AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATTTAACCTTGGTATT+TGG 0.131923 1.3:+5368107 None:intergenic
TACAAGGTTCTAGTATTAAT+TGG 0.175164 1.3:-5368170 MS.gene27790:CDS
CTTGATCATTGAAGCCTTGT+TGG 0.313961 1.3:+5368547 None:intergenic
AACCACCATGTTTGAGATTA+GGG 0.322373 1.3:+5369203 None:intergenic
CAACCATCAAAAGTAGTATT+AGG 0.326180 1.3:+5368468 None:intergenic
CTTGTTGCTGCTGATCTTTA+CGG 0.335855 1.3:+5368751 None:intergenic
ACAAGGTTCTAGTATTAATT+GGG 0.336603 1.3:-5368169 MS.gene27790:CDS
AAACCACCATGTTTGAGATT+AGG 0.366318 1.3:+5369202 None:intergenic
AATTATCCACCAAGATTTGA+TGG 0.368986 1.3:-5368243 MS.gene27790:CDS
CATTCCAGAGATTGATCATA+TGG 0.369915 1.3:-5368274 MS.gene27790:CDS
ATAATGACATAAAGAACTAT+TGG 0.370430 1.3:-5368809 MS.gene27790:CDS
TTGGTTGTGGTTGTTATCAT+TGG 0.385791 1.3:+5368356 None:intergenic
GATAATGTTATCTTCTTCTT+CGG 0.391795 1.3:+5369177 None:intergenic
ATGTCATTATCAGTTCTTCC+TGG 0.397545 1.3:+5368822 None:intergenic
TTCTTGAACCAAATTTGATA+AGG 0.407035 1.3:+5368650 None:intergenic
TGCATGACATGTGGTAAATA+TGG 0.407502 1.3:+5368624 None:intergenic
AGAAAACTTCTTATGAAGCT+TGG 0.411643 1.3:-5368516 MS.gene27790:CDS
TGCAGCCTTTACATTAGTAT+TGG 0.421799 1.3:-5369154 MS.gene27790:CDS
GCTCGTAATAGAGGCAATAA+TGG 0.432154 1.3:-5368729 MS.gene27790:CDS
CAGCAACAAGCTCGTAATAG+AGG 0.461617 1.3:-5368738 MS.gene27790:CDS
AATTCCAACCCATCAAATCT+TGG 0.462721 1.3:+5368234 None:intergenic
TCCACCAAGATTTGATGGGT+TGG 0.464644 1.3:-5368238 MS.gene27790:CDS
CATTTGTTGCGAATTGTACT+TGG 0.466808 1.3:+5368337 None:intergenic
AACATTGATGGTAGTGGAGG+TGG 0.467582 1.3:+5368582 None:intergenic
CAAGTACAATTCGCAACAAA+TGG 0.468082 1.3:-5368336 MS.gene27790:CDS
TTGCGAATTGTACTTGGTTG+TGG 0.482944 1.3:+5368343 None:intergenic
AAACTTCTTATGAAGCTTGG+AGG 0.489488 1.3:-5368513 MS.gene27790:CDS
TTTACATTAGTATTGGAAGC+AGG 0.496580 1.3:-5369147 MS.gene27790:intron
GATACCATATGATCAATCTC+TGG 0.503391 1.3:+5368270 None:intergenic
TTTGTGTATGACAACATTGA+TGG 0.508144 1.3:+5368570 None:intergenic
TGCTTCCAATACTAATGTAA+AGG 0.512738 1.3:+5369149 None:intergenic
GTGGAAAGAGTTGTCGTCTC+CGG 0.522056 1.3:-5369243 MS.gene27790:CDS
CACAAATCACAATTTAACCT+TGG 0.527729 1.3:+5368100 None:intergenic
CATTGATGGTAGTGGAGGTG+GGG 0.533607 1.3:+5368584 None:intergenic
