AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63307

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63307.t1 MTR_3g068070 95.539 269 12 0 1 269 1 269 0.0 530
MS.gene63307.t1 MTR_3g068085 87.879 264 32 0 1 264 1 264 1.45e-166 462
MS.gene63307.t1 MTR_3g068065 84.800 250 38 0 15 264 17 266 2.45e-159 444
MS.gene63307.t1 MTR_3g068155 73.359 259 69 0 6 264 10 268 1.35e-143 404
MS.gene63307.t1 MTR_3g068175 69.847 262 78 1 4 264 5 266 7.54e-137 387
MS.gene63307.t1 MTR_3g068175 68.797 266 78 2 4 264 5 270 1.61e-134 381
MS.gene63307.t1 MTR_4g064883 61.811 254 97 0 12 265 11 264 9.96e-124 353
MS.gene63307.t1 MTR_4g064887 58.397 262 109 0 1 262 1 262 2.01e-115 332
MS.gene63307.t1 MTR_7g063320 57.736 265 108 1 1 261 1 265 2.85e-109 317
MS.gene63307.t1 MTR_7g063270 58.779 262 105 1 2 260 3 264 7.17e-108 313
MS.gene63307.t1 MTR_7g063260 57.795 263 107 1 2 260 3 265 4.00e-106 308
MS.gene63307.t1 MTR_7g063390 56.061 264 112 2 2 261 3 266 4.96e-104 303
MS.gene63307.t1 MTR_7g063330 57.414 263 109 2 1 260 1 263 5.46e-104 303
MS.gene63307.t1 MTR_7g063360 57.197 264 109 2 2 261 3 266 6.17e-104 303
MS.gene63307.t1 MTR_4g064883 62.626 198 74 0 12 209 11 208 2.53e-94 276
MS.gene63307.t1 MTR_4g064887 59.330 209 85 0 1 209 1 209 3.86e-90 266
MS.gene63307.t1 MTR_7g063270 48.855 262 82 3 2 260 3 215 1.45e-78 237
MS.gene63307.t1 MTR_4g132850 33.333 246 159 4 15 259 7 248 1.69e-35 127
MS.gene63307.t1 MTR_7g094510 33.202 253 157 7 15 259 6 254 7.74e-32 118
MS.gene63307.t1 MTR_7g094520 33.203 256 153 8 15 259 11 259 7.45e-31 115
MS.gene63307.t1 MTR_3g085740 31.301 246 158 6 21 261 81 320 4.58e-29 112
MS.gene63307.t1 MTR_7g071680 32.000 275 149 8 15 260 29 294 4.98e-29 112
MS.gene63307.t1 MTR_4g058810 31.034 261 167 9 14 264 10 267 1.41e-26 105
MS.gene63307.t1 MTR_4g058840 30.385 260 168 9 15 264 11 267 6.98e-25 100
MS.gene63307.t1 MTR_4g058820 30.385 260 168 9 15 264 11 267 6.98e-25 100
MS.gene63307.t1 MTR_7g083870 30.350 257 164 7 15 260 35 287 7.21e-25 100
MS.gene63307.t1 MTR_1196s0010 31.301 246 156 6 18 259 277 513 2.30e-23 99.4
MS.gene63307.t1 MTR_4g078630 30.303 264 155 9 15 260 19 271 2.99e-23 96.3
MS.gene63307.t1 MTR_4g052350 31.034 261 168 9 15 269 14 268 2.13e-22 93.6
MS.gene63307.t1 MTR_7g107670 28.077 260 173 6 11 260 29 284 4.54e-22 93.2
MS.gene63307.t1 MTR_4g052340 29.183 257 171 7 15 265 14 265 6.19e-22 92.4
MS.gene63307.t1 MTR_4g052320 29.297 256 171 7 15 264 8 259 8.74e-22 92.0
MS.gene63307.t1 MTR_8g442750 27.519 258 168 5 15 259 14 265 5.41e-21 90.5
MS.gene63307.t1 MTR_4g131740 31.835 267 150 9 15 262 40 293 1.68e-20 89.0
MS.gene63307.t1 MTR_4g052300 30.268 261 161 9 15 264 8 258 3.95e-20 87.4
MS.gene63307.t1 MTR_1g068955 30.769 260 163 9 15 263 14 267 6.02e-20 87.4
MS.gene63307.t1 MTR_3g020670 30.385 260 162 9 15 260 16 270 6.57e-20 87.0
MS.gene63307.t1 MTR_3g068075 76.000 50 11 1 123 171 1 50 4.89e-18 77.0
MS.gene63307.t1 MTR_7g105590 29.570 186 124 4 15 197 8 189 1.20e-17 79.3
MS.gene63307.t1 MTR_6g023990 28.044 271 170 11 1 259 5 262 1.34e-17 80.5
MS.gene63307.t1 MTR_1g051165 26.894 264 175 12 15 263 14 274 3.55e-17 79.7
MS.gene63307.t1 MTR_7g105460 28.137 263 177 7 6 261 4 261 3.73e-17 79.3
