AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85298


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85298.t1 MTR_2g045080 91.228 171 5 1 1 161 1 171 1.02e-112 317
MS.gene85298.t1 MTR_2g045050 77.019 161 37 0 1 161 1 161 4.32e-93 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85298.t1 AT1G48320 63.462 156 55 1 6 161 3 156 1.30e-69 207
MS.gene85298.t1 AT5G48950 54.667 150 65 2 11 157 3 152 5.44e-58 178
MS.gene85298.t1 AT5G48950 60.185 108 41 1 11 116 3 110 4.35e-43 139

Find 20 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTGTTGCCAAAACTATTC+AGG 0.263280 2.4:-45351772 MS.gene85298:intron
GATTTACCCCTTCATACATT+TGG 0.265244 2.4:-45352926 MS.gene85298:CDS
GTGTCATCAAACAAAGTTTC+TGG 0.297801 2.4:-45352887 MS.gene85298:CDS
AAAACCAAAAGCTGCTGATT+TGG 0.309085 2.4:-45352948 MS.gene85298:CDS
CCTCCATGAAGCACACTAAA+AGG 0.309265 2.4:+45351942 None:intergenic
AAATCCAAATCAGCAGCTTT+TGG 0.333199 2.4:+45352944 None:intergenic
ATTTACCCCTTCATACATTT+GGG 0.334094 2.4:-45352925 MS.gene85298:CDS
TGCATACTTCTTTAGCATAT+TGG 0.339467 2.4:+45351115 None:intergenic
AGCATGCACCTGAATAGTTT+TGG 0.351762 2.4:+45351764 None:intergenic
TGTGGGTATAAAAGAGTTGC+TGG 0.445165 2.4:-45351867 MS.gene85298:CDS
GCTGAAGCTCTTGCAAGTAT+TGG 0.487992 2.4:-45351903 MS.gene85298:CDS
AGGTGTGGGATGTGAAAATC+TGG 0.507075 2.4:-45351238 MS.gene85298:CDS
ACTCAAACCCAAATGTATGA+AGG 0.525757 2.4:+45352918 None:intergenic
TTGGAGCACATGTTGCTTGT+GGG 0.528993 2.4:-45351884 MS.gene85298:CDS
CTCAAACCCAAATGTATGAA+GGG 0.530132 2.4:+45352919 None:intergenic
CATTTGAAATCAGCAATGCT+TGG 0.536764 2.4:-45351825 MS.gene85298:CDS
AGTCACTGAAAAGTGTTGCC+AGG 0.553880 2.4:-45352855 MS.gene85298:intron
GGAGGACCATCAATCATCAT+CGG 0.605735 2.4:-45352972 MS.gene85298:CDS
ATTGGAGCACATGTTGCTTG+TGG 0.616187 2.4:-45351885 MS.gene85298:CDS
TCAAACCCAAATGTATGAAG+GGG 0.634737 2.4:+45352920 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGAGAATTTATATAAA+TGG + chr2.4:45352474-45352493 None:intergenic 10.0%
!! AATAATTGTTATAAGAATTA+AGG + chr2.4:45351720-45351739 None:intergenic 10.0%
!! ATAATAAAATAAATGATAGT+AGG - chr2.4:45351490-45351509 MS.gene85298:intron 10.0%
!! ATAATTGTTATAAGAATTAA+GGG + chr2.4:45351719-45351738 None:intergenic 10.0%
!! ATTTATATAAATTCTCTTAT+TGG - chr2.4:45352473-45352492 MS.gene85298:intron 10.0%
!!! ATATCATCTCATATTTATTT+TGG - chr2.4:45352564-45352583 MS.gene85298:intron 15.0%