ACATTGATGGTAGTGGAGGT+GGG 0.534119 1.3:+5368583 None:intergenic
ATAATTGCAGCTCAATTGCC+AGG 0.552173 1.3:-5368840 MS.gene27790:CDS
ATTATCCACCAAGATTTGAT+GGG 0.556403 1.3:-5368242 MS.gene27790:CDS
TATGACAACATTGATGGTAG+TGG 0.558740 1.3:+5368576 None:intergenic
ATTGATGGTAGTGGAGGTGG+GGG 0.560501 1.3:+5368585 None:intergenic
GGCGAAGATAGTTTAGCCAC+CGG 0.569935 1.3:+5369224 None:intergenic
TAAAGAACTATTGGAATACA+AGG 0.580554 1.3:-5368800 MS.gene27790:CDS
AAGATGATGATAACTTGAAG+TGG 0.584448 1.3:+5368137 None:intergenic
CTTCTTGATTCCAAATACCA+AGG 0.584605 1.3:-5368117 MS.gene27790:CDS
TCCAACCCATCAAATCTTGG+TGG 0.591459 1.3:+5368237 None:intergenic
ATTGATAATAATAAGATACA+AGG 0.601954 1.3:-5368186 MS.gene27790:CDS
AGTTTATGATCAAGAACATG+TGG 0.602665 1.3:-5368403 MS.gene27790:CDS
GAAAGAGTTGTCGTCTCCGG+TGG 0.603936 1.3:-5369240 MS.gene27790:CDS
CTTCGCCCTAATCTCAAACA+TGG 0.609085 1.3:-5369208 MS.gene27790:CDS
GACTCGGTGCAAGAAGAGCA+AGG 0.613143 1.3:-5368303 MS.gene27790:CDS
CAAGGTTCTAGTATTAATTG+GGG 0.621220 1.3:-5368168 MS.gene27790:CDS
CGCCCTAATCTCAAACATGG+TGG 0.637157 1.3:-5369205 MS.gene27790:CDS
TTGTCATACACAAACCAACA+AGG 0.660089 1.3:-5368561 MS.gene27790:CDS
GTTTATGATCAAGAACATGT+GGG 0.665568 1.3:-5368402 MS.gene27790:CDS
AAATGGTCAATACTTTGACT+CGG 0.679619 1.3:-5368319 MS.gene27790:CDS
GACAACATTGATGGTAGTGG+AGG 0.681316 1.3:+5368579 None:intergenic
AGCAAAGGTTGCATGACATG+TGG 0.714384 1.3:+5368615 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAATTCAAACTAT+TGG + chr1.3:5368419-5368438 None:intergenic 10.0%
!!! ATTGATAATAATAAGATACA+AGG - chr1.3:5369182-5369201 MS.gene27790:CDS 15.0%
!! ACAAAAAGTAACATATCATA+TGG + chr1.3:5368386-5368405 None:intergenic 20.0%
!! ATAATGACATAAAGAACTAT+TGG - chr1.3:5368559-5368578 MS.gene27790:CDS 20.0%
!! ATCTATAAAATCAAGACATA+AGG + chr1.3:5368342-5368361 None:intergenic 20.0%
!!! TGAATTTTCCTTATCAAATT+TGG - chr1.3:5368710-5368729 MS.gene27790:CDS 20.0%
!!! TGATATGTTACTTTTTGTTT+TGG - chr1.3:5368388-5368407 MS.gene27790:CDS 20.0%
! GATAATGTTATCTTCTTCTT+CGG + chr1.3:5368194-5368213 None:intergenic 25.0%
! TAAAGAACTATTGGAATACA+AGG - chr1.3:5368568-5368587 MS.gene27790:CDS 25.0%
! TTCTTGAACCAAATTTGATA+AGG + chr1.3:5368721-5368740 None:intergenic 25.0%