MS.gene63307.t1 MTR_7g105580 25.954 262 170 5 8 265 1 242 4.57e-17 79.0
MS.gene63307.t1 MTR_4g058830 31.977 172 110 6 98 264 44 213 5.48e-17 77.8
MS.gene63307.t1 MTR_1g082760 29.070 258 170 7 15 263 40 293 7.04e-17 79.0
MS.gene63307.t1 MTR_7g110140 29.961 257 170 8 11 261 2 254 9.72e-17 78.2
MS.gene63307.t1 MTR_6g023590 26.692 266 177 10 5 259 3 261 1.52e-16 77.4
MS.gene63307.t1 MTR_6g023610 27.715 267 173 9 5 259 3 261 5.74e-16 75.9
MS.gene63307.t1 MTR_2g090165 27.547 265 166 7 11 261 2 254 1.05e-15 75.1
MS.gene63307.t1 MTR_7g110130 27.481 262 171 9 11 261 2 255 1.80e-15 74.7
MS.gene63307.t1 MTR_8g089575 30.808 198 128 5 17 207 47 242 6.20e-15 73.6
MS.gene63307.t1 MTR_7g110160 28.846 260 151 9 11 261 2 236 1.17e-14 72.0
MS.gene63307.t1 MTR_6g023600 27.473 273 166 10 5 259 3 261 1.56e-14 72.0
MS.gene63307.t1 MTR_8g044240 29.064 203 128 6 15 205 69 267 3.25e-14 72.0
MS.gene63307.t1 MTR_4g134660 32.584 178 101 6 18 184 9 178 2.31e-13 68.2
MS.gene63307.t1 MTR_7g105570 29.143 175 115 4 94 261 46 218 3.17e-13 67.8
MS.gene63307.t1 MTR_3g113870 26.667 180 126 3 28 205 34 209 5.97e-13 67.0
MS.gene63307.t1 MTR_6g041980 32.515 163 91 5 11 168 60 208 6.85e-13 67.4
MS.gene63307.t1 MTR_6g088500 23.551 276 166 9 7 243 164 433 1.62e-12 67.4
MS.gene63307.t1 MTR_1275s0010 29.060 117 83 0 139 255 259 375 1.87e-12 67.4
MS.gene63307.t1 MTR_6g041980 32.515 163 91 5 11 168 60 208 2.40e-12 66.6
MS.gene63307.t1 MTR_6g023600 27.106 273 161 10 5 259 3 255 2.43e-12 65.5
MS.gene63307.t1 MTR_3g098470 27.347 245 124 6 12 204 29 271 2.33e-11 63.2
MS.gene63307.t1 MTR_1g031180 32.168 143 95 2 12 154 34 174 8.96e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63307.t1 AT5G06060 70.155 258 77 0 12 269 6 263 7.83e-138 389
MS.gene63307.t1 AT2G29260 63.241 253 93 0 12 264 65 317 5.70e-119 343
MS.gene63307.t1 AT2G29360 61.303 261 101 0 5 265 6 266 9.56e-115 331
MS.gene63307.t1 AT2G29290 61.024 254 99 0 12 265 4 257 5.13e-114 328
MS.gene63307.t1 AT1G07450 59.690 258 103 1 8 265 1 257 1.21e-112 325
MS.gene63307.t1 AT2G29150 60.920 261 101 1 5 265 6 265 3.85e-112 324
MS.gene63307.t1 AT1G07440 60.236 254 100 1 12 265 9 261 6.01e-112 323
MS.gene63307.t1 AT2G29350 61.961 255 96 1 11 265 11 264 6.88e-112 323
MS.gene63307.t1 AT2G29330 60.000 255 100 2 12 265 4 257 7.88e-108 313
MS.gene63307.t1 AT2G29370 56.391 266 115 1 1 265 1 266 1.17e-107 313
MS.gene63307.t1 AT2G30670 58.268 254 106 0 12 265 4 257 1.59e-107 312
MS.gene63307.t1 AT2G29340 57.087 254 109 0 12 265 4 257 1.64e-105 307
MS.gene63307.t1 AT2G29340 57.087 254 109 0 12 265 4 257 4.85e-105 307
MS.gene63307.t1 AT2G29310 57.874 254 107 0 12 265 4 257 1.07e-104 305
MS.gene63307.t1 AT2G29320 56.757 259 111 1 8 265 6 264 5.22e-103 301
MS.gene63307.t1 AT2G29290 59.829 234 94 0 32 265 7 240 1.98e-101 296
MS.gene63307.t1 AT2G29300 55.686 255 112 1 12 265 4 258 3.45e-100 293
MS.gene63307.t1 AT2G29300 55.200 250 111 1 12 260 4 253 2.44e-96 285
MS.gene63307.t1 AT2G29350 60.619 226 88 1 40 265 2 226 1.09e-95 281
MS.gene63307.t1 AT2G29310 57.021 235 101 0 31 265 21 255 5.42e-93 275
MS.gene63307.t1 AT2G29320 56.780 236 101 1 31 265 11 246 7.09e-93 275
MS.gene63307.t1 AT2G29350 62.887 194 72 0 11 204 11 204 5.33e-85 253
