AlfalfaGEDB | MS.gene68956

M. sativa cultivar XinJiangDaYe / MS.gene68956

KEGG: K11147
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68956.t1	XP_003610496.1	99.6	252	1	0	1	252	1	252	2.30E-130	474.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68956.t1	H9BFQ2	78.4	250	54	0	3	252	9	258	2.4e-104	379.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68956.t1	G7JKG6	99.6	252	1	0	1	252	1	252	1.6e-130	474.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68956	MS.gene021442	PPI
MS.gene62193	MS.gene68956	PPI
MS.gene68956	MS.gene007678	PPI
MS.gene68956	MS.gene028938	PPI
MS.gene68956	MS.gene003992	PPI
MS.gene68956	MS.gene30551	PPI
MS.gene68956	MS.gene63306	PPI
MS.gene68956	MS.gene055846	PPI
MS.gene68956	MS.gene62195	PPI
MS.gene050432	MS.gene68956	PPI
MS.gene68956	MS.gene31648	PPI
MS.gene68956	MS.gene63300	PPI
MS.gene68956	MS.gene85724	PPI
MS.gene68956	MS.gene018066	PPI
MS.gene68956	MS.gene80360	PPI
MS.gene68956	MS.gene008160	PPI
MS.gene62194	MS.gene68956	PPI
MS.gene63307	MS.gene68956	PPI
MS.gene68956	MS.gene019786	PPI
MS.gene68956	MS.gene050131	PPI
MS.gene68956	MS.gene52743	PPI
MS.gene008162	MS.gene68956	PPI
MS.gene68956	MS.gene072664	PPI
MS.gene68956	MS.gene07097	PPI
MS.gene68956	MS.gene021164	PPI
MS.gene54548	MS.gene68956	PPI
MS.gene68956	MS.gene07096	PPI
MS.gene08694	MS.gene68956	PPI
MS.gene68956	MS.gene63483	PPI
MS.gene68956	MS.gene50524	PPI
MS.gene68956	MS.gene50522	PPI
MS.gene63484	MS.gene68956	PPI
MS.gene68956	MS.gene63482	PPI
MS.gene68956	MS.gene52742	PPI
MS.gene68956	MS.gene04700	PPI
MS.gene68956	MS.gene31507	PPI
MS.gene68956	MS.gene28847	PPI
MS.gene68956	MS.gene059170	PPI
MS.gene68956	MS.gene08695	PPI
MS.gene68956	MS.gene008161	PPI
MS.gene050431	MS.gene68956	PPI
MS.gene63403	MS.gene68956	PPI
MS.gene41122	MS.gene68956	PPI
MS.gene68956	MS.gene62187	PPI
MS.gene073684	MS.gene68956	PPI
MS.gene63308	MS.gene68956	PPI
MS.gene033685	MS.gene68956	PPI
MS.gene68956	MS.gene03319	PPI
MS.gene68956	MS.gene043387	PPI
MS.gene68956	MS.gene008153	PPI
MS.gene28848	MS.gene68956	PPI
MS.gene68956	MS.gene08756	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68956.t1	MTR_4g132850	99.603	252	1	0	1	252	1	252	0.0	509
MS.gene68956.t1	MTR_3g068070	33.333	246	159	4	7	248	15	259	2.17e-36	130
MS.gene68956.t1	MTR_3g068065	32.927	246	160	4	7	248	17	261	1.44e-35	128
MS.gene68956.t1	MTR_3g068175	35.366	246	154	4	7	248	17	261	1.27e-34	125
MS.gene68956.t1	MTR_7g063270	33.984	256	157	7	5	250	12	265	3.17e-34	124
MS.gene68956.t1	MTR_3g068085	31.707	246	163	4	7	248	15	259	9.35e-34	123
MS.gene68956.t1	MTR_4g064887	32.114	246	162	4	7	248	15	259	4.05e-33	121
MS.gene68956.t1	MTR_4g064883	32.932	249	156	5	7	248	14	258	1.49e-32	120
MS.gene68956.t1	MTR_3g068175	34.800	250	154	5	7	248	17	265	2.05e-32	120
MS.gene68956.t1	MTR_7g063260	33.984	256	158	7	5	250	12	266	1.38e-31	117
MS.gene68956.t1	MTR_3g068155	31.967	244	161	4	9	248	21	263	1.52e-30	115
MS.gene68956.t1	MTR_7g063320	31.200	250	163	5	7	248	15	263	4.83e-29	110
MS.gene68956.t1	MTR_7g063330	32.271	251	162	5	7	250	15	264	2.65e-28	108
MS.gene68956.t1	MTR_6g023610	32.271	251	157	6	6	246	12	259	1.86e-27	106
MS.gene68956.t1	MTR_1g082760	29.249	253	165	6	6	247	39	288	9.38e-27	105
MS.gene68956.t1	MTR_7g107670	28.846	260	166	7	5	250	31	285	1.10e-26	105
MS.gene68956.t1	MTR_6g023990	31.765	255	157	6	6	248	13	262	1.64e-26	103
MS.gene68956.t1	MTR_7g071680	31.502	273	158	9	4	250	26	295	2.80e-26	104
MS.gene68956.t1	MTR_6g023590	32.031	256	155	7	6	248	12	261	5.79e-26	102
MS.gene68956.t1	MTR_7g113650	27.559	254	170	6	5	247	38	288	9.01e-26	102
