AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032996.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032996.01.T01 MTR_8g037235 61.333 75 29 0 1 75 940 1014 2.22e-20 85.1
MsG0680032996.01.T01 MTR_8g037235 58.333 72 22 1 1 72 988 1051 6.11e-16 72.4
MsG0680032996.01.T01 MTR_8g037235 67.391 46 15 0 30 75 921 966 1.07e-12 63.2
MsG0680032996.01.T01 MTR_8g012005 54.286 70 32 0 1 70 150 219 4.95e-16 71.6
MsG0680032996.01.T01 MTR_8g011870 48.649 74 35 1 5 75 211 284 3.67e-12 61.6
MsG0680032996.01.T01 MTR_8g011870 48.000 75 23 1 1 75 258 316 5.88e-12 60.8
MsG0680032996.01.T01 MTR_3g072140 67.391 46 13 1 30 75 827 870 8.28e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTACGGGTAAACGCAAAA+TGG 0.192242 6:-54405826 MsG0680032996.01.T01:CDS
CATGCAGGCGTCACCTAAAC+TGG 0.324518 6:-54405880 MsG0680032996.01.T01:CDS
GATGATTGCACATCCACTTT+AGG 0.339367 6:+54406035 None:intergenic
ACTACGGGTAAACGCAAAAT+GGG 0.399612 6:-54405825 MsG0680032996.01.T01:CDS
TTCTCATAAATGTCCAGTTT+AGG 0.408958 6:+54405867 None:intergenic
GACGCCTGCATGTCTACTTT+GGG 0.436436 6:+54405891 None:intergenic
TGACGCCTGCATGTCTACTT+TGG 0.441500 6:+54405890 None:intergenic
ATCATCGCATAACGTGGAAA+TGG 0.450559 6:-54406018 MsG0680032996.01.T01:CDS
GATTGAACGATCAATTACTA+TGG 0.453291 6:-54405976 MsG0680032996.01.T01:CDS
TGCATGTTCACTTCGGATGA+TGG 0.473045 6:+54405921 None:intergenic
GTCATCGACTAACATGGAAA+TGG 0.499024 6:-54405946 MsG0680032996.01.T01:CDS
GTCAGAGGACGTGAAAGTGC+AGG 0.529090 6:-54406063 MsG0680032996.01.T01:CDS
CTGATCAACTAACATGGAAA+CGG 0.552113 6:-54406090 None:intergenic
ATTAATGAGAACGGTACTAC+GGG 0.555380 6:-54405840 MsG0680032996.01.T01:CDS
AGTGCAGGCTGCACCTAAAG+TGG 0.563132 6:-54406048 MsG0680032996.01.T01:CDS
AATTAATGAGAACGGTACTA+CGG 0.564720 6:-54405841 MsG0680032996.01.T01:CDS
GACCAAGCTGATTGGTGAGG+TGG 0.565992 6:-54405775 MsG0680032996.01.T01:CDS
GTCACCCAAAGTAGACATGC+AGG 0.578396 6:-54405895 MsG0680032996.01.T01:CDS
AGTTGAGTCATCGACTAACA+TGG 0.580553 6:-54405952 MsG0680032996.01.T01:CDS
AGCCACCTCACCAATCAGCT+TGG 0.584705 6:+54405773 None:intergenic
CATGGAAACGGAGCCGTCAG+AGG 0.585593 6:-54406078 MsG0680032996.01.T01:CDS
AAATGGGGATTGTTGATGAA+AGG 0.595868 6:-54405809 MsG0680032996.01.T01:CDS
TGCACTTTCACGTCCTCTGA+CGG 0.602575 6:+54406065 None:intergenic
ATCGCATAACGTGGAAATGG+AGG 0.611040 6:-54406015 MsG0680032996.01.T01:CDS
GAGAATGCAATTAATGAGAA+CGG 0.633127 6:-54405849 MsG0680032996.01.T01:CDS
TGTGCAATCATCGCATAACG+TGG 0.653184 6:-54406024 MsG0680032996.01.T01:CDS
TGACAACTGCATGTTCACTT+CGG 0.664406 6:+54405914 None:intergenic
AAAGACCAAGCTGATTGGTG+AGG 0.672077 6:-54405778 MsG0680032996.01.T01:CDS
CTACGGGTAAACGCAAAATG+GGG 0.679439 6:-54405824 MsG0680032996.01.T01:CDS
AATGGAGGCATCAATTACTG+CGG 0.717083 6:-54406000 MsG0680032996.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATTAATGAGAACGGTACTA+CGG - Chr6:54406006-54406025 MsG0680032996.01.T01:CDS 30.0%
GAGAATGCAATTAATGAGAA+CGG - Chr6:54405998-54406017 MsG0680032996.01.T01:CDS 30.0%
GATTGAACGATCAATTACTA+TGG - Chr6:54405871-54405890 MsG0680032996.01.T01:CDS 30.0%
