AlfalfaGEDB | MS.gene63300

M. sativa cultivar XinJiangDaYe / MS.gene63300

KEGG: K08081
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63300.t1	XP_013460715.1	96.7	275	9	0	1	275	1	275	1.10E-146	529.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63300.t1	Q9LHT0	65.6	259	89	0	15	273	5	263	4.7e-98	359.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63300.t1	A0A072UY64	96.7	275	9	0	1	275	1	275	7.9e-147	529.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene003925	MS.gene63300	PPI
MS.gene003926	MS.gene63300	PPI
MS.gene006999	MS.gene63300	PPI
MS.gene007006	MS.gene63300	PPI
MS.gene007678	MS.gene63300	PPI
MS.gene012681	MS.gene63300	PPI
MS.gene012794	MS.gene63300	PPI
MS.gene014425	MS.gene63300	PPI
MS.gene018066	MS.gene63300	PPI
MS.gene019786	MS.gene63300	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63300.t1	MTR_3g068155	96.727	275	9	0	1	275	1	275	0.0	551
MS.gene63300.t1	MTR_3g068175	78.676	272	58	0	4	275	2	273	1.10e-158	442
MS.gene63300.t1	MTR_3g068175	77.536	276	58	1	4	275	2	277	3.84e-156	436
MS.gene63300.t1	MTR_3g068085	77.432	257	58	0	19	275	15	271	1.07e-151	424
MS.gene63300.t1	MTR_3g068070	75.769	260	63	0	9	268	5	264	1.82e-149	419
MS.gene63300.t1	MTR_3g068065	73.438	256	68	0	19	274	17	272	1.21e-142	402
MS.gene63300.t1	MTR_4g064883	64.591	257	91	0	15	271	10	266	1.85e-129	368
MS.gene63300.t1	MTR_4g064887	61.776	259	99	0	7	265	3	261	2.42e-121	347
MS.gene63300.t1	MTR_7g063320	58.175	263	106	1	7	265	3	265	4.15e-110	319
MS.gene63300.t1	MTR_7g063390	58.846	260	103	1	10	265	7	266	1.68e-106	310

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63300.t1	AT5G06060	65.637	259	89	0	15	273	5	263	1.59e-132	375
MS.gene63300.t1	AT2G29260	61.719	256	98	0	15	270	64	319	2.82e-120	347
MS.gene63300.t1	AT2G29290	62.403	258	95	1	15	270	3	260	3.80e-119	342
MS.gene63300.t1	AT1G07440	61.240	258	97	2	15	270	8	264	1.89e-118	340
MS.gene63300.t1	AT1G07450	62.598	254	94	1	15	268	4	256	7.58e-118	338
MS.gene63300.t1	AT2G29150	60.227	264	102	2	9	270	6	268	5.78e-116	334
MS.gene63300.t1	AT2G29360	60.385	260	103	0	9	268	6	265	7.67e-115	331
MS.gene63300.t1	AT2G29350	61.660	253	96	1	16	268	12	263	2.67e-113	327
MS.gene63300.t1	AT2G29340	57.308	260	109	1	15	272	3	262	1.03e-111	323
MS.gene63300.t1	AT2G29340	57.752	258	107	1	15	270	3	260	6.04e-111	323

