AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010448.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010448.01.T01 MTR_2g084425 96.460 113 4 0 1 113 1 113 8.82e-67 217
MsG0280010448.01.T01 MTR_2g084425 96.460 113 4 0 1 113 1 113 1.11e-66 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010448.01.T01 AT2G27980 39.869 153 61 7 1 125 1 150 4.59e-16 73.6

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCGATTCAAATGAGTTTA+TGG 0.161340 2:-69797972 MsG0280010448.01.T01:CDS
ATGAGTTTATGGTTCTTTCT+AGG 0.201514 2:-69797961 MsG0280010448.01.T01:CDS
AAGAATGTTGAAAGGGAAAA+TGG 0.205543 2:-69797713 MsG0280010448.01.T01:CDS
CCGTAATGAGGGTCCGGTTC+TGG 0.288512 2:-69797843 MsG0280010448.01.T01:CDS
TTTCTTCTTCTTCCTTCAAA+AGG 0.351014 2:+69797636 None:intergenic
CCAACCGGCGTTTCCAGAAC+CGG 0.376249 2:+69797830 None:intergenic
AGAGTTTGCGTTTGCAATGA+AGG 0.408420 2:-69797918 MsG0280010448.01.T01:CDS
TCTGAGATCGATGGTTCGTT+GGG 0.410716 2:-69797890 MsG0280010448.01.T01:CDS
CCAGAACCGGACCCTCATTA+CGG 0.422256 2:+69797843 None:intergenic
TGGAAACGCCGGTTGGGAAA+AGG 0.425455 2:-69797823 MsG0280010448.01.T01:CDS
TTCTTCTTCTTCCTTCAAAA+GGG 0.425646 2:+69797637 None:intergenic
AGAATGTTGAAAGGGAAAAT+GGG 0.428531 2:-69797712 MsG0280010448.01.T01:CDS
AACCATAAACTCATTTGAAT+CGG 0.438511 2:+69797970 None:intergenic
TGAGTTTATGGTTCTTTCTA+GGG 0.447578 2:-69797960 MsG0280010448.01.T01:CDS
CGGTTCTGGAAACGCCGGTT+GGG 0.454316 2:-69797829 MsG0280010448.01.T01:CDS
ATCTGAGATCGATGGTTCGT+TGG 0.456099 2:-69797891 MsG0280010448.01.T01:CDS
GTTCTTTCTAGGGTTAGAAC+AGG 0.463394 2:-69797950 MsG0280010448.01.T01:CDS
GGAAACGCCGGTTGGGAAAA+GGG 0.480645 2:-69797822 MsG0280010448.01.T01:CDS
ATGGTTCGTTGGGTCGAACC+AGG 0.484401 2:-69797880 MsG0280010448.01.T01:CDS
ACGGTTCTTACTACCACCCC+TGG 0.500779 2:+69797862 None:intergenic
CCGGTTCTGGAAACGCCGGT+TGG 0.511153 2:-69797830 MsG0280010448.01.T01:CDS
CATAACAACATCATCCAAAC+CGG 0.512225 2:+69797790 None:intergenic
TAAGAACCGTAATGAGGGTC+CGG 0.518478 2:-69797849 MsG0280010448.01.T01:CDS
AAGAGGTTAAGAATGTTGAA+AGG 0.522341 2:-69797721 MsG0280010448.01.T01:CDS
AGAGGTTAAGAATGTTGAAA+GGG 0.531488 2:-69797720 MsG0280010448.01.T01:CDS
TGGTTCGTTGGGTCGAACCA+GGG 0.550602 2:-69797879 MsG0280010448.01.T01:CDS
TGGTAGTAAGAACCGTAATG+AGG 0.555759 2:-69797855 MsG0280010448.01.T01:CDS
GGTTCGTTGGGTCGAACCAG+GGG 0.564985 2:-69797878 MsG0280010448.01.T01:CDS
TATAATCATTCTATGGCGAA+AGG 0.574673 2:-69797995 None:intergenic
GGATCTTGTTAGCGATGAAG+AGG 0.578002 2:-69797738 MsG0280010448.01.T01:CDS