!! AAAAATTTCAACACAACATT+CGG + chr2.4:45352762-45352781 None:intergenic 20.0%
!! ACGAAAAATTATGAAAACAA+AGG + chr2.4:45351257-45351276 None:intergenic 20.0%
!! ATAAATGATAGTAGGTATTA+TGG - chr2.4:45351498-45351517 MS.gene85298:intron 20.0%
!! ATATATGAACAAGAAAAAGT+AGG - chr2.4:45352082-45352101 MS.gene85298:intron 20.0%
!! ATCATGATTAGTATAATCAT+TGG + chr2.4:45352647-45352666 None:intergenic 20.0%
!! CGAAAAATTATGAAAACAAA+GGG + chr2.4:45351256-45351275 None:intergenic 20.0%
!! CGAATTCTGATTTAAATAAT+TGG + chr2.4:45352369-45352388 None:intergenic 20.0%
!! TTCTGATTTAAATAATTGGT+CGG + chr2.4:45352365-45352384 None:intergenic 20.0%
!!! ACTTTGAGAAAAATATAAAG+AGG + chr2.4:45352342-45352361 None:intergenic 20.0%
!!! TTACTTGCTTACATTTTTTT+GGG - chr2.4:45352780-45352799 MS.gene85298:intron 20.0%
!!! TTTACTTGCTTACATTTTTT+TGG - chr2.4:45352779-45352798 MS.gene85298:intron 20.0%
! AATTAGGAGTGATTTGATAT+TGG - chr2.4:45352106-45352125 MS.gene85298:intron 25.0%
! ACAAGAAAAAGTAGGTAATT+AGG - chr2.4:45352090-45352109 MS.gene85298:intron 25.0%
! CACATAAAGATAAATCGTTT+GGG + chr2.4:45352404-45352423 None:intergenic 25.0%
! TCACATAAAGATAAATCGTT+TGG + chr2.4:45352405-45352424 None:intergenic 25.0%
! TTGAAGACAATATCAGTAAT+TGG - chr2.4:45352439-45352458 MS.gene85298:intron 25.0%
! TTGAGTATGTTACATAGTTA+TGG - chr2.4:45352026-45352045 MS.gene85298:intron 25.0%
!! TCATAATTTTTCGTCTCTTA+GGG - chr2.4:45351263-45351282 MS.gene85298:intron 25.0%
!! TTCATAATTTTTCGTCTCTT+AGG - chr2.4:45351262-45351281 MS.gene85298:intron 25.0%
!!! CTCATATTTATTTTGGTACT+TGG - chr2.4:45352571-45352590 MS.gene85298:intron 25.0%
!!! TACTTGCTTACATTTTTTTG+GGG - chr2.4:45352781-45352800 MS.gene85298:intron 25.0%
AAACATGAAGGAAAGATTGA+AGG + chr2.4:45351678-45351697 None:intergenic 30.0%
AATTAAGCACATATACTCCT+TGG - chr2.4:45351760-45351779 MS.gene85298:intron 30.0%
ACAATATCAGTAATTGGCTA+TGG - chr2.4:45352445-45352464 MS.gene85298:intron 30.0%
ATTTACCCCTTCATACATTT+GGG - chr2.4:45351155-45351174 MS.gene85298:CDS 30.0%
CTCAAGTCATTAATGAACTT+CGG + chr2.4:45352704-45352723 None:intergenic 30.0%
GACAATGCATAAAATATGCA+AGG - chr2.4:45351831-45351850 MS.gene85298:CDS 30.0%
GTAAGAGGAAAGATTGTTAA+AGG - chr2.4:45351323-45351342 MS.gene85298:intron 30.0%
TAGTGAAAGTGAATAGAAAG+TGG - chr2.4:45351404-45351423 MS.gene85298:intron 30.0%
TGCATACTTCTTTAGCATAT+TGG + chr2.4:45352968-45352987 None:intergenic 30.0%
! ATAAGAATTAAGGGTGTGTT+TGG + chr2.4:45351710-45351729 None:intergenic 30.0%
