AlfalfaGEDB | MS.gene50775

M. sativa cultivar XinJiangDaYe / MS.gene50775

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50775.t1	KEH30500.1	100	93	0	0	3	95	1	93	4.50E-47	196.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50775.t1	Q9ZNT3	86.0	93	13	0	3	95	1	93	3.5e-40	165.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50775.t1	A0A072UM89	100.0	93	0	0	3	95	1	93	3.2e-47	196.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene50775	0.812123	4.97E-51	-1.69E-46
MS.gene049159	MS.gene50775	0.823846	1.11E-53	-1.69E-46
MS.gene049255	MS.gene50775	0.827818	1.27E-54	-1.69E-46
MS.gene049341	MS.gene50775	0.819212	1.31E-52	-1.69E-46
MS.gene049404	MS.gene50775	0.80181	7.55E-49	-1.69E-46
MS.gene049653	MS.gene50775	0.803481	3.41E-49	-1.69E-46
MS.gene049808	MS.gene50775	0.836024	1.19E-56	-1.69E-46
MS.gene049821	MS.gene50775	0.818759	1.66E-52	-1.69E-46
MS.gene050277	MS.gene50775	0.802391	5.74E-49	-1.69E-46
MS.gene050559	MS.gene50775	0.803906	2.79E-49	-1.69E-46
MS.gene050561	MS.gene50775	0.801215	1.00E-48	-1.69E-46
MS.gene050689	MS.gene50775	0.82223	2.65E-53	-1.69E-46
MS.gene050807	MS.gene50775	0.834874	2.33E-56	-1.69E-46
MS.gene050901	MS.gene50775	0.809608	1.74E-50	-1.69E-46
MS.gene051243	MS.gene50775	0.808173	3.53E-50	-1.69E-46
MS.gene051247	MS.gene50775	0.814352	1.61E-51	-1.69E-46
MS.gene051279	MS.gene50775	0.804961	1.68E-49	-1.69E-46
MS.gene051328	MS.gene50775	0.844049	9.61E-59	-1.69E-46
MS.gene051343	MS.gene50775	0.805807	1.12E-49	-1.69E-46
MS.gene05134	MS.gene50775	0.845795	3.25E-59	-1.69E-46
MS.gene051744	MS.gene50775	0.806161	9.41E-50	-1.69E-46
MS.gene051975	MS.gene50775	0.80396	2.72E-49	-1.69E-46
MS.gene052239	MS.gene50775	-0.812336	4.46E-51	-1.69E-46
MS.gene052516	MS.gene50775	0.813014	3.17E-51	-1.69E-46
MS.gene052519	MS.gene50775	0.809796	1.58E-50	-1.69E-46
MS.gene052947	MS.gene50775	0.824718	6.93E-54	-1.69E-46
MS.gene053006	MS.gene50775	0.821968	3.04E-53	-1.69E-46
MS.gene053008	MS.gene50775	0.807117	5.91E-50	-1.69E-46
MS.gene053226	MS.gene50775	0.821959	3.06E-53	-1.69E-46
MS.gene053541	MS.gene50775	0.809263	2.06E-50	-1.69E-46
MS.gene053542	MS.gene50775	0.820038	8.47E-53	-1.69E-46
MS.gene053590	MS.gene50775	0.827748	1.32E-54	-1.69E-46
MS.gene05364	MS.gene50775	0.841278	5.24E-58	-1.69E-46
MS.gene053931	MS.gene50775	0.86928	3.48E-66	-1.69E-46
MS.gene054651	MS.gene50775	0.80492	1.71E-49	-1.69E-46
MS.gene054657	MS.gene50775	0.826779	2.25E-54	-1.69E-46
MS.gene05476	MS.gene50775	0.809458	1.87E-50	-1.69E-46
