AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0747630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0747630 MtrunA17_Chr3g0132801 95.516 223 0 2 1 213 1 223 1.44e-151 419
Msa0747630 MtrunA17_Chr8g0367261 38.725 204 102 7 18 206 13 208 3.15e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0747630 AT5G65520.1 40.625 192 99 5 18 197 12 200 3.56e-36 126

Find 55 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGTCATTTCGGTGTTTCT+TGG 0.193148 5_2:+418214 Msa0747630:three_prime_UTR
AGATACGAAGCGGCGTCTTT+TGG 0.244593 5_2:-415641 Msa0747620:three_prime_UTR
CCCTAGTGTTGGTTTCTACT+TGG 0.278297 5_2:+418343 Msa0747630:three_prime_UTR
TAGTGATGTATAACAAATTA+TGG 0.290086 5_2:-418379 Msa0747620:three_prime_UTR
CACCAAGCTTTCGCGTTCTT+CGG 0.307624 5_2:-415875 Msa0747620:three_prime_UTR
GGAAGCAAAGAAAGCTTATA+AGG 0.314616 5_2:+415937 Msa0747630:CDS
GTGTTATGAAGGGAAGAAAA+TGG 0.346267 5_2:+415910 Msa0747630:CDS
CGGTGTTTCTTGGCTTTGGC+AGG 0.347122 5_2:+418224 Msa0747630:three_prime_UTR
TTCGTTTGCGAGGTTGTTGA+TGG 0.363091 5_2:-415582 Msa0747620:three_prime_UTR
ATTTCGGTGTTTCTTGGCTT+TGG 0.368482 5_2:+418220 Msa0747630:three_prime_UTR
GCTATGGCACCATGTCATTT+CGG 0.393391 5_2:+418204 Msa0747630:three_prime_UTR
TGCCTGTAGAAGCACTTAAC+AGG 0.416791 5_2:+415990 Msa0747630:CDS
GAGGTTGTTGATGGCGGATC+TGG 0.441588 5_2:-415573 Msa0747620:three_prime_UTR
GTCCGAAGAACGCGAAAGCT+TGG 0.448178 5_2:+415873 Msa0747630:CDS
GCGAAAGCTTGGTGTGCGTT+GGG 0.450050 5_2:+415884 Msa0747630:CDS
CGCGAAAGCTTGGTGTGCGT+TGG 0.456259 5_2:+415883 Msa0747630:CDS
TTATAAGGAAGCTCATGAAT+TGG 0.465517 5_2:+415952 Msa0747630:CDS
GAAAGGTGTTTCATTAGCTA+TGG 0.469154 5_2:+418188 Msa0747630:three_prime_UTR
GCTATGATTCTCGATCTTCA+AGG 0.478244 5_2:+415671 Msa0747630:CDS
AATGTGGCGGCGCTTTGTTA+AGG 0.496636 5_2:+418259 Msa0747630:three_prime_UTR
ACGAGTTGACTCGGCGTTAG+CGG 0.514403 5_2:+415826 Msa0747630:CDS
CGCCTAAGGCTTCGTTTGCG+AGG 0.524088 5_2:-415592 Msa0747620:three_prime_UTR
CAGCAATGTAACAAAAGATA+CGG 0.526619 5_2:+418127 Msa0747630:intron
AAATTTGTGTACCCTAGTGT+TGG 0.542240 5_2:+418332 Msa0747630:three_prime_UTR
TATTGAGGAAGAATGTATGT+TGG 0.544110 5_2:+418166 Msa0747630:three_prime_UTR
TTAATCTTCCTCAGCATGCA+CGG 0.553368 5_2:+415431 Msa0747630:CDS
GCGTTGTCTCCTCTCGCCGC+CGG 0.558332 5_2:+415728 Msa0747630:CDS
GCGTTGGGAGAGTGTTATGA+AGG 0.558916 5_2:+415899 Msa0747630:CDS
TGAACGCGGACGAGTTGACT+CGG 0.566518 5_2:+415817 Msa0747630:CDS
