Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067697.t1 | XP_024636075.1 | 84.8 | 877 | 123 | 5 | 1 | 872 | 1 | 872 | 0.00E+00 | 1192.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067697.t1 | Q93ZG9 | 57.1 | 119 | 51 | 0 | 753 | 871 | 358 | 476 | 6.2e-35 | 151.0 |
| MS.gene067697.t1 | Q93ZG9 | 45.7 | 94 | 50 | 1 | 1 | 93 | 1 | 94 | 1.3e-19 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067697.t1 | G7JN90 | 84.0 | 876 | 130 | 5 | 1 | 872 | 1 | 870 | 0.0e+00 | 1172.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049129 | MS.gene067697 | -0.813833 | 2.10E-51 | -1.69E-46 |
| MS.gene049142 | MS.gene067697 | 0.82574 | 3.98E-54 | -1.69E-46 |
| MS.gene04918 | MS.gene067697 | 0.852067 | 5.89E-61 | -1.69E-46 |
| MS.gene04920 | MS.gene067697 | 0.830387 | 3.02E-55 | -1.69E-46 |
| MS.gene049254 | MS.gene067697 | 0.809614 | 1.73E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene067697 | 0.801593 | 8.37E-49 | -1.69E-46 |
| MS.gene049343 | MS.gene067697 | 0.802076 | 6.66E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene067697 | 0.81635 | 5.79E-52 | -1.69E-46 |
| MS.gene049368 | MS.gene067697 | 0.813569 | 2.40E-51 | -1.69E-46 |
| MS.gene049411 | MS.gene067697 | 0.807679 | 4.49E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene067697 | 0.834702 | 2.57E-56 | -1.69E-46 |
| MS.gene049824 | MS.gene067697 | 0.816138 | 6.45E-52 | -1.69E-46 |
| MS.gene049825 | MS.gene067697 | 0.837664 | 4.55E-57 | -1.69E-46 |
| MS.gene049873 | MS.gene067697 | 0.828843 | 7.18E-55 | -1.69E-46 |
| MS.gene049983 | MS.gene067697 | 0.807927 | 3.98E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene067697 | 0.817804 | 2.73E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene067697 | 0.852665 | 3.98E-61 | -1.69E-46 |
| MS.gene051234 | MS.gene067697 | 0.845101 | 5.01E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene067697 | 0.824717 | 6.94E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene067697 | 0.803966 | 2.71E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene067697 | 0.831049 | 2.08E-55 | -1.69E-46 |
| MS.gene051363 | MS.gene067697 | 0.846026 | 2.81E-59 | -1.69E-46 |
| MS.gene051365 | MS.gene067697 | 0.830707 | 2.52E-55 | -1.69E-46 |
| MS.gene051597 | MS.gene067697 | 0.81241 | 4.30E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene067697 | 0.826309 | 2.91E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene067697 | 0.858368 | 8.65E-63 | -1.69E-46 |
| MS.gene051975 | MS.gene067697 | 0.832267 | 1.04E-55 | -1.69E-46 |
| MS.gene052072 | MS.gene067697 | 0.824111 | 9.63E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene067697 | 0.800776 | 1.23E-48 | -1.69E-46 |
| MS.gene052518 | MS.gene067697 | 0.850446 | 1.69E-60 | -1.69E-46 |
| MS.gene052519 | MS.gene067697 | 0.838121 | 3.47E-57 | -1.69E-46 |
| MS.gene052582 | MS.gene067697 | 0.806597 | 7.61E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene067697 | 0.824158 | 9.39E-54 | -1.69E-46 |
| MS.gene052717 | MS.gene067697 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene067697 | 0.80124 | 9.88E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene067697 | 0.836783 | 7.64E-57 | -1.69E-46 |
| MS.gene053006 | MS.gene067697 | 0.800482 | 1.41E-48 | -1.69E-46 |
| MS.gene053008 | MS.gene067697 | 0.822214 | 2.67E-53 | -1.69E-46 |
| MS.gene053058 | MS.gene067697 | 0.817403 | 3.36E-52 | -1.69E-46 |
| MS.gene053080 | MS.gene067697 | 0.809048 | 2.29E-50 | -1.69E-46 |