MS.gene63307.t1 AT2G29310 57.576 198 84 0 12 209 4 201 9.34e-78 234
MS.gene63307.t1 AT2G29340 55.556 198 88 0 12 209 4 201 1.24e-76 231
MS.gene63307.t1 AT2G29340 55.556 198 88 0 12 209 4 201 1.24e-76 231
MS.gene63307.t1 AT2G29320 55.838 197 86 1 8 203 6 202 9.50e-75 227
MS.gene63307.t1 AT1G07440 60.248 161 64 0 12 172 9 169 6.92e-69 211
MS.gene63307.t1 AT2G29310 56.425 179 78 0 31 209 21 199 1.01e-65 203
MS.gene63307.t1 AT2G29170 58.824 102 41 1 1 101 1 102 1.10e-34 121
MS.gene63307.t1 AT5G18210 33.962 265 156 9 8 259 1 259 9.10e-34 123
MS.gene63307.t1 AT4G05530 33.333 246 159 5 15 259 9 250 1.84e-33 122
MS.gene63307.t1 AT3G03980 33.829 269 159 9 6 259 5 269 3.06e-33 122
MS.gene63307.t1 AT4G13180 33.210 271 163 10 1 261 1 263 3.27e-31 117
MS.gene63307.t1 AT5G18210 33.824 272 147 11 8 266 1 252 2.00e-30 115
MS.gene63307.t1 AT3G04000 32.210 267 165 8 6 258 5 269 6.25e-27 105
MS.gene63307.t1 AT1G24360 30.364 247 159 8 21 261 80 319 7.09e-27 106
MS.gene63307.t1 AT3G55310 30.142 282 179 13 1 267 1 279 1.79e-25 102
MS.gene63307.t1 AT3G55290 30.451 266 169 11 15 267 17 279 1.08e-24 100
MS.gene63307.t1 AT3G55290 30.451 266 169 11 15 267 18 280 1.13e-24 100
MS.gene63307.t1 AT3G46170 29.845 258 170 8 15 263 26 281 3.01e-24 99.0
MS.gene63307.t1 AT3G51680 29.643 280 173 8 1 260 20 295 3.25e-24 99.4
MS.gene63307.t1 AT1G63380 29.078 282 183 10 1 267 1 280 3.73e-24 98.6
MS.gene63307.t1 AT1G63380 29.699 266 172 8 15 267 19 282 4.42e-24 98.6
MS.gene63307.t1 AT1G63380 29.699 266 172 8 15 267 15 278 4.75e-24 98.2
MS.gene63307.t1 AT1G63380 29.344 259 171 7 15 263 22 278 8.19e-24 97.8
MS.gene63307.t1 AT1G54870 32.707 266 150 10 15 263 84 337 1.60e-23 97.8
MS.gene63307.t1 AT1G54870 32.707 266 150 10 15 263 82 335 1.74e-23 97.8
MS.gene63307.t1 AT1G62610 28.571 266 175 8 15 267 14 277 2.36e-23 96.3
MS.gene63307.t1 AT1G62610 28.571 266 175 8 15 267 15 278 2.69e-23 96.3
MS.gene63307.t1 AT2G17845 29.389 262 171 7 15 264 47 306 3.07e-23 96.7
MS.gene63307.t1 AT1G62610 28.571 266 175 8 15 267 13 276 3.29e-23 95.9
MS.gene63307.t1 AT1G62610 28.571 266 175 8 15 267 19 282 3.47e-23 96.3
MS.gene63307.t1 AT3G26760 29.502 261 163 8 15 260 36 290 7.84e-23 95.5
MS.gene63307.t1 AT1G52340 31.061 264 158 8 15 260 18 275 2.30e-22 94.0
MS.gene63307.t1 AT2G47130 30.651 261 166 9 8 261 1 253 2.76e-22 93.2
MS.gene63307.t1 AT3G05260 33.205 259 155 9 15 262 37 288 9.77e-21 89.7
MS.gene63307.t1 AT2G47120 29.008 262 168 11 8 261 1 252 1.49e-20 88.6
MS.gene63307.t1 AT2G47120 28.627 255 166 10 15 261 7 253 4.28e-20 87.4
MS.gene63307.t1 AT3G12800 25.391 256 177 5 15 259 10 262 5.22e-20 87.8
MS.gene63307.t1 AT4G03140 26.236 263 166 6 15 260 78 329 4.91e-19 85.5
MS.gene63307.t1 AT3G42960 29.730 259 158 9 18 260 11 261 5.76e-19 84.3
MS.gene63307.t1 AT3G29260 29.389 262 168 9 8 261 1 253 2.27e-17 79.7
MS.gene63307.t1 AT3G26770 26.966 267 174 7 15 267 41 300 2.40e-17 80.5
MS.gene63307.t1 AT3G29250 28.897 263 173 8 7 261 36 292 5.00e-17 79.3
MS.gene63307.t1 AT2G47140 28.897 263 169 11 8 261 1 254 9.00e-17 78.2
MS.gene63307.t1 AT3G29250 29.008 262 170 9 8 261 1 254 7.35e-16 75.5
MS.gene63307.t1 AT3G29250 28.897 263 171 9 7 261 36 290 9.26e-16 75.9
