AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008153.t1 MTR_3g068155 96.324 272 10 0 2 273 4 275 0.0 543
MS.gene008153.t1 MTR_3g068175 78.388 273 59 0 1 273 1 273 7.41e-159 442
MS.gene008153.t1 MTR_3g068175 77.256 277 59 1 1 273 1 277 1.68e-156 437
MS.gene008153.t1 MTR_3g068085 77.043 257 59 0 17 273 15 271 6.67e-151 422
MS.gene008153.t1 MTR_3g068070 75.385 260 64 0 7 266 5 264 7.06e-149 417
MS.gene008153.t1 MTR_3g068065 71.324 272 78 0 1 272 1 272 1.59e-142 401
MS.gene008153.t1 MTR_4g064883 64.591 257 91 0 13 269 10 266 2.30e-129 368
MS.gene008153.t1 MTR_4g064887 61.390 259 100 0 5 263 3 261 1.35e-120 345
MS.gene008153.t1 MTR_7g063320 57.795 263 107 1 5 263 3 265 1.17e-109 318
MS.gene008153.t1 MTR_7g063390 58.462 260 104 1 8 263 7 266 5.42e-106 308
MS.gene008153.t1 MTR_7g063270 56.977 258 108 1 8 262 7 264 8.69e-105 305
MS.gene008153.t1 MTR_7g063330 56.107 262 112 1 4 262 2 263 1.24e-104 305
MS.gene008153.t1 MTR_7g063360 57.308 260 107 1 8 263 7 266 3.38e-103 301
MS.gene008153.t1 MTR_7g063260 55.598 259 111 1 8 262 7 265 2.76e-101 296
MS.gene008153.t1 MTR_4g064883 66.162 198 67 0 13 210 10 207 9.75e-97 283
MS.gene008153.t1 MTR_4g064887 58.937 207 85 0 5 211 3 209 5.80e-90 266
MS.gene008153.t1 MTR_7g063270 48.450 258 81 4 8 262 7 215 4.45e-78 236
MS.gene008153.t1 MTR_4g132850 32.377 244 160 4 19 261 9 248 5.94e-31 116
MS.gene008153.t1 MTR_3g085740 32.114 246 156 5 23 263 81 320 3.59e-30 115
MS.gene008153.t1 MTR_7g071680 32.847 274 148 7 17 262 29 294 6.10e-29 112
MS.gene008153.t1 MTR_7g094510 30.435 253 162 7 17 260 6 253 6.96e-26 102
MS.gene008153.t1 MTR_7g094520 31.154 260 161 8 13 261 7 259 1.01e-25 102
MS.gene008153.t1 MTR_4g052350 32.000 250 163 6 17 263 14 259 4.21e-25 100
MS.gene008153.t1 MTR_1196s0010 31.818 242 154 5 22 260 279 512 1.84e-24 102
MS.gene008153.t1 MTR_4g052320 29.600 250 170 5 17 263 8 254 1.42e-22 94.4
MS.gene008153.t1 MTR_4g058810 27.203 261 177 9 16 266 10 267 1.84e-22 94.0
MS.gene008153.t1 MTR_4g052300 29.200 250 170 6 17 263 8 253 5.36e-22 92.4
MS.gene008153.t1 MTR_4g058840 28.077 260 174 9 17 266 11 267 5.39e-22 92.8
MS.gene008153.t1 MTR_4g058820 28.077 260 174 9 17 266 11 267 5.39e-22 92.8
MS.gene008153.t1 MTR_7g107670 28.731 268 173 8 8 264 26 286 1.89e-21 91.7
MS.gene008153.t1 MTR_7g083870 31.047 277 165 9 4 262 19 287 6.18e-21 90.5
MS.gene008153.t1 MTR_4g078630 29.344 259 164 8 17 262 19 271 7.54e-21 89.7
MS.gene008153.t1 MTR_4g052340 28.030 264 166 8 17 265 14 268 8.80e-21 89.4
MS.gene008153.t1 MTR_8g442750 29.545 264 155 8 17 261 14 265 2.22e-20 89.0
MS.gene008153.t1 MTR_5g098650 80.392 51 10 0 223 273 124 174 8.08e-20 84.7
MS.gene008153.t1 MTR_3g020670 31.136 273 169 11 3 262 4 270 1.68e-18 83.2
MS.gene008153.t1 MTR_4g131740 28.794 257 171 6 16 263 39 292 1.73e-18 83.6
MS.gene008153.t1 MTR_6g023990 28.736 261 174 8 9 261 6 262 2.47e-17 79.7
MS.gene008153.t1 MTR_1g068955 29.070 258 158 9 17 259 14 261 2.57e-17 80.1
MS.gene008153.t1 MTR_6g023590 29.249 253 167 8 17 261 13 261 5.99e-16 75.9
MS.gene008153.t1 MTR_7g110130 28.295 258 174 8 14 264 3 256 7.26e-16 75.9
MS.gene008153.t1 MTR_1g082760 26.357 258 177 7 17 265 40 293 8.45e-16 75.9
MS.gene008153.t1 MTR_3g068075 52.239 67 31 1 125 190 1 67 1.80e-15 70.1
MS.gene008153.t1 MTR_4g058830 29.747 158 105 5 114 266 57 213 2.47e-15 73.6
MS.gene008153.t1 MTR_7g105460 28.520 277 174 12 8 272 4 268 2.58e-15 74.3
MS.gene008153.t1 MTR_1g051165 25.670 261 182 7 17 265 14 274 3.24e-15 74.3
