AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0810760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0810760 MtrunA17_Chr5g0427021 95.361 194 2 2 1 193 1 188 1.18e-132 369
Msa0810760 MtrunA17_Chr7g0231881 46.970 66 33 1 14 79 5 68 8.64e-14 65.1
Msa0810760 MtrunA17_Chr8g0334651 45.614 57 31 0 17 73 8 64 4.30e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0810760 AT1G02070.1 55.102 49 21 1 17 64 5 53 1.15e-11 60.1

Find 28 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTTCTGATGGTGATTT+TGG 0.180402 5_3:-39627846 None:intergenic
TTTGGTTTCTTCATTGGTTT+TGG 0.200009 5_3:-39627828 None:intergenic
GATGAAGAATTTGAAGAGTT+TGG 0.223033 5_3:-39627693 None:intergenic
ACCTGTGTTGAGATCGGATT+TGG 0.307776 5_3:-39627223 None:intergenic
CATTGAGAGGTGAGAGGGAG+AGG 0.322912 5_3:-39627076 None:intergenic
CTTATTCCTGAAAACTTGTT+TGG 0.327849 5_3:-39628012 None:intergenic
AAAAGTAGTAATAATAATAT+TGG 0.354978 5_3:+39627984 Msa0810760:CDS
CGAGTTGAAACTAAAATAGA+TGG 0.362041 5_3:-39627894 None:intergenic
CACATGCAACACTCTGTCTT+TGG 0.364915 5_3:-39627155 None:intergenic
TGTTGCATGTGTGGGGATCT+TGG 0.374832 5_3:+39627167 Msa0810760:CDS
AATCATCACAAACGGTGCTT+TGG 0.403206 5_3:+39627923 Msa0810760:CDS
ATTGAGAGGTGAGAGGGAGA+GGG 0.479847 5_3:-39627075 None:intergenic
CGCAAAAGAATCATCACAAA+CGG 0.503705 5_3:+39627915 Msa0810760:CDS
CAGAAGAAAGGAGTAATCAA+AGG 0.511163 5_3:+39627780 Msa0810760:CDS
TTAAATACCTGTGTTGAGAT+CGG 0.514874 5_3:-39627229 None:intergenic
GGAGATGTTGATGATGCTAT+TGG 0.539445 5_3:-39627873 None:intergenic
AAAGGAGTAATCAAAGGAAG+TGG 0.542816 5_3:+39627786 Msa0810760:CDS
TTGAAGATAGATGAAGAACT+AGG 0.558656 5_3:-39627036 None:intergenic
AATGAAAGCACAGAAGATGA+AGG 0.559578 5_3:+39627729 Msa0810760:CDS
AGTCTATATCTTGTTTAACT+CGG 0.560190 5_3:+39626959 Msa0810760:five_prime_UTR
CAGCAAAACATGACAAGCAA+AGG 0.581246 5_3:+39627110 Msa0810760:exon
AAGACAGAGTGTTGCATGTG+TGG 0.591608 5_3:+39627158 Msa0810760:CDS
GATAGATGAAGAACTAGGAG+AGG 0.593712 5_3:-39627031 None:intergenic
GTGCTTTGGAAGAGAAGTTG+AGG 0.598720 5_3:+39627937 Msa0810760:CDS
AGACAGAGTGTTGCATGTGT+GGG 0.608298 5_3:+39627159 Msa0810760:CDS
GCCAAATCCGATCTCAACAC+AGG 0.610494 5_3:+39627222 Msa0810760:CDS
GACAGAGTGTTGCATGTGTG+GGG 0.680628 5_3:+39627160 Msa0810760:CDS
TTGGTGCTTGAGTCAGAACG+AGG 0.698443 5_3:+39627665 Msa0810760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTAAAAAAAAGATTAA+GGG - chr5_3:39627275-39627294 None:intergenic 10.0%
!! TTTCATTAAAAAAAAGATTA+AGG - chr5_3:39627276-39627295 None:intergenic 10.0%
