Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275010 | XP_003611244.1 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 2.66e-25 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275010 | sp|Q94AZ4|CML13_ARATH | 80.357 | 56 | 11 | 0 | 47 | 102 | 85 | 140 | 2.33e-24 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275010 | B7FNA2 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 1.27e-25 | 103 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011660 | Msa0275010 | -0.801292 | 9.647916e-49 | -8.615850e-47 |
| Msa0014710 | Msa0275010 | 0.803004 | 4.286358e-49 | -8.615850e-47 |
| Msa0015390 | Msa0275010 | 0.802770 | 4.792683e-49 | -8.615850e-47 |
| Msa0035150 | Msa0275010 | 0.801144 | 1.033990e-48 | -8.615850e-47 |
| Msa0037880 | Msa0275010 | 0.817215 | 3.700837e-52 | -8.615850e-47 |
| Msa0082440 | Msa0275010 | 0.807749 | 4.342004e-50 | -8.615850e-47 |
| Msa0104800 | Msa0275010 | 0.818177 | 2.245861e-52 | -8.615850e-47 |
| Msa0150840 | Msa0275010 | 0.820388 | 7.044379e-53 | -8.615850e-47 |
| Msa0167980 | Msa0275010 | 0.804523 | 2.073987e-49 | -8.615850e-47 |
| Msa0180500 | Msa0275010 | 0.809502 | 1.833331e-50 | -8.615850e-47 |
| Msa0207350 | Msa0275010 | 0.814261 | 1.685703e-51 | -8.615850e-47 |
| Msa0275010 | Msa0275020 | 0.818968 | 1.485835e-52 | -8.615850e-47 |
| Msa0275010 | Msa0285450 | 0.813431 | 2.568906e-51 | -8.615850e-47 |
| Msa0275010 | Msa0315940 | 0.817993 | 2.471175e-52 | -8.615850e-47 |
| Msa0275010 | Msa0319170 | 0.806016 | 1.009424e-49 | -8.615850e-47 |
| Msa0275010 | Msa0359030 | 0.803915 | 2.775552e-49 | -8.615850e-47 |
| Msa0275010 | Msa0379570 | 0.830680 | 2.562068e-55 | -8.615850e-47 |
| Msa0275010 | Msa0435690 | 0.801799 | 7.591086e-49 | -8.615850e-47 |
| Msa0275010 | Msa0444750 | -0.809204 | 2.124397e-50 | -8.615850e-47 |
| Msa0275010 | Msa0467100 | 0.808279 | 3.348968e-50 | -8.615850e-47 |
| Msa0275010 | Msa0468410 | 0.804813 | 1.803592e-49 | -8.615850e-47 |
| Msa0275010 | Msa0488270 | 0.802109 | 6.556341e-49 | -8.615850e-47 |
| Msa0275010 | Msa0517090 | 0.801420 | 9.082802e-49 | -8.615850e-47 |
| Msa0275010 | Msa0523940 | 0.803918 | 2.771063e-49 | -8.615850e-47 |
| Msa0275010 | Msa0529040 | 0.806828 | 6.806643e-50 | -8.615850e-47 |
| Msa0275010 | Msa0705190 | 0.810468 | 1.135424e-50 | -8.615850e-47 |
| Msa0275010 | Msa0731940 | 0.802456 | 5.563254e-49 | -8.615850e-47 |
| Msa0275010 | Msa0748730 | 0.802825 | 4.667005e-49 | -8.615850e-47 |
| Msa0275010 | Msa0748920 | 0.806785 | 6.948204e-50 | -8.615850e-47 |
| Msa0275010 | Msa0760130 | 0.808381 | 3.185178e-50 | -8.615850e-47 |