CAAGCGATGTACCGCGACAT+CGG 0.569247 5_2:+415377 Msa0747630:CDS
TGAAGGGAAGAAAATGGATG+AGG 0.575977 5_2:+415916 Msa0747630:CDS
AACCTGTTAAGTGCTTCTAC+AGG 0.580757 5_2:-415992 Msa0747620:three_prime_UTR
AGGAAGAATGTATGTTGGAA+AGG 0.594020 5_2:+418171 Msa0747630:three_prime_UTR
TTTGGAATCGGAGTTTAGCG+AGG 0.596238 5_2:-415469 Msa0747620:three_prime_UTR
GTTTGCGAGGTTGTTGATGG+CGG 0.604157 5_2:-415579 Msa0747620:three_prime_UTR
TCAAGCGAACCGGCGGCGAG+AGG 0.609088 5_2:-415737 Msa0747620:three_prime_UTR
GCAGTCTTGAACTTCTATTG+AGG 0.611422 5_2:+418151 Msa0747630:exon
CAAGAAACACCGAAATGACA+TGG 0.618215 5_2:-418213 Msa0747620:three_prime_UTR
ACCAAGTAGAAACCAACACT+AGG 0.619181 5_2:-418344 Msa0747620:three_prime_UTR
CCAAGTAGAAACCAACACTA+GGG 0.636091 5_2:-418343 Msa0747620:three_prime_UTR
AGTCATCATGATCCTCGCAA+TGG 0.640456 5_2:+415406 Msa0747630:CDS
TCGCTTGAGCAAGGTGAACG+TGG 0.649304 5_2:+415752 Msa0747630:intron
GATGTAAGAAGCAAATGTGG+CGG 0.650810 5_2:+418246 Msa0747630:three_prime_UTR
CGTTGGGAGAGTGTTATGAA+GGG 0.659064 5_2:+415900 Msa0747630:CDS
GTGGAATTCCGTGCATGCTG+AGG 0.659142 5_2:-415439 Msa0747620:three_prime_UTR
AACCTCGCAAACGAAGCCTT+AGG 0.661102 5_2:+415590 Msa0747630:CDS
CAGGATGTAAGAAGCAAATG+TGG 0.661856 5_2:+418243 Msa0747630:three_prime_UTR
GTTAGCGGATTTGACTGAGT+CGG 0.668658 5_2:+415841 Msa0747630:CDS
AGCTTTGAGAAGATACGAAG+CGG 0.671113 5_2:-415651 Msa0747620:three_prime_UTR
AAACTCGCAACGAGTGAACG+CGG 0.681953 5_2:+415803 Msa0747630:CDS
GGAAGATTAAAGCCATTGCG+AGG 0.704504 5_2:-415418 Msa0747620:three_prime_UTR
TGACTTGTAAACCGATGTCG+CGG 0.717200 5_2:-415388 Msa0747620:three_prime_UTR
GGCGTCGAGTGATTCAAGAG+CGG 0.721407 5_2:-415705 Msa0747620:three_prime_UTR
TCTACAGGCACAGAATACTG+TGG 0.738815 5_2:-415977 Msa0747620:three_prime_UTR
GGCGGCGAGAGGAGACAACG+CGG 0.761382 5_2:-415726 Msa0747620:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTATATAAATGTAATAAA+AGG + chr5_2:416607-416626 Msa0747630:intron 10.0%
!! GTTATATAAATGTAATAAAA+GGG + chr5_2:416608-416627 Msa0747630:intron 10.0%
!!! TATTTTTTTTTAGAGATTAA+AGG + chr5_2:417772-417791 Msa0747630:intron 10.0%
!!! TTTTTTTTTTTCATGAACTT+TGG + chr5_2:417795-417814 Msa0747630:intron 15.0%
!!! TTTTTTTTTTCATGAACTTT+GGG + chr5_2:417796-417815 Msa0747630:intron 15.0%
!! ATGCTAAATTAAAAGACTTA+TGG + chr5_2:416091-416110 Msa0747630:intron 20.0%
!! TGCTAAATTAAAAGACTTAT+GGG + chr5_2:416092-416111 Msa0747630:intron 20.0%
!!! CGTGTTAATTTTATAAATGT+GGG - chr5_2:416647-416666 Msa0747620:intron 20.0%