AGAGGCTAAGAGTGATGTTG+TGG 0.581325 2:-69797759 MsG0280010448.01.T01:CDS
GGATGATGTTGTTATGAGTG+AGG 0.582454 2:-69797783 MsG0280010448.01.T01:CDS
TGTTGTTATGAGTGAGGAAG+AGG 0.584412 2:-69797777 MsG0280010448.01.T01:CDS
TCGTTGGGTCGAACCAGGGG+TGG 0.590035 2:-69797875 MsG0280010448.01.T01:CDS
GGTAGTAAGAACCGTAATGA+GGG 0.606032 2:-69797854 MsG0280010448.01.T01:CDS
AAGGCTCAATCTGAGATCGA+TGG 0.644296 2:-69797899 MsG0280010448.01.T01:CDS
GGGTCCGGTTCTGGAAACGC+CGG 0.690311 2:-69797834 MsG0280010448.01.T01:CDS
TATTGATGTGAAGAGTGATG+TGG 0.737875 2:-69797672 MsG0280010448.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACCATAAACTCATTTGAAT+CGG + Chr2:69797644-69797663 None:intergenic 25.0%
AAGAATGTTGAAAGGGAAAA+TGG - Chr2:69797898-69797917 MsG0280010448.01.T01:CDS 30.0%
AAGAGGTTAAGAATGTTGAA+AGG - Chr2:69797890-69797909 MsG0280010448.01.T01:CDS 30.0%
AGAATGTTGAAAGGGAAAAT+GGG - Chr2:69797899-69797918 MsG0280010448.01.T01:CDS 30.0%
AGAGGTTAAGAATGTTGAAA+GGG - Chr2:69797891-69797910 MsG0280010448.01.T01:CDS 30.0%
ATGAGTTTATGGTTCTTTCT+AGG - Chr2:69797650-69797669 MsG0280010448.01.T01:CDS 30.0%
TGAGTTTATGGTTCTTTCTA+GGG - Chr2:69797651-69797670 MsG0280010448.01.T01:CDS 30.0%
TTCTTCTTCTTCCTTCAAAA+GGG + Chr2:69797977-69797996 None:intergenic 30.0%
TTTCTTCTTCTTCCTTCAAA+AGG + Chr2:69797978-69797997 None:intergenic 30.0%
! AACCGATTCAAATGAGTTTA+TGG - Chr2:69797639-69797658 MsG0280010448.01.T01:CDS 30.0%
AAGGGTAAAAAAAACCGGTT+TGG - Chr2:69797807-69797826 MsG0280010448.01.T01:CDS 35.0%
CATAACAACATCATCCAAAC+CGG + Chr2:69797824-69797843 None:intergenic 35.0%
GGGAAAAGGGTAAAAAAAAC+CGG - Chr2:69797802-69797821 MsG0280010448.01.T01:CDS 35.0%
TATTGATGTGAAGAGTGATG+TGG - Chr2:69797939-69797958 MsG0280010448.01.T01:CDS 35.0%
! TGATGAAGAAACCCTTTTGA+AGG - Chr2:69797963-69797982 MsG0280010448.01.T01:CDS 35.0%
!!! TTTTTTACCCTTTTCCCAAC+CGG + Chr2:69797799-69797818 None:intergenic 35.0%
AGAGTTTGCGTTTGCAATGA+AGG - Chr2:69797693-69797712 MsG0280010448.01.T01:CDS 40.0%
GGATGATGTTGTTATGAGTG+AGG - Chr2:69797828-69797847 MsG0280010448.01.T01:CDS 40.0%
GGTAGTAAGAACCGTAATGA+GGG - Chr2:69797757-69797776 MsG0280010448.01.T01:CDS 40.0%
TGGTAGTAAGAACCGTAATG+AGG - Chr2:69797756-69797775 MsG0280010448.01.T01:CDS 40.0%
TGTTGTTATGAGTGAGGAAG+AGG - Chr2:69797834-69797853 MsG0280010448.01.T01:CDS 40.0%
!! GTTCTTTCTAGGGTTAGAAC+AGG - Chr2:69797661-69797680 MsG0280010448.01.T01:CDS 40.0%
AGAGGCTAAGAGTGATGTTG+TGG - Chr2:69797852-69797871 MsG0280010448.01.T01:CDS 45.0%
