AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216690 MtrunA17_Chr4g0066371 94.987 399 20 0 1 399 1 399 0.0 790
Msa1216690 MtrunA17_Chr2g0286951 68.983 403 119 4 1 399 1 401 0.0 551
Msa1216690 MtrunA17_Chr6g0486661 60.542 332 125 3 15 343 56 384 1.09e-133 409
Msa1216690 MtrunA17_Chr7g0243571 27.707 314 163 6 46 323 60 345 3.14e-31 123
Msa1216690 MtrunA17_Chr1g0192421 29.391 279 156 6 68 323 71 331 1.68e-30 120
Msa1216690 MtrunA17_Chr7g0221691 30.976 297 143 6 68 322 111 387 2.71e-30 121
Msa1216690 MtrunA17_Chr4g0004971 28.070 285 160 6 68 325 60 326 2.75e-30 119
Msa1216690 MtrunA17_Chr2g0295401 28.972 321 171 9 44 336 58 349 3.35e-29 116
Msa1216690 MtrunA17_Chr1g0150451 32.090 268 139 10 84 350 106 331 4.67e-29 115
Msa1216690 MtrunA17_Chr3g0136711 30.671 313 164 13 44 350 28 293 4.89e-29 114
Msa1216690 MtrunA17_Chr7g0249421 26.374 364 190 8 68 371 78 423 1.05e-28 117
Msa1216690 MtrunA17_Chr7g0272061 26.885 305 171 6 68 338 73 359 7.81e-28 113
Msa1216690 MtrunA17_Chr4g0067711 28.114 281 159 6 68 324 73 334 3.81e-27 110
Msa1216690 MtrunA17_Chr2g0324061 27.244 312 171 7 68 342 73 365 3.86e-27 112
Msa1216690 MtrunA17_Chr1g0151781 28.125 256 143 6 68 301 51 287 8.45e-27 109
Msa1216690 MtrunA17_Chr8g0342431 26.994 326 160 9 68 338 68 370 9.74e-27 110
Msa1216690 MtrunA17_Chr3g0105351 28.472 288 148 12 66 341 74 315 2.33e-26 107
Msa1216690 MtrunA17_Chr3g0105371 28.571 287 149 12 66 341 98 339 7.79e-26 107
Msa1216690 MtrunA17_Chr4g0022171 26.332 319 178 7 68 348 79 378 1.61e-25 107
Msa1216690 MtrunA17_Chr1g0212961 30.682 264 139 11 76 339 117 336 1.78e-25 105
Msa1216690 MtrunA17_Chr5g0430481 29.181 281 149 12 69 341 137 375 2.29e-25 106
Msa1216690 MtrunA17_Chr2g0317021 28.623 276 152 10 69 341 100 333 1.60e-24 103
Msa1216690 MtrunA17_Chr3g0126511 29.304 273 152 9 69 340 108 340 2.56e-24 103
Msa1216690 MtrunA17_Chr4g0065141 31.599 269 128 12 84 338 122 348 1.03e-23 101
Msa1216690 MtrunA17_Chr5g0407161 28.626 262 136 10 86 338 124 343 3.15e-22 97.4
Msa1216690 MtrunA17_Chr1g0150781 25.610 328 163 12 49 341 263 544 1.48e-20 93.6
Msa1216690 MtrunA17_Chr2g0293811 27.957 279 140 9 86 345 100 336 1.76e-20 92.0
Msa1216690 MtrunA17_Chr5g0426141 25.298 336 194 13 7 341 3 282 2.83e-20 90.1
Msa1216690 MtrunA17_Chr4g0051661 30.233 258 136 13 83 338 180 395 3.82e-19 88.6
Msa1216690 MtrunA17_Chr4g0070871 28.270 237 119 8 82 304 249 448 5.69e-19 88.6
Msa1216690 MtrunA17_Chr8g0360351 27.451 255 144 9 86 339 66 280 1.13e-18 85.5
Msa1216690 MtrunA17_Chr1g0194191 28.369 282 141 11 86 348 118 357 1.35e-18 86.7
Msa1216690 MtrunA17_Chr3g0138281 28.679 265 135 10 86 338 121 343 4.05e-18 85.5
Msa1216690 MtrunA17_Chr3g0112291 28.340 247 125 10 69 298 106 317 5.81e-18 84.7
Msa1216690 MtrunA17_Chr8g0389921 26.007 273 154 8 84 341 196 435 1.44e-17 84.3
Msa1216690 MtrunA17_Chr5g0396731 28.761 226 116 6 86 301 107 297 3.49e-16 79.3
Msa1216690 MtrunA17_Chr3g0144111 27.481 262 118 10 86 338 27 225 8.33e-16 77.0
Msa1216690 MtrunA17_Chr1g0171571 27.972 286 158 11 69 344 401 648 1.06e-14 75.9
Msa1216690 MtrunA17_Chr1g0185141 27.778 252 104 9 147 349 225 447 5.07e-14 73.9
Msa1216690 MtrunA17_Chr5g0435721 29.046 241 112 13 85 304 222 424 2.28e-13 71.6
Msa1216690 MtrunA17_Chr7g0225501 27.519 258 136 12 59 296 54 280 4.60e-12 67.4
Msa1216690 MtrunA17_Chr1g0209451 27.703 296 152 14 66 340 43 297 5.10e-12 67.0
Msa1216690 MtrunA17_Chr7g0271971 27.946 297 154 14 64 340 41 297 6.48e-12 66.6
Msa1216690 MtrunA17_Chr1g0192471 28.571 308 148 17 59 340 36 297 1.09e-11 66.2
Msa1216690 MtrunA17_Chr1g0175381 26.190 252 140 11 86 313 79 308 4.81e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216690 AT3G06270.1 68.012 347 109 1 1 345 1 347 4.19e-174 489
Msa1216690 AT2G20050.1 63.323 319 113 1 40 354 97 415 1.88e-138 422
Msa1216690 AT2G20050.2 63.323 319 113 1 40 354 97 415 2.09e-138 422
Msa1216690 AT5G27930.1 27.609 297 171 6 68 338 74 352 2.70e-31 123
Msa1216690 AT5G27930.2 27.609 297 171 6 68 338 74 352 2.70e-31 123
Msa1216690 AT5G26010.1 32.479 234 128 5 80 301 67 282 3.37e-31 122
Msa1216690 AT5G10740.1 32.155 283 149 10 60 339 36 278 9.09e-30 118
Msa1216690 AT5G10740.2 32.155 283 149 10 60 339 36 278 9.09e-30 118
Msa1216690 AT5G36250.1 27.327 333 174 7 68 350 80 394 9.30e-30 120
Msa1216690 AT3G05640.2 30.182 275 156 6 68 324 75 331 1.64e-29 117
Msa1216690 AT3G05640.1 30.182 275 156 6 68 324 75 331 1.64e-29 117
Msa1216690 AT3G05640.3 30.182 275 156 6 68 324 75 331 1.64e-29 117
Msa1216690 AT4G31750.2 31.907 257 134 9 84 339 62 278 2.96e-29 115
Msa1216690 AT4G31750.1 31.907 257 134 9 84 339 62 278 4.33e-29 115
Msa1216690 AT4G31750.3 31.907 257 134 9 84 339 62 278 4.33e-29 115
Msa1216690 AT4G03415.1 27.187 320 175 8 68 348 80 380 1.13e-28 117