| MS.gene053122 | MS.gene067697 | 0.864302 | 1.34E-64 | -1.69E-46 |
| MS.gene053226 | MS.gene067697 | 0.8689 | 4.63E-66 | -1.69E-46 |
| MS.gene053272 | MS.gene067697 | -0.810718 | 1.00E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene067697 | 0.822102 | 2.83E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene067697 | 0.815979 | 7.00E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene067697 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene054142 | MS.gene067697 | 0.821922 | 3.12E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene067697 | 0.800744 | 1.25E-48 | -1.69E-46 |
| MS.gene05458 | MS.gene067697 | 0.82877 | 7.47E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene067697 | 0.841243 | 5.35E-58 | -1.69E-46 |
| MS.gene054657 | MS.gene067697 | 0.803699 | 3.08E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene067697 | 0.823077 | 1.68E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene067697 | 0.830979 | 2.16E-55 | -1.69E-46 |
| MS.gene055555 | MS.gene067697 | 0.862079 | 6.53E-64 | -1.69E-46 |
| MS.gene055580 | MS.gene067697 | -0.830936 | 2.22E-55 | -1.69E-46 |
| MS.gene055694 | MS.gene067697 | 0.800022 | 1.75E-48 | -1.69E-46 |
| MS.gene055808 | MS.gene067697 | 0.808427 | 3.11E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene067697 | 0.818731 | 1.68E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene067697 | -0.832639 | 8.43E-56 | -1.69E-46 |
| MS.gene056091 | MS.gene067697 | 0.801842 | 7.44E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene067697 | 0.822729 | 2.03E-53 | -1.69E-46 |
| MS.gene056403 | MS.gene067697 | 0.839601 | 1.44E-57 | -1.69E-46 |
| MS.gene056595 | MS.gene067697 | 0.810002 | 1.43E-50 | -1.69E-46 |
| MS.gene056605 | MS.gene067697 | 0.831372 | 1.73E-55 | -1.69E-46 |
| MS.gene056786 | MS.gene067697 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene067697 | 0.801608 | 8.31E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene067697 | 0.854548 | 1.14E-61 | -1.69E-46 |
| MS.gene056986 | MS.gene067697 | 0.828871 | 7.07E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene067697 | 0.860189 | 2.46E-63 | -1.69E-46 |
| MS.gene057208 | MS.gene067697 | 0.822312 | 2.53E-53 | -1.69E-46 |
| MS.gene057705 | MS.gene067697 | 0.837904 | 3.95E-57 | -1.69E-46 |
| MS.gene05781 | MS.gene067697 | 0.83912 | 1.92E-57 | -1.69E-46 |
| MS.gene058008 | MS.gene067697 | 0.814873 | 1.23E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene067697 | 0.819773 | 9.74E-53 | -1.69E-46 |
| MS.gene058088 | MS.gene067697 | 0.812635 | 3.84E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene067697 | 0.800269 | 1.56E-48 | -1.69E-46 |
| MS.gene058625 | MS.gene067697 | 0.814293 | 1.66E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene067697 | 0.841717 | 4.01E-58 | -1.69E-46 |
| MS.gene058783 | MS.gene067697 | 0.865259 | 6.72E-65 | -1.69E-46 |
| MS.gene059146 | MS.gene067697 | 0.877711 | 5.05E-69 | -1.69E-46 |
| MS.gene05950 | MS.gene067697 | 0.811298 | 7.51E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene067697 | 0.823909 | 1.07E-53 | -1.69E-46 |
| MS.gene059625 | MS.gene067697 | 0.802438 | 5.61E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene067697 | 0.839171 | 1.86E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene067697 | -0.846916 | 1.61E-59 | -1.69E-46 |
| MS.gene059901 | MS.gene067697 | -0.872285 | 3.58E-67 | -1.69E-46 |
| MS.gene059935 | MS.gene067697 | 0.880927 | 3.67E-70 | -1.69E-46 |
