AlfalfaGEDB | MS.gene63308

M. sativa cultivar XinJiangDaYe / MS.gene63308

KEGG: K08081
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63308.t1	XP_013460702.1	95.6	275	10	1	1	273	1	275	1.00E-144	522.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63308.t1	Q9LHT0	67.2	259	85	0	10	268	4	262	2.3e-97	356.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63308.t1	A0A072UZ65	95.6	275	10	1	1	273	1	275	7.4e-145	522.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049341	MS.gene63308	0.817972	2.50E-52	-1.69E-46
MS.gene055891	MS.gene63308	0.802261	6.10E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene41122	MS.gene63308	PPI
MS.gene63308	MS.gene31648	PPI
MS.gene63308	MS.gene033685	PPI
MS.gene63308	MS.gene85724	PPI
MS.gene63308	MS.gene87780	PPI
MS.gene073684	MS.gene63308	PPI
MS.gene63308	MS.gene018066	PPI
MS.gene63308	MS.gene80360	PPI
MS.gene63308	MS.gene024269	PPI
MS.gene63308	MS.gene003729	PPI
MS.gene63308	MS.gene019786	PPI
MS.gene63308	MS.gene007002	PPI
MS.gene63308	MS.gene050131	PPI
MS.gene63308	MS.gene43481	PPI
MS.gene63308	MS.gene52743	PPI
MS.gene87026	MS.gene63308	PPI
MS.gene63308	MS.gene54598	PPI
MS.gene63308	MS.gene072664	PPI
MS.gene63308	MS.gene07097	PPI
MS.gene63308	MS.gene021164	PPI
MS.gene63308	MS.gene95020	PPI
MS.gene63308	MS.gene012681	PPI
MS.gene63308	MS.gene065380	PPI
MS.gene63308	MS.gene07096	PPI
MS.gene056339	MS.gene63308	PPI
MS.gene63308	MS.gene064756	PPI
MS.gene54952	MS.gene63308	PPI
MS.gene050432	MS.gene63308	PPI
MS.gene63308	MS.gene50524	PPI
MS.gene42260	MS.gene63308	PPI
MS.gene63308	MS.gene50522	PPI
MS.gene63308	MS.gene28848	PPI
MS.gene012794	MS.gene63308	PPI
MS.gene63308	MS.gene42856	PPI
MS.gene007006	MS.gene63308	PPI
MS.gene63308	MS.gene08694	PPI
MS.gene63308	MS.gene003925	PPI
MS.gene63308	MS.gene52742	PPI
MS.gene63308	MS.gene04700	PPI
MS.gene63308	MS.gene31507	PPI
MS.gene63308	MS.gene28847	PPI
MS.gene63308	MS.gene059170	PPI
MS.gene63308	MS.gene08695	PPI
MS.gene63308	MS.gene006999	PPI
MS.gene63308	MS.gene021442	PPI
MS.gene54548	MS.gene63308	PPI
MS.gene003926	MS.gene63308	PPI
MS.gene63308	MS.gene23019	PPI
MS.gene63308	MS.gene03319	PPI
MS.gene63308	MS.gene043387	PPI
MS.gene63308	MS.gene25674	PPI
MS.gene63308	MS.gene014425	PPI
MS.gene63308	MS.gene68880	PPI
MS.gene63308	MS.gene042142	PPI
MS.gene63308	MS.gene68956	PPI
MS.gene63308	MS.gene08756	PPI
MS.gene63308	MS.gene42114	PPI
MS.gene63308	MS.gene007678	PPI
MS.gene63308	MS.gene028938	PPI
MS.gene050431	MS.gene63308	PPI
MS.gene63308	MS.gene25667	PPI
MS.gene63308	MS.gene003992	PPI
MS.gene63308	MS.gene30551	PPI
MS.gene63308	MS.gene055846	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63308.t1	MTR_3g068065	95.636	275	10	1	1	273	1	275	0.0	537
MS.gene63308.t1	MTR_3g068085	84.444	270	42	0	1	270	1	270	2.37e-167	464
MS.gene63308.t1	MTR_3g068070	81.818	264	48	0	1	264	1	264	1.06e-161	449
MS.gene63308.t1	MTR_3g068155	72.973	259	70	0	12	270	16	274	1.01e-142	402
MS.gene63308.t1	MTR_3g068175	70.221	272	79	1	1	270	1	272	3.61e-139	393
MS.gene63308.t1	MTR_3g068175	69.203	276	79	2	1	270	1	276	4.56e-137	387
MS.gene63308.t1	MTR_4g064883	59.690	258	104	0	10	267	9	266	7.07e-117	336
MS.gene63308.t1	MTR_4g064887	59.542	262	106	0	1	262	1	262	3.12e-114	329
MS.gene63308.t1	MTR_7g063320	57.647	255	104	1	9	259	9	263	1.43e-102	300