MS.gene63307.t1 AT4G13250 27.413 259 149 8 18 243 162 414 2.11e-15 75.9
MS.gene63307.t1 AT5G04900 29.464 224 136 9 21 229 83 299 2.54e-15 75.1
MS.gene63307.t1 AT1G10310 32.143 168 111 3 18 184 18 183 1.46e-13 68.9
MS.gene63307.t1 AT4G13250 27.027 259 148 9 18 243 162 412 3.18e-13 69.7
MS.gene63307.t1 AT3G59710 28.333 240 120 6 12 204 13 247 4.46e-13 68.2
MS.gene63307.t1 AT3G59710 28.333 240 120 6 12 204 42 276 8.08e-13 67.8
MS.gene63307.t1 AT1G24470 27.500 200 136 6 17 210 52 248 8.63e-13 67.4
MS.gene63307.t1 AT3G03330 28.497 193 129 5 12 197 38 228 2.66e-12 66.2
MS.gene63307.t1 AT5G65205 28.889 180 117 6 21 197 12 183 4.20e-12 65.5
MS.gene63307.t1 AT5G10050 26.702 191 133 3 21 210 11 195 4.80e-12 65.1
MS.gene63307.t1 AT4G09750 33.544 158 86 5 15 167 41 184 5.60e-12 65.5
MS.gene63307.t1 AT1G49670 28.972 214 125 7 17 211 6 211 6.05e-12 65.9
MS.gene63307.t1 AT1G49670 28.972 214 125 7 17 211 6 211 6.20e-12 65.9
MS.gene63307.t1 AT3G06060 26.733 202 141 2 9 207 31 228 8.19e-12 64.7
MS.gene63307.t1 AT3G06060 26.733 202 141 2 9 207 48 245 8.39e-12 65.1
MS.gene63307.t1 AT3G47360 29.688 192 124 6 18 204 48 233 5.49e-11 62.4

Find 47 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTCTTCTTAAAGAATC+TGG 0.311673 3.3:-60328784 MS.gene63307:CDS
GTTTCTGCTATACGCTTTAT+CGG 0.318496 3.3:+60328016 None:intergenic
AATGGGCAAAAGACAATATA+AGG 0.326266 3.3:-60328547 MS.gene63307:CDS
TGCCTCTCATGCTGCTCTCT+TGG 0.339487 3.3:+60329981 None:intergenic
GGAAAGTCTGCTCTAGTTAC+TGG 0.355585 3.3:-60329941 MS.gene63307:CDS
GGACAAACTGTTTCTGTTGA+TGG 0.363679 3.3:-60327935 MS.gene63307:CDS
CTTCTTAAAGAATCTGGAAA+TGG 0.409640 3.3:-60328778 MS.gene63307:CDS
TCGGCTGTATACTCAATTGT+TGG 0.433889 3.3:+60328868 None:intergenic
ACTCACTGCATAGATGCTTC+CGG 0.439791 3.3:+60328708 None:intergenic
TGCATTCCATCTATGTCAAC+TGG 0.450264 3.3:-60328809 MS.gene63307:CDS
GATGGAGGATTTACTGTGAA+TGG 0.451398 3.3:-60327917 MS.gene63307:CDS
AAAAGTGATGACTACTAACT+TGG 0.463013 3.3:-60328836 MS.gene63307:CDS
ACTGGTGGAACTCGTGGAAT+CGG 0.476543 3.3:-60329923 MS.gene63307:CDS
GTGGAGGAACTAGCTGAGTT+TGG 0.479590 3.3:-60329890 MS.gene63307:CDS
TTTCCAGATTCTTTAAGAAG+AGG 0.503369 3.3:+60328781 None:intergenic
AACCAAGAGAGCAGCATGAG+AGG 0.505029 3.3:-60329983 MS.gene63307:CDS
CCAGCTGCTTCCTACATCAC+TGG 0.520639 3.3:-60327956 MS.gene63307:CDS
CAAGTTGCTTCTGCTTTCAA+CGG 0.524543 3.3:-60329731 MS.gene63307:CDS
CTTAAATGAGTGGAAAGAGA+AGG 0.530669 3.3:-60329819 MS.gene63307:CDS
AGCATCTATGCAGTGAGTAA+AGG 0.540433 3.3:-60328703 MS.gene63307:intron
CTAGTTACTGGTGGAACTCG+TGG 0.541240 3.3:-60329929 MS.gene63307:CDS
AGCTATTAGGTAAACAATGC+TGG 0.541729 3.3:-60328907 MS.gene63307:intron
TTAAATGAGTGGAAAGAGAA+GGG 0.543808 3.3:-60329818 MS.gene63307:CDS
AACAGTTTGTCCAGTGATGT+AGG 0.560729 3.3:+60327946 None:intergenic
AGGCACAAGATGGTCTCTCA+AGG 0.570614 3.3:-60329963 MS.gene63307:CDS
GATGTGCCAGTTGACATAGA+TGG 0.576163 3.3:+60328803 None:intergenic
CCACAGTGTACACTTGCTCT+AGG 0.579593 3.3:-60329865 MS.gene63307:CDS
AATCGGTCATGCTGTGGTGG+AGG 0.580717 3.3:-60329906 MS.gene63307:CDS
ATAAGCTCCTCTCTTTGAGA+AGG 0.588037 3.3:+60329758 None:intergenic
CCAGTGATGTAGGAAGCAGC+TGG 0.588298 3.3:+60327956 None:intergenic
ATCTTCTCCTTCTCAAAGAG+AGG 0.595566 3.3:-60329765 MS.gene63307:CDS
