Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98848.t1 | KEH27042.1 | 93.7 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 3.70E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98848.t1 | A0A072UBQ9 | 93.7 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 2.7e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene98848 | 0.817781 | 2.76E-52 | -1.69E-46 |
| MS.gene049178 | MS.gene98848 | 0.826575 | 2.52E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene98848 | 0.809765 | 1.61E-50 | -1.69E-46 |
| MS.gene049567 | MS.gene98848 | 0.814458 | 1.52E-51 | -1.69E-46 |
| MS.gene049647 | MS.gene98848 | 0.816899 | 4.36E-52 | -1.69E-46 |
| MS.gene049958 | MS.gene98848 | 0.812482 | 4.15E-51 | -1.69E-46 |
| MS.gene050137 | MS.gene98848 | 0.814984 | 1.17E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene98848 | 0.800197 | 1.61E-48 | -1.69E-46 |
| MS.gene050226 | MS.gene98848 | 0.804055 | 2.60E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene98848 | 0.821617 | 3.67E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene98848 | 0.815943 | 7.13E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene98848 | 0.809686 | 1.67E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene98848 | 0.80382 | 2.90E-49 | -1.69E-46 |
| MS.gene050438 | MS.gene98848 | 0.815816 | 7.62E-52 | -1.69E-46 |
| MS.gene050439 | MS.gene98848 | 0.807545 | 4.80E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene98848 | 0.841545 | 4.45E-58 | -1.69E-46 |
| MS.gene050719 | MS.gene98848 | 0.854323 | 1.33E-61 | -1.69E-46 |
| MS.gene050721 | MS.gene98848 | 0.852585 | 4.19E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene98848 | 0.831131 | 1.99E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene98848 | 0.88444 | 1.91E-71 | -1.69E-46 |
| MS.gene050815 | MS.gene98848 | 0.812523 | 4.06E-51 | -1.69E-46 |
| MS.gene050826 | MS.gene98848 | 0.810866 | 9.31E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene98848 | 0.82998 | 3.80E-55 | -1.69E-46 |
| MS.gene050953 | MS.gene98848 | 0.802475 | 5.51E-49 | -1.69E-46 |
| MS.gene051207 | MS.gene98848 | 0.826192 | 3.10E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene98848 | 0.849895 | 2.41E-60 | -1.69E-46 |
| MS.gene051265 | MS.gene98848 | 0.81696 | 4.22E-52 | -1.69E-46 |
| MS.gene051388 | MS.gene98848 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene051490 | MS.gene98848 | 0.834032 | 3.79E-56 | -1.69E-46 |
| MS.gene051492 | MS.gene98848 | 0.813842 | 2.09E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene98848 | 0.803235 | 3.84E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene98848 | 0.816412 | 5.60E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene98848 | 0.829122 | 6.14E-55 | -1.69E-46 |
| MS.gene051790 | MS.gene98848 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene98848 | 0.827092 | 1.89E-54 | -1.69E-46 |
| MS.gene052020 | MS.gene98848 | 0.829292 | 5.59E-55 | -1.69E-46 |
| MS.gene052586 | MS.gene98848 | 0.806425 | 8.28E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene98848 | 0.81723 | 3.67E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene98848 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene98848 | 0.842422 | 2.61E-58 | -1.69E-46 |
| MS.gene053147 | MS.gene98848 | 0.81448 | 1.51E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene98848 | 0.812068 | 5.11E-51 | -1.69E-46 |
| MS.gene053641 | MS.gene98848 | 0.852194 | 5.42E-61 | -1.69E-46 |
| MS.gene053647 | MS.gene98848 | 0.806499 | 7.98E-50 | -1.69E-46 |
| MS.gene053811 | MS.gene98848 | 0.813748 | 2.19E-51 | -1.69E-46 |
| MS.gene053966 | MS.gene98848 | 0.800161 | 1.64E-48 | -1.69E-46 |
| MS.gene05410 | MS.gene98848 | 0.809738 | 1.63E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene98848 | 0.845336 | 4.33E-59 | -1.69E-46 |
| MS.gene05415 | MS.gene98848 | 0.811203 | 7.87E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene98848 | 0.836788 | 7.62E-57 | -1.69E-46 |