MS.gene68956.t1	MTR_6g023910	31.076	251	160	6	6	246	12	259	1.11e-25	102
MS.gene68956.t1	MTR_7g063360	32.400	250	160	5	7	248	16	264	1.18e-25	102
MS.gene68956.t1	MTR_7g063390	31.200	250	163	5	7	248	16	264	1.88e-25	101
MS.gene68956.t1	MTR_1196s0010	32.283	254	150	7	5	248	272	513	5.75e-25	103
MS.gene68956.t1	MTR_3g085740	31.128	257	159	8	2	248	70	318	7.33e-25	100
MS.gene68956.t1	MTR_6g023600	33.463	257	150	8	6	248	12	261	1.24e-24	99.0
MS.gene68956.t1	MTR_4g078630	30.916	262	161	7	4	250	16	272	1.73e-24	99.0
MS.gene68956.t1	MTR_7g105460	31.250	256	161	7	3	248	9	259	3.35e-24	98.2
MS.gene68956.t1	MTR_7g083870	30.385	260	163	9	5	250	33	288	5.83e-24	98.2
MS.gene68956.t1	MTR_3g020670	34.340	265	144	10	5	248	14	269	6.45e-24	97.4
MS.gene68956.t1	MTR_7g063270	30.315	254	120	7	5	250	12	216	8.57e-24	95.9
MS.gene68956.t1	MTR_6g023950	31.225	253	161	7	6	248	12	261	3.35e-22	92.4
MS.gene68956.t1	MTR_7g094510	30.120	249	165	7	7	247	6	253	4.98e-22	92.0
MS.gene68956.t1	MTR_4g131740	29.297	256	164	8	6	248	39	290	1.22e-21	91.7
MS.gene68956.t1	MTR_4g064887	31.606	193	127	4	7	195	15	206	1.47e-21	90.1
MS.gene68956.t1	MTR_4g052350	28.458	253	165	7	5	248	12	257	5.68e-21	89.4
MS.gene68956.t1	MTR_4g052300	27.490	251	168	6	6	248	7	251	2.70e-20	87.4
MS.gene68956.t1	MTR_7g094520	29.762	252	164	8	7	248	11	259	3.25e-20	87.0
MS.gene68956.t1	MTR_8g442750	30.469	256	166	4	4	248	11	265	3.65e-20	87.8
MS.gene68956.t1	MTR_4g052340	28.185	259	158	10	3	246	10	255	5.38e-20	86.7
MS.gene68956.t1	MTR_7g110130	29.921	254	166	6	5	249	4	254	7.95e-20	86.3
MS.gene68956.t1	MTR_6g023600	32.685	257	146	9	6	248	12	255	8.71e-20	85.9
MS.gene68956.t1	MTR_2g090165	28.175	252	170	5	5	248	4	252	1.70e-19	85.1
MS.gene68956.t1	MTR_4g064883	31.250	192	121	5	7	191	14	201	2.17e-19	84.0
MS.gene68956.t1	MTR_7g105590	30.688	189	125	5	3	187	4	190	2.58e-19	83.2
MS.gene68956.t1	MTR_4g052320	27.381	252	168	7	6	248	7	252	5.33e-18	81.3
MS.gene68956.t1	MTR_7g105580	27.843	255	151	7	3	247	4	235	6.79e-18	80.9
MS.gene68956.t1	MTR_6g035395	30.196	255	152	9	3	248	1	238	7.67e-17	77.8
MS.gene68956.t1	MTR_7g110140	30.196	255	159	7	6	248	5	252	9.30e-17	77.8
MS.gene68956.t1	MTR_8g096620	26.667	225	147	4	3	222	41	252	4.28e-16	77.0
MS.gene68956.t1	MTR_7g105570	32.418	182	110	6	77	248	38	216	7.61e-16	74.7
MS.gene68956.t1	MTR_4g058840	27.757	263	168	9	1	247	5	261	8.42e-16	75.1
MS.gene68956.t1	MTR_4g058820	27.757	263	168	9	1	247	5	261	8.42e-16	75.1
MS.gene68956.t1	MTR_4g058810	25.097	259	180	7	1	247	5	261	1.51e-14	71.6
MS.gene68956.t1	MTR_7g110160	27.092	251	158	6	4	248	3	234	2.12e-14	70.9
MS.gene68956.t1	MTR_1g068955	27.413	259	164	7	4	246	11	261	4.15e-14	70.5
MS.gene68956.t1	MTR_8g044240	27.979	193	126	6	13	193	75	266	1.21e-13	69.7
MS.gene68956.t1	MTR_8g096580	26.042	192	131	5	10	194	48	235	1.07e-12	67.0
MS.gene68956.t1	MTR_7g080410	27.513	189	130	5	10	191	41	229	1.43e-12	66.2
MS.gene68956.t1	MTR_7g080410	27.513	189	130	5	10	191	41	229	2.26e-12	65.9
MS.gene68956.t1	MTR_4g084800	30.075	133	90	2	2	133	8	138	2.99e-12	63.2
MS.gene68956.t1	MTR_3g113870	26.776	183	127	5	15	193	29	208	5.86e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68956.t1	AT4G05530	73.705	251	66	0	2	252	4	254	5.24e-136	383
MS.gene68956.t1	AT5G06060	34.252	254	160	5	1	248	1	253	1.74e-36	130
MS.gene68956.t1	AT2G29150	34.538	249	149	8	7	247	16	258	1.78e-33	122
MS.gene68956.t1	AT2G29260	33.333	246	159	4	7	248	68	312	7.73e-32	119
MS.gene68956.t1	AT2G29360	33.197	244	158	5	9	248	18	260	7.78e-31	115