| MS.gene06011 | MS.gene067697 | 0.844585 | 6.90E-59 | -1.69E-46 |
| MS.gene060365 | MS.gene067697 | 0.833104 | 6.46E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene067697 | 0.858074 | 1.06E-62 | -1.69E-46 |
| MS.gene060512 | MS.gene067697 | 0.802331 | 5.90E-49 | -1.69E-46 |
| MS.gene060586 | MS.gene067697 | 0.818157 | 2.27E-52 | -1.69E-46 |
| MS.gene060763 | MS.gene067697 | 0.889848 | 1.68E-73 | -1.69E-46 |
| MS.gene060830 | MS.gene067697 | 0.851095 | 1.11E-60 | -1.69E-46 |
| MS.gene060935 | MS.gene067697 | 0.861722 | 8.40E-64 | -1.69E-46 |
| MS.gene060937 | MS.gene067697 | 0.852738 | 3.79E-61 | -1.69E-46 |
| MS.gene06094 | MS.gene067697 | 0.804991 | 1.66E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene067697 | 0.840043 | 1.10E-57 | -1.69E-46 |
| MS.gene061160 | MS.gene067697 | 0.804162 | 2.47E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene067697 | 0.818672 | 1.73E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067697.t1 | MTR_4g032680 | 92.930 | 877 | 50 | 6 | 1 | 872 | 1 | 870 | 0.0 | 1482 |
| MS.gene067697.t1 | MTR_4g032680 | 92.711 | 878 | 50 | 8 | 1 | 872 | 1 | 870 | 0.0 | 1471 |
| MS.gene067697.t1 | MTR_4g032680 | 88.525 | 549 | 53 | 4 | 325 | 872 | 222 | 761 | 0.0 | 867 |
| MS.gene067697.t1 | MTR_4g032680 | 89.220 | 436 | 37 | 4 | 1 | 433 | 1 | 429 | 0.0 | 692 |
| MS.gene067697.t1 | MTR_4g032680 | 91.629 | 442 | 35 | 2 | 432 | 872 | 221 | 661 | 0.0 | 747 |
| MS.gene067697.t1 | MTR_4g032680 | 86.322 | 329 | 29 | 4 | 1 | 319 | 1 | 323 | 0.0 | 538 |
| MS.gene067697.t1 | MTR_5g008680 | 58.824 | 119 | 48 | 1 | 753 | 871 | 382 | 499 | 1.91e-39 | 154 |
| MS.gene067697.t1 | MTR_5g008680 | 37.778 | 225 | 122 | 7 | 1 | 213 | 1 | 219 | 6.62e-33 | 134 |
| MS.gene067697.t1 | MTR_8g012380 | 41.573 | 89 | 52 | 0 | 784 | 872 | 50 | 138 | 3.01e-14 | 71.2 |
| MS.gene067697.t1 | MTR_1g061850 | 35.484 | 124 | 66 | 5 | 757 | 871 | 125 | 243 | 4.77e-14 | 73.6 |
| MS.gene067697.t1 | MTR_8g038785 | 41.176 | 85 | 48 | 1 | 787 | 869 | 191 | 275 | 1.37e-13 | 75.1 |
| MS.gene067697.t1 | MTR_7g053470 | 36.047 | 86 | 55 | 0 | 784 | 869 | 52 | 137 | 2.36e-12 | 70.9 |
| MS.gene067697.t1 | MTR_8g080980 | 37.209 | 86 | 54 | 0 | 784 | 869 | 56 | 141 | 1.07e-11 | 68.9 |
| MS.gene067697.t1 | MTR_8g012340 | 36.782 | 87 | 55 | 0 | 783 | 869 | 57 | 143 | 1.87e-11 | 68.2 |
| MS.gene067697.t1 | MTR_4g094785 | 38.583 | 127 | 61 | 6 | 756 | 869 | 115 | 237 | 2.90e-11 | 65.1 |
| MS.gene067697.t1 | MTR_5g080300 | 35.714 | 84 | 53 | 1 | 787 | 869 | 35 | 118 | 5.68e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067697.t1 | AT3G12340 | 51.562 | 192 | 82 | 5 | 1 | 183 | 1 | 190 | 7.55e-44 | 167 |
| MS.gene067697.t1 | AT3G12340 | 31.467 | 375 | 199 | 10 | 510 | 872 | 171 | 499 | 1.65e-30 | 127 |
| MS.gene067697.t1 | AT4G25340 | 56.667 | 120 | 52 | 0 | 753 | 872 | 358 | 477 | 5.64e-41 | 157 |
| MS.gene067697.t1 | AT4G25340 | 33.036 | 224 | 132 | 6 | 1 | 211 | 1 | 219 | 8.61e-25 | 109 |
| MS.gene067697.t1 | AT3G12340 | 31.467 | 375 | 199 | 10 | 510 | 872 | 50 | 378 | 3.14e-31 | 127 |
| MS.gene067697.t1 | AT3G12340 | 31.467 | 375 | 199 | 10 | 510 | 872 | 50 | 378 | 3.14e-31 | 127 |
| MS.gene067697.t1 | AT5G05420 | 50.000 | 108 | 53 | 1 | 764 | 871 | 36 | 142 | 8.90e-30 | 115 |
| MS.gene067697.t1 | AT4G25340 | 33.036 | 224 | 132 | 6 | 1 | 211 | 1 | 219 | 4.15e-25 | 110 |
| MS.gene067697.t1 | AT4G25340 | 57.895 | 76 | 32 | 0 | 753 | 828 | 358 | 433 | 1.54e-22 | 102 |
