AlfalfaGEDB | MS.gene29304

M. sativa cultivar XinJiangDaYe / MS.gene29304

KEGG: K03444
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00083.27

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29304.t1	XP_003626331.2	97.7	483	9	1	1	483	1	481	2.30E-256	894.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29304.t1	Q3ECP7	68.0	450	144	0	11	460	16	465	4.8e-170	599.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29304.t1	A2Q5Z1	97.7	483	9	1	1	483	1	481	1.6e-256	894.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060485	MS.gene29304	0.811691	6.17E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene022492	MS.gene29304	PPI
MS.gene25807	MS.gene29304	PPI
MS.gene27308	MS.gene29304	PPI
MS.gene29304	MS.gene017905	PPI
MS.gene29304	MS.gene031757	PPI
MS.gene29304	MS.gene00961	PPI
MS.gene29304	MS.gene006416	PPI
MS.gene29304	MS.gene52364	PPI
MS.gene29304	MS.gene004491	PPI
MS.gene29304	MS.gene036548	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29304.t1	MTR_7g113960	97.723	483	9	1	1	483	1	481	0.0	941
MS.gene29304.t1	MTR_7g113970	77.727	440	98	0	22	461	57	496	0.0	719
MS.gene29304.t1	MTR_7g113960	97.548	367	7	1	1	367	1	365	0.0	706
MS.gene29304.t1	MTR_7g113970	74.722	360	84	1	22	374	57	416	0.0	554
MS.gene29304.t1	MTR_4g118610	54.605	456	204	2	7	461	34	487	2.91e-178	509
MS.gene29304.t1	MTR_2g020710	54.233	437	199	1	26	461	48	484	4.11e-171	491
MS.gene29304.t1	MTR_7g094450	51.835	436	210	0	26	461	39	474	1.16e-157	456
MS.gene29304.t1	MTR_7g094110	53.349	433	202	0	29	461	44	476	3.51e-157	455
MS.gene29304.t1	MTR_2g020710	54.684	395	178	1	68	461	1	395	6.56e-155	446
MS.gene29304.t1	MTR_7g094110	49.769	432	197	1	30	461	45	456	4.80e-139	408

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29304.t1	AT1G54730	67.550	453	147	0	9	461	14	466	0.0	612
MS.gene29304.t1	AT1G54730	63.355	453	145	1	9	461	14	445	0.0	560
MS.gene29304.t1	AT1G54730	67.221	421	138	0	9	429	14	434	0.0	558
MS.gene29304.t1	AT1G54730	62.708	421	136	1	9	429	14	413	4.09e-178	506
MS.gene29304.t1	AT1G54730	66.841	383	127	0	9	391	14	396	3.09e-175	498
MS.gene29304.t1	AT1G54730	66.841	383	127	0	9	391	14	396	3.09e-175	498
MS.gene29304.t1	AT2G48020	52.597	462	217	1	3	462	2	463	1.45e-173	496
MS.gene29304.t1	AT2G48020	52.597	462	217	1	3	462	2	463	1.45e-173	496
MS.gene29304.t1	AT5G18840	53.468	447	207	1	16	461	33	479	2.55e-165	476
MS.gene29304.t1	AT1G08930	49.776	446	224	0	16	461	47	492	1.81e-156	454

