Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55699.t1 | XP_003629763.2 | 94.6 | 259 | 3 | 1 | 1 | 248 | 1 | 259 | 1.80E-127 | 465.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55699.t1 | Q96452 | 85.3 | 259 | 26 | 2 | 1 | 248 | 1 | 258 | 1.5e-114 | 413.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55699.t1 | G7LIR4 | 94.6 | 259 | 3 | 1 | 1 | 248 | 1 | 259 | 1.3e-127 | 465.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene55699 | 0.851408 | 9.05E-61 | -1.69E-46 |
| MS.gene04919 | MS.gene55699 | 0.824463 | 7.96E-54 | -1.69E-46 |
| MS.gene04920 | MS.gene55699 | 0.854146 | 1.50E-61 | -1.69E-46 |
| MS.gene049254 | MS.gene55699 | 0.820243 | 7.60E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene55699 | 0.871198 | 8.21E-67 | -1.69E-46 |
| MS.gene049368 | MS.gene55699 | 0.803775 | 2.97E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene55699 | -0.80792 | 3.99E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene55699 | 0.834891 | 2.31E-56 | -1.69E-46 |
| MS.gene049653 | MS.gene55699 | 0.816663 | 4.92E-52 | -1.69E-46 |
| MS.gene049680 | MS.gene55699 | 0.846294 | 2.38E-59 | -1.69E-46 |
| MS.gene049983 | MS.gene55699 | 0.858714 | 6.81E-63 | -1.69E-46 |
| MS.gene050384 | MS.gene55699 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene55699 | 0.828807 | 7.32E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene55699 | 0.817549 | 3.11E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene55699 | 0.808577 | 2.89E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene55699 | 0.815881 | 7.37E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene55699 | 0.805368 | 1.38E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene55699 | 0.83087 | 2.30E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene55699 | 0.804604 | 1.99E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene55699 | 0.848259 | 6.87E-60 | -1.69E-46 |
| MS.gene050955 | MS.gene55699 | 0.810826 | 9.50E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene55699 | 0.844143 | 9.07E-59 | -1.69E-46 |
| MS.gene051154 | MS.gene55699 | 0.823455 | 1.37E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene55699 | 0.810979 | 8.80E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene55699 | 0.838147 | 3.42E-57 | -1.69E-46 |
| MS.gene051363 | MS.gene55699 | 0.805056 | 1.60E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene55699 | 0.84226 | 2.88E-58 | -1.69E-46 |
| MS.gene051637 | MS.gene55699 | 0.804109 | 2.53E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene55699 | 0.838583 | 2.64E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene55699 | 0.806468 | 8.11E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene55699 | 0.838593 | 2.62E-57 | -1.69E-46 |
| MS.gene052072 | MS.gene55699 | 0.830088 | 3.58E-55 | -1.69E-46 |
| MS.gene052108 | MS.gene55699 | 0.831124 | 1.99E-55 | -1.69E-46 |
| MS.gene052500 | MS.gene55699 | 0.823092 | 1.67E-53 | -1.69E-46 |
| MS.gene052502 | MS.gene55699 | 0.811438 | 7.00E-51 | -1.69E-46 |
| MS.gene052518 | MS.gene55699 | 0.802896 | 4.51E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene55699 | 0.851847 | 6.80E-61 | -1.69E-46 |
| MS.gene052793 | MS.gene55699 | 0.80631 | 8.75E-50 | -1.69E-46 |
| MS.gene052826 | MS.gene55699 | 0.817012 | 4.11E-52 | -1.69E-46 |
| MS.gene052954 | MS.gene55699 | 0.85197 | 6.27E-61 | -1.69E-46 |
| MS.gene053006 | MS.gene55699 | 0.856205 | 3.76E-62 | -1.69E-46 |