!!! ACGTGTTAATTTTATAAATG+TGG - chr5_2:416648-416667 Msa0747620:intron 20.0%
!! GCTATTTACAGATTTATTAT+AGG + chr5_2:416789-416808 Msa0747630:intron 20.0%
!! AAAATAGACAAATTCAAACT+TGG - chr5_2:417023-417042 Msa0747620:intron 20.0%
!!! TTTTTGAAAGAGAATATTAC+TGG - chr5_2:417145-417164 Msa0747620:intron 20.0%
!!! TTATAATTTGCCAAATTTCA+AGG + chr5_2:417225-417244 Msa0747630:intron 20.0%
!!! TTTTTTTCATCAACAACAAT+AGG + chr5_2:418053-418072 Msa0747630:intron 20.0%
!! TAGTGATGTATAACAAATTA+TGG - chr5_2:418382-418401 Msa0747620:three_prime_UTR 20.0%
! GAAATATTTATTCTTCCTTC+TGG + chr5_2:416220-416239 Msa0747630:intron 25.0%
!! TGAAGTTAAGTTGTGTTAAA+AGG + chr5_2:416352-416371 Msa0747630:intron 25.0%
!!! AGGACATTTTTTGCTAAAAT+TGG + chr5_2:416375-416394 Msa0747630:intron 25.0%
!! ATTTTTAATCTCCTTTCGTT+TGG + chr5_2:416579-416598 Msa0747630:intron 25.0%
!! ATAACATTTTACCAAACGAA+AGG - chr5_2:416593-416612 Msa0747620:intron 25.0%
!!! TGAACTCTATTTTCTTGTTT+TGG - chr5_2:416762-416781 Msa0747620:intron 25.0%
! TGATCATCATCTAAAAATGT+TGG + chr5_2:416960-416979 Msa0747630:intron 25.0%
! GGATGTCAATATTGAAAATT+TGG + chr5_2:416995-417014 Msa0747630:intron 25.0%
!! AAACAAAAGTGTGAAAATCT+AGG - chr5_2:417068-417087 Msa0747620:intron 25.0%
!!! TTTAGCTTTTGATTCACAAA+TGG + chr5_2:417621-417640 Msa0747630:intron 25.0%
! ATACAATGCTTAAACTGTTT+AGG - chr5_2:417722-417741 Msa0747620:intron 25.0%
!!! GTGAAGACTTGTTTTTATTA+TGG - chr5_2:417836-417855 Msa0747620:intron 25.0%
TTATAAGGAAGCTCATGAAT+TGG + chr5_2:415952-415971 Msa0747630:CDS 30.0%
TAGGCGTTAATAAATGATTC+TGG + chr5_2:416122-416141 Msa0747630:intron 30.0%
TATCATGAAGAGCAAAGTTT+CGG + chr5_2:416178-416197 Msa0747630:intron 30.0%
! AGTTAAGTTGTGTTAAAAGG+AGG + chr5_2:416355-416374 Msa0747630:intron 30.0%
TTGAACGTAATCACATGTAT+CGG + chr5_2:416469-416488 Msa0747630:intron 30.0%
AAAAGCGATTAAAGCAAACA+TGG - chr5_2:416538-416557 Msa0747620:intron 30.0%
AGGACTACGAATTATTCAAA+AGG + chr5_2:416847-416866 Msa0747630:intron 30.0%
AATATTGACATCCAAGTTAC+TGG - chr5_2:416988-417007 Msa0747620:intron 30.0%
! AATTGGCCATTGATAATAGA+AGG - chr5_2:417197-417216 Msa0747620:intron 30.0%
AAGAGATACACCTTGAAATT+TGG - chr5_2:417238-417257 Msa0747620:intron 30.0%
TCATGAAATTGCAAGTGAAA+GGG - chr5_2:417302-417321 Msa0747620:intron 30.0%
!! CTTCACTTGTTTTCTCTTTT+GGG + chr5_2:417413-417432 Msa0747630:intron 30.0%
AAGAGGCTAAAAGTAGAAAT+TGG + chr5_2:417469-417488 Msa0747630:intron 30.0%