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Find 119 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAGTACCAGAGACCA+AGG	0.643814	7.2:+3394041	MS.gene29304:CDS
AAAAGAATGACAGAGATACA+AGG	0.644048	7.2:-3393145	None:intergenic
AAACTAGTACCAGAGACCAA+GGG	0.649846	7.2:+3394042	MS.gene29304:CDS
AACTAGTACCAGAGACCAAG+GGG	0.634126	7.2:+3394043	MS.gene29304:CDS
AAGAACCAGAGACAGCAGCA+AGG	0.646758	7.2:-3390162	None:intergenic
AAGTATTGGAACAATAGCAA+TGG	0.641668	7.2:+3392805	MS.gene29304:CDS
AATACTAACAATTGGAGCGA+TGG	0.564654	7.2:+3390775	MS.gene29304:CDS
AATAGCAATGGTTGTTGTTC+AGG	0.339658	7.2:+3392817	MS.gene29304:CDS
AATCCACTTTACAGCACCTT+AGG	0.388303	7.2:+3392141	MS.gene29304:CDS
AATCTCCCTCGAAGATCCTT+GGG	0.474385	7.2:-3391394	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAATGACAGAGATACA+AGG	-	chr7.2:3393148-3393167	None:intergenic	30.0%
AAATTACTGAACAGTGTGAA+GGG	-	chr7.2:3392941-3392960	None:intergenic	30.0%
AATGGGTATTCTATCATATG+TGG	+	chr7.2:3391246-3391265	MS.gene29304:CDS	30.0%
AATTTGATGTTATAGGCCAT+GGG	+	chr7.2:3390927-3390946	MS.gene29304:intron	30.0%
ATAATACTACAACAGTTTGG+CGG	+	chr7.2:3392484-3392503	MS.gene29304:CDS	30.0%
ATAGGTAAAGATGTTCTTTC+TGG	+	chr7.2:3391660-3391679	MS.gene29304:intron	30.0%
ATATTTCCCATCAATGTGAA+GGG	+	chr7.2:3393729-3393748	MS.gene29304:CDS	30.0%
ATCCAACAAAGAGATGAATT+TGG	+	chr7.2:3394113-3394132	MS.gene29304:CDS	30.0%
ATGATTATCTAGTCAAGATG+AGG	-	chr7.2:3393697-3393716	None:intergenic	30.0%
ATTGTTTGTGCTAATTGTGT+TGG	+	chr7.2:3391844-3391863	MS.gene29304:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	3390070	3394167	3390070	ID=MS.gene29304
chr7.2	mRNA	3390070	3394167	3390070	ID=MS.gene29304.t1;Parent=MS.gene29304
chr7.2	exon	3390070	3390201	3390070	ID=MS.gene29304.t1.exon1;Parent=MS.gene29304.t1
chr7.2	CDS	3390070	3390201	3390070	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3390307	3390369	3390307	ID=MS.gene29304.t1.exon2;Parent=MS.gene29304.t1
chr7.2	CDS	3390307	3390369	3390307	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3390758	3390847	3390758	ID=MS.gene29304.t1.exon3;Parent=MS.gene29304.t1
chr7.2	CDS	3390758	3390847	3390758	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3390942	3391001	3390942	ID=MS.gene29304.t1.exon4;Parent=MS.gene29304.t1
chr7.2	CDS	3390942	3391001	3390942	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3391202	3391267	3391202	ID=MS.gene29304.t1.exon5;Parent=MS.gene29304.t1
chr7.2	CDS	3391202	3391267	3391202	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3391367	3391432	3391367	ID=MS.gene29304.t1.exon6;Parent=MS.gene29304.t1
chr7.2	CDS	3391367	3391432	3391367	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3391588	3391663	3391588	ID=MS.gene29304.t1.exon7;Parent=MS.gene29304.t1
chr7.2	CDS	3391588	3391663	3391588	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3391750	3391814	3391750	ID=MS.gene29304.t1.exon8;Parent=MS.gene29304.t1
chr7.2	CDS	3391750	3391814	3391750	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3392113	3392205	3392113	ID=MS.gene29304.t1.exon9;Parent=MS.gene29304.t1
chr7.2	CDS	3392113	3392205	3392113	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3392311	3392391	3392311	ID=MS.gene29304.t1.exon10;Parent=MS.gene29304.t1
chr7.2	CDS	3392311	3392391	3392311	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3392469	3392553	3392469	ID=MS.gene29304.t1.exon11;Parent=MS.gene29304.t1
chr7.2	CDS	3392469	3392553	3392469	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3392795	3392838	3392795	ID=MS.gene29304.t1.exon12;Parent=MS.gene29304.t1
chr7.2	CDS	3392795	3392838	3392795	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3392973	3393035	3392973	ID=MS.gene29304.t1.exon13;Parent=MS.gene29304.t1
chr7.2	CDS	3392973	3393035	3392973	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3393113	3393172	3393113	ID=MS.gene29304.t1.exon14;Parent=MS.gene29304.t1
chr7.2	CDS	3393113	3393172	3393113	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3393265	3393318	3393265	ID=MS.gene29304.t1.exon15;Parent=MS.gene29304.t1
chr7.2	CDS	3393265	3393318	3393265	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3393580	3393639	3393580	ID=MS.gene29304.t1.exon16;Parent=MS.gene29304.t1
chr7.2	CDS	3393580	3393639	3393580	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3393729	3393843	3393729	ID=MS.gene29304.t1.exon17;Parent=MS.gene29304.t1
chr7.2	CDS	3393729	3393843	3393729	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1
chr7.2	exon	3393989	3394167	3393989	ID=MS.gene29304.t1.exon18;Parent=MS.gene29304.t1
chr7.2	CDS	3393989	3394167	3393989	ID=cds.MS.gene29304.t1;Parent=MS.gene29304.t1