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Find 60 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTACATGTTCTACAAG+TGG	0.573526	3.3:-60407454	None:intergenic
AAATTACATGTTCTACAAGT+GGG	0.546415	3.3:-60407453	None:intergenic
AAGATGCAGCAGGTAAGCAA+AGG	0.634488	3.3:-60408113	None:intergenic
AATGGGCAATAGACAACATA+AGG	0.496335	3.3:+60407398	MS.gene63300:CDS
AATTACATGTTCTACAAGTG+GGG	0.636533	3.3:-60407452	None:intergenic
ACAAGTTATTTGTGTTGATG+GGG	0.632558	3.3:+60408146	MS.gene63300:CDS
ACCAAAGAGGAGACCTCTTC+GGG	0.477426	3.3:-60408084	None:intergenic
ACCCGAAGAGGTCTCCTCTT+TGG	0.381912	3.3:+60408083	MS.gene63300:CDS
ACCGGTGGCACTCGTGGAAT+CGG	0.347184	3.3:+60405689	MS.gene63300:CDS
AGAGAGCCTTATTCAACAAG+TGG	0.562362	3.3:+60406196	MS.gene63300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAATTTGGCCTGTGAAT+GGG	+	chr3.3:60407381-60407400	MS.gene63300:CDS	30.0%
AAACGACGCTAATCTTAAAA+CGG	-	chr3.3:60405826-60405845	None:intergenic	30.0%
AATTACATGTTCTACAAGTG+GGG	-	chr3.3:60407455-60407474	None:intergenic	30.0%
AATTGCAGCTGAAAATTCAA+TGG	-	chr3.3:60407350-60407369	None:intergenic	30.0%
ACCAACACTACAACATAATT+GGG	+	chr3.3:60405864-60405883	MS.gene63300:intron	30.0%
ACCGTTTATAAACTCTCTTA+AGG	-	chr3.3:60406581-60406600	None:intergenic	30.0%
ATTCATTTGACACATTCTCT+AGG	+	chr3.3:60406021-60406040	MS.gene63300:intron	30.0%
CAACAAAAATCAGGAAGAAA+CGG	-	chr3.3:60406816-60406835	None:intergenic	30.0%
CCACATAGAACCAATAAATA+TGG	+	chr3.3:60407539-60407558	MS.gene63300:intron	30.0%
CTATTGGACTAAGTTTGAAT+TGG	+	chr3.3:60406337-60406356	MS.gene63300:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	60405611	60408212	60405611	ID=MS.gene63300
chr3.3	mRNA	60405611	60408212	60405611	ID=MS.gene63300.t1;Parent=MS.gene63300
chr3.3	exon	60405611	60405711	60405611	ID=MS.gene63300.t1.exon1;Parent=MS.gene63300.t1
chr3.3	CDS	60405611	60405711	60405611	ID=cds.MS.gene63300.t1;Parent=MS.gene63300.t1
chr3.3	exon	60406043	60406250	60406043	ID=MS.gene63300.t1.exon2;Parent=MS.gene63300.t1
chr3.3	CDS	60406043	60406250	60406043	ID=cds.MS.gene63300.t1;Parent=MS.gene63300.t1
chr3.3	exon	60407043	60407259	60407043	ID=MS.gene63300.t1.exon3;Parent=MS.gene63300.t1
chr3.3	CDS	60407043	60407259	60407043	ID=cds.MS.gene63300.t1;Parent=MS.gene63300.t1
chr3.3	exon	60407362	60407468	60407362	ID=MS.gene63300.t1.exon4;Parent=MS.gene63300.t1
chr3.3	CDS	60407362	60407468	60407362	ID=cds.MS.gene63300.t1;Parent=MS.gene63300.t1
chr3.3	exon	60408018	60408212	60408018	ID=MS.gene63300.t1.exon5;Parent=MS.gene63300.t1
chr3.3	CDS	60408018	60408212	60408018	ID=cds.MS.gene63300.t1;Parent=MS.gene63300.t1