ATGCTTAAACTGTTTAGGTA+GGG - chr5_2:417717-417736 Msa0747620:intron 30.0%
AATGCTTAAACTGTTTAGGT+AGG - chr5_2:417718-417737 Msa0747620:intron 30.0%
AACTATTGACCAACAATTCA+TGG + chr5_2:417971-417990 Msa0747630:intron 30.0%
AAGTTAGTATCAGAGACAAT+CGG - chr5_2:418089-418108 Msa0747620:intron 30.0%
CAGCAATGTAACAAAAGATA+CGG + chr5_2:418127-418146 Msa0747630:intron 30.0%
TATTGAGGAAGAATGTATGT+TGG + chr5_2:418166-418185 Msa0747630:three_prime_UTR 30.0%
! AGTTTAGCGAGGAATTTTTG+TGG - chr5_2:415461-415480 Msa0747620:three_prime_UTR 35.0%
!!! TTGCTTTTCGGTTTTGGAAT+CGG - chr5_2:415484-415503 Msa0747620:three_prime_UTR 35.0%
!! CTTTGATTGCTTTTCGGTTT+TGG - chr5_2:415490-415509 Msa0747620:three_prime_UTR 35.0%
!! GCTTTGCTTTGATTGCTTTT+CGG - chr5_2:415496-415515 Msa0747620:three_prime_UTR 35.0%
GTGTTATGAAGGGAAGAAAA+TGG + chr5_2:415910-415929 Msa0747630:CDS 35.0%
GGAAGCAAAGAAAGCTTATA+AGG + chr5_2:415937-415956 Msa0747630:CDS 35.0%
CGTTTCTTTCTTAATCCAGA+AGG - chr5_2:416238-416257 Msa0747620:intron 35.0%
!!! GTTGGTTTTGAAACAACGAA+CGG - chr5_2:416301-416320 Msa0747620:intron 35.0%
!!! CCTCTCGTTTTATTTTCAGT+TGG - chr5_2:416319-416338 Msa0747620:intron 35.0%
CCAACTGAAAATAAAACGAG+AGG + chr5_2:416316-416335 Msa0747630:intron 35.0%
AATAAAACGAGAGGAACATC+AGG + chr5_2:416325-416344 Msa0747630:intron 35.0%
!! GTTTGAAGTGATTTTGAGAG+TGG + chr5_2:416704-416723 Msa0747630:intron 35.0%
! ATATTTTATCGTGAGAGGAG+AGG - chr5_2:416738-416757 Msa0747620:intron 35.0%
! TTGGCATATTTTATCGTGAG+AGG - chr5_2:416743-416762 Msa0747620:intron 35.0%
TGTGGCATGGAAAATTGAAA+AGG - chr5_2:416818-416837 Msa0747620:intron 35.0%
ATTCAAAAGGTGAGTTAGTG+TGG + chr5_2:416860-416879 Msa0747630:intron 35.0%
AAAACCAAAACCAGATTGTG+GGG - chr5_2:416896-416915 Msa0747620:intron 35.0%
CAAAACCAAAACCAGATTGT+GGG - chr5_2:416897-416916 Msa0747620:intron 35.0%
AAATGTTGGAACCAGTAACT+TGG + chr5_2:416974-416993 Msa0747630:intron 35.0%
GCTTCTCCTTCTATTATCAA+TGG + chr5_2:417188-417207 Msa0747630:intron 35.0%
GTCATGAAATTGCAAGTGAA+AGG - chr5_2:417303-417322 Msa0747620:intron 35.0%
CAAGTGAAGGAACAAAGTAA+AGG - chr5_2:417402-417421 Msa0747620:intron 35.0%
CCAAAAGAGAAAACAAGTGA+AGG - chr5_2:417415-417434 Msa0747620:intron 35.0%
!! CCTTCACTTGTTTTCTCTTT+TGG + chr5_2:417412-417431 Msa0747630:intron 35.0%
AGAAATGTTAACCAATGTCC+GGG - chr5_2:417593-417612 Msa0747620:intron 35.0%
GAGAAATGTTAACCAATGTC+CGG - chr5_2:417594-417613 Msa0747620:intron 35.0%
