Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66437.t1 | AES59361.2 | 90.2 | 399 | 34 | 1 | 1 | 399 | 1 | 394 | 3.50E-218 | 767.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66437.t1 | Q9STX2 | 66.4 | 399 | 123 | 2 | 1 | 399 | 1 | 388 | 6.3e-160 | 565.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66437.t1 | G7ICG0 | 90.2 | 399 | 34 | 1 | 1 | 399 | 1 | 394 | 2.5e-218 | 767.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene66437 | 0.808864 | 2.51E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene66437 | 0.831334 | 1.77E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene66437 | 0.829657 | 4.55E-55 | -1.69E-46 |
| MS.gene049232 | MS.gene66437 | 0.81988 | 9.21E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene66437 | 0.841556 | 4.42E-58 | -1.69E-46 |
| MS.gene049369 | MS.gene66437 | 0.805034 | 1.62E-49 | -1.69E-46 |
| MS.gene049396 | MS.gene66437 | 0.804409 | 2.19E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene66437 | 0.816754 | 4.70E-52 | -1.69E-46 |
| MS.gene049633 | MS.gene66437 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene66437 | 0.808343 | 3.25E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene66437 | 0.805644 | 1.21E-49 | -1.69E-46 |
| MS.gene050323 | MS.gene66437 | 0.853849 | 1.82E-61 | -1.69E-46 |
| MS.gene050504 | MS.gene66437 | 0.816212 | 6.21E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene66437 | 0.817265 | 3.61E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene66437 | 0.805446 | 1.33E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene66437 | 0.819509 | 1.12E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene66437 | 0.836903 | 7.12E-57 | -1.69E-46 |
| MS.gene050868 | MS.gene66437 | 0.833124 | 6.39E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene66437 | 0.837395 | 5.33E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene66437 | 0.814798 | 1.28E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene66437 | 0.808177 | 3.52E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene66437 | 0.804988 | 1.66E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene66437 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene051734 | MS.gene66437 | 0.823309 | 1.48E-53 | -1.69E-46 |
| MS.gene051858 | MS.gene66437 | 0.818427 | 1.97E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene66437 | 0.804878 | 1.75E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene66437 | 0.817493 | 3.20E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene66437 | 0.808943 | 2.42E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene66437 | 0.805262 | 1.45E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene66437 | 0.802737 | 4.87E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene66437 | 0.805268 | 1.45E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene66437 | 0.812988 | 3.21E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene66437 | 0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene052870 | MS.gene66437 | 0.8365 | 9.02E-57 | -1.69E-46 |
| MS.gene052872 | MS.gene66437 | 0.841642 | 4.20E-58 | -1.69E-46 |
| MS.gene052916 | MS.gene66437 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene053018 | MS.gene66437 | 0.807052 | 6.10E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene66437 | 0.822378 | 2.45E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene66437 | 0.820937 | 5.27E-53 | -1.69E-46 |