Msa1216690 AT4G03415.3 27.187 320 175 8 68 348 80 380 1.13e-28 117
Msa1216690 AT4G03415.2 27.187 320 175 8 68 348 80 380 1.13e-28 117
Msa1216690 AT4G32950.1 31.298 262 152 8 88 340 74 316 2.04e-28 114
Msa1216690 AT5G24940.1 32.443 262 126 10 84 339 62 278 8.78e-28 114
Msa1216690 AT5G01700.1 26.403 303 168 6 68 340 11 288 2.14e-27 111
Msa1216690 AT5G01700.2 26.403 303 168 6 68 340 60 337 3.21e-27 112
Msa1216690 AT1G03590.1 27.974 311 169 8 68 342 73 364 7.30e-27 112
Msa1216690 AT5G36250.2 26.225 347 174 8 68 350 80 408 1.65e-26 110
Msa1216690 AT5G27930.3 29.457 258 138 6 68 299 74 313 2.08e-26 108
Msa1216690 AT1G78200.1 29.492 295 164 13 49 341 31 283 7.52e-26 106
Msa1216690 AT1G78200.3 29.492 295 164 13 49 341 31 283 7.52e-26 106
Msa1216690 AT1G78200.2 29.492 295 164 13 49 341 31 283 7.52e-26 106
Msa1216690 AT1G16220.1 26.048 334 181 7 68 356 77 389 1.89e-25 108
Msa1216690 AT3G16800.2 27.931 290 172 6 68 338 75 346 2.51e-25 105
Msa1216690 AT3G16800.1 27.931 290 172 6 68 338 75 346 2.51e-25 105
Msa1216690 AT3G16800.3 27.931 290 172 6 68 338 75 346 2.51e-25 105
Msa1216690 AT1G78200.5 30.597 268 145 12 76 341 10 238 1.48e-24 101
Msa1216690 AT1G78200.4 30.597 268 145 12 76 341 10 238 1.48e-24 101
Msa1216690 AT3G02750.2 29.091 275 164 7 110 379 177 425 1.50e-24 105
Msa1216690 AT3G02750.1 29.091 275 164 7 110 379 177 425 1.50e-24 105
Msa1216690 AT5G36250.3 28.519 270 127 6 68 289 80 331 3.15e-24 102
Msa1216690 AT5G53140.1 27.007 274 159 9 69 341 115 348 5.46e-24 103
Msa1216690 AT5G53140.2 27.007 274 159 9 69 341 115 348 5.46e-24 103
Msa1216690 AT3G51470.2 29.252 294 153 13 58 338 30 281 7.36e-24 101
Msa1216690 AT3G51470.1 29.252 294 153 13 58 338 74 325 1.51e-23 101
Msa1216690 AT4G32950.2 34.450 209 111 7 88 289 74 263 3.53e-23 98.6
Msa1216690 AT3G16800.4 27.881 269 157 6 89 338 1 251 5.12e-23 97.8
Msa1216690 AT3G16800.6 27.881 269 157 6 89 338 1 251 5.12e-23 97.8
Msa1216690 AT1G07160.1 28.470 281 152 9 69 340 136 376 5.53e-23 99.8
Msa1216690 AT1G79630.2 30.000 200 119 4 142 338 146 327 1.87e-22 99.0
Msa1216690 AT1G79630.7 30.000 200 119 4 142 338 146 327 1.87e-22 99.0
Msa1216690 AT1G79630.1 30.000 200 119 4 142 338 216 397 2.04e-22 99.4
Msa1216690 AT1G79630.8 30.000 200 119 4 142 338 212 393 2.26e-22 99.4
Msa1216690 AT1G79630.6 30.000 200 119 4 142 338 106 287 2.29e-22 98.2
Msa1216690 AT1G79630.3 30.000 200 119 4 142 338 106 287 2.29e-22 98.2
Msa1216690 AT1G79630.4 30.000 200 119 4 142 338 106 287 2.29e-22 98.2
Msa1216690 AT1G79630.5 30.000 200 119 4 142 338 106 287 2.29e-22 98.2
Msa1216690 AT1G43900.1 28.467 274 154 9 69 341 137 369 6.14e-22 96.7
Msa1216690 AT5G57050.2 26.471 306 152 10 81 350 113 381 3.03e-19 89.0
Msa1216690 AT5G57050.1 26.471 306 152 10 81 350 153 421 3.80e-19 89.0
Msa1216690 AT1G34750.2 26.966 267 153 11 76 341 57 282 4.07e-19 87.0
Msa1216690 AT1G34750.1 26.966 267 153 11 76 341 57 282 4.07e-19 87.0
Msa1216690 AT1G34750.3 26.966 267 153 11 76 341 57 282 4.07e-19 87.0
Msa1216690 AT2G40180.1 30.855 269 130 13 86 341 161 386 4.21e-19 88.6
Msa1216690 AT3G02750.3 28.188 298 172 10 110 379 177 460 5.81e-19 89.4
Msa1216690 AT1G48040.1 26.909 275 136 9 86 338 116 347 7.91e-19 87.8
Msa1216690 AT2G25620.1 27.941 272 138 10 86 341 128 357 1.35e-18 87.4
Msa1216690 AT2G25620.2 27.941 272 138 10 86 341 128 357 1.35e-18 87.4
Msa1216690 AT2G20630.1 26.538 260 150 9 83 341 60 279 1.99e-18 85.1
Msa1216690 AT3G15260.2 27.132 258 145 9 86 341 73 289 2.67e-18 85.1
Msa1216690 AT3G15260.1 27.132 258 145 9 86 341 73 289 2.67e-18 85.1
Msa1216690 AT2G30020.1 27.972 286 148 11 69 341 153 393 2.93e-18 86.3
Msa1216690 AT2G33700.1 27.099 262 140 9 86 338 123 342 5.92e-18 85.1
Msa1216690 AT1G72770.4 26.557 305 139 11 85 342 237 503 9.04e-18 85.5
Msa1216690 AT1G72770.1 26.557 305 139 11 85 342 237 503 9.04e-18 85.5
Msa1216690 AT1G72770.3 26.557 305 139 11 85 342 237 503 9.04e-18 85.5
Msa1216690 AT1G22280.1 24.068 295 179 11 48 341 31 281 9.33e-18 83.2
Msa1216690 AT4G28400.3 27.059 255 145 9 86 339 45 259 1.24e-17 82.4
Msa1216690 AT2G20630.2 26.562 256 147 9 83 337 60 275 1.38e-17 82.8
Msa1216690 AT4G28400.1 27.059 255 145 9 86 339 67 281 1.64e-17 82.4
Msa1216690 AT5G51760.2 29.412 255 118 8 75 302 103 322 6.08e-17 82.4
Msa1216690 AT5G51760.1 29.412 255 118 8 75 302 133 352 6.40e-17 82.4
Msa1216690 AT3G17250.1 26.619 278 139 10 84 338 156 391 8.01e-17 82.0
Msa1216690 AT1G09160.2 31.788 302 146 19 59 340 36 297 6.49e-16 79.3
Msa1216690 AT1G09160.1 31.788 302 146 19 59 340 36 297 6.49e-16 79.3
Msa1216690 AT1G22280.3 23.920 301 178 12 48 341 31 287 7.51e-16 77.8
Msa1216690 AT2G34740.1 25.000 272 161 10 69 338 102 332 7.56e-16 78.6
Msa1216690 AT2G29380.1 25.952 289 144 13 85 341 111 361 8.53e-15 75.5