! GTTAAAGGAAAGAAAGTATG+AGG - chr2.4:45351338-45351357 MS.gene85298:intron 30.0%
!!! ATAGTTTTGGCAACACTTAA+AGG + chr2.4:45352306-45352325 None:intergenic 30.0%
!!! TAGTTTTGGCAACACTTAAA+GGG + chr2.4:45352305-45352324 None:intergenic 30.0%
AAAGAGACATGATCATGTGT+TGG - chr2.4:45351882-45351901 MS.gene85298:CDS 35.0%
AAAGATTGAAGGTGGAAAGA+AGG + chr2.4:45351667-45351686 None:intergenic 35.0%
ACATGATCATGTCTAATCCA+AGG + chr2.4:45351780-45351799 None:intergenic 35.0%
ACTCAAACCCAAATGTATGA+AGG + chr2.4:45351165-45351184 None:intergenic 35.0%
ATCTTTCCTCTCACTTTCTT+TGG + chr2.4:45351302-45351321 None:intergenic 35.0%
CTCAAACCCAAATGTATGAA+GGG + chr2.4:45351164-45351183 None:intergenic 35.0%
GAAAGTGAATAGAAAGTGGA+AGG - chr2.4:45351408-45351427 MS.gene85298:intron 35.0%
GATTTACCCCTTCATACATT+TGG - chr2.4:45351154-45351173 MS.gene85298:CDS 35.0%
GTGTCATCAAACAAAGTTTC+TGG - chr2.4:45351193-45351212 MS.gene85298:CDS 35.0%
TCAAACCCAAATGTATGAAG+GGG + chr2.4:45351163-45351182 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+CGG - chr2.4:45351836-45351855 MS.gene85298:CDS 35.0%
! AAAACCAAAAGCTGCTGATT+TGG - chr2.4:45351132-45351151 MS.gene85298:CDS 35.0%
! AAATCCAAATCAGCAGCTTT+TGG + chr2.4:45351139-45351158 None:intergenic 35.0%
! AAGTGTTGCCAAAACTATTC+AGG - chr2.4:45352308-45352327 MS.gene85298:intron 35.0%
! AATTAAGGGTGTGTTTGGTT+GGG + chr2.4:45351705-45351724 None:intergenic 35.0%
! ACTTTTCAGTGACTTGAAGA+TGG + chr2.4:45351219-45351238 None:intergenic 35.0%
! ATGATCATGTCTCTTTTTGG+TGG + chr2.4:45351878-45351897 None:intergenic 35.0%
! CACATGATCATGTCTCTTTT+TGG + chr2.4:45351881-45351900 None:intergenic 35.0%
! CATTTGAAATCAGCAATGCT+TGG - chr2.4:45352255-45352274 MS.gene85298:intron 35.0%
! GAAAGTATGAGGAAAGTGAA+TGG - chr2.4:45351349-45351368 MS.gene85298:intron 35.0%
! GTTCTGTTTTGTGAGTTTGA+AGG + chr2.4:45352877-45352896 None:intergenic 35.0%
! TGAGCTTAGTTCAGTTGATA+TGG - chr2.4:45351809-45351828 MS.gene85298:CDS 35.0%
!! AGTTTTGGCAACACTTAAAG+GGG + chr2.4:45352304-45352323 None:intergenic 35.0%
!! ATTTTGGTACTTGGTTCCAA+GGG - chr2.4:45352580-45352599 MS.gene85298:intron 35.0%
!! GGAAAGTGAGATGATTTGTT+AGG - chr2.4:45351370-45351389 MS.gene85298:intron 35.0%
!! TCTTTTGCATAATCTGGAAC+AGG + chr2.4:45352940-45352959 None:intergenic 35.0%
!!! TATTTTGGTACTTGGTTCCA+AGG - chr2.4:45352579-45352598 MS.gene85298:intron 35.0%
!!! TGCTTACATTTTTTTGGGGT+TGG - chr2.4:45352785-45352804 MS.gene85298:intron 35.0%
