AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62194

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62194.t1 MTR_3g068070 95.911 269 11 0 1 269 1 269 0.0 533
MS.gene62194.t1 MTR_3g068085 88.258 264 31 0 1 264 1 264 5.07e-168 466
MS.gene62194.t1 MTR_3g068065 84.000 250 40 0 15 264 17 266 2.64e-157 439
MS.gene62194.t1 MTR_3g068155 74.517 259 66 0 6 264 10 268 1.62e-146 411
MS.gene62194.t1 MTR_3g068175 70.611 262 76 1 4 264 5 266 4.63e-139 392
MS.gene62194.t1 MTR_3g068175 69.549 266 76 2 4 264 5 270 6.60e-137 387
MS.gene62194.t1 MTR_4g064883 62.598 254 95 0 12 265 11 264 1.81e-125 358
MS.gene62194.t1 MTR_4g064887 58.779 262 108 0 1 262 1 262 2.14e-117 337
MS.gene62194.t1 MTR_7g063320 58.113 265 107 1 1 261 1 265 7.24e-111 320
MS.gene62194.t1 MTR_7g063270 58.397 262 106 1 2 260 3 264 2.36e-107 311
MS.gene62194.t1 MTR_7g063260 58.175 263 106 1 2 260 3 265 7.44e-107 310
MS.gene62194.t1 MTR_7g063330 57.034 263 110 1 1 260 1 263 6.75e-106 308
MS.gene62194.t1 MTR_7g063390 56.439 264 111 2 2 261 3 266 1.19e-105 307
MS.gene62194.t1 MTR_7g063360 57.576 264 108 2 2 261 3 266 2.02e-105 307
MS.gene62194.t1 MTR_4g064883 63.636 198 72 0 12 209 11 208 1.85e-96 282
MS.gene62194.t1 MTR_4g064887 59.330 209 85 0 1 209 1 209 3.03e-91 269
MS.gene62194.t1 MTR_7g063270 48.473 262 83 3 2 260 3 215 5.73e-78 235
MS.gene62194.t1 MTR_4g132850 33.740 246 158 4 15 259 7 248 3.29e-36 129
MS.gene62194.t1 MTR_7g094510 32.806 253 158 7 15 259 6 254 1.22e-31 117
MS.gene62194.t1 MTR_7g094520 32.812 256 154 8 15 259 11 259 1.42e-30 115
MS.gene62194.t1 MTR_3g085740 31.707 246 157 6 21 261 81 320 2.13e-29 113
MS.gene62194.t1 MTR_7g071680 31.636 275 150 8 15 260 29 294 5.84e-28 109
MS.gene62194.t1 MTR_4g058810 31.418 261 166 9 14 264 10 267 3.73e-27 106
MS.gene62194.t1 MTR_4g058840 30.769 260 167 9 15 264 11 267 2.24e-25 101
MS.gene62194.t1 MTR_4g058820 30.769 260 167 9 15 264 11 267 2.24e-25 101
MS.gene62194.t1 MTR_7g083870 29.961 257 165 7 15 260 35 287 2.21e-24 99.8
MS.gene62194.t1 MTR_1196s0010 32.114 246 154 6 18 259 277 513 2.85e-24 101
MS.gene62194.t1 MTR_4g078630 29.771 262 159 7 15 260 19 271 1.87e-22 94.0
MS.gene62194.t1 MTR_4g052350 31.034 261 168 9 15 269 14 268 8.50e-22 92.0
MS.gene62194.t1 MTR_7g107670 28.352 261 171 7 11 260 29 284 1.05e-21 92.4
MS.gene62194.t1 MTR_4g052340 29.963 267 156 9 15 265 14 265 1.10e-21 91.7
MS.gene62194.t1 MTR_4g052320 29.297 256 171 7 15 264 8 259 1.73e-21 91.3
MS.gene62194.t1 MTR_4g131740 31.203 266 153 8 15 262 40 293 5.65e-20 87.4
MS.gene62194.t1 MTR_4g052300 30.651 261 160 9 15 264 8 258 8.56e-20 86.7
MS.gene62194.t1 MTR_8g442750 27.344 256 171 6 15 259 14 265 1.77e-19 86.3
MS.gene62194.t1 MTR_1g068955 30.000 260 165 9 15 263 14 267 3.39e-19 85.1
MS.gene62194.t1 MTR_3g020670 30.000 260 163 9 15 260 16 270 4.48e-19 84.7
MS.gene62194.t1 MTR_1g051165 27.652 264 173 12 15 263 14 274 1.16e-17 80.9
MS.gene62194.t1 MTR_4g058830 32.558 172 109 6 98 264 44 213 1.64e-17 79.3
MS.gene62194.t1 MTR_7g105590 29.570 186 124 4 15 197 8 189 2.48e-17 78.2
MS.gene62194.t1 MTR_6g023990 27.675 271 171 11 1 259 5 262 9.85e-17 78.2
MS.gene62194.t1 MTR_7g105580 25.954 262 170 5 8 265 1 242 1.04e-16 77.8
MS.gene62194.t1 MTR_3g068075 74.000 50 12 1 123 171 1 50 1.04e-16 73.6