Msa1216690 AT1G68410.4 30.743 296 143 17 66 340 48 302 2.02e-13 72.0
Msa1216690 AT1G68410.3 30.743 296 143 17 66 340 48 302 2.02e-13 72.0
Msa1216690 AT1G68410.1 30.743 296 143 17 66 340 48 302 2.02e-13 72.0
Msa1216690 AT1G68410.2 30.743 296 143 17 66 340 48 302 2.02e-13 72.0
Msa1216690 AT3G11410.1 27.347 245 117 11 83 302 134 342 4.73e-13 70.5
Msa1216690 AT2G34740.2 26.222 225 128 9 69 291 116 304 1.01e-12 68.9
Msa1216690 AT2G34740.3 26.222 225 128 9 69 291 98 286 1.11e-12 68.6
Msa1216690 AT5G57050.3 33.333 144 73 6 81 209 153 288 1.45e-11 65.5
Msa1216690 AT5G02760.1 25.397 315 145 14 81 340 65 344 1.57e-11 65.9
Msa1216690 AT1G72770.2 31.373 153 71 6 85 209 237 383 2.94e-11 65.1
Msa1216690 AT1G72770.5 31.373 153 71 6 85 209 237 383 2.94e-11 65.1
Msa1216690 AT1G47380.1 26.174 298 172 13 66 349 39 302 6.53e-11 64.3
Msa1216690 AT1G34750.4 26.728 217 122 10 76 291 57 237 9.18e-11 62.4

Find 145 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTAGAAACTGTTTGATTT+TGG 0.169277 8_1:-78603134 None:intergenic
TCTCCAGGTGAGATTCTTTA+TGG 0.170664 8_1:-78606630 None:intergenic
TTTACCGGTCACTATATATT+TGG 0.177630 8_1:+78606440 Msa1216690:three_prime_UTR
TTGACTCTTCCTTCTTCATT+AGG 0.181058 8_1:+78602964 Msa1216690:five_prime_UTR
ATTATCATTGTCCAGATTAA+AGG 0.182707 8_1:+78605499 Msa1216690:CDS
CAAGTTGACGTGAAGAATTT+TGG 0.184990 8_1:-78602937 None:intergenic
AATTTCAAAAGGATCAAATT+TGG 0.225220 8_1:+78603332 Msa1216690:five_prime_UTR
TTACCCCAGTGTTGGATATT+TGG 0.226477 8_1:-78604139 None:intergenic
ACAGGATCCTTCACCAATTC+AGG 0.227180 8_1:-78603827 None:intergenic
TGACTCTTCCTTCTTCATTA+GGG 0.259340 8_1:+78602965 Msa1216690:five_prime_UTR
CCTCTGTTTCTCTTCTTTGA+AGG 0.266047 8_1:+78603002 Msa1216690:five_prime_UTR
CTGCATTTACAAGGAGTTTA+GGG 0.266150 8_1:+78604224 Msa1216690:CDS
TTGGGGTGACTTTGAGAAAA+AGG 0.273728 8_1:-78603115 None:intergenic
AGCTTACAATTCTGCGTTCT+CGG 0.277270 8_1:+78603853 Msa1216690:CDS
CATTGCTCCTGTACATTTAC+CGG 0.278853 8_1:+78606425 Msa1216690:three_prime_UTR
AACCCGAATATCCATTTCTT+TGG 0.294164 8_1:+78603722 Msa1216690:CDS
ACTGCATTTACAAGGAGTTT+AGG 0.296176 8_1:+78604223 Msa1216690:CDS
TTCCCAAATGGGATGTTTCC+TGG 0.311843 8_1:+78604199 Msa1216690:CDS
TCCTTCACACAACTTTGTTT+TGG 0.323600 8_1:+78603607 Msa1216690:CDS
ACACCAAAGAAATGGATATT+CGG 0.329115 8_1:-78603725 None:intergenic
GAAAATGCAATGAAGGTTCT+AGG 0.330257 8_1:-78603072 None:intergenic
TATTAGAGAGTCCTTTAATC+TGG 0.331443 8_1:-78605510 None:intergenic
TGATCAAGTGGAAGGGATTA+AGG 0.333034 8_1:+78604114 Msa1216690:CDS
CACCAACATTAGCAACATAA+AGG 0.344783 8_1:-78603961 None:intergenic
TTTGATGGGCATGGTCAATT+TGG 0.349419 8_1:+78603749 Msa1216690:CDS
TTCTTTATTGTTGCTAGTGA+TGG 0.360065 8_1:+78604322 Msa1216690:CDS
AAACTTTGTTAGAGATAGTT+TGG 0.363044 8_1:+78603784 Msa1216690:CDS
CACCAAAGAAATGGATATTC+GGG 0.365530 8_1:-78603724 None:intergenic
GAGTCAAATTTGAGCTCAAT+AGG 0.367013 8_1:-78603196 None:intergenic
GTTCCAGGAAACATCCCATT+TGG 0.370982 8_1:-78604202 None:intergenic
CTCCTTGTAAATGCAGTTCC+AGG 0.374803 8_1:-78604217 None:intergenic
ACATTGTAGTCAAGCACATC+TGG 0.378787 8_1:+78606026 Msa1216690:intron
GAGAGCTGTGATAGCTGTTA+AGG 0.383368 8_1:+78603988 Msa1216690:CDS
AAGCCAAACTGTTGTTGATA+TGG 0.388203 8_1:+78604363 Msa1216690:CDS
TGAAACATGGGTTGTGTTCA+TGG 0.388527 8_1:+78603476 Msa1216690:exon
ACAATAAAGAAGAGATGATT+AGG 0.391372 8_1:-78604310 None:intergenic
TTGTTTAGCCATACCCTCTC+TGG 0.391835 8_1:+78606586 Msa1216690:three_prime_UTR
TTTGATAGCAACAAACAAAT+GGG 0.402553 8_1:-78603250 None:intergenic
ATTTGATAGCAACAAACAAA+TGG 0.408534 8_1:-78603251 None:intergenic
AAGGAGTTTAGGGGATAGAT+TGG 0.411034 8_1:+78604234 Msa1216690:CDS
GTTATCTAACGACCCTGAAT+TGG 0.416488 8_1:+78603814 Msa1216690:CDS
GAGCTTGTATGTTCTTCCTA+TGG 0.417871 8_1:-78603559 None:intergenic
CGAGATTGTGGTTCCCAAAT+GGG 0.427237 8_1:+78604188 Msa1216690:CDS
TGCAAAAGACTCAGAAAAGT+TGG 0.428627 8_1:-78606404 None:intergenic
TACGAGAGAGTGAAACTTTG+TGG 0.431653 8_1:+78604073 Msa1216690:CDS
CCGAGATTGTGGTTCCCAAA+TGG 0.432081 8_1:+78604187 Msa1216690:CDS
CCTCTGATCAGACACCATTT+CGG 0.433454 8_1:+78604041 Msa1216690:CDS
CAGACAAGAAAATGCAATGA+AGG 0.437748 8_1:-78603079 None:intergenic
CTGAAGAGTCATATAAACTA+TGG 0.438665 8_1:+78605443 Msa1216690:CDS
GCTATTACAGTGCTTGTTGT+TGG 0.438993 8_1:+78603932 Msa1216690:CDS
TCCTTGTAGTTTCTCCTAAG+TGG 0.444679 8_1:+78606367 Msa1216690:three_prime_UTR
TTCCAGGAAACATCCCATTT+GGG 0.447799 8_1:-78604201 None:intergenic
TCAACTCATCTTTGACTGGC+AGG 0.448632 8_1:-78606174 None:intergenic
ATACCATATCAACAACAGTT+TGG 0.450437 8_1:-78604366 None:intergenic
ACTATATATTTGGTTGTTCA+AGG 0.453908 8_1:+78606450 Msa1216690:three_prime_UTR
GGGTATGAGGTTCATTGTCT+TGG 0.470077 8_1:+78606478 Msa1216690:three_prime_UTR
