AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008160.t1 MTR_3g068085 95.941 271 11 0 1 271 1 271 0.0 520
MS.gene008160.t1 MTR_3g068070 88.258 264 31 0 1 264 1 264 9.76e-176 485
MS.gene008160.t1 MTR_3g068065 85.156 256 38 0 15 270 17 272 2.85e-165 459
MS.gene008160.t1 MTR_3g068155 76.692 266 62 0 6 271 10 275 2.50e-155 434
MS.gene008160.t1 MTR_3g068175 73.626 273 70 1 1 271 1 273 2.57e-148 416
MS.gene008160.t1 MTR_3g068175 72.563 277 70 2 1 271 1 277 3.71e-146 410
MS.gene008160.t1 MTR_4g064883 61.719 256 98 0 12 267 11 266 1.66e-124 355
MS.gene008160.t1 MTR_4g064887 60.305 262 104 0 1 262 1 262 1.81e-120 345
MS.gene008160.t1 MTR_7g063320 60.311 257 98 1 9 261 9 265 1.86e-112 325
MS.gene008160.t1 MTR_7g063270 60.853 258 98 1 6 260 7 264 2.46e-110 319
MS.gene008160.t1 MTR_7g063390 59.615 260 101 2 6 261 7 266 6.89e-110 318
MS.gene008160.t1 MTR_7g063360 60.385 260 99 2 6 261 7 266 9.70e-109 315
MS.gene008160.t1 MTR_7g063330 57.795 263 108 1 1 260 1 263 3.55e-108 314
MS.gene008160.t1 MTR_7g063260 58.687 259 103 1 6 260 7 265 1.07e-105 308
MS.gene008160.t1 MTR_4g064887 60.766 209 82 0 1 209 1 209 9.35e-94 276
MS.gene008160.t1 MTR_4g064883 62.626 198 74 0 12 209 11 208 8.13e-93 273
MS.gene008160.t1 MTR_7g063270 50.775 258 75 3 6 260 7 215 6.60e-81 243
MS.gene008160.t1 MTR_4g132850 32.114 246 162 4 15 259 7 248 8.02e-32 118
MS.gene008160.t1 MTR_3g085740 30.081 246 161 5 21 261 81 320 1.60e-30 116
MS.gene008160.t1 MTR_7g094510 32.283 254 148 10 20 259 11 254 2.08e-28 109
MS.gene008160.t1 MTR_7g094520 33.068 251 150 9 20 259 16 259 2.89e-28 109
MS.gene008160.t1 MTR_7g071680 31.915 282 154 8 8 260 22 294 9.39e-28 108
MS.gene008160.t1 MTR_4g058810 30.651 261 168 9 14 264 10 267 2.35e-27 107
MS.gene008160.t1 MTR_4g058840 29.730 259 171 7 15 264 11 267 2.00e-26 104
MS.gene008160.t1 MTR_4g058820 29.730 259 171 7 15 264 11 267 2.00e-26 104
MS.gene008160.t1 MTR_1196s0010 32.510 243 153 6 20 259 279 513 1.06e-24 103
MS.gene008160.t1 MTR_7g083870 30.216 278 172 8 1 260 14 287 2.78e-23 96.7
MS.gene008160.t1 MTR_4g131740 30.739 257 166 7 15 262 40 293 9.36e-22 92.4
MS.gene008160.t1 MTR_4g052350 30.651 261 160 11 15 264 14 264 1.42e-21 91.7
MS.gene008160.t1 MTR_7g107670 28.016 257 159 7 20 260 38 284 3.79e-20 88.2
MS.gene008160.t1 MTR_4g052320 28.235 255 175 6 15 264 8 259 4.49e-20 87.4
MS.gene008160.t1 MTR_3g020670 31.636 275 161 12 3 260 6 270 8.37e-20 87.0
MS.gene008160.t1 MTR_6g023990 29.104 268 171 9 4 259 2 262 1.26e-19 86.3
MS.gene008160.t1 MTR_6g023590 27.715 267 175 8 4 259 2 261 1.79e-19 85.5
MS.gene008160.t1 MTR_7g113650 31.641 256 164 8 15 262 40 292 2.32e-19 85.9
MS.gene008160.t1 MTR_3g068075 82.000 50 8 1 123 171 1 50 3.95e-19 80.1
MS.gene008160.t1 MTR_8g442750 28.125 256 169 6 15 259 14 265 5.76e-19 84.7
MS.gene008160.t1 MTR_1g051165 26.172 256 175 8 21 263 20 274 7.65e-19 84.3
MS.gene008160.t1 MTR_4g058830 34.146 164 101 6 106 264 52 213 8.36e-19 82.8
MS.gene008160.t1 MTR_4g052340 28.125 256 173 7 15 264 14 264 9.51e-19 84.0
MS.gene008160.t1 MTR_4g078630 29.670 273 168 10 3 260 8 271 1.90e-18 83.2
MS.gene008160.t1 MTR_4g052300 28.405 257 171 9 15 264 8 258 3.36e-18 82.0
MS.gene008160.t1 MTR_1g068955 30.859 256 158 9 20 263 19 267 9.54e-18 81.3
MS.gene008160.t1 MTR_1g082760 27.907 258 173 7 15 263 40 293 1.22e-17 81.3
MS.gene008160.t1 MTR_6g023600 28.090 267 174 8 4 259 2 261 3.52e-17 79.3
MS.gene008160.t1 MTR_7g105590 31.551 187 119 6 15 197 8 189 3.99e-17 77.8
MS.gene008160.t1 MTR_5g098650 68.627 51 16 0 221 271 124 174 1.98e-16 75.5
MS.gene008160.t1 MTR_6g023950 27.170 265 179 8 4 259 2 261 2.51e-16 77.0