| MS.gene053286 | MS.gene66437 | 0.818578 | 1.82E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene66437 | 0.806603 | 7.59E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene66437 | 0.834839 | 2.38E-56 | -1.69E-46 |
| MS.gene053633 | MS.gene66437 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene053824 | MS.gene66437 | 0.845001 | 5.33E-59 | -1.69E-46 |
| MS.gene053916 | MS.gene66437 | 0.814326 | 1.63E-51 | -1.69E-46 |
| MS.gene054003 | MS.gene66437 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene66437 | 0.82532 | 5.00E-54 | -1.69E-46 |
| MS.gene054642 | MS.gene66437 | 0.818897 | 1.54E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene66437 | 0.806741 | 7.10E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene66437 | 0.824692 | 7.03E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene66437 | 0.848642 | 5.38E-60 | -1.69E-46 |
| MS.gene055206 | MS.gene66437 | 0.823281 | 1.51E-53 | -1.69E-46 |
| MS.gene055224 | MS.gene66437 | 0.816756 | 4.69E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene66437 | 0.814666 | 1.37E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene66437 | 0.831714 | 1.43E-55 | -1.69E-46 |
| MS.gene055722 | MS.gene66437 | 0.839062 | 1.98E-57 | -1.69E-46 |
| MS.gene055750 | MS.gene66437 | 0.803142 | 4.01E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene66437 | 0.813896 | 2.03E-51 | -1.69E-46 |
| MS.gene055941 | MS.gene66437 | 0.855412 | 6.42E-62 | -1.69E-46 |
| MS.gene056374 | MS.gene66437 | 0.807084 | 6.01E-50 | -1.69E-46 |
| MS.gene056448 | MS.gene66437 | 0.814682 | 1.36E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene66437 | 0.817079 | 3.97E-52 | -1.69E-46 |
| MS.gene056510 | MS.gene66437 | 0.819439 | 1.16E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene66437 | 0.80439 | 2.21E-49 | -1.69E-46 |
| MS.gene056549 | MS.gene66437 | 0.817158 | 3.81E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene66437 | 0.821031 | 5.01E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene66437 | 0.805085 | 1.58E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene66437 | 0.800308 | 1.53E-48 | -1.69E-46 |
| MS.gene057331 | MS.gene66437 | 0.807732 | 4.38E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene66437 | 0.836893 | 7.17E-57 | -1.69E-46 |
| MS.gene057856 | MS.gene66437 | 0.806218 | 9.15E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene66437 | 0.807085 | 6.00E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene66437 | 0.84177 | 3.89E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene66437 | 0.808853 | 2.53E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene66437 | 0.808731 | 2.68E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene66437 | 0.83606 | 1.17E-56 | -1.69E-46 |
| MS.gene05837 | MS.gene66437 | 0.801584 | 8.41E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene66437 | 0.813749 | 2.19E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene66437 | 0.803054 | 4.19E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene66437 | 0.805463 | 1.32E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene66437 | 0.823005 | 1.75E-53 | -1.69E-46 |
| MS.gene05913 | MS.gene66437 | 0.824597 | 7.40E-54 | -1.69E-46 |
| MS.gene059305 | MS.gene66437 | 0.816537 | 5.25E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene66437 | 0.84689 | 1.63E-59 | -1.69E-46 |
| MS.gene059367 | MS.gene66437 | 0.811119 | 8.21E-51 | -1.69E-46 |
| MS.gene059674 | MS.gene66437 | 0.802679 | 5.00E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene66437 | 0.803427 | 3.51E-49 | -1.69E-46 |