| MS.gene067697.t1 | AT5G48580 | 41.573 | 89 | 52 | 0 | 784 | 872 | 52 | 140 | 9.83e-15 | 73.2 |
| MS.gene067697.t1 | AT3G10060 | 35.484 | 124 | 66 | 5 | 757 | 871 | 98 | 216 | 2.81e-14 | 73.6 |
| MS.gene067697.t1 | AT3G25220 | 42.697 | 89 | 51 | 0 | 784 | 872 | 52 | 140 | 3.34e-14 | 71.2 |
| MS.gene067697.t1 | AT5G48570 | 38.372 | 86 | 53 | 0 | 784 | 869 | 65 | 150 | 3.80e-13 | 73.6 |
| MS.gene067697.t1 | AT3G25230 | 37.931 | 87 | 54 | 0 | 784 | 870 | 57 | 143 | 4.55e-12 | 70.1 |
| MS.gene067697.t1 | AT3G25230 | 37.931 | 87 | 54 | 0 | 784 | 870 | 57 | 143 | 6.03e-12 | 69.7 |
Find 174 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTCCTTGCAATTGAATT+TGG | 0.202666 | 4.1:-68351080 | MS.gene067697:CDS |
| CCATTTCTGTTGTCACCTTC+AGG | 0.226295 | 4.1:+68346195 | None:intergenic |
| TCATAAACCAACCATGAATT+TGG | 0.244200 | 4.1:+68343008 | None:intergenic |
| GTTATTGAGGGTTGGGATAT+TGG | 0.252092 | 4.1:-68343326 | MS.gene067697:CDS |
| CTTCCACTGTTCCGGATGTT+GGG | 0.283057 | 4.1:-68348617 | MS.gene067697:CDS |
| CTTCCACTGTTCCGGATGTT+GGG | 0.283057 | 4.1:-68349486 | MS.gene067697:CDS |
| CTTGAGACAGGGAAAGAAAA+TGG | 0.283603 | 4.1:-68343678 | MS.gene067697:CDS |
| GGCAGTTCCTTCCACTGTTC+CGG | 0.304338 | 4.1:-68348625 | MS.gene067697:CDS |
| GACAGTTCCTTCCACTGTTC+CGG | 0.305801 | 4.1:-68349494 | MS.gene067697:CDS |
| AATGTTATTCTTTCCGTCCT+TGG | 0.324140 | 4.1:-68351040 | MS.gene067697:CDS |
| ATATTTGAGCTTGCCTTCAT+TGG | 0.326158 | 4.1:+68350109 | None:intergenic |
| GGTCGATCCTTGCACTGTTC+CGG | 0.327548 | 4.1:-68349081 | MS.gene067697:CDS |
| TTTCCTGATCAAAGTGATAC+TGG | 0.328927 | 4.1:+68349365 | None:intergenic |
| AGTAATCAGGACACAGTTAA+TGG | 0.330450 | 4.1:-68346920 | MS.gene067697:CDS |
| AATTGCTACAGTAGGGAAAA+AGG | 0.333662 | 4.1:-68343652 | MS.gene067697:intron |
| TTTCTTAACTTTCTTATCTT+CGG | 0.342081 | 4.1:+68343863 | None:intergenic |
| AGCTTGCCTTCATTGGAAAT+AGG | 0.344631 | 4.1:+68350116 | None:intergenic |
| GAACACAGACGAGGATCTTC+TGG | 0.344791 | 4.1:-68348484 | MS.gene067697:CDS |
| TAGTGCTACTGAAACAAAAT+TGG | 0.347050 | 4.1:-68349278 | MS.gene067697:CDS |
| AGATTTCTCTGTTTCTGTAT+TGG | 0.347847 | 4.1:+68350214 | None:intergenic |
| CCTTCCACTGTTCCGGATGT+TGG | 0.349134 | 4.1:-68348618 | MS.gene067697:CDS |
| CCTTCCACTGTTCCGGATGT+TGG | 0.349134 | 4.1:-68349487 | MS.gene067697:CDS |
| TTTGAATTTCAGGCCACTTT+GGG | 0.356978 | 4.1:-68351193 | MS.gene067697:intron |
| CATTTGACAATGTTCTAACT+TGG | 0.359569 | 4.1:+68343718 | None:intergenic |
| ATGAACACTCACTTATCATT+AGG | 0.360171 | 4.1:+68348435 | None:intergenic |
| AGGAGAAGTTATTGAGGGTT+GGG | 0.373313 | 4.1:-68343333 | MS.gene067697:CDS |
| TAAATCCTTTGTAAGGGTCT+TGG | 0.380961 | 4.1:-68349807 | MS.gene067697:CDS |
| CTTTGAATTTCAGGCCACTT+TGG | 0.383074 | 4.1:-68351194 | MS.gene067697:intron |
| AGAAGAATAAATCCTTTGTA+AGG | 0.383149 | 4.1:-68349814 | MS.gene067697:CDS |
| GAAGAAGAAAAGTAAAAGTC+AGG | 0.385488 | 4.1:-68343838 | MS.gene067697:CDS |
| CTTTGTAAGGGTCTTGGATC+AGG | 0.392305 | 4.1:-68349801 | MS.gene067697:CDS |
| TAAAGAGAGCACAGATAAAC+AGG | 0.395132 | 4.1:+68351115 | None:intergenic |
| TAAATCACATACGTCATTGA+TGG | 0.404187 | 4.1:+68343158 | None:intergenic |
| AAAACATACCACCAAATTCA+TGG | 0.412855 | 4.1:-68343019 | MS.gene067697:CDS |
| TGAAATGTGGAGACGACCTT+TGG | 0.413799 | 4.1:+68352289 | None:intergenic |
| TTCTTTCCGTCCTTGGCCCT+CGG | 0.414122 | 4.1:-68351033 | MS.gene067697:CDS |