MS.gene62194.t1 MTR_7g105460 28.137 263 177 6 6 261 4 261 2.58e-16 77.0
MS.gene62194.t1 MTR_6g023610 28.358 268 170 10 5 259 3 261 4.52e-16 76.3
MS.gene62194.t1 MTR_7g110140 30.888 259 165 9 11 261 2 254 6.14e-16 75.9
MS.gene62194.t1 MTR_6g023590 26.740 273 168 11 5 259 3 261 6.33e-16 75.9
MS.gene62194.t1 MTR_1g082760 28.682 258 171 7 15 263 40 293 6.69e-16 76.3
MS.gene62194.t1 MTR_8g089575 31.313 198 127 5 17 207 47 242 1.53e-15 75.5
MS.gene62194.t1 MTR_7g110130 27.170 265 168 8 11 261 2 255 3.04e-15 73.9
MS.gene62194.t1 MTR_2g090165 27.586 261 171 8 11 261 2 254 5.94e-15 73.2
MS.gene62194.t1 MTR_7g110160 28.846 260 151 8 11 261 2 236 2.71e-14 70.9
MS.gene62194.t1 MTR_6g023600 27.473 273 166 10 5 259 3 261 3.37e-14 70.9
MS.gene62194.t1 MTR_4g134660 32.948 173 107 4 18 184 9 178 1.05e-13 69.3
MS.gene62194.t1 MTR_8g044240 28.821 229 144 8 15 229 69 292 2.53e-13 69.3
MS.gene62194.t1 MTR_6g041980 32.515 163 91 5 11 168 60 208 5.27e-13 67.8
MS.gene62194.t1 MTR_7g105570 29.050 179 110 4 94 261 46 218 8.82e-13 66.6
MS.gene62194.t1 MTR_3g113870 26.667 180 126 3 28 205 34 209 1.76e-12 65.9
MS.gene62194.t1 MTR_6g041980 32.515 163 91 5 11 168 60 208 1.82e-12 66.6
MS.gene62194.t1 MTR_6g023600 27.106 273 161 10 5 259 3 255 6.23e-12 64.3
MS.gene62194.t1 MTR_1275s0010 28.205 117 84 0 139 255 259 375 1.66e-11 64.3
MS.gene62194.t1 MTR_3g087850 25.316 237 150 6 21 237 32 261 5.50e-11 62.0
MS.gene62194.t1 MTR_3g098470 33.333 144 93 3 12 154 29 170 5.61e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62194.t1 AT5G06060 70.155 258 77 0 12 269 6 263 7.51e-139 391
MS.gene62194.t1 AT2G29260 63.241 253 93 0 12 264 65 317 1.68e-119 345
MS.gene62194.t1 AT2G29360 61.303 261 101 0 5 265 6 266 7.90e-116 333
MS.gene62194.t1 AT2G29290 60.630 254 100 0 12 265 4 257 1.63e-114 330
MS.gene62194.t1 AT1G07450 60.078 258 102 1 8 265 1 257 2.25e-114 329
MS.gene62194.t1 AT2G29150 61.303 261 100 1 5 265 6 265 3.08e-113 327
MS.gene62194.t1 AT2G29350 61.961 255 96 1 11 265 11 264 6.35e-113 326
MS.gene62194.t1 AT1G07440 59.843 254 101 1 12 265 9 261 3.76e-112 324
MS.gene62194.t1 AT2G29330 60.392 255 99 2 12 265 4 257 2.05e-109 317
MS.gene62194.t1 AT2G30670 58.661 254 105 0 12 265 4 257 1.52e-108 315
MS.gene62194.t1 AT2G29370 56.391 266 115 1 1 265 1 266 3.25e-108 314
MS.gene62194.t1 AT2G29340 57.087 254 109 0 12 265 4 257 9.97e-107 310
MS.gene62194.t1 AT2G29340 57.087 254 109 0 12 265 4 257 4.20e-106 310
MS.gene62194.t1 AT2G29310 57.874 254 107 0 12 265 4 257 1.44e-105 307
MS.gene62194.t1 AT2G29320 56.757 259 111 1 8 265 6 264 3.88e-104 304
MS.gene62194.t1 AT2G29290 59.402 234 95 0 32 265 7 240 8.52e-102 297
MS.gene62194.t1 AT2G29300 55.686 255 112 1 12 265 4 258 2.86e-101 296
MS.gene62194.t1 AT2G29300 55.200 250 111 1 12 260 4 253 1.23e-97 288
MS.gene62194.t1 AT2G29350 60.619 226 88 1 40 265 2 226 9.71e-97 283
MS.gene62194.t1 AT2G29320 56.780 236 101 1 31 265 11 246 4.38e-94 278
MS.gene62194.t1 AT2G29310 57.021 235 101 0 31 265 21 255 4.86e-94 278
MS.gene62194.t1 AT2G29350 63.402 194 71 0 11 204 11 204 1.36e-86 258
MS.gene62194.t1 AT2G29310 57.576 198 84 0 12 209 4 201 2.61e-78 236
MS.gene62194.t1 AT2G29340 55.556 198 88 0 12 209 4 201 1.53e-77 234