GCAGCATGAGAGGCACAAGA+TGG 0.597950 3.3:-60329973 MS.gene63307:CDS
TCTCTGATAAGCGTAGGTAC+CGG 0.606028 3.3:-60328727 MS.gene63307:CDS
AACACATGAAGTGTCTGCCT+TGG 0.606041 3.3:-60327996 MS.gene63307:CDS
TGGAATCGGTCATGCTGTGG+TGG 0.606109 3.3:-60329909 MS.gene63307:CDS
CAAACTGTTTCTGTTGATGG+AGG 0.607479 3.3:-60327932 MS.gene63307:CDS
AAGTCTGCTCTAGTTACTGG+TGG 0.609393 3.3:-60329938 MS.gene63307:CDS
AGGAAGCAGCTGGCAGACAA+AGG 0.611292 3.3:+60327966 None:intergenic
TGAGCAAATGCTTAAATGAG+TGG 0.614861 3.3:-60329829 MS.gene63307:CDS
GTTGCATCTCTGATAAGCGT+AGG 0.625038 3.3:-60328733 MS.gene63307:CDS
TCGTGGAATCGGTCATGCTG+TGG 0.634799 3.3:-60329912 MS.gene63307:CDS
GGCACAAGATGGTCTCTCAA+GGG 0.644640 3.3:-60329962 MS.gene63307:CDS
ACAATGCTGGTACAAATGTG+AGG 0.662281 3.3:-60328894 MS.gene63307:CDS
GCTTATCAGAGATGCAACAG+AGG 0.678018 3.3:+60328738 None:intergenic
GGTGCAACTGTGTTGCACCC+TGG 0.678077 3.3:-60328526 MS.gene63307:CDS
CAGACAAAGGAAAGTCACCA+AGG 0.716868 3.3:+60327979 None:intergenic
CCTAGAGCAAGTGTACACTG+TGG 0.805980 3.3:+60329865 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGTAGAAGTTATAGA+AGG - chr3.3:60328647-60328666 MS.gene63307:intron 20.0%
!! GAAAACGAAATATATAGAAA+AGG + chr3.3:60328205-60328224 None:intergenic 20.0%
!! TTCATAAAGAAAAAATGACA+AGG + chr3.3:60329450-60329469 None:intergenic 20.0%
!!! TACTATTTTATTTTTTCACC+AGG + chr3.3:60328636-60328655 None:intergenic 20.0%
! ACATCAACATGATATAGAAA+CGG + chr3.3:60328227-60328246 None:intergenic 25.0%
! ATCTGTAGTATTAATCCAAA+TGG - chr3.3:60329751-60329770 MS.gene63307:CDS 25.0%
! ATCTTTAATTATCCATTCTC+TGG - chr3.3:60329424-60329443 MS.gene63307:intron 25.0%
! CTAATGCATTATAACTCTAA+CGG - chr3.3:60328563-60328582 MS.gene63307:CDS 25.0%
! TGTGCTAGTTGAAAATAATT+TGG + chr3.3:60328395-60328414 None:intergenic 25.0%
!! ATGTTTTATGATTTTCTTCC+TGG - chr3.3:60328615-60328634 MS.gene63307:intron 25.0%
!! CTTGTTATAAGACTTTCTTA+TGG - chr3.3:60328853-60328872 MS.gene63307:CDS 25.0%
!! TGATTTGTATGTTTGTCTTA+AGG - chr3.3:60328437-60328456 MS.gene63307:intron 25.0%
!!! CATCACTTTTGAATAATCTT+CGG + chr3.3:60329038-60329057 None:intergenic 25.0%
AAAAAGTCTTGCTTGTGAAT+GGG - chr3.3:60329320-60329339 MS.gene63307:intron 30.0%
AAAAGTGATGACTACTAACT+TGG - chr3.3:60329048-60329067 MS.gene63307:intron 30.0%
AACCGAAATCATGTATTAGT+AGG - chr3.3:60329609-60329628 MS.gene63307:intron 30.0%
AATGGGCAAAAGACAATATA+AGG - chr3.3:60329337-60329356 MS.gene63307:intron 30.0%
ACTATGCTAAGGTTGTTATA+AGG - chr3.3:60328710-60328729 MS.gene63307:CDS 30.0%
AGCCTACTAATACATGATTT+CGG + chr3.3:60329614-60329633 None:intergenic 30.0%
CAGCAAAAATTAAAATGCAG+AGG + chr3.3:60329693-60329712 None:intergenic 30.0%
CTTCTTAAAGAATCTGGAAA+TGG - chr3.3:60329106-60329125 MS.gene63307:intron 30.0%
TGATAATCTTGTGAGCTATT+AGG - chr3.3:60328964-60328983 MS.gene63307:intron 30.0%
TGTTATGTTCAGAACTTATG+TGG - chr3.3:60329777-60329796 MS.gene63307:CDS 30.0%
TTAAATGAGTGGAAAGAGAA+GGG - chr3.3:60328066-60328085 MS.gene63307:intron 30.0%
TTAGCAATAAACTGCAAGAA+AGG + chr3.3:60329814-60329833 None:intergenic 30.0%
! TGCATTCTTTTGATGTACTT+TGG - chr3.3:60328830-60328849 MS.gene63307:CDS 30.0%
! TTTCCAGATTCTTTAAGAAG+AGG + chr3.3:60329106-60329125 None:intergenic 30.0%