TTCTCATAAATGTCCAGTTT+AGG + Chr6:54405983-54406002 None:intergenic 30.0%
ATTAATGAGAACGGTACTAC+GGG - Chr6:54406007-54406026 MsG0680032996.01.T01:CDS 35.0%
CATAAAAAGACCAAGCTGAT+TGG - Chr6:54406064-54406083 MsG0680032996.01.T01:CDS 35.0%
! AAATGGGGATTGTTGATGAA+AGG - Chr6:54406038-54406057 MsG0680032996.01.T01:CDS 35.0%
ACTACGGGTAAACGCAAAAT+GGG - Chr6:54406022-54406041 MsG0680032996.01.T01:CDS 40.0%
ATCATCGCATAACGTGGAAA+TGG - Chr6:54405829-54405848 MsG0680032996.01.T01:CDS 40.0%
GATGATTGCACATCCACTTT+AGG + Chr6:54405815-54405834 None:intergenic 40.0%
TACTACGGGTAAACGCAAAA+TGG - Chr6:54406021-54406040 MsG0680032996.01.T01:CDS 40.0%
TGACAACTGCATGTTCACTT+CGG + Chr6:54405936-54405955 None:intergenic 40.0%
! AGTTGAGTCATCGACTAACA+TGG - Chr6:54405895-54405914 MsG0680032996.01.T01:CDS 40.0%
! GTCATCGACTAACATGGAAA+TGG - Chr6:54405901-54405920 MsG0680032996.01.T01:CDS 40.0%
!! AATGGAGGCATCAATTACTG+CGG - Chr6:54405847-54405866 MsG0680032996.01.T01:CDS 40.0%
AAAGACCAAGCTGATTGGTG+AGG - Chr6:54406069-54406088 MsG0680032996.01.T01:CDS 45.0%
ATCGCATAACGTGGAAATGG+AGG - Chr6:54405832-54405851 MsG0680032996.01.T01:CDS 45.0%
CTACGGGTAAACGCAAAATG+GGG - Chr6:54406023-54406042 MsG0680032996.01.T01:CDS 45.0%
TGCATGTTCACTTCGGATGA+TGG + Chr6:54405929-54405948 None:intergenic 45.0%
TGTGCAATCATCGCATAACG+TGG - Chr6:54405823-54405842 MsG0680032996.01.T01:CDS 45.0%
GACGCCTGCATGTCTACTTT+GGG + Chr6:54405959-54405978 None:intergenic 50.0%
GTCACCCAAAGTAGACATGC+AGG - Chr6:54405952-54405971 MsG0680032996.01.T01:CDS 50.0%
TGACGCCTGCATGTCTACTT+TGG + Chr6:54405960-54405979 None:intergenic 50.0%
TGCACTTTCACGTCCTCTGA+CGG + Chr6:54405785-54405804 None:intergenic 50.0%
AGCCACCTCACCAATCAGCT+TGG + Chr6:54406077-54406096 None:intergenic 55.0%
AGTGCAGGCTGCACCTAAAG+TGG - Chr6:54405799-54405818 MsG0680032996.01.T01:CDS 55.0%
CATGCAGGCGTCACCTAAAC+TGG - Chr6:54405967-54405986 MsG0680032996.01.T01:CDS 55.0%
GACCAAGCTGATTGGTGAGG+TGG - Chr6:54406072-54406091 MsG0680032996.01.T01:CDS 55.0%
! GTCAGAGGACGTGAAAGTGC+AGG - Chr6:54405784-54405803 MsG0680032996.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 54405770 54406099 54405770 ID=MsG0680032996.01;Name=MsG0680032996.01
Chr6 mRNA 54405770 54406099 54405770 ID=MsG0680032996.01.T01;Parent=MsG0680032996.01;Name=MsG0680032996.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|109
Chr6 exon 54405770 54406099 54405770 ID=MsG0680032996.01.T01:exon:9251;Parent=MsG0680032996.01.T01
Chr6 CDS 54405770 54406099 54405770 ID=MsG0680032996.01.T01:cds;Parent=MsG0680032996.01.T01
Gene Sequence

>MsG0680032996.01.T01

ATGGAAACGGAGCCGTCAGAGGACGTGAAAGTGCAGGCTGCACCTAAAGTGGATGTGCAATCATCGCATAACGTGGAAATGGAGGCATCAATTACTGCGGAGATTGAACGATCAATTACTATGGAAGTTGAGTCATCGACTAACATGGAAATGGAGCCATCATCCGAAGTGAACATGCAGTTGTCACCCAAAGTAGACATGCAGGCGTCACCTAAACTGGACATTTATGAGAATGCAATTAATGAGAACGGTACTACGGGTAAACGCAAAATGGGGATTGTTGATGAAAGGAAGCATAAAAAGACCAAGCTGATTGGTGAGGTGGCTTAG

Protein sequence

>MsG0680032996.01.T01

METEPSEDVKVQAAPKVDVQSSHNVEMEASITAEIERSITMEVESSTNMEMEPSSEVNMQLSPKVDMQASPKLDIYENAINENGTTGKRKMGIVDERKHKKTKLIGEVA*