AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014809.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAATTGTTCCTGCTTATT+TGG 0.108815 3:+60461644 MsG0380014809.01.T01:CDS
GATTTCATCTCTGAGGCATT+TGG 0.337671 3:+60461615 MsG0380014809.01.T01:CDS
TCCAAATAAGCAGGAACAAT+TGG 0.379299 3:-60461645 None:intergenic
CTACCAGTAGCATCCCCATT+TGG 0.462146 3:-60461567 None:intergenic
TTTCCAAATGGGGATGCTAC+TGG 0.466452 3:+60461564 MsG0380014809.01.T01:CDS
TAAGTAGGATCCAAATAAGC+AGG 0.535765 3:-60461654 None:intergenic
AAATCTGATATATCATAAGT+AGG 0.544264 3:-60461669 None:intergenic
GCTACTGGTAGATTCTCAAA+TGG 0.564673 3:+60461579 MsG0380014809.01.T01:CDS
TGCTACTGATTTCATCTCTG+AGG 0.610128 3:+60461608 MsG0380014809.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTGATATATCATAAGT+AGG - Chr3:60461672-60461691 None:intergenic 20.0%
!! ATGGTAAAGATTTTCCAAAT+GGG + Chr3:60461553-60461572 MsG0380014809.01.T01:CDS 25.0%
! GATATATCAGATTTTGCTAC+TGG + Chr3:60461678-60461697 MsG0380014809.01.T01:CDS 30.0%
! TGGTAAAGATTTTCCAAATG+GGG + Chr3:60461554-60461573 MsG0380014809.01.T01:CDS 30.0%
TAAGTAGGATCCAAATAAGC+AGG - Chr3:60461657-60461676 None:intergenic 35.0%
TCCAAATAAGCAGGAACAAT+TGG - Chr3:60461648-60461667 None:intergenic 35.0%
GCCAATTGTTCCTGCTTATT+TGG + Chr3:60461644-60461663 MsG0380014809.01.T01:CDS 40.0%
GCTACTGGTAGATTCTCAAA+TGG + Chr3:60461579-60461598 MsG0380014809.01.T01:CDS 40.0%
!! GATTTCATCTCTGAGGCATT+TGG + Chr3:60461615-60461634 MsG0380014809.01.T01:CDS 40.0%
!! TGCTACTGATTTCATCTCTG+AGG + Chr3:60461608-60461627 MsG0380014809.01.T01:CDS 40.0%
TTTCCAAATGGGGATGCTAC+TGG + Chr3:60461564-60461583 MsG0380014809.01.T01:CDS 45.0%
! TGTTTTGCTTCTGCTGGTAC+TGG + Chr3:60461705-60461724 MsG0380014809.01.T01:CDS 45.0%
!!! GGTGTTTGTTTTGCTTCTGC+TGG + Chr3:60461699-60461718 MsG0380014809.01.T01:CDS 45.0%
CTACCAGTAGCATCCCCATT+TGG - Chr3:60461570-60461589 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 60461553 60461759 60461553 ID=MsG0380014809.01;Name=MsG0380014809.01
Chr3 mRNA 60461553 60461759 60461553 ID=MsG0380014809.01.T01;Parent=MsG0380014809.01;Name=MsG0380014809.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|68
Chr3 exon 60461553 60461759 60461553 ID=MsG0380014809.01.T01:exon:12251;Parent=MsG0380014809.01.T01
Chr3 CDS 60461553 60461759 60461553 ID=MsG0380014809.01.T01:cds;Parent=MsG0380014809.01.T01
Gene Sequence

>MsG0380014809.01.T01

ATGGTAAAGATTTTCCAAATGGGGATGCTACTGGTAGATTCTCAAATGGTAGAATTGCTACTGATTTCATCTCTGAGGCATTTGGAATTAAGCCAATTGTTCCTGCTTATTTGGATCCTACTTATGATATATCAGATTTTGCTACTGGTGTTTGTTTTGCTTCTGCTGGTACTGGATTTGATAATGCTACTGCAAATGTTGCTGTAA

Protein sequence

>MsG0380014809.01.T01

MVKIFQMGMLLVDSQMVELLLISSLRHLELSQLFLLIWILLMIYQILLLVFVLLLLVLDLIMLLQMLL*