MS.gene63308.t1	MTR_7g063270	58.594	256	103	1	9	261	10	265	2.00e-102	299
MS.gene63308.t1	MTR_7g063260	56.809	257	107	1	9	261	10	266	1.45e-100	295
MS.gene63308.t1	MTR_7g063330	57.031	256	107	2	9	261	9	264	2.06e-98	289
MS.gene63308.t1	MTR_7g063360	57.031	256	106	2	9	260	10	265	7.15e-98	288
MS.gene63308.t1	MTR_7g063390	54.688	256	112	1	9	260	10	265	1.00e-96	285
MS.gene63308.t1	MTR_4g064887	61.275	204	79	0	1	204	1	204	9.07e-90	266
MS.gene63308.t1	MTR_4g064883	60.000	195	78	0	10	204	9	203	1.01e-85	255
MS.gene63308.t1	MTR_7g063270	49.219	256	78	3	9	261	10	216	8.95e-75	227
MS.gene63308.t1	MTR_4g132850	32.932	249	156	6	15	259	7	248	2.01e-34	125
MS.gene63308.t1	MTR_7g094520	33.074	257	152	10	15	259	11	259	2.10e-27	107
MS.gene63308.t1	MTR_7g094510	32.540	252	160	8	15	259	6	254	2.23e-27	106
MS.gene63308.t1	MTR_3g085740	29.508	244	161	5	21	259	81	318	1.35e-26	106
MS.gene63308.t1	MTR_7g071680	30.325	277	155	8	15	262	29	296	3.36e-25	102
MS.gene63308.t1	MTR_7g083870	30.258	271	167	8	9	261	22	288	1.57e-24	100
MS.gene63308.t1	MTR_4g058810	29.231	260	171	9	15	264	11	267	2.87e-23	96.3
MS.gene63308.t1	MTR_1196s0010	30.864	243	157	6	20	259	279	513	5.83e-23	98.2
MS.gene63308.t1	MTR_4g058840	28.846	260	172	9	15	264	11	267	8.11e-23	95.1
MS.gene63308.t1	MTR_4g058820	28.846	260	172	9	15	264	11	267	8.11e-23	95.1
MS.gene63308.t1	MTR_3g068075	88.000	50	5	1	123	171	1	50	2.17e-22	88.6
MS.gene63308.t1	MTR_7g107670	27.652	264	177	6	8	261	26	285	1.60e-21	92.0
MS.gene63308.t1	MTR_4g131740	28.464	267	179	6	6	263	31	294	1.60e-20	89.0
MS.gene63308.t1	MTR_4g078630	30.385	260	162	10	15	261	19	272	5.11e-20	87.4
MS.gene63308.t1	MTR_4g052350	29.615	260	164	10	15	264	14	264	2.33e-19	85.5
MS.gene63308.t1	MTR_6g023590	28.947	266	171	10	5	259	3	261	3.97e-19	84.7
MS.gene63308.t1	MTR_8g442750	27.481	262	163	7	15	259	14	265	4.56e-19	85.1
MS.gene63308.t1	MTR_4g052320	27.308	260	181	6	10	264	3	259	3.53e-18	82.0
MS.gene63308.t1	MTR_6g023990	29.104	268	169	11	5	259	3	262	8.38e-18	81.3
MS.gene63308.t1	MTR_1g068955	29.457	258	169	8	15	263	14	267	3.30e-17	79.7
MS.gene63308.t1	MTR_7g105590	30.108	186	123	4	15	197	8	189	4.52e-17	77.8
MS.gene63308.t1	MTR_8g089575	30.698	215	120	6	6	202	34	237	4.70e-17	79.7
MS.gene63308.t1	MTR_3g020670	29.658	263	160	10	15	260	16	270	5.66e-17	79.0
MS.gene63308.t1	MTR_4g052300	26.515	264	177	9	10	264	3	258	7.33e-17	78.6
MS.gene63308.t1	MTR_2g090165	26.848	257	176	7	11	260	2	253	1.06e-16	78.2
MS.gene63308.t1	MTR_4g052340	27.237	257	174	8	15	264	14	264	1.28e-16	77.8
MS.gene63308.t1	MTR_6g023910	28.358	268	170	10	5	259	3	261	5.04e-16	76.3
MS.gene63308.t1	MTR_7g113650	27.734	256	174	7	15	262	40	292	5.17e-16	76.6
MS.gene63308.t1	MTR_6g023600	28.195	266	173	8	5	259	3	261	6.18e-16	75.9
MS.gene63308.t1	MTR_6g023950	27.652	264	177	8	5	259	3	261	9.25e-16	75.5
MS.gene63308.t1	MTR_4g058830	30.233	172	113	6	98	264	44	213	2.21e-15	73.6
MS.gene63308.t1	MTR_1g082760	26.459	257	178	6	15	263	40	293	6.06e-15	73.6
MS.gene63308.t1	MTR_7g110130	28.244	262	167	9	11	260	2	254	7.06e-15	73.2
MS.gene63308.t1	MTR_1g051165	25.292	257	176	10	21	263	20	274	8.89e-15	72.8
MS.gene63308.t1	MTR_6g023610	27.007	274	178	10	5	265	3	267	9.55e-15	72.8
MS.gene63308.t1	MTR_7g105460	28.000	275	168	12	1	260	1	260	1.45e-14	72.4