TAAAAACAAGTCTTCACTCG+TGG + chr5_2:417839-417858 Msa0747630:intron 35.0%
AAAAACAAGTCTTCACTCGT+GGG + chr5_2:417840-417859 Msa0747630:intron 35.0%
TATACAAGCGAAAAGACATG+AGG - chr5_2:417915-417934 Msa0747620:intron 35.0%
AGGAAGAATGTATGTTGGAA+AGG + chr5_2:418171-418190 Msa0747630:three_prime_UTR 35.0%
!! GAAAGGTGTTTCATTAGCTA+TGG + chr5_2:418188-418207 Msa0747630:three_prime_UTR 35.0%
AAATTTGTGTACCCTAGTGT+TGG + chr5_2:418332-418351 Msa0747630:three_prime_UTR 35.0%
TTAATCTTCCTCAGCATGCA+CGG + chr5_2:415431-415450 Msa0747630:CDS 40.0%
!!! ATCGAGTTCGATCGCTTTTT+CGG - chr5_2:415621-415640 Msa0747620:three_prime_UTR 40.0%
!! AGCTTTGAGAAGATACGAAG+CGG - chr5_2:415654-415673 Msa0747620:three_prime_UTR 40.0%
GCTATGATTCTCGATCTTCA+AGG + chr5_2:415671-415690 Msa0747630:CDS 40.0%
TGAAGGGAAGAAAATGGATG+AGG + chr5_2:415916-415935 Msa0747630:CDS 40.0%
AACCTGTTAAGTGCTTCTAC+AGG - chr5_2:415995-416014 Msa0747620:intron 40.0%
!! TGTAGAAGCACTTAACAGGT+TGG + chr5_2:415994-416013 Msa0747630:intron 40.0%
!! GTAGAAGCACTTAACAGGTT+GGG + chr5_2:415995-416014 Msa0747630:intron 40.0%
AAGACTTATGGGTCAAGAGT+AGG + chr5_2:416103-416122 Msa0747630:intron 40.0%
! TAATCACATGTATCGGTGTC+AGG + chr5_2:416476-416495 Msa0747630:intron 40.0%
TCCATGCCACAATCGTAATT+AGG + chr5_2:416827-416846 Msa0747630:intron 40.0%
TCCTAATTACGATTGTGGCA+TGG - chr5_2:416831-416850 Msa0747620:intron 40.0%
CGTAGTCCTAATTACGATTG+TGG - chr5_2:416836-416855 Msa0747620:intron 40.0%
CCAAAACCAAAACCAGATTG+TGG - chr5_2:416898-416917 Msa0747620:intron 40.0%
!!! CCACAATCTGGTTTTGGTTT+TGG + chr5_2:416895-416914 Msa0747630:intron 40.0%
GTGAAAATCTAGGCATGTAG+TGG - chr5_2:417058-417077 Msa0747620:intron 40.0%
AAAAACACGAGAAGCCACAT+GGG + chr5_2:417160-417179 Msa0747630:intron 40.0%
TCAAGACAGCAAAAGTTGCA+CGG - chr5_2:417261-417280 Msa0747620:intron 40.0%
TCACTTGCAATTTCATGACG+TGG + chr5_2:417304-417323 Msa0747630:intron 40.0%
ATGACGTGGTTGTGAATTCA+TGG + chr5_2:417318-417337 Msa0747630:intron 40.0%
GTTGTGAATTCATGGAAGCA+TGG + chr5_2:417326-417345 Msa0747630:intron 40.0%
!! CTCTTTTGGGTCATGTTAAC+CGG + chr5_2:417426-417445 Msa0747630:intron 40.0%
AGCCTCTTTAAACAATGTCC+CGG - chr5_2:417457-417476 Msa0747620:intron 40.0%
GAAATGTTAACCAATGTCCG+GGG - chr5_2:417592-417611 Msa0747620:intron 40.0%
ACTCGTGGGTTTGTATTAGT+GGG + chr5_2:417854-417873 Msa0747630:intron 40.0%
ACCAACAATTCATGGCATCA+CGG + chr5_2:417979-417998 Msa0747630:intron 40.0%
TTAGTATCAGAGACAATCGG+TGG - chr5_2:418086-418105 Msa0747620:intron 40.0%