MS.gene008160.t1 MTR_7g105460 27.500 280 177 11 4 270 2 268 2.61e-16 77.0
MS.gene008160.t1 MTR_7g105580 27.451 255 151 8 15 260 8 237 4.11e-16 76.3
MS.gene008160.t1 MTR_6g023610 27.636 275 177 10 4 265 2 267 4.54e-16 76.3
MS.gene008160.t1 MTR_2g090165 27.068 266 183 7 11 269 2 263 1.80e-15 74.7
MS.gene008160.t1 MTR_8g044240 27.632 228 149 7 15 233 69 289 5.69e-15 73.9
MS.gene008160.t1 MTR_8g089575 30.348 201 131 5 20 213 50 248 5.98e-15 73.9
MS.gene008160.t1 MTR_7g110140 27.481 262 182 6 11 267 2 260 6.13e-15 73.2
MS.gene008160.t1 MTR_6g023600 27.715 267 169 8 4 259 2 255 1.12e-14 72.4
MS.gene008160.t1 MTR_7g110130 26.641 259 177 7 11 261 2 255 1.48e-14 72.0
MS.gene008160.t1 MTR_1579s0020 35.227 88 56 1 181 268 1 87 4.96e-14 66.6
MS.gene008160.t1 MTR_4g134660 31.138 167 112 2 21 184 12 178 5.73e-14 70.1
MS.gene008160.t1 MTR_7g110160 28.016 257 157 7 11 261 2 236 9.69e-14 69.3
MS.gene008160.t1 MTR_6g041980 34.247 146 84 5 15 154 64 203 3.55e-12 65.5
MS.gene008160.t1 MTR_6g035395 27.059 255 165 7 12 261 2 240 3.75e-12 65.1
MS.gene008160.t1 MTR_3g098470 34.459 148 94 3 8 154 25 170 3.80e-12 65.9
MS.gene008160.t1 MTR_6g041980 30.144 209 122 6 15 205 64 266 6.69e-12 65.1
MS.gene008160.t1 MTR_3g113870 26.667 180 126 4 28 205 34 209 1.39e-11 63.2
MS.gene008160.t1 MTR_1275s0010 23.600 250 150 5 10 250 153 370 1.71e-11 64.3
MS.gene008160.t1 MTR_7g105570 28.191 188 116 7 94 270 46 225 1.82e-11 62.8
MS.gene008160.t1 MTR_7g080410 26.519 181 127 4 22 197 45 224 2.00e-11 63.5
MS.gene008160.t1 MTR_7g080410 26.519 181 127 4 22 197 45 224 2.76e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008160.t1 AT5G06060 69.380 258 79 0 12 269 6 263 4.68e-138 389
MS.gene008160.t1 AT2G29290 60.311 257 100 1 12 266 4 260 2.00e-118 340
MS.gene008160.t1 AT2G29260 61.176 255 99 0 12 266 65 319 2.85e-117 339
MS.gene008160.t1 AT2G29150 60.000 265 104 2 1 264 1 264 4.88e-115 331
MS.gene008160.t1 AT2G29360 59.004 261 107 0 4 264 5 265 1.11e-114 330
MS.gene008160.t1 AT1G07440 59.701 268 102 3 1 266 1 264 1.25e-114 330
MS.gene008160.t1 AT2G29350 59.091 264 107 1 1 264 1 263 7.95e-114 328
MS.gene008160.t1 AT1G07450 59.289 253 102 1 12 264 5 256 3.90e-111 321
MS.gene008160.t1 AT2G29330 60.465 258 98 3 12 266 4 260 2.98e-110 319
MS.gene008160.t1 AT2G29340 57.143 259 109 1 12 268 4 262 3.31e-109 316
MS.gene008160.t1 AT2G29340 57.588 257 107 1 12 266 4 260 1.07e-108 317
MS.gene008160.t1 AT2G30670 58.498 253 105 0 12 264 4 256 3.67e-108 314
MS.gene008160.t1 AT2G29370 54.789 261 118 0 4 264 5 265 2.42e-107 312
MS.gene008160.t1 AT2G29320 58.366 257 104 2 13 266 11 267 2.52e-105 307
MS.gene008160.t1 AT2G29310 56.917 253 109 0 12 264 4 256 4.82e-105 306
MS.gene008160.t1 AT2G29290 58.403 238 97 1 31 266 6 243 1.29e-104 304
MS.gene008160.t1 AT2G29300 56.589 258 109 2 12 266 4 261 5.97e-103 300
MS.gene008160.t1 AT2G29300 56.400 250 108 1 12 260 4 253 3.28e-99 292
MS.gene008160.t1 AT2G29350 59.111 225 91 1 40 264 2 225 1.13e-96 283
MS.gene008160.t1 AT2G29320 57.741 239 98 2 31 266 11 249 3.22e-95 280
MS.gene008160.t1 AT2G29310 55.983 234 103 0 31 264 21 254 2.02e-93 276
MS.gene008160.t1 AT2G29350 60.294 204 81 0 1 204 1 204 1.03e-88 263
MS.gene008160.t1 AT2G29340 56.061 198 87 0 12 209 4 201 4.16e-80 240
MS.gene008160.t1 AT2G29340 56.061 198 87 0 12 209 4 201 4.16e-80 240
MS.gene008160.t1 AT2G29310 56.061 198 87 0 12 209 4 201 4.19e-78 235
MS.gene008160.t1 AT2G29320 58.639 191 78 1 13 202 11 201 2.83e-77 233
MS.gene008160.t1 AT1G07440 58.140 172 69 1 1 172 1 169 1.14e-67 207
MS.gene008160.t1 AT2G29310 54.749 179 81 0 31 209 21 199 2.91e-66 205
