Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401140 | XP_003597774.1 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 617 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401140 | sp|Q9S7T5|OFP14_ARATH | 36.025 | 322 | 145 | 10 | 1 | 295 | 1 | 288 | 1.15e-45 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401140 | G7II51 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 617 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1401140 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003560 | Msa1401140 | 0.809471 | 1.861816e-50 | -8.615850e-47 |
| Msa0029850 | Msa1401140 | 0.810537 | 1.097358e-50 | -8.615850e-47 |
| Msa0042550 | Msa1401140 | 0.826194 | 3.100210e-54 | -8.615850e-47 |
| Msa0048000 | Msa1401140 | 0.850261 | 1.904287e-60 | -8.615850e-47 |
| Msa0085720 | Msa1401140 | 0.818568 | 1.831111e-52 | -8.615850e-47 |
| Msa0101880 | Msa1401140 | 0.803855 | 2.855788e-49 | -8.615850e-47 |
| Msa1352540 | Msa1401140 | -0.801058 | 1.076924e-48 | -8.615850e-47 |
| Msa1372150 | Msa1401140 | 0.826270 | 2.974549e-54 | -8.615850e-47 |
| Msa1373350 | Msa1401140 | 0.807754 | 4.330969e-50 | -8.615850e-47 |
| Msa1376890 | Msa1401140 | 0.824514 | 7.746583e-54 | -8.615850e-47 |
| Msa1379850 | Msa1401140 | 0.809994 | 1.436897e-50 | -8.615850e-47 |
| Msa1388450 | Msa1401140 | 0.806024 | 1.005691e-49 | -8.615850e-47 |
| Msa1390120 | Msa1401140 | 0.800116 | 1.675756e-48 | -8.615850e-47 |
| Msa1397260 | Msa1401140 | 0.992567 | 4.516048e-194 | -8.615850e-47 |
| Msa1400070 | Msa1401140 | 0.800849 | 1.188231e-48 | -8.615850e-47 |
| Msa1401140 | Msa1401530 | 0.802535 | 5.358167e-49 | -8.615850e-47 |
| Msa1401140 | Msa1404540 | 0.840976 | 6.283769e-58 | -8.615850e-47 |
| Msa1401140 | Msa1404670 | 0.817067 | 3.995839e-52 | -8.615850e-47 |
| Msa1401140 | Msa1408790 | 0.808423 | 3.120226e-50 | -8.615850e-47 |
| Msa1401140 | Msa1424680 | 0.810681 | 1.021250e-50 | -8.615850e-47 |
| Msa1401140 | Msa1431060 | 0.849141 | 3.912048e-60 | -8.615850e-47 |
| Msa1401140 | Msa1435400 | 0.849739 | 2.665839e-60 | -8.615850e-47 |
| Msa1401140 | Msa1436040 | 0.815038 | 1.134657e-51 | -8.615850e-47 |
| Msa1401140 | Msa1440940 | 0.837223 | 5.901869e-57 | -8.615850e-47 |
| Msa1401140 | Msa1445220 | 0.800577 | 1.350226e-48 | -8.615850e-47 |
| Msa1401140 | Msa1455390 | 0.815490 | 8.999847e-52 | -8.615850e-47 |
| Msa1401140 | Msa1463650 | -0.802397 | 5.720550e-49 | -8.615850e-47 |
| Msa0613460 | Msa1401140 | 0.826238 | 3.026525e-54 | -8.615850e-47 |
| Msa0618670 | Msa1401140 | 0.801407 | 9.135185e-49 | -8.615850e-47 |
| Msa0626280 | Msa1401140 | 0.809726 | 1.640631e-50 | -8.615850e-47 |
| Msa0626990 | Msa1401140 | 0.813522 | 2.453114e-51 | -8.615850e-47 |
| Msa0627350 | Msa1401140 | 0.819421 | 1.172021e-52 | -8.615850e-47 |
| Msa0629630 | Msa1401140 | 0.818116 | 2.318003e-52 | -8.615850e-47 |
| Msa0651270 | Msa1401140 | 0.800225 | 1.592303e-48 | -8.615850e-47 |
| Msa0658570 | Msa1401140 | 0.815408 | 9.388370e-52 | -8.615850e-47 |
| Msa0677080 | Msa1401140 | 0.803739 | 3.018484e-49 | -8.615850e-47 |
| Msa0679550 | Msa1401140 | 0.810407 | 1.170673e-50 | -8.615850e-47 |
| Msa0693380 | Msa1401140 | 0.812056 | 5.135825e-51 | -8.615850e-47 |
| Msa0696410 | Msa1401140 | 0.817910 | 2.581264e-52 | -8.615850e-47 |
| Msa0703490 | Msa1401140 | 0.804032 | 2.623896e-49 | -8.615850e-47 |
| Msa0715110 | Msa1401140 | 0.805575 | 1.249248e-49 | -8.615850e-47 |