MS.gene62194.t1 AT2G29340 55.556 198 88 0 12 209 4 201 1.53e-77 234
MS.gene62194.t1 AT2G29320 55.838 197 86 1 8 203 6 202 7.41e-76 229
MS.gene62194.t1 AT1G07440 60.248 161 64 0 12 172 9 169 1.83e-69 212
MS.gene62194.t1 AT2G29310 56.425 179 78 0 31 209 21 199 1.67e-66 206
MS.gene62194.t1 AT2G29170 58.824 102 41 1 1 101 1 102 9.35e-35 121
MS.gene62194.t1 AT4G05530 34.818 247 154 7 15 259 9 250 8.57e-34 123
MS.gene62194.t1 AT5G18210 33.585 265 157 9 8 259 1 259 1.43e-33 123
MS.gene62194.t1 AT3G03980 33.457 269 160 9 6 259 5 269 7.20e-33 121
MS.gene62194.t1 AT4G13180 33.210 271 163 10 1 261 1 263 3.49e-31 117
MS.gene62194.t1 AT5G18210 33.456 272 148 11 8 266 1 252 4.34e-30 114
MS.gene62194.t1 AT1G24360 30.769 247 158 8 21 261 80 319 2.89e-27 107
MS.gene62194.t1 AT3G04000 32.210 267 165 8 6 258 5 269 1.45e-26 105
MS.gene62194.t1 AT3G55310 29.787 282 180 13 1 267 1 279 3.69e-25 101
MS.gene62194.t1 AT3G55290 30.483 269 165 12 15 267 17 279 1.29e-24 99.8
MS.gene62194.t1 AT3G55290 30.483 269 165 12 15 267 18 280 1.51e-24 99.8
MS.gene62194.t1 AT3G46170 29.457 258 171 8 15 263 26 281 3.62e-24 99.0
MS.gene62194.t1 AT3G51680 29.643 280 173 8 1 260 20 295 7.53e-24 98.2
MS.gene62194.t1 AT2G17845 29.389 262 171 7 15 264 47 306 2.50e-23 97.1
MS.gene62194.t1 AT3G26760 28.244 262 165 7 15 260 36 290 3.07e-23 96.7
MS.gene62194.t1 AT1G63380 28.369 282 185 11 1 267 1 280 6.90e-23 95.1
MS.gene62194.t1 AT1G63380 28.947 266 174 9 15 267 19 282 7.82e-23 95.1
MS.gene62194.t1 AT1G63380 28.947 266 174 9 15 267 15 278 9.45e-23 94.7
MS.gene62194.t1 AT1G54870 32.576 264 153 8 15 263 82 335 1.15e-22 95.5
MS.gene62194.t1 AT1G54870 32.576 264 153 8 15 263 84 337 1.23e-22 95.5
MS.gene62194.t1 AT1G63380 28.571 259 173 8 15 263 22 278 1.34e-22 94.7
MS.gene62194.t1 AT1G52340 30.682 264 159 8 15 260 18 275 2.61e-22 94.0
MS.gene62194.t1 AT2G47130 30.651 261 166 9 8 261 1 253 3.57e-22 92.8
MS.gene62194.t1 AT1G62610 27.413 259 176 8 15 263 13 269 5.04e-22 92.8
MS.gene62194.t1 AT1G62610 27.820 266 177 9 15 267 15 278 5.21e-22 92.8
MS.gene62194.t1 AT1G62610 27.820 266 177 9 15 267 14 277 5.79e-22 92.8
MS.gene62194.t1 AT1G62610 27.820 266 177 9 15 267 19 282 6.03e-22 92.8
MS.gene62194.t1 AT2G47120 29.008 262 168 10 8 261 1 252 2.45e-20 87.8
MS.gene62194.t1 AT3G05260 32.685 257 159 7 15 262 37 288 5.09e-20 87.8
MS.gene62194.t1 AT2G47120 28.627 255 166 9 15 261 7 253 6.70e-20 86.7
MS.gene62194.t1 AT3G42960 30.116 259 157 9 18 260 11 261 3.34e-19 85.1
MS.gene62194.t1 AT4G03140 26.996 263 164 7 15 260 78 329 3.44e-19 86.3
MS.gene62194.t1 AT3G12800 25.000 256 178 5 15 259 10 262 4.81e-19 85.1
MS.gene62194.t1 AT3G29260 29.771 262 167 9 8 261 1 253 1.60e-17 80.1
MS.gene62194.t1 AT3G26770 26.966 267 174 7 15 267 41 300 5.57e-17 79.3
MS.gene62194.t1 AT2G47140 28.897 263 169 11 8 261 1 254 2.65e-16 76.6
MS.gene62194.t1 AT3G29250 28.517 263 174 8 7 261 36 292 4.99e-16 76.6
MS.gene62194.t1 AT3G29250 28.626 262 171 9 8 261 1 254 8.03e-15 72.8
MS.gene62194.t1 AT5G04900 30.493 223 135 9 21 229 83 299 9.20e-15 73.6
MS.gene62194.t1 AT3G29250 28.517 263 172 9 7 261 36 290 1.04e-14 72.8
MS.gene62194.t1 AT4G13250 26.641 259 151 8 18 243 162 414 4.02e-14 72.4
MS.gene62194.t1 AT1G10310 32.143 168 111 3 18 184 18 183 4.51e-14 70.5
