AlfalfaGEDB | MS.gene63482

M. sativa cultivar XinJiangDaYe / MS.gene63482

KEGG: K08081
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63482.t1	XP_013460702.1	95.6	274	10	1	1	272	1	274	8.30E-143	516.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63482.t1	Q9LHT0	68.1	257	82	0	12	268	6	262	7.0e-98	358.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63482.t1	A0A072UZ65	95.6	274	10	1	1	272	1	274	6.0e-143	516.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049328	MS.gene63482	0.820961	5.20E-53	-1.69E-46
MS.gene049418	MS.gene63482	0.834314	3.22E-56	-1.69E-46
MS.gene050310	MS.gene63482	0.800812	1.21E-48	-1.69E-46
MS.gene051263	MS.gene63482	0.804884	1.74E-49	-1.69E-46
MS.gene052732	MS.gene63482	0.813344	2.68E-51	-1.69E-46
MS.gene054584	MS.gene63482	0.802474	5.52E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene63482	MS.gene028938	PPI
MS.gene63482	MS.gene003992	PPI
MS.gene54548	MS.gene63482	PPI
MS.gene63482	MS.gene055846	PPI
MS.gene065380	MS.gene63482	PPI
MS.gene08694	MS.gene63482	PPI
MS.gene064756	MS.gene63482	PPI
MS.gene42856	MS.gene63482	PPI
MS.gene003926	MS.gene63482	PPI
MS.gene52743	MS.gene63482	PPI
MS.gene021442	MS.gene63482	PPI
MS.gene50522	MS.gene63482	PPI
MS.gene007678	MS.gene63482	PPI
MS.gene63482	MS.gene024269	PPI
MS.gene63482	MS.gene003729	PPI
MS.gene63482	MS.gene007002	PPI
MS.gene63482	MS.gene050131	PPI
MS.gene63482	MS.gene43481	PPI
MS.gene31648	MS.gene63482	PPI
MS.gene006999	MS.gene63482	PPI
MS.gene021164	MS.gene63482	PPI
MS.gene63482	MS.gene07097	PPI
MS.gene63482	MS.gene31507	PPI
MS.gene63482	MS.gene059170	PPI
MS.gene63482	MS.gene95020	PPI
MS.gene050431	MS.gene63482	PPI
MS.gene019786	MS.gene63482	PPI
MS.gene043387	MS.gene63482	PPI
MS.gene003925	MS.gene63482	PPI
MS.gene25667	MS.gene63482	PPI
MS.gene85724	MS.gene63482	PPI
MS.gene41122	MS.gene63482	PPI
MS.gene63482	MS.gene50524	PPI
MS.gene073684	MS.gene63482	PPI
MS.gene80360	MS.gene63482	PPI
MS.gene033685	MS.gene63482	PPI
MS.gene042142	MS.gene63482	PPI
MS.gene23019	MS.gene63482	PPI
MS.gene014425	MS.gene63482	PPI
MS.gene63482	MS.gene04700	PPI
MS.gene63482	MS.gene08695	PPI
MS.gene018066	MS.gene63482	PPI
MS.gene28848	MS.gene63482	PPI
MS.gene68956	MS.gene63482	PPI
MS.gene63482	MS.gene42114	PPI
MS.gene056339	MS.gene63482	PPI
MS.gene07096	MS.gene63482	PPI
MS.gene54952	MS.gene63482	PPI
MS.gene63482	MS.gene30551	PPI
MS.gene050432	MS.gene63482	PPI
MS.gene63482	MS.gene03319	PPI
MS.gene42260	MS.gene63482	PPI
MS.gene012794	MS.gene63482	PPI
MS.gene52742	MS.gene63482	PPI
MS.gene63482	MS.gene25674	PPI
MS.gene63482	MS.gene68880	PPI
MS.gene007006	MS.gene63482	PPI
MS.gene072664	MS.gene63482	PPI
MS.gene87026	MS.gene63482	PPI
MS.gene28847	MS.gene63482	PPI
MS.gene63482	MS.gene08756	PPI
MS.gene87780	MS.gene63482	PPI
MS.gene012681	MS.gene63482	PPI
MS.gene54598	MS.gene63482	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63482.t1	MTR_3g068065	94.161	274	14	1	1	272	1	274	0.0	517
MS.gene63482.t1	MTR_3g068085	86.296	270	37	0	1	270	1	270	4.72e-170	472
MS.gene63482.t1	MTR_3g068070	83.333	264	44	0	1	264	1	264	5.08e-165	459
MS.gene63482.t1	MTR_3g068155	73.208	265	70	1	7	270	10	274	2.20e-144	407
MS.gene63482.t1	MTR_3g068175	72.308	260	72	0	11	270	13	272	6.01e-139	394
MS.gene63482.t1	MTR_3g068175	71.212	264	72	1	11	270	13	276	1.06e-136	388
MS.gene63482.t1	MTR_4g064883	60.853	258	101	0	12	269	11	268	8.03e-119	342
MS.gene63482.t1	MTR_4g064887	60.687	262	103	0	1	262	1	262	9.82e-117	337