GCAGTCTTGAACTTCTATTG+AGG + chr5_2:418151-418170 Msa0747630:exon 40.0%
CAAGAAACACCGAAATGACA+TGG - chr5_2:418216-418235 Msa0747620:three_prime_UTR 40.0%
!! CATGTCATTTCGGTGTTTCT+TGG + chr5_2:418214-418233 Msa0747630:three_prime_UTR 40.0%
!! ATTTCGGTGTTTCTTGGCTT+TGG + chr5_2:418220-418239 Msa0747630:three_prime_UTR 40.0%
CAGGATGTAAGAAGCAAATG+TGG + chr5_2:418243-418262 Msa0747630:three_prime_UTR 40.0%
GATGTAAGAAGCAAATGTGG+CGG + chr5_2:418246-418265 Msa0747630:three_prime_UTR 40.0%
CCAAGTAGAAACCAACACTA+GGG - chr5_2:418346-418365 Msa0747620:three_prime_UTR 40.0%
ACCAAGTAGAAACCAACACT+AGG - chr5_2:418347-418366 Msa0747620:three_prime_UTR 40.0%
! TGACTTGTAAACCGATGTCG+CGG - chr5_2:415391-415410 Msa0747620:three_prime_UTR 45.0%
AGTCATCATGATCCTCGCAA+TGG + chr5_2:415406-415425 Msa0747630:CDS 45.0%
GGAAGATTAAAGCCATTGCG+AGG - chr5_2:415421-415440 Msa0747620:three_prime_UTR 45.0%
! TTTGGAATCGGAGTTTAGCG+AGG - chr5_2:415472-415491 Msa0747620:three_prime_UTR 45.0%
!! GGCGGATCTGGATTTGTTTT+TGG - chr5_2:415564-415583 Msa0747620:three_prime_UTR 45.0%
TTCGTTTGCGAGGTTGTTGA+TGG - chr5_2:415585-415604 Msa0747620:three_prime_UTR 45.0%
!! GTTAGCGGATTTGACTGAGT+CGG + chr5_2:415841-415860 Msa0747630:CDS 45.0%
!! CGTTGGGAGAGTGTTATGAA+GGG + chr5_2:415900-415919 Msa0747630:CDS 45.0%
TCTACAGGCACAGAATACTG+TGG - chr5_2:415980-415999 Msa0747620:three_prime_UTR 45.0%
!! TGCCTGTAGAAGCACTTAAC+AGG + chr5_2:415990-416009 Msa0747630:CDS 45.0%
!! TGAACCCCACAATCTGGTTT+TGG + chr5_2:416889-416908 Msa0747630:intron 45.0%
GGTGCTAAATTAGACAGACG+TGG - chr5_2:417106-417125 Msa0747620:intron 45.0%
ACTGGTAAGTAGCAACGACT+CGG - chr5_2:417127-417146 Msa0747620:intron 45.0%
CAAAAACACGAGAAGCCACA+TGG + chr5_2:417159-417178 Msa0747630:intron 45.0%
CTCTTTAAACAATGTCCCGG+AGG - chr5_2:417454-417473 Msa0747620:intron 45.0%
CTCCGGGACATTGTTTAAAG+AGG + chr5_2:417452-417471 Msa0747630:intron 45.0%
CACTCGTGGGTTTGTATTAG+TGG + chr5_2:417853-417872 Msa0747630:intron 45.0%
AGCGAAAAGACATGAGGTTG+AGG - chr5_2:417909-417928 Msa0747620:intron 45.0%
ACCGTGATGCCATGAATTGT+TGG - chr5_2:417983-418002 Msa0747620:intron 45.0%
CAATTCATGGCATCACGGTA+TGG + chr5_2:417984-418003 Msa0747630:intron 45.0%
! GCTATGGCACCATGTCATTT+CGG + chr5_2:418204-418223 Msa0747630:three_prime_UTR 45.0%
!! CCCTAGTGTTGGTTTCTACT+TGG + chr5_2:418343-418362 Msa0747630:three_prime_UTR 45.0%
!! AAATTATAAAATACAAAAAT+TGG - chr5_2:417214-417233 Msa0747620:intron 5.0%