| Msa0724560 | Msa1401140 | 0.801739 | 7.809421e-49 | -8.615850e-47 |
| Msa0724950 | Msa1401140 | 0.805069 | 1.594955e-49 | -8.615850e-47 |
| Msa0731470 | Msa1401140 | 0.823478 | 1.355917e-53 | -8.615850e-47 |
| Msa0735160 | Msa1401140 | 0.821498 | 3.910839e-53 | -8.615850e-47 |
| Msa0755070 | Msa1401140 | 0.810727 | 9.982060e-51 | -8.615850e-47 |
| Msa0757050 | Msa1401140 | 0.807680 | 4.491485e-50 | -8.615850e-47 |
| Msa0757080 | Msa1401140 | 0.828006 | 1.142583e-54 | -8.615850e-47 |
| Msa0763080 | Msa1401140 | 0.812028 | 5.209208e-51 | -8.615850e-47 |
| Msa0766090 | Msa1401140 | 0.806450 | 8.176998e-50 | -8.615850e-47 |
| Msa0773310 | Msa1401140 | 0.845753 | 3.335718e-59 | -8.615850e-47 |
| Msa0796960 | Msa1401140 | 0.803426 | 3.506492e-49 | -8.615850e-47 |
| Msa0798030 | Msa1401140 | 0.804570 | 2.027165e-49 | -8.615850e-47 |
| Msa0809740 | Msa1401140 | 0.876436 | 1.398448e-68 | -8.615850e-47 |
| Msa0810760 | Msa1401140 | 0.834929 | 2.255890e-56 | -8.615850e-47 |
| Msa0818110 | Msa1401140 | 0.849176 | 3.825023e-60 | -8.615850e-47 |
| Msa0831150 | Msa1401140 | 0.800860 | 1.182265e-48 | -8.615850e-47 |
| Msa0834440 | Msa1401140 | 0.811227 | 7.779321e-51 | -8.615850e-47 |
| Msa0124550 | Msa1401140 | 0.818676 | 1.730900e-52 | -8.615850e-47 |
| Msa0169820 | Msa1401140 | 0.810560 | 1.085052e-50 | -8.615850e-47 |
| Msa0178510 | Msa1401140 | 0.833525 | 5.073216e-56 | -8.615850e-47 |
| Msa0206790 | Msa1401140 | 0.805381 | 1.372261e-49 | -8.615850e-47 |
| Msa0223380 | Msa1401140 | 0.803739 | 3.018484e-49 | -8.615850e-47 |
| Msa1247030 | Msa1401140 | 0.821371 | 4.184376e-53 | -8.615850e-47 |
| Msa1262990 | Msa1401140 | -0.802348 | 5.854680e-49 | -8.615850e-47 |
| Msa1264860 | Msa1401140 | -0.830548 | 2.760342e-55 | -8.615850e-47 |
| Msa1267060 | Msa1401140 | 0.810823 | 9.517739e-51 | -8.615850e-47 |
| Msa1299070 | Msa1401140 | 0.837365 | 5.425277e-57 | -8.615850e-47 |
| Msa1335710 | Msa1401140 | 0.820897 | 5.379513e-53 | -8.615850e-47 |
| Msa1338250 | Msa1401140 | 0.802772 | 4.788213e-49 | -8.615850e-47 |
| Msa0365490 | Msa1401140 | 0.807744 | 4.352502e-50 | -8.615850e-47 |
| Msa0377530 | Msa1401140 | 0.834690 | 2.590009e-56 | -8.615850e-47 |
| Msa0378380 | Msa1401140 | 0.803296 | 3.729971e-49 | -8.615850e-47 |
| Msa0388030 | Msa1401140 | -0.857644 | 1.418165e-62 | -8.615850e-47 |
| Msa0392040 | Msa1401140 | -0.808575 | 2.895966e-50 | -8.615850e-47 |
| Msa0422500 | Msa1401140 | 0.813957 | 1.967519e-51 | -8.615850e-47 |
| Msa0436370 | Msa1401140 | 0.809306 | 2.019306e-50 | -8.615850e-47 |
| Msa0445280 | Msa1401140 | 0.809465 | 1.867189e-50 | -8.615850e-47 |
| Msa0450350 | Msa1401140 | 0.820177 | 7.870195e-53 | -8.615850e-47 |
| Msa0458440 | Msa1401140 | 0.813759 | 2.175415e-51 | -8.615850e-47 |
| Msa0462630 | Msa1401140 | 0.804097 | 2.544420e-49 | -8.615850e-47 |
| Msa0464900 | Msa1401140 | 0.848430 | 6.160710e-60 | -8.615850e-47 |
| Msa0469940 | Msa1401140 | -0.818939 | 1.508793e-52 | -8.615850e-47 |
| Msa0473240 | Msa1401140 | 0.823274 | 1.513198e-53 | -8.615850e-47 |
| Msa0480600 | Msa1401140 | 0.816705 | 4.819109e-52 | -8.615850e-47 |
| Msa1006850 | Msa1401140 | 0.824838 | 6.497870e-54 | -8.615850e-47 |
| Msa1021510 | Msa1401140 | 0.853667 | 2.053096e-61 | -8.615850e-47 |
| Msa1028270 | Msa1401140 | 0.817356 | 3.440327e-52 | -8.615850e-47 |
| Msa1037650 | Msa1401140 | -0.800662 | 1.297389e-48 | -8.615850e-47 |