| MS.gene053058 | MS.gene55699 | 0.825554 | 4.40E-54 | -1.69E-46 |
| MS.gene053228 | MS.gene55699 | 0.803351 | 3.63E-49 | -1.69E-46 |
| MS.gene053272 | MS.gene55699 | -0.819657 | 1.04E-52 | -1.69E-46 |
| MS.gene053575 | MS.gene55699 | 0.818206 | 2.21E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene55699 | 0.815323 | 9.80E-52 | -1.69E-46 |
| MS.gene053879 | MS.gene55699 | 0.853342 | 2.55E-61 | -1.69E-46 |
| MS.gene053931 | MS.gene55699 | 0.860636 | 1.80E-63 | -1.69E-46 |
| MS.gene053963 | MS.gene55699 | 0.839158 | 1.87E-57 | -1.69E-46 |
| MS.gene054004 | MS.gene55699 | 0.839967 | 1.15E-57 | -1.69E-46 |
| MS.gene054403 | MS.gene55699 | 0.808994 | 2.36E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene55699 | 0.823191 | 1.58E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene55699 | 0.82628 | 2.96E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene55699 | 0.831579 | 1.54E-55 | -1.69E-46 |
| MS.gene054893 | MS.gene55699 | 0.83927 | 1.75E-57 | -1.69E-46 |
| MS.gene054957 | MS.gene55699 | 0.8454 | 4.16E-59 | -1.69E-46 |
| MS.gene055150 | MS.gene55699 | 0.869826 | 2.31E-66 | -1.69E-46 |
| MS.gene05517 | MS.gene55699 | 0.815147 | 1.07E-51 | -1.69E-46 |
| MS.gene055222 | MS.gene55699 | 0.825421 | 4.73E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene55699 | 0.816997 | 4.14E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene55699 | 0.858406 | 8.42E-63 | -1.69E-46 |
| MS.gene056071 | MS.gene55699 | 0.836702 | 8.01E-57 | -1.69E-46 |
| MS.gene056363 | MS.gene55699 | 0.80427 | 2.34E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene55699 | 0.855118 | 7.82E-62 | -1.69E-46 |
| MS.gene056776 | MS.gene55699 | 0.809694 | 1.67E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene55699 | 0.842651 | 2.27E-58 | -1.69E-46 |
| MS.gene05689 | MS.gene55699 | 0.803953 | 2.73E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene55699 | 0.840195 | 1.01E-57 | -1.69E-46 |
| MS.gene057034 | MS.gene55699 | 0.820453 | 6.81E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene55699 | 0.846443 | 2.17E-59 | -1.69E-46 |
| MS.gene057155 | MS.gene55699 | 0.8083 | 3.31E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene55699 | 0.878894 | 1.94E-69 | -1.69E-46 |
| MS.gene057392 | MS.gene55699 | 0.859578 | 3.75E-63 | -1.69E-46 |
| MS.gene057622 | MS.gene55699 | 0.84811 | 7.55E-60 | -1.69E-46 |
| MS.gene057705 | MS.gene55699 | 0.803779 | 2.96E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene55699 | 0.834901 | 2.29E-56 | -1.69E-46 |
| MS.gene058008 | MS.gene55699 | 0.853837 | 1.83E-61 | -1.69E-46 |
| MS.gene058009 | MS.gene55699 | 0.844816 | 5.98E-59 | -1.69E-46 |
| MS.gene058095 | MS.gene55699 | 0.826321 | 2.89E-54 | -1.69E-46 |
| MS.gene058126 | MS.gene55699 | 0.807732 | 4.38E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene55699 | 0.82623 | 3.04E-54 | -1.69E-46 |
| MS.gene058292 | MS.gene55699 | 0.801987 | 6.95E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene55699 | 0.838123 | 3.47E-57 | -1.69E-46 |
| MS.gene058781 | MS.gene55699 | 0.87018 | 1.77E-66 | -1.69E-46 |
| MS.gene058783 | MS.gene55699 | 0.865083 | 7.64E-65 | -1.69E-46 |
| MS.gene05878 | MS.gene55699 | 0.839686 | 1.37E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene55699 | 0.806207 | 9.20E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene55699 | 0.876055 | 1.89E-68 | -1.69E-46 |
| MS.gene059436 | MS.gene55699 | 0.802992 | 4.31E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene55699 | 0.868823 | 4.90E-66 | -1.69E-46 |