!! TTACTGTGAATGGATTTTCT+GGG - chr3.3:60329977-60329996 MS.gene63307:CDS 30.0%
!! TTTACTGTGAATGGATTTTC+TGG - chr3.3:60329976-60329995 MS.gene63307:CDS 30.0%
!!! GAGAAGGGTTTTTTTGTTTA+TGG - chr3.3:60328081-60328100 MS.gene63307:intron 30.0%
!!! GGGTTTTTTTGTTTATGGAT+CGG - chr3.3:60328086-60328105 MS.gene63307:intron 30.0%
AAATCAAGTGCGTGTAAGAT+TGG + chr3.3:60328778-60328797 None:intergenic 35.0%
AATCAATTTGTAGATGCTCG+TGG - chr3.3:60328332-60328351 MS.gene63307:intron 35.0%
AGCTATTAGGTAAACAATGC+TGG - chr3.3:60328977-60328996 MS.gene63307:intron 35.0%
CAAAAAGTCTTGCTTGTGAA+TGG - chr3.3:60329319-60329338 MS.gene63307:intron 35.0%
CATCCTCTTCTTAAAGAATC+TGG - chr3.3:60329100-60329119 MS.gene63307:intron 35.0%
CTGAACATAACATTGCCATT+TGG + chr3.3:60329769-60329788 None:intergenic 35.0%
CTTAAATGAGTGGAAAGAGA+AGG - chr3.3:60328065-60328084 MS.gene63307:intron 35.0%
GTATTATTGAATCGCAACGT+AGG + chr3.3:60329504-60329523 None:intergenic 35.0%
GTTTCTGCTATACGCTTTAT+CGG + chr3.3:60329871-60329890 None:intergenic 35.0%
TACCAGAAACAAGACGAAAA+AGG + chr3.3:60328907-60328926 None:intergenic 35.0%
TGAGCAAATGCTTAAATGAG+TGG - chr3.3:60328055-60328074 MS.gene63307:CDS 35.0%
! GATGTTGAATTTTGCATAGC+TGG - chr3.3:60328248-60328267 MS.gene63307:intron 35.0%
!! TAAAATGCAGAGGCATCATA+TGG + chr3.3:60329683-60329702 None:intergenic 35.0%
!!! AGTTCACTTTTTGAGTGTGT+TGG - chr3.3:60328532-60328551 MS.gene63307:CDS 35.0%
AAAATGACAAGGCCAGAGAA+TGG + chr3.3:60329439-60329458 None:intergenic 40.0%
AACAGTTTGTCCAGTGATGT+AGG + chr3.3:60329941-60329960 None:intergenic 40.0%
ACAATGCTGGTACAAATGTG+AGG - chr3.3:60328990-60329009 MS.gene63307:intron 40.0%
AGCATCTATGCAGTGAGTAA+AGG - chr3.3:60329181-60329200 MS.gene63307:intron 40.0%
ATCTTCTCCTTCTCAAAGAG+AGG - chr3.3:60328119-60328138 MS.gene63307:intron 40.0%
ATGCATACGAAACACGATCA+TGG - chr3.3:60329550-60329569 MS.gene63307:intron 40.0%
ATGCTCGTGGAAAACTTAGT+TGG - chr3.3:60328345-60328364 MS.gene63307:intron 40.0%
CAAGTTGCTTCTGCTTTCAA+CGG - chr3.3:60328153-60328172 MS.gene63307:intron 40.0%
GATGGAGGATTTACTGTGAA+TGG - chr3.3:60329967-60329986 MS.gene63307:CDS 40.0%
GTATACAAAAACGTCGCTTG+TGG - chr3.3:60329380-60329399 MS.gene63307:intron 40.0%
TCGGCTGTATACTCAATTGT+TGG + chr3.3:60329019-60329038 None:intergenic 40.0%
TGATTTCGGTTGCATGAATC+AGG + chr3.3:60329600-60329619 None:intergenic 40.0%
TGCAGTGAGTAAAGGTAACT+TGG - chr3.3:60329189-60329208 MS.gene63307:intron 40.0%
TGCATACGAAACACGATCAT+GGG - chr3.3:60329551-60329570 MS.gene63307:intron 40.0%
TGCATTCCATCTATGTCAAC+TGG - chr3.3:60329075-60329094 MS.gene63307:intron 40.0%
! AAGCGACGTTTTTGTATACC+AGG + chr3.3:60329379-60329398 None:intergenic 40.0%
! AGCGACGTTTTTGTATACCA+GGG + chr3.3:60329378-60329397 None:intergenic 40.0%
! ATAAGCTCCTCTCTTTGAGA+AGG + chr3.3:60328129-60328148 None:intergenic 40.0%
!! ACACGCACTTGATTTTCAAG+TGG - chr3.3:60328783-60328802 MS.gene63307:CDS 40.0%
!! CAAACTGTTTCTGTTGATGG+AGG - chr3.3:60329952-60329971 MS.gene63307:CDS 40.0%
!! GGACAAACTGTTTCTGTTGA+TGG - chr3.3:60329949-60329968 MS.gene63307:CDS 40.0%
!!! CACCTTTTTCGTCTTGTTTC+TGG - chr3.3:60328902-60328921 MS.gene63307:intron 40.0%
AAAAACGTCGCTTGTGGAAC+AGG - chr3.3:60329386-60329405 MS.gene63307:intron 45.0%