| Msa1040520 | Msa1401140 | 0.802448 | 5.584276e-49 | -8.615850e-47 |
| Msa1068200 | Msa1401140 | -0.812830 | 3.480379e-51 | -8.615850e-47 |
| Msa1080160 | Msa1401140 | 0.837660 | 4.560526e-57 | -8.615850e-47 |
| Msa1082500 | Msa1401140 | 0.814111 | 1.819224e-51 | -8.615850e-47 |
| Msa1084260 | Msa1401140 | 0.804693 | 1.911347e-49 | -8.615850e-47 |
| Msa1087470 | Msa1401140 | 0.810536 | 1.097702e-50 | -8.615850e-47 |
| Msa1100480 | Msa1401140 | 0.820719 | 5.911864e-53 | -8.615850e-47 |
| Msa1105500 | Msa1401140 | 0.847976 | 8.223456e-60 | -8.615850e-47 |
| Msa0260810 | Msa1401140 | 0.844223 | 8.637437e-59 | -8.615850e-47 |
| Msa0266000 | Msa1401140 | -0.817351 | 3.449323e-52 | -8.615850e-47 |
| Msa0268090 | Msa1401140 | 0.815029 | 1.139781e-51 | -8.615850e-47 |
| Msa0276920 | Msa1401140 | 0.800141 | 1.656515e-48 | -8.615850e-47 |
| Msa0288320 | Msa1401140 | 0.808885 | 2.485646e-50 | -8.615850e-47 |
| Msa0292170 | Msa1401140 | 0.812196 | 4.787694e-51 | -8.615850e-47 |
| Msa0297960 | Msa1401140 | 0.816012 | 6.887399e-52 | -8.615850e-47 |
| Msa0308550 | Msa1401140 | 0.801860 | 7.375559e-49 | -8.615850e-47 |
| Msa0330390 | Msa1401140 | 0.808347 | 3.239110e-50 | -8.615850e-47 |
| Msa0335040 | Msa1401140 | 0.823545 | 1.307756e-53 | -8.615850e-47 |
| Msa0344870 | Msa1401140 | 0.804462 | 2.135624e-49 | -8.615850e-47 |
| Msa0350840 | Msa1401140 | 0.803533 | 3.332142e-49 | -8.615850e-47 |
| Msa0356890 | Msa1401140 | -0.801695 | 7.976157e-49 | -8.615850e-47 |
| Msa1118760 | Msa1401140 | 0.820441 | 6.849225e-53 | -8.615850e-47 |
| Msa1127770 | Msa1401140 | 0.807240 | 5.567661e-50 | -8.615850e-47 |
| Msa1135880 | Msa1401140 | 0.807134 | 5.862810e-50 | -8.615850e-47 |
| Msa1144720 | Msa1401140 | -0.809728 | 1.639098e-50 | -8.615850e-47 |
| Msa1152210 | Msa1401140 | 0.805666 | 1.195464e-49 | -8.615850e-47 |
| Msa1152950 | Msa1401140 | 0.817596 | 3.037744e-52 | -8.615850e-47 |
| Msa1156340 | Msa1401140 | -0.803330 | 3.671096e-49 | -8.615850e-47 |
| Msa1158660 | Msa1401140 | 0.812044 | 5.166577e-51 | -8.615850e-47 |
| Msa1171730 | Msa1401140 | 0.812681 | 3.751037e-51 | -8.615850e-47 |
| Msa1176270 | Msa1401140 | 0.805382 | 1.371753e-49 | -8.615850e-47 |
| Msa1205390 | Msa1401140 | 0.800207 | 1.606268e-48 | -8.615850e-47 |
| Msa1219810 | Msa1401140 | 0.804920 | 1.713272e-49 | -8.615850e-47 |
| Msa0484780 | Msa1401140 | 0.829026 | 6.480960e-55 | -8.615850e-47 |
| Msa0511250 | Msa1401140 | 0.806914 | 6.526274e-50 | -8.615850e-47 |
| Msa0517490 | Msa1401140 | 0.846637 | 1.916204e-59 | -8.615850e-47 |
| Msa0530650 | Msa1401140 | 0.823255 | 1.527996e-53 | -8.615850e-47 |
| Msa0532540 | Msa1401140 | 0.800662 | 1.297389e-48 | -8.615850e-47 |
| Msa0558690 | Msa1401140 | 0.804052 | 2.599494e-49 | -8.615850e-47 |
| Msa0567020 | Msa1401140 | 0.824924 | 6.199257e-54 | -8.615850e-47 |
| Msa0572480 | Msa1401140 | 0.811179 | 7.970013e-51 | -8.615850e-47 |
| Msa0580000 | Msa1401140 | 0.801176 | 1.018530e-48 | -8.615850e-47 |
| Msa0587190 | Msa1401140 | 0.813442 | 2.554952e-51 | -8.615850e-47 |
| Msa0589390 | Msa1401140 | 0.800159 | 1.642682e-48 | -8.615850e-47 |
| Msa0598160 | Msa1401140 | 0.800083 | 1.701845e-48 | -8.615850e-47 |
| Msa0845070 | Msa1401140 | 0.814657 | 1.378241e-51 | -8.615850e-47 |
| Msa0850240 | Msa1401140 | 0.826975 | 2.019134e-54 | -8.615850e-47 |
| Msa0870530 | Msa1401140 | 0.809583 | 1.760858e-50 | -8.615850e-47 |