MS.gene63482.t1	MTR_7g063320	57.034	263	109	1	1	259	1	263	4.93e-104	305
MS.gene63482.t1	MTR_7g063270	59.073	259	103	1	6	261	7	265	1.05e-103	304
MS.gene63482.t1	MTR_7g063260	57.308	260	107	1	6	261	7	266	6.99e-102	300
MS.gene63482.t1	MTR_7g063330	57.812	256	105	2	9	261	9	264	1.60e-99	293
MS.gene63482.t1	MTR_7g063360	57.143	259	107	2	6	260	7	265	6.74e-99	292
MS.gene63482.t1	MTR_7g063390	55.212	259	112	1	6	260	7	265	3.24e-98	290
MS.gene63482.t1	MTR_4g064887	62.745	204	76	0	1	204	1	204	3.65e-92	273
MS.gene63482.t1	MTR_4g064883	61.658	193	74	0	12	204	11	203	3.32e-87	260
MS.gene63482.t1	MTR_7g063270	49.807	259	78	3	6	261	7	216	2.65e-76	233
MS.gene63482.t1	MTR_4g132850	33.333	249	155	6	15	259	7	248	6.28e-34	125
MS.gene63482.t1	MTR_7g094520	33.463	257	151	10	15	259	11	259	1.47e-28	110
MS.gene63482.t1	MTR_7g094510	32.937	252	159	8	15	259	6	254	1.92e-28	110
MS.gene63482.t1	MTR_3g085740	30.328	244	159	5	21	259	81	318	6.40e-27	107
MS.gene63482.t1	MTR_7g071680	30.986	284	158	8	8	262	22	296	6.41e-26	104
MS.gene63482.t1	MTR_7g083870	30.996	271	165	8	9	261	22	288	3.01e-25	103
MS.gene63482.t1	MTR_4g058810	29.615	260	170	9	15	264	11	267	2.73e-23	97.1
MS.gene63482.t1	MTR_4g058840	29.231	260	171	9	15	264	11	267	7.40e-23	95.9
MS.gene63482.t1	MTR_4g058820	29.231	260	171	9	15	264	11	267	7.40e-23	95.9
MS.gene63482.t1	MTR_1196s0010	30.864	243	157	6	20	259	279	513	1.43e-22	98.2
MS.gene63482.t1	MTR_3g068075	88.000	50	5	1	123	171	1	50	3.72e-22	88.6
MS.gene63482.t1	MTR_7g107670	28.302	265	175	7	7	261	26	285	1.28e-21	93.2
MS.gene63482.t1	MTR_4g078630	30.769	260	161	10	15	261	19	272	7.50e-20	87.8
MS.gene63482.t1	MTR_4g052350	29.615	260	164	10	15	264	14	264	6.60e-19	85.1
MS.gene63482.t1	MTR_8g442750	27.863	262	162	7	15	259	14	265	8.25e-19	85.1
MS.gene63482.t1	MTR_4g131740	28.090	267	180	6	6	263	31	294	1.04e-18	84.7
MS.gene63482.t1	MTR_1g068955	29.845	258	168	8	15	263	14	267	1.56e-18	84.3
MS.gene63482.t1	MTR_6g023590	28.571	266	172	10	5	259	3	261	6.31e-18	82.0
MS.gene63482.t1	MTR_3g020670	30.216	278	168	11	1	260	1	270	9.83e-18	82.0
MS.gene63482.t1	MTR_6g023910	29.478	268	167	10	5	259	3	261	3.48e-17	80.1
MS.gene63482.t1	MTR_4g052320	27.059	255	178	6	15	264	8	259	3.49e-17	80.1
MS.gene63482.t1	MTR_6g023990	28.679	265	169	10	7	259	6	262	5.34e-17	79.7
MS.gene63482.t1	MTR_2g090165	27.444	266	180	8	11	268	2	262	8.20e-17	79.0
MS.gene63482.t1	MTR_7g105580	26.740	273	166	9	15	278	8	255	1.13e-16	78.6
MS.gene63482.t1	MTR_8g089575	31.683	202	111	5	17	202	47	237	1.53e-16	79.0
MS.gene63482.t1	MTR_7g105590	29.570	186	124	4	15	197	8	189	1.53e-16	77.0
MS.gene63482.t1	MTR_4g052340	27.237	257	174	8	15	264	14	264	3.60e-16	77.4
MS.gene63482.t1	MTR_7g113650	28.125	256	173	7	15	262	40	292	5.51e-16	77.0
MS.gene63482.t1	MTR_4g052300	26.255	259	174	9	15	264	8	258	1.38e-15	75.5
MS.gene63482.t1	MTR_4g058830	31.707	164	105	6	106	264	52	213	2.34e-15	73.9
MS.gene63482.t1	MTR_6g023610	27.473	273	178	9	5	265	3	267	3.36e-15	74.7
MS.gene63482.t1	MTR_7g110130	28.676	272	172	10	11	269	2	264	4.21e-15	74.3
MS.gene63482.t1	MTR_6g023600	27.820	266	174	8	5	259	3	261	7.24e-15	73.6
MS.gene63482.t1	MTR_1g051165	25.681	257	175	10	21	263	20	274	8.42e-15	73.6
MS.gene63482.t1	MTR_6g023950	27.273	264	178	8	5	259	3	261	1.05e-14	73.2
MS.gene63482.t1	MTR_7g105460	28.736	261	158	11	15	260	13	260	2.26e-14	72.4
MS.gene63482.t1	MTR_7g110140	26.515	264	186	6	11	269	2	262	4.20e-14	71.2
MS.gene63482.t1	MTR_1g082760	26.459	257	178	6	15	263	40	293	8.89e-14	70.9