MS.gene008160.t1 AT2G29170 57.843 102 42 1 1 101 1 102 1.09e-34 121
MS.gene008160.t1 AT4G05530 32.114 246 162 5 15 259 9 250 6.69e-32 118
MS.gene008160.t1 AT5G18210 32.950 261 160 7 10 259 3 259 1.48e-30 115
MS.gene008160.t1 AT3G03980 31.227 269 166 8 6 259 5 269 1.59e-29 112
MS.gene008160.t1 AT1G24360 30.364 247 159 7 21 261 80 319 3.91e-29 112
MS.gene008160.t1 AT4G13180 32.432 259 165 8 10 261 8 263 1.65e-28 110
MS.gene008160.t1 AT5G18210 32.234 273 156 9 10 271 3 257 1.04e-26 105
MS.gene008160.t1 AT3G46170 29.457 258 171 7 15 263 26 281 2.95e-25 101
MS.gene008160.t1 AT3G55310 30.769 260 165 11 15 263 17 272 1.10e-24 100
MS.gene008160.t1 AT1G54870 33.463 257 158 8 15 262 84 336 1.17e-24 101
MS.gene008160.t1 AT1G54870 33.463 257 158 8 15 262 82 334 1.24e-24 100
MS.gene008160.t1 AT3G55290 29.344 259 170 9 15 263 18 273 3.96e-24 98.6
MS.gene008160.t1 AT3G55290 30.000 260 167 11 15 263 17 272 4.27e-24 98.6
MS.gene008160.t1 AT1G63380 28.571 259 173 8 15 263 15 271 4.92e-24 98.6
MS.gene008160.t1 AT1G63380 28.571 259 173 8 15 263 19 275 4.96e-24 98.6
MS.gene008160.t1 AT1G63380 28.571 259 173 8 15 263 17 273 5.53e-24 98.2
MS.gene008160.t1 AT1G62610 28.185 259 174 8 15 263 13 269 6.91e-24 97.8
MS.gene008160.t1 AT1G63380 28.571 259 173 8 15 263 22 278 7.04e-24 98.2
MS.gene008160.t1 AT1G62610 28.185 259 174 8 15 263 15 271 7.16e-24 97.8
MS.gene008160.t1 AT1G62610 28.185 259 174 8 15 263 14 270 7.64e-24 97.8
MS.gene008160.t1 AT1G62610 28.185 259 174 8 15 263 19 275 9.25e-24 97.8
MS.gene008160.t1 AT3G05260 33.208 265 165 7 6 262 28 288 2.03e-23 97.1
MS.gene008160.t1 AT2G17845 28.897 263 171 9 15 264 47 306 4.49e-23 96.3
MS.gene008160.t1 AT3G04000 31.734 271 165 8 4 258 3 269 1.10e-22 94.7
MS.gene008160.t1 AT3G26760 29.699 266 169 7 8 260 30 290 3.89e-22 93.6
MS.gene008160.t1 AT3G51680 33.166 199 123 5 15 205 32 228 2.30e-20 89.0
MS.gene008160.t1 AT1G52340 29.658 263 163 8 15 260 18 275 2.56e-19 85.5
MS.gene008160.t1 AT2G47120 28.195 266 171 12 11 267 2 256 4.18e-19 84.7
MS.gene008160.t1 AT3G12800 26.070 257 174 7 15 259 10 262 4.52e-19 85.1
MS.gene008160.t1 AT2G47120 28.244 262 168 12 15 267 7 257 6.05e-19 84.3
MS.gene008160.t1 AT4G03140 26.562 256 174 6 15 260 78 329 1.55e-18 84.3
MS.gene008160.t1 AT2G47130 28.682 258 171 8 11 261 2 253 3.97e-17 79.3
MS.gene008160.t1 AT5G04900 29.778 225 141 8 18 233 80 296 1.03e-15 76.3
MS.gene008160.t1 AT3G42960 29.183 257 158 9 20 260 13 261 1.08e-14 72.8
MS.gene008160.t1 AT1G10310 33.939 165 106 3 21 184 21 183 1.24e-14 72.0
MS.gene008160.t1 AT2G47140 27.734 256 169 8 15 261 6 254 4.89e-14 70.5
MS.gene008160.t1 AT4G13250 25.581 258 153 8 19 243 163 414 1.21e-13 70.9
MS.gene008160.t1 AT1G24470 26.961 204 132 6 20 213 55 251 1.57e-13 69.7
MS.gene008160.t1 AT3G26770 25.940 266 175 7 15 264 41 300 3.63e-13 68.6
MS.gene008160.t1 AT5G10050 26.291 213 143 4 21 232 11 210 6.38e-13 67.8
MS.gene008160.t1 AT5G65205 30.556 180 114 6 21 197 12 183 6.54e-13 67.8
MS.gene008160.t1 AT3G06060 25.743 202 143 2 9 207 31 228 5.50e-12 65.5
MS.gene008160.t1 AT4G09750 30.144 209 122 6 15 205 41 243 6.20e-12 65.1
MS.gene008160.t1 AT3G06060 25.743 202 143 2 9 207 48 245 6.62e-12 65.1
MS.gene008160.t1 AT3G47350 26.540 211 140 7 20 221 49 253 1.79e-11 63.9
MS.gene008160.t1 AT3G03330 26.904 197 139 3 6 197 32 228 2.22e-11 63.5
MS.gene008160.t1 AT3G47350 26.540 211 140 7 20 221 49 253 2.35e-11 63.5

Find 50 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTCTGCTTAAAGAATC+TGG 0.325649 3.4:-69099978 MS.gene008160:CDS
AATGGGCGAAAGACAATATA+AGG 0.356013 3.4:-69099750 MS.gene008160:CDS
GGACAGATTGTTTCTGTTGA+TGG 0.357615 3.4:-69098136 MS.gene008160:CDS