| GGACTCTGCTGTCTCTTTGT+CGG | 0.416295 | 4.1:+68346146 | None:intergenic |
| CCTTGCACTGTTCCGGATGT+TGG | 0.419726 | 4.1:-68349074 | MS.gene067697:CDS |
| TTTCCTGATCCAAGAGATCC+TGG | 0.427168 | 4.1:+68348931 | None:intergenic |
| GTTATTCAAGAGCTTGAGAC+AGG | 0.428795 | 4.1:-68343690 | MS.gene067697:CDS |
| TATCTGTGCTCTCTTTATCC+AGG | 0.431922 | 4.1:-68351109 | MS.gene067697:CDS |
| TTCTTGCAAAGAGAAGAAAT+TGG | 0.432751 | 4.1:+68349833 | None:intergenic |
| AAGGAGAAGTTATTGAGGGT+TGG | 0.439237 | 4.1:-68343334 | MS.gene067697:CDS |
| TGTGTTCATTTCCCTGAGGC+AGG | 0.441265 | 4.1:+68346942 | None:intergenic |
| CAATGTTGTTAACCTGCCTC+AGG | 0.441931 | 4.1:-68346954 | MS.gene067697:CDS |
| TATCTTGCCCGTGGCCGCTA+TGG | 0.443510 | 4.1:-68350989 | MS.gene067697:CDS |
| CTGGCAAGTTGAAGGAAAAC+GGG | 0.443905 | 4.1:-68343496 | MS.gene067697:CDS |
| TGGCCGCTATGGCAATACTA+TGG | 0.452319 | 4.1:-68350978 | MS.gene067697:CDS |
| GGGAAATGAACACAGTAATC+AGG | 0.452685 | 4.1:-68346933 | MS.gene067697:CDS |
| GGTTGGGATATTGGTCTTGA+AGG | 0.456064 | 4.1:-68343317 | MS.gene067697:intron |
| ACTGGCAAGTTGAAGGAAAA+CGG | 0.458754 | 4.1:-68343497 | MS.gene067697:CDS |
| ATCGCCATTACTAGAACTCT+TGG | 0.460617 | 4.1:+68349004 | None:intergenic |
| GAACACGGCCCAGGATCTCT+TGG | 0.460735 | 4.1:-68348940 | MS.gene067697:CDS |
| GGATCTCTTGGATCAGGAAA+TGG | 0.461360 | 4.1:-68348928 | MS.gene067697:CDS |
| TCTTCGGATTGATTTCCATC+AGG | 0.465182 | 4.1:+68343879 | None:intergenic |
| TCACTGAGAGTCAGATTCAT+AGG | 0.468116 | 4.1:-68349673 | MS.gene067697:intron |
| CAGTGTGCATGTACAATCTC+AGG | 0.468383 | 4.1:-68349879 | MS.gene067697:CDS |
| TTGCGCAGCATTATCTAGTT+CGG | 0.468959 | 4.1:+68348977 | None:intergenic |
| CCTGAAGGTGACAACAGAAA+TGG | 0.473113 | 4.1:-68346195 | MS.gene067697:CDS |
| CAAGTTAGAACATTGTCAAA+TGG | 0.473879 | 4.1:-68343717 | MS.gene067697:CDS |
| AAACTCTGACGTGCCTGTAT+CGG | 0.474576 | 4.1:-68343802 | MS.gene067697:CDS |
| AAGGGTCTTGGATCAGGAAA+TGG | 0.474786 | 4.1:-68349795 | MS.gene067697:CDS |
| GTAATCAGGACACAGTTAAT+GGG | 0.477374 | 4.1:-68346919 | MS.gene067697:intron |
| GATGATGATAGCGGTCTTGA+AGG | 0.478274 | 4.1:-68349920 | MS.gene067697:CDS |
| GACAAAGAAGCAATTGATGC+TGG | 0.478437 | 4.1:-68349761 | MS.gene067697:CDS |
| TTTCAGTAGCACTATTCTCA+TGG | 0.478874 | 4.1:+68349287 | None:intergenic |
| TGCAAGGACTCAGTTTGTCC+TGG | 0.479083 | 4.1:+68351091 | None:intergenic |
| ATGTTCATTACTAGAGCTCT+TGG | 0.479995 | 4.1:+68348548 | None:intergenic |
| TTTCAATAATACTGTTCTCA+TGG | 0.480905 | 4.1:+68349720 | None:intergenic |
| GGCCCAGGATCTCTTGGATC+AGG | 0.487421 | 4.1:-68348934 | MS.gene067697:CDS |
| ACTTATCATTAGGTTGCTGC+TGG | 0.489995 | 4.1:+68348445 | None:intergenic |
| GTACTTTGCTCAACCGATAC+AGG | 0.491823 | 4.1:+68343789 | None:intergenic |
| CAGTGGAAGGAACTGCCAAC+TGG | 0.491931 | 4.1:+68348631 | None:intergenic |
| GACAATGTTCTAACTTGGGA+AGG | 0.493720 | 4.1:+68343723 | None:intergenic |
| GTTGGTTTATGACTTTGAAC+TGG | 0.494478 | 4.1:-68342997 | MS.gene067697:CDS |
| CAATTACACTGGCAAGTTGA+AGG | 0.496463 | 4.1:-68343504 | MS.gene067697:CDS |
| AGCTGAACCTTCTGATCACT+TGG | 0.500805 | 4.1:-68349102 | MS.gene067697:CDS |
| AATGGGAAAATTGCTACAGT+AGG | 0.508437 | 4.1:-68343660 | MS.gene067697:CDS |
| TTCACAGGTTGGTCAAGGAC+AGG | 0.509597 | 4.1:-68347050 | MS.gene067697:CDS |
| CATACCACCAAATTCATGGT+TGG | 0.511268 | 4.1:-68343015 | MS.gene067697:CDS |
| TAAAATACTACCTTCTTCAC+AGG | 0.511997 | 4.1:-68347065 | MS.gene067697:CDS |