AAGTCTGCTCTAGTTACTGG+TGG - chr3.3:60327946-60327965 MS.gene63307:CDS 45.0%
ACTCACTGCATAGATGCTTC+CGG + chr3.3:60329179-60329198 None:intergenic 45.0%
AGATGCTCACACTGACACTA+CGG + chr3.3:60329735-60329754 None:intergenic 45.0%
CAGACAAAGGAAAGTCACCA+AGG + chr3.3:60329908-60329927 None:intergenic 45.0%
GAGAGCTTGTCACTATGCTA+AGG - chr3.3:60328699-60328718 MS.gene63307:intron 45.0%
GATGTGCCAGTTGACATAGA+TGG + chr3.3:60329084-60329103 None:intergenic 45.0%
GCTTATCAGAGATGCAACAG+AGG + chr3.3:60329149-60329168 None:intergenic 45.0%
GGAAAGTCTGCTCTAGTTAC+TGG - chr3.3:60327943-60327962 MS.gene63307:CDS 45.0%
GTTGCATCTCTGATAAGCGT+AGG - chr3.3:60329151-60329170 MS.gene63307:intron 45.0%
TCTCTGATAAGCGTAGGTAC+CGG - chr3.3:60329157-60329176 MS.gene63307:intron 45.0%
! AACACATGAAGTGTCTGCCT+TGG - chr3.3:60329888-60329907 MS.gene63307:CDS 45.0%
!! AATGCAGAGGCATCATATGG+TGG + chr3.3:60329680-60329699 None:intergenic 45.0%
AACCAAGAGAGCAGCATGAG+AGG - chr3.3:60327901-60327920 MS.gene63307:CDS 50.0%
ACTGGTGGAACTCGTGGAAT+CGG - chr3.3:60327961-60327980 MS.gene63307:CDS 50.0%
AGGCACAAGATGGTCTCTCA+AGG - chr3.3:60327921-60327940 MS.gene63307:CDS 50.0%
AGTGCGTGTAAGATTGGCGA+CGG + chr3.3:60328772-60328791 None:intergenic 50.0%
CCACAGTGTACACTTGCTCT+AGG - chr3.3:60328019-60328038 MS.gene63307:CDS 50.0%
CCTAGAGCAAGTGTACACTG+TGG + chr3.3:60328022-60328041 None:intergenic 50.0%
CTAGTTACTGGTGGAACTCG+TGG - chr3.3:60327955-60327974 MS.gene63307:CDS 50.0%
GGCACAAGATGGTCTCTCAA+GGG - chr3.3:60327922-60327941 MS.gene63307:CDS 50.0%
GTGGAGGAACTAGCTGAGTT+TGG - chr3.3:60327994-60328013 MS.gene63307:CDS 50.0%
TCACTGTGTGTGTGTGTGTG+AGG + chr3.3:60328681-60328700 None:intergenic 50.0%
AATCGGTCATGCTGTGGTGG+AGG - chr3.3:60327978-60327997 MS.gene63307:CDS 55.0%
AGGAAGCAGCTGGCAGACAA+AGG + chr3.3:60329921-60329940 None:intergenic 55.0%
CCAGCTGCTTCCTACATCAC+TGG - chr3.3:60329928-60329947 MS.gene63307:CDS 55.0%
CCAGTGATGTAGGAAGCAGC+TGG + chr3.3:60329931-60329950 None:intergenic 55.0%
CTGCTTACTCCACCTAGCGA+GGG - chr3.3:60328733-60328752 MS.gene63307:CDS 55.0%
GAAACACGATCATGGGCTCG+TGG - chr3.3:60329558-60329577 MS.gene63307:intron 55.0%
TCGTGGAATCGGTCATGCTG+TGG - chr3.3:60327972-60327991 MS.gene63307:CDS 55.0%
TGCCTCTCATGCTGCTCTCT+TGG + chr3.3:60327906-60327925 None:intergenic 55.0%
TGGAATCGGTCATGCTGTGG+TGG - chr3.3:60327975-60327994 MS.gene63307:CDS 55.0%
! GCAGCATGAGAGGCACAAGA+TGG - chr3.3:60327911-60327930 MS.gene63307:CDS 55.0%
GCTGCTTACTCCACCTAGCG+AGG - chr3.3:60328732-60328751 MS.gene63307:CDS 60.0%
GGTGCAACTGTGTTGCACCC+TGG - chr3.3:60329358-60329377 MS.gene63307:intron 60.0%
TCATGGGCTCGTGGCTCATC+TGG - chr3.3:60329567-60329586 MS.gene63307:intron 60.0%
! CTAGCGAGGGCCTTGTGCAT+GGG - chr3.3:60328746-60328765 MS.gene63307:CDS 60.0%
CCATGCACAAGGCCCTCGCT+AGG + chr3.3:60328748-60328767 None:intergenic 65.0%
TGCACAAGGCCCTCGCTAGG+TGG + chr3.3:60328745-60328764 None:intergenic 65.0%
TTGGCGACGGCCCATGCACA+AGG + chr3.3:60328759-60328778 None:intergenic 65.0%
! CCTAGCGAGGGCCTTGTGCA+TGG - chr3.3:60328745-60328764 MS.gene63307:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 60327895 60330011 60327895 ID=MS.gene63307
chr3.3 mRNA 60327895 60330011 60327895 ID=MS.gene63307.t1;Parent=MS.gene63307