| Msa0908170 | Msa1401140 | 0.834687 | 2.595108e-56 | -8.615850e-47 |
| Msa0936210 | Msa1401140 | 0.809171 | 2.158726e-50 | -8.615850e-47 |
| Msa0944730 | Msa1401140 | 0.820958 | 5.208175e-53 | -8.615850e-47 |
| Msa0973150 | Msa1401140 | 0.801125 | 1.043643e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401140 | MtrunA17_Chr2g0331871 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 617 |
| Msa1401140 | MtrunA17_Chr4g0004341 | 47.003 | 317 | 130 | 8 | 1 | 306 | 1 | 290 | 2.15e-76 | 235 |
| Msa1401140 | MtrunA17_Chr1g0185481 | 38.406 | 138 | 61 | 4 | 137 | 268 | 147 | 266 | 6.24e-18 | 82.0 |
| Msa1401140 | MtrunA17_Chr7g0259991 | 47.887 | 71 | 35 | 1 | 198 | 268 | 132 | 200 | 3.46e-15 | 73.2 |
| Msa1401140 | MtrunA17_Chr1g0210351 | 28.689 | 244 | 96 | 9 | 29 | 265 | 26 | 198 | 1.96e-13 | 68.9 |
| Msa1401140 | MtrunA17_Chr1g0205341 | 42.188 | 64 | 34 | 1 | 203 | 266 | 117 | 177 | 4.08e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401140 | AT1G79960.1 | 36.025 | 322 | 145 | 10 | 1 | 295 | 1 | 288 | 1.17e-46 | 159 |
| Msa1401140 | AT5G04820.1 | 35.338 | 133 | 62 | 4 | 134 | 266 | 102 | 210 | 2.75e-14 | 72.0 |
| Msa1401140 | AT4G14860.1 | 45.312 | 64 | 28 | 2 | 204 | 265 | 109 | 167 | 2.29e-11 | 62.4 |
Find 49 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCAGGTTACCATAGAATT+TGG | 0.233067 | tig0022500:-20225 | Msa1401140:CDS |
| GAAAATTGATTGGGACTTCA+TGG | 0.309098 | tig0022500:-20421 | Msa1401140:CDS |
| TTTGCAGCCAGAGAGCATTT+TGG | 0.332403 | tig0022500:+21031 | None:intergenic |
| TAAAGAGGAACCAAATTCTA+TGG | 0.379032 | tig0022500:+20215 | None:intergenic |
| CAGGAACCACCAATACGTTT+TGG | 0.387081 | tig0022500:+20789 | None:intergenic |
| ACAATCAAAGGAAAATTGAT+TGG | 0.389239 | tig0022500:-20431 | Msa1401140:CDS |
| GTTAATCGAATTTGAGGCTG+TGG | 0.438345 | tig0022500:+21053 | None:intergenic |
| TATTGGTGGTTCCTGGTTGT+TGG | 0.439269 | tig0022500:-20781 | Msa1401140:CDS |
| TTAGGATTGGTAGGGAAGTC+AGG | 0.440786 | tig0022500:-20260 | Msa1401140:CDS |
| CAAAACGTATTGGTGGTTCC+TGG | 0.454127 | tig0022500:-20788 | Msa1401140:CDS |
| GAGTTGGTGGAATCAAAGTA+TGG | 0.465659 | tig0022500:-20465 | Msa1401140:CDS |
| TTGATCTCATCACTGTTATG+AGG | 0.467638 | tig0022500:-20329 | Msa1401140:CDS |
| GAAGAATTCAGAGGCTGCTC+CGG | 0.469764 | tig0022500:-20307 | Msa1401140:CDS |
| CGATTAACCAAAATGCTCTC+TGG | 0.470779 | tig0022500:-21038 | Msa1401140:CDS |
| CGTGTAGCGTGAGAACTGTT+AGG | 0.472831 | tig0022500:-20278 | Msa1401140:CDS |
| AACAGTGCAACACAATTCTC+AGG | 0.479484 | tig0022500:+20534 | None:intergenic |
| AGGTAACTCAGGATCATTGA+TGG | 0.483746 | tig0022500:-20691 | Msa1401140:CDS |
| ACACCACCTCAAGATCTTTG+TGG | 0.490451 | tig0022500:-20738 | Msa1401140:CDS |
| CAATCAAAGGAAAATTGATT+GGG | 0.491479 | tig0022500:-20430 | Msa1401140:CDS |
| TGAAATTCTCAAAGAGAAAA+CGG | 0.493044 | tig0022500:+20904 | None:intergenic |
| ATCAATGATCCTGAGTTACC+TGG | 0.499280 | tig0022500:+20693 | None:intergenic |
| AACAGCATCACCTTCAAAAG+AGG | 0.511692 | tig0022500:-20598 | Msa1401140:CDS |
| TGAGAACTGTTAGGATTGGT+AGG | 0.512241 | tig0022500:-20269 | Msa1401140:CDS |
| GCGATCAATGCAAGAGTTGG+TGG | 0.513161 | tig0022500:-20478 | Msa1401140:CDS |
| CTGAAATCGTCGTAAGGACT+CGG | 0.524949 | tig0022500:+20501 | None:intergenic |
| TTTGTTGAGGTAATCTTGAA+GGG | 0.525082 | tig0022500:+21091 | None:intergenic |
| CAGGCGATCAATGCAAGAGT+TGG | 0.529425 | tig0022500:-20481 | Msa1401140:CDS |