Gene Sequence

>MS.gene29304

ATGAAGACTAAGTTTAAAGATTTTTCTCGTCTACTTTCTTCCATTCGCGAAGAAGCTAGTTCGGTACCAACTAGTATTCTTATACTTACCACCCTTGCTGCTGTCTCTGGTTCTTATGTTTTTGGCTCTGCGGTAAGTTCTCATTTACATCATTTTTTGGAGGATTTAATTAACAAGTTTATGATATACTATATCAGAATTCAATTCAGAATGAAAACTATCACTCTCATCTTGCAGGTGGGCTATTCATCCCCTGCTCAATCTGGAATCACAGATGACCTTGATCTTGGCGTAGCCGAGGTTTGAACTTTCTTTGAATGTTTGAATCACATAAACAACTTATTTATCGATATTGTTGGCTTTAGTTGACTTGGAGTGGCTTTCTCTATGGCTTGTTTGGGTTGACTTATTTGAGTTTATCTATAGGCATAAACACTTATGTGCTGTCTGAAAGAGTTTATAGAAATATCTTATCAACATATTGATAAGTTGTTTTCTTCTATTGACTCTCCATGATAGCTTGTGAAAACAGCTTATAGTTCATATAAAAATAGTTTGACTTTATTTTATCTATTGTTATAGAAATAGCTTACATATAAACACTTATATGATAAACGTTTTTGCTACAAGCGCTTAATTAAGTTGTTTATCCAAACAGAACTAAGTCAAACTAGTTGATTTTATGCAGTATTCACTTTTTGGTTCAATACTAACAATTGGAGCGATGGTTGGTGCCATTGTAAGTGGTAGTTTAGCAGATTATGCAGGTCGTCGAGCCGTAAGTTTCTCCAATTCTGTCTATTTCAACTATAATTAATTAAAATTGTATGCTTTTTTGTCTTATTTATTGTTCTTTCAATTTGATGTTATAGGCCATGGGATTCTCCGAGCTATGTTGCATCTTGGGATGGCTTGCCATTGCAGTCTCAAAGGTAACTGTTTCTATGACTCCATTTCCATATTTAATAAATTTGAGTACATTAGGACCTATAATAAATGGAAGGTTGTAAATCAGTTCTTTATTGTAGTTTGAATTATTCCTCCGTGTTTCTTCAATTCTGTCCAGTTTTGGGAATTCTTTCTTATACCAACGTAGTTGCTATTTGATGGTTTGGTTGATTTTTGTTTGAAGGTTGCTTGGTGGCTTTATGTTGGAAGACTATTGTTAGGATGTGGAATGGGTATTCTATCATATGTGGTAATTCTTTTGATTCTTATTTTTTCATAATTTCAGTTTAAATGATACTCAGTCACTCCCAAGAAATGCAACAAATAGGCAGTTCTCTTCCCATTTTAGGTACCAATTTATATAGCTGAAATAACACCCAAGGATCTTCGAGGGAGATTCACTGCAGTTCATCAGGTAAATCAATTGCGTTAAATATTATAAAGGGTCAATAGTTTAAATTACGGTTGCGGTCAGAACTTTTATATTGATGTGGTCAGAAATGCGGTTATTATGCTGTTGCGGATACATGTAGGCTTACTTTAAATCTTTATTTGGTATGATCTTGACAGCTAATGATTTGTTTTGGGGTATCACTAACTTATCTTATTGGAGCATTCTTCAATTGGAGGCTTTTGGCTATAATAGGTAAAGATGTTCTTTCTGGTTTTATATTTACAAAATTAAATTAAAAAATCTTTGCATTAGCTCAGATTTTTATTTCTTTATTTCAGGAACTATTCCATGTCTTGCACAGCTTCTGAGTCTATCCTTTATTCCCGAGTCTCCTAGGTGGCTGGTGAGTTGTTCACAAGCTACTCTAGATTTATTGTTTGTGCTAATTGTGTTGGACACAATTCAAACAAGCAACTGAAATTTTTCATCATTTTTGTCAAATTGTAGGAGTCAACTTGTCATTAGACGAACTCAATCCACTGACACAAACACGGCACCGACATTGACACGTAAACACTGATAATGATTTGAAAAAATAGAGGTGATTGTATGTAATCACAAGTGTCTGTGTTGTGTCAGACACGCTTTCAATATAAAGTTTCAGTGCTACATTTACAAGTGATAGTTGCATTGTTCTGCAGGCAAAGGTTGGTCGCTTGGAAAGGAGTGAATCCACTTTACAGCACCTTAGGGGAAAGAATGTTGATATTTCTGAAGAGGCCACTGAAATTAGAGTATGTAATAAGAGTTTGTTCATTAGAGTGTTGACTTGTGGGTTATCATCTAGCTAGGAAGAAACCTAAAAGTGTAACATATTTTTATACACTTACTTTTTGTAGGAATTTACAGAAGCCTCTCAGCAGCAAACAGAAGCTAACATTTTTGGGTTATTTCAATTGCAGTATTTGAAGTCACTTACTGTGAGATTTCTAATCCCAATTCATGCATCTTATCTTAAAAATATGCAGATTCTTATGGTATTTAGTCACATTGTTAGGTAGGAGTTGGCCTGATAATACTACAACAGTTTGGCGGAGTTAACGGCATTGCTTTTTATGCAAGTTCTATATTTGTCTCTGCTGGTATGTGTACGTTTTATTTATGCTTCTATCATATTTAAAACTTCACATAGAAAACGTGTCAAATGTCGAATACCAGCAGAATTAATAATTCTATTTTCTCAAATTATTACTGGTTGACCATCAGTGACATGTCTGGTGTTGGTGCTTCATAGCATATGACATGTAAAGAAGGTGATTACAATAACTAACATTATATTTATTACATGATTCTCATGAAAGGGAAAATTCTTATCCATCACAGGGTTTTCCAGAAGTATTGGAACAATAGCAATGGTTGTTGTTCAGGTTCTTAGTTCTCTTCATATAATTTGTTTATTTTTGTCCTCTTAATATGATGAATAAGATGAGATAGGTGTCAATTTTGATGAGTCTTCATTACTGAGCCCCTTCACACTGTTCAGTAATTTCTGTCTTCATAGATCCCAATGACAGCTTTGGGAGTAATTTTGATGGATAAATCTGGAAGACGACCCCTTTTACTGGTAAAACGATTAAACTTTTGTGCCTTCCTTTTCATGCTATTCTGTAAATTCTAAATCTAAAATTTTACATTATACAGATCTCTGCATCAGGAACATGCTTAGGATGCTTCCTTGTATCTCTGTCATTCTTTTTGCAGGTTCTCTGAATAATAAGATGATCTGCTCTTGCTAGGTTGTTCTTCACAATAGGTATTTCTAAATCAACAGATTGTTTTCTTCCAATATGCAGGACTTGCATAAATGGAAGGAATTCAGTCCCATTCTGGCACTCGTCGGTGTACTGGTATTGATTTTGTCTGATTATGTTGCTTCTAACTCATTCCTACAAAACCGATTTATAAGTCGAGGAGTGTCATTATTTTATAAACTTTCTCTATTTTAAATGTGGAACTTGAGTTTTTCCTACTAATACTTTCTCTGATTTTTCACCAACCTTATATTTGCATTCATGAAATAGACTTGGTTTAAGAATACCGCGATGGTGGTAAAGTTAAAAGGTATATGGTAATATACATTTTCACCATTTAGTTACCATAATAATCAGGTATATACAGGATCTTTCTCACTTGGCATGGGAGGAATACCCTGGGTTATAATGTCTGAGGTAATATGCTCCTTCACTGGGTATGCAAATTTTGATCGCAAGTTCATAACAAGTCCTCATCTTGACTAGATAATCATTTTTTTTACCAGATATTTCCCATCAATGTGAAGGGTTCTGCTGGAAGCTTTGTTACATTTGTTCACTGGTTGTGTTCTTGGATTGTATCATATGCTTTTAACTTCCTCATGAGTTGGAATTCAGCAGGTAGGTTTTCATTTACTTTTGATCCCTCTAGAAGAAAACAAAAAATCGGAAAGGACAAACACAAAAATGAATGAAGTCTTGAAAAGTAGGGTATGCTTCTTAAGTGTGGAATAAGTTCATTAATTAATGTATTATTTTTTACCAGGAACTTTCTTCATATTCTCTAGCATCTGTGGCCTCACCATTCTATTTGTAGCAAAACTAGTACCAGAGACCAAGGGGCGTACGCTAGAAGAAGTTCAAGCGTCTCTCAATCCATATCAGACAGTATCCAACAAAGAGATGAATTTGGCATATGATCCTGTAACAGAATTGCATGCATAA

Protein sequence

>MS.gene29304.t1

MKTKFKDFSRLLSSIREEASSVPTSILILTTLAAVSGSYVFGSAVGYSSPAQSGITDDLDLGVAEYSLFGSILTIGAMVGAIVSGSLADYAGRRAAMGFSELCCILGWLAIAVSKVAWWLYVGRLLLGCGMGILSYVVPIYIAEITPKDLRGRFTAVHQLMICFGVSLTYLIGAFFNWRLLAIIGTIPCLAQLLSLSFIPESPRWLAKVGRLERSESTLQHLRGKNVDISEEATEIREFTEASQQQTEANIFGLFQLQYLKSLTVGVGLIILQQFGGVNGIAFYASSIFVSAGFSRSIGTIAMVVVQIPMTALGVILMDKSGRRPLLLISASGTCLGCFLVSLSFFLQDLHKWKEFSPILALVGVLVYTGSFSLGMGGIPWVIMSEIFPINVKGSAGSFVTFVHWLCSWIVSYAFNFLMSWNSAGTFFIFSSICGLTILFVAKLVPETKGRTLEEVQASLNPYQTVSNKEMNLAYDPVTELHA