UTR 55.0%
GGCTCCGCACGGCAAAAATA+CGG + tig0041758:981-1000 Msa1461820:intergenic 55.0%
ATCTCACACCACCGCCACTC+TGG - tig0041758:403-422 Msa1461820:intron 60.0%
TAATGGCGGCAGCACCAGAG+TGG + tig0041758:420-439 Msa1461820:intergenic 60.0%
! TTTTTGCCGTGCGGAGCCTG+AGG - tig0041758:983-1002 Msa1461820:five_prime_UTR 60.0%
CAAACACCTCAGGCTCCGCA+CGG + tig0041758:992-1011 Msa1461820:intergenic 60.0%
ATCTCACACCACCGCCACTC+TGG - tig0041758:403-422 Msa1461820:intron 60.0%
TAATGGCGGCAGCACCAGAG+TGG + tig0041758:420-439 Msa1461820:intergenic 60.0%
! TTTTTGCCGTGCGGAGCCTG+AGG - tig0041758:983-1002 Msa1461820:five_prime_UTR 60.0%
CAAACACCTCAGGCTCCGCA+CGG + tig0041758:992-1011 Msa1461820:intergenic 60.0%
TGGCGGCAGCACCAGAGTGG+CGG + tig0041758:417-436 Msa1461820:intergenic 70.0%
TGGCGGCAGCACCAGAGTGG+CGG + tig0041758:417-436 Msa1461820:intergenic 70.0%
CGGCAGCACCAGAGTGGCGG+TGG + tig0041758:414-433 Msa1461820:intergenic 75.0%
CGGCAGCACCAGAGTGGCGG+TGG + tig0041758:414-433 Msa1461820:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0041758 gene 276 1061 276 ID=Msa1461820;Name=Msa1461820
tig0041758 mRNA 276 1061 276 ID=Msa1461820-mRNA-1;Parent=Msa1461820;Name=Msa1461820-mRNA-1;_AED=0.11;_eAED=0.11;_QI=122|1|0.66|1|1|1|3|0|140
tig0041758 exon 276 364 276 ID=Msa1461820-mRNA-1:exon:23370;Parent=Msa1461820-mRNA-1
tig0041758 exon 471 549 471 ID=Msa1461820-mRNA-1:exon:23369;Parent=Msa1461820-mRNA-1
tig0041758 exon 688 1061 688 ID=Msa1461820-mRNA-1:exon:23368;Parent=Msa1461820-mRNA-1
tig0041758 five_prime_UTR 940 1061 940 ID=Msa1461820-mRNA-1:five_prime_utr;Parent=Msa1461820-mRNA-1
tig0041758 CDS 688 939 688 ID=Msa1461820-mRNA-1:cds;Parent=Msa1461820-mRNA-1
tig0041758 CDS 471 549 471 ID=Msa1461820-mRNA-1:cds;Parent=Msa1461820-mRNA-1
tig0041758 CDS 276 364 276 ID=Msa1461820-mRNA-1:cds;Parent=Msa1461820-mRNA-1
Gene Sequence

>Msa1461820

ATGATATCTCACACCACCGCCACTCTGGTGCTGCCGCCATTATCATTGGTCACATGTTCAGCGGCGCGTCGTCCACATACAACTACGGTCAAAACCGACAGAAAACAAAGGCAAAACAATGCAGGTTTCAGCGGCGAGAAGAAGGAATTGTCGTGGCATTGCGTGCAAGGTTGTGGTGCTTGTTGTAAGCTTCAAAAAGGTCCTTCTTATCCATCTCCTGAAGAAATTTTTACCGACCCTTCAGATGTTGAGCTCTACAATAGCTTAATAGGACCAGATGGATGGTGTATACATTATGAAAAGAGTAGCAGAAAATGTTCCATTTACTCTGAGCGTCCGTATTTTTGCCGTGCGGAGCCTGAGGTGTTTGAATCTTTGTTTGGAGTTAAACAGAAGAATTTCAATAAGGAGGCATGCAGG

Protein sequence

>Msa1461820

MISHTTATLVLPPLSLVTCSAARRPHTTTVKTDRKQRQNNAGFSGEKKELSWHCVQGCGACCKLQKGPSYPSPEEIFTDPSDVELYNSLIGPDGWCIHYEKSSRKCSIYSERPYFCRAEPEVFESLFGVKQKNFNKEACR