MS.gene63308.t1	MTR_5g098650	66.000	50	17	0	221	270	124	173	4.01e-14	69.3
MS.gene63308.t1	MTR_6g023600	27.444	266	169	8	5	259	3	255	7.74e-14	70.1
MS.gene63308.t1	MTR_7g110140	26.336	262	185	6	11	267	2	260	1.02e-13	69.7
MS.gene63308.t1	MTR_7g110160	28.125	256	156	7	11	260	2	235	1.52e-13	68.9
MS.gene63308.t1	MTR_3g098470	32.653	147	97	2	8	154	25	169	2.54e-13	68.9
MS.gene63308.t1	MTR_1563s0010	28.477	151	102	2	6	151	100	249	8.93e-13	68.2
MS.gene63308.t1	MTR_3g086210	27.228	202	124	4	17	204	53	245	9.18e-13	67.4
MS.gene63308.t1	MTR_1579s0020	34.091	88	57	1	181	268	1	87	2.88e-12	61.6
MS.gene63308.t1	MTR_8g044240	26.087	207	143	5	7	204	61	266	3.59e-12	65.9
MS.gene63308.t1	MTR_7g076660	30.864	243	107	8	20	205	10	248	3.90e-12	65.5
MS.gene63308.t1	MTR_7g076660	30.864	243	107	8	20	205	9	247	4.02e-12	65.5
MS.gene63308.t1	MTR_7g080410	26.230	183	125	4	22	197	45	224	2.25e-11	63.5
MS.gene63308.t1	MTR_7g080410	26.178	191	131	4	22	205	45	232	2.30e-11	63.2
MS.gene63308.t1	MTR_1304s0010	30.994	171	111	4	93	261	43	208	3.89e-11	61.6
MS.gene63308.t1	MTR_3g113870	26.257	179	126	3	28	204	34	208	4.05e-11	62.0
MS.gene63308.t1	MTR_1g031180	31.293	147	99	2	8	154	30	174	9.58e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63308.t1	AT5G06060	67.181	259	85	0	10	268	4	262	1.52e-129	368
MS.gene63308.t1	AT2G29150	60.000	265	104	2	1	264	1	264	1.97e-115	332
MS.gene63308.t1	AT2G29350	60.755	265	101	2	1	264	1	263	2.52e-114	330
MS.gene63308.t1	AT2G29290	61.004	259	99	1	10	266	2	260	3.65e-114	329
MS.gene63308.t1	AT2G29260	60.392	255	101	0	12	266	65	319	1.19e-112	327
MS.gene63308.t1	AT2G29360	58.868	265	108	1	1	264	1	265	3.44e-111	322
MS.gene63308.t1	AT1G07440	58.846	260	104	2	9	266	6	264	5.50e-109	316
MS.gene63308.t1	AT2G29370	56.604	265	114	1	1	264	1	265	5.10e-106	308
MS.gene63308.t1	AT2G29340	57.471	261	109	1	10	268	2	262	2.82e-105	306
MS.gene63308.t1	AT2G29340	56.981	265	112	1	10	272	2	266	7.48e-105	307
MS.gene63308.t1	AT1G07450	58.893	253	103	1	12	264	5	256	5.55e-104	303
MS.gene63308.t1	AT2G30670	58.039	255	107	0	10	264	2	256	1.05e-103	302
MS.gene63308.t1	AT2G29330	58.846	260	103	3	10	266	2	260	3.29e-103	301
MS.gene63308.t1	AT2G29290	60.338	237	92	1	32	266	7	243	2.45e-101	296
MS.gene63308.t1	AT2G29310	56.471	255	111	0	10	264	2	256	1.20e-100	295
MS.gene63308.t1	AT2G29320	57.588	257	106	2	13	266	11	267	2.46e-99	291
MS.gene63308.t1	AT2G29300	55.385	260	113	2	10	266	2	261	2.62e-98	289
MS.gene63308.t1	AT2G29300	55.556	252	111	1	10	260	2	253	2.71e-95	282
MS.gene63308.t1	AT2G29350	59.735	226	88	2	40	264	2	225	2.32e-94	278
MS.gene63308.t1	AT2G29320	56.485	239	101	2	31	266	11	249	1.34e-88	264
MS.gene63308.t1	AT2G29350	60.680	206	81	0	1	206	1	206	1.59e-88	263
MS.gene63308.t1	AT2G29310	55.556	234	104	0	31	264	21	254	2.79e-88	263
MS.gene63308.t1	AT2G29340	57.143	196	84	0	10	205	2	197	1.37e-77	234
MS.gene63308.t1	AT2G29340	57.143	196	84	0	10	205	2	197	1.37e-77	234
MS.gene63308.t1	AT2G29310	56.345	197	86	0	10	206	2	198	9.40e-76	229
MS.gene63308.t1	AT2G29320	58.115	191	79	1	13	202	11	201	8.11e-74	224
MS.gene63308.t1	AT1G07440	57.317	164	70	0	9	172	6	169	4.42e-65	201
MS.gene63308.t1	AT2G29310	55.114	176	79	0	31	206	21	196	6.42e-63	196
MS.gene63308.t1	AT2G29170	60.784	102	39	1	1	101	1	102	2.49e-35	123