!! AAAAGTAGTAATAATAATAT+TGG + chr5_3:39627984-39628003 Msa0810760:CDS 10.0%
!! TTCATTAAAAAAAAGATTAA+GGG - chr5_3:39627275-39627294 None:intergenic 10.0%
!! TTTCATTAAAAAAAAGATTA+AGG - chr5_3:39627276-39627295 None:intergenic 10.0%
!! AAAAGTAGTAATAATAATAT+TGG + chr5_3:39627984-39628003 Msa0810760:CDS 10.0%
!!! TTTTGTTATTTAATTTGTTG+TGG + chr5_3:39627552-39627571 Msa0810760:intron 15.0%
!!! TTTTGTTATTTAATTTGTTG+TGG + chr5_3:39627552-39627571 Msa0810760:intron 15.0%
!!! TATTTTTGTAAGGAAATTGA+AGG + chr5_3:39627443-39627462 Msa0810760:intron 20.0%
!! TTATATATACATCTTTAGTG+AGG + chr5_3:39627479-39627498 Msa0810760:intron 20.0%
!!! AATTTTTTGGAACATGTAAT+TGG + chr5_3:39627646-39627665 Msa0810760:CDS 20.0%
!!! AAAAATTGTTTTCAGAAGAA+AGG + chr5_3:39627768-39627787 Msa0810760:CDS 20.0%
!!! TATTTTTGTAAGGAAATTGA+AGG + chr5_3:39627443-39627462 Msa0810760:intron 20.0%
!! TTATATATACATCTTTAGTG+AGG + chr5_3:39627479-39627498 Msa0810760:intron 20.0%
!!! AATTTTTTGGAACATGTAAT+TGG + chr5_3:39627646-39627665 Msa0810760:CDS 20.0%
!!! AAAAATTGTTTTCAGAAGAA+AGG + chr5_3:39627768-39627787 Msa0810760:CDS 20.0%
!! AGTCTATATCTTGTTTAACT+CGG + chr5_3:39626959-39626978 Msa0810760:five_prime_UTR 25.0%
!!! CTCTTTACCTTTTTTATTTG+TGG + chr5_3:39627001-39627020 Msa0810760:five_prime_UTR 25.0%
! CTAATGACCACAAATAAAAA+AGG - chr5_3:39627011-39627030 None:intergenic 25.0%
!!! TGTATCTATTTTTCATTGAG+AGG - chr5_3:39627092-39627111 None:intergenic 25.0%
!!! GAGTTACATATAGTTTTGAA+AGG + chr5_3:39627387-39627406 Msa0810760:intron 25.0%
!!! ATTTAATTTGTTGTGGTCTT+TGG + chr5_3:39627559-39627578 Msa0810760:intron 25.0%
!! TACCTACAAAATTTTCATGA+AGG - chr5_3:39627600-39627619 None:intergenic 25.0%
!!! TATCCAACAACAGAATTTTT+TGG + chr5_3:39627633-39627652 Msa0810760:CDS 25.0%
!!! TAGATGAAGTTTCAAGTTAA+CGG + chr5_3:39628054-39628073 Msa0810760:CDS 25.0%
!! AGTCTATATCTTGTTTAACT+CGG + chr5_3:39626959-39626978 Msa0810760:five_prime_UTR 25.0%
!!! CTCTTTACCTTTTTTATTTG+TGG + chr5_3:39627001-39627020 Msa0810760:five_prime_UTR 25.0%
! CTAATGACCACAAATAAAAA+AGG - chr5_3:39627011-39627030 None:intergenic 25.0%
!!! TGTATCTATTTTTCATTGAG+AGG - chr5_3:39627092-39627111 None:intergenic 25.0%
!!! GAGTTACATATAGTTTTGAA+AGG + chr5_3:39627387-39627406 Msa0810760:intron 25.0%
!!! ATTTAATTTGTTGTGGTCTT+TGG + chr5_3:39627559-39627578 Msa0810760:intron 25.0%
!! TACCTACAAAATTTTCATGA+AGG - chr5_3:39627600-39627619 None:intergenic 25.0%
!!! TATCCAACAACAGAATTTTT+TGG + chr5_3:39627633-39627652 Msa0810760:CDS 25.0%