| Msa0275010 | Msa0768290 | 0.829836 | 4.119465e-55 | -8.615850e-47 |
| Msa0275010 | Msa0794510 | -0.812621 | 3.865768e-51 | -8.615850e-47 |
| Msa0275010 | Msa0807610 | 0.801759 | 7.736020e-49 | -8.615850e-47 |
| Msa0275010 | Msa0820850 | 0.805752 | 1.147094e-49 | -8.615850e-47 |
| Msa0275010 | Msa0832340 | 0.809467 | 1.865062e-50 | -8.615850e-47 |
| Msa0275010 | Msa0844410 | 0.810767 | 9.788179e-51 | -8.615850e-47 |
| Msa0275010 | Msa0844420 | 0.820320 | 7.300995e-53 | -8.615850e-47 |
| Msa0275010 | Msa0844470 | 0.815362 | 9.611443e-52 | -8.615850e-47 |
| Msa0275010 | Msa0844490 | 0.832827 | 7.571469e-56 | -8.615850e-47 |
| Msa0275010 | Msa0857640 | 0.820601 | 6.291696e-53 | -8.615850e-47 |
| Msa0275010 | Msa0861130 | 0.805759 | 1.143176e-49 | -8.615850e-47 |
| Msa0275010 | Msa0864070 | 0.808858 | 2.519426e-50 | -8.615850e-47 |
| Msa0275010 | Msa0866410 | 0.812471 | 4.170209e-51 | -8.615850e-47 |
| Msa0275010 | Msa0899450 | 0.802236 | 6.174828e-49 | -8.615850e-47 |
| Msa0275010 | Msa0903620 | 0.805197 | 1.499285e-49 | -8.615850e-47 |
| Msa0275010 | Msa0927380 | 0.806277 | 8.894204e-50 | -8.615850e-47 |
| Msa0275010 | Msa0936300 | 0.817249 | 3.636148e-52 | -8.615850e-47 |
| Msa0275010 | Msa0960740 | 0.817021 | 4.093086e-52 | -8.615850e-47 |
| Msa0275010 | Msa0962840 | 0.802465 | 5.538476e-49 | -8.615850e-47 |
| Msa0275010 | Msa0966750 | 0.834396 | 3.071678e-56 | -8.615850e-47 |
| Msa0275010 | Msa0969520 | 0.816684 | 4.870498e-52 | -8.615850e-47 |
| Msa0275010 | Msa0973100 | 0.802224 | 6.209626e-49 | -8.615850e-47 |
| Msa0275010 | Msa1012590 | -0.807187 | 5.713368e-50 | -8.615850e-47 |
| Msa0275010 | Msa1054150 | -0.813083 | 3.062393e-51 | -8.615850e-47 |
| Msa0275010 | Msa1063420 | 0.800556 | 1.363726e-48 | -8.615850e-47 |
| Msa0275010 | Msa1121490 | 0.826714 | 2.331222e-54 | -8.615850e-47 |
| Msa0275010 | Msa1137570 | 0.808723 | 2.692629e-50 | -8.615850e-47 |
| Msa0275010 | Msa1211140 | -0.817366 | 3.422361e-52 | -8.615850e-47 |
| Msa0275010 | Msa1215920 | 0.802917 | 4.468834e-49 | -8.615850e-47 |
| Msa0275010 | Msa1284680 | 0.813604 | 2.353363e-51 | -8.615850e-47 |
| Msa0275010 | Msa1299290 | -0.814610 | 1.411599e-51 | -8.615850e-47 |
| Msa0275010 | Msa1299300 | -0.800892 | 1.164232e-48 | -8.615850e-47 |
| Msa0275010 | Msa1306850 | 0.800207 | 1.606388e-48 | -8.615850e-47 |
| Msa0275010 | Msa1345220 | 0.813345 | 2.682499e-51 | -8.615850e-47 |
| Msa0275010 | Msa1353470 | 0.811713 | 6.101248e-51 | -8.615850e-47 |
| Msa0275010 | Msa1357890 | 0.800049 | 1.729713e-48 | -8.615850e-47 |
| Msa0275010 | Msa1358670 | 0.809506 | 1.829529e-50 | -8.615850e-47 |