MS.gene63308.t1	AT4G05530	33.333	246	159	5	15	259	9	250	4.22e-34	124
MS.gene63308.t1	AT5G18210	33.333	261	159	8	10	259	3	259	9.60e-30	113
MS.gene63308.t1	AT3G03980	32.046	259	159	7	15	259	14	269	3.84e-28	109
MS.gene63308.t1	AT1G24360	30.204	245	158	6	21	259	80	317	1.70e-27	108
MS.gene63308.t1	AT4G13180	32.075	265	166	9	4	259	2	261	3.98e-27	106
MS.gene63308.t1	AT5G18210	31.765	255	150	8	14	259	7	246	4.06e-25	101
MS.gene63308.t1	AT3G04000	33.462	260	153	9	15	258	14	269	4.18e-24	98.6
MS.gene63308.t1	AT3G55310	28.295	258	174	7	15	263	17	272	3.08e-23	96.3
MS.gene63308.t1	AT3G55290	28.295	258	174	7	15	263	18	273	4.44e-23	95.9
MS.gene63308.t1	AT3G55290	28.295	258	174	7	15	263	17	272	4.69e-23	95.9
MS.gene63308.t1	AT1G54870	31.518	257	165	6	15	263	84	337	4.76e-23	96.7
MS.gene63308.t1	AT1G54870	31.518	257	165	6	15	263	82	335	5.13e-23	96.7
MS.gene63308.t1	AT3G46170	28.682	258	173	7	15	263	26	281	6.20e-22	92.8
MS.gene63308.t1	AT1G63380	27.413	259	176	6	15	263	17	273	2.32e-21	91.3
MS.gene63308.t1	AT1G62610	27.027	259	177	6	15	263	13	269	2.66e-21	90.9
MS.gene63308.t1	AT1G63380	27.413	259	176	6	15	263	15	271	2.67e-21	90.9
MS.gene63308.t1	AT1G62610	27.027	259	177	6	15	263	14	270	2.79e-21	90.9
MS.gene63308.t1	AT1G63380	27.413	259	176	6	15	263	19	275	2.85e-21	90.9
MS.gene63308.t1	AT1G62610	27.027	259	177	6	15	263	15	271	2.98e-21	90.9
MS.gene63308.t1	AT1G62610	27.027	259	177	6	15	263	19	275	3.12e-21	90.9
MS.gene63308.t1	AT1G63380	27.413	259	176	6	15	263	22	278	3.54e-21	90.9
MS.gene63308.t1	AT3G26760	27.925	265	172	6	10	260	31	290	9.18e-21	90.1
MS.gene63308.t1	AT2G17845	27.099	262	177	7	15	264	47	306	1.03e-20	90.1
MS.gene63308.t1	AT3G05260	30.469	256	166	6	15	262	37	288	1.15e-19	86.7
MS.gene63308.t1	AT2G47130	28.906	256	171	7	11	260	2	252	8.76e-19	83.6
MS.gene63308.t1	AT2G47120	27.444	266	173	11	11	267	2	256	3.41e-18	82.0
MS.gene63308.t1	AT2G47120	27.481	262	170	11	15	267	7	257	5.62e-18	81.6
MS.gene63308.t1	AT3G12800	25.098	255	179	6	15	259	10	262	9.64e-18	81.6
MS.gene63308.t1	AT1G52340	29.008	262	166	8	15	260	18	275	1.93e-17	80.5
MS.gene63308.t1	AT3G26770	27.519	258	172	5	15	261	41	294	1.39e-16	78.6
MS.gene63308.t1	AT3G42960	29.851	268	164	9	10	261	3	262	2.60e-16	77.0
MS.gene63308.t1	AT3G51680	25.940	266	175	7	15	260	32	295	4.39e-16	77.0
MS.gene63308.t1	AT3G29260	27.626	257	173	7	11	260	2	252	1.38e-14	72.0
MS.gene63308.t1	AT4G03140	24.609	256	179	5	15	260	78	329	5.80e-14	71.2
MS.gene63308.t1	AT3G06060	26.768	198	138	2	9	203	31	224	1.03e-12	67.4
MS.gene63308.t1	AT3G06060	26.768	198	138	2	9	203	48	241	1.13e-12	67.4
MS.gene63308.t1	AT4G13250	25.391	256	152	8	21	243	165	414	2.17e-12	67.0
MS.gene63308.t1	AT1G49670	28.436	211	130	7	17	211	6	211	2.30e-12	67.4
MS.gene63308.t1	AT1G49670	28.436	211	130	7	17	211	6	211	2.88e-12	67.0
MS.gene63308.t1	AT5G04900	29.444	180	119	5	18	191	80	257	3.57e-12	66.2
MS.gene63308.t1	AT1G10310	30.588	170	115	3	19	187	19	186	7.64e-12	64.3
MS.gene63308.t1	AT1G67730	24.424	217	131	6	5	205	45	244	1.04e-11	64.7
MS.gene63308.t1	AT3G47350	25.108	231	157	8	1	221	29	253	1.66e-11	63.9
MS.gene63308.t1	AT3G47350	25.108	231	157	8	1	221	29	253	2.85e-11	63.2

Find 55 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTACACGCTGCGTATA+TGG	0.258151	3.3:+60325325	None:intergenic