| MS.gene059680 | MS.gene66437 | 0.80333 | 3.67E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene66437 | 0.821344 | 4.25E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene66437 | 0.810692 | 1.02E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene66437 | 0.813988 | 1.94E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene66437 | 0.806895 | 6.59E-50 | -1.69E-46 |
| MS.gene060193 | MS.gene66437 | 0.804385 | 2.22E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene66437 | 0.845711 | 3.42E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene66437 | 0.804911 | 1.72E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene66437 | 0.812447 | 4.22E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene66437 | 0.839853 | 1.24E-57 | -1.69E-46 |
| MS.gene060835 | MS.gene66437 | 0.814216 | 1.72E-51 | -1.69E-46 |
| MS.gene060837 | MS.gene66437 | 0.828787 | 7.40E-55 | -1.69E-46 |
| MS.gene060982 | MS.gene66437 | 0.803578 | 3.26E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene66437 | 0.814609 | 1.41E-51 | -1.69E-46 |
| MS.gene061134 | MS.gene66437 | 0.809133 | 2.20E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene66437 | 0.806521 | 7.90E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene66437 | 0.804744 | 1.86E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66437.t1 | MTR_1g018590 | 90.226 | 399 | 34 | 1 | 1 | 399 | 1 | 394 | 0.0 | 754 |
| MS.gene66437.t1 | MTR_1g018610 | 85.714 | 399 | 48 | 1 | 1 | 399 | 34 | 423 | 0.0 | 717 |
| MS.gene66437.t1 | MTR_1g018580 | 85.464 | 399 | 23 | 2 | 1 | 399 | 1 | 364 | 0.0 | 700 |
| MS.gene66437.t1 | MTR_3g102760 | 70.000 | 400 | 109 | 2 | 1 | 399 | 1 | 390 | 0.0 | 592 |
| MS.gene66437.t1 | MTR_3g102770 | 67.419 | 399 | 120 | 1 | 1 | 399 | 1 | 389 | 0.0 | 570 |
| MS.gene66437.t1 | MTR_3g013890 | 67.500 | 400 | 119 | 2 | 1 | 399 | 1 | 390 | 0.0 | 564 |
| MS.gene66437.t1 | MTR_3g102730 | 67.500 | 400 | 119 | 2 | 1 | 399 | 1 | 390 | 0.0 | 563 |
| MS.gene66437.t1 | MTR_4g073090 | 40.811 | 370 | 209 | 6 | 37 | 399 | 6 | 372 | 1.94e-92 | 283 |
| MS.gene66437.t1 | MTR_4g121590 | 40.270 | 370 | 209 | 7 | 38 | 399 | 7 | 372 | 3.09e-89 | 275 |
| MS.gene66437.t1 | MTR_4g121570 | 39.837 | 369 | 212 | 7 | 38 | 399 | 7 | 372 | 1.61e-88 | 273 |
| MS.gene66437.t1 | MTR_3g463660 | 37.533 | 381 | 220 | 10 | 30 | 399 | 2 | 375 | 2.47e-77 | 244 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66437.t1 | AT4G24220 | 66.416 | 399 | 123 | 2 | 1 | 399 | 1 | 388 | 0.0 | 551 |
| MS.gene66437.t1 | AT4G24220 | 66.416 | 399 | 122 | 2 | 1 | 399 | 1 | 387 | 0.0 | 549 |
| MS.gene66437.t1 | AT5G58750 | 36.483 | 381 | 214 | 14 | 36 | 399 | 8 | 377 | 6.13e-74 | 235 |
| MS.gene66437.t1 | AT5G58750 | 36.483 | 381 | 214 | 14 | 36 | 399 | 16 | 385 | 7.53e-74 | 235 |
Find 94 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATAGCCGAACAACATATT+TGG | 0.126207 | 1.4:+4650434 | MS.gene66437:CDS |
| GCTAAAGAACATGGTTTCTT+AGG | 0.179575 | 1.4:+4650748 | MS.gene66437:CDS |
| AACATGGTTTCTTAGGATTT+AGG | 0.218287 | 1.4:+4650755 | MS.gene66437:CDS |
| AGCATGAGGGTCTTCCTTTA+AGG | 0.227760 | 1.4:+4650356 | MS.gene66437:CDS |
| CTCTTGCGTGTGATAGAAAT+TGG | 0.233398 | 1.4:-4650057 | None:intergenic |
| CGGTTTGAGATCGATGATTA+TGG | 0.235119 | 1.4:+4650550 | MS.gene66437:CDS |
| TTTGAGCCAGGAAACCTTAA+AGG | 0.244750 | 1.4:-4650370 | None:intergenic |
| GGTCTTCCTTTAAGGTTTCC+TGG | 0.265961 | 1.4:+4650364 | MS.gene66437:CDS |
| CAAAATGTAGCATTGATTAT+AGG | 0.274320 | 1.4:+4649606 | MS.gene66437:CDS |