MS.gene008153.t1 MTR_6g023950 28.854 253 168 8 17 261 13 261 1.35e-14 72.0
MS.gene008153.t1 MTR_1579s0020 36.364 88 55 1 183 270 1 87 1.52e-14 67.8
MS.gene008153.t1 MTR_2g090165 28.794 257 173 9 14 264 3 255 1.56e-14 72.0
MS.gene008153.t1 MTR_7g105580 26.210 248 159 6 17 260 8 235 7.45e-14 70.1
MS.gene008153.t1 MTR_7g105590 27.979 193 130 6 12 201 5 191 2.14e-13 67.8
MS.gene008153.t1 MTR_6g023600 29.249 253 167 8 17 261 13 261 2.95e-13 68.6
MS.gene008153.t1 MTR_8g089575 28.505 214 142 6 19 225 47 256 6.98e-13 67.8
MS.gene008153.t1 MTR_7g110140 27.099 262 181 8 14 269 3 260 7.50e-12 64.3
MS.gene008153.t1 MTR_8g044240 27.419 186 124 5 17 194 69 251 1.71e-11 63.9
MS.gene008153.t1 MTR_3g113870 26.667 180 126 4 30 207 34 209 6.14e-11 61.2
MS.gene008153.t1 MTR_7g105570 27.419 186 120 7 96 272 46 225 7.19e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008153.t1 AT5G06060 65.637 259 89 0 13 271 5 263 1.57e-132 375
MS.gene008153.t1 AT2G29260 61.719 256 98 0 13 268 64 319 2.98e-120 347
MS.gene008153.t1 AT2G29290 62.016 258 96 1 13 268 3 260 1.70e-118 340
MS.gene008153.t1 AT1G07440 60.853 258 98 2 13 268 8 264 8.19e-118 338
MS.gene008153.t1 AT1G07450 62.205 254 95 1 13 266 4 256 3.04e-117 337
MS.gene008153.t1 AT2G29150 59.848 264 103 2 7 268 6 268 2.76e-115 332
MS.gene008153.t1 AT2G29360 59.470 264 105 1 7 268 6 269 2.24e-114 330
MS.gene008153.t1 AT2G29350 61.265 253 97 1 14 266 12 263 1.08e-112 325
MS.gene008153.t1 AT2G29340 56.923 260 110 1 13 270 3 262 3.08e-111 322
MS.gene008153.t1 AT2G29340 57.364 258 108 1 13 268 3 260 2.10e-110 321
MS.gene008153.t1 AT2G29370 58.462 260 108 0 7 266 6 265 4.09e-110 319
MS.gene008153.t1 AT2G29330 58.527 258 105 1 13 268 3 260 5.74e-110 318
MS.gene008153.t1 AT2G29300 57.915 259 106 2 13 268 3 261 1.31e-108 315
MS.gene008153.t1 AT2G29310 57.364 258 108 1 13 268 3 260 8.79e-108 313
MS.gene008153.t1 AT2G30670 58.661 254 105 0 13 266 3 256 2.83e-107 311
MS.gene008153.t1 AT2G29320 58.175 263 95 4 15 268 11 267 3.02e-107 311
MS.gene008153.t1 AT2G29290 60.504 238 92 1 33 268 6 243 3.01e-105 306
MS.gene008153.t1 AT2G29300 57.312 253 107 1 13 264 3 255 1.33e-104 306
MS.gene008153.t1 AT2G29350 60.444 225 88 1 42 266 2 225 4.21e-97 285
MS.gene008153.t1 AT2G29320 56.735 245 91 4 33 268 11 249 4.75e-96 283
MS.gene008153.t1 AT2G29310 55.882 238 103 1 33 268 21 258 1.28e-94 279
MS.gene008153.t1 AT2G29350 63.212 193 71 0 14 206 12 204 6.57e-88 261
MS.gene008153.t1 AT2G29340 56.186 194 85 0 13 206 3 196 1.17e-80 242
MS.gene008153.t1 AT2G29340 56.186 194 85 0 13 206 3 196 1.17e-80 242
MS.gene008153.t1 AT2G29310 57.732 194 82 0 13 206 3 196 1.48e-79 239
MS.gene008153.t1 AT2G29320 57.592 191 80 1 15 204 11 201 1.42e-77 234
MS.gene008153.t1 AT1G07440 59.877 162 65 0 13 174 8 169 6.22e-71 216
MS.gene008153.t1 AT2G29310 55.747 174 77 0 33 206 21 194 1.95e-66 206
MS.gene008153.t1 AT2G29170 63.333 90 33 0 14 103 13 102 4.25e-38 130
MS.gene008153.t1 AT5G18210 33.721 258 154 8 16 261 7 259 2.90e-31 117
MS.gene008153.t1 AT4G13180 33.083 266 164 7 7 263 3 263 4.15e-30 114
MS.gene008153.t1 AT3G03980 32.103 271 163 8 6 260 3 268 5.37e-30 114
MS.gene008153.t1 AT4G05530 32.114 246 162 5 17 261 9 250 1.11e-29 112
MS.gene008153.t1 AT1G24360 30.894 246 159 5 23 263 80 319 3.92e-28 110
MS.gene008153.t1 AT5G18210 33.750 240 142 8 16 243 7 241 6.29e-27 106
MS.gene008153.t1 AT1G63380 29.344 259 171 8 17 265 17 273 1.57e-23 97.1