| Msa0275010 | Msa1394310 | 0.808903 | 2.463887e-50 | -8.615850e-47 |
| Msa0275010 | Msa1424680 | 0.806063 | 9.867013e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275010 | MtrunA17_Chr5g0398751 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 2.44e-29 | 103 |
| Msa0275010 | MtrunA17_Chr5g0440931 | 42.667 | 75 | 40 | 1 | 27 | 101 | 69 | 140 | 6.66e-16 | 68.9 |
| Msa0275010 | MtrunA17_Chr6g0460211 | 42.667 | 75 | 40 | 1 | 27 | 101 | 69 | 140 | 7.49e-16 | 68.9 |
| Msa0275010 | MtrunA17_Chr7g0254731 | 41.333 | 75 | 41 | 1 | 27 | 101 | 69 | 140 | 2.43e-15 | 67.8 |
| Msa0275010 | MtrunA17_Chr3g0093271 | 42.105 | 76 | 41 | 1 | 27 | 102 | 120 | 192 | 5.89e-15 | 67.8 |
| Msa0275010 | MtrunA17_Chr1g0166281 | 34.146 | 82 | 51 | 1 | 21 | 102 | 63 | 141 | 1.56e-13 | 62.8 |
| Msa0275010 | MtrunA17_Chr1g0188171 | 33.333 | 81 | 51 | 1 | 21 | 101 | 63 | 140 | 1.28e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275010 | AT1G12310.1 | 80.357 | 56 | 11 | 0 | 47 | 102 | 85 | 140 | 2.37e-25 | 93.6 |
| Msa0275010 | AT1G62820.1 | 81.132 | 53 | 10 | 0 | 50 | 102 | 88 | 140 | 2.61e-25 | 93.6 |
| Msa0275010 | AT3G56800.1 | 44.000 | 75 | 39 | 1 | 27 | 101 | 69 | 140 | 3.62e-16 | 70.1 |
| Msa0275010 | AT2G41110.1 | 44.000 | 75 | 39 | 1 | 27 | 101 | 69 | 140 | 3.62e-16 | 70.1 |
| Msa0275010 | AT2G27030.1 | 44.000 | 75 | 39 | 1 | 27 | 101 | 69 | 140 | 3.62e-16 | 70.1 |
| Msa0275010 | AT3G43810.4 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.06e-16 | 68.9 |
| Msa0275010 | AT3G43810.3 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.06e-16 | 68.9 |
| Msa0275010 | AT2G27030.3 | 43.421 | 76 | 40 | 1 | 27 | 102 | 69 | 141 | 6.88e-16 | 70.1 |
| Msa0275010 | AT5G21274.1 | 42.667 | 75 | 40 | 1 | 27 | 101 | 69 | 140 | 9.19e-16 | 68.9 |
| Msa0275010 | AT3G43810.1 | 42.667 | 75 | 40 | 1 | 27 | 101 | 69 | 140 | 1.03e-15 | 68.9 |
| Msa0275010 | AT2G41110.2 | 43.421 | 76 | 40 | 1 | 27 | 102 | 81 | 153 | 1.56e-15 | 68.9 |
| Msa0275010 | AT3G43810.2 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 4.75e-15 | 68.6 |
| Msa0275010 | AT1G66410.4 | 39.474 | 76 | 43 | 1 | 27 | 102 | 33 | 105 | 1.17e-14 | 65.5 |
| Msa0275010 | AT1G66410.3 | 39.474 | 76 | 43 | 1 | 27 | 102 | 33 | 105 | 1.17e-14 | 65.5 |
| Msa0275010 | AT5G37780.4 | 39.474 | 76 | 43 | 1 | 27 | 102 | 56 | 128 | 1.90e-14 | 65.5 |
| Msa0275010 | AT1G66410.1 | 39.474 | 76 | 43 | 1 | 27 | 102 | 69 | 141 | 1.94e-14 | 65.5 |
| Msa0275010 | AT5G37780.1 | 39.474 | 76 | 43 | 1 | 27 | 102 | 69 | 141 | 1.94e-14 | 65.5 |
| Msa0275010 | AT1G66410.2 | 39.474 | 76 | 43 | 1 | 27 | 102 | 79 | 151 | 1.98e-14 | 65.9 |