TTGAAGATAGATGAAGAACT+AGG - chr5_3:39627039-39627058 None:intergenic 30.0%
! AGATTTTGCATAGAAAAAGC+TGG - chr5_3:39627202-39627221 None:intergenic 30.0%
TTAAATACCTGTGTTGAGAT+CGG - chr5_3:39627232-39627251 None:intergenic 30.0%
! CTCCTTCATGAAAATTTTGT+AGG + chr5_3:39627595-39627614 Msa0810760:intron 30.0%
GTTCCAAAAAATTCTGTTGT+TGG - chr5_3:39627639-39627658 None:intergenic 30.0%
GATGAAGAATTTGAAGAGTT+TGG - chr5_3:39627696-39627715 None:intergenic 30.0%
!!! TTTGGTTTCTTCATTGGTTT+TGG - chr5_3:39627831-39627850 None:intergenic 30.0%
! CGAGTTGAAACTAAAATAGA+TGG - chr5_3:39627897-39627916 None:intergenic 30.0%
! CTTATTCCTGAAAACTTGTT+TGG - chr5_3:39628015-39628034 None:intergenic 30.0%
!! CAAACAAGTTTTCAGGAATA+AGG + chr5_3:39628013-39628032 Msa0810760:CDS 30.0%
!! TTTTCAGGAATAAGGTAAGA+AGG + chr5_3:39628021-39628040 Msa0810760:CDS 30.0%
TTGAAGATAGATGAAGAACT+AGG - chr5_3:39627039-39627058 None:intergenic 30.0%
! AGATTTTGCATAGAAAAAGC+TGG - chr5_3:39627202-39627221 None:intergenic 30.0%
TTAAATACCTGTGTTGAGAT+CGG - chr5_3:39627232-39627251 None:intergenic 30.0%
! CTCCTTCATGAAAATTTTGT+AGG + chr5_3:39627595-39627614 Msa0810760:intron 30.0%
GTTCCAAAAAATTCTGTTGT+TGG - chr5_3:39627639-39627658 None:intergenic 30.0%
GATGAAGAATTTGAAGAGTT+TGG - chr5_3:39627696-39627715 None:intergenic 30.0%
!!! TTTGGTTTCTTCATTGGTTT+TGG - chr5_3:39627831-39627850 None:intergenic 30.0%
! CGAGTTGAAACTAAAATAGA+TGG - chr5_3:39627897-39627916 None:intergenic 30.0%
! CTTATTCCTGAAAACTTGTT+TGG - chr5_3:39628015-39628034 None:intergenic 30.0%
!! CAAACAAGTTTTCAGGAATA+AGG + chr5_3:39628013-39628032 Msa0810760:CDS 30.0%
!! TTTTCAGGAATAAGGTAAGA+AGG + chr5_3:39628021-39628040 Msa0810760:CDS 30.0%
!!! ACTCGGTTTAGTCTTTTTTG+TGG + chr5_3:39626976-39626995 Msa0810760:five_prime_UTR 35.0%
!!! CTCGGTTTAGTCTTTTTTGT+GGG + chr5_3:39626977-39626996 Msa0810760:five_prime_UTR 35.0%
! TTTTTCATTGAGAGGTGAGA+GGG - chr5_3:39627084-39627103 None:intergenic 35.0%
! ATTTTTCATTGAGAGGTGAG+AGG - chr5_3:39627085-39627104 None:intergenic 35.0%
! AATGAAAGCACAGAAGATGA+AGG + chr5_3:39627729-39627748 Msa0810760:CDS 35.0%
CAGAAGAAAGGAGTAATCAA+AGG + chr5_3:39627780-39627799 Msa0810760:CDS 35.0%
AAAGGAGTAATCAAAGGAAG+TGG + chr5_3:39627786-39627805 Msa0810760:CDS 35.0%
!!! GGTGATTTTGGTTTCTTCAT+TGG - chr5_3:39627837-39627856 None:intergenic 35.0%
!! TTTGCTTCTGATGGTGATTT+TGG - chr5_3:39627849-39627868 None:intergenic 35.0%
!! ATTGGTGATTTTGCTTCTGA+TGG - chr5_3:39627858-39627877 None:intergenic 35.0%
CGCAAAAGAATCATCACAAA+CGG + chr5_3:39627915-39627934 Msa0810760:CDS 35.0%