MS.gene68956.t1	AT2G29340	32.540	252	159	6	5	248	3	251	4.64e-30	113
MS.gene68956.t1	AT2G29340	32.540	252	159	6	5	248	3	251	8.96e-30	113
MS.gene68956.t1	AT2G29370	30.435	253	164	6	4	248	12	260	6.76e-28	107
MS.gene68956.t1	AT2G29290	31.621	253	160	7	5	248	3	251	1.74e-27	106
MS.gene68956.t1	AT2G29330	32.016	253	159	7	5	248	3	251	1.76e-27	106
MS.gene68956.t1	AT3G26760	30.502	259	162	7	5	248	34	289	3.11e-27	106
MS.gene68956.t1	AT2G29350	32.800	250	148	8	9	247	17	257	1.51e-25	101
MS.gene68956.t1	AT3G05260	29.921	254	163	7	6	248	36	285	2.08e-25	101
MS.gene68956.t1	AT1G07440	30.488	246	165	5	7	248	12	255	6.51e-25	100
MS.gene68956.t1	AT2G30670	31.250	256	157	8	5	248	3	251	9.83e-25	99.4
MS.gene68956.t1	AT2G29300	29.845	258	163	8	3	248	1	252	1.37e-24	99.0
MS.gene68956.t1	AT2G29300	29.845	258	163	8	3	248	1	252	2.26e-24	99.0
MS.gene68956.t1	AT2G47130	33.068	251	154	6	6	247	5	250	3.11e-24	97.8
MS.gene68956.t1	AT1G54870	27.668	253	169	5	6	247	81	330	9.32e-24	98.2
MS.gene68956.t1	AT1G54870	27.668	253	169	5	6	247	83	332	9.38e-24	98.2
MS.gene68956.t1	AT1G07450	30.078	256	166	7	1	248	1	251	1.59e-23	96.3
MS.gene68956.t1	AT2G47120	30.682	264	154	10	6	251	5	257	3.14e-23	95.1
MS.gene68956.t1	AT2G47120	30.682	264	154	10	6	251	6	258	3.44e-23	95.1
MS.gene68956.t1	AT3G04000	29.057	265	161	7	4	247	11	269	4.35e-23	95.1
MS.gene68956.t1	AT2G29320	30.159	252	160	7	7	248	13	258	4.67e-23	95.1
MS.gene68956.t1	AT3G26770	29.615	260	161	9	5	248	39	292	5.24e-23	95.5
MS.gene68956.t1	AT4G13180	31.600	250	158	7	9	248	15	261	7.18e-23	94.4
MS.gene68956.t1	AT5G18210	32.400	250	155	8	9	246	10	257	7.75e-23	94.4
MS.gene68956.t1	AT2G29290	30.303	231	150	6	25	248	8	234	2.47e-22	92.4
MS.gene68956.t1	AT3G51680	30.769	273	158	9	4	250	29	296	5.47e-22	92.8
MS.gene68956.t1	AT3G29260	31.102	254	157	7	5	247	4	250	1.79e-21	90.5
MS.gene68956.t1	AT3G47350	29.048	210	133	5	9	204	46	253	1.93e-21	91.7
MS.gene68956.t1	AT3G47350	29.048	210	133	5	9	204	46	253	2.46e-21	91.3
MS.gene68956.t1	AT2G29310	29.249	253	166	6	5	248	3	251	3.47e-21	89.7
MS.gene68956.t1	AT3G29250	32.946	258	150	11	6	249	5	253	3.99e-21	89.7
MS.gene68956.t1	AT2G47140	30.620	258	154	7	5	247	4	251	5.05e-21	89.4
MS.gene68956.t1	AT1G24360	28.794	257	165	8	2	248	69	317	5.51e-21	90.5
MS.gene68956.t1	AT3G42960	31.298	262	159	8	5	250	6	262	7.05e-21	89.4
MS.gene68956.t1	AT3G29250	32.283	254	155	9	6	248	41	288	1.01e-20	89.4
MS.gene68956.t1	AT3G03980	28.958	259	168	7	4	247	11	268	2.26e-20	87.8
MS.gene68956.t1	AT4G03140	26.562	256	173	7	5	248	76	328	4.04e-20	88.2
MS.gene68956.t1	AT1G63380	30.916	262	150	11	7	247	17	268	6.79e-20	86.7
MS.gene68956.t1	AT1G63380	30.916	262	150	11	7	247	15	266	7.16e-20	86.7
MS.gene68956.t1	AT1G63380	30.916	262	150	11	7	247	19	270	7.29e-20	86.7
MS.gene68956.t1	AT3G29250	32.271	251	153	9	9	248	46	290	8.17e-20	86.7
MS.gene68956.t1	AT1G63380	30.916	262	150	11	7	247	22	273	8.86e-20	86.7
MS.gene68956.t1	AT2G29350	32.301	226	133	8	33	247	3	219	9.54e-20	85.5
MS.gene68956.t1	AT3G12800	29.885	261	162	6	4	248	7	262	1.01e-19	86.7
MS.gene68956.t1	AT1G52340	28.986	276	147	10	5	248	16	274	2.87e-19	85.1
MS.gene68956.t1	AT1G62610	30.682	264	148	11	7	247	19	270	1.23e-18	83.2
MS.gene68956.t1	AT1G62610	30.682	264	148	11	7	247	13	264	1.24e-18	83.2
MS.gene68956.t1	AT1G62610	30.682	264	148	11	7	247	14	265	1.27e-18	83.2
MS.gene68956.t1	AT5G18210	32.203	236	146	8	9	232	10	243	1.35e-18	83.2
MS.gene68956.t1	AT1G62610	30.682	264	148	11	7	247	15	266	1.40e-18	83.2
MS.gene68956.t1	AT3G47360	29.947	187	125	4	10	192	48	232	2.18e-18	83.2