MS.gene008153.t1 AT1G63380 29.344 259 171 8 17 265 15 271 1.63e-23 97.1
MS.gene008153.t1 AT1G63380 29.344 259 171 8 17 265 19 275 1.99e-23 97.1
MS.gene008153.t1 AT3G04000 31.008 258 162 8 17 260 14 269 2.61e-23 96.3
MS.gene008153.t1 AT1G63380 29.344 259 171 8 17 265 22 278 2.73e-23 96.7
MS.gene008153.t1 AT3G46170 28.016 257 176 6 17 265 26 281 1.84e-22 94.4
MS.gene008153.t1 AT1G54870 30.859 256 164 7 17 263 82 333 2.26e-22 94.7
MS.gene008153.t1 AT1G54870 30.859 256 164 7 17 263 84 335 2.46e-22 94.7
MS.gene008153.t1 AT1G62610 27.132 258 178 7 17 265 14 270 2.81e-22 93.6
MS.gene008153.t1 AT1G62610 27.132 258 178 7 17 265 19 275 2.93e-22 93.6
MS.gene008153.t1 AT1G62610 27.132 258 178 7 17 265 13 269 2.93e-22 93.6
MS.gene008153.t1 AT1G62610 27.132 258 178 7 17 265 15 271 3.03e-22 93.6
MS.gene008153.t1 AT3G55310 28.682 258 173 8 17 265 17 272 5.47e-22 92.8
MS.gene008153.t1 AT3G55290 28.736 261 169 9 17 265 18 273 9.35e-22 92.4
MS.gene008153.t1 AT3G55290 28.736 261 169 9 17 265 17 272 1.04e-21 92.0
MS.gene008153.t1 AT3G51680 28.571 266 168 7 17 262 32 295 2.55e-21 91.7
MS.gene008153.t1 AT3G05260 29.020 255 169 6 17 263 37 287 2.83e-20 88.6
MS.gene008153.t1 AT2G17845 26.515 264 176 9 17 266 47 306 1.54e-19 86.7
MS.gene008153.t1 AT3G12800 26.482 253 174 6 19 261 12 262 2.17e-19 86.3
MS.gene008153.t1 AT3G26760 27.099 262 172 6 17 264 36 292 2.18e-19 86.3
MS.gene008153.t1 AT4G03140 26.172 256 175 7 17 262 78 329 6.48e-17 79.7
MS.gene008153.t1 AT1G52340 30.153 262 163 9 17 262 18 275 1.54e-16 78.2
MS.gene008153.t1 AT3G26770 27.692 260 171 6 17 264 41 295 3.99e-16 77.0
MS.gene008153.t1 AT3G42960 27.969 261 156 8 22 262 13 261 6.33e-15 73.2
MS.gene008153.t1 AT2G47130 25.869 259 169 7 17 263 6 253 1.90e-14 71.6
MS.gene008153.t1 AT3G06060 28.125 192 129 3 22 209 42 228 6.14e-14 71.2
MS.gene008153.t1 AT3G06060 28.125 192 129 3 22 209 59 245 7.51e-14 70.9
MS.gene008153.t1 AT5G04900 29.064 203 134 7 2 198 63 261 3.60e-13 68.9
MS.gene008153.t1 AT3G47350 28.037 214 143 6 16 224 43 250 5.90e-13 68.2
MS.gene008153.t1 AT3G29260 26.616 263 170 7 12 263 3 253 6.37e-13 67.4
MS.gene008153.t1 AT3G47350 28.037 214 143 6 16 224 43 250 6.47e-13 67.8
MS.gene008153.t1 AT2G47140 26.996 263 170 10 12 263 3 254 2.87e-12 65.5
MS.gene008153.t1 AT5G19200 26.471 204 141 3 23 222 41 239 3.08e-12 66.2
MS.gene008153.t1 AT1G24470 27.228 202 136 6 13 207 46 243 1.17e-11 64.3
MS.gene008153.t1 AT3G03330 25.628 199 139 4 8 199 32 228 1.69e-11 63.9
MS.gene008153.t1 AT1G49670 29.126 206 129 8 19 210 6 208 2.11e-11 64.3
MS.gene008153.t1 AT1G49670 29.126 206 129 8 19 210 6 208 2.18e-11 64.3
MS.gene008153.t1 AT5G10050 29.508 183 122 4 23 204 11 187 9.39e-11 61.2

Find 63 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATAAGGCTCTCTCTTT+GGG 0.173020 3.4:-69208438 None:intergenic
GTTGAATAAGGCTCTCTCTT+TGG 0.264119 3.4:-69208439 None:intergenic
ACCGGTGGCACTCGTGGAAT+CGG 0.347184 3.4:+69207930 MS.gene008153:CDS
TGGAAGCCACTTGTTGAATA+AGG 0.356666 3.4:-69208451 None:intergenic
TATCCTCTTCTAAAAGAATC+TGG 0.356990 3.4:+69209372 MS.gene008153:CDS
ATGTTCTACAAGTGGGGTTC+TGG 0.364231 3.4:-69209661 None:intergenic
ACCCGAAGAGGTCTCCTCTT+TGG 0.381912 3.4:+69210294 MS.gene008153:CDS
GTGAATGATCTTGTTGCATT+TGG 0.387546 3.4:+69208302 MS.gene008153:CDS
GTGTTCATTTCATCTGTTGC+AGG 0.395569 3.4:+69209408 MS.gene008153:CDS
GGACAAGTTATTTGTGTTGA+TGG 0.402356 3.4:+69210355 MS.gene008153:CDS
ATCTCATGTAATTCTCATGC+TGG 0.418009 3.4:-69210404 None:intergenic