MS.gene62194.t1 AT3G47350 29.534 193 125 6 17 204 46 232 5.47e-14 71.2
MS.gene62194.t1 AT3G47350 29.534 193 125 6 17 204 46 232 7.23e-14 70.5
MS.gene62194.t1 AT1G24470 28.079 203 137 6 17 213 52 251 9.49e-14 70.5
MS.gene62194.t1 AT3G03330 29.016 193 128 5 12 197 38 228 4.80e-13 68.6
MS.gene62194.t1 AT5G65205 29.444 180 116 6 21 197 12 183 7.94e-13 67.4
MS.gene62194.t1 AT5G10050 26.702 191 133 3 21 210 11 195 1.49e-12 66.6
MS.gene62194.t1 AT3G06060 26.733 202 141 2 9 207 31 228 3.74e-12 65.9
MS.gene62194.t1 AT4G09750 33.544 158 86 5 15 167 41 184 4.02e-12 65.9
MS.gene62194.t1 AT3G06060 26.733 202 141 2 9 207 48 245 4.46e-12 65.9
MS.gene62194.t1 AT1G49670 29.439 214 124 7 17 211 6 211 8.93e-12 65.5
MS.gene62194.t1 AT1G49670 29.439 214 124 7 17 211 6 211 9.49e-12 65.5
MS.gene62194.t1 AT3G47360 30.208 192 123 6 18 204 48 233 1.35e-11 63.9
MS.gene62194.t1 AT1G67730 27.638 199 131 7 17 206 51 245 1.96e-11 63.5
MS.gene62194.t1 AT4G20760 30.317 221 124 9 16 211 62 277 5.14e-11 62.4
MS.gene62194.t1 AT4G20760 30.317 221 124 9 16 211 42 257 5.50e-11 62.4

Find 47 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTCTTCTTAAAGAATC+TGG 0.311673 3.2:-62757468 MS.gene62194:CDS
AATGGGCAAAAGACAATATA+AGG 0.317797 3.2:-62757231 MS.gene62194:CDS
GTTTCTGCTATACGCTTTAT+CGG 0.318496 3.2:+62756699 None:intergenic
TGCCTCTCATGCTGCTCTCT+TGG 0.339487 3.2:+62758670 None:intergenic
GGAAAGTCTGCTCTAGTTAC+TGG 0.355585 3.2:-62758630 MS.gene62194:CDS
GGACAAACTGTTTCTGTTGA+TGG 0.363679 3.2:-62756618 MS.gene62194:CDS
CTTCTTAAAGAATCTGGAAA+TGG 0.409640 3.2:-62757462 MS.gene62194:CDS
TCGGCTGTATACTCAATTGT+TGG 0.433889 3.2:+62757552 None:intergenic
ACTCACTGCATAGATGCTTC+CGG 0.439791 3.2:+62757392 None:intergenic
TGCATTCCATCTATGTCAAC+TGG 0.450264 3.2:-62757493 MS.gene62194:CDS
GATGGAGGATTTACTGTGAA+TGG 0.451398 3.2:-62756600 MS.gene62194:CDS
AAAAGTGATGACTACTAACT+TGG 0.463013 3.2:-62757520 MS.gene62194:CDS
ACTGGTGGAACTCGTGGAAT+CGG 0.476543 3.2:-62758612 MS.gene62194:CDS
GTGGAGGAACTAGCTGAGTT+TGG 0.479590 3.2:-62758579 MS.gene62194:CDS
TTTCCAGATTCTTTAAGAAG+AGG 0.503369 3.2:+62757465 None:intergenic
AACCAAGAGAGCAGCATGAG+AGG 0.505029 3.2:-62758672 MS.gene62194:CDS
CCAGCTGCTTCCTACATCAC+TGG 0.520639 3.2:-62756639 MS.gene62194:CDS
CAAGTTGCTTCTGCTTTCAA+CGG 0.524543 3.2:-62758420 MS.gene62194:CDS
CTTAAATGAGTGGAAAGAGA+AGG 0.530669 3.2:-62758508 MS.gene62194:CDS
AGCATCTATGCAGTGAGTAA+AGG 0.540433 3.2:-62757387 MS.gene62194:intron
CTAGTTACTGGTGGAACTCG+TGG 0.541240 3.2:-62758618 MS.gene62194:CDS
AGCTATTAGGTAAACAATGC+TGG 0.541729 3.2:-62757591 MS.gene62194:intron
TTAAATGAGTGGAAAGAGAA+GGG 0.543808 3.2:-62758507 MS.gene62194:CDS
AACAGTTTGTCCAGTGATGT+AGG 0.560729 3.2:+62756629 None:intergenic
AGGCACAAGATGGTCTCTCA+AGG 0.570614 3.2:-62758652 MS.gene62194:CDS
GATGTGCCAGTTGACATAGA+TGG 0.576163 3.2:+62757487 None:intergenic
CCACAGTGTACACTTGCTCT+AGG 0.579593 3.2:-62758554 MS.gene62194:CDS
AATCGGTCATGCTGTGGTGG+AGG 0.580717 3.2:-62758595 MS.gene62194:CDS
ATAAGCTCCTCTCTTTGAGA+AGG 0.588037 3.2:+62758447 None:intergenic
CCAGTGATGTAGGAAGCAGC+TGG 0.588298 3.2:+62756639 None:intergenic
ATCTTCTCCTTCTCAAAGAG+AGG 0.595566 3.2:-62758454 MS.gene62194:CDS
GCAGCATGAGAGGCACAAGA+TGG 0.597950 3.2:-62758662 MS.gene62194:CDS