ACTTAATTAATCACTTCTTA+TGG	0.284170	3.3:+60324731	None:intergenic
GGACAAGTTATTTCTGTTGA+TGG	0.321935	3.3:-60324789	MS.gene63308:CDS
TATCCTCTTCTTAAAGAATC+TGG	0.324082	3.3:-60325407	MS.gene63308:CDS
AATGGGCAGAAGACAATATA+AGG	0.355538	3.3:-60325161	MS.gene63308:CDS
GTAGAGGAACTTGCTGAGTT+TGG	0.369806	3.3:-60326313	MS.gene63308:CDS
ATCACTTCTTATGGTCATGC+TGG	0.390380	3.3:+60324740	None:intergenic
TCCGTTGTATACTCAATTGT+TGG	0.398000	3.3:+60325491	None:intergenic
CTATAGTGTACACTTGTTCT+AGG	0.398286	3.3:-60326288	MS.gene63308:CDS
CTTCTTAAAGAATCTGGAAA+TGG	0.409640	3.3:-60325401	MS.gene63308:CDS
CTCGTCACCGGCGGAACTCG+TGG	0.413917	3.3:-60326352	MS.gene63308:CDS
GATATGTCAGTTGGCATAGA+TGG	0.418094	3.3:+60325426	None:intergenic
AAAATTGATGAGTATTAACT+TGG	0.439582	3.3:-60325459	MS.gene63308:CDS
TATCTCGAACACCGATAAAA+CGG	0.446152	3.3:-60324881	MS.gene63308:CDS
ACAAAGGTCTTGCTTGTGAA+TGG	0.464150	3.3:-60325179	MS.gene63308:CDS
TTGAATGAGTGGAAGGAGAA+AGG	0.480371	3.3:-60326241	MS.gene63308:CDS
TCACTTCTTATGGTCATGCT+GGG	0.488997	3.3:+60324741	None:intergenic
TCTCTGACAAACGTAGGTTC+TGG	0.495804	3.3:-60325350	MS.gene63308:CDS
TGAGCTTAGGTAAACAATGT+TGG	0.501292	3.3:-60325530	MS.gene63308:intron
TTTCCAGATTCTTTAAGAAG+AGG	0.503369	3.3:+60325404	None:intergenic
GGATCAACTGCGTTGCGCCT+TGG	0.504412	3.3:-60325140	MS.gene63308:CDS
ACATGAAGTGTCTTCCTTGG+TGG	0.509451	3.3:-60324847	MS.gene63308:CDS
ACCGGCGGAACTCGTGGGAT+CGG	0.517357	3.3:-60326346	MS.gene63308:CDS
ACCATATACGCAGCGTGTAA+AGG	0.519622	3.3:-60325326	MS.gene63308:intron
CCAGCTGCTTCTTACATCAC+TGG	0.529349	3.3:-60324810	MS.gene63308:CDS
AACACATGAAGTGTCTTCCT+TGG	0.529357	3.3:-60324850	MS.gene63308:CDS
ACCGATCCCACGAGTTCCGC+CGG	0.539004	3.3:+60326345	None:intergenic
TAAGAAGAGGATATGTCAGT+TGG	0.546406	3.3:+60325417	None:intergenic
CAAGTTATTTCTGTTGATGG+AGG	0.549415	3.3:-60324786	MS.gene63308:CDS
CAAAGGTCTTGCTTGTGAAT+GGG	0.550102	3.3:-60325178	MS.gene63308:CDS
GGATTCACAGCTCTCGTCAC+CGG	0.550668	3.3:-60326364	MS.gene63308:CDS
GATGGAGGATTTACTGCGAA+TGG	0.556013	3.3:-60324771	MS.gene63308:CDS
GACTTCTTCTTCTCAAAGAG+AGG	0.564516	3.3:-60326188	MS.gene63308:CDS
AAGAGAGGAGCTTGTTCGAA+AGG	0.571197	3.3:-60326173	MS.gene63308:CDS
AAACTCAAGATGGTCCCTCA+AGG	0.573784	3.3:-60326386	MS.gene63308:CDS
TCGTCACCGGCGGAACTCGT+GGG	0.574228	3.3:-60326351	MS.gene63308:CDS
GAGAGCTGTGAATCCCTTGA+GGG	0.579284	3.3:+60326372	None:intergenic
AACATTACATAATCATTAAG+CGG	0.585492	3.3:+60325105	None:intergenic
AGAGGAGCTTGTTCGAAAGG+TGG	0.587374	3.3:-60326170	MS.gene63308:CDS
AACTCAAGATGGTCCCTCAA+GGG	0.601552	3.3:-60326385	MS.gene63308:CDS
AAGAAGCAGCTGGCAGACAA+AGG	0.611292	3.3:+60324820	None:intergenic
GCAATCAATCAGCTGAACAA+AGG	0.614458	3.3:-60325195	MS.gene63308:CDS
CGAGAGCTGTGAATCCCTTG+AGG	0.617629	3.3:+60326371	None:intergenic
CCAGTGATGTAAGAAGCAGC+TGG	0.629413	3.3:+60324810	None:intergenic
TTCACAGCTCTCGTCACCGG+CGG	0.631222	3.3:-60326361	MS.gene63308:CDS
CAGACAAAGGAAAGCCACCA+AGG	0.631375	3.3:+60324833	None:intergenic
TGAATGAACGCTTGAATGAG+TGG	0.642583	3.3:-60326252	MS.gene63308:CDS
TGAACGCTTGAATGAGTGGA+AGG	0.643639	3.3:-60326248	MS.gene63308:CDS
CACTTGTTCTAGGAATGAAG+AGG	0.665255	3.3:-60326278	MS.gene63308:CDS
ACTGTAGCAGAAACTCAAGA+TGG	0.668815	3.3:-60326396	MS.gene63308:CDS
ACCAACAATTGAGTATACAA+CGG	0.674590	3.3:-60325492	MS.gene63308:CDS