| TTCCACTGTTCCGGATGTTG+GGG | 0.516382 | 4.1:-68348616 | MS.gene067697:CDS |
| TTCCACTGTTCCGGATGTTG+GGG | 0.516382 | 4.1:-68349485 | MS.gene067697:CDS |
| AGGTAAAGGAGAAGTTATTG+AGG | 0.516723 | 4.1:-68343339 | MS.gene067697:CDS |
| GAATCATAAGTGTGAGTGAA+AGG | 0.517316 | 4.1:+68352314 | None:intergenic |
| GGTAAAGGAGAAGTTATTGA+GGG | 0.518782 | 4.1:-68343338 | MS.gene067697:CDS |
| TTTCAGGCCACTTTGGGACA+TGG | 0.520724 | 4.1:-68351187 | MS.gene067697:intron |
| GCTCCTTTCAAATTTCGCCT+TGG | 0.521588 | 4.1:-68343446 | MS.gene067697:intron |
| GCAATTGAATTTGGAGCTTG+AGG | 0.523659 | 4.1:-68351071 | MS.gene067697:CDS |
| TTGAGACAGGGAAAGAAAAT+GGG | 0.525124 | 4.1:-68343677 | MS.gene067697:CDS |
| TAGTATTGCCATAGCGGCCA+CGG | 0.525730 | 4.1:+68350981 | None:intergenic |
| TGATTCTCAGGCATGCGAGT+TGG | 0.529832 | 4.1:-68343206 | MS.gene067697:intron |
| CCTTGACCAACCTGTGAAGA+AGG | 0.529915 | 4.1:+68347055 | None:intergenic |
| ACTGAGATGATGGAAGAGGA+TGG | 0.530349 | 4.1:-68343768 | MS.gene067697:CDS |
| AATTTGGAGCTTGAGGAAGA+TGG | 0.534366 | 4.1:-68351064 | MS.gene067697:CDS |
| TGAGCAAAGTACTGAGATGA+TGG | 0.540063 | 4.1:-68343778 | MS.gene067697:CDS |
| CACACTTATGATTCTTCCAA+AGG | 0.540990 | 4.1:-68352305 | MS.gene067697:CDS |
| ATTTGACAATGTTCTAACTT+GGG | 0.541426 | 4.1:+68343719 | None:intergenic |
| GTCCCAGTATCACTTTGATC+AGG | 0.542436 | 4.1:-68349368 | MS.gene067697:CDS |
| GAAGTACCACTTGGTAGAGT+CGG | 0.543598 | 4.1:-68349942 | MS.gene067697:CDS |
| CGCAGCATTATCTAGTTCGG+TGG | 0.543795 | 4.1:+68348980 | None:intergenic |
| TCGCAGATATTCAAACTCAT+AGG | 0.545219 | 4.1:-68348806 | MS.gene067697:intron |
| AGCTGAACCTTCAGACCAGT+TGG | 0.547478 | 4.1:-68348646 | MS.gene067697:CDS |
| GACAGAGAAACAGTTGATGC+TGG | 0.549982 | 4.1:-68349328 | MS.gene067697:CDS |
| GCATCTCCTATTTCCAATGA+AGG | 0.550943 | 4.1:-68350122 | MS.gene067697:intron |
| ACAATGGAAAGGGAATCATA+TGG | 0.551522 | 4.1:-68350248 | MS.gene067697:intron |
| TGAGAATCTGTCTCAGAGGA+AGG | 0.554915 | 4.1:+68346267 | None:intergenic |
| AGGTCGTCTCCACATTTCAA+TGG | 0.556393 | 4.1:-68352285 | MS.gene067697:intron |
| AAGTACTGAGATGATGGAAG+AGG | 0.558158 | 4.1:-68343772 | MS.gene067697:CDS |
| TTACCAAGGCGAAATTTGAA+AGG | 0.559040 | 4.1:+68343443 | None:intergenic |
| GATGAATACTCCGAGGGCCA+AGG | 0.559789 | 4.1:+68351023 | None:intergenic |
| GCAGGTGTTGAAATCAAACC+TGG | 0.561306 | 4.1:-68352341 | MS.gene067697:intron |
| ATACTACCTTCTTCACAGGT+TGG | 0.562544 | 4.1:-68347061 | MS.gene067697:CDS |
| AGGCAGGAAAGCTGAAACCA+CGG | 0.563166 | 4.1:-68347089 | MS.gene067697:intron |
| GGAACTGCCAACTGGTCTGA+AGG | 0.564194 | 4.1:+68348639 | None:intergenic |
| CCTTCTTCACAGGTTGGTCA+AGG | 0.564207 | 4.1:-68347055 | MS.gene067697:CDS |
| GTGCGAAAGGCAGCAGAAGA+CGG | 0.564863 | 4.1:-68349973 | MS.gene067697:CDS |
| TTGCAGATAGTCAGACTCAC+AGG | 0.565785 | 4.1:-68349240 | MS.gene067697:intron |
| TGCATGTTTCAGGTCTAAGA+AGG | 0.568255 | 4.1:-68343054 | MS.gene067697:intron |
| TGCAGCATTATCTAGTTCAG+TGG | 0.568551 | 4.1:+68349414 | None:intergenic |
| AAGGCAGCAGAAGACGGCTG+AGG | 0.570683 | 4.1:-68349967 | MS.gene067697:CDS |
| AAGCACTCTGCAATGTAATG+TGG | 0.570727 | 4.1:-68351149 | MS.gene067697:CDS |
| ATGGGAAAATTGCTACAGTA+GGG | 0.572946 | 4.1:-68343659 | MS.gene067697:CDS |
| AAGCTCCAAATTCAATTGCA+AGG | 0.573154 | 4.1:+68351075 | None:intergenic |
| CTGATCCAAGACCCTTACAA+AGG | 0.575653 | 4.1:+68349802 | None:intergenic |
| AGCACTCTGCAATGTAATGT+GGG | 0.576664 | 4.1:-68351148 | MS.gene067697:CDS |