MS.gene68956.t1	AT2G29320	29.237	236	151	7	23	248	11	240	3.49e-18	81.6
MS.gene68956.t1	AT3G55290	28.906	256	162	9	7	247	18	268	6.14e-18	81.3
MS.gene68956.t1	AT3G55290	28.906	256	162	9	7	247	17	267	6.97e-18	81.3
MS.gene68956.t1	AT2G29340	30.769	195	128	5	3	191	1	194	9.51e-18	79.3
MS.gene68956.t1	AT2G29340	30.769	195	128	5	3	191	1	194	9.51e-18	79.3
MS.gene68956.t1	AT3G46170	29.119	261	157	10	6	247	25	276	4.43e-17	79.0
MS.gene68956.t1	AT2G17845	27.027	259	166	9	7	247	47	300	5.07e-17	79.3
MS.gene68956.t1	AT3G55310	28.195	266	163	10	1	247	11	267	3.57e-16	76.6
MS.gene68956.t1	AT5G50600	27.128	188	125	5	9	189	47	229	5.98e-16	76.6
MS.gene68956.t1	AT5G50700	27.128	188	125	5	9	189	47	229	5.98e-16	76.6
MS.gene68956.t1	AT2G29350	31.579	190	119	6	9	191	17	202	6.48e-16	74.7
MS.gene68956.t1	AT2G29310	27.897	233	157	5	23	248	21	249	9.33e-16	75.1
MS.gene68956.t1	AT5G50690	27.513	189	131	4	5	189	43	229	5.40e-15	72.8
MS.gene68956.t1	AT5G50590	27.513	189	131	4	5	189	43	229	5.40e-15	72.8
MS.gene68956.t1	AT5G50690	27.513	189	131	4	5	189	43	229	1.45e-14	72.0
MS.gene68956.t1	AT5G50590	27.513	189	131	4	5	189	43	229	1.45e-14	72.0
MS.gene68956.t1	AT3G03330	28.646	192	129	5	8	191	42	233	2.18e-13	68.9
MS.gene68956.t1	AT5G04900	28.272	191	127	4	13	193	83	273	2.52e-13	68.9
MS.gene68956.t1	AT5G50770	25.980	204	137	5	9	203	47	245	2.52e-11	63.2
MS.gene68956.t1	AT4G10020	25.143	175	125	3	5	175	45	217	2.95e-11	63.2

Find 61 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTCCTTTGAATCTCTT+TGG	0.229084	8.2:+1445271	None:intergenic
TTTGGCTTGTTACAGAAGTA+TGG	0.300714	8.2:-1444492	MS.gene68956:intron
GAAGAACCCTTCGGCAAGTA+TGG	0.332535	8.2:+1444152	None:intergenic
AAGGCCAAGAAGGGCTGTTT+TGG	0.335058	8.2:+1444064	None:intergenic
ATTCGGTCCTTGACAAGTTA+TGG	0.346257	8.2:-1444401	MS.gene68956:CDS
GTAACCAAAACAGCCCTTCT+TGG	0.370781	8.2:-1444068	MS.gene68956:CDS
ACTTGTCAAGGACCGAATCT+TGG	0.376699	8.2:+1444406	None:intergenic
CTTGAAGCAAAGACATTACT+TGG	0.379309	8.2:-1443544	MS.gene68956:CDS
TTATCACCCACCAGCTAGTA+TGG	0.380678	8.2:-1444102	MS.gene68956:CDS
ACTCACCATAGCAGAATTAC+TGG	0.391347	8.2:+1443850	None:intergenic
TGCAAATACATCAATTCCTT+TGG	0.391428	8.2:+1444940	None:intergenic
TTCGGTCCTTGACAAGTTAT+GGG	0.394972	8.2:-1444400	MS.gene68956:CDS
ACTGCTTCAACACAGGGAAT+TGG	0.396053	8.2:-1445238	MS.gene68956:CDS
GGAGAAACTATAGTAGTTTC+CGG	0.398231	8.2:-1443448	MS.gene68956:CDS
CATTGCTGAGAGACTTGGTT+TGG	0.401132	8.2:-1445209	MS.gene68956:CDS
GATGATGCGTCATATATAAC+AGG	0.401523	8.2:-1443469	MS.gene68956:CDS
TAGGGGCCATCTCAGCAGCC+AGG	0.410588	8.2:+1443933	None:intergenic
CGAATCTTGGGTTTGCAGAA+TGG	0.413839	8.2:+1444419	None:intergenic
CTTGTCAAGGACCGAATCTT+GGG	0.415114	8.2:+1444407	None:intergenic
GTAGGCCCTGGCTGCTGAGA+TGG	0.440474	8.2:-1443939	MS.gene68956:intron
AGAAGGATTTGCAGCAGCAT+TGG	0.451910	8.2:+1444449	None:intergenic
TGTTATCTCTTCTCGCAGAC+AGG	0.457846	8.2:-1445173	MS.gene68956:intron
GATCGTGACTGCTTCAACAC+AGG	0.459705	8.2:-1445245	MS.gene68956:CDS
CCAGCTAGTATGGCTATGTA+TGG	0.467840	8.2:-1444092	MS.gene68956:CDS
TTTACCATTGCTGAGAGACT+TGG	0.472945	8.2:-1445214	MS.gene68956:CDS
TACCTTCAGAAGAAGTATAG+TGG	0.476458	8.2:+1444362	None:intergenic
CGTGTAAACTGTGTTGCTCC+TGG	0.479482	8.2:-1443905	MS.gene68956:CDS
CATTCTGCAAACCCAAGATT+CGG	0.481732	8.2:-1444418	MS.gene68956:CDS
TGTAGCAGCACCCATGTCTT+CGG	0.486116	8.2:+1443507	None:intergenic
AGGGGCCATCTCAGCAGCCA+GGG	0.499508	8.2:+1443934	None:intergenic
GTTATCATTTCATCGATCGC+TGG	0.504376	8.2:-1444125	MS.gene68956:CDS
CGACTTTGGTAAGGCCAAGA+AGG	0.505337	8.2:+1444054	None:intergenic