CTTCTAAAAGAATCTGGAAA+TGG 0.429287 3.4:+69209378 MS.gene008153:CDS
GTTGCAGGTGTGGTTAGCTT+AGG 0.435339 3.4:+69209423 MS.gene008153:CDS
CACCAAAGAGGAGACCTCTT+CGG 0.436189 3.4:-69210296 None:intergenic
TCAATAAGTGTTTACAAGAA+TGG 0.436812 3.4:+69208363 MS.gene008153:CDS
GATATGCAAGTTGGCACAAA+TGG 0.442938 3.4:-69209353 None:intergenic
TTTACAAGAATGGCAAAGTC+AGG 0.444926 3.4:+69208373 MS.gene008153:CDS
CAAGTGGCTTCCACTTTCAA+TGG 0.445054 3.4:+69208461 MS.gene008153:CDS
GGTTCGGCAATGCGCTTAAG+AGG 0.448812 3.4:-69210274 None:intergenic
AGAGGAGACCTCTTCGGGTT+CGG 0.459507 3.4:-69210290 None:intergenic
TGCAACAAGATCATTCACTA+TGG 0.461943 3.4:-69208297 None:intergenic
ACCAAAGAGGAGACCTCTTC+GGG 0.477426 3.4:-69210295 None:intergenic
GGAATGACAGCACTTGTAAC+CGG 0.485759 3.4:+69207912 MS.gene008153:CDS
CTACTGTCCACACTTGTTCA+AGG 0.488543 3.4:+69208327 MS.gene008153:CDS
TCAGCAGTGTACTCAATGGT+TGG 0.489827 3.4:-69209288 None:intergenic
ATTTCAAAATCAAAAGTTTG+TGG 0.490723 3.4:+69210231 MS.gene008153:CDS
GTTGTCTATTGCCCATTCAC+AGG 0.493489 3.4:-69209607 None:intergenic
AATGGGCAATAGACAACATA+AGG 0.496335 3.4:+69209613 MS.gene008153:CDS
GGTGTGGTTAGCTTAGGTAC+TGG 0.499150 3.4:+69209429 MS.gene008153:CDS
TTCTTCAGCAGTGTACTCAA+TGG 0.499815 3.4:-69209292 None:intergenic
GACAAGTTATTTGTGTTGAT+GGG 0.502183 3.4:+69210356 MS.gene008153:CDS
CCGGTGGCACTCGTGGAATC+GGG 0.504977 3.4:+69207931 MS.gene008153:CDS
GTTGAGCTTGCCATTGAAAG+TGG 0.515168 3.4:-69208471 None:intergenic
CATTTCATCTGTTGCAGGTG+TGG 0.515366 3.4:+69209413 MS.gene008153:CDS
CTTGTAACCGGTGGCACTCG+TGG 0.527392 3.4:+69207924 MS.gene008153:CDS
CCCGATTCCACGAGTGCCAC+CGG 0.529955 3.4:-69207931 None:intergenic
GGACAGAGATGGTCTCTCAC+TGG 0.538298 3.4:+69207891 MS.gene008153:CDS
TCTGTCTACGCAGCAAGTAA+AGG 0.541483 3.4:+69209453 MS.gene008153:CDS
CCTGCTGCATCTTACATCAC+TGG 0.543937 3.4:+69210334 MS.gene008153:CDS
AAATTACATGTTCTACAAGT+GGG 0.546415 3.4:-69209668 None:intergenic
TTAGAAGAGGATATGCAAGT+TGG 0.555015 3.4:-69209362 None:intergenic
AGTGGGGTTCTGGTTGCCCA+TGG 0.559899 3.4:-69209651 None:intergenic
GAGCAATTGTGTTGTACCAT+GGG 0.560513 3.4:+69209635 MS.gene008153:CDS
GAAACACATAGCAGCAGAGG+TGG 0.561832 3.4:+69207870 MS.gene008153:CDS
AGAGAGCCTTATTCAACAAG+TGG 0.562362 3.4:+69208445 MS.gene008153:CDS
AAAATTACATGTTCTACAAG+TGG 0.573526 3.4:-69209669 None:intergenic
TCTCATGTAATTCTCATGCT+GGG 0.575912 3.4:-69210403 None:intergenic
GGAGCAATTGTGTTGTACCA+TGG 0.576420 3.4:+69209634 MS.gene008153:CDS
GCGCATTGCCGAACCCGAAG+AGG 0.578494 3.4:+69210282 MS.gene008153:CDS
GCAGCAGAGGTGGACAGAGA+TGG 0.586161 3.4:+69207880 MS.gene008153:CDS
GTAGAAACACATAGCAGCAG+AGG 0.588040 3.4:+69207867 MS.gene008153:CDS
TGAATAAGGCTCTCTCTTTG+GGG 0.599329 3.4:-69208437 None:intergenic
CAAGTTATTTGTGTTGATGG+GGG 0.602087 3.4:+69210358 MS.gene008153:CDS
ATGCTTTAGGTAAATAACGT+GGG 0.610168 3.4:+69209249 MS.gene008153:intron
ATTCAGTCCTTGAACAAGTG+TGG 0.613741 3.4:-69208334 None:intergenic
TTACAAGAATGGCAAAGTCA+GGG 0.621457 3.4:+69208374 MS.gene008153:CDS
CCAGTGATGTAAGATGCAGC+AGG 0.631919 3.4:-69210334 None:intergenic
ACAAGTTATTTGTGTTGATG+GGG 0.632558 3.4:+69210357 MS.gene008153:CDS
AAGATGCAGCAGGTAAGCAA+AGG 0.634488 3.4:-69210324 None:intergenic
TATGCTTTAGGTAAATAACG+TGG 0.635045 3.4:+69209248 MS.gene008153:intron
AATTACATGTTCTACAAGTG+GGG 0.636533 3.4:-69209667 None:intergenic
GCAAAGGAAAGTCACCAAAG+AGG 0.638884 3.4:-69210308 None:intergenic