AAAATGTTTCCGCACACCAT+AGG + chr2.4:45351563-45351582 None:intergenic 40.0%
AACAGCCTAAGAAAGAGAAG+TGG + chr2.4:45351614-45351633 None:intergenic 40.0%
ACACGAACACCAACAAAACA+CGG + chr2.4:45352006-45352025 None:intergenic 40.0%
CATAAAATATGCAAGGTCCG+GGG - chr2.4:45351838-45351857 MS.gene85298:CDS 40.0%
CATGAAGGAAAGATTGAAGG+TGG + chr2.4:45351675-45351694 None:intergenic 40.0%
GATATGTGTCAGTATCACGT+CGG + chr2.4:45351927-45351946 None:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG - chr2.4:45351837-45351856 MS.gene85298:CDS 40.0%
GTGAGAGGAAAGATTGTAAG+AGG - chr2.4:45351308-45351327 MS.gene85298:intron 40.0%
TCCATCCACTTCTCTTTCTT+AGG - chr2.4:45351606-45351625 MS.gene85298:intron 40.0%
TGAAAACAAAGGGTTTGACC+TGG + chr2.4:45351246-45351265 None:intergenic 40.0%
TGTGGGTATAAAAGAGTTGC+TGG - chr2.4:45352213-45352232 MS.gene85298:intron 40.0%
TTACACTTTGATCCTGTTGC+AGG - chr2.4:45352822-45352841 MS.gene85298:intron 40.0%
TTGAAGGTGGAAAGAAGGAA+CGG + chr2.4:45351662-45351681 None:intergenic 40.0%
! AGCATGCACCTGAATAGTTT+TGG + chr2.4:45352319-45352338 None:intergenic 40.0%
! ATGGTGTGCGGAAACATTTT+TGG - chr2.4:45351563-45351582 MS.gene85298:intron 40.0%
! CTTTTAGTGTGCTTCATGGA+GGG - chr2.4:45352139-45352158 MS.gene85298:intron 40.0%
! GAATTAAGGGTGTGTTTGGT+TGG + chr2.4:45351706-45351725 None:intergenic 40.0%
! GCAGCTTCTTTTGCATAATC+TGG + chr2.4:45352946-45352965 None:intergenic 40.0%
! GTTTTGCCGATGATGATTGA+TGG + chr2.4:45351117-45351136 None:intergenic 40.0%
! TGTCTATGTCCGTGTTTTGT+TGG - chr2.4:45351994-45352013 MS.gene85298:intron 40.0%
! TTTTAGTGTGCTTCATGGAG+GGG - chr2.4:45352140-45352159 MS.gene85298:intron 40.0%
!! AAGTGGATGGATTGTTTGCA+TGG + chr2.4:45351597-45351616 None:intergenic 40.0%
!! AGTGGATGGATTGTTTGCAT+GGG + chr2.4:45351596-45351615 None:intergenic 40.0%
!! ATCATGTCTCTTTTTGGTGG+TGG + chr2.4:45351875-45351894 None:intergenic 40.0%
ACTTTCTGTCCTATGGTGTG+CGG - chr2.4:45351551-45351570 MS.gene85298:intron 45.0%
AGGTGTGGGATGTGAAAATC+TGG - chr2.4:45352842-45352861 MS.gene85298:intron 45.0%
CACACCATAGGACAGAAAGT+GGG + chr2.4:45351551-45351570 None:intergenic 45.0%
CCTCCATGAAGCACACTAAA+AGG + chr2.4:45352141-45352160 None:intergenic 45.0%
GCCTAAGAAAGAGAAGTGGA+TGG + chr2.4:45351610-45351629 None:intergenic 45.0%
GCTGAAGCTCTTGCAAGTAT+TGG - chr2.4:45352177-45352196 MS.gene85298:intron 45.0%
GGAGGACCATCAATCATCAT+CGG - chr2.4:45351108-45351127 MS.gene85298:CDS 45.0%
