AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61145


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61145.t1 MTR_4g097500 94.483 145 8 0 1 145 1 145 1.60e-100 288
MS.gene61145.t1 MTR_7g100540 29.801 151 94 4 2 145 4 149 2.91e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61145.t1 AT5G65840 68.000 100 29 1 49 145 70 169 2.51e-43 144
MS.gene61145.t1 AT2G37240 32.632 95 62 1 51 145 54 146 3.49e-11 59.3

Find 37 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGAAGGTATTAGATTT+TGG 0.154519 8.1:+25097784 None:intergenic
CTGTCATGTTCAAAGATCTT+TGG 0.216394 8.1:-25097642 MS.gene61145:CDS
AGGGGTAAGTGTGTGAATTC+GGG 0.243170 8.1:+25097827 None:intergenic
GAGGGGTAAGTGTGTGAATT+CGG 0.256228 8.1:+25097826 None:intergenic
AGCAAGAATACGAGCTTTAT+TGG 0.271846 8.1:+25096729 None:intergenic
GCAAGAATACGAGCTTTATT+GGG 0.301563 8.1:+25096730 None:intergenic
CAGAAAGAAGTCGTTTGTTT+CGG 0.314726 8.1:+25097746 None:intergenic
AGCTGCATCAAACCTTGATT+TGG 0.325737 8.1:+25096786 None:intergenic
TGTCATGTTCAAAGATCTTT+GGG 0.351307 8.1:-25097641 MS.gene61145:CDS
TGTTCTTCTTAGTTGGGAAC+TGG 0.353355 8.1:-25096827 MS.gene61145:intron
AGAAAGAAGTCGTTTGTTTC+GGG 0.362238 8.1:+25097747 None:intergenic
GTTCAAAGATCTTTGGGATC+AGG 0.419236 8.1:-25097635 MS.gene61145:CDS
AGATCTTTGGGATCAGGAAC+AGG 0.458429 8.1:-25097629 MS.gene61145:intron
CCCACTGTTGCAGAGAATCT+AGG 0.472919 8.1:-25097697 MS.gene61145:CDS
CATTGAAAGAATCCAAATCA+AGG 0.492034 8.1:-25096798 MS.gene61145:CDS
GCCGATGAATGAAGATTTGA+AGG 0.493342 8.1:+25097771 None:intergenic
TGGGGTGTAGTGAATGGTAG+AGG 0.500971 8.1:+25097804 None:intergenic
GGCACTTTGGATGCCCATGC+TGG 0.513878 8.1:-25096934 MS.gene61145:intron
ACCTTCAAATCTTCATTCAT+CGG 0.522192 8.1:-25097772 MS.gene61145:CDS
AAATCAAGGTTTGATGCAGC+TGG 0.524810 8.1:-25096784 MS.gene61145:CDS
AAGCTAATTGCAGTGGGCGT+TGG 0.548964 8.1:-25096757 MS.gene61145:CDS
GGGGTAAGTGTGTGAATTCG+GGG 0.552268 8.1:+25097828 None:intergenic
GGTGTGATAGATGGATGAAA+AGG 0.565786 8.1:+25097850 None:intergenic
AGATCATTAGTTACCAGCAT+GGG 0.566257 8.1:+25096921 None:intergenic
GCCTAGATTCTCTGCAACAG+TGG 0.569349 8.1:+25097696 None:intergenic
CAAGAATACGAGCTTTATTG+GGG 0.569412 8.1:+25096731 None:intergenic
GTGTAGTGAATGGTAGAGGA+GGG 0.571659 8.1:+25097808 None:intergenic
TGGTGTCAAGCTAATTGCAG+TGG 0.616794 8.1:-25096764 MS.gene61145:CDS