| AAATTCTATGGTAACCTGCT+TGG | 0.538786 | tig0022500:+20227 | None:intergenic |
| CTCACGCTACACGGCTTCGC+CGG | 0.538841 | tig0022500:+20288 | None:intergenic |
| TTTGTTGTTAACGGCGTTTG+AGG | 0.539359 | tig0022500:+20959 | None:intergenic |
| GAGGAACCACCAAAACGTAT+TGG | 0.549662 | tig0022500:-20798 | Msa1401140:CDS |
| GCACGATTCTTCGTGAAACC+AGG | 0.558059 | tig0022500:-20711 | Msa1401140:CDS |
| TATGTTTCTGCCGTTATCCA+AGG | 0.558329 | tig0022500:+20986 | None:intergenic |
| CTAACAGTTCTCACGCTACA+CGG | 0.576486 | tig0022500:+20279 | None:intergenic |
| TTAATCGAATTTGAGGCTGT+GGG | 0.576513 | tig0022500:+21054 | None:intergenic |
| TTCGTGAAACCAGGTAACTC+AGG | 0.588008 | tig0022500:-20702 | Msa1401140:CDS |
| AGCGTGAGAACTGTTAGGAT+TGG | 0.588361 | tig0022500:-20273 | Msa1401140:CDS |
| CAGGAAGAAGAAGACCAAGC+AGG | 0.606262 | tig0022500:-20241 | Msa1401140:CDS |
| TCTCGGTGACTCCAACAACC+AGG | 0.608261 | tig0022500:+20770 | None:intergenic |
| GTAAGATTGAAAACAATCAA+AGG | 0.608557 | tig0022500:-20443 | Msa1401140:CDS |
| GAACCACCAAAACGTATTGG+TGG | 0.614019 | tig0022500:-20795 | Msa1401140:CDS |
| GCTGAGCCACAAAGATCTTG+AGG | 0.618030 | tig0022500:+20732 | None:intergenic |
| TGTGATGATTGTGATCATCA+TGG | 0.625055 | tig0022500:+20562 | None:intergenic |
| GATCGCCTGAAATCGTCGTA+AGG | 0.629682 | tig0022500:+20495 | None:intergenic |
| GAGAACTGTTAGGATTGGTA+GGG | 0.631326 | tig0022500:-20268 | Msa1401140:CDS |
| GGTGGTGTTGTAATGAATCT+CGG | 0.636568 | tig0022500:+20753 | None:intergenic |
| AATTGATTGGGACTTCATGG+AGG | 0.677814 | tig0022500:-20418 | Msa1401140:CDS |
| TGTTATGAGGAAGAATTCAG+AGG | 0.682074 | tig0022500:-20316 | Msa1401140:CDS |
| GAGCCACAAAGATCTTGAGG+TGG | 0.753269 | tig0022500:+20735 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAACTAAAG+AGG | + | tig0022500:21104-21123 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAAAACTAAAG+AGG | + | tig0022500:21104-21123 | None:intergenic | 10.0% |
| !!! | TTTTGTTTTTGATTTTGTTG+AGG | + | tig0022500:20226-20245 | None:intergenic | 20.0% |
| !!! | ATCATTGATTTTGTTGTTAA+CGG | + | tig0022500:20354-20373 | None:intergenic | 20.0% |
| !!! | TTTTGTTTTTGATTTTGTTG+AGG | + | tig0022500:20226-20245 | None:intergenic | 20.0% |
| !!! | ATCATTGATTTTGTTGTTAA+CGG | + | tig0022500:20354-20373 | None:intergenic | 20.0% |
| !!! | ATTTTGGTTAATCGAATTTG+AGG | + | tig0022500:20257-20276 | None:intergenic | 25.0% |
| ! | TGAAATTCTCAAAGAGAAAA+CGG | + | tig0022500:20400-20419 | None:intergenic | 25.0% |
| ! | CAACAAAAACAAAAGAATCT+CGG | - | tig0022500:20466-20485 | Msa1401140:CDS | 25.0% |
| ! | GTAAGATTGAAAACAATCAA+AGG | - | tig0022500:20858-20877 | Msa1401140:CDS | 25.0% |
| ! | ACAATCAAAGGAAAATTGAT+TGG | - | tig0022500:20870-20889 | Msa1401140:CDS | 25.0% |
| ! | CAATCAAAGGAAAATTGATT+GGG | - | tig0022500:20871-20890 | Msa1401140:CDS | 25.0% |
| !!! | ATTTTGGTTAATCGAATTTG+AGG | + | tig0022500:20257-20276 | None:intergenic | 25.0% |
| ! | TGAAATTCTCAAAGAGAAAA+CGG | + | tig0022500:20400-20419 | None:intergenic | 25.0% |
| ! | CAACAAAAACAAAAGAATCT+CGG | - | tig0022500:20466-20485 | Msa1401140:CDS | 25.0% |
| ! | GTAAGATTGAAAACAATCAA+AGG | - | tig0022500:20858-20877 | Msa1401140:CDS | 25.0% |
| ! | ACAATCAAAGGAAAATTGAT+TGG | - | tig0022500:20870-20889 | Msa1401140:CDS | 25.0% |
| ! | CAATCAAAGGAAAATTGATT+GGG | - | tig0022500:20871-20890 | Msa1401140:CDS | 25.0% |
| TTTGTTGAGGTAATCTTGAA+GGG | + | tig0022500:20213-20232 | None:intergenic | 30.0% | |