MS.gene63482.t1	MTR_5g098650	66.000	50	17	0	221	270	124	173	1.06e-13	68.6
MS.gene63482.t1	MTR_7g110160	28.516	256	155	7	11	260	2	235	1.26e-13	69.7
MS.gene63482.t1	MTR_3g098470	32.653	147	97	2	8	154	25	169	6.54e-13	68.6
MS.gene63482.t1	MTR_6g023600	27.068	266	170	8	5	259	3	255	7.28e-13	67.8
MS.gene63482.t1	MTR_8g044240	27.273	187	129	4	15	195	69	254	1.03e-12	68.2
MS.gene63482.t1	MTR_3g086210	27.723	202	123	4	17	204	53	245	1.30e-12	67.8
MS.gene63482.t1	MTR_3g113870	26.816	179	125	3	28	204	34	208	1.29e-11	63.9
MS.gene63482.t1	MTR_1579s0020	34.091	88	57	1	181	268	1	87	1.74e-11	60.1
MS.gene63482.t1	MTR_1275s0010	29.060	117	83	0	139	255	259	375	5.70e-11	63.5
MS.gene63482.t1	MTR_7g080410	26.776	183	124	4	22	197	45	224	5.91e-11	62.8
MS.gene63482.t1	MTR_7g080410	26.702	191	130	4	22	205	45	232	6.39e-11	62.4
MS.gene63482.t1	MTR_4g134660	27.219	169	120	2	19	184	10	178	8.96e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63482.t1	AT5G06060	68.093	257	82	0	12	268	6	262	2.35e-130	372
MS.gene63482.t1	AT2G29150	60.769	260	101	1	5	264	6	264	5.15e-115	333
MS.gene63482.t1	AT2G29290	61.479	257	97	1	12	266	4	260	2.57e-114	331
MS.gene63482.t1	AT2G29260	61.176	255	99	0	12	266	65	319	1.89e-113	331
MS.gene63482.t1	AT2G29350	60.000	265	103	2	1	264	1	263	4.80e-113	328
MS.gene63482.t1	AT2G29360	58.868	265	108	1	1	264	1	265	5.82e-111	323
MS.gene63482.t1	AT1G07440	58.555	263	106	2	6	266	3	264	7.78e-110	320
MS.gene63482.t1	AT2G29370	56.604	265	114	1	1	264	1	265	3.53e-106	310
MS.gene63482.t1	AT2G29340	58.301	259	106	1	12	268	4	262	5.42e-106	310
MS.gene63482.t1	AT2G29340	58.755	257	104	1	12	266	4	260	1.90e-105	310
MS.gene63482.t1	AT2G30670	58.498	253	105	0	12	264	4	256	1.74e-104	306
MS.gene63482.t1	AT1G07450	58.893	253	103	1	12	264	5	256	1.78e-104	306
MS.gene63482.t1	AT2G29330	59.690	258	100	3	12	266	4	260	4.08e-104	305
MS.gene63482.t1	AT2G29310	57.708	253	107	0	12	264	4	256	6.61e-102	300
MS.gene63482.t1	AT2G29290	60.084	238	93	1	31	266	6	243	1.08e-101	298
MS.gene63482.t1	AT2G29320	57.977	257	105	2	13	266	11	267	2.38e-100	296
MS.gene63482.t1	AT2G29300	56.202	258	110	2	12	266	4	261	4.81e-99	292
MS.gene63482.t1	AT2G29300	56.400	250	108	1	12	260	4	253	5.89e-96	285
MS.gene63482.t1	AT2G29350	59.735	226	88	2	40	264	2	225	1.24e-94	280
MS.gene63482.t1	AT2G29320	56.904	239	100	2	31	266	11	249	1.50e-89	268
MS.gene63482.t1	AT2G29310	56.410	234	102	0	31	264	21	254	2.30e-89	268
MS.gene63482.t1	AT2G29350	59.709	206	83	0	1	206	1	206	4.17e-87	260
MS.gene63482.t1	AT2G29340	57.949	195	82	0	12	206	4	198	2.30e-78	237
MS.gene63482.t1	AT2G29340	57.949	195	82	0	12	206	4	198	2.30e-78	237
MS.gene63482.t1	AT2G29310	57.949	195	82	0	12	206	4	198	7.08e-77	233
MS.gene63482.t1	AT2G29320	58.639	191	78	1	13	202	11	201	1.13e-74	228
MS.gene63482.t1	AT1G07440	57.229	166	71	0	7	172	4	169	5.58e-65	202
MS.gene63482.t1	AT2G29310	56.250	176	77	0	31	206	21	196	5.18e-64	201
MS.gene63482.t1	AT2G29170	60.204	98	39	0	4	101	5	102	1.24e-34	122
MS.gene63482.t1	AT4G05530	33.740	246	158	5	15	259	9	250	9.67e-34	124
MS.gene63482.t1	AT5G18210	33.716	261	158	8	10	259	3	259	5.11e-31	117
MS.gene63482.t1	AT3G03980	33.077	260	155	9	15	259	14	269	4.91e-29	112
MS.gene63482.t1	AT4G13180	33.208	265	162	10	5	259	2	261	1.35e-28	111
MS.gene63482.t1	AT1G24360	30.204	245	158	7	21	259	80	317	5.83e-27	108
MS.gene63482.t1	AT5G18210	32.567	261	148	9	10	259	3	246	3.20e-26	105
MS.gene63482.t1	AT3G04000	33.086	269	160	9	6	258	5	269	1.32e-23	97.8