| MS.gene05956 | MS.gene55699 | 0.811488 | 6.83E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene55699 | -0.873793 | 1.12E-67 | -1.69E-46 |
| MS.gene059901 | MS.gene55699 | -0.847779 | 9.32E-60 | -1.69E-46 |
| MS.gene059935 | MS.gene55699 | 0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene060244 | MS.gene55699 | 0.867366 | 1.44E-65 | -1.69E-46 |
| MS.gene060259 | MS.gene55699 | 0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene55699 | 0.815306 | 9.89E-52 | -1.69E-46 |
| MS.gene060522 | MS.gene55699 | 0.800006 | 1.76E-48 | -1.69E-46 |
| MS.gene060527 | MS.gene55699 | 0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene55699 | 0.81648 | 5.41E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene55699 | 0.835915 | 1.27E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene55699 | 0.895478 | 9.23E-76 | -1.69E-46 |
| MS.gene060937 | MS.gene55699 | 0.891845 | 2.74E-74 | -1.69E-46 |
| MS.gene061100 | MS.gene55699 | 0.810627 | 1.05E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene55699 | 0.813407 | 2.60E-51 | -1.69E-46 |
| MS.gene061243 | MS.gene55699 | 0.814058 | 1.87E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55699.t1 | MTR_8g086270 | 94.595 | 259 | 3 | 1 | 1 | 248 | 1 | 259 | 1.48e-179 | 493 |
| MS.gene55699.t1 | MTR_2g091225 | 80.078 | 256 | 40 | 1 | 1 | 245 | 1 | 256 | 2.33e-155 | 432 |
| MS.gene55699.t1 | MTR_2g015980 | 80.159 | 252 | 39 | 2 | 8 | 248 | 6 | 257 | 1.23e-147 | 412 |
| MS.gene55699.t1 | MTR_2g076960 | 77.165 | 254 | 46 | 2 | 2 | 244 | 3 | 255 | 2.39e-143 | 402 |
| MS.gene55699.t1 | MTR_2g091225 | 78.448 | 232 | 39 | 1 | 25 | 245 | 2 | 233 | 1.26e-137 | 386 |
| MS.gene55699.t1 | MTR_4g083060 | 76.305 | 249 | 48 | 1 | 1 | 238 | 1 | 249 | 1.17e-136 | 386 |
| MS.gene55699.t1 | MTR_5g044160 | 75.102 | 245 | 50 | 1 | 1 | 234 | 1 | 245 | 3.58e-134 | 379 |
| MS.gene55699.t1 | MTR_5g064580 | 69.136 | 243 | 62 | 2 | 7 | 236 | 8 | 250 | 3.30e-118 | 338 |
| MS.gene55699.t1 | MTR_3g099380 | 68.952 | 248 | 64 | 3 | 2 | 236 | 3 | 250 | 2.98e-117 | 336 |
| MS.gene55699.t1 | MTR_3g100620 | 62.241 | 241 | 77 | 2 | 7 | 233 | 11 | 251 | 5.32e-105 | 304 |
| MS.gene55699.t1 | MTR_3g099380 | 64.677 | 201 | 58 | 2 | 2 | 189 | 3 | 203 | 4.89e-87 | 257 |
| MS.gene55699.t1 | MTR_0137s0020 | 50.207 | 241 | 103 | 4 | 7 | 233 | 11 | 248 | 2.15e-69 | 214 |
| MS.gene55699.t1 | MTR_3g014060 | 48.988 | 247 | 109 | 4 | 1 | 233 | 5 | 248 | 2.36e-68 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55699.t1 | AT2G42590 | 75.096 | 261 | 52 | 2 | 1 | 248 | 1 | 261 | 2.03e-143 | 402 |
| MS.gene55699.t1 | AT2G42590 | 74.809 | 262 | 52 | 3 | 1 | 248 | 1 | 262 | 1.32e-141 | 397 |
| MS.gene55699.t1 | AT2G42590 | 74.809 | 262 | 52 | 3 | 1 | 248 | 1 | 262 | 1.36e-141 | 398 |
| MS.gene55699.t1 | AT1G26480 | 74.590 | 244 | 51 | 1 | 2 | 234 | 5 | 248 | 6.28e-135 | 381 |
| MS.gene55699.t1 | AT1G22300 | 74.793 | 242 | 50 | 1 | 6 | 236 | 4 | 245 | 2.51e-127 | 361 |
| MS.gene55699.t1 | AT1G22300 | 74.793 | 242 | 50 | 1 | 6 | 236 | 4 | 245 | 2.62e-127 | 361 |
| MS.gene55699.t1 | AT1G34760 | 71.369 | 241 | 58 | 1 | 6 | 235 | 4 | 244 | 2.25e-125 | 356 |
| MS.gene55699.t1 | AT1G34760 | 71.369 | 241 | 58 | 1 | 6 | 235 | 4 | 244 | 2.26e-125 | 356 |
| MS.gene55699.t1 | AT1G22300 | 75.105 | 237 | 48 | 1 | 6 | 231 | 4 | 240 | 6.93e-125 | 355 |
| MS.gene55699.t1 | AT1G35160 | 68.145 | 248 | 66 | 2 | 2 | 236 | 6 | 253 | 2.00e-118 | 339 |