ATGAATGAAGCAAAATTAGT+TGG	0.506154	8.2:+1443875	None:intergenic
CTCAAATGCTCTACAAAGGA+AGG	0.512057	8.2:-1444903	MS.gene68956:CDS
GAAATTCCAAAGAGATTCAA+AGG	0.522716	8.2:-1445277	MS.gene68956:CDS
ACTTGGTACAACCGAAGACA+TGG	0.533738	8.2:-1443518	MS.gene68956:CDS
AAGACATTACTTGGAAGACT+TGG	0.543455	8.2:-1443535	MS.gene68956:CDS
TTTGATTGACAAAACTGTCC+AGG	0.545328	8.2:-1444879	MS.gene68956:intron
TTTGTAGAGCATTTGAGACA+TGG	0.550170	8.2:+1444909	None:intergenic
TCCAAAGAGATTCAAAGGAA+AGG	0.550796	8.2:-1445272	MS.gene68956:CDS
GGCAAGTACCGACTTTGGTA+AGG	0.550957	8.2:+1444045	None:intergenic
CATCACCAGTAATTCTGCTA+TGG	0.551246	8.2:-1443855	MS.gene68956:intron
GACTTTGGTAAGGCCAAGAA+GGG	0.555272	8.2:+1444055	None:intergenic
GCTGAGAGACTTGGTTTGGA+AGG	0.565863	8.2:-1445205	MS.gene68956:CDS
TTATCTCCCATAACTTGTCA+AGG	0.576972	8.2:+1444394	None:intergenic
TGCAGAATGGAATCAACAGA+AGG	0.579952	8.2:+1444432	None:intergenic
CCATACATAGCCATACTAGC+TGG	0.584897	8.2:+1444092	None:intergenic
TGCAGCTCCATACTTGCCGA+AGG	0.587100	8.2:-1444159	MS.gene68956:CDS
CAGTCTCGATGGCATTCCAC+CGG	0.593625	8.2:+1443429	None:intergenic
ATCGTGACTGCTTCAACACA+GGG	0.594859	8.2:-1445244	MS.gene68956:CDS
AGCCACTATACTTCTTCTGA+AGG	0.599186	8.2:-1444364	MS.gene68956:intron
TACATAGCCATACTAGCTGG+TGG	0.601638	8.2:+1444095	None:intergenic
AAATTAGTTGGTACAAAACC+AGG	0.611900	8.2:+1443887	None:intergenic
ATAACAACAGAAGAACCCTT+CGG	0.615477	8.2:+1444143	None:intergenic
CAAACCAAGTCTCTCAGCAA+TGG	0.620172	8.2:+1445210	None:intergenic
AATGGAAGCTACAGTCTCGA+TGG	0.627135	8.2:+1443418	None:intergenic
ATGTCTCAAATGCTCTACAA+AGG	0.639990	8.2:-1444907	MS.gene68956:CDS
ACATAGCCATACTAGCTGGT+GGG	0.645983	8.2:+1444096	None:intergenic
GCAGCTCCATACTTGCCGAA+GGG	0.672297	8.2:-1444158	MS.gene68956:CDS
CTTGGTACAACCGAAGACAT+GGG	0.677968	8.2:-1443517	MS.gene68956:CDS
GAAACTATAGTAGTTTCCGG+TGG	0.686196	8.2:-1443445	MS.gene68956:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATATATATATATATAT+TGG	+	chr8.2:1444435-1444416	None:intergenic	0.0%
!!	ACCAAATTAAAAAAATAATA+TGG	+	chr8.2:1443699-1443718	None:intergenic	10.0%
!!!	TCCATATTATTTTTTTAATT+TGG	-	chr8.2:1443695-1443714	MS.gene68956:intron	10.0%
!!	ATTATGTAAATATAATGTGT+AGG	-	chr8.2:1444749-1444768	MS.gene68956:intron	15.0%
!!!	AATATGTGTTTGAATTTTTT+TGG	-	chr8.2:1444196-1444215	MS.gene68956:intron	15.0%
!!	AAAAACAGTAACAATTCATA+TGG	+	chr8.2:1444709-1444728	None:intergenic	20.0%
!!	GAATGAATCAAATAAGAAAA+AGG	+	chr8.2:1443566-1443585	None:intergenic	20.0%
!!	GTAAATTATTGTATGAGAAT+TGG	-	chr8.2:1443610-1443629	MS.gene68956:intron	20.0%
!!	TGTACAAGTTTAAATAGAAT+TGG	-	chr8.2:1444068-1444087	MS.gene68956:CDS	20.0%
!!	TTGTACAAAATATGAATGAT+GGG	+	chr8.2:1444055-1444074	None:intergenic	20.0%
!!!	ATGTTTTGTCATTATGATTT+TGG	-	chr8.2:1444099-1444118	MS.gene68956:CDS	20.0%
!	ACAAGTTTAAATAGAATTGG+CGG	-	chr8.2:1444071-1444090	MS.gene68956:CDS	25.0%
!	ATATATCATTTGTGTCTGAA+TGG	-	chr8.2:1445107-1445126	MS.gene68956:intron	25.0%
!	ATGAATGAAGCAAAATTAGT+TGG	+	chr8.2:1444834-1444853	None:intergenic	25.0%
!	CATATAGGATAGTTGTAAAA+TGG	+	chr8.2:1443955-1443974	None:intergenic	25.0%
!	CTTGTACAAAATATGAATGA+TGG	+	chr8.2:1444056-1444075	None:intergenic	25.0%
!	TAAGACATCAAATCTCATAT+AGG	+	chr8.2:1443970-1443989	None:intergenic	25.0%
!!	GAGTATCTTTTTAACCAAAT+AGG	-	chr8.2:1444875-1444894	MS.gene68956:intron	25.0%
	AGGTAATTAAGCTGTGAAAA+TGG	-	chr8.2:1444362-1444381	MS.gene68956:intron	30.0%
	ATATTGAAGGAACAATTGGA+AGG	+	chr8.2:1444685-1444704	None:intergenic	30.0%
	CAATTCATATGGCATATTGA+AGG	+	chr8.2:1444698-1444717	None:intergenic	30.0%