TCTCTGATAAGCGTAGGTAC+CGG 0.606028 3.2:-62757411 MS.gene62194:CDS
AACACATGAAGTGTCTGCCT+TGG 0.606041 3.2:-62756679 MS.gene62194:CDS
TGGAATCGGTCATGCTGTGG+TGG 0.606109 3.2:-62758598 MS.gene62194:CDS
CAAACTGTTTCTGTTGATGG+AGG 0.607479 3.2:-62756615 MS.gene62194:CDS
AAGTCTGCTCTAGTTACTGG+TGG 0.609393 3.2:-62758627 MS.gene62194:CDS
AGGAAGCAGCTGGCAGACAA+AGG 0.611292 3.2:+62756649 None:intergenic
TGAGCAAATGCTTAAATGAG+TGG 0.614861 3.2:-62758518 MS.gene62194:CDS
GTTGCATCTCTGATAAGCGT+AGG 0.625038 3.2:-62757417 MS.gene62194:CDS
GGAGCAACTGTGTTGCACCC+TGG 0.628385 3.2:-62757210 MS.gene62194:CDS
TCGTGGAATCGGTCATGCTG+TGG 0.634799 3.2:-62758601 MS.gene62194:CDS
GGCACAAGATGGTCTCTCAA+GGG 0.644640 3.2:-62758651 MS.gene62194:CDS
ACAATGCTGGTACAAATGTG+AGG 0.662281 3.2:-62757578 MS.gene62194:CDS
GCTTATCAGAGATGCAACAG+AGG 0.677312 3.2:+62757422 None:intergenic
CAGACAAAGGAAAGTCACCA+AGG 0.716868 3.2:+62756662 None:intergenic
CCTAGAGCAAGTGTACACTG+TGG 0.805980 3.2:+62758554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATCAACATAATATAGAAA+CGG + chr3.2:62756910-62756929 None:intergenic 20.0%
!! GAAAACGAAATATATAGAAA+AGG + chr3.2:62756888-62756907 None:intergenic 20.0%
!! TTCATAAAGAAAAAATGACA+AGG + chr3.2:62758138-62758157 None:intergenic 20.0%
!!! TACTATTTTATTTTTTCACC+AGG + chr3.2:62757319-62757338 None:intergenic 20.0%
! ATAGTAGAAGTTATAGAAGA+AGG - chr3.2:62757333-62757352 MS.gene62194:intron 25.0%
! ATCTGTAGTATTAATCCAAA+TGG - chr3.2:62758440-62758459 MS.gene62194:CDS 25.0%
! CTAATGCATTATAACTCTAA+CGG - chr3.2:62757246-62757265 MS.gene62194:CDS 25.0%
! TATTATGTTCAGAACTTATG+TGG - chr3.2:62758466-62758485 MS.gene62194:CDS 25.0%
! TGTGCTAGTTGAAAATAATT+TGG + chr3.2:62757078-62757097 None:intergenic 25.0%
!! ATGTTATAAGATTTTCTTCC+TGG - chr3.2:62757298-62757317 MS.gene62194:intron 25.0%
!! CTTGTTATAAGACTTTCTTA+TGG - chr3.2:62757541-62757560 MS.gene62194:CDS 25.0%
!! TGATTTGTATGTTTGTCTTA+AGG - chr3.2:62757120-62757139 MS.gene62194:intron 25.0%
!!! CATCACTTTTGAATAATCTT+CGG + chr3.2:62757726-62757745 None:intergenic 25.0%
AAAAAGTCTTGCTTGTGAAT+GGG - chr3.2:62758008-62758027 MS.gene62194:intron 30.0%
AAAAGTGATGACTACTAACT+TGG - chr3.2:62757736-62757755 MS.gene62194:intron 30.0%
AACCGAAATCATGTATTAGT+AGG - chr3.2:62758297-62758316 MS.gene62194:intron 30.0%
AATGGGCAAAAGACAATATA+AGG - chr3.2:62758025-62758044 MS.gene62194:intron 30.0%
ACCTTTAATTATCCATTCTC+TGG - chr3.2:62758112-62758131 MS.gene62194:intron 30.0%
AGCCTACTAATACATGATTT+CGG + chr3.2:62758302-62758321 None:intergenic 30.0%
CAGCAAAAATTAAAATGCAG+AGG + chr3.2:62758381-62758400 None:intergenic 30.0%
CTGAACATAATATTGCCATT+TGG + chr3.2:62758458-62758477 None:intergenic 30.0%
CTTATAACAACCTTAGCATA+GGG + chr3.2:62757400-62757419 None:intergenic 30.0%
CTTCTTAAAGAATCTGGAAA+TGG - chr3.2:62757794-62757813 MS.gene62194:intron 30.0%
TGATAATCTTGTGAGCTATT+AGG - chr3.2:62757652-62757671 MS.gene62194:intron 30.0%
TTAAATGAGTGGAAAGAGAA+GGG - chr3.2:62756749-62756768 MS.gene62194:intron 30.0%
TTAGCAATAAACTGCAAGAA+AGG + chr3.2:62758503-62758522 None:intergenic 30.0%
! TGCATTCTTTTGATGTACTT+TGG - chr3.2:62757518-62757537 MS.gene62194:CDS 30.0%
! TTTCCAGATTCTTTAAGAAG+AGG + chr3.2:62757794-62757813 None:intergenic 30.0%