GTTGCATCTCTGACAAACGT+AGG	0.677603	3.3:-60325356	MS.gene63308:CDS
ACAATGTTGGAACAAATGTG+AGG	0.690959	3.3:-60325517	MS.gene63308:CDS
GTTTGTCAGAGATGCAACAG+AGG	0.693930	3.3:+60325361	None:intergenic
GATCGGTCATGCTATCGTAG+AGG	0.705413	3.3:-60326329	MS.gene63308:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTGATGAGTATTAACT+TGG	-	chr3.3:60325690-60325709	MS.gene63308:intron	20.0%
!!	AACATTACATAATCATTAAG+CGG	+	chr3.3:60326047-60326066	None:intergenic	20.0%
!!	AAGCATAAGAAAATCTTAAA+TGG	+	chr3.3:60325422-60325441	None:intergenic	20.0%
!!	ACTTATTGTAGAAAATTTAG+TGG	-	chr3.3:60325164-60325183	MS.gene63308:CDS	20.0%
!!	TCAGAAAGAAAAAATGATAA+GGG	+	chr3.3:60326100-60326119	None:intergenic	20.0%
!!	TTCAGAAAGAAAAAATGATA+AGG	+	chr3.3:60326101-60326120	None:intergenic	20.0%
!	TCTAATATTCTTATGAGCTT+AGG	-	chr3.3:60325606-60325625	MS.gene63308:intron	25.0%
!	TTAAGAACATTGCATTACAT+AGG	-	chr3.3:60325283-60325302	MS.gene63308:intron	25.0%
!	TTAGCATATAACTGTAAGAA+AGG	+	chr3.3:60326225-60326244	None:intergenic	25.0%
!!	GTGCTGATAATAATTTGATT+TGG	-	chr3.3:60326134-60326153	MS.gene63308:intron	25.0%
	AACATTGCATTACATAGGTA+GGG	-	chr3.3:60325288-60325307	MS.gene63308:intron	30.0%
	ACCAACAATTGAGTATACAA+CGG	-	chr3.3:60325657-60325676	MS.gene63308:intron	30.0%
	CTTCTTAAAGAATCTGGAAA+TGG	-	chr3.3:60325748-60325767	MS.gene63308:intron	30.0%
	GTATGCTTAAACATGATTGA+TGG	-	chr3.3:60325871-60325890	MS.gene63308:intron	30.0%
	GTTTACACATGATTGGAATT+TGG	+	chr3.3:60325222-60325241	None:intergenic	30.0%
	TATCCTCTTCTTAAAGAATC+TGG	-	chr3.3:60325742-60325761	MS.gene63308:intron	30.0%
!	TTTCCAGATTCTTTAAGAAG+AGG	+	chr3.3:60325748-60325767	None:intergenic	30.0%
	AATGGGCAGAAGACAATATA+AGG	-	chr3.3:60325988-60326007	MS.gene63308:intron	35.0%
	ACAATGTTGGAACAAATGTG+AGG	-	chr3.3:60325632-60325651	MS.gene63308:intron	35.0%
	ATGACTCGTTTACACATGAT+TGG	+	chr3.3:60325229-60325248	None:intergenic	35.0%
	CTATAGTGTACACTTGTTCT+AGG	-	chr3.3:60324861-60324880	MS.gene63308:CDS	35.0%
	GAACATTGCATTACATAGGT+AGG	-	chr3.3:60325287-60325306	MS.gene63308:intron	35.0%
	GACATGAATAAGTGGAAATC+CGG	+	chr3.3:60325329-60325348	None:intergenic	35.0%
	TAAGAAGAGGATATGTCAGT+TGG	+	chr3.3:60325735-60325754	None:intergenic	35.0%
	TATCTCGAACACCGATAAAA+CGG	-	chr3.3:60326268-60326287	MS.gene63308:CDS	35.0%
	TCCGTTGTATACTCAATTGT+TGG	+	chr3.3:60325661-60325680	None:intergenic	35.0%
	TGAGCTTAGGTAAACAATGT+TGG	-	chr3.3:60325619-60325638	MS.gene63308:intron	35.0%
!	AAGGTGGCTTCTGATTTTAA+TGG	-	chr3.3:60324995-60325014	MS.gene63308:intron	35.0%
!	CTTTTGATGCACTTTGTCTT+TGG	-	chr3.3:60325396-60325415	MS.gene63308:CDS	35.0%
!	GAGAAAGGTTTTTCTGTTCA+TGG	-	chr3.3:60324923-60324942	MS.gene63308:intron	35.0%
!	GTTTCTGCTATCCGTTTTAT+CGG	+	chr3.3:60326282-60326301	None:intergenic	35.0%
!!	AAAATGATAAGGGCTCTGAA+TGG	+	chr3.3:60326090-60326109	None:intergenic	35.0%
!!	AAATGATAAGGGCTCTGAAT+GGG	+	chr3.3:60326089-60326108	None:intergenic	35.0%
!!	CAAGTTATTTCTGTTGATGG+AGG	-	chr3.3:60326363-60326382	MS.gene63308:CDS	35.0%
!!	GGACAAGTTATTTCTGTTGA+TGG	-	chr3.3:60326360-60326379	MS.gene63308:CDS	35.0%
	ACAAAGGTCTTGCTTGTGAA+TGG	-	chr3.3:60325970-60325989	MS.gene63308:intron	40.0%
	ACTGTAGCAGAAACTCAAGA+TGG	-	chr3.3:60324753-60324772	MS.gene63308:CDS	40.0%
	ATCACTTCTTATGGTCATGC+TGG	+	chr3.3:60326412-60326431	None:intergenic	40.0%