!! ACAAGGTTCTAGTATTAATT+GGG - chr1.3:5369199-5369218 MS.gene27790:CDS 25.0%
!! TACAAGGTTCTAGTATTAAT+TGG - chr1.3:5369198-5369217 MS.gene27790:CDS 25.0%
AAATGGTCAATACTTTGACT+CGG - chr1.3:5369049-5369068 MS.gene27790:intron 30.0%
AAGATGATGATAACTTGAAG+TGG + chr1.3:5369234-5369253 None:intergenic 30.0%
AATTATCCACCAAGATTTGA+TGG - chr1.3:5369125-5369144 MS.gene27790:intron 30.0%
AGAAAACTTCTTATGAAGCT+TGG - chr1.3:5368852-5368871 MS.gene27790:intron 30.0%
AGTTTATGATCAAGAACATG+TGG - chr1.3:5368965-5368984 MS.gene27790:intron 30.0%
ATTATCCACCAAGATTTGAT+GGG - chr1.3:5369126-5369145 MS.gene27790:intron 30.0%
CAACCATCAAAAGTAGTATT+AGG + chr1.3:5368903-5368922 None:intergenic 30.0%
CTTTCATACCAAACCAAAAA+GGG + chr1.3:5368498-5368517 None:intergenic 30.0%
GTTTATGATCAAGAACATGT+GGG - chr1.3:5368966-5368985 MS.gene27790:intron 30.0%
TGCTTCCAATACTAATGTAA+AGG + chr1.3:5368222-5368241 None:intergenic 30.0%
TTTGTGTATGACAACATTGA+TGG + chr1.3:5368801-5368820 None:intergenic 30.0%
! AAGGTTGAAAAAGAAGCTTT+TGG - chr1.3:5368587-5368606 MS.gene27790:CDS 30.0%
! AGGTTGAAAAAGAAGCTTTT+GGG - chr1.3:5368588-5368607 MS.gene27790:CDS 30.0%
! CAAGGTTCTAGTATTAATTG+GGG - chr1.3:5369200-5369219 MS.gene27790:CDS 30.0%
! TTTACATTAGTATTGGAAGC+AGG - chr1.3:5368221-5368240 MS.gene27790:CDS 30.0%
!! CATCCTAATACTACTTTTGA+TGG - chr1.3:5368897-5368916 MS.gene27790:intron 30.0%
AAACTTCTTATGAAGCTTGG+AGG - chr1.3:5368855-5368874 MS.gene27790:intron 35.0%
AATTCCAACCCATCAAATCT+TGG + chr1.3:5369137-5369156 None:intergenic 35.0%
ATGTCATTATCAGTTCTTCC+TGG + chr1.3:5368549-5368568 None:intergenic 35.0%
CAAGTACAATTCGCAACAAA+TGG - chr1.3:5369032-5369051 MS.gene27790:intron 35.0%
CATTCCAGAGATTGATCATA+TGG - chr1.3:5369094-5369113 MS.gene27790:intron 35.0%
CTTCTTGATTCCAAATACCA+AGG - chr1.3:5369251-5369270 MS.gene27790:CDS 35.0%
GATACCATATGATCAATCTC+TGG + chr1.3:5369101-5369120 None:intergenic 35.0%
GCTTTCATACCAAACCAAAA+AGG + chr1.3:5368499-5368518 None:intergenic 35.0%
TGCAGCCTTTACATTAGTAT+TGG - chr1.3:5368214-5368233 MS.gene27790:CDS 35.0%
TGCATGACATGTGGTAAATA+TGG + chr1.3:5368747-5368766 None:intergenic 35.0%
TTGGTTGTGGTTGTTATCAT+TGG + chr1.3:5369015-5369034 None:intergenic 35.0%
TTGTCATACACAAACCAACA+AGG - chr1.3:5368807-5368826 MS.gene27790:CDS 35.0%