!! TTACTGTGAATGGATTTTCT+GGG - chr3.2:62758666-62758685 MS.gene62194:CDS 30.0%
!! TTTACTGTGAATGGATTTTC+TGG - chr3.2:62758665-62758684 MS.gene62194:CDS 30.0%
!!! GAGAAGGGTTTTTTTGTTTA+TGG - chr3.2:62756764-62756783 MS.gene62194:intron 30.0%
!!! GGGTTTTTTTGTTTATGGAT+CGG - chr3.2:62756769-62756788 MS.gene62194:intron 30.0%
AAATCAAGTGCGTGTAAGAT+TGG + chr3.2:62757467-62757486 None:intergenic 35.0%
AATCAATTTGTAGATGCTCG+TGG - chr3.2:62757015-62757034 MS.gene62194:intron 35.0%
AGCTATTAGGTAAACAATGC+TGG - chr3.2:62757665-62757684 MS.gene62194:intron 35.0%
CAAAAAGTCTTGCTTGTGAA+TGG - chr3.2:62758007-62758026 MS.gene62194:intron 35.0%
CATCCTCTTCTTAAAGAATC+TGG - chr3.2:62757788-62757807 MS.gene62194:intron 35.0%
CCTATGCTAAGGTTGTTATA+AGG - chr3.2:62757398-62757417 MS.gene62194:CDS 35.0%
CCTTATAACAACCTTAGCAT+AGG + chr3.2:62757401-62757420 None:intergenic 35.0%
CTTAAATGAGTGGAAAGAGA+AGG - chr3.2:62756748-62756767 MS.gene62194:intron 35.0%
GCCAGAGAATGGATAATTAA+AGG + chr3.2:62758116-62758135 None:intergenic 35.0%
GTATTATTGAATCGCAACGT+AGG + chr3.2:62758192-62758211 None:intergenic 35.0%
GTTTCTGCTATACGCTTTAT+CGG + chr3.2:62758560-62758579 None:intergenic 35.0%
TACCAGAAACAAGACGAAAA+AGG + chr3.2:62757595-62757614 None:intergenic 35.0%
TGAGCAAATGCTTAAATGAG+TGG - chr3.2:62756738-62756757 MS.gene62194:CDS 35.0%
! GATGTTGAATTTTGCATAGC+TGG - chr3.2:62756931-62756950 MS.gene62194:intron 35.0%
!! TAAAATGCAGAGGCATCATA+TGG + chr3.2:62758371-62758390 None:intergenic 35.0%
!!! AGTTCACTTTTTGAGTGTGT+TGG - chr3.2:62757215-62757234 MS.gene62194:CDS 35.0%
AAAATGACAAGGCCAGAGAA+TGG + chr3.2:62758127-62758146 None:intergenic 40.0%
AACAGTTTGTCCAGTGATGT+AGG + chr3.2:62758630-62758649 None:intergenic 40.0%
ACAATGCTGGTACAAATGTG+AGG - chr3.2:62757678-62757697 MS.gene62194:intron 40.0%
AGCATCTATGCAGTGAGTAA+AGG - chr3.2:62757869-62757888 MS.gene62194:intron 40.0%
ATCTTCTCCTTCTCAAAGAG+AGG - chr3.2:62756802-62756821 MS.gene62194:intron 40.0%
ATGCATACGAAACACGATCA+TGG - chr3.2:62758238-62758257 MS.gene62194:intron 40.0%
ATGCTCGTGGAAAACTTAGT+TGG - chr3.2:62757028-62757047 MS.gene62194:intron 40.0%
CAAGTTGCTTCTGCTTTCAA+CGG - chr3.2:62756836-62756855 MS.gene62194:intron 40.0%
GATGGAGGATTTACTGTGAA+TGG - chr3.2:62758656-62758675 MS.gene62194:CDS 40.0%
GTATACAAAAACGTCGCTTG+TGG - chr3.2:62758068-62758087 MS.gene62194:intron 40.0%
TCGGCTGTATACTCAATTGT+TGG + chr3.2:62757707-62757726 None:intergenic 40.0%
TGATTTCGGTTGCATGAATC+AGG + chr3.2:62758288-62758307 None:intergenic 40.0%
TGCAGTGAGTAAAGGTAACT+TGG - chr3.2:62757877-62757896 MS.gene62194:intron 40.0%
TGCATACGAAACACGATCAT+GGG - chr3.2:62758239-62758258 MS.gene62194:intron 40.0%
TGCATTCCATCTATGTCAAC+TGG - chr3.2:62757763-62757782 MS.gene62194:intron 40.0%
! AAGCGACGTTTTTGTATACC+AGG + chr3.2:62758067-62758086 None:intergenic 40.0%
! AGCGACGTTTTTGTATACCA+GGG + chr3.2:62758066-62758085 None:intergenic 40.0%
! ATAAGCTCCTCTCTTTGAGA+AGG + chr3.2:62756812-62756831 None:intergenic 40.0%
!! ACACGCACTTGATTTTCAAG+TGG - chr3.2:62757472-62757491 MS.gene62194:CDS 40.0%
!! CAAACTGTTTCTGTTGATGG+AGG - chr3.2:62758641-62758660 MS.gene62194:CDS 40.0%
!! GGACAAACTGTTTCTGTTGA+TGG - chr3.2:62758638-62758657 MS.gene62194:CDS 40.0%
!!! CACCTTTTTCGTCTTGTTTC+TGG - chr3.2:62757590-62757609 MS.gene62194:intron 40.0%