GGTTCTGCTATACGCTTTAT+CGG 0.380929 3.4:+69098217 None:intergenic
GTGGAAGAACTCGCTGAGTT+TGG 0.400596 3.4:-69100481 MS.gene008160:CDS
CTGCTTAAAGAATCTGGAAA+TGG 0.406314 3.4:-69099972 MS.gene008160:CDS
GAAGCCAACAATTGAGTATA+CGG 0.419549 3.4:-69100066 MS.gene008160:CDS
CGAGAGCGTTGAATCCTTTG+AGG 0.442296 3.4:+69100539 None:intergenic
ACCGGCGGAACGCGTGGAAT+CGG 0.443421 3.4:-69100514 MS.gene008160:CDS
GGATTAGGTAATCCTCATGC+TGG 0.447027 3.4:+69098087 None:intergenic
GATGGAGGATTTACTGTGAA+TGG 0.451398 3.4:-69098118 MS.gene008160:CDS
TCAGCCGTATACTCAATTGT+TGG 0.456707 3.4:+69100062 None:intergenic
TCTCTCACAAGCATAGGTTC+TGG 0.458121 3.4:-69099921 MS.gene008160:CDS
TGCATACCATCTATGTCAAT+TGG 0.460251 3.4:-69100003 MS.gene008160:CDS
AGCGTTTATTCAGCTCCTCT+TGG 0.468728 3.4:+69100431 None:intergenic
GAAAGTGATGACTACTAACT+TGG 0.480086 3.4:-69100030 MS.gene008160:CDS
CTCGTCACCGGCGGAACGCG+TGG 0.489333 3.4:-69100520 MS.gene008160:CDS
TCCGATTCCACGCGTTCCGC+CGG 0.497124 3.4:+69100513 None:intergenic
TGAGTTTAGGTGAACAATGC+TGG 0.497981 3.4:-69100101 MS.gene008160:intron
AATGTTGCTTCTTCTTTCAA+CGG 0.501278 3.4:-69100322 MS.gene008160:CDS
CCAGCTGCTTCCTACATCAC+TGG 0.520639 3.4:-69098157 MS.gene008160:CDS
GGATTCAACGCTCTCGTCAC+CGG 0.529505 3.4:-69100532 MS.gene008160:CDS
ACCACATGAAGTGTCTTCCT+TGG 0.531653 3.4:-69098197 MS.gene008160:CDS
TGATGTAGGAAGCAGCTGGC+AGG 0.543439 3.4:+69098161 None:intergenic
TTAAACGAGTGGAAAGAGAA+AGG 0.546612 3.4:-69100409 MS.gene008160:CDS
AGCGTCTATGCAGCAAGTAA+AGG 0.551642 3.4:-69099897 MS.gene008160:intron
AACAATCTGTCCAGTGATGT+AGG 0.551671 3.4:+69098147 None:intergenic
GATGTACCAATTGACATAGA+TGG 0.556385 3.4:+69099997 None:intergenic
ATCTTCTTCTTCTCAAAGAG+AGG 0.556668 3.4:-69100356 MS.gene008160:CDS
TTTCCAGATTCTTTAAGCAG+AGG 0.567292 3.4:+69099975 None:intergenic
GATTAGGTAATCCTCATGCT+GGG 0.570838 3.4:+69098088 None:intergenic
CAGATTGTTTCTGTTGATGG+AGG 0.575569 3.4:-69098133 MS.gene008160:CDS
GAGAGCGTTGAATCCTTTGA+GGG 0.575644 3.4:+69100540 None:intergenic
AGCTCAAGATGGTCCCTCAA+AGG 0.580641 3.4:-69100553 MS.gene008160:CDS
AGGAAGCAGCTGGCAGGCAA+AGG 0.580855 3.4:+69098167 None:intergenic
CACTTGTTCTAGAAACCAAG+AGG 0.591688 3.4:-69100446 MS.gene008160:CDS
CCAGTGATGTAGGAAGCAGC+TGG 0.595402 3.4:+69098157 None:intergenic
AGCATTACATGTTCCACAAG+TGG 0.604747 3.4:+69099694 None:intergenic
GTTGCATCTCTCACAAGCAT+AGG 0.610660 3.4:-69099927 MS.gene008160:CDS
GCAGTAGCAGAAGCTCAAGA+TGG 0.612756 3.4:-69100564 MS.gene008160:CDS
ATGGATTTCAACCCAGCATG+AGG 0.617391 3.4:-69098099 MS.gene008160:CDS
TGAATAAACGCTTAAACGAG+TGG 0.622975 3.4:-69100420 MS.gene008160:CDS
GGAGCAACTGCGTTGCACCC+TGG 0.632107 3.4:-69099729 MS.gene008160:CDS
TGGAATCGGACATGCTATCG+TGG 0.642183 3.4:-69100500 MS.gene008160:CDS
TTCAACGCTCTCGTCACCGG+CGG 0.643369 3.4:-69100529 MS.gene008160:CDS
GTATACGAAAACACCACTTG+TGG 0.645433 3.4:-69099707 MS.gene008160:CDS
ACCAAGGAAGACACTTCATG+TGG 0.707139 3.4:+69098196 None:intergenic
CAGGCAAAGGAAAGTCACCA+AGG 0.712267 3.4:+69098180 None:intergenic
TCTAGAACAAGTGTAAACTG+TGG 0.727754 3.4:+69100456 None:intergenic
ACAATGCTGGAACAAATGTG+AGG 0.761871 3.4:-69100088 MS.gene008160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAGTATTAATCTATAAA+TGG - chr3.4:69100337-69100356 MS.gene008160:CDS 15.0%
!! CAAAAGAAAATATATAGAAA+AGG + chr3.4:69098400-69098419 None:intergenic 15.0%
!!! TATTCTTCTATTTTGTTATT+AGG - chr3.4:69099128-69099147 MS.gene008160:intron 15.0%
!! AATTCTTTAGATTATTGAAC+AGG - chr3.4:69099271-69099290 MS.gene008160:intron 20.0%
!! ATCCATTATATTTAAGCAAA+AGG + chr3.4:69098829-69098848 None:intergenic 20.0%
!! TAGAACCAAAAAGAATAAAT+TGG - chr3.4:69099539-69099558 MS.gene008160:intron 20.0%