	GAAATTCCAAAGAGATTCAA+AGG	-	chr8.2:1443429-1443448	MS.gene68956:CDS	30.0%
	TGCAAATACATCAATTCCTT+TGG	+	chr8.2:1443769-1443788	None:intergenic	30.0%
	TGTCTTTCTCACTATTTGAA+TGG	-	chr8.2:1444167-1444186	MS.gene68956:intron	30.0%
!	AAATTAGTTGGTACAAAACC+AGG	+	chr8.2:1444822-1444841	None:intergenic	30.0%
!	AGTGTGTTGATGTAGTAAAA+TGG	-	chr8.2:1444502-1444521	MS.gene68956:intron	30.0%
!	CACAAGTCTTGTATTGAAAT+TGG	+	chr8.2:1443888-1443907	None:intergenic	30.0%
!!!	TGTCATTATGATTTTGGCTT+TGG	-	chr8.2:1444105-1444124	MS.gene68956:CDS	30.0%
	AAGACATTACTTGGAAGACT+TGG	-	chr8.2:1445171-1445190	MS.gene68956:intron	35.0%
	ACCTTTCCTTTGAATCTCTT+TGG	+	chr8.2:1443438-1443457	None:intergenic	35.0%
	ACTACACAAAGATGGAAGAA+AGG	+	chr8.2:1444485-1444504	None:intergenic	35.0%
	ATAACAACAGAAGAACCCTT+CGG	+	chr8.2:1444566-1444585	None:intergenic	35.0%
	ATCAACACACTACACAAAGA+TGG	+	chr8.2:1444493-1444512	None:intergenic	35.0%
	ATGTCTCAAATGCTCTACAA+AGG	-	chr8.2:1443799-1443818	MS.gene68956:intron	35.0%
	CATTATCTTTGTCTACGTGT+TGG	-	chr8.2:1445054-1445073	MS.gene68956:intron	35.0%
	CTACACAAAGATGGAAGAAA+GGG	+	chr8.2:1444484-1444503	None:intergenic	35.0%
	CTGGACAGTATGTATGTTAT+TGG	-	chr8.2:1444019-1444038	MS.gene68956:intron	35.0%
	CTTGAAGCAAAGACATTACT+TGG	-	chr8.2:1445162-1445181	MS.gene68956:intron	35.0%
	GATGATGCGTCATATATAAC+AGG	-	chr8.2:1445237-1445256	MS.gene68956:CDS	35.0%
	GGAAAGAGGAAAAACCTATT+TGG	+	chr8.2:1444892-1444911	None:intergenic	35.0%
	GGAGAAACTATAGTAGTTTC+CGG	-	chr8.2:1445258-1445277	MS.gene68956:CDS	35.0%
	GGTGTCTGATAATTATACAC+AGG	-	chr8.2:1444523-1444542	MS.gene68956:intron	35.0%
	TAAATATAATGTGTAGGCCC+TGG	-	chr8.2:1444755-1444774	MS.gene68956:intron	35.0%
	TCCAAAGAGATTCAAAGGAA+AGG	-	chr8.2:1443434-1443453	MS.gene68956:CDS	35.0%
	TGAGATTTGATGTCTTATCC+AGG	-	chr8.2:1443973-1443992	MS.gene68956:intron	35.0%
	TGGCATATTGAAGGAACAAT+TGG	+	chr8.2:1444689-1444708	None:intergenic	35.0%
	TTTGATTGACAAAACTGTCC+AGG	-	chr8.2:1443827-1443846	MS.gene68956:intron	35.0%
!	ACACAGTTTACACGAGTTTT+AGG	+	chr8.2:1444795-1444814	None:intergenic	35.0%
!	CACAGTTTACACGAGTTTTA+GGG	+	chr8.2:1444794-1444813	None:intergenic	35.0%
!	CTTTCAATCTAAAGTGTCAG+TGG	+	chr8.2:1444953-1444972	None:intergenic	35.0%
!	TACCTTCAGAAGAAGTATAG+TGG	+	chr8.2:1444347-1444366	None:intergenic	35.0%
!	TTATCTCCCATAACTTGTCA+AGG	+	chr8.2:1444315-1444334	None:intergenic	35.0%
!	TTTGTAGAGCATTTGAGACA+TGG	+	chr8.2:1443800-1443819	None:intergenic	35.0%
!!	ACAGTTTACACGAGTTTTAG+GGG	+	chr8.2:1444793-1444812	None:intergenic	35.0%
!!	GATATATGAAGCACTGATAC+TGG	+	chr8.2:1445094-1445113	None:intergenic	35.0%
!!	TTGTGTCTGAATGGATTTTG+TGG	-	chr8.2:1445116-1445135	MS.gene68956:intron	35.0%
!!	TTTGGCTTGTTACAGAAGTA+TGG	-	chr8.2:1444214-1444233	MS.gene68956:intron	35.0%
	ACTCACCATAGCAGAATTAC+TGG	+	chr8.2:1444859-1444878	None:intergenic	40.0%
	AGAGAAGATGAATTCACACC+TGG	+	chr8.2:1443848-1443867	None:intergenic	40.0%
	AGCCACTATACTTCTTCTGA+AGG	-	chr8.2:1444342-1444361	MS.gene68956:intron	40.0%
	ATGTAGCTGCTGAAAAACTG+AGG	-	chr8.2:1443742-1443761	MS.gene68956:intron	40.0%
	ATTCGGTCCTTGACAAGTTA+TGG	-	chr8.2:1444305-1444324	MS.gene68956:intron	40.0%
	CATCACCAGTAATTCTGCTA+TGG	-	chr8.2:1444851-1444870	MS.gene68956:intron	40.0%
	CATTCTGCAAACCCAAGATT+CGG	-	chr8.2:1444288-1444307	MS.gene68956:intron	40.0%
	CTCAAATGCTCTACAAAGGA+AGG	-	chr8.2:1443803-1443822	MS.gene68956:intron	40.0%
	GAAACTATAGTAGTTTCCGG+TGG	-	chr8.2:1445261-1445280	MS.gene68956:CDS	40.0%
	GTTATCATTTCATCGATCGC+TGG	-	chr8.2:1444581-1444600	MS.gene68956:intron	40.0%
	TAACATACATACTGTCCAGC+TGG	+	chr8.2:1444018-1444037	None:intergenic	40.0%