MS.gene63482.t1	AT3G55310	28.185	259	175	7	15	264	17	273	1.74e-22	95.1
MS.gene63482.t1	AT3G55290	28.185	259	175	7	15	264	18	274	3.21e-22	94.4
MS.gene63482.t1	AT3G55290	28.185	259	175	7	15	264	17	273	3.25e-22	94.4
MS.gene63482.t1	AT1G63380	27.473	273	186	6	1	263	1	271	2.87e-21	91.7
MS.gene63482.t1	AT1G62610	27.106	273	187	6	1	263	1	271	3.23e-21	91.7
MS.gene63482.t1	AT3G46170	28.682	258	173	7	15	263	26	281	3.26e-21	91.7
MS.gene63482.t1	AT1G54870	31.128	257	166	6	15	263	82	335	3.81e-21	92.4
MS.gene63482.t1	AT1G54870	31.128	257	166	6	15	263	84	337	3.98e-21	92.4
MS.gene63482.t1	AT1G63380	27.799	259	175	6	15	263	17	273	4.01e-21	91.3
MS.gene63482.t1	AT1G62610	27.413	259	176	6	15	263	14	270	4.43e-21	91.3
MS.gene63482.t1	AT1G63380	27.799	259	175	6	15	263	19	275	4.58e-21	91.3
MS.gene63482.t1	AT1G62610	27.413	259	176	6	15	263	13	269	4.97e-21	90.9
MS.gene63482.t1	AT3G26760	28.839	267	170	7	8	260	30	290	5.61e-21	91.3
MS.gene63482.t1	AT1G62610	27.413	259	176	6	15	263	19	275	5.82e-21	90.9
MS.gene63482.t1	AT1G63380	27.799	259	175	6	15	263	22	278	7.32e-21	90.9
MS.gene63482.t1	AT2G17845	27.481	262	176	7	15	264	47	306	1.57e-20	90.5
MS.gene63482.t1	AT2G47130	29.688	256	169	7	11	260	2	252	1.29e-19	86.7
MS.gene63482.t1	AT2G47120	28.195	266	171	11	11	267	2	256	6.47e-19	84.7
MS.gene63482.t1	AT2G47120	28.244	262	168	11	15	267	7	257	9.43e-19	84.3
MS.gene63482.t1	AT3G05260	30.859	256	167	6	15	263	37	289	1.91e-18	84.0
MS.gene63482.t1	AT1G52340	30.451	266	157	10	15	260	18	275	1.03e-17	82.0
MS.gene63482.t1	AT3G12800	25.490	255	178	6	15	259	10	262	1.25e-17	82.0
MS.gene63482.t1	AT3G51680	26.692	266	173	7	15	260	32	295	1.32e-16	79.0
MS.gene63482.t1	AT3G42960	29.730	259	158	9	19	261	12	262	1.92e-16	78.2
MS.gene63482.t1	AT3G26770	27.132	258	173	5	15	261	41	294	1.28e-15	76.3
MS.gene63482.t1	AT3G29260	28.405	257	171	7	11	260	2	252	2.01e-15	75.1
MS.gene63482.t1	AT4G03140	25.391	256	177	5	15	260	78	329	9.22e-15	74.3
MS.gene63482.t1	AT5G04900	30.435	184	120	5	18	195	80	261	2.78e-13	70.1
MS.gene63482.t1	AT1G49670	28.910	211	129	7	17	211	6	211	9.90e-13	68.9
MS.gene63482.t1	AT1G49670	28.910	211	129	7	17	211	6	211	1.14e-12	68.9
MS.gene63482.t1	AT4G13250	26.070	257	151	8	20	243	164	414	3.45e-12	67.4
MS.gene63482.t1	AT3G06060	26.768	198	138	2	9	203	31	224	6.87e-12	65.9
MS.gene63482.t1	AT3G06060	26.768	198	138	2	9	203	48	241	6.98e-12	65.9
MS.gene63482.t1	AT1G10310	30.539	167	113	3	19	184	19	183	1.21e-11	63.9
MS.gene63482.t1	AT5G65205	28.090	178	121	4	21	197	12	183	9.17e-11	62.0

Find 58 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTACACGCTGCGTATA+TGG	0.258151	3.1:+56370085	None:intergenic
AATGGGCAAAAGACAATATA+AGG	0.317797	3.1:-56369921	MS.gene63482:CDS
GGACAAGTTATTTCTGTTGA+TGG	0.321935	3.1:-56369549	MS.gene63482:CDS
TATCCTCTTCTTAAAGAATC+TGG	0.324082	3.1:-56370167	MS.gene63482:CDS
GTAGAGGAACTTGCTGAGTT+TGG	0.369806	3.1:-56371086	MS.gene63482:CDS
AAAGGGTCACATACTCTTGC+TGG	0.388378	3.1:-56369227	MS.gene63482:CDS
GGAAGACACTTCATGTGTTT+CGG	0.394739	3.1:+56369614	None:intergenic
TCCGTTGTATACTCAATTGT+TGG	0.398000	3.1:+56370251	None:intergenic
CTATAGTGTACACTTGTTCT+AGG	0.398286	3.1:-56371061	MS.gene63482:CDS
CTTCTTAAAGAATCTGGAAA+TGG	0.409640	3.1:-56370161	MS.gene63482:CDS
GATATGTCAGTTGGCATAGA+TGG	0.418094	3.1:+56370186	None:intergenic
TATGTAGTATCTGTATCATT+AGG	0.427761	3.1:+56369275	None:intergenic
GATGGAGGATTTACTGCAAA+TGG	0.433393	3.1:-56369531	MS.gene63482:CDS