| GGTGGTCCATGGAAGGTTTA+TGG | 0.276380 | 1.4:+4649687 | MS.gene66437:CDS |
| CTATAATCAATGCTACATTT+TGG | 0.276772 | 1.4:-4649605 | None:intergenic |
| AATAGCCGAACAACATATTT+GGG | 0.280052 | 1.4:+4650435 | MS.gene66437:CDS |
| AATCGACCACAAGTTATCTT+TGG | 0.308724 | 1.4:+4650271 | MS.gene66437:CDS |
| AATGCTTCATTCTTTGCATT+AGG | 0.329181 | 1.4:-4650469 | None:intergenic |
| TTTGGATCCTTCGAAATAAT+CGG | 0.345066 | 1.4:+4649978 | MS.gene66437:CDS |
| TGACAAAGATTCAGAGCATT+TGG | 0.349664 | 1.4:-4649924 | None:intergenic |
| AATATGTGTCACATCAGTTA+AGG | 0.393193 | 1.4:-4649811 | None:intergenic |
| CTATGAATTCATTCATAAGT+TGG | 0.400929 | 1.4:+4650782 | MS.gene66437:CDS |
| TCAGCGTTCCACGTTGGTTG+TGG | 0.408075 | 1.4:-4649723 | None:intergenic |
| AAGTAAACGGTTCAATGTTA+AGG | 0.437422 | 1.4:+4649880 | MS.gene66437:CDS |
| ATCTCCAACTTCTTCAAGCT+TGG | 0.439774 | 1.4:-4650668 | None:intergenic |
| GATCGATGATTATGGATTTG+AGG | 0.440058 | 1.4:+4650558 | MS.gene66437:CDS |
| TTGAGGAGGGGTCGGAGTTG+AGG | 0.440541 | 1.4:+4650575 | MS.gene66437:CDS |
| ACGGGAATTAAGCACTACTT+TGG | 0.440901 | 1.4:+4649960 | MS.gene66437:CDS |
| GCCACATGAGTCGCCTTTCA+CGG | 0.446829 | 1.4:+4650010 | MS.gene66437:CDS |
| GTTTAGACTGGAGGGTGTGT+TGG | 0.454377 | 1.4:+4650711 | MS.gene66437:CDS |
| CACACCAAGCTTGAAGAAGT+TGG | 0.454920 | 1.4:+4650664 | MS.gene66437:CDS |
| GTCAGAGATGATGAAGGATA+AGG | 0.460957 | 1.4:+4650600 | MS.gene66437:CDS |
| TTGAGCCTCTGTGGGCATGC+TGG | 0.461766 | 1.4:-4649850 | None:intergenic |
| AGCTTGAAGAAGTTGGAGAT+TGG | 0.472593 | 1.4:+4650671 | MS.gene66437:CDS |
| ATAGGTGTGACAGGAATTGT+AGG | 0.473063 | 1.4:+4649624 | MS.gene66437:CDS |
| GATCTTACCGATTATTTCGA+AGG | 0.473899 | 1.4:-4649985 | None:intergenic |
| TTAAGGTTTCCTGGCTCAAA+AGG | 0.474340 | 1.4:+4650373 | MS.gene66437:CDS |
| TGTGCAGATTACAGAATTCA+AGG | 0.476950 | 1.4:+4649566 | MS.gene66437:CDS |
| TGCACCCCAAATATGTTGTT+CGG | 0.488407 | 1.4:-4650440 | None:intergenic |
| GCTCAAAACTGCGAAGTAAA+CGG | 0.494095 | 1.4:+4649867 | MS.gene66437:CDS |
| GATTCAGAGCATTTGGAATG+AGG | 0.498664 | 1.4:-4649917 | None:intergenic |
| TGGTGGTTAGCTAAAGCATC+TGG | 0.499690 | 1.4:+4649440 | MS.gene66437:CDS |
| ATATGTGTCACATCAGTTAA+GGG | 0.499720 | 1.4:-4649810 | None:intergenic |
| TCCTGGCTCAAAAGGTGCAT+GGG | 0.501130 | 1.4:+4650381 | MS.gene66437:CDS |
| TTGAAGAAGTTGGAGATTGG+TGG | 0.501232 | 1.4:+4650674 | MS.gene66437:CDS |
| TTGAATTCTGTAATCTGCAC+AGG | 0.503753 | 1.4:-4649564 | None:intergenic |
| CGCCGCAATTTGCAAGCATG+AGG | 0.506185 | 1.4:+4650342 | MS.gene66437:CDS |
| TTGTCATGTGTCCCTTCAGA+CGG | 0.511811 | 1.4:+4649941 | MS.gene66437:CDS |
| CTACCTCTCGACGACACACC+AGG | 0.520355 | 1.4:+4649666 | MS.gene66437:CDS |
| ATGAGGTTCATCATGCTGTA+AGG | 0.520609 | 1.4:-4650301 | None:intergenic |
| GCATTGATTATAGGTGTGAC+AGG | 0.521706 | 1.4:+4649615 | MS.gene66437:CDS |
| CGTGGCACATCTTCCGTGAA+AGG | 0.522023 | 1.4:-4650023 | None:intergenic |
| GCCACGCCATAAACCTTCCA+TGG | 0.524352 | 1.4:-4649693 | None:intergenic |
| CAAGTGGAAACATTTGTGGA+AGG | 0.525790 | 1.4:+4650516 | MS.gene66437:CDS |
| TACAGCATGATGAACCTCAT+TGG | 0.533270 | 1.4:+4650304 | MS.gene66437:CDS |
| ACGACACACCAGGTGGTCCA+TGG | 0.534646 | 1.4:+4649676 | MS.gene66437:CDS |
| GAAGGATAAGGGTGGTATGT+GGG | 0.539278 | 1.4:+4650612 | MS.gene66437:CDS |
| GAAGCATTCAACTGTAGTAA+TGG | 0.539621 | 1.4:+4650484 | MS.gene66437:CDS |
| TCAGAGATGATGAAGGATAA+GGG | 0.539728 | 1.4:+4650601 | MS.gene66437:CDS |
| CGTTCCACGTTGGTTGTGGA+CGG | 0.541898 | 1.4:-4649719 | None:intergenic |