	CAAAGGTCTTGCTTGTGAAT+GGG	-	chr3.3:60325971-60325990	MS.gene63308:intron	40.0%
	CATTACATAGGTAGGGACTA+GGG	-	chr3.3:60325295-60325314	MS.gene63308:intron	40.0%
	GACTTCTTCTTCTCAAAGAG+AGG	-	chr3.3:60324961-60324980	MS.gene63308:intron	40.0%
	GATATGTCAGTTGGCATAGA+TGG	+	chr3.3:60325726-60325745	None:intergenic	40.0%
	GCAATCAATCAGCTGAACAA+AGG	-	chr3.3:60325954-60325973	MS.gene63308:intron	40.0%
	TCACTTCTTATGGTCATGCT+GGG	+	chr3.3:60326411-60326430	None:intergenic	40.0%
	TGAATGAACGCTTGAATGAG+TGG	-	chr3.3:60324897-60324916	MS.gene63308:CDS	40.0%
	TGCGACAAGACATGAATAAG+TGG	+	chr3.3:60325337-60325356	None:intergenic	40.0%
	TTGAATGAGTGGAAGGAGAA+AGG	-	chr3.3:60324908-60324927	MS.gene63308:CDS	40.0%
!	AACACATGAAGTGTCTTCCT+TGG	-	chr3.3:60326299-60326318	MS.gene63308:CDS	40.0%
!!	AGCGGTGTTTTTGTATACCA+AGG	+	chr3.3:60326029-60326048	None:intergenic	40.0%
!!	ATCGCATTTTCAAGCTTCTG+AGG	+	chr3.3:60325456-60325475	None:intergenic	40.0%
!!	CACTTGTTCTAGGAATGAAG+AGG	-	chr3.3:60324871-60324890	MS.gene63308:CDS	40.0%
!!	GGCTCTGAATGGGTAATTAA+AGG	+	chr3.3:60326079-60326098	None:intergenic	40.0%
!!	TCGCATTTTCAAGCTTCTGA+GGG	+	chr3.3:60325455-60325474	None:intergenic	40.0%
	AAACTCAAGATGGTCCCTCA+AGG	-	chr3.3:60324763-60324782	MS.gene63308:CDS	45.0%
	AACTCAAGATGGTCCCTCAA+GGG	-	chr3.3:60324764-60324783	MS.gene63308:CDS	45.0%
	ACCATATACGCAGCGTGTAA+AGG	-	chr3.3:60325823-60325842	MS.gene63308:intron	45.0%
	ACCTTTACACGCTGCGTATA+TGG	+	chr3.3:60325827-60325846	None:intergenic	45.0%
	GATGGAGGATTTACTGCGAA+TGG	-	chr3.3:60326378-60326397	MS.gene63308:CDS	45.0%
	GCATTACATAGGTAGGGACT+AGG	-	chr3.3:60325294-60325313	MS.gene63308:intron	45.0%
	GTTGCATCTCTGACAAACGT+AGG	-	chr3.3:60325793-60325812	MS.gene63308:intron	45.0%
	GTTTGTCAGAGATGCAACAG+AGG	+	chr3.3:60325791-60325810	None:intergenic	45.0%
	TCTCTGACAAACGTAGGTTC+TGG	-	chr3.3:60325799-60325818	MS.gene63308:intron	45.0%
	TGAACGCTTGAATGAGTGGA+AGG	-	chr3.3:60324901-60324920	MS.gene63308:CDS	45.0%
	TGCAGCTGAAGAAAACAGCA+CGG	+	chr3.3:60325937-60325956	None:intergenic	45.0%
!	AAGAGAGGAGCTTGTTCGAA+AGG	-	chr3.3:60324976-60324995	MS.gene63308:intron	45.0%
!	ACATGAAGTGTCTTCCTTGG+TGG	-	chr3.3:60326302-60326321	MS.gene63308:CDS	45.0%
!!	GTAGAGGAACTTGCTGAGTT+TGG	-	chr3.3:60324836-60324855	MS.gene63308:CDS	45.0%
	AAGAAGCAGCTGGCAGACAA+AGG	+	chr3.3:60326332-60326351	None:intergenic	50.0%
	CAGACAAAGGAAAGCCACCA+AGG	+	chr3.3:60326319-60326338	None:intergenic	50.0%
	CCAGCTGCTTCTTACATCAC+TGG	-	chr3.3:60326339-60326358	MS.gene63308:CDS	50.0%
	CCAGTGATGTAAGAAGCAGC+TGG	+	chr3.3:60326342-60326361	None:intergenic	50.0%
	GAGAGCTGTGAATCCCTTGA+GGG	+	chr3.3:60324780-60324799	None:intergenic	50.0%
	GATCGGTCATGCTATCGTAG+AGG	-	chr3.3:60324820-60324839	MS.gene63308:CDS	50.0%
	TGAGCTGAAGTCATGCGTGA+CGG	+	chr3.3:60325562-60325581	None:intergenic	50.0%
!	AGAGGAGCTTGTTCGAAAGG+TGG	-	chr3.3:60324979-60324998	MS.gene63308:intron	50.0%
	CGAGAGCTGTGAATCCCTTG+AGG	+	chr3.3:60324781-60324800	None:intergenic	55.0%
	GGATTCACAGCTCTCGTCAC+CGG	-	chr3.3:60324785-60324804	MS.gene63308:CDS	55.0%
!	AGGGACTAGGGTTCTAACGC+CGG	-	chr3.3:60325307-60325326	MS.gene63308:intron	55.0%
	GGATCAACTGCGTTGCGCCT+TGG	-	chr3.3:60326009-60326028	MS.gene63308:intron	60.0%
	TTCACAGCTCTCGTCACCGG+CGG	-	chr3.3:60324788-60324807	MS.gene63308:CDS	60.0%