! GTTTGCGAGGTTGTTGATGG+CGG - chr5_2:415582-415601 Msa0747620:three_prime_UTR 50.0%
AACCTCGCAAACGAAGCCTT+AGG + chr5_2:415590-415609 Msa0747630:CDS 50.0%
! AGATACGAAGCGGCGTCTTT+TGG - chr5_2:415644-415663 Msa0747620:three_prime_UTR 50.0%
TTCACCTTGCTCAAGCGAAC+CGG - chr5_2:415750-415769 Msa0747620:three_prime_UTR 50.0%
AAACTCGCAACGAGTGAACG+CGG + chr5_2:415803-415822 Msa0747630:CDS 50.0%
CACCAAGCTTTCGCGTTCTT+CGG - chr5_2:415878-415897 Msa0747620:three_prime_UTR 50.0%
!! GCGTTGGGAGAGTGTTATGA+AGG + chr5_2:415899-415918 Msa0747630:CDS 50.0%
AGGTCACTGCTGTTAGACAG+CGG + chr5_2:416496-416515 Msa0747630:intron 50.0%
ACTACGTGAACCCCACAATC+TGG + chr5_2:416883-416902 Msa0747630:intron 50.0%
AGGAGAAGCAACATCCCATG+TGG - chr5_2:417177-417196 Msa0747620:intron 50.0%
TGTTTCCTTAACCAGTGCCC+CGG + chr5_2:417572-417591 Msa0747630:intron 50.0%
! AATGTGGCGGCGCTTTGTTA+AGG + chr5_2:418259-418278 Msa0747630:three_prime_UTR 50.0%
CAAGCGATGTACCGCGACAT+CGG + chr5_2:415377-415396 Msa0747630:CDS 55.0%
GTGGAATTCCGTGCATGCTG+AGG - chr5_2:415442-415461 Msa0747620:three_prime_UTR 55.0%
! GCAAAGCGTCACTTCGTACG+TGG + chr5_2:415509-415528 Msa0747630:intron 55.0%
!! GAGGTTGTTGATGGCGGATC+TGG - chr5_2:415576-415595 Msa0747620:three_prime_UTR 55.0%
!!! CGCTTTTTCGGCTTCGCCTA+AGG - chr5_2:415609-415628 Msa0747620:three_prime_UTR 55.0%
! GGCGTCGAGTGATTCAAGAG+CGG - chr5_2:415708-415727 Msa0747620:three_prime_UTR 55.0%
TCGCTTGAGCAAGGTGAACG+TGG + chr5_2:415752-415771 Msa0747630:intron 55.0%
! TGAACGCGGACGAGTTGACT+CGG + chr5_2:415817-415836 Msa0747630:CDS 55.0%
!! ACGAGTTGACTCGGCGTTAG+CGG + chr5_2:415826-415845 Msa0747630:CDS 55.0%
GTCCGAAGAACGCGAAAGCT+TGG + chr5_2:415873-415892 Msa0747630:CDS 55.0%
!! GCGAAAGCTTGGTGTGCGTT+GGG + chr5_2:415884-415903 Msa0747630:CDS 55.0%
GTCATGTTAACCGGTGCCTC+CGG + chr5_2:417435-417454 Msa0747630:intron 55.0%
TCATGTTAACCGGTGCCTCC+GGG + chr5_2:417436-417455 Msa0747630:intron 55.0%
! AAACAATGTCCCGGAGGCAC+CGG - chr5_2:417448-417467 Msa0747620:intron 55.0%
!! AATGTCCGGGGCACTGGTTA+AGG - chr5_2:417580-417599 Msa0747620:intron 55.0%
TTAACCAGTGCCCCGGACAT+TGG + chr5_2:417579-417598 Msa0747630:intron 55.0%
TTAACCAATGTCCGGGGCAC+TGG - chr5_2:417586-417605 Msa0747620:intron 55.0%
!! CGGTGTTTCTTGGCTTTGGC+AGG + chr5_2:418224-418243 Msa0747630:three_prime_UTR 55.0%
CGCCTAAGGCTTCGTTTGCG+AGG - chr5_2:415595-415614 Msa0747620:three_prime_UTR 60.0%