| AGTGCTTAATTCCCGTCTGA+AGG | 0.544367 | 1.4:-4649953 | None:intergenic |
| ATTCAGAGCATTTGGAATGA+GGG | 0.546210 | 1.4:-4649916 | None:intergenic |
| TAAACCTTCCATGGACCACC+TGG | 0.546760 | 1.4:-4649684 | None:intergenic |
| GATGATTATGGATTTGAGGA+GGG | 0.546835 | 1.4:+4650562 | MS.gene66437:CDS |
| TGTCATGTGTCCCTTCAGAC+GGG | 0.548530 | 1.4:+4649942 | MS.gene66437:CDS |
| GAGGTTGTCAGAGATGATGA+AGG | 0.549306 | 1.4:+4650594 | MS.gene66437:CDS |
| CGATGATTATGGATTTGAGG+AGG | 0.550813 | 1.4:+4650561 | MS.gene66437:CDS |
| TTCCTGGCTCAAAAGGTGCA+TGG | 0.551639 | 1.4:+4650380 | MS.gene66437:CDS |
| TGAAGGATAAGGGTGGTATG+TGG | 0.552561 | 1.4:+4650611 | MS.gene66437:CDS |
| TTAGCTAAAGCATCTGGCAC+TGG | 0.552955 | 1.4:+4649446 | MS.gene66437:CDS |
| TTATGGATTTGAGGAGGGGT+CGG | 0.555462 | 1.4:+4650567 | MS.gene66437:CDS |
| TCTTGCGTGTGATAGAAATT+GGG | 0.562829 | 1.4:-4650056 | None:intergenic |
| ACCCTCATGCTTGCAAATTG+CGG | 0.571724 | 1.4:-4650344 | None:intergenic |
| ATGAACAAGGCTAAAGAACA+TGG | 0.573832 | 1.4:+4650739 | MS.gene66437:CDS |
| TGTGGAAGGTGTTAGCAGAA+CGG | 0.582640 | 1.4:+4650530 | MS.gene66437:CDS |
| AGAGAATATCTTGCTAGAAG+AGG | 0.582807 | 1.4:+4650076 | MS.gene66437:CDS |
| ACAACATATTTGGGGTGCAG+TGG | 0.589484 | 1.4:+4650444 | MS.gene66437:CDS |
| ATAGCCGAACAACATATTTG+GGG | 0.589709 | 1.4:+4650436 | MS.gene66437:CDS |
| GTGCTTAATTCCCGTCTGAA+GGG | 0.602476 | 1.4:-4649952 | None:intergenic |
| CCACCTGGTGTGTCGTCGAG+AGG | 0.607129 | 1.4:-4649669 | None:intergenic |
| CATTTGGAATGAGGGCTCGA+AGG | 0.609771 | 1.4:-4649908 | None:intergenic |
| GAAAATCCAAAGATAACTTG+TGG | 0.613520 | 1.4:-4650277 | None:intergenic |
| CGAGAGGTAGAATCTCTGCT+AGG | 0.621247 | 1.4:-4649653 | None:intergenic |
| GCCGCAATTTGCAAGCATGA+GGG | 0.625863 | 1.4:+4650343 | MS.gene66437:CDS |
| ATGATTATGGATTTGAGGAG+GGG | 0.626346 | 1.4:+4650563 | MS.gene66437:CDS |
| CACACCAGGTGGTCCATGGA+AGG | 0.631164 | 1.4:+4649680 | MS.gene66437:CDS |
| TGCTTATCAGCGTTCCACGT+TGG | 0.632295 | 1.4:-4649729 | None:intergenic |
| AAACACAAAGCGTGCCAATG+AGG | 0.632651 | 1.4:-4650318 | None:intergenic |
| AAATTGGGTGTGACAAGGCG+TGG | 0.639832 | 1.4:-4650041 | None:intergenic |
| TGTGTTGGATTCTATGAACA+AGG | 0.648254 | 1.4:+4650726 | MS.gene66437:CDS |
| TCCATGGAAGGTTTATGGCG+TGG | 0.649148 | 1.4:+4649692 | MS.gene66437:CDS |
| TCCGTGAAAGGCGACTCATG+TGG | 0.670283 | 1.4:-4650011 | None:intergenic |
| GATAGAAATTGGGTGTGACA+AGG | 0.672850 | 1.4:-4650046 | None:intergenic |
| CTCGCCGTCCACAACCAACG+TGG | 0.681033 | 1.4:+4649715 | MS.gene66437:CDS |
| GAGATGATGAAGGATAAGGG+TGG | 0.696085 | 1.4:+4650604 | MS.gene66437:CDS |
| CCTCTCGACGACACACCAGG+TGG | 0.737440 | 1.4:+4649669 | MS.gene66437:CDS |
| GTGGACCAGCATGCCCACAG+AGG | 0.748657 | 1.4:+4649845 | MS.gene66437:CDS |
| TATGTGGGAAGAGATTGTGA+AGG | 0.782488 | 1.4:+4650627 | MS.gene66437:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTGAAAATAAAAATTAAA+AGG | - | chr1.4:4649518-4649537 | None:intergenic | 10.0% |
| !! | GTTGAAAATAAAAATTAAAA+GGG | - | chr1.4:4649517-4649536 | None:intergenic | 10.0% |
| !! | TTGAAAATAAAAATTAAAAG+GGG | - | chr1.4:4649516-4649535 | None:intergenic | 10.0% |
| !! | TGAAAATAAAAATTAAAAGG+GGG | - | chr1.4:4649515-4649534 | None:intergenic | 15.0% |
| !! | AAAATAAAAATTAAAAGGGG+GGG | - | chr1.4:4649513-4649532 | None:intergenic | 20.0% |
| !! | GAAAATAAAAATTAAAAGGG+GGG | - | chr1.4:4649514-4649533 | None:intergenic | 20.0% |
| !!! | AACTATATTTTTATTCAGGT+GGG | + | chr1.4:4650220-4650239 | MS.gene66437:intron | 20.0% |