GGTGTAGTGAATGGTAGAGG+AGG 0.617740 8.1:+25097807 None:intergenic
CCTAGATTCTCTGCAACAGT+GGG 0.622553 8.1:+25097697 None:intergenic
ATTCTTACAGGGAATAGCTG+TGG 0.623503 8.1:-25096969 MS.gene61145:intron
AAGATCATTAGTTACCAGCA+TGG 0.626138 8.1:+25096920 None:intergenic
GGTGTCAAGCTAATTGCAGT+GGG 0.627555 8.1:-25096763 MS.gene61145:CDS
GGGTAAGTGTGTGAATTCGG+GGG 0.635427 8.1:+25097829 None:intergenic
CTTACAGGGAATAGCTGTGG+TGG 0.639248 8.1:-25096966 MS.gene61145:intron
TGTAGTGAATGGTAGAGGAG+GGG 0.653187 8.1:+25097809 None:intergenic
AAGAATACGAGCTTTATTGG+GGG 0.731789 8.1:+25096732 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATAAAATAAAGTTAAAT+TGG - chr8.1:25097435-25097454 MS.gene61145:intron 10.0%
!! ATTTATTATTTATCCAAGTA+AGG + chr8.1:25097358-25097377 None:intergenic 15.0%
!! GAATTGAAATTAATTTATTC+TGG - chr8.1:25097248-25097267 MS.gene61145:intron 15.0%
!!! TTTTACTCTTTAAATGTTTT+GGG - chr8.1:25097032-25097051 MS.gene61145:intron 15.0%
!!! TTTTTACTCTTTAAATGTTT+TGG - chr8.1:25097031-25097050 MS.gene61145:intron 15.0%
!! TACTAACTAGTTGAATTAAT+AGG - chr8.1:25097578-25097597 MS.gene61145:intron 20.0%
!!! AATTAGTTGAAATTGATTTG+AGG - chr8.1:25097163-25097182 MS.gene61145:intron 20.0%
!!! ATTTGAAGGTATTAGATTTT+GGG + chr8.1:25096821-25096840 None:intergenic 20.0%
!!! TAGTTAGTATCTATTTTGAT+TGG + chr8.1:25097567-25097586 None:intergenic 20.0%
!!! TAGTTGGAATTAATTCATTT+TGG - chr8.1:25097195-25097214 MS.gene61145:intron 20.0%
!!! TTTTTTTTTGACCTAAATTG+TGG - chr8.1:25097069-25097088 MS.gene61145:intron 20.0%
! GATTAGAGTTAATTCTTACA+GGG - chr8.1:25097623-25097642 MS.gene61145:intron 25.0%
! TAAAGCAAAAACCACAATTT+AGG + chr8.1:25097083-25097102 None:intergenic 25.0%
! TGATTAGAGTTAATTCTTAC+AGG - chr8.1:25097622-25097641 MS.gene61145:intron 25.0%
!! AAATTGTGGTTTTTGCTTTA+TGG - chr8.1:25097083-25097102 MS.gene61145:intron 25.0%
!! ATTAATAACCAAAAGCACAA+AGG + chr8.1:25097711-25097730 None:intergenic 25.0%
!!! AATAGGTTGATGTTAATATG+TGG - chr8.1:25097595-25097614 MS.gene61145:intron 25.0%
!!! GATTTGAAGGTATTAGATTT+TGG + chr8.1:25096822-25096841 None:intergenic 25.0%
!!! TTGTGCTTTTGGTTATTAAT+CGG - chr8.1:25097711-25097730 MS.gene61145:CDS 25.0%
!!! TTTGAAGGTATTAGATTTTG+GGG + chr8.1:25096820-25096839 None:intergenic 25.0%
!!! TTTGAGGTTGATATATTAGT+TGG - chr8.1:25097179-25097198 MS.gene61145:intron 25.0%
ACCTTCAAATCTTCATTCAT+CGG - chr8.1:25096831-25096850 MS.gene61145:intron 30.0%