AAAAACGTCGCTTGTGGAAC+AGG - chr3.2:62758074-62758093 MS.gene62194:intron 45.0%
AAGTCTGCTCTAGTTACTGG+TGG - chr3.2:62756629-62756648 MS.gene62194:CDS 45.0%
ACTCACTGCATAGATGCTTC+CGG + chr3.2:62757867-62757886 None:intergenic 45.0%
AGATGCTCACACTGACACTA+CGG + chr3.2:62758424-62758443 None:intergenic 45.0%
CAGACAAAGGAAAGTCACCA+AGG + chr3.2:62758597-62758616 None:intergenic 45.0%
GATGTGCCAGTTGACATAGA+TGG + chr3.2:62757772-62757791 None:intergenic 45.0%
GCTTATCAGAGATGCAACAG+AGG + chr3.2:62757837-62757856 None:intergenic 45.0%
GGAAAGTCTGCTCTAGTTAC+TGG - chr3.2:62756626-62756645 MS.gene62194:CDS 45.0%
GTTGCATCTCTGATAAGCGT+AGG - chr3.2:62757839-62757858 MS.gene62194:intron 45.0%
TCTCTGATAAGCGTAGGTAC+CGG - chr3.2:62757845-62757864 MS.gene62194:intron 45.0%
! AACACATGAAGTGTCTGCCT+TGG - chr3.2:62758577-62758596 MS.gene62194:CDS 45.0%
!! AATGCAGAGGCATCATATGG+TGG + chr3.2:62758368-62758387 None:intergenic 45.0%
AACCAAGAGAGCAGCATGAG+AGG - chr3.2:62756584-62756603 MS.gene62194:CDS 50.0%
ACTGGTGGAACTCGTGGAAT+CGG - chr3.2:62756644-62756663 MS.gene62194:CDS 50.0%
ACTGTGTGTGTGTGTGTGTG+AGG + chr3.2:62757367-62757386 None:intergenic 50.0%
AGGCACAAGATGGTCTCTCA+AGG - chr3.2:62756604-62756623 MS.gene62194:CDS 50.0%
AGTGCGTGTAAGATTGGCGA+CGG + chr3.2:62757461-62757480 None:intergenic 50.0%
CCACAGTGTACACTTGCTCT+AGG - chr3.2:62756702-62756721 MS.gene62194:CDS 50.0%
CCTAGAGCAAGTGTACACTG+TGG + chr3.2:62756705-62756724 None:intergenic 50.0%
CTAGTTACTGGTGGAACTCG+TGG - chr3.2:62756638-62756657 MS.gene62194:CDS 50.0%
GAGAGCTTGTCCCTATGCTA+AGG - chr3.2:62757387-62757406 MS.gene62194:intron 50.0%
GGCACAAGATGGTCTCTCAA+GGG - chr3.2:62756605-62756624 MS.gene62194:CDS 50.0%
GTGGAGGAACTAGCTGAGTT+TGG - chr3.2:62756677-62756696 MS.gene62194:CDS 50.0%
AATCGGTCATGCTGTGGTGG+AGG - chr3.2:62756661-62756680 MS.gene62194:CDS 55.0%
AGGAAGCAGCTGGCAGACAA+AGG + chr3.2:62758610-62758629 None:intergenic 55.0%
CCAGCTGCTTCCTACATCAC+TGG - chr3.2:62758617-62758636 MS.gene62194:CDS 55.0%
CCAGTGATGTAGGAAGCAGC+TGG + chr3.2:62758620-62758639 None:intergenic 55.0%
CTGCTTACTCCACCTAGCGA+GGG - chr3.2:62757421-62757440 MS.gene62194:CDS 55.0%
GAAACACGATCATGGGCTCG+TGG - chr3.2:62758246-62758265 MS.gene62194:intron 55.0%
TCGTGGAATCGGTCATGCTG+TGG - chr3.2:62756655-62756674 MS.gene62194:CDS 55.0%
TGCCTCTCATGCTGCTCTCT+TGG + chr3.2:62756589-62756608 None:intergenic 55.0%
TGGAATCGGTCATGCTGTGG+TGG - chr3.2:62756658-62756677 MS.gene62194:CDS 55.0%
! GCAGCATGAGAGGCACAAGA+TGG - chr3.2:62756594-62756613 MS.gene62194:CDS 55.0%
GCTGCTTACTCCACCTAGCG+AGG - chr3.2:62757420-62757439 MS.gene62194:CDS 60.0%
GGAGCAACTGTGTTGCACCC+TGG - chr3.2:62758046-62758065 MS.gene62194:intron 60.0%
TCATGGGCTCGTGGCTCATC+TGG - chr3.2:62758255-62758274 MS.gene62194:intron 60.0%
! TAGCGAGGGCCTTGTGCACT+GGG - chr3.2:62757435-62757454 MS.gene62194:CDS 60.0%
CAGTGCACAAGGCCCTCGCT+AGG + chr3.2:62757436-62757455 None:intergenic 65.0%
TGCACAAGGCCCTCGCTAGG+TGG + chr3.2:62757433-62757452 None:intergenic 65.0%
! CTAGCGAGGGCCTTGTGCAC+TGG - chr3.2:62757434-62757453 MS.gene62194:CDS 65.0%
TGGCGACGGCCCAGTGCACA+AGG + chr3.2:62757447-62757466 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 62756578 62758700 62756578 ID=MS.gene62194