Gene Sequence

>MS.gene63300

ATGGCAGCAGCAGCAGTAGAAACACATAGCAGCAGAGGTGGACAGAGATGGTCTCTCACTGGAATGACAGCTCTTGTAACCGGTGGCACTCGTGGAATCGGGTTTTTATCACTCCCCTTCTCTCTTATTTCATTATTTATCTTATCTTCAACACCCTGCATCATTATTTTATTTTGATCTAGCCAAAAATACTTCCTTATCTTTTACTCCCTCCGTTTTAAGATTAGCGTCGTTTAAATGTTTTTACACACGGACCAACACTACAACATAATTGGGATCTAGTTACATTCGAACAATTGTTAAGTAAAATCCGTGTTACTAAAGAGCTCTACCAACATTTTATAGTATTATCTATCAGTAAGTTATTCAAAGAGATGACACTTTTATTAAGAATAGATTTTAATCCATCCATTCATTTGACACATTCTCTAGGCATGCCATAGTGAATGATCTAGTTGCATTTGGTGCTACTGTACACACTTGTTCAAGGACTGAATCAGAGCTCAATAAGTGCTTACAAGAATGGCAAAGTAAAGGATTTTCGGTTACTGGATCGGTCTGTGATGTAAGCTCTCGGCCACAAAGAGAGAGCCTTATTCAACAAGTGGCTTCCACTTTCAATGGCAAGCTCAACATCTTTGTAAGTACTCATTGTGCCAAACATATAAATCATTAATAAGAAAATATGCATTGTTGACTGTTCTATTCATGGATCTATGCAGAAAACTATTGGACTAAGTTTGAATTGGCATAGGTTTGTTGATAGTAAAGGCTTGAATTGAGTAGTAGTGTATGTTGTACTTTGAATTCAACTTGATCCATTAACTTAATTATAAAGAGTGATGACAATGGGAAAGTGGATAATATCTATGCTGCAACACTGTAAACACTTCTCCCATAAGTCCATAACAAACTTGAGTTGTTGCTATTGGAAAAAACGTAAGTTCCACATTGAAAAAAGATAACACCTTAAGAGAGTTTATAAACGGTAATATCCTTTATCTTCAAAATTATCCAAACATCAAGTCAAATAGTGAAAAGAGTTTGTAAATACTAAACAGTGACATATCTTACCTTACAAATAATTACTATTTTACTTCATCTGTTATCTTACATTTTATCCTTTTTATGTAATCTGCTTAAGATTTTTGTTTAAAAATTATACTAGTTGATCAATGTTTAGAGTTGAGGTCAACAACAAACCGTTTCTTCCTGATTTTTGTTGATTCCTGAACTCATCATTTGGATCTAGTTATTTAATATTTCTCCAAAACTTTTGATATGTCTAGTAAGTACATTGTTTAGAGTCGGATGTCATTCAACAATGGGTGCAGTCTGCAAATGATGCTTATGCGATGAGATAAATTTACAGCAGAAATTAATGAATCACTTTGCTATGAATCATACATATTTGCATGAATTTATGCTTTAGGTAAATAACGTGGGATCAAACTTTAGAAAGCCAACCATTGAGTACACTGCTGAAGAATATTCAGAGCTAATGACAATTAATTTAGACTCATCATTCCATTTGTGCCAACTTGCATATCCTCTTCTAAAAGAATCTGGAAATGGTAGCATTGTGTTCATTTCATCTGTTGCAGGTGTGGTTAGCTTAGGTACTGGATCTGTCTACGCAGCAAGTAAAGGTGAGTGCTACACTCATTAGTTCTTTTATTTTTTTCATGTAAATGTGATTAATAAAATGTTACGATTAAACGACAATTGTGTTTCATCCATTGAATTTTCAGCTGCAATTAATCAGCTTACAAAAAATTTGGCCTGTGAATGGGCAATAGACAACATAAGGAGCAATTGTGTTGTACCATGGGCAACCAGAACCCCACTTGTAGAACATGTAATTTTCTTGCTCCCTAACTTACTTTTTTTTTTTTTGAAGGAAACTTACTTATTTGGTTGATTGAATCCCACATAGAACCAATAAATATGGATACATAATAATACTCAAAAAAATTATATTTAGACGTCAAAATTTGATGTCAAATACGAGTGTCCTTTGAGTTTTATGTGTATTTTTAAAATGTGTGTAAGTTGTAGAAAATAAAAAAGAAATAGAGATAAACATATACGATGTCGCATATAGTAACTGTATTTTGATGTTCAAATATAATTTCTTTAGTATTATTACCATCTGTAATGTCTGCTTATGATTGATTATATGGACTAGCAGTAAATTGTTAGGATAGCATAAATTTAAATATAGTGTTTTTGAATTGATTGTGTCTAATATTTACATTTTTTCATTCATGGAAATACATGACTATTCCTATCTTCAAGTTCAATGGACATTCTTCATACTCTATCAAGCAAATCTTTTTTGTTCAAACTAACAATGGATGTCTCTTGTCTATGTGTTTGATCCAATATTTTTTTTTAATTTTTGTAGTTATTTCAAAATCAAAAGTTTGTGGATGATATTATGTCTCGAACTCCTCTTAAGCGCATTGCCGAACCCGAAGAGGTCTCCTCTTTGGTGACTTTCCTTTGCTTACCTGCTGCATCTTACATCACTGGACAAGTTATTTGTGTTGATGGGGGATTAACTGTGTTTGGATTTCAACCCAGCATGAGAATTACATGA

Protein sequence

>MS.gene63300.t1

MAAAAVETHSSRGGQRWSLTGMTALVTGGTRGIGHAIVNDLVAFGATVHTCSRTESELNKCLQEWQSKGFSVTGSVCDVSSRPQRESLIQQVASTFNGKLNIFVNNVGSNFRKPTIEYTAEEYSELMTINLDSSFHLCQLAYPLLKESGNGSIVFISSVAGVVSLGTGSVYAASKAAINQLTKNLACEWAIDNIRSNCVVPWATRTPLVEHLFQNQKFVDDIMSRTPLKRIAEPEEVSSLVTFLCLPAASYITGQVICVDGGLTVFGFQPSMRIT