CATTGAAAGAATCCAAATCA+AGG - chr8.1:25097805-25097824 MS.gene61145:CDS 30.0%
TCAACACAACATTAACAAAC+AGG + chr8.1:25097130-25097149 None:intergenic 30.0%
TGTCATGTTCAAAGATCTTT+GGG - chr8.1:25096962-25096981 MS.gene61145:CDS 30.0%
! AATAAGTTGAAACCAGCTTA+TGG - chr8.1:25097470-25097489 MS.gene61145:intron 30.0%
! TTTGAAGTTGTATCAGATCA+AGG - chr8.1:25097332-25097351 MS.gene61145:intron 30.0%
!! ATAAGTTTTGTGAGGCTATT+TGG + chr8.1:25097521-25097540 None:intergenic 30.0%
!! TAAGTTTTGTGAGGCTATTT+GGG + chr8.1:25097520-25097539 None:intergenic 30.0%
!! TGTGCTTTTGGTTATTAATC+GGG - chr8.1:25097712-25097731 MS.gene61145:CDS 30.0%
AAGATCATTAGTTACCAGCA+TGG + chr8.1:25097686-25097705 None:intergenic 35.0%
ACAACATTAACAAACAGGAG+GGG + chr8.1:25097125-25097144 None:intergenic 35.0%
ACACAACATTAACAAACAGG+AGG + chr8.1:25097127-25097146 None:intergenic 35.0%
ACTGATAGTTGTTCTTACTG+TGG - chr8.1:25097744-25097763 MS.gene61145:CDS 35.0%
AGAAAGAAGTCGTTTGTTTC+GGG + chr8.1:25096859-25096878 None:intergenic 35.0%
AGATCATTAGTTACCAGCAT+GGG + chr8.1:25097685-25097704 None:intergenic 35.0%
CACAACATTAACAAACAGGA+GGG + chr8.1:25097126-25097145 None:intergenic 35.0%
CAGAAAGAAGTCGTTTGTTT+CGG + chr8.1:25096860-25096879 None:intergenic 35.0%
CTGTCATGTTCAAAGATCTT+TGG - chr8.1:25096961-25096980 MS.gene61145:CDS 35.0%
GTCATACTTTACTTACCTAC+AGG + chr8.1:25097292-25097311 None:intergenic 35.0%
GTCTTCTGTTCTTCTTAGTT+GGG - chr8.1:25097770-25097789 MS.gene61145:CDS 35.0%
TGTCTTCTGTTCTTCTTAGT+TGG - chr8.1:25097769-25097788 MS.gene61145:CDS 35.0%
! AGCAAGAATACGAGCTTTAT+TGG + chr8.1:25097877-25097896 None:intergenic 35.0%
! GGTTGATGTTAATATGTGGT+TGG - chr8.1:25097599-25097618 MS.gene61145:intron 35.0%
! TTAGTATTTTCACTGCTGCT+GGG - chr8.1:25096934-25096953 MS.gene61145:intron 35.0%
!! AAGAATACGAGCTTTATTGG+GGG + chr8.1:25097874-25097893 None:intergenic 35.0%
!! CAAGAATACGAGCTTTATTG+GGG + chr8.1:25097875-25097894 None:intergenic 35.0%
!! CTACTGACATAAGTTTTGTG+AGG + chr8.1:25097529-25097548 None:intergenic 35.0%
!! GCAAGAATACGAGCTTTATT+GGG + chr8.1:25097876-25097895 None:intergenic 35.0%
AAATCAAGGTTTGATGCAGC+TGG - chr8.1:25097819-25097838 MS.gene61145:CDS 40.0%
AACAGACATTGTCCATAAGC+TGG + chr8.1:25097485-25097504 None:intergenic 40.0%
AGCTGCATCAAACCTTGATT+TGG + chr8.1:25097820-25097839 None:intergenic 40.0%