MS.gene05517	MS.gene50775	0.801756	7.75E-49	-1.69E-46
MS.gene055464	MS.gene50775	0.800084	1.70E-48	-1.69E-46
MS.gene055623	MS.gene50775	0.811781	5.90E-51	-1.69E-46
MS.gene055808	MS.gene50775	0.802118	6.53E-49	-1.69E-46
MS.gene055818	MS.gene50775	0.806828	6.80E-50	-1.69E-46
MS.gene055841	MS.gene50775	0.835437	1.68E-56	-1.69E-46
MS.gene056215	MS.gene50775	0.838301	3.12E-57	-1.69E-46
MS.gene056308	MS.gene50775	0.805484	1.31E-49	-1.69E-46
MS.gene056366	MS.gene50775	0.822566	2.21E-53	-1.69E-46
MS.gene056760	MS.gene50775	0.813386	2.63E-51	-1.69E-46
MS.gene057060	MS.gene50775	0.812218	4.74E-51	-1.69E-46
MS.gene057208	MS.gene50775	0.845971	2.91E-59	-1.69E-46
MS.gene057287	MS.gene50775	0.817654	2.95E-52	-1.69E-46
MS.gene057392	MS.gene50775	0.832483	9.21E-56	-1.69E-46
MS.gene057444	MS.gene50775	0.829263	5.68E-55	-1.69E-46
MS.gene057598	MS.gene50775	0.81297	3.24E-51	-1.69E-46
MS.gene057693	MS.gene50775	0.822518	2.27E-53	-1.69E-46
MS.gene057694	MS.gene50775	0.883711	3.56E-71	-1.69E-46
MS.gene057705	MS.gene50775	0.844327	8.10E-59	-1.69E-46
MS.gene057855	MS.gene50775	0.806555	7.77E-50	-1.69E-46
MS.gene05795	MS.gene50775	0.811849	5.70E-51	-1.69E-46
MS.gene058008	MS.gene50775	0.815426	9.30E-52	-1.69E-46
MS.gene058009	MS.gene50775	0.838109	3.50E-57	-1.69E-46
MS.gene058010	MS.gene50775	0.81563	8.38E-52	-1.69E-46
MS.gene058054	MS.gene50775	0.802761	4.81E-49	-1.69E-46
MS.gene058055	MS.gene50775	0.811612	6.42E-51	-1.69E-46
MS.gene058088	MS.gene50775	0.827886	1.22E-54	-1.69E-46
MS.gene05847	MS.gene50775	0.819125	1.37E-52	-1.69E-46
MS.gene058634	MS.gene50775	0.82143	4.05E-53	-1.69E-46
MS.gene058781	MS.gene50775	0.812876	3.40E-51	-1.69E-46
MS.gene058783	MS.gene50775	0.835001	2.16E-56	-1.69E-46
MS.gene058979	MS.gene50775	0.835134	2.00E-56	-1.69E-46
MS.gene059317	MS.gene50775	0.818356	2.05E-52	-1.69E-46
MS.gene05978	MS.gene50775	0.816636	4.99E-52	-1.69E-46
MS.gene059842	MS.gene50775	0.820835	5.56E-53	-1.69E-46
MS.gene060244	MS.gene50775	0.839126	1.91E-57	-1.69E-46
MS.gene060310	MS.gene50775	0.829455	5.10E-55	-1.69E-46
MS.gene060365	MS.gene50775	0.81588	7.37E-52	-1.69E-46
MS.gene060429	MS.gene50775	0.847104	1.43E-59	-1.69E-46
MS.gene060587	MS.gene50775	0.801326	9.49E-49	-1.69E-46
MS.gene060917	MS.gene50775	0.806177	9.34E-50	-1.69E-46
MS.gene060935	MS.gene50775	0.816445	5.51E-52	-1.69E-46
MS.gene061100	MS.gene50775	0.802713	4.92E-49	-1.69E-46
MS.gene061176	MS.gene50775	0.816393	5.66E-52	-1.69E-46
MS.gene061177	MS.gene50775	0.816103	6.57E-52	-1.69E-46