ATGACAGCACTTGTAACCGG+TGG 0.672686 3.4:+69207915 MS.gene008153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAGTAAGTT+AGG - chr3.4:69209702-69209721 None:intergenic 10.0%
!! AAAAAAAAAAAAGTAAGTTA+GGG - chr3.4:69209701-69209720 None:intergenic 10.0%
!! TATTATGTATCCATATTTAT+TGG - chr3.4:69209767-69209786 None:intergenic 15.0%
!!! AAAAGATAATGAAGTATTTT+TGG - chr3.4:69208049-69208068 None:intergenic 15.0%
!!! CTTACTTTTTTTTTTTTTGA+AGG + chr3.4:69209704-69209723 MS.gene008153:intron 15.0%
!!! GACACTAATTTTAAAAAAAA+AGG - chr3.4:69208075-69208094 None:intergenic 15.0%
!!! TATTTACATTTTTTCATTCA+TGG + chr3.4:69210070-69210089 MS.gene008153:intron 15.0%
!! AAAATACACATAAAACTCAA+AGG - chr3.4:69209843-69209862 None:intergenic 20.0%
!! ATTTATAACTCTTTCGTTAT+TGG - chr3.4:69208873-69208892 None:intergenic 20.0%
!! ATTTCAAAATCAAAAGTTTG+TGG + chr3.4:69210231-69210250 MS.gene008153:CDS 20.0%
!! TAATCAGCTTACAAAAAATT+TGG + chr3.4:69209584-69209603 MS.gene008153:CDS 20.0%
!!! TTTTTTGTTCAAACTAACAA+TGG + chr3.4:69210156-69210175 MS.gene008153:intron 20.0%
! AAAATTACATGTTCTACAAG+TGG - chr3.4:69209672-69209691 None:intergenic 25.0%
! AAATAATGAAGTAAGAGAGA+AGG - chr3.4:69207970-69207989 None:intergenic 25.0%
! AAATTACATGTTCTACAAGT+GGG - chr3.4:69209671-69209690 None:intergenic 25.0%
! AATAATGAAGTAAGAGAGAA+GGG - chr3.4:69207969-69207988 None:intergenic 25.0%
! TCAATAAGTGTTTACAAGAA+TGG + chr3.4:69208363-69208382 MS.gene008153:CDS 25.0%
! TCTGCTTATGATTGATTATA+TGG + chr3.4:69209982-69210001 MS.gene008153:intron 25.0%
! TTGAAGGAAACTTACTTATT+TGG + chr3.4:69209720-69209739 MS.gene008153:intron 25.0%
! TTTGCATGAATTTATGCTTT+AGG + chr3.4:69209236-69209255 MS.gene008153:intron 25.0%
!! ACTAGACATATCAAAAGTTT+TGG - chr3.4:69209096-69209115 None:intergenic 25.0%
!! ATGTTATCTTTTCTCAATAG+TGG - chr3.4:69208706-69208725 None:intergenic 25.0%
!! ATTCATTTGACACTTTGTTT+AGG + chr3.4:69208270-69208289 MS.gene008153:intron 25.0%
!! CAATGTTGACTGTTTATTTA+TGG + chr3.4:69208548-69208567 MS.gene008153:intron 25.0%
!! TAAACAAAGTGTCAAATGAA+TGG - chr3.4:69208271-69208290 None:intergenic 25.0%
!!! CATATTTATTGGTTCTATGT+GGG - chr3.4:69209756-69209775 None:intergenic 25.0%
!!! CGTTTAAATGTTTTTACACA+CGG + chr3.4:69208094-69208113 MS.gene008153:intron 25.0%
AAAAAATTTGGCCTGTGAAT+GGG + chr3.4:69209596-69209615 MS.gene008153:CDS 30.0%
AACTGAAGTTTGAATGACAT+AGG + chr3.4:69208591-69208610 MS.gene008153:intron 30.0%
AATTACATGTTCTACAAGTG+GGG - chr3.4:69209670-69209689 None:intergenic 30.0%
AATTGCAGCTGAAAATTCAA+TGG - chr3.4:69209565-69209584 None:intergenic 30.0%
ACTGAAGTTTGAATGACATA+GGG + chr3.4:69208592-69208611 MS.gene008153:intron 30.0%
AGTAAAGCTCTTTAGTAACA+CGG - chr3.4:69208177-69208196 None:intergenic 30.0%
ATAATGAAGTAAGAGAGAAG+GGG - chr3.4:69207968-69207987 None:intergenic 30.0%
ATTGTGCCAAACGTATAAAA+GGG + chr3.4:69208510-69208529 MS.gene008153:intron 30.0%
CAACAAAAATCAGGAAGAAA+CGG - chr3.4:69209031-69209050 None:intergenic 30.0%
CCACATAGAACCAATAAATA+TGG + chr3.4:69209754-69209773 MS.gene008153:intron 30.0%
CTACATATGAATAATGATGC+AGG - chr3.4:69208009-69208028 None:intergenic 30.0%
GTTACATTCGAACAATTGTT+AGG + chr3.4:69208145-69208164 MS.gene008153:intron 30.0%
TAAAACAGTGACTACTTGTA+AGG - chr3.4:69208917-69208936 None:intergenic 30.0%
TAGATCCAAATGATGAATTC+AGG - chr3.4:69209057-69209076 None:intergenic 30.0%
TCTCAATTATGTTGTAGTGT+TGG - chr3.4:69208121-69208140 None:intergenic 30.0%
TGAGAAAAGAGTGATAACAA+TGG + chr3.4:69208661-69208680 MS.gene008153:intron 30.0%
TGATTCCTGAATTCATCATT+TGG + chr3.4:69209049-69209068 MS.gene008153:intron 30.0%