AACTGGGTCCTATTGTCATT+CGG 0.471259 8_1:+78606741 Msa1216690:three_prime_UTR
GATAGATTGGCGGAGACAAT+TGG 0.474209 8_1:+78604247 Msa1216690:CDS
CTGGGGTAACGAAGAGAGTT+GGG 0.475410 8_1:+78604153 Msa1216690:CDS
ATGTTGCTGTGTGAAATCTT+TGG 0.476177 8_1:+78603502 Msa1216690:CDS
ACCAACATTAGCAACATAAA+GGG 0.477778 8_1:-78603960 None:intergenic
GAGTGAAACTTTGTGGAGCA+AGG 0.485633 8_1:+78604080 Msa1216690:CDS
GTTCAAGGCAGCCGGGTATG+AGG 0.489981 8_1:+78606465 Msa1216690:three_prime_UTR
ATTTGGTTGTTCAAGGCAGC+CGG 0.490140 8_1:+78606457 Msa1216690:three_prime_UTR
AGTAGTACCTTTCGTGGGGT+GGG 0.490336 8_1:-78603035 None:intergenic
TCGTATTCATCTCTCCGAAA+TGG 0.490543 8_1:-78604055 None:intergenic
ACATGATACCCAATTTCAAA+AGG 0.491484 8_1:+78603321 Msa1216690:five_prime_UTR
TTCCTGGAACTGCATTTACA+AGG 0.492066 8_1:+78604215 Msa1216690:CDS
AATGAGAGAGACAGGAGAAG+TGG 0.495363 8_1:-78602914 None:intergenic
TGCATTAGGACGGAAATTCA+AGG 0.496046 8_1:+78603698 Msa1216690:CDS
AGAGCTGTGATAGCTGTTAA+GGG 0.499710 8_1:+78603989 Msa1216690:CDS
GCAGAATTGTAAGCTCGAAC+AGG 0.504003 8_1:-78603845 None:intergenic
GATGGCGGCTCTAGAAAACT+TGG 0.504312 8_1:+78603524 Msa1216690:CDS
ACACAACCCATGTTTCAAAC+AGG 0.505574 8_1:-78603470 None:intergenic
AGCTGAACTATTGACACCTC+TGG 0.506076 8_1:-78604283 None:intergenic
CAGAAAGTAGAAATTAGAGT+TGG 0.507926 8_1:+78606521 Msa1216690:three_prime_UTR
ACCCTTTATGTTGCTAATGT+TGG 0.524420 8_1:+78603959 Msa1216690:CDS
TTATTCAACTCATCTTTGAC+TGG 0.527302 8_1:-78606178 None:intergenic
CATCTCAGAAGTTCCTGTCC+TGG 0.530672 8_1:-78606090 None:intergenic
TTGAGTGTTGATCAAGTGGA+AGG 0.530984 8_1:+78604106 Msa1216690:CDS
AAGTAGTACCTTTCGTGGGG+TGG 0.532029 8_1:-78603036 None:intergenic
ACTGGGGTAACGAAGAGAGT+TGG 0.535227 8_1:+78604152 Msa1216690:CDS
GTAACCTCGCAGTGTGAGAA+AGG 0.536584 8_1:-78603636 None:intergenic
GTAGTACCTTTCGTGGGGTG+GGG 0.541925 8_1:-78603034 None:intergenic
TGTGATAGCTGTTAAGGGTG+GGG 0.542620 8_1:+78603994 Msa1216690:CDS
AAGGAAAACCCTAATGAAGA+AGG 0.543390 8_1:-78602973 None:intergenic
CCATTTGGGAACCACAATCT+CGG 0.544156 8_1:-78604187 None:intergenic
CTGTGATAGCTGTTAAGGGT+GGG 0.544181 8_1:+78603993 Msa1216690:CDS
TTTGGTTGTTCAAGGCAGCC+GGG 0.547435 8_1:+78606458 Msa1216690:three_prime_UTR
AGTGAAACTTTGTGGAGCAA+GGG 0.549706 8_1:+78604081 Msa1216690:CDS
ATAGTGACCGGTAAATGTAC+AGG 0.552466 8_1:-78606432 None:intergenic
AGGATCCAAATATCCAACAC+TGG 0.555282 8_1:+78604134 Msa1216690:CDS
TGAGTGTTGATCAAGTGGAA+GGG 0.556319 8_1:+78604107 Msa1216690:CDS
GAGTTTAGGGGATAGATTGG+CGG 0.561902 8_1:+78604237 Msa1216690:CDS
TCTCTTCGTTACCCCAGTGT+TGG 0.563931 8_1:-78604147 None:intergenic
ATATCACCTGTTTGAAACAT+GGG 0.564125 8_1:+78603464 Msa1216690:exon
GGATCCAAATATCCAACACT+GGG 0.565827 8_1:+78604135 Msa1216690:CDS
TCTTTACAGGGAACAAAGAG+AGG 0.567828 8_1:+78606320 Msa1216690:intron
GATATCACCTGTTTGAAACA+TGG 0.568845 8_1:+78603463 Msa1216690:exon
CTTTACAGGGAACAAAGAGA+GGG 0.574860 8_1:+78606321 Msa1216690:intron
ACTGCACCCCACCCCACGAA+AGG 0.577373 8_1:+78603028 Msa1216690:five_prime_UTR
GATACAATAAAACATTCTCC+AGG 0.580533 8_1:-78606645 None:intergenic
TTGGTTATCATCATAGCCAT+AGG 0.581172 8_1:+78603543 Msa1216690:CDS
TAATTTCTACTTTCTGAACA+AGG 0.582956 8_1:-78606514 None:intergenic
GGGTGGGGATCGGATTATTG+CGG 0.585455 8_1:+78604009 Msa1216690:CDS
TGCTGTGTGAAATCTTTGGA+TGG 0.594368 8_1:+78603506 Msa1216690:CDS
AGAGAAACAGAGGAACATGA+AGG 0.596213 8_1:-78602992 None:intergenic
CCGAAATGGTGTCTGATCAG+AGG 0.599821 8_1:-78604041 None:intergenic
ACAACCAAATATATAGTGAC+CGG 0.600585 8_1:-78606444 None:intergenic
AGTATAGTATAGTATAGCAT+AGG 0.601271 8_1:+78603441 Msa1216690:five_prime_UTR
ACCACTTAGGAGAAACTACA+AGG 0.602100 8_1:-78606368 None:intergenic
CAGGATCCTTCACCAATTCA+GGG 0.602285 8_1:-78603826 None:intergenic
AATTTGGAGTTACAATGCAA+AGG 0.605004 8_1:+78603348 Msa1216690:five_prime_UTR
TTTGCTTATCCAGCGAATCA+GGG 0.607172 8_1:-78603661 None:intergenic
TCCAAAACAAAGTTGTGTGA+AGG 0.609730 8_1:-78603608 None:intergenic
GCTGTGATAGCTGTTAAGGG+TGG 0.610038 8_1:+78603992 Msa1216690:CDS
CAATTAAGTGAGGTCAATGT+CGG 0.610601 8_1:+78606053 Msa1216690:CDS
TAACGACCCTGAATTGGTGA+AGG 0.614068 8_1:+78603820 Msa1216690:CDS
ATGATAAACATCAAATTCAG+TGG 0.614811 8_1:-78606120 None:intergenic
TGCATTTACAAGGAGTTTAG+GGG 0.615174 8_1:+78604225 Msa1216690:CDS
TTGATAGCAACAAACAAATG+GGG 0.616102 8_1:-78603249 None:intergenic
AGAGTCATATAAACTATGGT+CGG 0.617426 8_1:+78605447 Msa1216690:CDS
AACATAGAAATGTACCACTT+AGG 0.617985 8_1:-78606381 None:intergenic
TCGGTACTGCTATGAGAACC+AGG 0.619300 8_1:+78606072 Msa1216690:CDS
TAGCTGTTAAGGGTGGGGAT+CGG 0.622651 8_1:+78603999 Msa1216690:CDS
ACTGCTATGAGAACCAGGAC+AGG 0.627745 8_1:+78606077 Msa1216690:CDS
AGATCCTCGTGATGCATGTG+CGG 0.629182 8_1:+78605414 Msa1216690:CDS
TAACCATAAAGAATCTCACC+TGG 0.646025 8_1:+78606627 Msa1216690:three_prime_UTR