	TAGCAAATGATACACAACGC+AGG	+	chr8.2:1444923-1444942	None:intergenic	40.0%
	TGCAGAATGGAATCAACAGA+AGG	+	chr8.2:1444277-1444296	None:intergenic	40.0%
	TGTAGCTGCTGAAAAACTGA+GGG	-	chr8.2:1443743-1443762	MS.gene68956:intron	40.0%
	TTCGGTCCTTGACAAGTTAT+GGG	-	chr8.2:1444306-1444325	MS.gene68956:intron	40.0%
	TTTACCATTGCTGAGAGACT+TGG	-	chr8.2:1443492-1443511	MS.gene68956:CDS	40.0%
!	TGGTCATTAGTGTTACTGCT+AGG	+	chr8.2:1443935-1443954	None:intergenic	40.0%
	ACATAGCCATACTAGCTGGT+GGG	+	chr8.2:1444613-1444632	None:intergenic	45.0%
	ACTGCTTCAACACAGGGAAT+TGG	-	chr8.2:1443468-1443487	MS.gene68956:CDS	45.0%
	ACTTGGTACAACCGAAGACA+TGG	-	chr8.2:1445188-1445207	MS.gene68956:CDS	45.0%
	ACTTGTCAAGGACCGAATCT+TGG	+	chr8.2:1444303-1444322	None:intergenic	45.0%
	AGAAGGATTTGCAGCAGCAT+TGG	+	chr8.2:1444260-1444279	None:intergenic	45.0%
	ATCGTGACTGCTTCAACACA+GGG	-	chr8.2:1443462-1443481	MS.gene68956:CDS	45.0%
	CAAACCAAGTCTCTCAGCAA+TGG	+	chr8.2:1443499-1443518	None:intergenic	45.0%
	CCAGCTAGTATGGCTATGTA+TGG	-	chr8.2:1444614-1444633	MS.gene68956:intron	45.0%
	CCATACATAGCCATACTAGC+TGG	+	chr8.2:1444617-1444636	None:intergenic	45.0%
	CGAATCTTGGGTTTGCAGAA+TGG	+	chr8.2:1444290-1444309	None:intergenic	45.0%
	CTTGGTACAACCGAAGACAT+GGG	-	chr8.2:1445189-1445208	MS.gene68956:CDS	45.0%
	CTTGTCAAGGACCGAATCTT+GGG	+	chr8.2:1444302-1444321	None:intergenic	45.0%
	GACTTTGGTAAGGCCAAGAA+GGG	+	chr8.2:1444654-1444673	None:intergenic	45.0%
	GTAACCAAAACAGCCCTTCT+TGG	-	chr8.2:1444638-1444657	MS.gene68956:intron	45.0%
	TACATAGCCATACTAGCTGG+TGG	+	chr8.2:1444614-1444633	None:intergenic	45.0%
	TGTTATCTCTTCTCGCAGAC+AGG	-	chr8.2:1443533-1443552	MS.gene68956:CDS	45.0%
	TTATCACCCACCAGCTAGTA+TGG	-	chr8.2:1444604-1444623	MS.gene68956:intron	45.0%
!	AAGAACTGGACTTGGTAACC+TGG	+	chr8.2:1443994-1444013	None:intergenic	45.0%
!	CATTGCTGAGAGACTTGGTT+TGG	-	chr8.2:1443497-1443516	MS.gene68956:CDS	45.0%
!!	CTTCTTGGCCTTACCAAAGT+CGG	-	chr8.2:1444653-1444672	MS.gene68956:intron	45.0%
	ACTGTCCAGCTGGTAAGAAC+TGG	+	chr8.2:1444008-1444027	None:intergenic	50.0%
	CAAGTCCAGTTCTTACCAGC+TGG	-	chr8.2:1444000-1444019	MS.gene68956:intron	50.0%
	CAGCTGGTAAGAACTGGACT+TGG	+	chr8.2:1444002-1444021	None:intergenic	50.0%
	CGACTTTGGTAAGGCCAAGA+AGG	+	chr8.2:1444655-1444674	None:intergenic	50.0%
	GAAGAACCCTTCGGCAAGTA+TGG	+	chr8.2:1444557-1444576	None:intergenic	50.0%
	GATCGTGACTGCTTCAACAC+AGG	-	chr8.2:1443461-1443480	MS.gene68956:CDS	50.0%
	GCTGAAAAACTGAGGGCCAA+AGG	-	chr8.2:1443750-1443769	MS.gene68956:intron	50.0%
	TACACAACGCAGGAGATTCG+AGG	+	chr8.2:1444913-1444932	None:intergenic	50.0%
	TGTAGCAGCACCCATGTCTT+CGG	+	chr8.2:1445202-1445221	None:intergenic	50.0%
!	AAGGCCAAGAAGGGCTGTTT+TGG	+	chr8.2:1444645-1444664	None:intergenic	50.0%
!	CGTGTAAACTGTGTTGCTCC+TGG	-	chr8.2:1444801-1444820	MS.gene68956:intron	50.0%
!!	GCTGAGAGACTTGGTTTGGA+AGG	-	chr8.2:1443501-1443520	MS.gene68956:CDS	50.0%
!!	GGCAAGTACCGACTTTGGTA+AGG	+	chr8.2:1444664-1444683	None:intergenic	50.0%
!!	TGGAAGGCAAGTACCGACTT+TGG	+	chr8.2:1444669-1444688	None:intergenic	50.0%
!!!	GGGTGCTGCTACAGCTTTTT+TGG	-	chr8.2:1445209-1445228	MS.gene68956:CDS	50.0%
	CAGTCTCGATGGCATTCCAC+CGG	+	chr8.2:1445280-1445299	None:intergenic	55.0%
	CGCAGGAGATTCGAGGAAAG+AGG	+	chr8.2:1444906-1444925	None:intergenic	55.0%
	GCAGCTCCATACTTGCCGAA+GGG	-	chr8.2:1444548-1444567	MS.gene68956:intron	55.0%
	TGCAGCTCCATACTTGCCGA+AGG	-	chr8.2:1444547-1444566	MS.gene68956:intron	55.0%
	AGGGGCCATCTCAGCAGCCA+GGG	+	chr8.2:1444775-1444794	None:intergenic	65.0%
	GTAGGCCCTGGCTGCTGAGA+TGG	-	chr8.2:1444767-1444786	MS.gene68956:intron	65.0%