ACCTTGCTCAAGCGAACCGG+CGG - chr5_2:415747-415766 Msa0747620:three_prime_UTR 60.0%
!! CGCGAAAGCTTGGTGTGCGT+TGG + chr5_2:415883-415902 Msa0747630:CDS 60.0%
GGCGGCGAGAGGAGACAACG+CGG - chr5_2:415729-415748 Msa0747620:three_prime_UTR 70.0%
GCGTTGTCTCCTCTCGCCGC+CGG + chr5_2:415728-415747 Msa0747630:CDS 70.0%
TCAAGCGAACCGGCGGCGAG+AGG - chr5_2:415740-415759 Msa0747620:three_prime_UTR 70.0%
!! GCCGCCGGTTCGCTTGAGCA+AGG + chr5_2:415743-415762 Msa0747630:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 415359 418446 415359 ID=Msa0747630;Name=Msa0747630
chr5_2 mRNA 415359 418446 415359 ID=Msa0747630-mRNA-1;Parent=Msa0747630;Name=Msa0747630-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.33|0.25|1|0|0|4|286|217
chr5_2 exon 415359 415523 415359 ID=Msa0747630-mRNA-1:exon:47;Parent=Msa0747630-mRNA-1
chr5_2 exon 415539 415749 415539 ID=Msa0747630-mRNA-1:exon:48;Parent=Msa0747630-mRNA-1
chr5_2 exon 415765 416011 415765 ID=Msa0747630-mRNA-1:exon:49;Parent=Msa0747630-mRNA-1
chr5_2 exon 418130 418446 418130 ID=Msa0747630-mRNA-1:exon:50;Parent=Msa0747630-mRNA-1
chr5_2 CDS 415359 415523 415359 ID=Msa0747630-mRNA-1:cds;Parent=Msa0747630-mRNA-1
chr5_2 CDS 415539 415749 415539 ID=Msa0747630-mRNA-1:cds;Parent=Msa0747630-mRNA-1
chr5_2 CDS 415765 416011 415765 ID=Msa0747630-mRNA-1:cds;Parent=Msa0747630-mRNA-1
chr5_2 CDS 418130 418160 418130 ID=Msa0747630-mRNA-1:cds;Parent=Msa0747630-mRNA-1
chr5_2 three_prime_UTR 418161 418446 418161 ID=Msa0747630-mRNA-1:three_prime_utr;Parent=Msa0747630-mRNA-1
Gene Sequence

>Msa0747630

ATGGAGATCACTGAGAAACAAGCGATGTACCGCGACATCGGTTTACAAGTCATCATGATCCTCGCAATGGCTTTAATCTTCCTCAGCATGCACGGAATTCCACAAAAATTCCTCGCTAAACTCCGATTCCAAAACCGAAAAGCAATCAAAGCAAAGCGTCACTTCCTTCTCACACAAGCTAGATCTTCCAAAAACAAATCCAGATCCGCCATCAACAACCTCGCAAACGAAGCCTTAGGCGAAGCCGAAAAAGCGATCGAACTCGATCCAAAAGACGCCGCTTCGTATCTTCTCAAAGCTATGATTCTCGATCTTCAAGGTTTTCGTACTTCCGCTCTTGAATCACTCGACGCCGCGTTGTCTCCTCTCGCCGCCGGTGAACGTGGCGATGCGTTGTTTAAGAGAGCTGAGTTGAAACTCGCAACGAGTGAACGCGGACGAGTTGACTCGGCGTTAGCGGATTTGACTGAGTCGGTGATTTTGAGTCCGAAGAACGCGAAAGCTTGGTGTGCGTTGGGAGAGTGTTATGAAGGGAAGAAAATGGATGAGGAAGCAAAGAAAGCTTATAAGGAAGCTCATGAATTGGCGCCACAGTATTCTGTGCCTGTAGAAGCACTTAACAGCAATGTAACAAAAGATACGGAGCAGTCTTGA
Protein sequence

>Msa0747630

MEITEKQAMYRDIGLQVIMILAMALIFLSMHGIPQKFLAKLRFQNRKAIKAKRHFLLTQARSSKNKSRSAINNLANEALGEAEKAIELDPKDAASYLLKAMILDLQGFRTSALESLDAALSPLAAGERGDALFKRAELKLATSERGRVDSALADLTESVILSPKNAKAWCALGECYEGKKMDEEAKKAYKEAHELAPQYSVPVEALNSNVTKDTEQS*