AAAATTGATGAGTATTAACT+TGG	0.439582	3.1:-56370219	MS.gene63482:CDS
ATCACTTCTTATGATCATGC+TGG	0.440165	3.1:+56369500	None:intergenic
CATGATGAGATGCTTGATAA+AGG	0.466551	3.1:-56369305	MS.gene63482:CDS
CTCGTCACCGGTGGAACTCG+TGG	0.467504	3.1:-56371125	MS.gene63482:CDS
TATCTCGTACACCGATAAAA+CGG	0.472351	3.1:-56369641	MS.gene63482:CDS
TTGAATGAGTGGAAGGAGAA+AGG	0.480371	3.1:-56371014	MS.gene63482:CDS
AAGGTAGCTTCTGATTTCAA+TGG	0.491807	3.1:-56370927	MS.gene63482:CDS
TCTCTGACAAACGTAGGTTC+TGG	0.495804	3.1:-56370110	MS.gene63482:CDS
AACATTACATAATCGTTAAG+TGG	0.496146	3.1:+56369865	None:intergenic
TGAGCTTAGGTAAACAATGT+TGG	0.501292	3.1:-56370290	MS.gene63482:intron
ACCGGTGGAACTCGTGGGAT+CGG	0.503309	3.1:-56371119	MS.gene63482:CDS
TTTCCAGATTCTTTAAGAAG+AGG	0.503369	3.1:+56370164	None:intergenic
TTGCAAGTGGAAAGCTCAAA+GGG	0.506249	3.1:-56369244	MS.gene63482:CDS
AATAACTTGTCCAGTGATGT+AGG	0.517499	3.1:+56369560	None:intergenic
ACCATATACGCAGCGTGTAA+AGG	0.519622	3.1:-56370086	MS.gene63482:intron
CCAGCTGCTTCCTACATCAC+TGG	0.520639	3.1:-56369570	MS.gene63482:CDS
TCACTTCTTATGATCATGCT+GGG	0.528359	3.1:+56369501	None:intergenic
CTTGCAAGTGGAAAGCTCAA+AGG	0.529045	3.1:-56369245	MS.gene63482:CDS
AACACATGAAGTGTCTTCCT+TGG	0.534095	3.1:-56369610	MS.gene63482:CDS
ACATATGATATTCTTGCAAG+TGG	0.540608	3.1:-56369257	MS.gene63482:CDS
AAGAGAGGAGCTTGTTCGAA+AGG	0.543574	3.1:-56370946	MS.gene63482:CDS
TAAGAAGAGGATATGTCAGT+TGG	0.546406	3.1:+56370177	None:intergenic
CAAGTTATTTCTGTTGATGG+AGG	0.549415	3.1:-56369546	MS.gene63482:CDS
ACCGATCCCACGAGTTCCAC+CGG	0.553414	3.1:+56371118	None:intergenic
GACTTCTTCTTCTCAAAGAG+AGG	0.564516	3.1:-56370961	MS.gene63482:CDS
GGATTCACAGCTCTCGTCAC+CGG	0.571734	3.1:-56371137	MS.gene63482:CDS
AAGCTCAAGATGGTCCCTCA+AGG	0.573784	3.1:-56371159	MS.gene63482:CDS
GAGAGCTGTGAATCCCTTGA+GGG	0.579284	3.1:+56371145	None:intergenic
CCAGTGATGTAGGAAGCAGC+TGG	0.588298	3.1:+56369570	None:intergenic
AGCTCAAGATGGTCCCTCAA+GGG	0.601552	3.1:-56371158	MS.gene63482:CDS
AGGAAGCAGCTGGCAGACAA+AGG	0.611292	3.1:+56369580	None:intergenic
TCGTCACCGGTGGAACTCGT+GGG	0.613856	3.1:-56371124	MS.gene63482:CDS
TTCACAGCTCTCGTCACCGG+TGG	0.616296	3.1:-56371134	MS.gene63482:CDS
CGAGAGCTGTGAATCCCTTG+AGG	0.617629	3.1:+56371144	None:intergenic
ACAGTAGCAGAAGCTCAAGA+TGG	0.618803	3.1:-56371169	MS.gene63482:CDS
CAGACAAAGGAAAGCTACCA+AGG	0.625310	3.1:+56369593	None:intergenic
TGAATGAACGCTTGAATGAG+TGG	0.642583	3.1:-56371025	MS.gene63482:CDS
TGAACGCTTGAATGAGTGGA+AGG	0.643639	3.1:-56371021	MS.gene63482:CDS
CACTTGTTCTAGGAATGAAG+AGG	0.665255	3.1:-56371051	MS.gene63482:CDS
ACCAACAATTGAGTATACAA+CGG	0.674590	3.1:-56370252	MS.gene63482:CDS
GTTGCATCTCTGACAAACGT+AGG	0.677603	3.1:-56370116	MS.gene63482:CDS
ACAATGTTGGAACAAATGTG+AGG	0.690959	3.1:-56370277	MS.gene63482:CDS
GTTTGTCAGAGATGCAACAG+AGG	0.693930	3.1:+56370121	None:intergenic
GATCGGTCATGCTATCGTAG+AGG	0.705413	3.1:-56371102	MS.gene63482:CDS
GGAGCAACTGTGTTGCACCG+TGG	0.740002	3.1:-56369900	MS.gene63482:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTATGTTGATGTTTAAAT+AGG	-	chr3.1:56369558-56369577	MS.gene63482:CDS	15.0%
!!	AAAATTGATGAGTATTAACT+TGG	-	chr3.1:56370186-56370205	MS.gene63482:CDS	20.0%
!!	AAGCATAAGAAAATCTTAAA+TGG	+	chr3.1:56369918-56369937	None:intergenic	20.0%
!!	ACTTATTGTAGAAAATTTAG+TGG	-	chr3.1:56369639-56369658	MS.gene63482:CDS	20.0%
!!!	CATTTTTTCTTTATGAAGAT+TGG	-	chr3.1:56370600-56370619	MS.gene63482:intron	20.0%
!!!	TGTACTGATATAATTTGATT+TGG	-	chr3.1:56370630-56370649	MS.gene63482:intron	20.0%
!	AACATTACATAATCGTTAAG+TGG	+	chr3.1:56370543-56370562	None:intergenic	25.0%
!	AACATTGCATTACATATGAA+GGG	-	chr3.1:56369765-56369784	MS.gene63482:intron	25.0%
!	GTTTACACATGATTGAAATT+TGG	+	chr3.1:56369698-56369717	None:intergenic	25.0%
!	TATGTAGTATCTGTATCATT+AGG	+	chr3.1:56371133-56371152	None:intergenic	25.0%
!	TCTTATATTCTTATGAGCTT+AGG	-	chr3.1:56370102-56370121	MS.gene63482:CDS	25.0%
!	TTAGCATATAACTGTAAGAA+AGG	+	chr3.1:56370721-56370740	None:intergenic	25.0%
!!	GACATGAAATTCACATTTTA+AGG	+	chr3.1:56369825-56369844	None:intergenic	25.0%
!!!	AGAAATAGTCTTATGTTCTA+TGG	+	chr3.1:56371026-56371045	None:intergenic	25.0%
	AAAAAGTCTTGCTTGTGAAT+GGG	-	chr3.1:56370467-56370486	MS.gene63482:intron	30.0%
	AATGGGCAAAAGACAATATA+AGG	-	chr3.1:56370484-56370503	MS.gene63482:intron	30.0%
	ACATATGATATTCTTGCAAG+TGG	-	chr3.1:56371148-56371167	MS.gene63482:CDS	30.0%
	ACCAACAATTGAGTATACAA+CGG	-	chr3.1:56370153-56370172	MS.gene63482:CDS	30.0%
	CTTCTTAAAGAATCTGGAAA+TGG	-	chr3.1:56370244-56370263	MS.gene63482:CDS	30.0%
	GAACATTGCATTACATATGA+AGG	-	chr3.1:56369764-56369783	MS.gene63482:intron	30.0%
	GTATGCTTAAACATGATTGA+TGG	-	chr3.1:56370367-56370386	MS.gene63482:intron	30.0%
	TATCCTCTTCTTAAAGAATC+TGG	-	chr3.1:56370238-56370257	MS.gene63482:CDS	30.0%
!	TTTCCAGATTCTTTAAGAAG+AGG	+	chr3.1:56370244-56370263	None:intergenic	30.0%
!!	AAAATGATAAGAGCTCTGAA+TGG	+	chr3.1:56370586-56370605	None:intergenic	30.0%
!!	AGCTCTGAATGGATAATTAA+AGG	+	chr3.1:56370575-56370594	None:intergenic	30.0%
!!!	CACATTTTAAGGTGAATAAG+TGG	+	chr3.1:56369814-56369833	None:intergenic	30.0%
	AAGGTAGCTTCTGATTTCAA+TGG	-	chr3.1:56369478-56369497	MS.gene63482:intron	35.0%
	AAGGTGAATAAGTGGAAATC+CGG	+	chr3.1:56369806-56369825	None:intergenic	35.0%
	ACAATGTTGGAACAAATGTG+AGG	-	chr3.1:56370128-56370147	MS.gene63482:CDS	35.0%
	AGACCTTCAGATGATTAACA+AGG	+	chr3.1:56371073-56371092	None:intergenic	35.0%
	ATCACTTCTTATGATCATGC+TGG	+	chr3.1:56370908-56370927	None:intergenic	35.0%
	CAAAAAGTCTTGCTTGTGAA+TGG	-	chr3.1:56370466-56370485	MS.gene63482:intron	35.0%
	CATGATGAGATGCTTGATAA+AGG	-	chr3.1:56371100-56371119	MS.gene63482:CDS	35.0%
	CATTACATATGAAGGGATTG+GGG	-	chr3.1:56369772-56369791	MS.gene63482:intron	35.0%
	CTATAGTGTACACTTGTTCT+AGG	-	chr3.1:56369344-56369363	MS.gene63482:intron	35.0%
	GCATTACATATGAAGGGATT+GGG	-	chr3.1:56369771-56369790	MS.gene63482:intron	35.0%
	TAAGAAGAGGATATGTCAGT+TGG	+	chr3.1:56370231-56370250	None:intergenic	35.0%
	TATCTCGTACACCGATAAAA+CGG	-	chr3.1:56370764-56370783	MS.gene63482:intron	35.0%
	TCACTTCTTATGATCATGCT+GGG	+	chr3.1:56370907-56370926	None:intergenic	35.0%
	TCCGTTGTATACTCAATTGT+TGG	+	chr3.1:56370157-56370176	None:intergenic	35.0%
	TCTCCTTGTTAATCATCTGA+AGG	-	chr3.1:56371067-56371086	MS.gene63482:CDS	35.0%
	TGAGCTTAGGTAAACAATGT+TGG	-	chr3.1:56370115-56370134	MS.gene63482:CDS	35.0%
	TGCATTACATATGAAGGGAT+TGG	-	chr3.1:56369770-56369789	MS.gene63482:intron	35.0%
!	AATAACTTGTCCAGTGATGT+AGG	+	chr3.1:56370848-56370867	None:intergenic	35.0%
!	CTTTTGATGCACTTTGTCTT+TGG	-	chr3.1:56369892-56369911	MS.gene63482:CDS	35.0%
!	GAGAAAGGTTTTTCTGTTCA+TGG	-	chr3.1:56369406-56369425	MS.gene63482:intron	35.0%
!!	CAAGTTATTTCTGTTGATGG+AGG	-	chr3.1:56370859-56370878	MS.gene63482:intron	35.0%
!!	GGACAAGTTATTTCTGTTGA+TGG	-	chr3.1:56370856-56370875	MS.gene63482:intron	35.0%
	GACTTCTTCTTCTCAAAGAG+AGG	-	chr3.1:56369444-56369463	MS.gene63482:intron	40.0%
	GATATGTCAGTTGGCATAGA+TGG	+	chr3.1:56370222-56370241	None:intergenic	40.0%
	GATGATTAACAAGGAGACAC+AGG	+	chr3.1:56371064-56371083	None:intergenic	40.0%
	GATGGAGGATTTACTGCAAA+TGG	-	chr3.1:56370874-56370893	MS.gene63482:intron	40.0%
	GGAAGACACTTCATGTGTTT+CGG	+	chr3.1:56370794-56370813	None:intergenic	40.0%
	TGAATGAACGCTTGAATGAG+TGG	-	chr3.1:56369380-56369399	MS.gene63482:intron	40.0%
	TTGAATGAGTGGAAGGAGAA+AGG	-	chr3.1:56369391-56369410	MS.gene63482:intron	40.0%
	TTGCAAGTGGAAAGCTCAAA+GGG	-	chr3.1:56371161-56371180	MS.gene63482:CDS	40.0%
!	AACACATGAAGTGTCTTCCT+TGG	-	chr3.1:56370795-56370814	MS.gene63482:intron	40.0%
!	AGTGGCGTTTTTGTATACCA+CGG	+	chr3.1:56370525-56370544	None:intergenic	40.0%
!	GTTTCGGCTATCCGTTTTAT+CGG	+	chr3.1:56370778-56370797	None:intergenic	40.0%
!	TGCATAGAGATCCCATTTTC+AGG	+	chr3.1:56369961-56369980	None:intergenic	40.0%
!!	ATCCCATTTTCAGGATTCTG+AGG	+	chr3.1:56369952-56369971	None:intergenic	40.0%
!!	CACTTGTTCTAGGAATGAAG+AGG	-	chr3.1:56369354-56369373	MS.gene63482:intron	40.0%
!!	TCCCATTTTCAGGATTCTGA+GGG	+	chr3.1:56369951-56369970	None:intergenic	40.0%
	AAAGGGTCACATACTCTTGC+TGG	-	chr3.1:56371178-56371197	MS.gene63482:CDS	45.0%
	ACAGTAGCAGAAGCTCAAGA+TGG	-	chr3.1:56369236-56369255	MS.gene63482:CDS	45.0%
	ACCATATACGCAGCGTGTAA+AGG	-	chr3.1:56370319-56370338	MS.gene63482:intron	45.0%
	ACCTTTACACGCTGCGTATA+TGG	+	chr3.1:56370323-56370342	None:intergenic	45.0%
	CAGACAAAGGAAAGCTACCA+AGG	+	chr3.1:56370815-56370834	None:intergenic	45.0%
	CTTGCAAGTGGAAAGCTCAA+AGG	-	chr3.1:56371160-56371179	MS.gene63482:CDS	45.0%
	GCCCTCAGAATCCTGAAAAT+GGG	-	chr3.1:56369947-56369966	MS.gene63482:CDS	45.0%
	GTTGCATCTCTGACAAACGT+AGG	-	chr3.1:56370289-56370308	MS.gene63482:intron	45.0%
	GTTTGTCAGAGATGCAACAG+AGG	+	chr3.1:56370287-56370306	None:intergenic	45.0%
	TCTCTGACAAACGTAGGTTC+TGG	-	chr3.1:56370295-56370314	MS.gene63482:intron	45.0%
	TGAACGCTTGAATGAGTGGA+AGG	-	chr3.1:56369384-56369403	MS.gene63482:intron	45.0%
	TGCAGCTGAAGAAAACAGCA+CGG	+	chr3.1:56370433-56370452	None:intergenic	45.0%
	TGCCCTCAGAATCCTGAAAA+TGG	-	chr3.1:56369946-56369965	MS.gene63482:CDS	45.0%
!	AAGAGAGGAGCTTGTTCGAA+AGG	-	chr3.1:56369459-56369478	MS.gene63482:intron	45.0%
!!	GTAGAGGAACTTGCTGAGTT+TGG	-	chr3.1:56369319-56369338	MS.gene63482:CDS	45.0%
	AAGCTCAAGATGGTCCCTCA+AGG	-	chr3.1:56369246-56369265	MS.gene63482:CDS	50.0%
	AGCTCAAGATGGTCCCTCAA+GGG	-	chr3.1:56369247-56369266	MS.gene63482:CDS	50.0%
	GAGAGCTGTGAATCCCTTGA+GGG	+	chr3.1:56369263-56369282	None:intergenic	50.0%
	GATCGGTCATGCTATCGTAG+AGG	-	chr3.1:56369303-56369322	MS.gene63482:CDS	50.0%
	AGGAAGCAGCTGGCAGACAA+AGG	+	chr3.1:56370828-56370847	None:intergenic	55.0%
	CCAGCTGCTTCCTACATCAC+TGG	-	chr3.1:56370835-56370854	MS.gene63482:intron	55.0%
	CCAGTGATGTAGGAAGCAGC+TGG	+	chr3.1:56370838-56370857	None:intergenic	55.0%
	CGAGAGCTGTGAATCCCTTG+AGG	+	chr3.1:56369264-56369283	None:intergenic	55.0%
	GGATTCACAGCTCTCGTCAC+CGG	-	chr3.1:56369268-56369287	MS.gene63482:CDS	55.0%
!	AGGGATTGGGGTTCTAACGC+CGG	-	chr3.1:56369784-56369803	MS.gene63482:intron	55.0%
	ACCGATCCCACGAGTTCCAC+CGG	+	chr3.1:56369290-56369309	None:intergenic	60.0%
	ACCGGTGGAACTCGTGGGAT+CGG	-	chr3.1:56369286-56369305	MS.gene63482:CDS	60.0%
	GGAGCAACTGTGTTGCACCG+TGG	-	chr3.1:56370505-56370524	MS.gene63482:intron	60.0%
	TCGTCACCGGTGGAACTCGT+GGG	-	chr3.1:56369281-56369300	MS.gene63482:CDS	60.0%
	TTCACAGCTCTCGTCACCGG+TGG	-	chr3.1:56369271-56369290	MS.gene63482:CDS	60.0%