!! TTTGTAATCATGATTTGATT+TGG - chr3.4:69099061-69099080 MS.gene008160:intron 20.0%
!!! AACCTTTTGCTTAAATATAA+TGG - chr3.4:69098824-69098843 MS.gene008160:intron 20.0%
!!! AGTAACCAATTTATTCTTTT+TGG + chr3.4:69099547-69099566 None:intergenic 20.0%
!!! TTTTGCTTAAATATAATGGA+TGG - chr3.4:69098828-69098847 MS.gene008160:intron 20.0%
! AAAAAATCTTGCTTGTGAAT+GGG - chr3.4:69098903-69098922 MS.gene008160:intron 25.0%
! ATCAAAATTGCTTGCATATT+TGG - chr3.4:69099297-69099316 MS.gene008160:intron 25.0%
! ATGTATATGATACACAATTG+TGG - chr3.4:69099875-69099894 MS.gene008160:intron 25.0%
! ATTCATTTAGTATTGCATAC+AGG - chr3.4:69099773-69099792 MS.gene008160:CDS 25.0%
! ATTGAAGATATATCCAATGT+CGG + chr3.4:69098493-69098512 None:intergenic 25.0%
! ATTGGTCATTGTTTAATCAA+GGG - chr3.4:69099098-69099117 MS.gene008160:intron 25.0%
! ATTGTTTAATCAAGGGAATA+TGG - chr3.4:69099105-69099124 MS.gene008160:intron 25.0%
! CAATGTAATTACTTCCATAA+AGG - chr3.4:69099437-69099456 MS.gene008160:intron 25.0%
! CATACGAATAAGAAATGAAT+TGG - chr3.4:69100081-69100100 MS.gene008160:CDS 25.0%
! TAAAAAAAATGACAAAGCCA+TGG + chr3.4:69099026-69099045 None:intergenic 25.0%
! TACTCTTATCACAAGTTTAA+TGG - chr3.4:69099366-69099385 MS.gene008160:intron 25.0%
! TCAAAATTGCTTGCATATTT+GGG - chr3.4:69099298-69099317 MS.gene008160:intron 25.0%
! TGATTATCAAATGACCTTTA+TGG + chr3.4:69099454-69099473 None:intergenic 25.0%
! TTCATTTAGTATTGCATACA+GGG - chr3.4:69099774-69099793 MS.gene008160:CDS 25.0%
!! GACATACTAAAACATGATTT+TGG + chr3.4:69099935-69099954 None:intergenic 25.0%
!!! ACATTCATACTCATTTTTGT+AGG - chr3.4:69099190-69099209 MS.gene008160:intron 25.0%
AATTCACAATATTTGCACCT+AGG - chr3.4:69099498-69099517 MS.gene008160:intron 30.0%
ACCTTCAATTATTCATTCCA+TGG - chr3.4:69099006-69099025 MS.gene008160:intron 30.0%
AGTCCTCAACTATAAAAGAT+TGG + chr3.4:69099957-69099976 None:intergenic 30.0%
ATAGTTGAGGACTGTATATT+TGG - chr3.4:69099964-69099983 MS.gene008160:CDS 30.0%
ATTAATACTACATGAGCACA+CGG + chr3.4:69100330-69100349 None:intergenic 30.0%
ATTCACAATATTTGCACCTA+GGG - chr3.4:69099499-69099518 MS.gene008160:intron 30.0%
CAAAAAATCTTGCTTGTGAA+TGG - chr3.4:69098902-69098921 MS.gene008160:intron 30.0%
TATGATGTTCTTGTGAGTTT+AGG - chr3.4:69098556-69098575 MS.gene008160:intron 30.0%
TTAAACAATGACCAATCAGT+CGG + chr3.4:69099094-69099113 None:intergenic 30.0%
! AATGTTGCTTCTTCTTTCAA+CGG - chr3.4:69098348-69098367 MS.gene008160:intron 30.0%
! ATATCTTCAATCAGAAGTGT+CGG - chr3.4:69098502-69098521 MS.gene008160:intron 30.0%
! GATTGGTCATTGTTTAATCA+AGG - chr3.4:69099097-69099116 MS.gene008160:intron 30.0%
! TGTCCAATCTTTTATAGTTG+AGG - chr3.4:69099951-69099970 MS.gene008160:CDS 30.0%
! TTAATGGTTTTCCATGTTGA+CGG - chr3.4:69099382-69099401 MS.gene008160:intron 30.0%
!! AAGAAATGAATTGGCTTTGA+GGG - chr3.4:69100090-69100109 MS.gene008160:CDS 30.0%
!! TAAGAAATGAATTGGCTTTG+AGG - chr3.4:69100089-69100108 MS.gene008160:CDS 30.0%
!!! ACTTTTTTGTTAAGACGCAT+GGG - chr3.4:69100133-69100152 MS.gene008160:intron 30.0%
!!! TTTTGGTTCTAACTGACAAA+TGG + chr3.4:69099530-69099549 None:intergenic 30.0%
AATGGGCGAAAGACAATATA+AGG - chr3.4:69098920-69098939 MS.gene008160:intron 35.0%
AATTGCTTGCATATTTGGGA+TGG - chr3.4:69099302-69099321 MS.gene008160:intron 35.0%
ACATACGTGAATCAAACAGA+AGG + chr3.4:69099859-69099878 None:intergenic 35.0%
ATCCCTTCACAAATTGTCTA+TGG - chr3.4:69099673-69099692 MS.gene008160:intron 35.0%
ATCTTCTTCTTCTCAAAGAG+AGG - chr3.4:69098314-69098333 MS.gene008160:intron 35.0%
CTGCTTAAAGAATCTGGAAA+TGG - chr3.4:69098698-69098717 MS.gene008160:intron 35.0%
GAAAGTGATGACTACTAACT+TGG - chr3.4:69098640-69098659 MS.gene008160:intron 35.0%