! AAACCACCATGTTTGAGATT+AGG + chr1.3:5368169-5368188 None:intergenic 35.0%
! AACCACCATGTTTGAGATTA+GGG + chr1.3:5368168-5368187 None:intergenic 35.0%
! CATTTGTTGCGAATTGTACT+TGG + chr1.3:5369034-5369053 None:intergenic 35.0%
! GGTTGAAAAAGAAGCTTTTG+GGG - chr1.3:5368589-5368608 MS.gene27790:CDS 35.0%
! TATGACAACATTGATGGTAG+TGG + chr1.3:5368795-5368814 None:intergenic 35.0%
! TGAAAAGTGAAGACCCTTTT+TGG - chr1.3:5368482-5368501 MS.gene27790:CDS 35.0%
!!! GATGGGTTGGAATTCTTTTA+TGG - chr1.3:5369143-5369162 MS.gene27790:intron 35.0%
!!! TTTTGGTTTGGTATGAAAGC+AGG - chr1.3:5368499-5368518 MS.gene27790:CDS 35.0%
ATAATTGCAGCTCAATTGCC+AGG - chr1.3:5368528-5368547 MS.gene27790:CDS 40.0%
CTTGTTGCTGCTGATCTTTA+CGG + chr1.3:5368620-5368639 None:intergenic 40.0%
GCTCGTAATAGAGGCAATAA+TGG - chr1.3:5368639-5368658 MS.gene27790:CDS 40.0%
TTGCGAATTGTACTTGGTTG+TGG + chr1.3:5369028-5369047 None:intergenic 40.0%
! AAGCTTGGAGGAAGATTTTC+AGG - chr1.3:5368867-5368886 MS.gene27790:intron 40.0%
! CTTGATCATTGAAGCCTTGT+TGG + chr1.3:5368824-5368843 None:intergenic 40.0%
!!! AGTGAAGACCCTTTTTGGTT+TGG - chr1.3:5368487-5368506 MS.gene27790:CDS 40.0%
AGCAAAGGTTGCATGACATG+TGG + chr1.3:5368756-5368775 None:intergenic 45.0%
CAGCAACAAGCTCGTAATAG+AGG - chr1.3:5368630-5368649 MS.gene27790:CDS 45.0%
CTTCGCCCTAATCTCAAACA+TGG - chr1.3:5368160-5368179 MS.gene27790:CDS 45.0%
TCCAACCCATCAAATCTTGG+TGG + chr1.3:5369134-5369153 None:intergenic 45.0%
! TCCACCAAGATTTGATGGGT+TGG - chr1.3:5369130-5369149 MS.gene27790:intron 45.0%
!! AACATTGATGGTAGTGGAGG+TGG + chr1.3:5368789-5368808 None:intergenic 45.0%
!! ACATTGATGGTAGTGGAGGT+GGG + chr1.3:5368788-5368807 None:intergenic 45.0%
!! GACAACATTGATGGTAGTGG+AGG + chr1.3:5368792-5368811 None:intergenic 45.0%
!! TGGTTTGGTATGAAAGCAGG+TGG - chr1.3:5368502-5368521 MS.gene27790:CDS 45.0%
CGCCCTAATCTCAAACATGG+TGG - chr1.3:5368163-5368182 MS.gene27790:CDS 50.0%
GGCGAAGATAGTTTAGCCAC+CGG + chr1.3:5368147-5368166 None:intergenic 50.0%
GTGGAAAGAGTTGTCGTCTC+CGG - chr1.3:5368125-5368144 MS.gene27790:CDS 50.0%
!! ATTGATGGTAGTGGAGGTGG+GGG + chr1.3:5368786-5368805 None:intergenic 50.0%
!! CATTGATGGTAGTGGAGGTG+GGG + chr1.3:5368787-5368806 None:intergenic 50.0%