| MS.gene05427 | MS.gene98848 | 0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene98848 | -0.808547 | 2.94E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene98848 | 0.818519 | 1.88E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene98848 | 0.847132 | 1.40E-59 | -1.69E-46 |
| MS.gene054472 | MS.gene98848 | 0.825075 | 5.71E-54 | -1.69E-46 |
| MS.gene054576 | MS.gene98848 | 0.827582 | 1.45E-54 | -1.69E-46 |
| MS.gene054581 | MS.gene98848 | 0.802579 | 5.25E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene98848 | 0.911398 | 6.36E-83 | -1.69E-46 |
| MS.gene054775 | MS.gene98848 | 0.854094 | 1.55E-61 | -1.69E-46 |
| MS.gene055313 | MS.gene98848 | 0.824579 | 7.48E-54 | -1.69E-46 |
| MS.gene055357 | MS.gene98848 | 0.821879 | 3.19E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene98848 | 0.815582 | 8.59E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene98848 | 0.815842 | 7.52E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene98848 | 0.801946 | 7.08E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene98848 | 0.800074 | 1.71E-48 | -1.69E-46 |
| MS.gene055608 | MS.gene98848 | 0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene98848 | 0.82741 | 1.59E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene98848 | 0.820929 | 5.29E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene98848 | 0.850632 | 1.50E-60 | -1.69E-46 |
| MS.gene05604 | MS.gene98848 | 0.815748 | 7.88E-52 | -1.69E-46 |
| MS.gene056373 | MS.gene98848 | 0.80955 | 1.79E-50 | -1.69E-46 |
| MS.gene056552 | MS.gene98848 | 0.80141 | 9.13E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene98848 | 0.839838 | 1.25E-57 | -1.69E-46 |
| MS.gene057360 | MS.gene98848 | 0.800577 | 1.35E-48 | -1.69E-46 |
| MS.gene057693 | MS.gene98848 | 0.828937 | 6.81E-55 | -1.69E-46 |
| MS.gene057736 | MS.gene98848 | 0.80578 | 1.13E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene98848 | 0.836239 | 1.05E-56 | -1.69E-46 |
| MS.gene058084 | MS.gene98848 | 0.810633 | 1.05E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene98848 | 0.808915 | 2.45E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene98848 | 0.835552 | 1.57E-56 | -1.69E-46 |
| MS.gene058477 | MS.gene98848 | 0.811174 | 7.99E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene98848 | 0.831417 | 1.69E-55 | -1.69E-46 |
| MS.gene058906 | MS.gene98848 | 0.809417 | 1.91E-50 | -1.69E-46 |
| MS.gene059084 | MS.gene98848 | 0.803668 | 3.12E-49 | -1.69E-46 |
| MS.gene059220 | MS.gene98848 | 0.80126 | 9.79E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene98848 | 0.811965 | 5.38E-51 | -1.69E-46 |
| MS.gene059664 | MS.gene98848 | 0.807631 | 4.60E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene98848 | 0.818309 | 2.10E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene98848 | 0.838244 | 3.23E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene98848 | 0.812705 | 3.71E-51 | -1.69E-46 |
| MS.gene060637 | MS.gene98848 | 0.806592 | 7.63E-50 | -1.69E-46 |
| MS.gene060781 | MS.gene98848 | 0.802789 | 4.75E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene98848 | 0.824093 | 9.73E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene98848 | 0.843883 | 1.07E-58 | -1.69E-46 |
| MS.gene060998 | MS.gene98848 | 0.829123 | 6.14E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene98848 | -0.816334 | 5.83E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98848.t1 | MTR_6g084590 | 93.671 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 9.51e-46 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGAGAGGTGGTGATTATA+AGG | 0.344076 | 6.1:+74061812 | MS.gene98848:CDS |
| CCACCACTTTCAGCAGCTTC+CGG | 0.372406 | 6.1:-74061862 | None:intergenic |
| ATTAAGAAGAAGGAAAAGAT+TGG | 0.429526 | 6.1:+74061790 | MS.gene98848:CDS |
| AAGGAAAAGGTGGAAGAGAA+TGG | 0.465732 | 6.1:+74061683 | MS.gene98848:CDS |
| CTGACGACCGCGAGCTCCGC+CGG | 0.466025 | 6.1:-74061724 | None:intergenic |
| TCGTCGCTGACGACCGCGGC+CGG | 0.473574 | 6.1:+74061761 | MS.gene98848:CDS |