GAAGCCAACAATTGAGTATA+CGG - chr3.4:69098604-69098623 MS.gene008160:intron 35.0%
GAGGACTGTATATTTGGATA+TGG - chr3.4:69099970-69099989 MS.gene008160:CDS 35.0%
GATGTACCAATTGACATAGA+TGG + chr3.4:69098676-69098695 None:intergenic 35.0%
GCCATGGAATGAATAATTGA+AGG + chr3.4:69099010-69099029 None:intergenic 35.0%
TAATCGTCAGTGTTGTCAAA+TGG + chr3.4:69099739-69099758 None:intergenic 35.0%
TGAATAAACGCTTAAACGAG+TGG - chr3.4:69098250-69098269 MS.gene008160:CDS 35.0%
TGCATACCATCTATGTCAAT+TGG - chr3.4:69098667-69098686 MS.gene008160:intron 35.0%
TTAAACGAGTGGAAAGAGAA+AGG - chr3.4:69098261-69098280 MS.gene008160:CDS 35.0%
TTGGTTTATCTGTGTATGGA+GGG - chr3.4:69100239-69100258 MS.gene008160:intron 35.0%
TTTCCAGATTCTTTAAGCAG+AGG + chr3.4:69098698-69098717 None:intergenic 35.0%
! GAGAAAGGTTTTTCTGTTCA+TGG - chr3.4:69098276-69098295 MS.gene008160:intron 35.0%
! GATACATTTTTCCGTCAACA+TGG + chr3.4:69099396-69099415 None:intergenic 35.0%
! TCTAGAACAAGTGTAAACTG+TGG + chr3.4:69098217-69098236 None:intergenic 35.0%
! TGGCTCTTTAACATTTTTCC+TGG - chr3.4:69100158-69100177 MS.gene008160:intron 35.0%
!! ATGATTTTGGCATCTTCACA+TGG + chr3.4:69099922-69099941 None:intergenic 35.0%
!!! GACTTTTTTGTTAAGACGCA+TGG - chr3.4:69100132-69100151 MS.gene008160:intron 35.0%
AACAATCTGTCCAGTGATGT+AGG + chr3.4:69100526-69100545 None:intergenic 40.0%
AACAGAAGGCAGTGGAATTA+TGG + chr3.4:69099845-69099864 None:intergenic 40.0%
ACAATGCTGGAACAAATGTG+AGG - chr3.4:69098582-69098601 MS.gene008160:intron 40.0%
ACAGTTGTAGCAAGTGATTC+TGG - chr3.4:69100025-69100044 MS.gene008160:CDS 40.0%
AGCATTACATGTTCCACAAG+TGG + chr3.4:69098979-69098998 None:intergenic 40.0%
CACATGGATCAAGGTAACAT+TGG + chr3.4:69099906-69099925 None:intergenic 40.0%
CAGTTGTAGCAAGTGATTCT+GGG - chr3.4:69100026-69100045 MS.gene008160:CDS 40.0%
CATCCTCTGCTTAAAGAATC+TGG - chr3.4:69098692-69098711 MS.gene008160:intron 40.0%
CCTTCACAAATTGTCTATGG+CGG - chr3.4:69099676-69099695 MS.gene008160:intron 40.0%
CGCCATAGACAATTTGTGAA+GGG + chr3.4:69099678-69099697 None:intergenic 40.0%
GATGGAGGATTTACTGTGAA+TGG - chr3.4:69100552-69100571 MS.gene008160:CDS 40.0%
GGGCATCAATATGGCAAATA+TGG - chr3.4:69100110-69100129 MS.gene008160:intron 40.0%
GGTTCTGCTATACGCTTTAT+CGG + chr3.4:69100456-69100475 None:intergenic 40.0%
GTACTTGTCTGTAGTCTTTG+AGG - chr3.4:69099242-69099261 MS.gene008160:intron 40.0%
GTATACGAAAACACCACTTG+TGG - chr3.4:69098963-69098982 MS.gene008160:intron 40.0%
TCAGCCGTATACTCAATTGT+TGG + chr3.4:69098611-69098630 None:intergenic 40.0%
TGAGTTTAGGTGAACAATGC+TGG - chr3.4:69098569-69098588 MS.gene008160:intron 40.0%
TTTGTTAAGACGCATGGGTT+TGG - chr3.4:69100138-69100157 MS.gene008160:intron 40.0%
! AAGTGGTGTTTTCGTATACC+AGG + chr3.4:69098962-69098981 None:intergenic 40.0%
! AGTGGTGTTTTCGTATACCA+GGG + chr3.4:69098961-69098980 None:intergenic 40.0%
! GTTGGTTTATCTGTGTATGG+AGG - chr3.4:69100238-69100257 MS.gene008160:intron 40.0%
! TGGAGGGTTTTCTGCTATAT+AGG - chr3.4:69100255-69100274 MS.gene008160:intron 40.0%
!! CACTTGTTCTAGAAACCAAG+AGG - chr3.4:69098224-69098243 MS.gene008160:CDS 40.0%
!! CAGATTGTTTCTGTTGATGG+AGG - chr3.4:69100537-69100556 MS.gene008160:CDS 40.0%
!! GGACAGATTGTTTCTGTTGA+TGG - chr3.4:69100534-69100553 MS.gene008160:CDS 40.0%
!! GTGGTTGGTTTATCTGTGTA+TGG - chr3.4:69100235-69100254 MS.gene008160:intron 40.0%
ACCAAGGAAGACACTTCATG+TGG + chr3.4:69100477-69100496 None:intergenic 45.0%
AGCGTCTATGCAGCAAGTAA+AGG - chr3.4:69098773-69098792 MS.gene008160:intron 45.0%
AGCGTTTATTCAGCTCCTCT+TGG + chr3.4:69098242-69098261 None:intergenic 45.0%
ATGGATTTCAACCCAGCATG+AGG - chr3.4:69100571-69100590 MS.gene008160:CDS 45.0%
CCGCCATAGACAATTTGTGA+AGG + chr3.4:69099679-69099698 None:intergenic 45.0%