| ! | TTTTGTTGAGGTAATCTTGA+AGG | + | tig0022500:20214-20233 | None:intergenic | 30.0% |
| ! | AAGGAAAAAGATGGTGTTTT+GGG | + | tig0022500:20299-20318 | None:intergenic | 30.0% |
| ! | ACTACAATAACCTCTTTTGA+AGG | + | tig0022500:20716-20735 | None:intergenic | 30.0% |
| TAAAGAGGAACCAAATTCTA+TGG | + | tig0022500:21089-21108 | None:intergenic | 30.0% | |
| TTTGTTGAGGTAATCTTGAA+GGG | + | tig0022500:20213-20232 | None:intergenic | 30.0% | |
| ! | TTTTGTTGAGGTAATCTTGA+AGG | + | tig0022500:20214-20233 | None:intergenic | 30.0% |
| ! | AAGGAAAAAGATGGTGTTTT+GGG | + | tig0022500:20299-20318 | None:intergenic | 30.0% |
| ! | ACTACAATAACCTCTTTTGA+AGG | + | tig0022500:20716-20735 | None:intergenic | 30.0% |
| TAAAGAGGAACCAAATTCTA+TGG | + | tig0022500:21089-21108 | None:intergenic | 30.0% | |
| !!! | AGGGTTTTTTGAAGCTTCTT+AGG | + | tig0022500:20194-20213 | None:intergenic | 35.0% |
| ! | TTAATCGAATTTGAGGCTGT+GGG | + | tig0022500:20250-20269 | None:intergenic | 35.0% |
| ! | CAAGGAAAAAGATGGTGTTT+TGG | + | tig0022500:20300-20319 | None:intergenic | 35.0% |
| ! | CAAAACACCATCTTTTTCCT+TGG | - | tig0022500:20298-20317 | Msa1401140:CDS | 35.0% |
| ! | CCATCTTTTTCCTTGGATAA+CGG | - | tig0022500:20305-20324 | Msa1401140:CDS | 35.0% |
| TGTGATGATTGTGATCATCA+TGG | + | tig0022500:20742-20761 | None:intergenic | 35.0% | |
| GAAAATTGATTGGGACTTCA+TGG | - | tig0022500:20880-20899 | Msa1401140:CDS | 35.0% | |
| TTGATCTCATCACTGTTATG+AGG | - | tig0022500:20972-20991 | Msa1401140:CDS | 35.0% | |
| TGTTATGAGGAAGAATTCAG+AGG | - | tig0022500:20985-21004 | Msa1401140:CDS | 35.0% | |
| AAATTCTATGGTAACCTGCT+TGG | + | tig0022500:21077-21096 | None:intergenic | 35.0% | |
| AAGCAGGTTACCATAGAATT+TGG | - | tig0022500:21076-21095 | Msa1401140:CDS | 35.0% | |
| !!! | AGGGTTTTTTGAAGCTTCTT+AGG | + | tig0022500:20194-20213 | None:intergenic | 35.0% |
| ! | TTAATCGAATTTGAGGCTGT+GGG | + | tig0022500:20250-20269 | None:intergenic | 35.0% |
| ! | CAAGGAAAAAGATGGTGTTT+TGG | + | tig0022500:20300-20319 | None:intergenic | 35.0% |
| ! | CAAAACACCATCTTTTTCCT+TGG | - | tig0022500:20298-20317 | Msa1401140:CDS | 35.0% |
| ! | CCATCTTTTTCCTTGGATAA+CGG | - | tig0022500:20305-20324 | Msa1401140:CDS | 35.0% |
| TGTGATGATTGTGATCATCA+TGG | + | tig0022500:20742-20761 | None:intergenic | 35.0% | |
| GAAAATTGATTGGGACTTCA+TGG | - | tig0022500:20880-20899 | Msa1401140:CDS | 35.0% | |
| TTGATCTCATCACTGTTATG+AGG | - | tig0022500:20972-20991 | Msa1401140:CDS | 35.0% | |
| TGTTATGAGGAAGAATTCAG+AGG | - | tig0022500:20985-21004 | Msa1401140:CDS | 35.0% | |
| AAATTCTATGGTAACCTGCT+TGG | + | tig0022500:21077-21096 | None:intergenic | 35.0% | |
| AAGCAGGTTACCATAGAATT+TGG | - | tig0022500:21076-21095 | Msa1401140:CDS | 35.0% | |
| ! | GTTAATCGAATTTGAGGCTG+TGG | + | tig0022500:20251-20270 | None:intergenic | 40.0% |
| CGATTAACCAAAATGCTCTC+TGG | - | tig0022500:20263-20282 | Msa1401140:CDS | 40.0% | |
| CCGTTATCCAAGGAAAAAGA+TGG | + | tig0022500:20308-20327 | None:intergenic | 40.0% | |
| ! | TATGTTTCTGCCGTTATCCA+AGG | + | tig0022500:20318-20337 | None:intergenic | 40.0% |
| !! | TTTGTTGTTAACGGCGTTTG+AGG | + | tig0022500:20345-20364 | None:intergenic | 40.0% |
| CTCGGATGAAAAAAACAACG+AGG | - | tig0022500:20484-20503 | Msa1401140:CDS | 40.0% | |
| !! | GGTGGTGTTGTAATGAATCT+CGG | + | tig0022500:20551-20570 | None:intergenic | 40.0% |