| !!! | GATGAACTATATTTTTATTC+AGG | + | chr1.4:4650216-4650235 | MS.gene66437:intron | 20.0% |
| ! | CTATGAATTCATTCATAAGT+TGG | + | chr1.4:4650782-4650801 | MS.gene66437:CDS | 25.0% |
| !! | CTATAATCAATGCTACATTT+TGG | - | chr1.4:4649608-4649627 | None:intergenic | 25.0% |
| !!! | CAAAATGTAGCATTGATTAT+AGG | + | chr1.4:4649606-4649625 | MS.gene66437:CDS | 25.0% |
| !!! | GAACTATATTTTTATTCAGG+TGG | + | chr1.4:4650219-4650238 | MS.gene66437:intron | 25.0% |
| AACATGGTTTCTTAGGATTT+AGG | + | chr1.4:4650755-4650774 | MS.gene66437:CDS | 30.0% | |
| AAGTAAACGGTTCAATGTTA+AGG | + | chr1.4:4649880-4649899 | MS.gene66437:CDS | 30.0% | |
| AATAGCCGAACAACATATTT+GGG | + | chr1.4:4650435-4650454 | MS.gene66437:CDS | 30.0% | |
| AATATGTGTCACATCAGTTA+AGG | - | chr1.4:4649814-4649833 | None:intergenic | 30.0% | |
| AATGCTTCATTCTTTGCATT+AGG | - | chr1.4:4650472-4650491 | None:intergenic | 30.0% | |
| ATATGTGTCACATCAGTTAA+GGG | - | chr1.4:4649813-4649832 | None:intergenic | 30.0% | |
| GAAAATCCAAAGATAACTTG+TGG | - | chr1.4:4650280-4650299 | None:intergenic | 30.0% | |
| GTAAGAAAAAAGCAATGACA+TGG | + | chr1.4:4650242-4650261 | MS.gene66437:CDS | 30.0% | |
| TAATAGCCGAACAACATATT+TGG | + | chr1.4:4650434-4650453 | MS.gene66437:CDS | 30.0% | |
| TTTGGATCCTTCGAAATAAT+CGG | + | chr1.4:4649978-4649997 | MS.gene66437:CDS | 30.0% | |
| ! | AGATTTTATGTTTAGACTGG+AGG | + | chr1.4:4650702-4650721 | MS.gene66437:CDS | 30.0% |
| ! | GATTTTATGTTTAGACTGGA+GGG | + | chr1.4:4650703-4650722 | MS.gene66437:CDS | 30.0% |
| ! | TGCAGATTTTATGTTTAGAC+TGG | + | chr1.4:4650699-4650718 | MS.gene66437:CDS | 30.0% |
| !!! | TTTTCAAGTGGAAACATTTG+TGG | + | chr1.4:4650512-4650531 | MS.gene66437:CDS | 30.0% |
| AATATCTTGCTAGAAGAGGT+TGG | + | chr1.4:4650080-4650099 | MS.gene66437:intron | 35.0% | |
| AATCGACCACAAGTTATCTT+TGG | + | chr1.4:4650271-4650290 | MS.gene66437:CDS | 35.0% | |
| AGAGAATATCTTGCTAGAAG+AGG | + | chr1.4:4650076-4650095 | MS.gene66437:CDS | 35.0% | |
| ATAGCCGAACAACATATTTG+GGG | + | chr1.4:4650436-4650455 | MS.gene66437:CDS | 35.0% | |
| ATGAACAAGGCTAAAGAACA+TGG | + | chr1.4:4650739-4650758 | MS.gene66437:CDS | 35.0% | |
| ATGATTATGGATTTGAGGAG+GGG | + | chr1.4:4650563-4650582 | MS.gene66437:CDS | 35.0% | |
| GATCGATGATTATGGATTTG+AGG | + | chr1.4:4650558-4650577 | MS.gene66437:CDS | 35.0% | |
| GATGATTATGGATTTGAGGA+GGG | + | chr1.4:4650562-4650581 | MS.gene66437:CDS | 35.0% | |
| GCTAAAGAACATGGTTTCTT+AGG | + | chr1.4:4650748-4650767 | MS.gene66437:CDS | 35.0% | |
| TCAGAGATGATGAAGGATAA+GGG | + | chr1.4:4650601-4650620 | MS.gene66437:CDS | 35.0% | |
| TCTTGCGTGTGATAGAAATT+GGG | - | chr1.4:4650059-4650078 | None:intergenic | 35.0% | |
| TGTGCAGATTACAGAATTCA+AGG | + | chr1.4:4649566-4649585 | MS.gene66437:CDS | 35.0% | |
| TGTGTTGGATTCTATGAACA+AGG | + | chr1.4:4650726-4650745 | MS.gene66437:CDS | 35.0% | |
| TTGAATTCTGTAATCTGCAC+AGG | - | chr1.4:4649567-4649586 | None:intergenic | 35.0% | |
| ! | ATTCAGAGCATTTGGAATGA+GGG | - | chr1.4:4649919-4649938 | None:intergenic | 35.0% |
| ! | CACATATTTTCTATGTCTCG+TGG | + | chr1.4:4649826-4649845 | MS.gene66437:CDS | 35.0% |
| ! | GAAGCATTCAACTGTAGTAA+TGG | + | chr1.4:4650484-4650503 | MS.gene66437:CDS | 35.0% |
| ! | GATCTTACCGATTATTTCGA+AGG | - | chr1.4:4649988-4650007 | None:intergenic | 35.0% |
| ! | GCTTCAATTCGACTTCTTTT+TGG | - | chr1.4:4649785-4649804 | None:intergenic | 35.0% |
| ! | TGACAAAGATTCAGAGCATT+TGG | - | chr1.4:4649927-4649946 | None:intergenic | 35.0% |
| !! | CAATTCGACTTCTTTTTGGT+CGG | - | chr1.4:4649781-4649800 | None:intergenic | 35.0% |