!!! ACTCGGTTTAGTCTTTTTTG+TGG + chr5_3:39626976-39626995 Msa0810760:five_prime_UTR 35.0%
!!! CTCGGTTTAGTCTTTTTTGT+GGG + chr5_3:39626977-39626996 Msa0810760:five_prime_UTR 35.0%
! TTTTTCATTGAGAGGTGAGA+GGG - chr5_3:39627084-39627103 None:intergenic 35.0%
! ATTTTTCATTGAGAGGTGAG+AGG - chr5_3:39627085-39627104 None:intergenic 35.0%
! AATGAAAGCACAGAAGATGA+AGG + chr5_3:39627729-39627748 Msa0810760:CDS 35.0%
CAGAAGAAAGGAGTAATCAA+AGG + chr5_3:39627780-39627799 Msa0810760:CDS 35.0%
AAAGGAGTAATCAAAGGAAG+TGG + chr5_3:39627786-39627805 Msa0810760:CDS 35.0%
!!! GGTGATTTTGGTTTCTTCAT+TGG - chr5_3:39627837-39627856 None:intergenic 35.0%
!! TTTGCTTCTGATGGTGATTT+TGG - chr5_3:39627849-39627868 None:intergenic 35.0%
!! ATTGGTGATTTTGCTTCTGA+TGG - chr5_3:39627858-39627877 None:intergenic 35.0%
CGCAAAAGAATCATCACAAA+CGG + chr5_3:39627915-39627934 Msa0810760:CDS 35.0%
GATAGATGAAGAACTAGGAG+AGG - chr5_3:39627034-39627053 None:intergenic 40.0%
CAGCAAAACATGACAAGCAA+AGG + chr5_3:39627110-39627129 Msa0810760:exon 40.0%
!! GGAGATGTTGATGATGCTAT+TGG - chr5_3:39627876-39627895 None:intergenic 40.0%
AATCATCACAAACGGTGCTT+TGG + chr5_3:39627923-39627942 Msa0810760:CDS 40.0%
! GTGTGACCAAACAAGTTTTC+AGG + chr5_3:39628006-39628025 Msa0810760:CDS 40.0%
GATAGATGAAGAACTAGGAG+AGG - chr5_3:39627034-39627053 None:intergenic 40.0%
CAGCAAAACATGACAAGCAA+AGG + chr5_3:39627110-39627129 Msa0810760:exon 40.0%
!! GGAGATGTTGATGATGCTAT+TGG - chr5_3:39627876-39627895 None:intergenic 40.0%
AATCATCACAAACGGTGCTT+TGG + chr5_3:39627923-39627942 Msa0810760:CDS 40.0%
! GTGTGACCAAACAAGTTTTC+AGG + chr5_3:39628006-39628025 Msa0810760:CDS 40.0%
CACATGCAACACTCTGTCTT+TGG - chr5_3:39627158-39627177 None:intergenic 45.0%
!! AAGACAGAGTGTTGCATGTG+TGG + chr5_3:39627158-39627177 Msa0810760:CDS 45.0%
!! AGACAGAGTGTTGCATGTGT+GGG + chr5_3:39627159-39627178 Msa0810760:CDS 45.0%
ACCTGTGTTGAGATCGGATT+TGG - chr5_3:39627226-39627245 None:intergenic 45.0%
!! GTGCTTTGGAAGAGAAGTTG+AGG + chr5_3:39627937-39627956 Msa0810760:CDS 45.0%
CACATGCAACACTCTGTCTT+TGG - chr5_3:39627158-39627177 None:intergenic 45.0%
!! AAGACAGAGTGTTGCATGTG+TGG + chr5_3:39627158-39627177 Msa0810760:CDS 45.0%
!! AGACAGAGTGTTGCATGTGT+GGG + chr5_3:39627159-39627178 Msa0810760:CDS 45.0%
ACCTGTGTTGAGATCGGATT+TGG - chr5_3:39627226-39627245 None:intergenic 45.0%
!! GTGCTTTGGAAGAGAAGTTG+AGG + chr5_3:39627937-39627956 Msa0810760:CDS 45.0%
!!! ATAAATTTTATATTTTTGTA+AGG + chr5_3:39627433-39627452 Msa0810760:intron 5.0%
!!! ATAAATTTTATATTTTTGTA+AGG + chr5_3:39627433-39627452 Msa0810760:intron 5.0%