| Msa0275010 | AT5G37780.2 | 39.474 | 76 | 43 | 1 | 27 | 102 | 84 | 156 | 2.67e-14 | 65.5 |
| Msa0275010 | AT5G37780.3 | 39.474 | 76 | 43 | 1 | 27 | 102 | 95 | 167 | 4.37e-14 | 65.5 |
| Msa0275010 | AT3G22930.1 | 32.222 | 90 | 58 | 1 | 12 | 101 | 77 | 163 | 6.94e-13 | 62.0 |
| Msa0275010 | AT4G14640.2 | 30.769 | 91 | 60 | 1 | 12 | 102 | 37 | 124 | 3.77e-12 | 59.3 |
| Msa0275010 | AT4G14640.1 | 30.769 | 91 | 60 | 1 | 12 | 102 | 55 | 142 | 6.11e-12 | 59.3 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATGGATTTGAGTTGGGCT+TGG | 0.312614 | 2_3:-6911851 | None:intergenic |
| ATTTGAGTTGGGCTTGGGTC+GGG | 0.416027 | 2_3:-6911845 | None:intergenic |
| CGGCGACGATGGATTTGAGT+TGG | 0.421727 | 2_3:-6911857 | None:intergenic |
| GATGGATTTGAGTTGGGCTT+GGG | 0.428812 | 2_3:-6911850 | None:intergenic |
| CGTATTAAAGAAGTTGATGT+TGG | 0.442680 | 2_3:+6912006 | Msa0275010:CDS |
| GAAGTTGATGTTGGGTCTGA+TGG | 0.446161 | 2_3:+6912015 | Msa0275010:CDS |
| GATTTGAGTTGGGCTTGGGT+CGG | 0.446248 | 2_3:-6911846 | None:intergenic |
| GGCGACGATGGATTTGAGTT+GGG | 0.457758 | 2_3:-6911856 | None:intergenic |
| GTTCTCGATAAAGATGCTAC+TGG | 0.465422 | 2_3:+6911907 | Msa0275010:CDS |
| GAAATCGAAAGGAGAACATT+CGG | 0.467430 | 2_3:-6911877 | None:intergenic |
| TACCGCCGAGAGAAAGCAAC+AGG | 0.474309 | 2_3:-6911821 | None:intergenic |
| GGAATCCTGTTGCTTTCTCT+CGG | 0.475828 | 2_3:+6911816 | Msa0275010:CDS |
| AGGAGAACATTCGGCGACGA+TGG | 0.475961 | 2_3:-6911868 | None:intergenic |
| AAGATCGCACCATCGAAGCT+TGG | 0.500822 | 2_3:+6911795 | Msa0275010:CDS |
| ATACGTTCATCAAACTCAGC+AGG | 0.514307 | 2_3:-6911988 | None:intergenic |
| ATGAAGATTCATCGCAAGAA+TGG | 0.515839 | 2_3:+6912049 | Msa0275010:CDS |
| AAACAATTGCATTCATCACT+TGG | 0.521261 | 2_3:-6912075 | None:intergenic |
| TGCGATCTTTCCGTCGTCGT+CGG | 0.547536 | 2_3:-6911781 | None:intergenic |
| GTATTAAAGAAGTTGATGTT+GGG | 0.551236 | 2_3:+6912007 | Msa0275010:CDS |
| CTTGCGATGAATCTTCATAA+CGG | 0.557826 | 2_3:-6912044 | None:intergenic |
| AAGTTGATGTTGGGTCTGAT+GGG | 0.577489 | 2_3:+6912016 | Msa0275010:CDS |
| GAACTTTGAGAGAAATCGAA+AGG | 0.580421 | 2_3:-6911888 | None:intergenic |
| AACAGGATTCCAAGCTTCGA+TGG | 0.609426 | 2_3:-6911804 | None:intergenic |
| ATCCTGTTGCTTTCTCTCGG+CGG | 0.610054 | 2_3:+6911819 | Msa0275010:CDS |
| CTCTTCGACACCGACGACGA+CGG | 0.633183 | 2_3:+6911771 | Msa0275010:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTATTAAAGAAGTTGATGTT+GGG | + | chr2_3:6912007-6912026 | Msa0275010:CDS | 25.0% |
| !! | CGTATTAAAGAAGTTGATGT+TGG | + | chr2_3:6912006-6912025 | Msa0275010:CDS | 30.0% |