MS.gene061178	MS.gene50775	0.816047	6.76E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50775.t1	MTR_4g073590	100.000	93	0	0	3	95	1	93	2.35e-66	194
MS.gene50775.t1	MTR_4g073590	100.000	93	0	0	3	95	1	93	2.46e-66	196
MS.gene50775.t1	MTR_7g096890	70.930	86	25	0	10	95	11	96	2.01e-43	138
MS.gene50775.t1	MTR_1g068950	61.628	86	33	0	10	95	4	89	1.30e-36	120
MS.gene50775.t1	MTR_5g010430	60.465	86	32	1	10	95	6	89	4.45e-35	117
MS.gene50775.t1	MTR_1g076170	59.794	97	33	1	5	95	1	97	1.63e-34	115
MS.gene50775.t1	MTR_2g028670	53.488	86	40	0	10	95	4	89	8.53e-32	108
MS.gene50775.t1	MTR_8g088210	54.651	86	39	0	10	95	4	89	1.36e-31	108
MS.gene50775.t1	MTR_8g098470	55.814	86	36	1	10	95	4	87	4.67e-31	106
MS.gene50775.t1	MTR_1g101190	59.302	86	33	1	10	95	465	548	1.58e-30	112
MS.gene50775.t1	MTR_8g088210	54.878	82	37	0	14	95	1	82	1.32e-29	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50775.t1	AT2G16700	86.022	93	13	0	3	95	1	93	2.19e-54	166
MS.gene50775.t1	AT2G16700	86.022	93	13	0	3	95	1	93	2.96e-54	164
MS.gene50775.t1	AT4G34970	80.000	90	18	0	5	94	1	90	3.82e-52	160
MS.gene50775.t1	AT2G16700	84.146	82	13	0	14	95	1	82	5.65e-46	144
MS.gene50775.t1	AT2G16700	84.146	82	13	0	14	95	1	82	5.65e-46	144
MS.gene50775.t1	AT2G31200	62.791	86	32	0	10	95	11	96	2.19e-37	123
MS.gene50775.t1	AT4G00680	60.465	86	34	0	10	95	4	89	4.41e-37	122
MS.gene50775.t1	AT4G00680	60.465	86	34	0	10	95	14	99	1.09e-36	121
MS.gene50775.t1	AT5G59890	61.628	86	33	0	10	95	4	89	4.12e-36	119
MS.gene50775.t1	AT3G46010	59.302	86	35	0	10	95	15	100	8.96e-36	119
MS.gene50775.t1	AT3G46010	59.302	86	35	0	10	95	4	89	1.07e-35	118
MS.gene50775.t1	AT1G01750	58.140	86	36	0	10	95	4	89	3.31e-35	117
MS.gene50775.t1	AT1G01750	58.140	86	36	0	10	95	9	94	3.82e-35	117
MS.gene50775.t1	AT5G59890	62.195	82	31	0	14	95	1	82	4.91e-34	114
MS.gene50775.t1	AT5G59890	62.195	82	31	0	14	95	1	82	4.91e-34	114
MS.gene50775.t1	AT5G59880	54.651	86	39	0	10	95	4	89	8.19e-34	113
MS.gene50775.t1	AT5G59880	54.651	86	39	0	10	95	4	89	1.00e-33	113
MS.gene50775.t1	AT4G25590	59.302	86	33	1	10	95	4	87	8.16e-33	111
MS.gene50775.t1	AT3G46000	60.465	86	32	1	10	95	4	87	1.71e-32	110
MS.gene50775.t1	AT3G46000	60.465	86	32	1	10	95	23	106	2.18e-32	110
MS.gene50775.t1	AT4G25590	59.756	82	31	1	14	95	1	80	9.73e-31	105
MS.gene50775.t1	AT3G46000	60.976	82	30	1	14	95	1	80	1.61e-30	105
MS.gene50775.t1	AT5G52360	55.814	86	36	1	10	95	4	87	1.66e-30	105
MS.gene50775.t1	AT3G45990	37.931	87	45	3	14	95	1	83	1.20e-12	59.7