GACATTGATGATTCTATGAG+TGG 0.646804 8_1:+78603902 Msa1216690:CDS
AAGACAATGAACCTCATACC+CGG 0.647246 8_1:-78606476 None:intergenic
ATGGTGATCCTCCGAGATTG+TGG 0.650626 8_1:+78604176 Msa1216690:CDS
TGGGGATCGGATTATTGCGG+AGG 0.654548 8_1:+78604012 Msa1216690:CDS
GGTAACGAAGAGAGTTGGGA+TGG 0.656385 8_1:+78604157 Msa1216690:CDS
TGATTCTATGAGTGGTACAA+CGG 0.665201 8_1:+78603910 Msa1216690:CDS
TGTGTGAAATCTTTGGATGG+CGG 0.665816 8_1:+78603509 Msa1216690:CDS
GGAGAAACTACAAGGAGACC+TGG 0.666616 8_1:-78606360 None:intergenic
ATCTGGAGTTCAATTAAGTG+AGG 0.669700 8_1:+78606043 Msa1216690:CDS
TTTACAGGGAACAAAGAGAG+GGG 0.669983 8_1:+78606322 Msa1216690:intron
CCTTCAAAGAAGAGAAACAG+AGG 0.673946 8_1:-78603002 None:intergenic
TGGTGTGGTTGCAACTCCAG+AGG 0.684152 8_1:+78604267 Msa1216690:CDS
TATACCTTTCTCACACTGCG+AGG 0.685306 8_1:+78603632 Msa1216690:CDS
ATAGCCGCACATGCATCACG+AGG 0.723344 8_1:-78605418 None:intergenic
ATTGGCGGAGACAATTGGTG+TGG 0.737589 8_1:+78604252 Msa1216690:CDS
TTTGGGAACCACAATCTCGG+AGG 0.737719 8_1:-78604184 None:intergenic
GATCCAAATATCCAACACTG+GGG 0.772205 8_1:+78604136 Msa1216690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAATTATAAAGTAAAAT+TGG - chr8_1:78604849-78604868 None:intergenic 10.0%
!!! TTTTAGATTTGTATTATTAA+TGG + chr8_1:78605612-78605631 Msa1216690:intron 10.0%
!!! CAATATATAATTTCTATTTT+TGG - chr8_1:78605763-78605782 None:intergenic 10.0%
!! CAAAAATAGAAATTATATAT+TGG + chr8_1:78605761-78605780 Msa1216690:intron 10.0%
!! ATTTCTAATAAGTAGATAAT+AGG - chr8_1:78604654-78604673 None:intergenic 15.0%
!! ATTATAAAGTAAAATTGGAA+CGG - chr8_1:78604844-78604863 None:intergenic 15.0%
!! AAGTTAATACAAATGATAAA+TGG - chr8_1:78605123-78605142 None:intergenic 15.0%
!!! TTATCATTTGTATTAACTTT+TGG + chr8_1:78605124-78605143 Msa1216690:intron 15.0%
!!! TATTTTATATTGTTTGATCA+TGG + chr8_1:78606263-78606282 Msa1216690:intron 15.0%
!!! AATTTGATCCTTTTGAAATT+GGG - chr8_1:78603332-78603351 None:intergenic 20.0%
!!! AAATTTGATCCTTTTGAAAT+TGG - chr8_1:78603333-78603352 None:intergenic 20.0%
!! AATTTCAAAAGGATCAAATT+TGG + chr8_1:78603332-78603351 Msa1216690:five_prime_UTR 20.0%
!! ATTGAAAAAAACAACTTTAC+AGG - chr8_1:78603385-78603404 None:intergenic 20.0%
!!! GAAGAAAAATCTTTACATTT+TGG + chr8_1:78604528-78604547 Msa1216690:intron 20.0%
!! TATATATATATATATCCCCA+TGG + chr8_1:78604929-78604948 Msa1216690:intron 20.0%
!! ATATATATATATATCCCCAT+GGG + chr8_1:78604930-78604949 Msa1216690:intron 20.0%
!!! CTAAAATTTTAAAACAAGCT+TGG - chr8_1:78605598-78605617 None:intergenic 20.0%
!! AAAAAAATTCCTAAAGTCTA+TGG - chr8_1:78605712-78605731 None:intergenic 20.0%
!!! TCAATAAATACTTGCTATTT+GGG + chr8_1:78605829-78605848 Msa1216690:intron 20.0%
!!! AAGTAACTAGAATTTTCTTT+TGG - chr8_1:78606568-78606587 None:intergenic 20.0%
!! AGCAAACAATAATTAAAAAC+TGG + chr8_1:78606724-78606743 Msa1216690:three_prime_UTR 20.0%
!! GCAAACAATAATTAAAAACT+GGG + chr8_1:78606725-78606744 Msa1216690:three_prime_UTR 20.0%
! AAAAAAAAAAGAACCCTAGT+AGG - chr8_1:78603169-78603188 None:intergenic 25.0%
!! TTTGATAGCAACAAACAAAT+GGG - chr8_1:78603253-78603272 None:intergenic 25.0%
!! ATTTGATAGCAACAAACAAA+TGG - chr8_1:78603254-78603273 None:intergenic 25.0%
!! AAGTTGCTAATTTTGTAACT+AGG + chr8_1:78603292-78603311 Msa1216690:five_prime_UTR 25.0%
!! AGTTGCTAATTTTGTAACTA+GGG + chr8_1:78603293-78603312 Msa1216690:five_prime_UTR 25.0%
! AGTATAGTATAGTATAGCAT+AGG + chr8_1:78603441-78603460 Msa1216690:five_prime_UTR 25.0%
!!! ATTTCTTTGGTGTTTTTGAT+GGG + chr8_1:78603735-78603754 Msa1216690:CDS 25.0%
! AAACTTTGTTAGAGATAGTT+TGG + chr8_1:78603784-78603803 Msa1216690:CDS 25.0%
! ACAATAAAGAAGAGATGATT+AGG - chr8_1:78604313-78604332 None:intergenic 25.0%
! CTAGAAAAAAAAACTAAGAG+AGG - chr8_1:78604401-78604420 None:intergenic 25.0%
! CATTTAGATAATATCATCGT+AGG + chr8_1:78604599-78604618 Msa1216690:intron 25.0%
! GCAATTATGTTAATGGTTTA+TGG + chr8_1:78604754-78604773 Msa1216690:intron 25.0%
! GTTAATGGTTTATGGTTATT+CGG + chr8_1:78604762-78604781 Msa1216690:intron 25.0%
! TATATATATATATCCCCATG+GGG + chr8_1:78604931-78604950 Msa1216690:intron 25.0%
! GTAATATACAAACATAGGTT+AGG + chr8_1:78604953-78604972 Msa1216690:intron 25.0%
! AATTGTTTGTCACAATCATA+TGG + chr8_1:78605052-78605071 Msa1216690:intron 25.0%
! ATTGTTTGTCACAATCATAT+GGG + chr8_1:78605053-78605072 Msa1216690:intron 25.0%
! TAACTTCCAATTAAACGAAT+TGG - chr8_1:78605201-78605220 None:intergenic 25.0%
!!! TGTGTTTTATTTGATTCTGT+AGG + chr8_1:78605378-78605397 Msa1216690:intron 25.0%
! ATTATCATTGTCCAGATTAA+AGG + chr8_1:78605499-78605518 Msa1216690:CDS 25.0%
!! TAAAAGGAAAATGGTAATGA+AGG - chr8_1:78605563-78605582 None:intergenic 25.0%
! AACTACACATAAAAGGAAAA+TGG - chr8_1:78605572-78605591 None:intergenic 25.0%
!!! GATAATACCTTGTTTTTCAT+GGG - chr8_1:78605648-78605667 None:intergenic 25.0%
