AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053780 MtrunA17_Chr1g0150981 94.595 111 6 0 1 111 1 111 1.44e-63 188
Msa0053780 MtrunA17_Chr1g0151011 93.694 111 7 0 1 111 1 111 7.56e-62 184
Msa0053780 MtrunA17_Chr3g0136311 61.607 112 42 1 1 111 1 112 1.60e-35 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATTAGTGTTACTTTA+TGG 0.143171 1_2:+8273380 Msa0053780:CDS
TCACAAGGTTTGATCGTAAT+TGG 0.180827 1_2:+8273467 Msa0053780:CDS
CATAGAGTTGGTGGTTCTTC+TGG 0.232310 1_2:+8273493 Msa0053780:CDS
TTTACCCTCCTATTTGCTTC+AGG 0.281136 1_2:-8273247 None:intergenic
AACCATAAAGTAACACTAAT+TGG 0.291426 1_2:-8273382 None:intergenic
TGCTAGAACTACTAAGCTTA+TGG 0.394299 1_2:-8273299 None:intergenic
GAATGGAACCTGAAGCAAAT+AGG 0.404269 1_2:+8273239 None:intergenic
AATTGGATTCATAGAGTTGG+TGG 0.508625 1_2:+8273484 Msa0053780:CDS
GATCTGTTATCTATGTATAG+AGG 0.518663 1_2:-8273331 None:intergenic
TAACAGATCAGAGAGTGAGT+TGG 0.526376 1_2:+8273345 Msa0053780:CDS
CGTAATTGGATTCATAGAGT+TGG 0.545772 1_2:+8273481 Msa0053780:CDS
AGAGTTGGTGGTTCTTCTGG+TGG 0.546860 1_2:+8273496 Msa0053780:CDS
TGGAACCTGAAGCAAATAGG+AGG 0.590316 1_2:+8273242 Msa0053780:CDS
GGAACCTGAAGCAAATAGGA+GGG 0.643854 1_2:+8273243 Msa0053780:CDS
AAGCTTAGTAGTTCTAGCAG+TGG 0.645576 1_2:+8273303 Msa0053780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACCAATTAGTGTTACTTTA+TGG + chr1_2:8273380-8273399 Msa0053780:CDS 25.0%
! AACCATAAAGTAACACTAAT+TGG - chr1_2:8273385-8273404 None:intergenic 25.0%
GATCTGTTATCTATGTATAG+AGG - chr1_2:8273334-8273353 None:intergenic 30.0%
!!! CATAGCTTTATCAGCTTTTT+TGG + chr1_2:8273435-8273454 Msa0053780:CDS 30.0%
TGCTAGAACTACTAAGCTTA+TGG - chr1_2:8273302-8273321 None:intergenic 35.0%
!!! ACCTATGCTGCTTTTTGTAT+TGG + chr1_2:8273405-8273424 Msa0053780:CDS 35.0%
ACCAATACAAAAAGCAGCAT+AGG - chr1_2:8273409-8273428 None:intergenic 35.0%
TCACAAGGTTTGATCGTAAT+TGG + chr1_2:8273467-8273486 Msa0053780:CDS 35.0%
CGTAATTGGATTCATAGAGT+TGG + chr1_2:8273481-8273500 Msa0053780:CDS 35.0%
AATTGGATTCATAGAGTTGG+TGG + chr1_2:8273484-8273503 Msa0053780:CDS 35.0%
TTTACCCTCCTATTTGCTTC+AGG - chr1_2:8273250-8273269 None:intergenic 40.0%
!! AAGCTTAGTAGTTCTAGCAG+TGG + chr1_2:8273303-8273322 Msa0053780:CDS 40.0%
TAACAGATCAGAGAGTGAGT+TGG + chr1_2:8273345-8273364 Msa0053780:CDS 40.0%
! TTTTGGACAAGAGCTTCACA+AGG + chr1_2:8273452-8273471 Msa0053780:CDS 40.0%
TGGAACCTGAAGCAAATAGG+AGG + chr1_2:8273242-8273261 Msa0053780:CDS 45.0%
GGAACCTGAAGCAAATAGGA+GGG + chr1_2:8273243-8273262 Msa0053780:CDS 45.0%
!! CATAGAGTTGGTGGTTCTTC+TGG + chr1_2:8273493-8273512 Msa0053780:CDS 45.0%
!! AGAGTTGGTGGTTCTTCTGG+TGG + chr1_2:8273496-8273515 Msa0053780:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8273241 8273576 8273241 ID=Msa0053780;Name=Msa0053780
chr1_2 mRNA 8273241 8273576 8273241 ID=Msa0053780-mRNA-1;Parent=Msa0053780;Name=Msa0053780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|111
chr1_2 exon 8273241 8273576 8273241 ID=Msa0053780-mRNA-1:exon:2935;Parent=Msa0053780-mRNA-1
chr1_2 CDS 8273241 8273576 8273241 ID=Msa0053780-mRNA-1:cds;Parent=Msa0053780-mRNA-1
Gene Sequence

>Msa0053780

ATGGAACCTGAAGCAAATAGGAGGGTAAACAAATTTTCATCTTATGAGAGAGTAGCAGCCATAAGCTTAGTAGTTCTAGCAGTGGCTTCTCCTCTATACATAGATAACAGATCAGAGAGTGAGTTGGAAGATGATGAACAACCAATTAGTGTTACTTTATGGTTACCTATGCTGCTTTTTGTATTGGTTTTAGTCATAGCTTTATCAGCTTTTTTGGACAAGAGCTTCACAAGGTTTGATCGTAATTGGATTCATAGAGTTGGTGGTTCTTCTGGTGGTATTCTTTTCATTCTTATTCTTCTCTTTCTTGTTTTGAAGTGTAAAGCATCTTTGTAA

Protein sequence

>Msa0053780

MEPEANRRVNKFSSYERVAAISLVVLAVASPLYIDNRSESELEDDEQPISVTLWLPMLLFVLVLVIALSAFLDKSFTRFDRNWIHRVGGSSGGILFILILLFLVLKCKASL*