CGATGGTGCAATATGACAGA+AGG - chr3.4:69099596-69099615 MS.gene008160:intron 45.0%
GTGAATCAAACAGAAGGCAG+TGG + chr3.4:69099853-69099872 None:intergenic 45.0%
GTGATTACCAGTGTTGTCGA+TGG - chr3.4:69099579-69099598 MS.gene008160:intron 45.0%
GTTGCATCTCTCACAAGCAT+AGG - chr3.4:69098743-69098762 MS.gene008160:intron 45.0%
TATTGCACCATCGACAACAC+TGG + chr3.4:69099589-69099608 None:intergenic 45.0%
TCTCTCACAAGCATAGGTTC+TGG - chr3.4:69098749-69098768 MS.gene008160:intron 45.0%
TGGCATCTTCACATGGATCA+AGG + chr3.4:69099915-69099934 None:intergenic 45.0%
TTGTAGCAAGTGATTCTGGG+TGG - chr3.4:69100029-69100048 MS.gene008160:CDS 45.0%
! ACCACATGAAGTGTCTTCCT+TGG - chr3.4:69100473-69100492 MS.gene008160:CDS 45.0%
! GCTGCAATGGCGTATTTCTA+TGG + chr3.4:69099632-69099651 None:intergenic 45.0%
! TTGGATGAACACCGACTGAT+TGG - chr3.4:69099080-69099099 MS.gene008160:intron 45.0%
!! GAGAGCGTTGAATCCTTTGA+GGG + chr3.4:69098133-69098152 None:intergenic 45.0%
!! TGGCTTTGAGGGCATCAATA+TGG - chr3.4:69100101-69100120 MS.gene008160:intron 45.0%
ACCACCTACACAAACACAGC+AGG + chr3.4:69100220-69100239 None:intergenic 50.0%
ACTAGAGCATGTGCAGATCC+AGG + chr3.4:69100179-69100198 None:intergenic 50.0%
AGCTCAAGATGGTCCCTCAA+AGG - chr3.4:69098117-69098136 MS.gene008160:CDS 50.0%
CAGGCAAAGGAAAGTCACCA+AGG + chr3.4:69100493-69100512 None:intergenic 50.0%
CATGTGTCTGTGTCCGACAT+TGG - chr3.4:69098477-69098496 MS.gene008160:intron 50.0%
GCAGTAGCAGAAGCTCAAGA+TGG - chr3.4:69098106-69098125 MS.gene008160:CDS 50.0%
GCTGTGTTTGTGTAGGTGGT+TGG - chr3.4:69100220-69100239 MS.gene008160:intron 50.0%
GTGGAAGAACTCGCTGAGTT+TGG - chr3.4:69098189-69098208 MS.gene008160:CDS 50.0%
GTGTTGTCAAATGGCTGCCA+TGG + chr3.4:69099730-69099749 None:intergenic 50.0%
TGGAATCGGACATGCTATCG+TGG - chr3.4:69098170-69098189 MS.gene008160:CDS 50.0%
TGTGCCTGCTGTGTTTGTGT+AGG - chr3.4:69100213-69100232 MS.gene008160:intron 50.0%
!! CGAGAGCGTTGAATCCTTTG+AGG + chr3.4:69098134-69098153 None:intergenic 50.0%
ACCGACACGTCATTCTGCCA+TGG - chr3.4:69099710-69099729 MS.gene008160:CDS 55.0%
CCAGCTGCTTCCTACATCAC+TGG - chr3.4:69100513-69100532 MS.gene008160:CDS 55.0%
CCAGTGATGTAGGAAGCAGC+TGG + chr3.4:69100516-69100535 None:intergenic 55.0%
CTGACAAATGGCGCTTCCCT+AGG + chr3.4:69099518-69099537 None:intergenic 55.0%
GCCATGGCAGAATGACGTGT+CGG + chr3.4:69099714-69099733 None:intergenic 55.0%
GCCTGCTGTGTTTGTGTAGG+TGG - chr3.4:69100216-69100235 MS.gene008160:intron 55.0%
GGATTCAACGCTCTCGTCAC+CGG - chr3.4:69098138-69098157 MS.gene008160:CDS 55.0%
TGATGTAGGAAGCAGCTGGC+AGG + chr3.4:69100512-69100531 None:intergenic 55.0%
TGGCATGGCATAGGCTGCAA+TGG + chr3.4:69099645-69099664 None:intergenic 55.0%
TTGCAGCCTATGCCATGCCA+TGG - chr3.4:69099645-69099664 MS.gene008160:intron 55.0%
TTGTGAAGGGATGCCTGCCA+TGG + chr3.4:69099665-69099684 None:intergenic 55.0%
AAGGGATGCCTGCCATGGCA+TGG + chr3.4:69099660-69099679 None:intergenic 60.0%
AGCCTATGCCATGCCATGGC+AGG - chr3.4:69099649-69099668 MS.gene008160:intron 60.0%
AGGAAGCAGCTGGCAGGCAA+AGG + chr3.4:69100506-69100525 None:intergenic 60.0%
TGCCTGCCATGGCATGGCAT+AGG + chr3.4:69099654-69099673 None:intergenic 60.0%
TTCAACGCTCTCGTCACCGG+CGG - chr3.4:69098141-69098160 MS.gene008160:CDS 60.0%
ACCGGCGGAACGCGTGGAAT+CGG - chr3.4:69098156-69098175 MS.gene008160:CDS 65.0%
GGAGCAACTGCGTTGCACCC+TGG - chr3.4:69098941-69098960 MS.gene008160:intron 65.0%
TCCGATTCCACGCGTTCCGC+CGG + chr3.4:69098160-69098179 None:intergenic 65.0%
CTCGTCACCGGCGGAACGCG+TGG - chr3.4:69098150-69098169 MS.gene008160:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 69098090 69100602 69098090 ID=MS.gene008160
chr3.4 mRNA 69098090 69100602 69098090 ID=MS.gene008160.t1;Parent=MS.gene008160