!!! CTCAATAAATACTTGCTATT+TGG + chr8_1:78605828-78605847 Msa1216690:intron 25.0%
!!! CAATAAATACTTGCTATTTG+GGG + chr8_1:78605830-78605849 Msa1216690:intron 25.0%
!! CTCTACATGTTTTTCTTTTT+GGG - chr8_1:78605896-78605915 None:intergenic 25.0%
! AATTTGAATCAACTCATCAT+TGG + chr8_1:78605975-78605994 Msa1216690:intron 25.0%
! ATGATAAACATCAAATTCAG+TGG - chr8_1:78606123-78606142 None:intergenic 25.0%
! ACTATATATTTGGTTGTTCA+AGG + chr8_1:78606450-78606469 Msa1216690:three_prime_UTR 25.0%
! TAATTTCTACTTTCTGAACA+AGG - chr8_1:78606517-78606536 None:intergenic 25.0%
!!! TTTTTTTTTCTTTCCAGAGA+GGG - chr8_1:78606602-78606621 None:intergenic 25.0%
!!! ATTTTTTTTTCTTTCCAGAG+AGG - chr8_1:78606603-78606622 None:intergenic 25.0%
AAAAAAGTAGTACCTTTCGT+GGG - chr8_1:78603043-78603062 None:intergenic 30.0%
AAAAAAAGTAGTACCTTTCG+TGG - chr8_1:78603044-78603063 None:intergenic 30.0%
!! GTAGAAACTGTTTGATTTTG+GGG - chr8_1:78603135-78603154 None:intergenic 30.0%
!! GGTAGAAACTGTTTGATTTT+GGG - chr8_1:78603136-78603155 None:intergenic 30.0%
! TTGATAGCAACAAACAAATG+GGG - chr8_1:78603252-78603271 None:intergenic 30.0%
ACATGATACCCAATTTCAAA+AGG + chr8_1:78603321-78603340 Msa1216690:five_prime_UTR 30.0%
! AATTTGGAGTTACAATGCAA+AGG + chr8_1:78603348-78603367 Msa1216690:five_prime_UTR 30.0%
! ATATCACCTGTTTGAAACAT+GGG + chr8_1:78603464-78603483 Msa1216690:exon 30.0%
! ACCAAGATAGTTTTTGCATT+AGG + chr8_1:78603684-78603703 Msa1216690:CDS 30.0%
TCCTAATGCAAAAACTATCT+TGG - chr8_1:78603688-78603707 None:intergenic 30.0%
! AGATAGTTTTTGCATTAGGA+CGG + chr8_1:78603688-78603707 Msa1216690:CDS 30.0%
ACACCAAAGAAATGGATATT+CGG - chr8_1:78603728-78603747 None:intergenic 30.0%
CATCAAAAACACCAAAGAAA+TGG - chr8_1:78603736-78603755 None:intergenic 30.0%
!!! CATTTCTTTGGTGTTTTTGA+TGG + chr8_1:78603734-78603753 Msa1216690:CDS 30.0%
ACCAACATTAGCAACATAAA+GGG - chr8_1:78603963-78603982 None:intergenic 30.0%
TTCTTTATTGTTGCTAGTGA+TGG + chr8_1:78604322-78604341 Msa1216690:CDS 30.0%
ATACCATATCAACAACAGTT+TGG - chr8_1:78604369-78604388 None:intergenic 30.0%
!! TTACATTTTGGCCACAATTA+TGG + chr8_1:78604540-78604559 Msa1216690:intron 30.0%
GAAATCGTATCATCTATCAA+TGG + chr8_1:78604669-78604688 Msa1216690:intron 30.0%
!! AAGACTTTGACTTGAAAATC+AGG + chr8_1:78604709-78604728 Msa1216690:intron 30.0%
TCTGTGTGCAATTATGTTAA+TGG + chr8_1:78604747-78604766 Msa1216690:intron 30.0%
ATGTTTGTATATTACCCCAT+GGG - chr8_1:78604948-78604967 None:intergenic 30.0%
TATGTTTGTATATTACCCCA+TGG - chr8_1:78604949-78604968 None:intergenic 30.0%
ATGGGGTAATATACAAACAT+AGG + chr8_1:78604948-78604967 Msa1216690:intron 30.0%
! TTCCTTGTTTAGCATAACTA+TGG + chr8_1:78604976-78604995 Msa1216690:intron 30.0%
AACCATAGTTATGCTAAACA+AGG - chr8_1:78604981-78605000 None:intergenic 30.0%
ATAACTATGGTTCATTTACC+AGG + chr8_1:78604989-78605008 Msa1216690:intron 30.0%
ATTATATTTGCACAACTACC+TGG - chr8_1:78605010-78605029 None:intergenic 30.0%
CCTAATTAACAGATGGAATT+TGG - chr8_1:78605095-78605114 None:intergenic 30.0%
CCAAATTCCATCTGTTAATT+AGG + chr8_1:78605092-78605111 Msa1216690:intron 30.0%
CAATTAAACGAATTGGCAAT+TGG - chr8_1:78605194-78605213 None:intergenic 30.0%
CAATTGCCAATTCGTTTAAT+TGG + chr8_1:78605192-78605211 Msa1216690:intron 30.0%
!!! ACTGTGTAGAATTTTAACTC+TGG + chr8_1:78605328-78605347 Msa1216690:intron 30.0%
ACATTCATCAAGATAAGAAG+GGG - chr8_1:78605361-78605380 None:intergenic 30.0%
CACATTCATCAAGATAAGAA+GGG - chr8_1:78605362-78605381 None:intergenic 30.0%
ACACATTCATCAAGATAAGA+AGG - chr8_1:78605363-78605382 None:intergenic 30.0%
CTGAAGAGTCATATAAACTA+TGG + chr8_1:78605443-78605462 Msa1216690:CDS 30.0%
AGAGTCATATAAACTATGGT+CGG + chr8_1:78605447-78605466 Msa1216690:CDS 30.0%
TATTAGAGAGTCCTTTAATC+TGG - chr8_1:78605513-78605532 None:intergenic 30.0%
AAGGACTCTCTAATAATGTA+TGG + chr8_1:78605518-78605537 Msa1216690:intron 30.0%
TTGGAGAAACTACACATAAA+AGG - chr8_1:78605579-78605598 None:intergenic 30.0%
TTCTTATCCCATGAAAAACA+AGG + chr8_1:78605638-78605657 Msa1216690:intron 30.0%
!!! GGATAATACCTTGTTTTTCA+TGG - chr8_1:78605649-78605668 None:intergenic 30.0%
! AAGGTATTATCCCATCAAAT+GGG + chr8_1:78605657-78605676 Msa1216690:intron 30.0%
TAATTCACTCCATAGACTTT+AGG + chr8_1:78605700-78605719 Msa1216690:intron 30.0%
! GCTCTACATGTTTTTCTTTT+TGG - chr8_1:78605897-78605916 None:intergenic 30.0%
CAAAATCAAAATCTTGCTAC+TGG + chr8_1:78605943-78605962 Msa1216690:intron 30.0%
TTATTCAACTCATCTTTGAC+TGG - chr8_1:78606181-78606200 None:intergenic 30.0%
GTAAAGAAAATAGGAGGAAA+AGG - chr8_1:78606307-78606326 None:intergenic 30.0%
! TTTCCTCCTATTTTCTTTAC+AGG + chr8_1:78606307-78606326 Msa1216690:intron 30.0%
! TTCCTCCTATTTTCTTTACA+GGG + chr8_1:78606308-78606327 Msa1216690:intron 30.0%
TTGTTCCCTGTAAAGAAAAT+AGG - chr8_1:78606316-78606335 None:intergenic 30.0%