GGATCACCAAAGAAAGTGAG+AGG - chr2.4:45351293-45351312 MS.gene85298:intron 45.0%
TCAACTGAACTAAGCTCACG+AGG + chr2.4:45351807-45351826 None:intergenic 45.0%
TCCAAACTGAGGAAGGATGT+TGG - chr2.4:45351633-45351652 MS.gene85298:intron 45.0%
TCCAACATCCTTCCTCAGTT+TGG + chr2.4:45351637-45351656 None:intergenic 45.0%
TCTTAGGCTGTTCCAAACTG+AGG - chr2.4:45351622-45351641 MS.gene85298:intron 45.0%
TTCGTCTCTTAGGGTGTGTT+TGG - chr2.4:45351272-45351291 MS.gene85298:intron 45.0%
TTTGATCCTGTTGCAGGTGT+GGG - chr2.4:45352828-45352847 MS.gene85298:intron 45.0%
! ATTGGAGCACATGTTGCTTG+TGG - chr2.4:45352195-45352214 MS.gene85298:intron 45.0%
! CAGCCTTTTAGTGTGCTTCA+TGG - chr2.4:45352135-45352154 MS.gene85298:intron 45.0%
! CCTTTTAGTGTGCTTCATGG+AGG - chr2.4:45352138-45352157 MS.gene85298:intron 45.0%
! TGGTTGGGCTAGAAACATGA+AGG + chr2.4:45351690-45351709 None:intergenic 45.0%
! TTGGAGCACATGTTGCTTGT+GGG - chr2.4:45352196-45352215 MS.gene85298:intron 45.0%
!! AGTCACTGAAAAGTGTTGCC+AGG - chr2.4:45351225-45351244 MS.gene85298:CDS 45.0%
!!! TGCGGAAACATTTTTGGCGT+GGG - chr2.4:45351569-45351588 MS.gene85298:intron 45.0%
!! ATAAAATAATACTTATAAAA+GGG + chr2.4:45351469-45351488 None:intergenic 5.0%
!! TATAAAATAATACTTATAAA+AGG + chr2.4:45351470-45351489 None:intergenic 5.0%
AGGCTGTTCCAAACTGAGGA+AGG - chr2.4:45351626-45351645 MS.gene85298:intron 50.0%
CTTTGATCCTGTTGCAGGTG+TGG - chr2.4:45352827-45352846 MS.gene85298:intron 50.0%
GCACACCATAGGACAGAAAG+TGG + chr2.4:45351552-45351571 None:intergenic 50.0%
TCAGCCCACTTTCTGTCCTA+TGG - chr2.4:45351544-45351563 MS.gene85298:intron 50.0%
TGGTACTTGGTTCCAAGGGT+TGG - chr2.4:45352584-45352603 MS.gene85298:intron 50.0%
!!! GTCTCTTTTTGGTGGTGGCA+TGG + chr2.4:45351870-45351889 None:intergenic 50.0%
!!! GTGCGGAAACATTTTTGGCG+TGG - chr2.4:45351568-45351587 MS.gene85298:intron 50.0%
GAACATCGAGCTCCAACCCT+TGG + chr2.4:45352599-45352618 None:intergenic 55.0%
TCACATCCCACACCTGCAAC+AGG + chr2.4:45352837-45352856 None:intergenic 55.0%
TGGTGGCATGGTTCGAACCC+CGG + chr2.4:45351858-45351877 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 45351106 45352996 45351106 ID=MS.gene85298
chr2.4 mRNA 45351106 45352996 45351106 ID=MS.gene85298.t1;Parent=MS.gene85298
chr2.4 exon 45352856 45352996 45352856 ID=MS.gene85298.t1.exon1;Parent=MS.gene85298.t1
chr2.4 CDS 45352856 45352996 45352856 ID=cds.MS.gene85298.t1;Parent=MS.gene85298.t1
chr2.4 exon 45351773 45351964 45351773 ID=MS.gene85298.t1.exon2;Parent=MS.gene85298.t1