TTCAGGAATCAACAAAAATC+AGG - chr3.4:69209040-69209059 None:intergenic 30.0%
! ACAAGTTATTTGTGTTGATG+GGG + chr3.4:69210357-69210376 MS.gene008153:CDS 30.0%
! ATGCTTTAGGTAAATAACGT+GGG + chr3.4:69209249-69209268 MS.gene008153:intron 30.0%
! ATTCCTATCTTCAAGTTCAA+TGG + chr3.4:69210105-69210124 MS.gene008153:intron 30.0%
! CTTCTAAAAGAATCTGGAAA+TGG + chr3.4:69209378-69209397 MS.gene008153:CDS 30.0%
! GAAAGTTAGTCTATTTGTTC+CGG + chr3.4:69208818-69208837 MS.gene008153:intron 30.0%
! GACAAGTTATTTGTGTTGAT+GGG + chr3.4:69210356-69210375 MS.gene008153:CDS 30.0%
! GTAAGTACATTGTTTAGAGT+CGG + chr3.4:69209114-69209133 MS.gene008153:intron 30.0%
! TATCCTCTTCTAAAAGAATC+TGG + chr3.4:69209372-69209391 MS.gene008153:CDS 30.0%
! TATGCTTTAGGTAAATAACG+TGG + chr3.4:69209248-69209267 MS.gene008153:intron 30.0%
!! CCATATTTATTGGTTCTATG+TGG - chr3.4:69209757-69209776 None:intergenic 30.0%
!! TTTCCAGATTCTTTTAGAAG+AGG - chr3.4:69209378-69209397 None:intergenic 30.0%
AAGTTTGAATGACATAGGGT+TGG + chr3.4:69208596-69208615 MS.gene008153:intron 35.0%
AATGGGCAATAGACAACATA+AGG + chr3.4:69209613-69209632 MS.gene008153:CDS 35.0%
CAAAAAATTTGGCCTGTGAA+TGG + chr3.4:69209595-69209614 MS.gene008153:CDS 35.0%
CATTGTGCCAAACGTATAAA+AGG + chr3.4:69208509-69208528 MS.gene008153:intron 35.0%
GAGTGATAACAATGGTAAAG+TGG + chr3.4:69208669-69208688 MS.gene008153:intron 35.0%
GGACAAGTTATTTGTGTTGA+TGG + chr3.4:69210355-69210374 MS.gene008153:CDS 35.0%
GTGAATGATCTTGTTGCATT+TGG + chr3.4:69208302-69208321 MS.gene008153:CDS 35.0%
TCATAAGCAGACATTACAGA+TGG - chr3.4:69209973-69209992 None:intergenic 35.0%
TCGTTATTGGACTTGATGTT+TGG - chr3.4:69208860-69208879 None:intergenic 35.0%
TGCAACAAGATCATTCACTA+TGG - chr3.4:69208300-69208319 None:intergenic 35.0%
TGGATAATTTCGTAGTTGAC+CGG - chr3.4:69208840-69208859 None:intergenic 35.0%
TGTCCATTGAACTTGAAGAT+AGG - chr3.4:69210111-69210130 None:intergenic 35.0%
TTACAAGAATGGCAAAGTCA+GGG + chr3.4:69208374-69208393 MS.gene008153:CDS 35.0%
TTAGAAGAGGATATGCAAGT+TGG - chr3.4:69209365-69209384 None:intergenic 35.0%
TTGAATAAGGCTCTCTCTTT+GGG - chr3.4:69208441-69208460 None:intergenic 35.0%
TTTACAAGAATGGCAAAGTC+AGG + chr3.4:69208373-69208392 MS.gene008153:CDS 35.0%
! CAAAGTGTCAAATGAATGGA+TGG - chr3.4:69208267-69208286 None:intergenic 35.0%
! CAAGTTATTTGTGTTGATGG+GGG + chr3.4:69210358-69210377 MS.gene008153:CDS 35.0%
! CTTAGACCCTTTTATACGTT+TGG - chr3.4:69208519-69208538 None:intergenic 35.0%
!! TGGACTAGCAGTAAATTGTT+AGG + chr3.4:69210002-69210021 MS.gene008153:intron 35.0%
AGTCGGATGTCATTCAACAA+TGG + chr3.4:69209131-69209150 MS.gene008153:intron 40.0%
ATTCAGTCCTTGAACAAGTG+TGG - chr3.4:69208337-69208356 None:intergenic 40.0%
GAGCAATTGTGTTGTACCAT+GGG + chr3.4:69209635-69209654 MS.gene008153:CDS 40.0%
GGAGATAGTAAATGCTTGAG+TGG + chr3.4:69208617-69208636 MS.gene008153:intron 40.0%
GTCGGATGTCATTCAACAAT+GGG + chr3.4:69209132-69209151 MS.gene008153:intron 40.0%
GTGTTCATTTCATCTGTTGC+AGG + chr3.4:69209408-69209427 MS.gene008153:CDS 40.0%
GTTGAATAAGGCTCTCTCTT+TGG - chr3.4:69208442-69208461 None:intergenic 40.0%
TGAATAAGGCTCTCTCTTTG+GGG - chr3.4:69208440-69208459 None:intergenic 40.0%
TTCTTCAGCAGTGTACTCAA+TGG - chr3.4:69209295-69209314 None:intergenic 40.0%
! ATGGCAAAGTCAGGGATTTT+CGG + chr3.4:69208382-69208401 MS.gene008153:CDS 40.0%
! GATATGCAAGTTGGCACAAA+TGG - chr3.4:69209356-69209375 None:intergenic 40.0%
! TCGATCGATGTTTAGAGTTG+AGG + chr3.4:69208994-69209013 MS.gene008153:intron 40.0%