AACATAGAAATGTACCACTT+AGG - chr8_1:78606384-78606403 None:intergenic 30.0%
TTTACCGGTCACTATATATT+TGG + chr8_1:78606440-78606459 Msa1216690:three_prime_UTR 30.0%
ACAACCAAATATATAGTGAC+CGG - chr8_1:78606447-78606466 None:intergenic 30.0%
CAGAAAGTAGAAATTAGAGT+TGG + chr8_1:78606521-78606540 Msa1216690:three_prime_UTR 30.0%
GATACAATAAAACATTCTCC+AGG - chr8_1:78606648-78606667 None:intergenic 30.0%
!!! AATTTTGGAATGAGAGAGAC+AGG - chr8_1:78602925-78602944 None:intergenic 35.0%
!! CAAGTTGACGTGAAGAATTT+TGG - chr8_1:78602940-78602959 None:intergenic 35.0%
TTGACTCTTCCTTCTTCATT+AGG + chr8_1:78602964-78602983 Msa1216690:five_prime_UTR 35.0%
TGACTCTTCCTTCTTCATTA+GGG + chr8_1:78602965-78602984 Msa1216690:five_prime_UTR 35.0%
AAGGAAAACCCTAATGAAGA+AGG - chr8_1:78602976-78602995 None:intergenic 35.0%
AAAAAGTAGTACCTTTCGTG+GGG - chr8_1:78603042-78603061 None:intergenic 35.0%
GAAAATGCAATGAAGGTTCT+AGG - chr8_1:78603075-78603094 None:intergenic 35.0%
CAGACAAGAAAATGCAATGA+AGG - chr8_1:78603082-78603101 None:intergenic 35.0%
! TTTGAGAAAAAGGTGCAAGT+GGG - chr8_1:78603108-78603127 None:intergenic 35.0%
!! GGGTAGAAACTGTTTGATTT+TGG - chr8_1:78603137-78603156 None:intergenic 35.0%
!!! TACCCTTTTTTCTACCTACT+AGG + chr8_1:78603152-78603171 Msa1216690:five_prime_UTR 35.0%
!!! ACCCTTTTTTCTACCTACTA+GGG + chr8_1:78603153-78603172 Msa1216690:five_prime_UTR 35.0%
ACCCTAGTAGGTAGAAAAAA+GGG - chr8_1:78603157-78603176 None:intergenic 35.0%
AACCCTAGTAGGTAGAAAAA+AGG - chr8_1:78603158-78603177 None:intergenic 35.0%
GAGTCAAATTTGAGCTCAAT+AGG - chr8_1:78603199-78603218 None:intergenic 35.0%
! GATATCACCTGTTTGAAACA+TGG + chr8_1:78603463-78603482 Msa1216690:exon 35.0%
ATGTTGCTGTGTGAAATCTT+TGG + chr8_1:78603502-78603521 Msa1216690:CDS 35.0%
TTGGTTATCATCATAGCCAT+AGG + chr8_1:78603543-78603562 Msa1216690:CDS 35.0%
!! TCCTTCACACAACTTTGTTT+TGG + chr8_1:78603607-78603626 Msa1216690:CDS 35.0%
TCCAAAACAAAGTTGTGTGA+AGG - chr8_1:78603611-78603630 None:intergenic 35.0%
AACCCGAATATCCATTTCTT+TGG + chr8_1:78603722-78603741 Msa1216690:CDS 35.0%
CACCAAAGAAATGGATATTC+GGG - chr8_1:78603727-78603746 None:intergenic 35.0%
! GACATTGATGATTCTATGAG+TGG + chr8_1:78603902-78603921 Msa1216690:CDS 35.0%
TGATTCTATGAGTGGTACAA+CGG + chr8_1:78603910-78603929 Msa1216690:CDS 35.0%
ACCCTTTATGTTGCTAATGT+TGG + chr8_1:78603959-78603978 Msa1216690:CDS 35.0%
CACCAACATTAGCAACATAA+AGG - chr8_1:78603964-78603983 None:intergenic 35.0%
ACTGCATTTACAAGGAGTTT+AGG + chr8_1:78604223-78604242 Msa1216690:CDS 35.0%
CTGCATTTACAAGGAGTTTA+GGG + chr8_1:78604224-78604243 Msa1216690:CDS 35.0%
TGCATTTACAAGGAGTTTAG+GGG + chr8_1:78604225-78604244 Msa1216690:CDS 35.0%
AAGCCAAACTGTTGTTGATA+TGG + chr8_1:78604363-78604382 Msa1216690:CDS 35.0%
GTTGATATTGTGAATGACTG+TGG + chr8_1:78604460-78604479 Msa1216690:intron 35.0%
! TTTGGCCACAATTATGGTTT+TGG + chr8_1:78604546-78604565 Msa1216690:intron 35.0%
TGTTTGTATATTACCCCATG+GGG - chr8_1:78604947-78604966 None:intergenic 35.0%
GGATAGACCTAATTAACAGA+TGG - chr8_1:78605102-78605121 None:intergenic 35.0%
!!! TGTAGAATTTTAACTCTGGC+AGG + chr8_1:78605332-78605351 Msa1216690:intron 35.0%
! CAAGGTATTATCCCATCAAA+TGG + chr8_1:78605656-78605675 Msa1216690:intron 35.0%
AGGTATTATCCCATCAAATG+GGG + chr8_1:78605658-78605677 Msa1216690:intron 35.0%
AATCCTTAACATGTGTCCTT+AGG + chr8_1:78605853-78605872 Msa1216690:intron 35.0%
ATCCTTAACATGTGTCCTTA+GGG + chr8_1:78605854-78605873 Msa1216690:intron 35.0%
! ATCTGGAGTTCAATTAAGTG+AGG + chr8_1:78606043-78606062 Msa1216690:CDS 35.0%
CAATTAAGTGAGGTCAATGT+CGG + chr8_1:78606053-78606072 Msa1216690:CDS 35.0%
TTCCCTGTAAAGAAAATAGG+AGG - chr8_1:78606313-78606332 None:intergenic 35.0%
TGCAAAAGACTCAGAAAAGT+TGG - chr8_1:78606407-78606426 None:intergenic 35.0%
!!! TCTTTTGGTTTCATGTGTCT+TGG - chr8_1:78606553-78606572 None:intergenic 35.0%
TAACCATAAAGAATCTCACC+TGG + chr8_1:78606627-78606646 Msa1216690:three_prime_UTR 35.0%
AGAGAAACAGAGGAACATGA+AGG - chr8_1:78602995-78603014 None:intergenic 40.0%
CCTTCAAAGAAGAGAAACAG+AGG - chr8_1:78603005-78603024 None:intergenic 40.0%
! CCTCTGTTTCTCTTCTTTGA+AGG + chr8_1:78603002-78603021 Msa1216690:five_prime_UTR 40.0%
! CTTTGAGAAAAAGGTGCAAG+TGG - chr8_1:78603109-78603128 None:intergenic 40.0%
! TTGGGGTGACTTTGAGAAAA+AGG - chr8_1:78603118-78603137 None:intergenic 40.0%
ACACAACCCATGTTTCAAAC+AGG - chr8_1:78603473-78603492 None:intergenic 40.0%
! TGAAACATGGGTTGTGTTCA+TGG + chr8_1:78603476-78603495 Msa1216690:exon 40.0%
TGCTGTGTGAAATCTTTGGA+TGG + chr8_1:78603506-78603525 Msa1216690:CDS 40.0%
TGTGTGAAATCTTTGGATGG+CGG + chr8_1:78603509-78603528 Msa1216690:CDS 40.0%
GAGCTTGTATGTTCTTCCTA+TGG - chr8_1:78603562-78603581 None:intergenic 40.0%
TTTGCTTATCCAGCGAATCA+GGG - chr8_1:78603664-78603683 None:intergenic 40.0%
! TTTTGCTTATCCAGCGAATC+AGG - chr8_1:78603665-78603684 None:intergenic 40.0%