	ACCGATCCCACGAGTTCCGC+CGG	+	chr3.3:60324807-60324826	None:intergenic	65.0%
	ACCGGCGGAACTCGTGGGAT+CGG	-	chr3.3:60324803-60324822	MS.gene63308:CDS	65.0%
	TCGTCACCGGCGGAACTCGT+GGG	-	chr3.3:60324798-60324817	MS.gene63308:CDS	65.0%
	CTCGTCACCGGCGGAACTCG+TGG	-	chr3.3:60324797-60324816	MS.gene63308:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	60324737	60326434	60324737	ID=MS.gene63308
chr3.3	mRNA	60324737	60326434	60324737	ID=MS.gene63308.t1;Parent=MS.gene63308
chr3.3	exon	60326138	60326434	60326138	ID=MS.gene63308.t1.exon1;Parent=MS.gene63308.t1
chr3.3	CDS	60326138	60326434	60326138	ID=cds.MS.gene63308.t1;Parent=MS.gene63308.t1
chr3.3	exon	60325327	60325543	60325327	ID=MS.gene63308.t1.exon2;Parent=MS.gene63308.t1
chr3.3	CDS	60325327	60325543	60325327	ID=cds.MS.gene63308.t1;Parent=MS.gene63308.t1
chr3.3	exon	60325113	60325219	60325113	ID=MS.gene63308.t1.exon3;Parent=MS.gene63308.t1
chr3.3	CDS	60325113	60325219	60325113	ID=cds.MS.gene63308.t1;Parent=MS.gene63308.t1
chr3.3	exon	60324737	60324937	60324737	ID=MS.gene63308.t1.exon4;Parent=MS.gene63308.t1
chr3.3	CDS	60324737	60324937	60324737	ID=cds.MS.gene63308.t1;Parent=MS.gene63308.t1

Gene Sequence

>MS.gene63308

ATGGCAAAGCAAGAGAACTGTAGCAGAAACTCAAGATGGTCCCTCAAGGGATTCACAGCTCTCGTCACCGGCGGAACTCGTGGGATCGGTCATGCTATCGTAGAGGAACTTGCTGAGTTTGGTGCTATAGTGTACACTTGTTCTAGGAATGAAGAGGAGCTGAATGAACGCTTGAATGAGTGGAAGGAGAAAGGTTTTTCTGTTCATGGATCAGTTTGTGATGTGACTTCTTCTTCTCAAAGAGAGGAGCTTGTTCGAAAGGTGGCTTCTGATTTTAATGGCAAGCTCAACATACTTGTGAGTTTTTTCTTTTCTATATATTTTGTTTTTCATTTCTATATTATGTTGATGTTGAAATAAGTCTAAATCAGCTTTTAATTCAAGAAAGTGACTCATACAAATATACAATACATATATTCGTTTTTCAACTTATTGTAGAAAATTTAGTGGAATTTTTTTTCAGAATATATTTTCATACACGTCCAAATTCCAATCATGTGTAAACGAGTCATTAAAAGACTCTTAATCGTGTCATGAACTCAGTTGTTAAGAACATTGCATTACATAGGTAGGGACTAGGGTTCTAACGCCGGATTTCCACTTATTCATGTCTTGTCGCACACATTTTAACTTGCAAGTATGCGTAACAAAATTGTAATCTTTTGATGCACTTTGTCTTTGGCCATTTAAGATTTTCTTATGCTTGCTTCAAATGCCCTCAGAAGCTTGAAAATGCGATCTCTATACATACTCTCTATTTGTATTTAACAATTTTAGCATAAAGCTTAAAACAAGAAATGTTCAAATTGCTAGACTTCTTTTCCGTCACGCATGACTTCAGCTCATACTAAATTGTGCTCATTTCATAATCTAATATTCTTATGAGCTTAGGTAAACAATGTTGGAACAAATGTGAGGAAACCAACAATTGAGTATACAACGGAAGATTATTCAAAATTGATGAGTATTAACTTGGATTCTGCATACCATCTATGCCAACTGACATATCCTCTTCTTAAAGAATCTGGAAATGGAAGCATTGTATTCAATTCCTCTGTTGCATCTCTGACAAACGTAGGTTCTGGAACCATATACGCAGCGTGTAAAGGTAACTCTATGTATCATTATGTCATTGTATGCTTAAACATGATTGATGGACACAAACTATTTTGACATTTACTAAAGAGATTCGATTCACCGTGCTGTTTTCTTCAGCTGCAATCAATCAGCTGAACAAAGGTCTTGCTTGTGAATGGGCAGAAGACAATATAAGGATCAACTGCGTTGCGCCTTGGTATACAAAAACACCGCTTAATGATTATGTAATGTTCTTTTCATACCTTTAATTACCCATTCAGAGCCCTTATCATTTTTTCTTTCTGAAGTTAGTTAAGTTTGTGCTGATAATAATTTGATTTGGATGAAAAAAGTGAGTAATCTTCAAATGACAATGTTATGTTCATATCTTGTGTTGCTAATTGTTTACCTTTCTTACAGTTATATGCTAACAAAGAGTTTGTGAATGAAGTACTATCTCGAACACCGATAAAACGGATAGCAGAAACACATGAAGTGTCTTCCTTGGTGGCTTTCCTTTGTCTGCCAGCTGCTTCTTACATCACTGGACAAGTTATTTCTGTTGATGGAGGATTTACTGCGAATGGATTTCAACCCAGCATGACCATAAGAAGTGATTAA

Protein sequence

>MS.gene63308.t1

MAKQENCSRNSRWSLKGFTALVTGGTRGIGHAIVEELAEFGAIVYTCSRNEEELNERLNEWKEKGFSVHGSVCDVTSSSQREELVRKVASDFNGKLNILVNNVGTNVRKPTIEYTTEDYSKLMSINLDSAYHLCQLTYPLLKESGNGSIVFNSSVASLTNVGSGTIYAACKAAINQLNKGLACEWAEDNIRINCVAPWYTKTPLNDYLYANKEFVNEVLSRTPIKRIAETHEVSSLVAFLCLPAASYITGQVISVDGGFTANGFQPSMTIRSD