ATTGAGAGGTGAGAGGGAGA+GGG - chr5_3:39627078-39627097 None:intergenic 50.0%
!! GACAGAGTGTTGCATGTGTG+GGG + chr5_3:39627160-39627179 Msa0810760:CDS 50.0%
TGTTGCATGTGTGGGGATCT+TGG + chr5_3:39627167-39627186 Msa0810760:CDS 50.0%
GCCAAATCCGATCTCAACAC+AGG + chr5_3:39627222-39627241 Msa0810760:CDS 50.0%
! TTGGTGCTTGAGTCAGAACG+AGG + chr5_3:39627665-39627684 Msa0810760:CDS 50.0%
ATTGAGAGGTGAGAGGGAGA+GGG - chr5_3:39627078-39627097 None:intergenic 50.0%
!! GACAGAGTGTTGCATGTGTG+GGG + chr5_3:39627160-39627179 Msa0810760:CDS 50.0%
TGTTGCATGTGTGGGGATCT+TGG + chr5_3:39627167-39627186 Msa0810760:CDS 50.0%
GCCAAATCCGATCTCAACAC+AGG + chr5_3:39627222-39627241 Msa0810760:CDS 50.0%
! TTGGTGCTTGAGTCAGAACG+AGG + chr5_3:39627665-39627684 Msa0810760:CDS 50.0%
CATTGAGAGGTGAGAGGGAG+AGG - chr5_3:39627079-39627098 None:intergenic 55.0%
CATTGAGAGGTGAGAGGGAG+AGG - chr5_3:39627079-39627098 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 39626947 39628073 39626947 ID=Msa0810760;Name=Msa0810760
chr5_3 mRNA 39626947 39628073 39626947 ID=Msa0810760-mRNA-1;Parent=Msa0810760;Name=Msa0810760-mRNA-1;_AED=0.03;_eAED=0.03;_QI=172|1|0.5|1|1|1|2|0|193
chr5_3 exon 39626947 39627243 39626947 ID=Msa0810760-mRNA-1:exon:11998;Parent=Msa0810760-mRNA-1
chr5_3 exon 39627617 39628073 39627617 ID=Msa0810760-mRNA-1:exon:11999;Parent=Msa0810760-mRNA-1
chr5_3 five_prime_UTR 39626947 39627118 39626947 ID=Msa0810760-mRNA-1:five_prime_utr;Parent=Msa0810760-mRNA-1
chr5_3 CDS 39627119 39627243 39627119 ID=Msa0810760-mRNA-1:cds;Parent=Msa0810760-mRNA-1
chr5_3 CDS 39627617 39628073 39627617 ID=Msa0810760-mRNA-1:cds;Parent=Msa0810760-mRNA-1
Gene Sequence

>Msa0810760

ATGACAAGCAAAGGAACAAACTCTGCAGAACAACTACCAAAGACAGAGTGTTGCATGTGTGGGGATCTTGGTTTCTCTGACCAGCTTTTTCTATGCAAAATCTGCCAAATCCGATCTCAACACAGGTATTGTAGTAACATATATCCAACAACAGAATTTTTTGGAACATGTAATTGGTGCTTGAGTCAGAACGAGGATTCACCAAACTCTTCAAATTCTTCATCTTCTTACAAGAAAAATGAAAGCACAGAAGATGAAGGAAAGAACAAACAAATAAAAAATTGTTTTCAGAAGAAAGGAGTAATCAAAGGAAGTGGTGTTTATCGTCTTCAACTTCCAAAACCAATGAAGAAACCAAAATCACCATCAGAAGCAAAATCACCAATAGCATCATCAACATCTCCATCTATTTTAGTTTCAACTCGCAAAAGAATCATCACAAACGGTGCTTTGGAAGAGAAGTTGAGGAGAACAAGATCAGAAAACATTATAAAAAGTAGTAATAATAATATTGGTGTGACCAAACAAGTTTTCAGGAATAAGGTAAGAAGGTATAAGCTTTTAGATGAAGTTTCAAGTTAA

Protein sequence

>Msa0810760

MTSKGTNSAEQLPKTECCMCGDLGFSDQLFLCKICQIRSQHRYCSNIYPTTEFFGTCNWCLSQNEDSPNSSNSSSSYKKNESTEDEGKNKQIKNCFQKKGVIKGSGVYRLQLPKPMKKPKSPSEAKSPIASSTSPSILVSTRKRIITNGALEEKLRRTRSENIIKSSNNNIGVTKQVFRNKVRRYKLLDEVSS*