AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44440.t1 MTR_4g097500 91.209 91 8 0 1 91 1 91 3.51e-57 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGAAGGTATTAGATTT+TGG 0.154519 8.1:+25111485 None:intergenic
CTGTCATGTTCAAAGATCTT+TGG 0.216394 8.1:-25111343 MS.gene44440:CDS
AGGGGTAAGTGTGTGAATTC+GGG 0.243170 8.1:+25111528 None:intergenic
GAGGGGTAAGTGTGTGAATT+CGG 0.256228 8.1:+25111527 None:intergenic
CAGAAAGAAGTCGTTTGTTT+CGG 0.314726 8.1:+25111447 None:intergenic
TGTCATGTTCAAAGATCTTT+GGG 0.351307 8.1:-25111342 MS.gene44440:CDS
AGAAAGAAGTCGTTTGTTTC+GGG 0.362238 8.1:+25111448 None:intergenic
GTTCAAAGATCTTTGGGATC+AGG 0.419236 8.1:-25111336 MS.gene44440:CDS
AGATCTTTGGGATCAGGAAC+AGG 0.458429 8.1:-25111330 None:intergenic
CCCACTGTTGCAGAGAATCT+AGG 0.472919 8.1:-25111398 MS.gene44440:CDS
GCCGATGAATGAAGATTTGA+AGG 0.493342 8.1:+25111472 None:intergenic
TGGGGTGTAGTGAATGGTAG+AGG 0.500971 8.1:+25111505 None:intergenic
ACCTTCAAATCTTCATTCAT+CGG 0.522192 8.1:-25111473 MS.gene44440:CDS
GGGGTAAGTGTGTGAATTCG+GGG 0.552268 8.1:+25111529 None:intergenic
GGTGTGATAGATGGATGAAA+AGG 0.565786 8.1:+25111551 None:intergenic
GCCTAGATTCTCTGCAACAG+TGG 0.569349 8.1:+25111397 None:intergenic
GTGTAGTGAATGGTAGAGGA+GGG 0.571659 8.1:+25111509 None:intergenic
GGTGTAGTGAATGGTAGAGG+AGG 0.617740 8.1:+25111508 None:intergenic
CCTAGATTCTCTGCAACAGT+GGG 0.622553 8.1:+25111398 None:intergenic
GGGTAAGTGTGTGAATTCGG+GGG 0.635427 8.1:+25111530 None:intergenic
TGTAGTGAATGGTAGAGGAG+GGG 0.653187 8.1:+25111510 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGAAGGTATTAGATTTT+GGG + chr8.1:25111429-25111448 None:intergenic 20.0%
!!! GATTTGAAGGTATTAGATTT+TGG + chr8.1:25111430-25111449 None:intergenic 25.0%
!!! TTTGAAGGTATTAGATTTTG+GGG + chr8.1:25111428-25111447 None:intergenic 25.0%
ACCTTCAAATCTTCATTCAT+CGG - chr8.1:25111439-25111458 MS.gene44440:CDS 30.0%
TGTCATGTTCAAAGATCTTT+GGG - chr8.1:25111570-25111589 MS.gene44440:CDS 30.0%
AGAAAGAAGTCGTTTGTTTC+GGG + chr8.1:25111467-25111486 None:intergenic 35.0%
CAGAAAGAAGTCGTTTGTTT+CGG + chr8.1:25111468-25111487 None:intergenic 35.0%
CTGTCATGTTCAAAGATCTT+TGG - chr8.1:25111569-25111588 MS.gene44440:CDS 35.0%
! TTAGTATTTTCACTGCTGCT+GGG - chr8.1:25111542-25111561 MS.gene44440:CDS 35.0%
GCCGATGAATGAAGATTTGA+AGG + chr8.1:25111443-25111462 None:intergenic 40.0%
GGTGTGATAGATGGATGAAA+AGG + chr8.1:25111364-25111383 None:intergenic 40.0%
! GTTAGTATTTTCACTGCTGC+TGG - chr8.1:25111541-25111560 MS.gene44440:CDS 40.0%
! TAGTATTTTCACTGCTGCTG+GGG - chr8.1:25111543-25111562 MS.gene44440:CDS 40.0%
!! GTTCAAAGATCTTTGGGATC+AGG - chr8.1:25111576-25111595 MS.gene44440:CDS 40.0%
!!! AGATTTTGGGGTGTAGTGAA+TGG + chr8.1:25111416-25111435 None:intergenic 40.0%
AGGGGTAAGTGTGTGAATTC+GGG + chr8.1:25111387-25111406 None:intergenic 45.0%
CCTAGATTCTCTGCAACAGT+GGG + chr8.1:25111517-25111536 None:intergenic 45.0%
GAGGGGTAAGTGTGTGAATT+CGG + chr8.1:25111388-25111407 None:intergenic 45.0%
GTGTAGTGAATGGTAGAGGA+GGG + chr8.1:25111406-25111425 None:intergenic 45.0%
TGTAGTGAATGGTAGAGGAG+GGG + chr8.1:25111405-25111424 None:intergenic 45.0%
CCCACTGTTGCAGAGAATCT+AGG - chr8.1:25111514-25111533 MS.gene44440:CDS 50.0%
GCCTAGATTCTCTGCAACAG+TGG + chr8.1:25111518-25111537 None:intergenic 50.0%
GGGGTAAGTGTGTGAATTCG+GGG + chr8.1:25111386-25111405 None:intergenic 50.0%
GGGTAAGTGTGTGAATTCGG+GGG + chr8.1:25111385-25111404 None:intergenic 50.0%
GGTGTAGTGAATGGTAGAGG+AGG + chr8.1:25111407-25111426 None:intergenic 50.0%
TGGGGTGTAGTGAATGGTAG+AGG + chr8.1:25111410-25111429 None:intergenic 50.0%
! GAATTCGGGGGTGTGATAGA+TGG + chr8.1:25111373-25111392 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25111331 25111603 25111331 ID=MS.gene44440
chr8.1 mRNA 25111331 25111603 25111331 ID=MS.gene44440.t1;Parent=MS.gene44440
chr8.1 exon 25111331 25111603 25111331 ID=MS.gene44440.t1.exon1;Parent=MS.gene44440.t1
chr8.1 CDS 25111331 25111603 25111331 ID=cds.MS.gene44440.t1;Parent=MS.gene44440.t1
Gene Sequence

>MS.gene44440

ATGGCAACAACTCTACAATCTCTTCGATTTCCTTTTCATCCATCTATCACACCCCCGAATTCACACACTTACCCCTCCTCTACCATTCACTACACCCCAAAATCTAATACCTTCAAATCTTCATTCATCGGCACCCGAAACAAACGACTTCTTTCTGCTAGAGCTTCGAGTTCACAGTATAGTCCCACTGTTGCAGAGAATCTAGGCGATGTTAGTATTTTCACTGCTGCTGGGGAATCTGTCATGTTCAAAGATCTTTGGGATCAGGAACAG

Protein sequence

>MS.gene44440.t1

MATTLQSLRFPFHPSITPPNSHTYPSSTIHYTPKSNTFKSSFIGTRNKRLLSARASSSQYSPTVAENLGDVSIFTAAGESVMFKDLWDQEQ