| GAGGGAAACTCTTGAGAAAC+CGG | 0.490287 | 6.1:+74061705 | MS.gene98848:CDS |
| GGAAAAGATTGGAGGAGAGG+TGG | 0.510074 | 6.1:+74061801 | MS.gene98848:CDS |
| TTTGATGACAAAGGAAAAGG+TGG | 0.521585 | 6.1:+74061673 | MS.gene98848:CDS |
| CGTCGCTGACGACCGCGGCC+GGG | 0.522259 | 6.1:+74061762 | MS.gene98848:CDS |
| CCGGGAAAATATTAAGAAGA+AGG | 0.522927 | 6.1:+74061780 | MS.gene98848:CDS |
| AAAAGGTGGAAGAGAATGGA+GGG | 0.528096 | 6.1:+74061687 | MS.gene98848:CDS |
| GAAGGAAAAGATTGGAGGAG+AGG | 0.529761 | 6.1:+74061798 | MS.gene98848:CDS |
| AAGAAGAAGGAAAAGATTGG+AGG | 0.538627 | 6.1:+74061793 | MS.gene98848:CDS |
| TGAGAAACCGGCGGAGCTCG+CGG | 0.545036 | 6.1:+74061717 | MS.gene98848:CDS |
| GAAAAGGTGGAAGAGAATGG+AGG | 0.573388 | 6.1:+74061686 | MS.gene98848:CDS |
| GCACCGGAAGCTGCTGAAAG+TGG | 0.573462 | 6.1:+74061859 | MS.gene98848:CDS |
| CCGGAAGCTGCTGAAAGTGG+TGG | 0.590260 | 6.1:+74061862 | MS.gene98848:CDS |
| TGATTTGAATGATGTGGCAC+CGG | 0.610457 | 6.1:+74061843 | MS.gene98848:CDS |
| GATATCGTCGCTGACGACCG+CGG | 0.676789 | 6.1:+74061757 | MS.gene98848:CDS |
| GGAAACTCTTGAGAAACCGG+CGG | 0.715924 | 6.1:+74061708 | MS.gene98848:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAAATATTTTGATGACAA+AGG | + | chr6.1:74061664-74061683 | MS.gene98848:CDS | 20.0% |
| ! | ATTAAGAAGAAGGAAAAGAT+TGG | + | chr6.1:74061790-74061809 | MS.gene98848:CDS | 25.0% |
| !!! | TATTTTGATGACAAAGGAAA+AGG | + | chr6.1:74061670-74061689 | MS.gene98848:CDS | 25.0% |
| ! | CCTTCTTCTTAATATTTTCC+CGG | - | chr6.1:74061783-74061802 | None:intergenic | 30.0% |
| !!! | AGCTTTTGATTTGAATGATG+TGG | + | chr6.1:74061837-74061856 | MS.gene98848:CDS | 30.0% |
| AAGAAGAAGGAAAAGATTGG+AGG | + | chr6.1:74061793-74061812 | MS.gene98848:CDS | 35.0% | |
| CCGGGAAAATATTAAGAAGA+AGG | + | chr6.1:74061780-74061799 | MS.gene98848:CDS | 35.0% | |
| ! | TTTGATGACAAAGGAAAAGG+TGG | + | chr6.1:74061673-74061692 | MS.gene98848:CDS | 35.0% |
| AAAAGGTGGAAGAGAATGGA+GGG | + | chr6.1:74061687-74061706 | MS.gene98848:CDS | 40.0% | |
| AAGGAAAAGGTGGAAGAGAA+TGG | + | chr6.1:74061683-74061702 | MS.gene98848:CDS | 40.0% | |
| TGATTTGAATGATGTGGCAC+CGG | + | chr6.1:74061843-74061862 | MS.gene98848:CDS | 40.0% | |
| GAAAAGGTGGAAGAGAATGG+AGG | + | chr6.1:74061686-74061705 | MS.gene98848:CDS | 45.0% | |
| GAAGGAAAAGATTGGAGGAG+AGG | + | chr6.1:74061798-74061817 | MS.gene98848:CDS | 45.0% | |
| GAGGAGAGGTGGTGATTATA+AGG | + | chr6.1:74061812-74061831 | MS.gene98848:CDS | 45.0% | |
| GAGGGAAACTCTTGAGAAAC+CGG | + | chr6.1:74061705-74061724 | MS.gene98848:CDS | 45.0% | |
| ! | TCTTAATATTTTCCCGGCCG+CGG | - | chr6.1:74061777-74061796 | None:intergenic | 45.0% |
| GGAAAAGATTGGAGGAGAGG+TGG | + | chr6.1:74061801-74061820 | MS.gene98848:CDS | 50.0% | |
| GGAAACTCTTGAGAAACCGG+CGG | + | chr6.1:74061708-74061727 | MS.gene98848:CDS | 50.0% | |
| CCACCACTTTCAGCAGCTTC+CGG | - | chr6.1:74061865-74061884 | None:intergenic | 55.0% | |
| CCGGAAGCTGCTGAAAGTGG+TGG | + | chr6.1:74061862-74061881 | MS.gene98848:CDS | 60.0% | |
| GATATCGTCGCTGACGACCG+CGG | + | chr6.1:74061757-74061776 | MS.gene98848:CDS | 60.0% | |
| GCACCGGAAGCTGCTGAAAG+TGG | + | chr6.1:74061859-74061878 | MS.gene98848:CDS | 60.0% | |
| TGAGAAACCGGCGGAGCTCG+CGG | + | chr6.1:74061717-74061736 | MS.gene98848:CDS | 65.0% | |
| CTGACGACCGCGAGCTCCGC+CGG | - | chr6.1:74061727-74061746 | None:intergenic | 75.0% | |
| TCGTCGCTGACGACCGCGGC+CGG | + | chr6.1:74061761-74061780 | MS.gene98848:CDS | 75.0% | |
| ! | CGTCGCTGACGACCGCGGCC+GGG | + | chr6.1:74061762-74061781 | MS.gene98848:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 74061652 | 74061891 | 74061652 | ID=MS.gene98848 |
| chr6.1 | mRNA | 74061652 | 74061891 | 74061652 | ID=MS.gene98848.t1;Parent=MS.gene98848 |
| chr6.1 | exon | 74061652 | 74061891 | 74061652 | ID=MS.gene98848.t1.exon1;Parent=MS.gene98848.t1 |
| chr6.1 | CDS | 74061652 | 74061891 | 74061652 | ID=cds.MS.gene98848.t1;Parent=MS.gene98848.t1 |
| Gene Sequence |
| Protein sequence |