! TGGAAGCCACTTGTTGAATA+AGG - chr3.4:69208454-69208473 None:intergenic 40.0%
!! AGAGAGCCTTATTCAACAAG+TGG + chr3.4:69208445-69208464 MS.gene008153:CDS 40.0%
AAGATGCAGCAGGTAAGCAA+AGG - chr3.4:69210327-69210346 None:intergenic 45.0%
ATGTTCTACAAGTGGGGTTC+TGG - chr3.4:69209664-69209683 None:intergenic 45.0%
CAAGTGGCTTCCACTTTCAA+TGG + chr3.4:69208461-69208480 MS.gene008153:CDS 45.0%
CATTTCATCTGTTGCAGGTG+TGG + chr3.4:69209413-69209432 MS.gene008153:CDS 45.0%
CTACTGTCCACACTTGTTCA+AGG + chr3.4:69208327-69208346 MS.gene008153:CDS 45.0%
GATGGGGGATTAACTGTGTT+TGG + chr3.4:69210373-69210392 MS.gene008153:CDS 45.0%
GCAAAGGAAAGTCACCAAAG+AGG - chr3.4:69210311-69210330 None:intergenic 45.0%
GGAATGACAGCACTTGTAAC+CGG + chr3.4:69207912-69207931 MS.gene008153:CDS 45.0%
GGAGCAATTGTGTTGTACCA+TGG + chr3.4:69209634-69209653 MS.gene008153:CDS 45.0%
GTAGAAACACATAGCAGCAG+AGG + chr3.4:69207867-69207886 MS.gene008153:CDS 45.0%
GTTGAGCTTGCCATTGAAAG+TGG - chr3.4:69208474-69208493 None:intergenic 45.0%
GTTGTCTATTGCCCATTCAC+AGG - chr3.4:69209610-69209629 None:intergenic 45.0%
TCAGCAGTGTACTCAATGGT+TGG - chr3.4:69209291-69209310 None:intergenic 45.0%
TCTGTCTACGCAGCAAGTAA+AGG + chr3.4:69209453-69209472 MS.gene008153:CDS 45.0%
! AGTCAGGGATTTTCGGTTAC+TGG + chr3.4:69208389-69208408 MS.gene008153:CDS 45.0%
! GGGATTTTCGGTTACTGGAT+CGG + chr3.4:69208394-69208413 MS.gene008153:CDS 45.0%
!! CAAAAATTAAAAAAAAATAT+TGG - chr3.4:69210206-69210225 None:intergenic 5.0%
ACCAAAGAGGAGACCTCTTC+GGG - chr3.4:69210298-69210317 None:intergenic 50.0%
ATGACAGCACTTGTAACCGG+TGG + chr3.4:69207915-69207934 MS.gene008153:CDS 50.0%
CACCAAAGAGGAGACCTCTT+CGG - chr3.4:69210299-69210318 None:intergenic 50.0%
CCAGTGATGTAAGATGCAGC+AGG - chr3.4:69210337-69210356 None:intergenic 50.0%
CCTGCTGCATCTTACATCAC+TGG + chr3.4:69210334-69210353 MS.gene008153:CDS 50.0%
GAAACACATAGCAGCAGAGG+TGG + chr3.4:69207870-69207889 MS.gene008153:CDS 50.0%
!! GGTGTGGTTAGCTTAGGTAC+TGG + chr3.4:69209429-69209448 MS.gene008153:CDS 50.0%
!! GTTGCAGGTGTGGTTAGCTT+AGG + chr3.4:69209423-69209442 MS.gene008153:CDS 50.0%
ACCCGAAGAGGTCTCCTCTT+TGG + chr3.4:69210294-69210313 MS.gene008153:CDS 55.0%
GGACAGAGATGGTCTCTCAC+TGG + chr3.4:69207891-69207910 MS.gene008153:CDS 55.0%
GGTTCGGCAATGCGCTTAAG+AGG - chr3.4:69210277-69210296 None:intergenic 55.0%
! AGAGGAGACCTCTTCGGGTT+CGG - chr3.4:69210293-69210312 None:intergenic 55.0%
ACCGGTGGCACTCGTGGAAT+CGG + chr3.4:69207930-69207949 MS.gene008153:CDS 60.0%
CTTGTAACCGGTGGCACTCG+TGG + chr3.4:69207924-69207943 MS.gene008153:CDS 60.0%
GCAGCAGAGGTGGACAGAGA+TGG + chr3.4:69207880-69207899 MS.gene008153:CDS 60.0%
! AGTGGGGTTCTGGTTGCCCA+TGG - chr3.4:69209654-69209673 None:intergenic 60.0%
CCCGATTCCACGAGTGCCAC+CGG - chr3.4:69207934-69207953 None:intergenic 65.0%
CCGGTGGCACTCGTGGAATC+GGG + chr3.4:69207931-69207950 MS.gene008153:CDS 65.0%
GCGCATTGCCGAACCCGAAG+AGG + chr3.4:69210282-69210301 MS.gene008153:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 69207858 69210423 69207858 ID=MS.gene008153
chr3.4 mRNA 69207858 69210423 69207858 ID=MS.gene008153.t1;Parent=MS.gene008153
chr3.4 exon 69207858 69207952 69207858 ID=MS.gene008153.t1.exon1;Parent=MS.gene008153.t1
chr3.4 CDS 69207858 69207952 69207858 ID=cds.MS.gene008153.t1;Parent=MS.gene008153.t1
chr3.4 exon 69208292 69208499 69208292 ID=MS.gene008153.t1.exon2;Parent=MS.gene008153.t1
chr3.4 CDS 69208292 69208499 69208292 ID=cds.MS.gene008153.t1;Parent=MS.gene008153.t1