GGATCTTGTTAGCGATGAAG+AGG - Chr2:69797873-69797892 MsG0280010448.01.T01:CDS 45.0%
TAAGAACCGTAATGAGGGTC+CGG - Chr2:69797762-69797781 MsG0280010448.01.T01:CDS 45.0%
! AAGGCTCAATCTGAGATCGA+TGG - Chr2:69797712-69797731 MsG0280010448.01.T01:CDS 45.0%
! ATCTGAGATCGATGGTTCGT+TGG - Chr2:69797720-69797739 MsG0280010448.01.T01:CDS 45.0%
! TCTGAGATCGATGGTTCGTT+GGG - Chr2:69797721-69797740 MsG0280010448.01.T01:CDS 45.0%
ACGGTTCTTACTACCACCCC+TGG + Chr2:69797752-69797771 None:intergenic 55.0%
ATGGTTCGTTGGGTCGAACC+AGG - Chr2:69797731-69797750 MsG0280010448.01.T01:CDS 55.0%
CCAGAACCGGACCCTCATTA+CGG + Chr2:69797771-69797790 None:intergenic 55.0%
TGGTTCGTTGGGTCGAACCA+GGG - Chr2:69797732-69797751 MsG0280010448.01.T01:CDS 55.0%
! GGAAACGCCGGTTGGGAAAA+GGG - Chr2:69797789-69797808 MsG0280010448.01.T01:CDS 55.0%
! TGGAAACGCCGGTTGGGAAA+AGG - Chr2:69797788-69797807 MsG0280010448.01.T01:CDS 55.0%
CCAACCGGCGTTTCCAGAAC+CGG + Chr2:69797784-69797803 None:intergenic 60.0%
CCGTAATGAGGGTCCGGTTC+TGG - Chr2:69797768-69797787 MsG0280010448.01.T01:CDS 60.0%
GGTTCGTTGGGTCGAACCAG+GGG - Chr2:69797733-69797752 MsG0280010448.01.T01:CDS 60.0%
!! CGGTTCTGGAAACGCCGGTT+GGG - Chr2:69797782-69797801 MsG0280010448.01.T01:CDS 60.0%
TCGTTGGGTCGAACCAGGGG+TGG - Chr2:69797736-69797755 MsG0280010448.01.T01:CDS 65.0%
!! CCGGTTCTGGAAACGCCGGT+TGG - Chr2:69797781-69797800 MsG0280010448.01.T01:CDS 65.0%
!! GGGTCCGGTTCTGGAAACGC+CGG - Chr2:69797777-69797796 MsG0280010448.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 69797628 69798005 69797628 ID=MsG0280010448.01;Name=MsG0280010448.01
Chr2 mRNA 69797628 69798005 69797628 ID=MsG0280010448.01.T01;Parent=MsG0280010448.01;Name=MsG0280010448.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|125
Chr2 exon 69797628 69798005 69797628 ID=MsG0280010448.01.T01:exon:9876;Parent=MsG0280010448.01.T01
Chr2 CDS 69797628 69798005 69797628 ID=MsG0280010448.01.T01:cds;Parent=MsG0280010448.01.T01
Gene Sequence

>MsG0280010448.01.T01

ATGGCGAAAGGAACCGATTCAAATGAGTTTATGGTTCTTTCTAGGGTTAGAACAGGTTTGAAACGAGAGTTTGCGTTTGCAATGAAGGCTCAATCTGAGATCGATGGTTCGTTGGGTCGAACCAGGGGTGGTAGTAAGAACCGTAATGAGGGTCCGGTTCTGGAAACGCCGGTTGGGAAAAGGGTAAAAAAAACCGGTTTGGATGATGTTGTTATGAGTGAGGAAGAGGCTAAGAGTGATGTTGTGGATCTTGTTAGCGATGAAGAGGTTAAGAATGTTGAAAGGGAAAATGGGTTTTTGTCAAAATCTGATATTGATGTGAAGAGTGATGTGGTTGATGAAGAAACCCTTTTGAAGGAAGAAGAAGAAAAGTTGTGA

Protein sequence

>MsG0280010448.01.T01

MAKGTDSNEFMVLSRVRTGLKREFAFAMKAQSEIDGSLGRTRGGSKNRNEGPVLETPVGKRVKKTGLDDVVMSEEEAKSDVVDLVSDEEVKNVERENGFLSKSDIDVKSDVVDEETLLKEEEEKL*