ATTCTTACAGGGAATAGCTG+TGG - chr8.1:25097634-25097653 MS.gene61145:CDS 40.0%
GCCGATGAATGAAGATTTGA+AGG + chr8.1:25096835-25096854 None:intergenic 40.0%
GGTGTGATAGATGGATGAAA+AGG + chr8.1:25096756-25096775 None:intergenic 40.0%
TATCAGATCAAGGCCTTACT+TGG - chr8.1:25097342-25097361 MS.gene61145:intron 40.0%
TGTTCTTCTTAGTTGGGAAC+TGG - chr8.1:25097776-25097795 MS.gene61145:CDS 40.0%
! GATCTTTGCCTTTGTGCTTT+TGG - chr8.1:25097700-25097719 MS.gene61145:CDS 40.0%
! GTTAGTATTTTCACTGCTGC+TGG - chr8.1:25096933-25096952 MS.gene61145:intron 40.0%
! TAGTATTTTCACTGCTGCTG+GGG - chr8.1:25096935-25096954 MS.gene61145:CDS 40.0%
! TCTGGTGTTAACCTAGTGTT+TGG - chr8.1:25097266-25097285 MS.gene61145:intron 40.0%
!! GTTCAAAGATCTTTGGGATC+AGG - chr8.1:25096968-25096987 MS.gene61145:intron 40.0%
!!! AGATTTTGGGGTGTAGTGAA+TGG + chr8.1:25096808-25096827 None:intergenic 40.0%
AGGGGTAAGTGTGTGAATTC+GGG + chr8.1:25096779-25096798 None:intergenic 45.0%
CCTAGATTCTCTGCAACAGT+GGG + chr8.1:25096909-25096928 None:intergenic 45.0%
GAGGGGTAAGTGTGTGAATT+CGG + chr8.1:25096780-25096799 None:intergenic 45.0%
GGTGTCAAGCTAATTGCAGT+GGG - chr8.1:25097840-25097859 MS.gene61145:CDS 45.0%
GTGTAGTGAATGGTAGAGGA+GGG + chr8.1:25096798-25096817 None:intergenic 45.0%
TAACCTAGTGTTTGGCCTGT+AGG - chr8.1:25097274-25097293 MS.gene61145:intron 45.0%
TGTAGTGAATGGTAGAGGAG+GGG + chr8.1:25096797-25096816 None:intergenic 45.0%
TTACCTACAGGCCAAACACT+AGG + chr8.1:25097280-25097299 None:intergenic 45.0%
! TAGCTGTGGTGGCACTTTTA+AGG - chr8.1:25097648-25097667 MS.gene61145:CDS 45.0%
! TGGTGTCAAGCTAATTGCAG+TGG - chr8.1:25097839-25097858 MS.gene61145:CDS 45.0%
!! AGATCTTTGGGATCAGGAAC+AGG - chr8.1:25096974-25096993 MS.gene61145:intron 45.0%
!!! GTGGCACTTTTAAGGCACTT+TGG - chr8.1:25097656-25097675 MS.gene61145:CDS 45.0%
AAGCTAATTGCAGTGGGCGT+TGG - chr8.1:25097846-25097865 MS.gene61145:CDS 50.0%
CCCACTGTTGCAGAGAATCT+AGG - chr8.1:25096906-25096925 MS.gene61145:intron 50.0%
CTTACAGGGAATAGCTGTGG+TGG - chr8.1:25097637-25097656 MS.gene61145:CDS 50.0%
GCCTAGATTCTCTGCAACAG+TGG + chr8.1:25096910-25096929 None:intergenic 50.0%
GGATCAGGAACAGGTCAGAA+TGG - chr8.1:25096983-25097002 MS.gene61145:intron 50.0%
GGGGTAAGTGTGTGAATTCG+GGG + chr8.1:25096778-25096797 None:intergenic 50.0%
GGGTAAGTGTGTGAATTCGG+GGG + chr8.1:25096777-25096796 None:intergenic 50.0%
GGTGTAGTGAATGGTAGAGG+AGG + chr8.1:25096799-25096818 None:intergenic 50.0%