| ATCAATGATCCTGAGTTACC+TGG | + | tig0022500:20611-20630 | None:intergenic | 40.0% | |
| AGGTAACTCAGGATCATTGA+TGG | - | tig0022500:20610-20629 | Msa1401140:CDS | 40.0% | |
| AACAGCATCACCTTCAAAAG+AGG | - | tig0022500:20703-20722 | Msa1401140:CDS | 40.0% | |
| AACAGTGCAACACAATTCTC+AGG | + | tig0022500:20770-20789 | None:intergenic | 40.0% | |
| !! | GAGTTGGTGGAATCAAAGTA+TGG | - | tig0022500:20836-20855 | Msa1401140:CDS | 40.0% |
| AATTGATTGGGACTTCATGG+AGG | - | tig0022500:20883-20902 | Msa1401140:CDS | 40.0% | |
| ! | TGAGAACTGTTAGGATTGGT+AGG | - | tig0022500:21032-21051 | Msa1401140:CDS | 40.0% |
| ! | GAGAACTGTTAGGATTGGTA+GGG | - | tig0022500:21033-21052 | Msa1401140:CDS | 40.0% |
| ! | GTTAATCGAATTTGAGGCTG+TGG | + | tig0022500:20251-20270 | None:intergenic | 40.0% |
| CGATTAACCAAAATGCTCTC+TGG | - | tig0022500:20263-20282 | Msa1401140:CDS | 40.0% | |
| CCGTTATCCAAGGAAAAAGA+TGG | + | tig0022500:20308-20327 | None:intergenic | 40.0% | |
| ! | TATGTTTCTGCCGTTATCCA+AGG | + | tig0022500:20318-20337 | None:intergenic | 40.0% |
| !! | TTTGTTGTTAACGGCGTTTG+AGG | + | tig0022500:20345-20364 | None:intergenic | 40.0% |
| CTCGGATGAAAAAAACAACG+AGG | - | tig0022500:20484-20503 | Msa1401140:CDS | 40.0% | |
| !! | GGTGGTGTTGTAATGAATCT+CGG | + | tig0022500:20551-20570 | None:intergenic | 40.0% |
| ATCAATGATCCTGAGTTACC+TGG | + | tig0022500:20611-20630 | None:intergenic | 40.0% | |
| AGGTAACTCAGGATCATTGA+TGG | - | tig0022500:20610-20629 | Msa1401140:CDS | 40.0% | |
| AACAGCATCACCTTCAAAAG+AGG | - | tig0022500:20703-20722 | Msa1401140:CDS | 40.0% | |
| AACAGTGCAACACAATTCTC+AGG | + | tig0022500:20770-20789 | None:intergenic | 40.0% | |
| !! | GAGTTGGTGGAATCAAAGTA+TGG | - | tig0022500:20836-20855 | Msa1401140:CDS | 40.0% |
| AATTGATTGGGACTTCATGG+AGG | - | tig0022500:20883-20902 | Msa1401140:CDS | 40.0% | |
| ! | TGAGAACTGTTAGGATTGGT+AGG | - | tig0022500:21032-21051 | Msa1401140:CDS | 40.0% |
| ! | GAGAACTGTTAGGATTGGTA+GGG | - | tig0022500:21033-21052 | Msa1401140:CDS | 40.0% |
| !!! | TTTGCAGCCAGAGAGCATTT+TGG | + | tig0022500:20273-20292 | None:intergenic | 45.0% |
| GAGGAACCACCAAAACGTAT+TGG | - | tig0022500:20503-20522 | Msa1401140:CDS | 45.0% | |
| GAACCACCAAAACGTATTGG+TGG | - | tig0022500:20506-20525 | Msa1401140:CDS | 45.0% | |
| ! | GAACCACCAATACGTTTTGG+TGG | + | tig0022500:20512-20531 | None:intergenic | 45.0% |
| ! | CAGGAACCACCAATACGTTT+TGG | + | tig0022500:20515-20534 | None:intergenic | 45.0% |
| ! | CAAAACGTATTGGTGGTTCC+TGG | - | tig0022500:20513-20532 | Msa1401140:CDS | 45.0% |
| ! | TATTGGTGGTTCCTGGTTGT+TGG | - | tig0022500:20520-20539 | Msa1401140:CDS | 45.0% |
| ACACCACCTCAAGATCTTTG+TGG | - | tig0022500:20563-20582 | Msa1401140:CDS | 45.0% | |
| TTCGTGAAACCAGGTAACTC+AGG | - | tig0022500:20599-20618 | Msa1401140:CDS | 45.0% | |
| CTGAAATCGTCGTAAGGACT+CGG | + | tig0022500:20803-20822 | None:intergenic | 45.0% | |
| CTAACAGTTCTCACGCTACA+CGG | + | tig0022500:21025-21044 | None:intergenic | 45.0% | |
| AGCGTGAGAACTGTTAGGAT+TGG | - | tig0022500:21028-21047 | Msa1401140:CDS | 45.0% | |
| ! | TTAGGATTGGTAGGGAAGTC+AGG | - | tig0022500:21041-21060 | Msa1401140:CDS | 45.0% |
| !!! | TTTGCAGCCAGAGAGCATTT+TGG | + | tig0022500:20273-20292 | None:intergenic | 45.0% |
| GAGGAACCACCAAAACGTAT+TGG | - | tig0022500:20503-20522 | Msa1401140:CDS | 45.0% | |
| GAACCACCAAAACGTATTGG+TGG | - | tig0022500:20506-20525 | Msa1401140:CDS | 45.0% | |