	TAGGGGCCATCTCAGCAGCC+AGG	+	chr8.2:1444776-1444795	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	1443426	1445302	1443426	ID=MS.gene68956
chr8.2	mRNA	1443426	1445302	1443426	ID=MS.gene68956.t1;Parent=MS.gene68956
chr8.2	exon	1445174	1445302	1445174	ID=MS.gene68956.t1.exon1;Parent=MS.gene68956.t1
chr8.2	CDS	1445174	1445302	1445174	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1
chr8.2	exon	1444880	1444996	1444880	ID=MS.gene68956.t1.exon2;Parent=MS.gene68956.t1
chr8.2	CDS	1444880	1444996	1444880	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1
chr8.2	exon	1444365	1444499	1444365	ID=MS.gene68956.t1.exon3;Parent=MS.gene68956.t1
chr8.2	CDS	1444365	1444499	1444365	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1
chr8.2	exon	1444058	1444183	1444058	ID=MS.gene68956.t1.exon4;Parent=MS.gene68956.t1
chr8.2	CDS	1444058	1444183	1444058	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1
chr8.2	exon	1443856	1443957	1443856	ID=MS.gene68956.t1.exon5;Parent=MS.gene68956.t1
chr8.2	CDS	1443856	1443957	1443856	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1
chr8.2	exon	1443426	1443575	1443426	ID=MS.gene68956.t1.exon6;Parent=MS.gene68956.t1
chr8.2	CDS	1443426	1443575	1443426	ID=cds.MS.gene68956.t1;Parent=MS.gene68956.t1

Gene Sequence

>MS.gene68956

ATGGAAATTCCAAAGAGATTCAAAGGAAAGGTTGCGATCGTGACTGCTTCAACACAGGGAATTGGTTTTACCATTGCTGAGAGACTTGGTTTGGAAGGTGCTTCTGTTGTTATCTCTTCTCGCAGACAGGTTTTCTTCCTTTTTCTTATTTGATTCATTCATTCATATTTCTCTCAGAAAGTATGTAAATTATTGTATGAGAATTGGTTAGAGAAAAGTTAATTGTTCAAACAAGTGATTTATCATGTATTAATATCATAATTGATTAATCCATATTATTTTTTTAATTTGGTTGAATCGCTGCAGAAAAATGTTGATGTAGCTGCTGAAAAACTGAGGGCCAAAGGAATTGATGTATTTGCAGTTGTTTGCCATGTCTCAAATGCTCTACAAAGGAAGGATTTGATTGACAAAACTGTCCAGGTGTGAATTCATCTTCTCTTGTGAATGTGATCTTGTCCAATTTCAATACAAGACTTGTGTCGATTTTATCTTTGCATTCAGTTCCTAGCAGTAACACTAATGACCATTTTACAACTATCCTATATGAGATTTGATGTCTTATCCAGGTTACCAAGTCCAGTTCTTACCAGCTGGACAGTATGTATGTTATTGGAATCTTAACACCCATCATTCATATTTTGTACAAGTTTAAATAGAATTGGCGGACGATATGTTTTGTCATTATGATTTTGGCTTTGGTTTTTTCAGTGTAGTTTAATAATCTTTCATATTATAGTCTGTCTTTCTCACTATTTGAATGGAGTTCTAATATGTGTTTGAATTTTTTTGGCTTGTTACAGAAGTATGGAAAGATAGATGTTGTTGTGTCCAATGCTGCTGCAAATCCTTCTGTTGATTCCATTCTGCAAACCCAAGATTCGGTCCTTGACAAGTTATGGGAGATAAATGTCAAAGCCACTATACTTCTTCTGAAGGTAATTAAGCTGTGAAAATGGCTTTAACAAATAAAAGAAAAGAAATAATCCAATATATATATATATATATATATATATATATATATATATATATATATATATATGTTTCAGTGTTTTCCCTTTCTTCCATCTTTGTGTAGTGTGTTGATGTAGTAAAATGGTGTCTGATAATTATACACAGGATGCAGCTCCATACTTGCCGAAGGGTTCTTCTGTTGTTATCATTTCATCGATCGCTGGTTATCACCCACCAGCTAGTATGGCTATGTATGGAGTAACCAAAACAGCCCTTCTTGGCCTTACCAAAGTCGGTACTTGCCTTCCAATTGTTCCTTCAATATGCCATATGAATTGTTACTGTTTTTTTTTGTTATATCTGATAATTATTATGTAAATATAATGTGTAGGCCCTGGCTGCTGAGATGGCCCCTAAAACTCGTGTAAACTGTGTTGCTCCTGGTTTTGTACCAACTAATTTTGCTTCATTCATCACCAGTAATTCTGCTATGGTGAGTATCTTTTTAACCAAATAGGTTTTTCCTCTTTCCTCGAATCTCCTGCGTTGTGTATCATTTGCTATATAACACCACTGACACTTTAGATTGAAAGCGTGTTTGAGTGTCTGATACATGTCAATGTTCGAGACAGACACATATATGATTGCATACACTCACTTTTATTTTTTCAAATCATTATCTTTGTCTACGTGTTGGTGTCAATATTGTGTCCAGTATCAGTGCTTCATATATCATTTGTGTCTGAATGGATTTTGTGGCTATATTATTGTAGAGAGAAGAGCTTGAAGCAAAGACATTACTTGGAAGACTTGGTACAACCGAAGACATGGGTGCTGCTACAGCTTTTTTGGCATCTGATGATGCGTCATATATAACAGGAGAAACTATAGTAGTTTCCGGTGGAATGCCATCGAGACTGTAG

Protein sequence

>MS.gene68956.t1

MEIPKRFKGKVAIVTASTQGIGFTIAERLGLEGASVVISSRRQKNVDVAAEKLRAKGIDVFAVVCHVSNALQRKDLIDKTVQKYGKIDVVVSNAAANPSVDSILQTQDSVLDKLWEINVKATILLLKDAAPYLPKGSSVVIISSIAGYHPPASMAMYGVTKTALLGLTKALAAEMAPKTRVNCVAPGFVPTNFASFITSNSAMREELEAKTLLGRLGTTEDMGAATAFLASDDASYITGETIVVSGGMPSRL