TGGGGTGTAGTGAATGGTAG+AGG + chr8.1:25096802-25096821 None:intergenic 50.0%
! GAATTCGGGGGTGTGATAGA+TGG + chr8.1:25096765-25096784 None:intergenic 50.0%
GGCACTTTGGATGCCCATGC+TGG - chr8.1:25097669-25097688 MS.gene61145:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25096723 25097902 25096723 ID=MS.gene61145
chr8.1 mRNA 25096723 25097902 25096723 ID=MS.gene61145.t1;Parent=MS.gene61145
chr8.1 exon 25097630 25097902 25097630 ID=MS.gene61145.t1.exon1;Parent=MS.gene61145.t1
chr8.1 CDS 25097630 25097902 25097630 ID=cds.MS.gene61145.t1;Parent=MS.gene61145.t1
chr8.1 exon 25096935 25096981 25096935 ID=MS.gene61145.t1.exon2;Parent=MS.gene61145.t1
chr8.1 CDS 25096935 25096981 25096935 ID=cds.MS.gene61145.t1;Parent=MS.gene61145.t1
chr8.1 exon 25096723 25096837 25096723 ID=MS.gene61145.t1.exon3;Parent=MS.gene61145.t1
chr8.1 CDS 25096723 25096837 25096723 ID=cds.MS.gene61145.t1;Parent=MS.gene61145.t1
Gene Sequence

>MS.gene61145

ATGGCAACAACTCTACAATCTCTTCGATTTCCTTTTCATCCATCTATCACACCCCCGAATTCACACACTTACCCCTCCTCTACCATTCACTACACCCCAAAATCTAATACCTTCAAATCTTCATTCATCGGCACCCGAAACAAACGACTTCTTTCTGCTAGAGCTTCGAGTTCACAGTATAGTCCCACTGTTGCAGAGAATCTAGGCGATGTTAGTATTTTCACTGCTGCTGGGGAATCTGTCATGTTCAAAGATCTTTGGGATCAGGAACAGGTCAGAATGGTTATGTTCGTATAAAAAAGTTTTGATTTTTACTCTTTAAATGTTTTGGGCACATCACAATTTTTTTTTTTTTGACCTAAATTGTGGTTTTTGCTTTATGGAATACTAGATTAATCACCCCTCCTGTTTGTTAATGTTGTGTTGAAGTAGTATGAGTTAATTAGTTGAAATTGATTTGAGGTTGATATATTAGTTGGAATTAATTCATTTTGGTCGTAAGTAAAGTCTGACTTAGAATTAGTTGAATTGAAATTAATTTATTCTGGTGTTAACCTAGTGTTTGGCCTGTAGGTAAGTAAAGTATGACTTAGAATTAGTTTAAATTAATTTGAAGTTGTATCAGATCAAGGCCTTACTTGGATAAATAATAAATTACTTATTTGCAGCTTATCACGATATGTGCGTATATATAAGCTATAAGCTATAGCAAAAGATAAAATAAAGTTAAATTGGAGAACTTGTGAAAATAAGTTGAAACCAGCTTATGGACAATGTCTGTTTTCATAAGTTATCCCAAATAGCCTCACAAAACTTATGTCAGTAGATAAACTCAAATTAGCCAATCAAAATAGATACTAACTAGTTGAATTAATAGGTTGATGTTAATATGTGGTTGGTGATTAGAGTTAATTCTTACAGGGAATAGCTGTGGTGGCACTTTTAAGGCACTTTGGATGCCCATGCTGGTAACTAATGATCTTTGCCTTTGTGCTTTTGGTTATTAATCGGGATTTGTTTGACTGATAGTTGTTCTTACTGTGGATTGTCTTCTGTTCTTCTTAGTTGGGAACTGGCTTCAACATTGAAAGAATCCAAATCAAGGTTTGATGCAGCTGGTGTCAAGCTAATTGCAGTGGGCGTTGGTGCCCCCAATAAAGCTCGTATTCTTGCTGAACGA

Protein sequence

>MS.gene61145.t1

MATTLQSLRFPFHPSITPPNSHTYPSSTIHYTPKSNTFKSSFIGTRNKRLLSARASSSQYSPTVAENLGDVSIFTAAGESVMFKDLWDQEQGIAVVALLRHFGCPCCWELASTLKESKSRFDAAGVKLIAVGVGAPNKARILAER