| AAGAAGAAAAGTAAAAGTCA+GGG | 0.578925 | 4.1:-68343837 | MS.gene067697:CDS |
| CGCAGCATTATCTAGATCGG+TGG | 0.581023 | 4.1:+68348524 | None:intergenic |
| GGAACTGTCAACTGATCTGA+AGG | 0.581399 | 4.1:+68349508 | None:intergenic |
| GGATCGACCAAGTGATCAGA+AGG | 0.590673 | 4.1:+68349095 | None:intergenic |
| GACAAAGAAGCAGTTGATGA+TGG | 0.591027 | 4.1:-68348894 | MS.gene067697:CDS |
| GAAGAATAAATCCTTTGTAA+GGG | 0.591352 | 4.1:-68349813 | MS.gene067697:CDS |
| CCAACATCCGGAACAGTGCA+AGG | 0.592707 | 4.1:+68349074 | None:intergenic |
| TAAACCAACCATGAATTTGG+TGG | 0.597643 | 4.1:+68343011 | None:intergenic |
| CCAACATCCGGAACAGTGGA+AGG | 0.599169 | 4.1:+68348618 | None:intergenic |
| CCAACATCCGGAACAGTGGA+AGG | 0.599169 | 4.1:+68349487 | None:intergenic |
| AGCATTATCTAGTTCAGTGG+AGG | 0.600926 | 4.1:+68349417 | None:intergenic |
| GAAACCAAGAGTTCTAGTAA+TGG | 0.601240 | 4.1:-68349008 | MS.gene067697:CDS |
| AGTATTGCCATAGCGGCCAC+GGG | 0.602067 | 4.1:+68350982 | None:intergenic |
| TTGCGCAGCATTATCTAGAT+CGG | 0.602410 | 4.1:+68348521 | None:intergenic |
| AATGTTGTTAACCTGCCTCA+GGG | 0.602636 | 4.1:-68346953 | MS.gene067697:CDS |
| GCAGAGGAAGCATCTTCTGA+TGG | 0.602658 | 4.1:-68350007 | MS.gene067697:intron |
| TGCGGTTATTATCTTGCCCG+TGG | 0.602837 | 4.1:-68350998 | MS.gene067697:CDS |
| TGCAGTGCCATGTCCCAAAG+TGG | 0.602865 | 4.1:+68351180 | None:intergenic |
| GGTGCTCAAGACAATCCTGA+TGG | 0.604417 | 4.1:-68343894 | MS.gene067697:CDS |
| GTGGTGATTGAATCAAATGC+TGG | 0.607008 | 4.1:-68343473 | MS.gene067697:CDS |
| TTCCTGATCAAAGTGATACT+GGG | 0.607823 | 4.1:+68349366 | None:intergenic |
| AACAAAGCTTCACATCCTGA+AGG | 0.609738 | 4.1:-68346210 | MS.gene067697:CDS |
| ATCACATACGTCATTGATGG+TGG | 0.611631 | 4.1:+68343161 | None:intergenic |
| ATCCCCAACATCCGGAACAG+TGG | 0.617458 | 4.1:+68348614 | None:intergenic |
| CAGATCAGCATCAATTACAC+TGG | 0.623336 | 4.1:-68343515 | MS.gene067697:CDS |
| AAACTTATCACTCAGACCAT+CGG | 0.623766 | 4.1:+68346125 | None:intergenic |
| TTCCTGATCCAAGAGATCCT+GGG | 0.623794 | 4.1:+68348932 | None:intergenic |
| ACAGACGAGGATCTTCTGGC+AGG | 0.625586 | 4.1:-68348480 | MS.gene067697:CDS |
| GAAAGATTACCATTGAAATG+TGG | 0.627543 | 4.1:+68352276 | None:intergenic |
| CGCAGAGATGAATACTCCGA+GGG | 0.629223 | 4.1:+68351017 | None:intergenic |
| CATCCATAGTATTGCCATAG+CGG | 0.629804 | 4.1:+68350975 | None:intergenic |
| ATTCTCAAAAGAAAACAAAG+AGG | 0.632744 | 4.1:-68346251 | MS.gene067697:CDS |
| GGTGACAACAGAAATGGAGA+AGG | 0.633425 | 4.1:-68346189 | MS.gene067697:CDS |
| AATACTCCGAGGGCCAAGGA+CGG | 0.634343 | 4.1:+68351027 | None:intergenic |
| ATCCCCAACATCCGGAACAG+TGG | 0.646276 | 4.1:+68349483 | None:intergenic |
| TGGTGCAGCAGTGTGGACAA+TGG | 0.653645 | 4.1:-68343915 | MS.gene067697:intron |
| AAAGAGACAGCAGAGTCCGA+TGG | 0.654236 | 4.1:-68346141 | MS.gene067697:CDS |
| GAGGAAGCATCTTCTGATGG+CGG | 0.656231 | 4.1:-68350004 | MS.gene067697:CDS |
| TGTTTCAGGTCTAAGAAGGA+CGG | 0.657592 | 4.1:-68343050 | MS.gene067697:intron |
| TTATTCAAGAGCTTGAGACA+GGG | 0.658642 | 4.1:-68343689 | MS.gene067697:CDS |
| GTAGAGTCGGATGATGATAG+CGG | 0.678545 | 4.1:-68349929 | MS.gene067697:CDS |
| GCTGAGGAAGAAGTACCACT+TGG | 0.681517 | 4.1:-68349951 | MS.gene067697:CDS |
| TTACTGTGTTCATTTCCCTG+AGG | 0.689976 | 4.1:+68346938 | None:intergenic |
| CATCATCCGACTCTACCAAG+TGG | 0.700404 | 4.1:+68349936 | None:intergenic |
| CCGCAGAGATGAATACTCCG+AGG | 0.717928 | 4.1:+68351016 | None:intergenic |