chr3.3 exon 60329715 60330011 60329715 ID=MS.gene63307.t1.exon1;Parent=MS.gene63307.t1
chr3.3 CDS 60329715 60330011 60329715 ID=cds.MS.gene63307.t1;Parent=MS.gene63307.t1
chr3.3 exon 60328704 60328920 60328704 ID=MS.gene63307.t1.exon2;Parent=MS.gene63307.t1
chr3.3 CDS 60328704 60328920 60328704 ID=cds.MS.gene63307.t1;Parent=MS.gene63307.t1
chr3.3 exon 60328499 60328605 60328499 ID=MS.gene63307.t1.exon3;Parent=MS.gene63307.t1
chr3.3 CDS 60328499 60328605 60328499 ID=cds.MS.gene63307.t1;Parent=MS.gene63307.t1
chr3.3 exon 60327895 60328083 60327895 ID=MS.gene63307.t1.exon4;Parent=MS.gene63307.t1
chr3.3 CDS 60327895 60328083 60327895 ID=cds.MS.gene63307.t1;Parent=MS.gene63307.t1
Gene Sequence

>MS.gene63307

ATGGAGAACCAAGAGAGCAGCATGAGAGGCACAAGATGGTCTCTCAAGGGAAAGTCTGCTCTAGTTACTGGTGGAACTCGTGGAATCGGTCATGCTGTGGTGGAGGAACTAGCTGAGTTTGGTGCCACAGTGTACACTTGCTCTAGGAATGAAGAAGAGCTGAGCAAATGCTTAAATGAGTGGAAAGAGAAGGGTTTTTTTGTTTATGGATCGGTTTGTGATGCATCTTCTCCTTCTCAAAGAGAGGAGCTTATTCGACAAGTTGCTTCTGCTTTCAACGGCAAGCTCAACATACTTGTGAGTTTTTCCTTTTCTATATATTTCGTTTTCCGTTTCTATATCATGTTGATGTTGATGTTGAATTTTGCATAGCTGGTAGTCTTTTAATTCATGAAAATTACTCGTACAATACAAATATTCAGTTTTTAACTTCTTTTAATCAATTTGTAGATGCTCGTGGAAAACTTAGTTGGAAAAGTTTTTGAAAAATTGAAATGCCAAATTATTTTCAACTAGCACAACAGAATATACTATTTACAATTTGATTTGTATGTTTGTCTTAAGGTTATATTAATGTTTACTTTCTATAAGAATTTGTATTGTATTTATCAAACTTACTCTTATAGTCTCGTTCATCAGTTCACTTTTTGAGTGTGTTGGAGATTGATCTAATGCATTATAACTCTAACGGTGTTTCATCGTTCTGTGTATTGCTGCAACATGTTTTATGATTTTCTTCCTGGTGAAAAAATAAAATAGTAGAAGTTATAGAAGGTTGAAATGCCTCACACACACACACACAGTGAGAGCTTGTCACTATGCTAAGGTTGTTATAAGGCTGCTTACTCCACCTAGCGAGGGCCTTGTGCATGGGCCGTCGCCAATCTTACACGCACTTGATTTTCAAGTGGTTACATTACATACTTAAAAAAAATTGCATTCTTTTGATGTACTTTGGCTTGTTATAAGACTTTCTTATGGATGCTTCAAATACACTCAGAAATGTTCACCTTTTTCGTCTTGTTTCTGGTATGCATGACTTTAACTTATAATAAAAGTTATGTTCATATGATAATCTTGTGAGCTATTAGGTAAACAATGCTGGTACAAATGTGAGGAAGCCAACAATTGAGTATACAGCCGAAGATTATTCAAAAGTGATGACTACTAACTTGGATTCTGCATTCCATCTATGTCAACTGGCACATCCTCTTCTTAAAGAATCTGGAAATGGAAGCATTGTATTCAATTCCTCTGTTGCATCTCTGATAAGCGTAGGTACCGGAAGCATCTATGCAGTGAGTAAAGGTAACTTGGTGATCATCATGTCTACTTAATTTGCATAATGAGTACAGACTATTTTGATAGACATTTACTGAAGCGATTCACTGTGTTGTTTTCTTCAGCTGCAATCAATCAGCTGACAAAAAGTCTTGCTTGTGAATGGGCAAAAGACAATATAAGGTGCAACTGTGTTGCACCCTGGTATACAAAAACGTCGCTTGTGGAACAGGTAATGCTCTTTTCACATCTTTAATTATCCATTCTCTGGCCTTGTCATTTTTTCTTTATGAAGATTGCTTAAGTTTGTAAATATATATTTTCTCCTACGTTGCGATTCAATAATACTCTTATCATGTTACTCTGATTCACGTATGCATACGAAACACGATCATGGGCTCGTGGCTCATCTGGATGTTAGCCTGATTCATGCAACCGAAATCATGTATTAGTAGGCTAACTCTTTAAGTGTTGATCAATGAAAAACATATCTGTAACTATCCACCATATGATGCCTCTGCATTTTAATTTTTGCTGAAAATAAACATATGTGTAACCGTAGTGTCAGTGTGAGCATCTGTAGTATTAATCCAAATGGCAATGTTATGTTCAGAACTTATGTGGCTAATAATTTACCTTTCTTGCAGTTTATTGCTAACAAAGAGTTTGTGAATGAAGTATTATCTCGAACACCGATAAAGCGTATAGCAGAAACACATGAAGTGTCTGCCTTGGTGACTTTCCTTTGTCTGCCAGCTGCTTCCTACATCACTGGACAAACTGTTTCTGTTGATGGAGGATTTACTGTGAATGGATTTTCTGGGTTGTTTAAATAA
Protein sequence

>MS.gene63307.t1

MENQESSMRGTRWSLKGKSALVTGGTRGIGHAVVEELAEFGATVYTCSRNEEELSKCLNEWKEKGFFVYGSVCDASSPSQREELIRQVASAFNGKLNILVNNAGTNVRKPTIEYTAEDYSKVMTTNLDSAFHLCQLAHPLLKESGNGSIVFNSSVASLISVGTGSIYAVSKAAINQLTKSLACEWAKDNIRCNCVAPWYTKTSLVEQFIANKEFVNEVLSRTPIKRIAETHEVSALVTFLCLPAASYITGQTVSVDGGFTVNGFSGLFK