chr2.4 CDS 45351773 45351964 45351773 ID=cds.MS.gene85298.t1;Parent=MS.gene85298.t1
chr2.4 exon 45351106 45351258 45351106 ID=MS.gene85298.t1.exon3;Parent=MS.gene85298.t1
chr2.4 CDS 45351106 45351258 45351106 ID=cds.MS.gene85298.t1;Parent=MS.gene85298.t1
Gene Sequence

>MS.gene85298

ATGGAGGACCATCAATCATCATCGGCAAAACCAAAAGCTGCTGATTTGGATTTACCCCTTCATACATTTGGGTTTGAGTTTCAAGATGTGTCATCAAACAAAGTTTCTGGCCATCTTCAAGTCACTGAAAAGTGTTGCCAGGTCAAACCCTTTGTTTTCATAATTTTTCGTCTCTTAGGGTGTGTTTGGATCACCAAAGAAAGTGAGAGGAAAGATTGTAAGAGGAAAGATTGTTAAAGGAAAGAAAGTATGAGGAAAGTGAATGGAAAGTGAGATGATTTGTTAGGTTGTTTGATTATAGTGAAAGTGAATAGAAAGTGGAAGGAAAAAATAAACTATATTTTGTAAATGACATTTATACCCTTTTATAAGTATTATTTTATAATAATAAAATAAATGATAGTAGGTATTATGGTCTTTTCACTCAAAAAGTAACAATCAGCCCACTTTCTGTCCTATGGTGTGCGGAAACATTTTTGGCGTGGGTCCCATGCAAACAATCCATCCACTTCTCTTTCTTAGGCTGTTCCAAACTGAGGAAGGATGTTGGAATCCGTTCCTTCTTTCCACCTTCAATCTTTCCTTCATGTTTCTAGCCCAACCAAACACACCCTTAATTCTTATAACAATTATTTGATACTATGATTCTTGAAAAATTAAGCACATATACTCCTTGGATTAGACATGATCATGTGTGTCCTCGTGAGCTTAGTTCAGTTGATATGGACAATGCATAAAATATGCAAGGTCCGGGGTTCGAACCATGCCACCACCAAAAAGAGACATGATCATGTGTTGGATATGTAACATGTCTGACACCGACGTGATACTGACACATATCATTATATTGAATTATGTTATTTTCTCAAATTATTATCAGCTTTGACGTGTCTATGTCCGTGTTTTGTTGGTGTTCGTGTTTGAGTATGTTACATAGTTATGGAATCATATTATTTTTTATATTAATTTCTCACATATATATGAACAAGAAAAAGTAGGTAATTAGGAGTGATTTGATATTGGATGTTGCAGCCTTTTAGTGTGCTTCATGGAGGGGTATCAGCAGTGATAGCTGAAGCTCTTGCAAGTATTGGAGCACATGTTGCTTGTGGGTATAAAAGAGTTGCTGGAATTCAACTTAGCATCAATCATTTGAAATCAGCAATGCTTGGTGACTTGATCTATGCTGAAGCAACCCCTTTAAGTGTTGCCAAAACTATTCAGGTGCATGCTCCTCTTTATATTTTTCTCAAAGTCCGACCAATTATTTAAATCAGAATTCGTACTCAAGTTTTCCCAAACGATTTATCTTTATGTGAAGCAGACTAAATAATTGAAGACAATATCAGTAATTGGCTATGGTTACCATTTATATAAATTCTCTTATTGGTGATTTGATGCATATTAACAACAACAGAACATAAGACATACTCAAAGATGTTTTTGTAATTTTAATAGATATCATCTCATATTTATTTTGGTACTTGGTTCCAAGGGTTGGAGCTCGATGTTCGACTGTAAAGTAAGTAAAAACGAGACCAATGATTATACTAATCATGATTCGAGTTCAGCTCTCTTAAACAATTTGTCTTTAACCGAAGTTCATTAATGACTTGAGTATAATTGTTTGTTTGAATCATCTCATTGTTTACTCCGAATGTTGTGTTGAAATTTTTACTTGCTTACATTTTTTTGGGGTTGGATATAATCTTTATCTTACACTTTGATCCTGTTGCAGGTGTGGGATGTGAAAATCTGGAAGATCGATCCTTCAAACTCACAAAACAGAACGTTGATAGCATATTCTAGAGTAACTCTTAAAAGCAACATGCCTGTTCCAGATTATGCAAAAGAAGCTGCCAATATGCTAAAGAAGTATGCAAAACTGTAA

Protein sequence

>MS.gene85298.t1

MEDHQSSSAKPKAADLDLPLHTFGFEFQDVSSNKVSGHLQVTEKCCQPFSVLHGGVSAVIAEALASIGAHVACGYKRVAGIQLSINHLKSAMLGDLIYAEATPLSVAKTIQVWDVKIWKIDPSNSQNRTLIAYSRVTLKSNMPVPDYAKEAANMLKKYAKL