| !! | GTAATGGTGATGTTTTCAAG+TGG | + | chr1.4:4650500-4650519 | MS.gene66437:CDS | 35.0% |
| ACGGGAATTAAGCACTACTT+TGG | + | chr1.4:4649960-4649979 | MS.gene66437:CDS | 40.0% | |
| AGCTTGAAGAAGTTGGAGAT+TGG | + | chr1.4:4650671-4650690 | MS.gene66437:CDS | 40.0% | |
| ATAGGTGTGACAGGAATTGT+AGG | + | chr1.4:4649624-4649643 | MS.gene66437:CDS | 40.0% | |
| ATCTCCAACTTCTTCAAGCT+TGG | - | chr1.4:4650671-4650690 | None:intergenic | 40.0% | |
| ATGAGGTTCATCATGCTGTA+AGG | - | chr1.4:4650304-4650323 | None:intergenic | 40.0% | |
| CAAGTGGAAACATTTGTGGA+AGG | + | chr1.4:4650516-4650535 | MS.gene66437:CDS | 40.0% | |
| CGATGATTATGGATTTGAGG+AGG | + | chr1.4:4650561-4650580 | MS.gene66437:CDS | 40.0% | |
| CTCTTGCGTGTGATAGAAAT+TGG | - | chr1.4:4650060-4650079 | None:intergenic | 40.0% | |
| GATAGAAATTGGGTGTGACA+AGG | - | chr1.4:4650049-4650068 | None:intergenic | 40.0% | |
| GCTCAAAACTGCGAAGTAAA+CGG | + | chr1.4:4649867-4649886 | MS.gene66437:CDS | 40.0% | |
| GTCAGAGATGATGAAGGATA+AGG | + | chr1.4:4650600-4650619 | MS.gene66437:CDS | 40.0% | |
| TACAGCATGATGAACCTCAT+TGG | + | chr1.4:4650304-4650323 | MS.gene66437:CDS | 40.0% | |
| TATGTGGGAAGAGATTGTGA+AGG | + | chr1.4:4650627-4650646 | MS.gene66437:CDS | 40.0% | |
| TGCACCCCAAATATGTTGTT+CGG | - | chr1.4:4650443-4650462 | None:intergenic | 40.0% | |
| TTAAGGTTTCCTGGCTCAAA+AGG | + | chr1.4:4650373-4650392 | MS.gene66437:CDS | 40.0% | |
| TTGAAGAAGTTGGAGATTGG+TGG | + | chr1.4:4650674-4650693 | MS.gene66437:CDS | 40.0% | |
| TTTGAGCCAGGAAACCTTAA+AGG | - | chr1.4:4650373-4650392 | None:intergenic | 40.0% | |
| ! | CGGTTTGAGATCGATGATTA+TGG | + | chr1.4:4650550-4650569 | MS.gene66437:CDS | 40.0% |
| ! | GATTCAGAGCATTTGGAATG+AGG | - | chr1.4:4649920-4649939 | None:intergenic | 40.0% |
| !! | GCATTGATTATAGGTGTGAC+AGG | + | chr1.4:4649615-4649634 | MS.gene66437:CDS | 40.0% |
| AAACACAAAGCGTGCCAATG+AGG | - | chr1.4:4650321-4650340 | None:intergenic | 45.0% | |
| ACCCTCATGCTTGCAAATTG+CGG | - | chr1.4:4650347-4650366 | None:intergenic | 45.0% | |
| AGCATGAGGGTCTTCCTTTA+AGG | + | chr1.4:4650356-4650375 | MS.gene66437:CDS | 45.0% | |
| AGTGCTTAATTCCCGTCTGA+AGG | - | chr1.4:4649956-4649975 | None:intergenic | 45.0% | |
| CACACCAAGCTTGAAGAAGT+TGG | + | chr1.4:4650664-4650683 | MS.gene66437:CDS | 45.0% | |
| GAAGGATAAGGGTGGTATGT+GGG | + | chr1.4:4650612-4650631 | MS.gene66437:CDS | 45.0% | |
| GAGATGATGAAGGATAAGGG+TGG | + | chr1.4:4650604-4650623 | MS.gene66437:CDS | 45.0% | |
| GAGGTTGTCAGAGATGATGA+AGG | + | chr1.4:4650594-4650613 | MS.gene66437:CDS | 45.0% | |
| GTGCTTAATTCCCGTCTGAA+GGG | - | chr1.4:4649955-4649974 | None:intergenic | 45.0% | |
| TGAAGGATAAGGGTGGTATG+TGG | + | chr1.4:4650611-4650630 | MS.gene66437:CDS | 45.0% | |
| TTATGGATTTGAGGAGGGGT+CGG | + | chr1.4:4650567-4650586 | MS.gene66437:CDS | 45.0% | |
| TTGTCATGTGTCCCTTCAGA+CGG | + | chr1.4:4649941-4649960 | MS.gene66437:CDS | 45.0% | |
| ! | ACAACATATTTGGGGTGCAG+TGG | + | chr1.4:4650444-4650463 | MS.gene66437:CDS | 45.0% |
| ! | TGGTGGTTAGCTAAAGCATC+TGG | + | chr1.4:4649440-4649459 | MS.gene66437:CDS | 45.0% |
| ! | TTAGCTAAAGCATCTGGCAC+TGG | + | chr1.4:4649446-4649465 | MS.gene66437:CDS | 45.0% |
| !! | GCTACATTTTGGAAGCTTCG+CGG | - | chr1.4:4649597-4649616 | None:intergenic | 45.0% |
| !! | GGTCTTCCTTTAAGGTTTCC+TGG | + | chr1.4:4650364-4650383 | MS.gene66437:CDS | 45.0% |
| !! | TGTGGAAGGTGTTAGCAGAA+CGG | + | chr1.4:4650530-4650549 | MS.gene66437:CDS | 45.0% |