| AAACAATTGCATTCATCACT+TGG | - | chr2_3:6912078-6912097 | None:intergenic | 30.0% | |
| GAAATCGAAAGGAGAACATT+CGG | - | chr2_3:6911880-6911899 | None:intergenic | 35.0% | |
| ! | GAACTTTGAGAGAAATCGAA+AGG | - | chr2_3:6911891-6911910 | None:intergenic | 35.0% |
| !!! | CGTCATATTTTGACGAGTAT+TGG | + | chr2_3:6911952-6911971 | Msa0275010:CDS | 35.0% |
| CTTGCGATGAATCTTCATAA+CGG | - | chr2_3:6912047-6912066 | None:intergenic | 35.0% | |
| ATGAAGATTCATCGCAAGAA+TGG | + | chr2_3:6912049-6912068 | Msa0275010:CDS | 35.0% | |
| GTTCTCGATAAAGATGCTAC+TGG | + | chr2_3:6911907-6911926 | Msa0275010:CDS | 40.0% | |
| ATACGTTCATCAAACTCAGC+AGG | - | chr2_3:6911991-6912010 | None:intergenic | 40.0% | |
| !! | AAGTTGATGTTGGGTCTGAT+GGG | + | chr2_3:6912016-6912035 | Msa0275010:CDS | 40.0% |
| AACAGGATTCCAAGCTTCGA+TGG | - | chr2_3:6911807-6911826 | None:intergenic | 45.0% | |
| !! | GGAATCCTGTTGCTTTCTCT+CGG | + | chr2_3:6911816-6911835 | Msa0275010:CDS | 45.0% |
| !! | GATGGATTTGAGTTGGGCTT+GGG | - | chr2_3:6911853-6911872 | None:intergenic | 45.0% |
| !! | GAAGTTGATGTTGGGTCTGA+TGG | + | chr2_3:6912015-6912034 | Msa0275010:CDS | 45.0% |
| AAGATCGCACCATCGAAGCT+TGG | + | chr2_3:6911795-6911814 | Msa0275010:CDS | 50.0% | |
| !! | ATCCTGTTGCTTTCTCTCGG+CGG | + | chr2_3:6911819-6911838 | Msa0275010:CDS | 50.0% |
| !! | ATTTGAGTTGGGCTTGGGTC+GGG | - | chr2_3:6911848-6911867 | None:intergenic | 50.0% |
| !! | GATTTGAGTTGGGCTTGGGT+CGG | - | chr2_3:6911849-6911868 | None:intergenic | 50.0% |
| !! | CGATGGATTTGAGTTGGGCT+TGG | - | chr2_3:6911854-6911873 | None:intergenic | 50.0% |
| ! | GGCGACGATGGATTTGAGTT+GGG | - | chr2_3:6911859-6911878 | None:intergenic | 50.0% |
| TGCGATCTTTCCGTCGTCGT+CGG | - | chr2_3:6911784-6911803 | None:intergenic | 55.0% | |
| TACCGCCGAGAGAAAGCAAC+AGG | - | chr2_3:6911824-6911843 | None:intergenic | 55.0% | |
| ! | CGGCGACGATGGATTTGAGT+TGG | - | chr2_3:6911860-6911879 | None:intergenic | 55.0% |
| AGGAGAACATTCGGCGACGA+TGG | - | chr2_3:6911871-6911890 | None:intergenic | 55.0% | |
| CTCTTCGACACCGACGACGA+CGG | + | chr2_3:6911771-6911790 | Msa0275010:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 6911751 | 6912116 | 6911751 | ID=Msa0275010;Name=Msa0275010 |
| chr2_3 | mRNA | 6911751 | 6912116 | 6911751 | ID=Msa0275010-mRNA-1;Parent=Msa0275010;Name=Msa0275010-mRNA-1;_AED=0.10;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|121 |
| chr2_3 | exon | 6911751 | 6912116 | 6911751 | ID=Msa0275010-mRNA-1:exon:3281;Parent=Msa0275010-mRNA-1 |
| chr2_3 | CDS | 6911751 | 6912116 | 6911751 | ID=Msa0275010-mRNA-1:cds;Parent=Msa0275010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