chr3.2 mRNA 62756578 62758700 62756578 ID=MS.gene62194.t1;Parent=MS.gene62194
chr3.2 exon 62758404 62758700 62758404 ID=MS.gene62194.t1.exon1;Parent=MS.gene62194.t1
chr3.2 CDS 62758404 62758700 62758404 ID=cds.MS.gene62194.t1;Parent=MS.gene62194.t1
chr3.2 exon 62757388 62757604 62757388 ID=MS.gene62194.t1.exon2;Parent=MS.gene62194.t1
chr3.2 CDS 62757388 62757604 62757388 ID=cds.MS.gene62194.t1;Parent=MS.gene62194.t1
chr3.2 exon 62757183 62757289 62757183 ID=MS.gene62194.t1.exon3;Parent=MS.gene62194.t1
chr3.2 CDS 62757183 62757289 62757183 ID=cds.MS.gene62194.t1;Parent=MS.gene62194.t1
chr3.2 exon 62756578 62756766 62756578 ID=MS.gene62194.t1.exon4;Parent=MS.gene62194.t1
chr3.2 CDS 62756578 62756766 62756578 ID=cds.MS.gene62194.t1;Parent=MS.gene62194.t1
Gene Sequence

>MS.gene62194

ATGGAGAACCAAGAGAGCAGCATGAGAGGCACAAGATGGTCTCTCAAGGGAAAGTCTGCTCTAGTTACTGGTGGAACTCGTGGAATCGGTCATGCTGTGGTGGAGGAACTAGCTGAGTTTGGTGCCACAGTGTACACTTGCTCTAGGAATGAAGAAGAGCTGAGCAAATGCTTAAATGAGTGGAAAGAGAAGGGTTTTTTTGTTTATGGATCGGTTTGTGATGCATCTTCTCCTTCTCAAAGAGAGGAGCTTATTCGACAAGTTGCTTCTGCTTTCAACGGCAAGCTCAACATACTTGTGAGTTTTTCCTTTTCTATATATTTCGTTTTCCGTTTCTATATTATGTTGATGTTGATGTTGAATTTTGCATAGCTGGTAGTCTTTTAATTCATGAAAATTACTCGTACAATACAAATATTCAGTTTTTAACTTCTTTTAATCAATTTGTAGATGCTCGTGGAAAACTTAGTTGGAAAAGTTTTTGAAAAATTGAAATGCCAAATTATTTTCAACTAGCACAACAGAATATATTATTTACAATTTGATTTGTATGTTTGTCTTAAGGTTATATTAATGTTTACTTTCTATAAGAATTTGTATTGTATTTATCAAACTTACTCTTATAGTCTCGTTCATCAGTTCACTTTTTGAGTGTGTTGGAGATTGATCTAATGCATTATAACTCTAACGGTGTTTCATCGTTCTGTGTATTGCTGCAACATGTTATAAGATTTTCTTCCTGGTGAAAAAATAAAATAGTAGAAGTTATAGAAGAAGGTTGAAATGCCTCACACACACACACACACAGTGAGAGCTTGTCCCTATGCTAAGGTTGTTATAAGGCTGCTTACTCCACCTAGCGAGGGCCTTGTGCACTGGGCCGTCGCCAATCTTACACGCACTTGATTTTCAAGTGGTTACATTACATACTTAAAAAAATTGCATTCTTTTGATGTACTTTGGCTTGTTATAAGACTTTCTTATGGATGCTTCAAATACACTCAGAAATGTTCACCTTTTTCGTCTTGTTTCTGGTATGCATGACTTTAACTTATAATAAAAGTTATGTTCATATGATAATCTTGTGAGCTATTAGGTAAACAATGCTGGTACAAATGTGAGGAAGCCAACAATTGAGTATACAGCCGAAGATTATTCAAAAGTGATGACTACTAACTTGGATTCTGCATTCCATCTATGTCAACTGGCACATCCTCTTCTTAAAGAATCTGGAAATGGAAGCATTGTGTTCATTTCCTCTGTTGCATCTCTGATAAGCGTAGGTACCGGAAGCATCTATGCAGTGAGTAAAGGTAACTTGGTGATCATCATGTCTACTTAATTTGCATAATGAGTACAAACTATTTTGATAGACATTTACTGAAGCGATTCACTGTGTTGTTTTCTTCAGCTGCAATCAATCAGCTGACAAAAAGTCTTGCTTGTGAATGGGCAAAAGACAATATAAGGAGCAACTGTGTTGCACCCTGGTATACAAAAACGTCGCTTGTGGAACAGGTAATGCTCTTTTCACACCTTTAATTATCCATTCTCTGGCCTTGTCATTTTTTCTTTATGAAGATTGCTTAAGTTTGTAAATATATATTTTCTCCTACGTTGCGATTCAATAATACTCTTATGATGTTACTCTGATTCACGTATGCATACGAAACACGATCATGGGCTCGTGGCTCATCTGGATGTTAGCCTGATTCATGCAACCGAAATCATGTATTAGTAGGCTAACTCTTTAAGTGTTGATCAATGAAAAACATATCTGTAACTATCCACCATATGATGCCTCTGCATTTTAATTTTTGCTGAAAAATAAACATATGTATAACCGTAGTGTCAGTGTGAGCATCTGTAGTATTAATCCAAATGGCAATATTATGTTCAGAACTTATGTGGCTAATAATTTACCTTTCTTGCAGTTTATTGCTAACAAAAAGTTTGTGAATGAAGTATTATCTCGAACACCGATAAAGCGTATAGCAGAAACACATGAAGTGTCTGCCTTGGTGACTTTCCTTTGTCTGCCAGCTGCTTCCTACATCACTGGACAAACTGTTTCTGTTGATGGAGGATTTACTGTGAATGGATTTTCTGGGTTGTTTAAATAA
Protein sequence

>MS.gene62194.t1

MENQESSMRGTRWSLKGKSALVTGGTRGIGHAVVEELAEFGATVYTCSRNEEELSKCLNEWKEKGFFVYGSVCDASSPSQREELIRQVASAFNGKLNILVNNAGTNVRKPTIEYTAEDYSKVMTTNLDSAFHLCQLAHPLLKESGNGSIVFISSVASLISVGTGSIYAVSKAAINQLTKSLACEWAKDNIRSNCVAPWYTKTSLVEQFIANKKFVNEVLSRTPIKRIAETHEVSALVTFLCLPAASYITGQTVSVDGGFTVNGFSGLFK