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGGCGATGGCTTTCAAAA+TGG	0.335809	4.1:+30233539	MS.gene50775:CDS
GTTGACAAGGTTGGTGGTCC+TGG	0.380021	4.1:+30233835	MS.gene50775:CDS
GAATCTGAACAGGCTACTAC+TGG	0.427593	4.1:+30233706	MS.gene50775:intron
TGAGTGCAAGAACTCTTTCA+TGG	0.435498	4.1:+30233744	MS.gene50775:CDS
GCATATCTACAATCATCATT+AGG	0.474554	4.1:-30233889	None:intergenic
AACAGGCTACTACTGGGATG+TGG	0.527418	4.1:+30233713	MS.gene50775:intron
ACTCTTTCATGGAGATGAAG+TGG	0.542716	4.1:+30233755	MS.gene50775:CDS
CATGGAGATGAAGTGGAAGA+AGG	0.543453	4.1:+30233762	MS.gene50775:CDS
GCAAGATCTTCTTCATTGCA+TGG	0.552818	4.1:+30233956	MS.gene50775:CDS
TGAAGAAGATCTTGCTCTTG+CGG	0.553047	4.1:-30233948	None:intergenic
GTGACCGTTGACAAGGTTGG+TGG	0.590709	4.1:+30233829	MS.gene50775:CDS
TGAAGTGGAAGAAGGTGCAT+AGG	0.607407	4.1:+30233770	MS.gene50775:CDS
AAGGCTTGTGACCGTTGACA+AGG	0.611864	4.1:+30233822	MS.gene50775:CDS
ACAGGCTACTACTGGGATGT+GGG	0.613046	4.1:+30233714	MS.gene50775:intron
TTCTTCTCATCAGATAACAA+TGG	0.614668	4.1:+30233521	None:intergenic
TCATCAGATAACAATGGCGA+TGG	0.623350	4.1:+30233527	None:intergenic
AATCTGAACAGGCTACTACT+GGG	0.632062	4.1:+30233707	MS.gene50775:intron
AGGACCACCAACCTTGTCAA+CGG	0.633960	4.1:-30233833	None:intergenic
CTTGTGACCGTTGACAAGGT+TGG	0.653631	4.1:+30233826	MS.gene50775:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGTTTATACACATGTTTA+TGG	+	chr4.1:30233653-30233672	MS.gene50775:intron	20.0%
!	CATATAATATGAATCTGAAC+AGG	+	chr4.1:30233696-30233715	MS.gene50775:intron	25.0%
!	TATGAGTGAGATGATTTAAA+AGG	-	chr4.1:30233575-30233594	None:intergenic	25.0%
	GCATATCTACAATCATCATT+AGG	-	chr4.1:30233892-30233911	None:intergenic	30.0%
	TCAAGATCGATGAAAAAACA+AGG	+	chr4.1:30233803-30233822	MS.gene50775:CDS	30.0%
!	AGATCATCATAGTTTTCTCC+AGG	-	chr4.1:30233856-30233875	None:intergenic	35.0%
	AATCTGAACAGGCTACTACT+GGG	+	chr4.1:30233707-30233726	MS.gene50775:intron	40.0%
	AATGGCGATGGCTTTCAAAA+TGG	+	chr4.1:30233539-30233558	MS.gene50775:CDS	40.0%
	ACTCTTTCATGGAGATGAAG+TGG	+	chr4.1:30233755-30233774	MS.gene50775:CDS	40.0%
	TGAAGAAGATCTTGCTCTTG+CGG	-	chr4.1:30233951-30233970	None:intergenic	40.0%
	TGAGTGCAAGAACTCTTTCA+TGG	+	chr4.1:30233744-30233763	MS.gene50775:CDS	40.0%
	CATGGAGATGAAGTGGAAGA+AGG	+	chr4.1:30233762-30233781	MS.gene50775:CDS	45.0%
	GAATCTGAACAGGCTACTAC+TGG	+	chr4.1:30233706-30233725	MS.gene50775:intron	45.0%
!	TGAAGTGGAAGAAGGTGCAT+AGG	+	chr4.1:30233770-30233789	MS.gene50775:CDS	45.0%
	AACAGGCTACTACTGGGATG+TGG	+	chr4.1:30233713-30233732	MS.gene50775:intron	50.0%
	AAGGCTTGTGACCGTTGACA+AGG	+	chr4.1:30233822-30233841	MS.gene50775:CDS	50.0%
	ACAGGCTACTACTGGGATGT+GGG	+	chr4.1:30233714-30233733	MS.gene50775:intron	50.0%
	AGGACCACCAACCTTGTCAA+CGG	-	chr4.1:30233836-30233855	None:intergenic	50.0%
	CTTGTGACCGTTGACAAGGT+TGG	+	chr4.1:30233826-30233845	MS.gene50775:CDS	50.0%
	GTGACCGTTGACAAGGTTGG+TGG	+	chr4.1:30233829-30233848	MS.gene50775:CDS	55.0%
!!	GTTGACAAGGTTGGTGGTCC+TGG	+	chr4.1:30233835-30233854	MS.gene50775:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	30233534	30233977	30233534	ID=MS.gene50775
chr4.1	mRNA	30233534	30233977	30233534	ID=MS.gene50775.t1;Parent=MS.gene50775
chr4.1	exon	30233534	30233560	30233534	ID=MS.gene50775.t1.exon1;Parent=MS.gene50775.t1
chr4.1	CDS	30233534	30233560	30233534	ID=cds.MS.gene50775.t1;Parent=MS.gene50775.t1
chr4.1	exon	30233718	30233977	30233718	ID=MS.gene50775.t1.exon2;Parent=MS.gene50775.t1
chr4.1	CDS	30233718	30233977	30233718	ID=cds.MS.gene50775.t1;Parent=MS.gene50775.t1

Gene Sequence

>MS.gene50775

ATAACAATGGCGATGGCTTTCAAAATGGTACTTCTTTACCTTTTAAATCATCTCACTCATATTAGAGTGTGTGTGATTTTACTTTGTTCTATATATTATTATATATTGATGAATTTTGCTTTGTTTATACACATGTTTATGGTAGATAAAATTATGAATTAACATATAATATGAATCTGAACAGGCTACTACTGGGATGTGGGTAACAGATGAGTGCAAGAACTCTTTCATGGAGATGAAGTGGAAGAAGGTGCATAGGTACATAGTTTTCAAGATCGATGAAAAAACAAGGCTTGTGACCGTTGACAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAATGATGATTGTAGATATGCTGTCTTTGATTTTGATTTTGTCACTGTTGACAACTGCCGCAAGAGCAAGATCTTCTTCATTGCATG

Protein sequence

>MS.gene50775.t1

ITMAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEKTRLVTVDKVGGPGENYDDLAASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIA