AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84179


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84179.t1 MTR_7g068770 97.279 294 6 2 1 293 1 293 0.0 575
MS.gene84179.t1 MTR_4g015680 76.984 252 57 1 41 291 28 279 6.21e-119 342
MS.gene84179.t1 MTR_5g026540 46.619 281 123 8 37 292 23 301 1.89e-56 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84179.t1 AT5G01380 55.056 267 96 3 49 291 47 313 1.14e-74 231
MS.gene84179.t1 AT2G38250 57.299 274 96 4 33 291 19 286 3.17e-74 229
MS.gene84179.t1 AT3G25990 31.858 113 74 1 54 163 55 167 6.03e-14 71.6
MS.gene84179.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.10e-11 64.3
MS.gene84179.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.10e-11 64.3
MS.gene84179.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.89e-11 64.3
MS.gene84179.t1 AT1G13450 33.696 92 61 0 54 145 87 178 2.98e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAAACTCCTTTGTTTCT+TGG 0.162176 7.1:+33040239 None:intergenic
AGAACTAGATCAAACTTTCA+TGG 0.325286 7.1:-33040206 MS.gene84179:CDS
GCTTGAAGCTCATTGTAAAA+TGG 0.335464 7.1:+33039528 None:intergenic
AAACCTTGTTACTCGCTATA+AGG 0.349897 7.1:-33040086 MS.gene84179:intron
GTTGATGTTGTTGATGATGT+TGG 0.399503 7.1:+33040389 None:intergenic
TGAGAGGAGATTGAAGGAGA+TGG 0.403012 7.1:-33039217 MS.gene84179:CDS
CAAAGAGGAACAAACAGCTT+TGG 0.409902 7.1:-33040180 MS.gene84179:CDS
AAAGAGGAACAAACAGCTTT+GGG 0.427051 7.1:-33040179 MS.gene84179:CDS
CTCTAACACTATGAAGGAAA+AGG 0.429380 7.1:-33040149 MS.gene84179:CDS
CAACATGCTCATAACATCAT+AGG 0.432593 7.1:-33040313 MS.gene84179:CDS
TGGAACAAAGATGGAGAGAA+AGG 0.438127 7.1:-33039149 MS.gene84179:CDS
GGAGATTGAAGGAGATGGAA+TGG 0.449920 7.1:-33039212 MS.gene84179:CDS
AGAGAATGAGAGGAGATTGA+AGG 0.459157 7.1:-33039223 MS.gene84179:CDS
GAAGGATTAGAGAGGAAGCT+AGG 0.463838 7.1:-33039116 MS.gene84179:CDS
TCTAACACTATGAAGGAAAA+GGG 0.470472 7.1:-33040148 MS.gene84179:CDS
AGATTCTTGAGGAGTTTATG+AGG 0.475642 7.1:-33039293 MS.gene84179:CDS
TCAATGGATGGAAGCATTTG+AGG 0.478413 7.1:-33039250 MS.gene84179:CDS
TCTGAGGATGAAGAAGAGTT+AGG 0.481694 7.1:-33039426 MS.gene84179:CDS
AAGGAGGGTCAAAGAAGAAA+GGG 0.488338 7.1:-33039461 MS.gene84179:CDS
AAATGCAAATGGAAGCTCAA+TGG 0.497271 7.1:-33039266 MS.gene84179:CDS
GATGGAATGGAGACAAAGAA+TGG 0.497428 7.1:-33039199 MS.gene84179:CDS
TATGAGGCAACAAATGCAAA+TGG 0.498234 7.1:-33039277 MS.gene84179:CDS
GGTTAATGATGGAACAAAGA+TGG 0.500019 7.1:-33039158 MS.gene84179:CDS
GTTAGGCAATGAAGAAAGTG+AGG 0.500463 7.1:-33039409 MS.gene84179:CDS
GAGACAAAGAATGGAAGCAT+TGG 0.501965 7.1:-33039190 MS.gene84179:CDS
GAAGGAGGGTCAAAGAAGAA+AGG 0.513151 7.1:-33039462 MS.gene84179:CDS
AAAGGTAAAATGATAATTGG+TGG 0.519046 7.1:-33039351 MS.gene84179:CDS
CAAGGATGCAAAGAATGCTA+TGG 0.520600 7.1:-33039494 MS.gene84179:CDS
GAGAAAGGGAAGAACAAAGA+AGG 0.520747 7.1:-33039134 MS.gene84179:CDS
GGAACAAAGATGGAGAGAAA+GGG 0.522554 7.1:-33039148 MS.gene84179:CDS
TAGTGGTAGCAACAATTTGA+AGG 0.523300 7.1:-33039316 MS.gene84179:CDS
AGTTATCTCTAACACTATGA+AGG 0.525230 7.1:-33040155 MS.gene84179:CDS
TCACGGATAGATTTCCTCAA+TGG 0.529342 7.1:-33040267 MS.gene84179:CDS
GGTGGAGGAAATGATAATAG+TGG 0.531218 7.1:-33039333 MS.gene84179:CDS
TGGAAGCATTGGAGAATGAG+AGG 0.532379 7.1:-33039179 MS.gene84179:CDS
GGAGAATGAGAGGTTAATGA+TGG 0.535043 7.1:-33039169 MS.gene84179:CDS
CAGAACAGTGCAAGTGCAAG+TGG 0.545159 7.1:-33040111 MS.gene84179:CDS
CAATTTGAAGGAGATTCTTG+AGG 0.551112 7.1:-33039304 MS.gene84179:CDS
GGTAAAATGATAATTGGTGG+AGG 0.559022 7.1:-33039348 MS.gene84179:CDS
AAATGGAAATTGTTGTCTCA+TGG 0.560448 7.1:+33039545 None:intergenic
AAGGATTAGAGAGGAAGCTA+GGG 0.562239 7.1:-33039115 MS.gene84179:CDS
TAGTGTTAACGTTGATGTCA+CGG 0.564347 7.1:-33040284 MS.gene84179:CDS
GTTTCTTGGATACTCCATTG+AGG 0.570470 7.1:+33040253 None:intergenic
ATGCTATGGGCTGAAGCAGA+AGG 0.573045 7.1:-33039480 MS.gene84179:CDS
GCAAATGGAAGCTCAATGGA+TGG 0.584747 7.1:-33039262 MS.gene84179:CDS
AGAAAGTGAGGGAGATCACA+AGG 0.594638 7.1:-33039397 MS.gene84179:CDS
TTAGGCAATGAAGAAAGTGA+GGG 0.597635 7.1:-33039408 MS.gene84179:CDS
CTATGGGCTGAAGCAGAAGG+AGG 0.599474 7.1:-33039477 MS.gene84179:CDS
TATAAAGGGATGTGAAACAA+TGG 0.603946 7.1:-33039577 MS.gene84179:intron
AAACTTTCATGGAGACAAAG+AGG 0.604350 7.1:-33040195 MS.gene84179:CDS
TTGAGGCAAGAGAGAATGAG+AGG 0.604352 7.1:-33039233 MS.gene84179:CDS
AGAACAAAGAAGGATTAGAG+AGG 0.604574 7.1:-33039124 MS.gene84179:CDS
ATGGAGTATCCAAGAAACAA+AGG 0.617542 7.1:-33040248 MS.gene84179:CDS
AGGGGTGCATGTATCTTCTG+AGG 0.631436 7.1:-33039442 MS.gene84179:CDS
AAGTTTGATCTAGTTCTGAA+CGG 0.639250 7.1:+33040212 None:intergenic
AAGGATGCAAAGAATGCTAT+GGG 0.642173 7.1:-33039493 MS.gene84179:CDS
GTACCTTATAGCGAGTAACA+AGG 0.656453 7.1:+33040083 None:intergenic
AGGAGGGTCAAAGAAGAAAG+GGG 0.670389 7.1:-33039460 MS.gene84179:CDS
TATGGGCTGAAGCAGAAGGA+GGG 0.673793 7.1:-33039476 MS.gene84179:CDS
GAAAGTGAGGGAGATCACAA+GGG 0.730407 7.1:-33039396 MS.gene84179:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTAAACTTTTTTTT+TGG - chr7.1:33039586-33039605 MS.gene84179:intron 10.0%
!! AAAAAAGGTAAAATGATAAT+TGG - chr7.1:33040105-33040124 MS.gene84179:CDS 15.0%
!! ATTAAAAAGAAGAAGAAAAA+AGG - chr7.1:33040090-33040109 MS.gene84179:CDS 15.0%
!!! TTATGTTTTTGTGATATAAA+GGG - chr7.1:33039868-33039887 MS.gene84179:intron 15.0%
!!! TTTATGTTTTTGTGATATAA+AGG - chr7.1:33039867-33039886 MS.gene84179:intron 15.0%
!! AAAAGGCAATAAATAAAAAG+TGG + chr7.1:33039664-33039683 None:intergenic 20.0%
!! AAACAAGAATGATTTATGAT+TGG + chr7.1:33039508-33039527 None:intergenic 20.0%
!! CACATAGAATTATTAATGAT+TGG + chr7.1:33039449-33039468 None:intergenic 20.0%
! AAAGGTAAAATGATAATTGG+TGG - chr7.1:33040108-33040127 MS.gene84179:CDS 25.0%
!! TTTATATTGTGAGCTTTTCA+TGG - chr7.1:33039803-33039822 MS.gene84179:intron 25.0%
!!! ATTGCCTTTTCAATTTGTTA+GGG - chr7.1:33039674-33039693 MS.gene84179:intron 25.0%
!!! GTTAAACTTTTTTTTTGGAC+AGG - chr7.1:33039591-33039610 MS.gene84179:intron 25.0%
!!! TATTGCCTTTTCAATTTGTT+AGG - chr7.1:33039673-33039692 MS.gene84179:intron 25.0%
AAATGGAAATTGTTGTCTCA+TGG + chr7.1:33039917-33039936 None:intergenic 30.0%
AGAACTAGATCAAACTTTCA+TGG - chr7.1:33039253-33039272 MS.gene84179:CDS 30.0%
AGTTATCTCTAACACTATGA+AGG - chr7.1:33039304-33039323 MS.gene84179:CDS 30.0%
TATAAAGGGATGTGAAACAA+TGG - chr7.1:33039882-33039901 MS.gene84179:intron 30.0%
TCTAACACTATGAAGGAAAA+GGG - chr7.1:33039311-33039330 MS.gene84179:CDS 30.0%
TTTCTCTCTCATGAATCAAA+GGG - chr7.1:33039705-33039724 MS.gene84179:intron 30.0%
! TAAGAAACTCCTTTGTTTCT+TGG + chr7.1:33039223-33039242 None:intergenic 30.0%
! TTTTCTCTCTCATGAATCAA+AGG - chr7.1:33039704-33039723 MS.gene84179:intron 30.0%
!! AAGTTTGATCTAGTTCTGAA+CGG + chr7.1:33039250-33039269 None:intergenic 30.0%
!!! TTCAAGCTATTTTTAGTGCA+AGG - chr7.1:33039947-33039966 MS.gene84179:intron 30.0%
!!! TTGCCTTTTCAATTTGTTAG+GGG - chr7.1:33039675-33039694 MS.gene84179:intron 30.0%
AAACCTTGTTACTCGCTATA+AGG - chr7.1:33039373-33039392 MS.gene84179:CDS 35.0%
AAACTTTCATGGAGACAAAG+AGG - chr7.1:33039264-33039283 MS.gene84179:CDS 35.0%
AAAGAGGAACAAACAGCTTT+GGG - chr7.1:33039280-33039299 MS.gene84179:CDS 35.0%
AAATGCAAATGGAAGCTCAA+TGG - chr7.1:33040193-33040212 MS.gene84179:CDS 35.0%
AAGGATGCAAAGAATGCTAT+GGG - chr7.1:33039966-33039985 MS.gene84179:intron 35.0%
AGAACAAAGAAGGATTAGAG+AGG - chr7.1:33040335-33040354 MS.gene84179:CDS 35.0%
AGATTCTTGAGGAGTTTATG+AGG - chr7.1:33040166-33040185 MS.gene84179:CDS 35.0%
CAACATGCTCATAACATCAT+AGG - chr7.1:33039146-33039165 MS.gene84179:CDS 35.0%
CAATTTGAAGGAGATTCTTG+AGG - chr7.1:33040155-33040174 MS.gene84179:CDS 35.0%
CTCTAACACTATGAAGGAAA+AGG - chr7.1:33039310-33039329 MS.gene84179:CDS 35.0%
GCTTGAAGCTCATTGTAAAA+TGG + chr7.1:33039934-33039953 None:intergenic 35.0%
GGTAAAATGATAATTGGTGG+AGG - chr7.1:33040111-33040130 MS.gene84179:CDS 35.0%
TACCCCCTAACAAATTGAAA+AGG + chr7.1:33039681-33039700 None:intergenic 35.0%
TAGTGGTAGCAACAATTTGA+AGG - chr7.1:33040143-33040162 MS.gene84179:CDS 35.0%
TAGTGTTAACGTTGATGTCA+CGG - chr7.1:33039175-33039194 MS.gene84179:CDS 35.0%
TATGAGGCAACAAATGCAAA+TGG - chr7.1:33040182-33040201 MS.gene84179:CDS 35.0%
TTAGGCAATGAAGAAAGTGA+GGG - chr7.1:33040051-33040070 MS.gene84179:intron 35.0%
! ATGGAGTATCCAAGAAACAA+AGG - chr7.1:33039211-33039230 MS.gene84179:CDS 35.0%
! GGTTAATGATGGAACAAAGA+TGG - chr7.1:33040301-33040320 MS.gene84179:CDS 35.0%
!! GTTGATGTTGTTGATGATGT+TGG + chr7.1:33039073-33039092 None:intergenic 35.0%
!!! TGCCTTTTCAATTTGTTAGG+GGG - chr7.1:33039676-33039695 MS.gene84179:intron 35.0%
AAGGAGGGTCAAAGAAGAAA+GGG - chr7.1:33039998-33040017 MS.gene84179:intron 40.0%
AAGGATTAGAGAGGAAGCTA+GGG - chr7.1:33040344-33040363 MS.gene84179:CDS 40.0%
AGAGAATGAGAGGAGATTGA+AGG - chr7.1:33040236-33040255 MS.gene84179:CDS 40.0%
CAAAGAGGAACAAACAGCTT+TGG - chr7.1:33039279-33039298 MS.gene84179:CDS 40.0%
CAAGGATGCAAAGAATGCTA+TGG - chr7.1:33039965-33039984 MS.gene84179:intron 40.0%
GAGAAAGGGAAGAACAAAGA+AGG - chr7.1:33040325-33040344 MS.gene84179:CDS 40.0%
GATGGAATGGAGACAAAGAA+TGG - chr7.1:33040260-33040279 MS.gene84179:CDS 40.0%
GGAACAAAGATGGAGAGAAA+GGG - chr7.1:33040311-33040330 MS.gene84179:CDS 40.0%
GGAGAATGAGAGGTTAATGA+TGG - chr7.1:33040290-33040309 MS.gene84179:CDS 40.0%
GGTGGAGGAAATGATAATAG+TGG - chr7.1:33040126-33040145 MS.gene84179:CDS 40.0%
GTACCTTATAGCGAGTAACA+AGG + chr7.1:33039379-33039398 None:intergenic 40.0%
GTTAGGCAATGAAGAAAGTG+AGG - chr7.1:33040050-33040069 MS.gene84179:intron 40.0%
GTTTCTTGGATACTCCATTG+AGG + chr7.1:33039209-33039228 None:intergenic 40.0%
TCACGGATAGATTTCCTCAA+TGG - chr7.1:33039192-33039211 MS.gene84179:CDS 40.0%
TCTGAGGATGAAGAAGAGTT+AGG - chr7.1:33040033-33040052 MS.gene84179:intron 40.0%
TGGAACAAAGATGGAGAGAA+AGG - chr7.1:33040310-33040329 MS.gene84179:CDS 40.0%
! GAGACAAAGAATGGAAGCAT+TGG - chr7.1:33040269-33040288 MS.gene84179:CDS 40.0%
!! TCAATGGATGGAAGCATTTG+AGG - chr7.1:33040209-33040228 MS.gene84179:CDS 40.0%
AGAAAGTGAGGGAGATCACA+AGG - chr7.1:33040062-33040081 MS.gene84179:intron 45.0%
AGGAGGGTCAAAGAAGAAAG+GGG - chr7.1:33039999-33040018 MS.gene84179:intron 45.0%
GAAAGTGAGGGAGATCACAA+GGG - chr7.1:33040063-33040082 MS.gene84179:intron 45.0%
GAAGGAGGGTCAAAGAAGAA+AGG - chr7.1:33039997-33040016 MS.gene84179:intron 45.0%
GAAGGATTAGAGAGGAAGCT+AGG - chr7.1:33040343-33040362 MS.gene84179:CDS 45.0%
GCAAATGGAAGCTCAATGGA+TGG - chr7.1:33040197-33040216 MS.gene84179:CDS 45.0%
GGAGATTGAAGGAGATGGAA+TGG - chr7.1:33040247-33040266 MS.gene84179:CDS 45.0%
TGAGAGGAGATTGAAGGAGA+TGG - chr7.1:33040242-33040261 MS.gene84179:CDS 45.0%
TTGAGGCAAGAGAGAATGAG+AGG - chr7.1:33040226-33040245 MS.gene84179:CDS 45.0%
!! TGGAAGCATTGGAGAATGAG+AGG - chr7.1:33040280-33040299 MS.gene84179:CDS 45.0%
CAGAACAGTGCAAGTGCAAG+TGG - chr7.1:33039348-33039367 MS.gene84179:CDS 50.0%
! AGGGGTGCATGTATCTTCTG+AGG - chr7.1:33040017-33040036 MS.gene84179:intron 50.0%
! ATGCTATGGGCTGAAGCAGA+AGG - chr7.1:33039979-33039998 MS.gene84179:intron 50.0%
! TATGGGCTGAAGCAGAAGGA+GGG - chr7.1:33039983-33040002 MS.gene84179:intron 50.0%
! CTATGGGCTGAAGCAGAAGG+AGG - chr7.1:33039982-33040001 MS.gene84179:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 33039053 33040428 33039053 ID=MS.gene84179
chr7.1 mRNA 33039053 33040428 33039053 ID=MS.gene84179.t1;Parent=MS.gene84179
chr7.1 exon 33040087 33040428 33040087 ID=MS.gene84179.t1.exon1;Parent=MS.gene84179.t1
chr7.1 CDS 33040087 33040428 33040087 ID=cds.MS.gene84179.t1;Parent=MS.gene84179.t1
chr7.1 exon 33039053 33039592 33039053 ID=MS.gene84179.t1.exon2;Parent=MS.gene84179.t1
chr7.1 CDS 33039053 33039592 33039053 ID=cds.MS.gene84179.t1;Parent=MS.gene84179.t1
Gene Sequence

>MS.gene84179

ATGGAGGGTCATCATCTCCAACATCATCAACAACATCAACAACAACATCATCAACAACATCAACAGCAACATCAACAAAGACAACATCAACAACAACATGCTCATAACATCATAGGTACTAGTAGTGTTAACGTTGATGTCACGGATAGATTTCCTCAATGGAGTATCCAAGAAACAAAGGAGTTTCTTATGATCCGTTCAGAACTAGATCAAACTTTCATGGAGACAAAGAGGAACAAACAGCTTTGGGAAGTTATCTCTAACACTATGAAGGAAAAGGGTTATCATAGAAGTGCAGAACAGTGCAAGTGCAAGTGGAAAAACCTTGTTACTCGCTATAAGGTACTTCTATTCATTCATATATACATATATAAAAAAGAATTACACTATCAACCAATCATTAATAATTCTATGTGCATAATAATAAAATCTTTCATATAATATATTACTAACCAATCATAAATCATTCTTGTTTCAATAAATTTGCTTATAAAGAAATCTAATTAAAATTATTGCAATTAAGCAAATCATGTATTTTGTTAAACTTTTTTTTTGGACAGGTTTTTGTTAATCTTATACACATACATAGATATATATACATACATATGCCACTTTTTATTTATTGCCTTTTCAATTTGTTAGGGGGTAAACTTTTCTCTCTCATGAATCAAAGGGTACTTTTAAAAAAAAATAAAAGTTCATATTTAGTTCTATATATTAATATGTAACACTTTAACTGAATATATGATTTTTATATTGTGAGCTTTTCATGGCTGTTTGTTATATATATATGTATAGTAAAACTATTGTTTTGTTTATGTTTTTGTGATATAAAGGGATGTGAAACAATGGAGCTAGAAGCCATGAGACAACAATTTCCATTTTACAATGAGCTTCAAGCTATTTTTAGTGCAAGGATGCAAAGAATGCTATGGGCTGAAGCAGAAGGAGGGTCAAAGAAGAAAGGGGTGCATGTATCTTCTGAGGATGAAGAAGAGTTAGGCAATGAAGAAAGTGAGGGAGATCACAAGGGCAACATTAAAAAGAAGAAGAAAAAAGGTAAAATGATAATTGGTGGAGGAAATGATAATAGTGGTAGCAACAATTTGAAGGAGATTCTTGAGGAGTTTATGAGGCAACAAATGCAAATGGAAGCTCAATGGATGGAAGCATTTGAGGCAAGAGAGAATGAGAGGAGATTGAAGGAGATGGAATGGAGACAAAGAATGGAAGCATTGGAGAATGAGAGGTTAATGATGGAACAAAGATGGAGAGAAAGGGAAGAACAAAGAAGGATTAGAGAGGAAGCTAGGGCTGAAAAGAGAGATGAATTAATCACTGCTTTGCTTGATAAGCTTACAAGAGAAGACATGTAG

Protein sequence

>MS.gene84179.t1

MEGHHLQHHQQHQQQHHQQHQQQHQQRQHQQQHAHNIIGTSSVNVDVTDRFPQWSIQETKEFLMIRSELDQTFMETKRNKQLWEVISNTMKEKGYHRSAEQCKCKWKNLVTRYKGCETMELEAMRQQFPFYNELQAIFSARMQRMLWAEAEGGSKKKGVHVSSEDEEELGNEESEGDHKGNIKKKKKKGKMIIGGGNDNSGSNNLKEILEEFMRQQMQMEAQWMEAFEARENERRLKEMEWRQRMEALENERLMMEQRWREREEQRRIREEARAEKRDELITALLDKLTREDM