| AAATTGGGTGTGACAAGGCG+TGG | - | chr1.4:4650044-4650063 | None:intergenic | 50.0% | |
| CGAGAGGTAGAATCTCTGCT+AGG | - | chr1.4:4649656-4649675 | None:intergenic | 50.0% | |
| GCCGCAATTTGCAAGCATGA+GGG | + | chr1.4:4650343-4650362 | MS.gene66437:CDS | 50.0% | |
| GGTGGTCCATGGAAGGTTTA+TGG | + | chr1.4:4649687-4649706 | MS.gene66437:CDS | 50.0% | |
| TAAACCTTCCATGGACCACC+TGG | - | chr1.4:4649687-4649706 | None:intergenic | 50.0% | |
| TCCATGGAAGGTTTATGGCG+TGG | + | chr1.4:4649692-4649711 | MS.gene66437:CDS | 50.0% | |
| TGCTTATCAGCGTTCCACGT+TGG | - | chr1.4:4649732-4649751 | None:intergenic | 50.0% | |
| TGTCATGTGTCCCTTCAGAC+GGG | + | chr1.4:4649942-4649961 | MS.gene66437:CDS | 50.0% | |
| ! | CATTTGGAATGAGGGCTCGA+AGG | - | chr1.4:4649911-4649930 | None:intergenic | 50.0% |
| ! | GTTTAGACTGGAGGGTGTGT+TGG | + | chr1.4:4650711-4650730 | MS.gene66437:CDS | 50.0% |
| ! | TCCTGGCTCAAAAGGTGCAT+GGG | + | chr1.4:4650381-4650400 | MS.gene66437:CDS | 50.0% |
| ! | TTCCTGGCTCAAAAGGTGCA+TGG | + | chr1.4:4650380-4650399 | MS.gene66437:CDS | 50.0% |
| !!! | TCGCAGTTTTGAGCCTCTGT+GGG | - | chr1.4:4649861-4649880 | None:intergenic | 50.0% |
| !!! | TTCGCAGTTTTGAGCCTCTG+TGG | - | chr1.4:4649862-4649881 | None:intergenic | 50.0% |
| CGCCGCAATTTGCAAGCATG+AGG | + | chr1.4:4650342-4650361 | MS.gene66437:CDS | 55.0% | |
| CGTGGCACATCTTCCGTGAA+AGG | - | chr1.4:4650026-4650045 | None:intergenic | 55.0% | |
| GCCACATGAGTCGCCTTTCA+CGG | + | chr1.4:4650010-4650029 | MS.gene66437:CDS | 55.0% | |
| GCCACGCCATAAACCTTCCA+TGG | - | chr1.4:4649696-4649715 | None:intergenic | 55.0% | |
| TCCGTGAAAGGCGACTCATG+TGG | - | chr1.4:4650014-4650033 | None:intergenic | 55.0% | |
| ! | CGTTCCACGTTGGTTGTGGA+CGG | - | chr1.4:4649722-4649741 | None:intergenic | 55.0% |
| ! | TCAGCGTTCCACGTTGGTTG+TGG | - | chr1.4:4649726-4649745 | None:intergenic | 55.0% |
| ! | TCCCATGCACCTTTTGAGCC+AGG | - | chr1.4:4650385-4650404 | None:intergenic | 55.0% |
| ACGACACACCAGGTGGTCCA+TGG | + | chr1.4:4649676-4649695 | MS.gene66437:CDS | 60.0% | |
| CACACCAGGTGGTCCATGGA+AGG | + | chr1.4:4649680-4649699 | MS.gene66437:CDS | 60.0% | |
| CTACCTCTCGACGACACACC+AGG | + | chr1.4:4649666-4649685 | MS.gene66437:CDS | 60.0% | |
| TTGAGCCTCTGTGGGCATGC+TGG | - | chr1.4:4649853-4649872 | None:intergenic | 60.0% | |
| TTGAGGAGGGGTCGGAGTTG+AGG | + | chr1.4:4650575-4650594 | MS.gene66437:CDS | 60.0% | |
| CCACCTGGTGTGTCGTCGAG+AGG | - | chr1.4:4649672-4649691 | None:intergenic | 65.0% | |
| CCTCTCGACGACACACCAGG+TGG | + | chr1.4:4649669-4649688 | MS.gene66437:CDS | 65.0% | |
| CTCGCCGTCCACAACCAACG+TGG | + | chr1.4:4649715-4649734 | MS.gene66437:CDS | 65.0% | |
| GTGGACCAGCATGCCCACAG+AGG | + | chr1.4:4649845-4649864 | MS.gene66437:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 4649434 | 4650840 | 4649434 | ID=MS.gene66437 |
| chr1.4 | mRNA | 4649434 | 4650840 | 4649434 | ID=MS.gene66437.t1;Parent=MS.gene66437 |
| chr1.4 | exon | 4649434 | 4649476 | 4649434 | ID=MS.gene66437.t1.exon1;Parent=MS.gene66437.t1 |
| chr1.4 | CDS | 4649434 | 4649476 | 4649434 | ID=cds.MS.gene66437.t1;Parent=MS.gene66437.t1 |
| chr1.4 | exon | 4649544 | 4650097 | 4649544 | ID=MS.gene66437.t1.exon2;Parent=MS.gene66437.t1 |
| chr1.4 | CDS | 4649544 | 4650097 | 4649544 | ID=cds.MS.gene66437.t1;Parent=MS.gene66437.t1 |
| chr1.4 | exon | 4650238 | 4650840 | 4650238 | ID=MS.gene66437.t1.exon3;Parent=MS.gene66437.t1 |
| chr1.4 | CDS | 4650238 | 4650840 | 4650238 | ID=cds.MS.gene66437.t1;Parent=MS.gene66437.t1 |
| Gene Sequence |
| Protein sequence |