	CTCGTCACCGGTGGAACTCG+TGG	-	chr3.1:56369280-56369299	MS.gene63482:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	56369220	56371207	56369220	ID=MS.gene63482
chr3.1	mRNA	56369220	56371207	56369220	ID=MS.gene63482.t1;Parent=MS.gene63482
chr3.1	exon	56370911	56371207	56370911	ID=MS.gene63482.t1.exon1;Parent=MS.gene63482.t1
chr3.1	CDS	56370911	56371207	56370911	ID=cds.MS.gene63482.t1;Parent=MS.gene63482.t1
chr3.1	exon	56370087	56370303	56370087	ID=MS.gene63482.t1.exon2;Parent=MS.gene63482.t1
chr3.1	CDS	56370087	56370303	56370087	ID=cds.MS.gene63482.t1;Parent=MS.gene63482.t1
chr3.1	exon	56369873	56369979	56369873	ID=MS.gene63482.t1.exon3;Parent=MS.gene63482.t1
chr3.1	CDS	56369873	56369979	56369873	ID=cds.MS.gene63482.t1;Parent=MS.gene63482.t1
chr3.1	exon	56369505	56369697	56369505	ID=MS.gene63482.t1.exon4;Parent=MS.gene63482.t1
chr3.1	CDS	56369505	56369697	56369505	ID=cds.MS.gene63482.t1;Parent=MS.gene63482.t1
chr3.1	exon	56369220	56369338	56369220	ID=MS.gene63482.t1.exon5;Parent=MS.gene63482.t1
chr3.1	CDS	56369220	56369338	56369220	ID=cds.MS.gene63482.t1;Parent=MS.gene63482.t1

Gene Sequence

>MS.gene63482

ATGTCAAAGCAAGAGAACAGTAGCAGAAGCTCAAGATGGTCCCTCAAGGGATTCACAGCTCTCGTCACCGGTGGAACTCGTGGGATCGGTCATGCTATCGTAGAGGAACTTGCTGAGTTTGGTGCTATAGTGTACACTTGTTCTAGGAATGAAGAGGAGCTGAATGAACGCTTGAATGAGTGGAAGGAGAAAGGTTTTTCTGTTCATGGATCAGTTTGTGATGTGACTTCTTCTTCTCAAAGAGAGGAGCTTGTTCGAAAGGTAGCTTCTGATTTCAATGGCAAGCTCAACATACTTGTGAGTTTTTTCTTTTGTATATATTTTGTTTTGCATTTCTAATTTATGTTGATGTTTAAATAGGTCTAAATCAGCTTTTAATTCATGAAAGTGACTCATACAATACATATATTCGTTTTTCAACTTATTGTAGAAAATTTAGTGGAATTTTTTTTTCAGAATATATTTTGATACACGTCCAAATTTCAATCATGTGTAAACGAGTCATTTAAAAAACTCTTAATCGTGTCATGAGCTCAGTTGTTAAGAACATTGCATTACATATGAAGGGATTGGGGTTCTAACGCCGGATTTCCACTTATTCACCTTAAAATGTGAATTTCATGTCTTGTCACACACATTTTAACTTGTAAGTATGCTTAACAAAATTGTAATCTTTTGATGCACTTTGTCTTTGGCCATTTAAGATTTTCTTATGCTTGCTTCAAGTGCCCTCAGAATCCTGAAAATGGGATCTCTATGCATACTCTCTATTTGTATTTAACAATTTTAGCATAAAGCTTAAAACTAGAAATGTTCAAATTGCAAGAATTCTTTTCTGTCACGCATGACTTCAGCTCATACTAAATTGTGCTCATTTCTTAATCTTATATTCTTATGAGCTTAGGTAAACAATGTTGGAACAAATGTGAGGAAACCAACAATTGAGTATACAACGGAAGATTATTCAAAATTGATGAGTATTAACTTGGATTCTGCATACCATCTATGCCAACTGACATATCCTCTTCTTAAAGAATCTGGAAATGGAAGCATTGTATTCAATTCCTCTGTTGCATCTCTGACAAACGTAGGTTCTGGAACCATATACGCAGCGTGTAAAGGTAACTCTATGTATCATTATGTCATTGTATGCTTAAACATGATTGATGGACACAAACTATTTTGACATTTACTAAAGAGATTCGATTCACCGTGCTGTTTTCTTCAGCTGCAATCAATCAGCTGACAAAAAGTCTTGCTTGTGAATGGGCAAAAGACAATATAAGGAGCAACTGTGTTGCACCGTGGTATACAAAAACGCCACTTAACGATTATGTAATGTTCTTTTCATACCTTTAATTATCCATTCAGAGCTCTTATCATTTTTTCTTTATGAAGATTGGTTAAGTTTGTACTGATATAATTTGATTTGGATGAAAAAAGTGAATAATCTTCAAATGACAATGTTATGTTCATAAGCTATGTTGCTAACTGTTTACCTTTCTTACAGTTATATGCTAACAAAGAGTTTGTGAATGAAGTACTATCTCGTACACCGATAAAACGGATAGCCGAAACACATGAAGTGTCTTCCTTGGTAGCTTTCCTTTGTCTGCCAGCTGCTTCCTACATCACTGGACAAGTTATTTCTGTTGATGGAGGATTTACTGCAAATGGATTTCAACCCAGCATGATCATAAGAAGTGATTAATTATGTTTATAAAATAATTCATGACTTATGTATAAGTTTGTTTGAAATTTTATTTTAATGTCTCTCAAAATAAAGCTTATTTTATTGTAAGACCATAGAACATAAGACTATTTCTGCATCATATCATAATCCTGTGTCTCCTTGTTAATCATCTGAAGGTCTATTTCTGCATGATGAGATGCTTGATAAAGGTCTTGTACCTAATGATACAGATACTACATATGATATTCTTGCAAGTGGAAAGCTCAAAGGGTCACATACTCTTGCTGGTGCTTAA

Protein sequence

>MS.gene63482.t1

MSKQENSSRSSRWSLKGFTALVTGGTRGIGHAIVEELAEFGAIVYTCSRNEEELNERLNEWKEKGFSVHGSVCDVTSSSQREELVRKVASDFNGKLNILVNNVGTNVRKPTIEYTTEDYSKLMSINLDSAYHLCQLTYPLLKESGNGSIVFNSSVASLTNVGSGTIYAACKAAINQLTKSLACEWAKDNIRSNCVAPWYTKTPLNDYLYANKEFVNEVLSRTPIKRIAETHEVSSLVAFLCLPAASYITGQVISVDGGFTANGFQPSMIIRSLFLHDEMLDKGLVPNDTDTTYDILASGKLKGSHTLAGA