chr3.4 exon 69100306 69100602 69100306 ID=MS.gene008160.t1.exon1;Parent=MS.gene008160.t1
chr3.4 CDS 69100306 69100602 69100306 ID=cds.MS.gene008160.t1;Parent=MS.gene008160.t1
chr3.4 exon 69099898 69100114 69099898 ID=MS.gene008160.t1.exon2;Parent=MS.gene008160.t1
chr3.4 CDS 69099898 69100114 69099898 ID=cds.MS.gene008160.t1;Parent=MS.gene008160.t1
chr3.4 exon 69099702 69099808 69099702 ID=MS.gene008160.t1.exon3;Parent=MS.gene008160.t1
chr3.4 CDS 69099702 69099808 69099702 ID=cds.MS.gene008160.t1;Parent=MS.gene008160.t1
chr3.4 exon 69098090 69098284 69098090 ID=MS.gene008160.t1.exon4;Parent=MS.gene008160.t1
chr3.4 CDS 69098090 69098284 69098090 ID=cds.MS.gene008160.t1;Parent=MS.gene008160.t1
Gene Sequence

>MS.gene008160

ATGGCGAACGCAGAGAGCAGTAGCAGAAGCTCAAGATGGTCCCTCAAAGGATTCAACGCTCTCGTCACCGGCGGAACGCGTGGAATCGGACATGCTATCGTGGAAGAACTCGCTGAGTTTGGTGCCACAGTTTACACTTGTTCTAGAAACCAAGAGGAGCTGAATAAACGCTTAAACGAGTGGAAAGAGAAAGGTTTTTCTGTTCATGGATCTGTTTGTGATGCATCTTCTTCTTCTCAAAGAGAGGAGCTTATTCGAAATGTTGCTTCTTCTTTCAACGGCAAACTCAACATATTTGTGAGTTTTTCCTTTTCTATATATTTTCTTTTGCATTTGTATATAATGTTGATATTGAAATTAAGAAAATGACGTAATTGTATGTAATCACATGTGTCTGTGTCCGACATTGGATATATCTTCAATCAGAAGTGTCGGTGTTATGTTATGACTTCAGCTCATATGTTGATATGATGTTCTTGTGAGTTTAGGTGAACAATGCTGGAACAAATGTGAGGAAGCCAACAATTGAGTATACGGCTGAAGATTATTCGAAAGTGATGACTACTAACTTGGATTCTGCATACCATCTATGTCAATTGGTACATCCTCTGCTTAAAGAATCTGGAAATGGAAGCATTGTGTTCATTTCATCTGTTGCATCTCTCACAAGCATAGGTTCTGGAAGCGTCTATGCAGCAAGTAAAGGTAATTCAATGTATCATTATGTTATCTTTAACCTTTTGCTTAAATATAATGGATGGAAACTAAAAAATTCATTGTGTTGTTTTCTTCAGCTGCAATCAATCAGTTGACAAAAAATCTTGCTTGTGAATGGGCGAAAGACAATATAAGGAGCAACTGCGTTGCACCCTGGTATACGAAAACACCACTTGTGGAACATGTAATGCTCTTTCACACCTTCAATTATTCATTCCATGGCTTTGTCATTTTTTTTATGAATATTGTTTGAATTTGTAATCATGATTTGATTTGGATGAACACCGACTGATTGGTCATTGTTTAATCAAGGGAATATGGTATTCTTCTATTTTGTTATTAGGTTCTTCATAGTAAATAATGATCTGTTCTTTGATGTTTTTACATTCATACTCATTTTTGTAGGATTAGTAAGAATTAAGATACAACTTAGAAGTACTTGTCTGTAGTCTTTGAGGCTCGATAATTCTTTAGATTATTGAACAGGAAAATCAAAATTGCTTGCATATTTGGGATGGCTCATATAGAGTCTTGTATAGCTGCATTGTGATTTCATTAGTACTCTTATCACAAGTTTAATGGTTTTCCATGTTGACGGAAAAATGTATCTTTTAGTGTTTTGTTTTCAATCAATGTAATTACTTCCATAAAGGTCATTTGATAATCAGACACTTTAAAGCATTAACAAACAAATTCACAATATTTGCACCTAGGGAAGCGCCATTTGTCAGTTAGAACCAAAAAGAATAAATTGGTTACTTCTTTCTTCTCAGTGATTACCAGTGTTGTCGATGGTGCAATATGACAGAAGGCTAAAATACGCCATAGAAATACGCCATTGCAGCCTATGCCATGCCATGGCAGGCATCCCTTCACAAATTGTCTATGGCGGTTGTCATAAAAACCGACACGTCATTCTGCCATGGCAGCCATTTGACAACACTGACGATTACTTGCATTACATAAATTCATTTAGTATTGCATACAGGGTGACTTAAATGACAACTTTTTAACTGATCTGACAGTTTTTCTTCTCCATAATTCCACTGCCTTCTGTTTGATTCACGTATGTATATGATACACAATTGTGGCTCATCCAATGTTACCTTGATCCATGTGAAGATGCCAAAATCATGTTTTAGTATGTCCAATCTTTTATAGTTGAGGACTGTATATTTGGATATGGAACATTGATCTTTCTATGTGTTATTGTATTACACAGTTGTAGCAAGTGATTCTGGGTGGTCGTTTAGAATAAACTTCTTAAATTAATACATACGAATAAGAAATGAATTGGCTTTGAGGGCATCAATATGGCAAATATGGACTTTTTTGTTAAGACGCATGGGTTTGGCTCTTTAACATTTTTCCTGGATCTGCACATGCTCTAGTGTAGTGTGCTGTGCTGTGCCTGCTGTGTTTGTGTAGGTGGTTGGTTTATCTGTGTATGGAGGGTTTTCTGCTATATAGGCTAACTCATTAAGTGCTGATGAATCAAAACACATCTGTGACTCTAATGTCCGTGTGCTCATGTAGTATTAATCTATAAATGGCAATGTTATGTTCATAACTTGTGTTGCGAATTGTTGTCTTTCTTACAGTTAATTGCTAACGAAGAGTTTGTGAATCAAGTGCTGTCTCGAACACCGATAAAGCGTATAGCAGAACCACATGAAGTGTCTTCCTTGGTGACTTTCCTTTGCCTGCCAGCTGCTTCCTACATCACTGGACAGATTGTTTCTGTTGATGGAGGATTTACTGTGAATGGATTTCAACCCAGCATGAGGATTACCTAA
Protein sequence

>MS.gene008160.t1

MANAESSSRSSRWSLKGFNALVTGGTRGIGHAIVEELAEFGATVYTCSRNQEELNKRLNEWKEKGFSVHGSVCDASSSSQREELIRNVASSFNGKLNIFVNNAGTNVRKPTIEYTAEDYSKVMTTNLDSAYHLCQLVHPLLKESGNGSIVFISSVASLTSIGSGSVYAASKAAINQLTKNLACEWAKDNIRSNCVAPWYTKTPLVEHLIANEEFVNQVLSRTPIKRIAEPHEVSSLVTFLCLPAASYITGQIVSVDGGFTVNGFQPSMRIT