GAAAGAGTTGTCGTCTCCGG+TGG - chr1.3:5368128-5368147 MS.gene27790:CDS 55.0%
!! GACTCGGTGCAAGAAGAGCA+AGG - chr1.3:5369065-5369084 MS.gene27790:intron 55.0%
!! GGTGGGGGTGTTGATAGCAA+AGG + chr1.3:5368771-5368790 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 5368113 5369277 5368113 ID=MS.gene27790
chr1.3 mRNA 5368113 5369277 5368113 ID=MS.gene27790.t1;Parent=MS.gene27790
chr1.3 exon 5369148 5369277 5369148 ID=MS.gene27790.t1.exon1;Parent=MS.gene27790.t1
chr1.3 CDS 5369148 5369277 5369148 ID=cds.MS.gene27790.t1;Parent=MS.gene27790.t1
chr1.3 exon 5368113 5368869 5368113 ID=MS.gene27790.t1.exon2;Parent=MS.gene27790.t1
chr1.3 CDS 5368113 5368869 5368113 ID=cds.MS.gene27790.t1;Parent=MS.gene27790.t1
Gene Sequence

>MS.gene27790

GCTTGAAGCGTTGTGGAAAGAGTTGTCGTCTCCGGTGGCTAAACTATCTTCGCCCTAATCTCAAACATGGTGGTTTTTCCGAAGAAGAAGATAACATTATCTGCAGCCTTTACATTAGTATTGGAAGCAGGTACATATACATATGCTTAGTTTCTTGCATAACATAATTGATCATTTGCAACTGCAATTCAAGCTGCATAGATTAAGAATCAAGATGTAATTGTAACCTTATGTCTTGATTTTATAGATACACTACAATTTATATTATAACCATATGATATGTTACTTTTTGTTTTGGTGCAACCAATAGTTTGAATTTTTTTTAACTATCTATATATATAATTATTATATATATAGTATATATAGATGTGAAAAGTGAAGACCCTTTTTGGTTTGGTATGAAAGCAGGTGGTCGATAATTGCAGCTCAATTGCCAGGAAGAACTGATAATGACATAAAGAACTATTGGAATACAAGGTTGAAAAAGAAGCTTTTGGGGAAACACCGTAAAGATCAGCAGCAACAAGCTCGTAATAGAGGCAATAATGGTGTTATTATTAAGCAAGAAAGCAATAACAATAGAGTGATGAGTAGTAGTGAATTTTCCTTATCAAATTTGGTTCAAGAACAACCATATTTACCACATGTCATGCAACCTTTGCTATCAACACCCCCACCTCCACTACCATCAATGTTGTCATACACAAACCAACAAGGCTTCAATGATCAAGATTCTATTAGAAAACTTCTTATGAAGCTTGGAGGAAGATTTTCAGGTGATTATCATCCTAATACTACTTTTGATGGTTGTAATCTTCAATTTTCAAATGCTACTTCTTCAACTCATGAACAAGTTTATGATCAAGAACATGTGGGTTCTTCTACTTGTGTTAATTCTTCCAATGATAACAACCACAACCAAGTACAATTCGCAACAAATGGTCAATACTTTGACTCGGTGCAAGAAGAGCAAGGTAAATTCATTCCAGAGATTGATCATATGGTATCTACTAATTATCCACCAAGATTTGATGGGTTGGAATTCTTTTATGGTGAAGAAGACTTGAATATTGATAATAATAAGATACAAGGTTCTAGTATTAATTGGGGTGAAGCTACCACTTCAAGTTATCATCATCTTCTTGATTCCAAATACCAAGGTTAA

Protein sequence

>MS.gene27790.t1

AUSVVERVVVSGGUTIFALISNMVVFPKKKITLSAAFTLVLEAGGRULQLNCQEELIMTURTIGIQGUKRSFWGNTVKISSNKLVIEAIMVLLLSKKAITIEUUVVVNFPYQIWFKNNHIYHMSCNLCYQHPHLHYHQCCHTQTNKASMIKILLENFLUSLEEDFQVIIILILLLMVVIFNFQMLLLQLMNKFMIKNMWVLLLVLILPMITTTTKYNSQQMVNTLTRCKKSKVNSFQRLIIWYLLIIHQDLMGWNSFMVKKTUILIIIRYKVLVLIGVKLPLQVIIIFLIPNTKV