| ! | GAACCACCAATACGTTTTGG+TGG | + | tig0022500:20512-20531 | None:intergenic | 45.0% |
| ! | CAGGAACCACCAATACGTTT+TGG | + | tig0022500:20515-20534 | None:intergenic | 45.0% |
| ! | CAAAACGTATTGGTGGTTCC+TGG | - | tig0022500:20513-20532 | Msa1401140:CDS | 45.0% |
| ! | TATTGGTGGTTCCTGGTTGT+TGG | - | tig0022500:20520-20539 | Msa1401140:CDS | 45.0% |
| ACACCACCTCAAGATCTTTG+TGG | - | tig0022500:20563-20582 | Msa1401140:CDS | 45.0% | |
| TTCGTGAAACCAGGTAACTC+AGG | - | tig0022500:20599-20618 | Msa1401140:CDS | 45.0% | |
| CTGAAATCGTCGTAAGGACT+CGG | + | tig0022500:20803-20822 | None:intergenic | 45.0% | |
| CTAACAGTTCTCACGCTACA+CGG | + | tig0022500:21025-21044 | None:intergenic | 45.0% | |
| AGCGTGAGAACTGTTAGGAT+TGG | - | tig0022500:21028-21047 | Msa1401140:CDS | 45.0% | |
| ! | TTAGGATTGGTAGGGAAGTC+AGG | - | tig0022500:21041-21060 | Msa1401140:CDS | 45.0% |
| GAGCCACAAAGATCTTGAGG+TGG | + | tig0022500:20569-20588 | None:intergenic | 50.0% | |
| GCTGAGCCACAAAGATCTTG+AGG | + | tig0022500:20572-20591 | None:intergenic | 50.0% | |
| GCACGATTCTTCGTGAAACC+AGG | - | tig0022500:20590-20609 | Msa1401140:CDS | 50.0% | |
| CGAGTCCTTACGACGATTTC+AGG | - | tig0022500:20801-20820 | Msa1401140:CDS | 50.0% | |
| GATCGCCTGAAATCGTCGTA+AGG | + | tig0022500:20809-20828 | None:intergenic | 50.0% | |
| CAGGCGATCAATGCAAGAGT+TGG | - | tig0022500:20820-20839 | Msa1401140:CDS | 50.0% | |
| GCGATCAATGCAAGAGTTGG+TGG | - | tig0022500:20823-20842 | Msa1401140:CDS | 50.0% | |
| GAAGAATTCAGAGGCTGCTC+CGG | - | tig0022500:20994-21013 | Msa1401140:CDS | 50.0% | |
| CGTGTAGCGTGAGAACTGTT+AGG | - | tig0022500:21023-21042 | Msa1401140:CDS | 50.0% | |
| CAGGAAGAAGAAGACCAAGC+AGG | - | tig0022500:21060-21079 | Msa1401140:CDS | 50.0% | |
| GAGCCACAAAGATCTTGAGG+TGG | + | tig0022500:20569-20588 | None:intergenic | 50.0% | |
| GCTGAGCCACAAAGATCTTG+AGG | + | tig0022500:20572-20591 | None:intergenic | 50.0% | |
| GCACGATTCTTCGTGAAACC+AGG | - | tig0022500:20590-20609 | Msa1401140:CDS | 50.0% | |
| CGAGTCCTTACGACGATTTC+AGG | - | tig0022500:20801-20820 | Msa1401140:CDS | 50.0% | |
| GATCGCCTGAAATCGTCGTA+AGG | + | tig0022500:20809-20828 | None:intergenic | 50.0% | |
| CAGGCGATCAATGCAAGAGT+TGG | - | tig0022500:20820-20839 | Msa1401140:CDS | 50.0% | |
| GCGATCAATGCAAGAGTTGG+TGG | - | tig0022500:20823-20842 | Msa1401140:CDS | 50.0% | |
| GAAGAATTCAGAGGCTGCTC+CGG | - | tig0022500:20994-21013 | Msa1401140:CDS | 50.0% | |
| CGTGTAGCGTGAGAACTGTT+AGG | - | tig0022500:21023-21042 | Msa1401140:CDS | 50.0% | |
| CAGGAAGAAGAAGACCAAGC+AGG | - | tig0022500:21060-21079 | Msa1401140:CDS | 50.0% | |
| TCTCGGTGACTCCAACAACC+AGG | + | tig0022500:20534-20553 | None:intergenic | 55.0% | |
| TCTCGGTGACTCCAACAACC+AGG | + | tig0022500:20534-20553 | None:intergenic | 55.0% | |
| CTCACGCTACACGGCTTCGC+CGG | + | tig0022500:21016-21035 | None:intergenic | 65.0% | |
| CTCACGCTACACGGCTTCGC+CGG | + | tig0022500:21016-21035 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022500 | gene | 20188 | 21135 | 20188 | ID=Msa1401140;Name=Msa1401140 |
| tig0022500 | mRNA | 20188 | 21135 | 20188 | ID=Msa1401140-mRNA-1;Parent=Msa1401140;Name=Msa1401140-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|315 |
| tig0022500 | exon | 20188 | 21135 | 20188 | ID=Msa1401140-mRNA-1:exon:18199;Parent=Msa1401140-mRNA-1 |
| tig0022500 | CDS | 20188 | 21135 | 20188 | ID=Msa1401140-mRNA-1:cds;Parent=Msa1401140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