| MS.gene55699.t1 | AT1G78300 | 69.136 | 243 | 62 | 3 | 7 | 236 | 5 | 247 | 5.70e-118 | 337 |
| MS.gene55699.t1 | AT5G38480 | 71.250 | 240 | 56 | 3 | 7 | 233 | 4 | 243 | 4.52e-117 | 335 |
| MS.gene55699.t1 | AT5G38480 | 71.250 | 240 | 56 | 3 | 7 | 233 | 4 | 243 | 4.52e-117 | 335 |
| MS.gene55699.t1 | AT1G35160 | 69.874 | 239 | 59 | 3 | 2 | 227 | 6 | 244 | 1.08e-116 | 335 |
| MS.gene55699.t1 | AT5G38480 | 68.775 | 253 | 65 | 4 | 7 | 246 | 4 | 255 | 1.30e-116 | 334 |
| MS.gene55699.t1 | AT4G09000 | 67.769 | 242 | 65 | 3 | 2 | 230 | 5 | 246 | 2.72e-116 | 333 |
| MS.gene55699.t1 | AT3G02520 | 69.835 | 242 | 58 | 4 | 7 | 233 | 5 | 246 | 7.23e-115 | 330 |
| MS.gene55699.t1 | AT3G02520 | 69.835 | 242 | 58 | 4 | 7 | 233 | 5 | 246 | 7.23e-115 | 330 |
| MS.gene55699.t1 | AT4G09000 | 68.201 | 239 | 63 | 3 | 2 | 227 | 5 | 243 | 3.19e-114 | 330 |
| MS.gene55699.t1 | AT5G65430 | 65.992 | 247 | 70 | 2 | 1 | 233 | 1 | 247 | 3.55e-114 | 327 |
| MS.gene55699.t1 | AT5G10450 | 65.182 | 247 | 72 | 2 | 1 | 233 | 1 | 247 | 2.02e-113 | 325 |
| MS.gene55699.t1 | AT5G65430 | 65.984 | 244 | 69 | 2 | 1 | 230 | 1 | 244 | 9.85e-113 | 324 |
| MS.gene55699.t1 | AT5G10450 | 65.164 | 244 | 71 | 2 | 1 | 230 | 1 | 244 | 3.87e-112 | 322 |
| MS.gene55699.t1 | AT5G65430 | 66.390 | 241 | 67 | 2 | 1 | 227 | 1 | 241 | 1.04e-111 | 322 |
| MS.gene55699.t1 | AT5G10450 | 65.560 | 241 | 69 | 2 | 1 | 227 | 1 | 241 | 2.40e-111 | 321 |
| MS.gene55699.t1 | AT5G10450 | 65.560 | 241 | 69 | 2 | 1 | 227 | 1 | 241 | 7.31e-111 | 320 |
| MS.gene55699.t1 | AT5G16050 | 63.855 | 249 | 77 | 2 | 1 | 236 | 1 | 249 | 1.87e-107 | 311 |
| MS.gene55699.t1 | AT5G16050 | 63.855 | 249 | 77 | 2 | 1 | 236 | 1 | 249 | 1.87e-107 | 311 |
| MS.gene55699.t1 | AT1G78220 | 51.293 | 232 | 101 | 1 | 6 | 225 | 4 | 235 | 6.62e-80 | 240 |
| MS.gene55699.t1 | AT1G22290 | 49.758 | 207 | 83 | 5 | 6 | 203 | 4 | 198 | 9.98e-56 | 178 |
| MS.gene55699.t1 | AT1G22290 | 49.510 | 204 | 82 | 5 | 6 | 200 | 4 | 195 | 1.92e-55 | 176 |
| MS.gene55699.t1 | AT2G10450 | 61.702 | 47 | 17 | 1 | 193 | 238 | 16 | 62 | 3.56e-11 | 58.2 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57369 | gene | 12297 | 21758 | 12297 | ID=MS.gene55699 |
| 57369 | mRNA | 12297 | 21758 | 12297 | ID=MS.gene55699.t1;Parent=MS.gene55699 |
| 57369 | exon | 12297 | 12369 | 12297 | ID=MS.gene55699.t1.exon1;Parent=MS.gene55699.t1 |
| 57369 | CDS | 12297 | 12369 | 12297 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| 57369 | exon | 19020 | 19298 | 19020 | ID=MS.gene55699.t1.exon2;Parent=MS.gene55699.t1 |
| 57369 | CDS | 19020 | 19298 | 19020 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| 57369 | exon | 19332 | 19414 | 19332 | ID=MS.gene55699.t1.exon3;Parent=MS.gene55699.t1 |
| 57369 | CDS | 19332 | 19414 | 19332 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| 57369 | exon | 20463 | 20572 | 20463 | ID=MS.gene55699.t1.exon4;Parent=MS.gene55699.t1 |
| 57369 | CDS | 20463 | 20572 | 20463 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| 57369 | exon | 20656 | 20804 | 20656 | ID=MS.gene55699.t1.exon5;Parent=MS.gene55699.t1 |
| 57369 | CDS | 20656 | 20804 | 20656 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| 57369 | exon | 21706 | 21758 | 21706 | ID=MS.gene55699.t1.exon6;Parent=MS.gene55699.t1 |
| 57369 | CDS | 21706 | 21758 | 21706 | ID=cds.MS.gene55699.t1;Parent=MS.gene55699.t1 |
| Gene Sequence |
| Protein sequence |