chr3.4 exon 69209258 69209474 69209258 ID=MS.gene008153.t1.exon3;Parent=MS.gene008153.t1
chr3.4 CDS 69209258 69209474 69209258 ID=cds.MS.gene008153.t1;Parent=MS.gene008153.t1
chr3.4 exon 69209577 69209683 69209577 ID=MS.gene008153.t1.exon4;Parent=MS.gene008153.t1
chr3.4 CDS 69209577 69209683 69209577 ID=cds.MS.gene008153.t1;Parent=MS.gene008153.t1
chr3.4 exon 69210229 69210423 69210229 ID=MS.gene008153.t1.exon5;Parent=MS.gene008153.t1
chr3.4 CDS 69210229 69210423 69210229 ID=cds.MS.gene008153.t1;Parent=MS.gene008153.t1
Gene Sequence

>MS.gene008153

ATGGCAGCAGTAGAAACACATAGCAGCAGAGGTGGACAGAGATGGTCTCTCACTGGAATGACAGCACTTGTAACCGGTGGCACTCGTGGAATCGGGTTTTTATCACTCCCCTTCTCTCTTACTTCATTATTTATCTTATCTTCAACATCCTGCATCATTATTCATATGTAGTCTTTATTTTGATCTAGCCAAAAATACTTCATTATCTTTTACTCCTTTTTTTTTAAAATTAGTGTCGTTTAAATGTTTTTACACACGGACCAACACTACAACATAATTGAGATCTAGTTACATTCGAACAATTGTTAGGTAAAATCCGTGTTACTAAAGAGCTTTACTAACATTTTATAGTATTATATATCAGTAAGTTATTCAGATGACACTTTTATTAAGAATAGATTTTAATCCATCCATTCATTTGACACTTTGTTTAGGCATGCCATAGTGAATGATCTTGTTGCATTTGGTGCTACTGTCCACACTTGTTCAAGGACTGAATCAGAGCTCAATAAGTGTTTACAAGAATGGCAAAGTCAGGGATTTTCGGTTACTGGATCGGTCTGTGATGTAAGCTCTCGACCCCAAAGAGAGAGCCTTATTCAACAAGTGGCTTCCACTTTCAATGGCAAGCTCAACATCTTTGTAAGTACTCATTGTGCCAAACGTATAAAAGGGTCTAAGAAAAACATACAATGTTGACTGTTTATTTATGGATCTATGCAGAAAACTATTGAACTGAAGTTTGAATGACATAGGGTTGGAGATAGTAAATGCTTGAGTGGTAGTAGTAGTAGTAGTAGTATTGAGAAAAGAGTGATAACAATGGTAAAGTGGATATGTCTGTGCCACTATTGAGAAAAGATAACATCTTATAAGAGTTTATGAAAAGTAATATTTTTTATCTTACAAGTTAATTTTGTAAGAATAAGTTAGACTCGTTATAAAAATCTAATATAGTATGAAAGTTAGTCTATTTGTTCCGGTCAACTACGAAATTATCCAAACATCAAGTCCAATAACGAAAGAGTTATAAATACTAAACAATGACATCTCTCGCCTTACAAGTAGTCACTGTTTTACTTTAAATACTAAACAATGTATCTGCGCTTAAGATTTTTGTATAGAAATTATGCTAGTCGATCGATGTTTAGAGTTGAGGTCAACAACAAACCGTTTCTTCCTGATTTTTGTTGATTCCTGAATTCATCATTTGGATCTAGTTATTTAATATTTCTCCAAAACTTTTGATATGTCTAGTAAGTACATTGTTTAGAGTCGGATGTCATTCAACAATGGGTGCAGTCTGCAAATGATGCTTATGCGATGAGATAAATTTACAGCAGAAATTAATGAATCACTTTGCTATGAATCATACATATTTGCATGAATTTATGCTTTAGGTAAATAACGTGGGATCAAACTTTAGAAAGCCAACCATTGAGTACACTGCTGAAGAATATTCAGAGCTAATGACAATTAATTTAGACTCATCATTCCATTTGTGCCAACTTGCATATCCTCTTCTAAAAGAATCTGGAAATGGTAGCATTGTGTTCATTTCATCTGTTGCAGGTGTGGTTAGCTTAGGTACTGGATCTGTCTACGCAGCAAGTAAAGGTGAGTGCTACACTCATTAGTTCTTTTATTTTTTTCATGTAAATGTGATTAATAAAATGTTACGATTAAACGACAATTGTGTTTCATCCATTGAATTTTCAGCTGCAATTAATCAGCTTACAAAAAATTTGGCCTGTGAATGGGCAATAGACAACATAAGGAGCAATTGTGTTGTACCATGGGCAACCAGAACCCCACTTGTAGAACATGTAATTTTCTTGCTCCCTAACTTACTTTTTTTTTTTTTGAAGGAAACTTACTTATTTGGTTGATTGAATCCCACATAGAACCAATAAATATGGATACATAATAATACTCAAAAAAATTATATTTAGACGTCAAAATTTGATGTCAAATACGAGTGTCCTTTGAGTTTTATGTGTATTTTTAAAATGTAAGTTGTAGAAAATAAAAAAGAAATAGAGATAAACATATACGATGTCGCATATAGTAACTGTATTTTGATGTTCAAATATAATTTCTTTAGTATTATTACCATCTGTAATGTCTGCTTATGATTGATTATATGGACTAGCAGTAAATTGTTAGGATAGCATAAATTTAAATATAGTGTTTTTGAATTGATTGTGTCTAATATTTACATTTTTTCATTCATGGAAATACATGACTATTCCTATCTTCAAGTTCAATGGACATTCTTCATACTCTATCAAGCAAATCTTTTTTGTTCAAACTAACAATGGATGTCTCTTGTCTATGTGTTTGATCCAATATTTTTTTTTAATTTTTGTAGTTATTTCAAAATCAAAAGTTTGTGGATGATATTATGTCTCGAACTCCTCTTAAGCGCATTGCCGAACCCGAAGAGGTCTCCTCTTTGGTGACTTTCCTTTGCTTACCTGCTGCATCTTACATCACTGGACAAGTTATTTGTGTTGATGGGGGATTAACTGTGTTTGGATTTCAACCCAGCATGAGAATTACATGA
Protein sequence

>MS.gene008153.t1

MAAVETHSSRGGQRWSLTGMTALVTGGTRGIGHAIVNDLVAFGATVHTCSRTESELNKCLQEWQSQGFSVTGSVCDVSSRPQRESLIQQVASTFNGKLNIFVNNVGSNFRKPTIEYTAEEYSELMTINLDSSFHLCQLAYPLLKESGNGSIVFISSVAGVVSLGTGSVYAASKAAINQLTKNLACEWAIDNIRSNCVVPWATRTPLVEHLFQNQKFVDDIMSRTPLKRIAEPEEVSSLVTFLCLPAASYITGQVICVDGGLTVFGFQPSMRIT