| CAAGTTGAAGGAAAACGGGG+TGG | 0.725155 | 4.1:-68343492 | MS.gene067697:CDS |
| CCTCGGAGTATTCATCTCTG+CGG | 0.742424 | 4.1:-68351016 | MS.gene067697:CDS |
| TGGCAAGTTGAAGGAAAACG+GGG | 0.750008 | 4.1:-68343495 | MS.gene067697:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 68342983 | 68352453 | 68342983 | ID=MS.gene067697 |
| chr4.1 | mRNA | 68342983 | 68352453 | 68342983 | ID=MS.gene067697.t1;Parent=MS.gene067697 |
| chr4.1 | exon | 68352441 | 68352453 | 68352441 | ID=MS.gene067697.t1.exon1;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68352441 | 68352453 | 68352441 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68352286 | 68352359 | 68352286 | ID=MS.gene067697.t1.exon2;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68352286 | 68352359 | 68352286 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68350971 | 68351203 | 68350971 | ID=MS.gene067697.t1.exon3;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68350971 | 68351203 | 68350971 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68350123 | 68350259 | 68350123 | ID=MS.gene067697.t1.exon4;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68350123 | 68350259 | 68350123 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68349674 | 68350025 | 68349674 | ID=MS.gene067697.t1.exon5;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68349674 | 68350025 | 68349674 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68349241 | 68349540 | 68349241 | ID=MS.gene067697.t1.exon6;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68349241 | 68349540 | 68349241 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68348807 | 68349130 | 68348807 | ID=MS.gene067697.t1.exon7;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68348807 | 68349130 | 68348807 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68348447 | 68348671 | 68348447 | ID=MS.gene067697.t1.exon8;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68348447 | 68348671 | 68348447 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68346920 | 68347105 | 68346920 | ID=MS.gene067697.t1.exon9;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68346920 | 68347105 | 68346920 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68346084 | 68346323 | 68346084 | ID=MS.gene067697.t1.exon10;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68346084 | 68346323 | 68346084 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68343653 | 68343926 | 68343653 | ID=MS.gene067697.t1.exon11;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68343653 | 68343926 | 68343653 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68343447 | 68343534 | 68343447 | ID=MS.gene067697.t1.exon12;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68343447 | 68343534 | 68343447 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68343318 | 68343359 | 68343318 | ID=MS.gene067697.t1.exon13;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68343318 | 68343359 | 68343318 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68343170 | 68343218 | 68343170 | ID=MS.gene067697.t1.exon14;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68343170 | 68343218 | 68343170 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| chr4.1 | exon | 68342983 | 68343064 | 68342983 | ID=MS.gene067697.t1.exon15;Parent=MS.gene067697.t1 |
| chr4.1 | CDS | 68342983 | 68343064 | 68342983 | ID=cds.MS.gene067697.t1;Parent=MS.gene067697.t1 |
| Gene Sequence |
| Protein sequence |