TGCATTAGGACGGAAATTCA+AGG + chr8_1:78603698-78603717 Msa1216690:CDS 40.0%
!!! TTTGGTGTTTTTGATGGGCA+TGG + chr8_1:78603740-78603759 Msa1216690:CDS 40.0%
! TTTGATGGGCATGGTCAATT+TGG + chr8_1:78603749-78603768 Msa1216690:CDS 40.0%
GTTATCTAACGACCCTGAAT+TGG + chr8_1:78603814-78603833 Msa1216690:CDS 40.0%
AGCTTACAATTCTGCGTTCT+CGG + chr8_1:78603853-78603872 Msa1216690:CDS 40.0%
!! GCTATTACAGTGCTTGTTGT+TGG + chr8_1:78603932-78603951 Msa1216690:CDS 40.0%
AGAGCTGTGATAGCTGTTAA+GGG + chr8_1:78603989-78604008 Msa1216690:CDS 40.0%
TCGTATTCATCTCTCCGAAA+TGG - chr8_1:78604058-78604077 None:intergenic 40.0%
TACGAGAGAGTGAAACTTTG+TGG + chr8_1:78604073-78604092 Msa1216690:CDS 40.0%
AGTGAAACTTTGTGGAGCAA+GGG + chr8_1:78604081-78604100 Msa1216690:CDS 40.0%
!!! GGTTTTGAGTGTTGATCAAG+TGG + chr8_1:78604102-78604121 Msa1216690:CDS 40.0%
!! TTGAGTGTTGATCAAGTGGA+AGG + chr8_1:78604106-78604125 Msa1216690:CDS 40.0%
!! TGAGTGTTGATCAAGTGGAA+GGG + chr8_1:78604107-78604126 Msa1216690:CDS 40.0%
TGATCAAGTGGAAGGGATTA+AGG + chr8_1:78604114-78604133 Msa1216690:CDS 40.0%
AGGATCCAAATATCCAACAC+TGG + chr8_1:78604134-78604153 Msa1216690:CDS 40.0%
GGATCCAAATATCCAACACT+GGG + chr8_1:78604135-78604154 Msa1216690:CDS 40.0%
GATCCAAATATCCAACACTG+GGG + chr8_1:78604136-78604155 Msa1216690:CDS 40.0%
! TTACCCCAGTGTTGGATATT+TGG - chr8_1:78604142-78604161 None:intergenic 40.0%
TTCCAGGAAACATCCCATTT+GGG - chr8_1:78604204-78604223 None:intergenic 40.0%
TTCCTGGAACTGCATTTACA+AGG + chr8_1:78604215-78604234 Msa1216690:CDS 40.0%
AAGGAGTTTAGGGGATAGAT+TGG + chr8_1:78604234-78604253 Msa1216690:CDS 40.0%
TTGTGAATGACTGTGGACAA+AGG + chr8_1:78604467-78604486 Msa1216690:intron 40.0%
TGTCTGATGCTGCCAAATTA+TGG + chr8_1:78604490-78604509 Msa1216690:intron 40.0%
GCAGTCCAAAACCATAATTG+TGG - chr8_1:78604554-78604573 None:intergenic 40.0%
AATTGGAACGGTATTTCGCT+TGG - chr8_1:78604832-78604851 None:intergenic 40.0%
!! TACAGTATTGCAACGGGTTA+GGG - chr8_1:78605273-78605292 None:intergenic 40.0%
AGTGTCTACAGTATTGCAAC+GGG - chr8_1:78605279-78605298 None:intergenic 40.0%
CAGTGTCTACAGTATTGCAA+CGG - chr8_1:78605280-78605299 None:intergenic 40.0%
TTCTACACAGTAATGCAGCA+GGG - chr8_1:78605319-78605338 None:intergenic 40.0%
ATTCTACACAGTAATGCAGC+AGG - chr8_1:78605320-78605339 None:intergenic 40.0%
ACATTGTAGTCAAGCACATC+TGG + chr8_1:78606026-78606045 Msa1216690:intron 40.0%
TCTTTACAGGGAACAAAGAG+AGG + chr8_1:78606320-78606339 Msa1216690:intron 40.0%
CTTTACAGGGAACAAAGAGA+GGG + chr8_1:78606321-78606340 Msa1216690:intron 40.0%
TTTACAGGGAACAAAGAGAG+GGG + chr8_1:78606322-78606341 Msa1216690:intron 40.0%
! GAACAAAGAGAGGGGTTTTA+CGG + chr8_1:78606330-78606349 Msa1216690:three_prime_UTR 40.0%
TCCTTGTAGTTTCTCCTAAG+TGG + chr8_1:78606367-78606386 Msa1216690:three_prime_UTR 40.0%
ACCACTTAGGAGAAACTACA+AGG - chr8_1:78606371-78606390 None:intergenic 40.0%
CATTGCTCCTGTACATTTAC+CGG + chr8_1:78606425-78606444 Msa1216690:three_prime_UTR 40.0%
ATAGTGACCGGTAAATGTAC+AGG - chr8_1:78606435-78606454 None:intergenic 40.0%
AAGACAATGAACCTCATACC+CGG - chr8_1:78606479-78606498 None:intergenic 40.0%
CATTGTCTTGGCAGCAAAAA+TGG + chr8_1:78606490-78606509 Msa1216690:three_prime_UTR 40.0%
! TTTTCTTTCCAGAGAGGGTA+TGG - chr8_1:78606597-78606616 None:intergenic 40.0%
TCTCCAGGTGAGATTCTTTA+TGG - chr8_1:78606633-78606652 None:intergenic 40.0%
AATGAGAGAGACAGGAGAAG+TGG - chr8_1:78602917-78602936 None:intergenic 45.0%
TATACCTTTCTCACACTGCG+AGG + chr8_1:78603632-78603651 Msa1216690:CDS 45.0%
TAACGACCCTGAATTGGTGA+AGG + chr8_1:78603820-78603839 Msa1216690:CDS 45.0%
CAGGATCCTTCACCAATTCA+GGG - chr8_1:78603829-78603848 None:intergenic 45.0%
ACAGGATCCTTCACCAATTC+AGG - chr8_1:78603830-78603849 None:intergenic 45.0%
GCAGAATTGTAAGCTCGAAC+AGG - chr8_1:78603848-78603867 None:intergenic 45.0%
GAGAGCTGTGATAGCTGTTA+AGG + chr8_1:78603988-78604007 Msa1216690:CDS 45.0%
CTGTGATAGCTGTTAAGGGT+GGG + chr8_1:78603993-78604012 Msa1216690:CDS 45.0%
TGTGATAGCTGTTAAGGGTG+GGG + chr8_1:78603994-78604013 Msa1216690:CDS 45.0%
CCTCTGATCAGACACCATTT+CGG + chr8_1:78604041-78604060 Msa1216690:CDS 45.0%
GAGTGAAACTTTGTGGAGCA+AGG + chr8_1:78604080-78604099 Msa1216690:CDS 45.0%
CCATTTGGGAACCACAATCT+CGG - chr8_1:78604190-78604209 None:intergenic 45.0%
CGAGATTGTGGTTCCCAAAT+GGG + chr8_1:78604188-78604207 Msa1216690:CDS 45.0%
TTCCCAAATGGGATGTTTCC+TGG + chr8_1:78604199-78604218 Msa1216690:CDS 45.0%
GTTCCAGGAAACATCCCATT+TGG - chr8_1:78604205-78604224 None:intergenic 45.0%
CTCCTTGTAAATGCAGTTCC+AGG - chr8_1:78604220-78604239 None:intergenic 45.0%
GAGTTTAGGGGATAGATTGG+CGG + chr8_1:78604237-78604256 Msa1216690:CDS 45.0%
GATAGATTGGCGGAGACAAT+TGG + chr8_1:78604247-78604266 Msa1216690:CDS 45.0%
AGCTGAACTATTGACACCTC+TGG - chr8_1:78604286-78604305 None:intergenic 45.0%
ATGCTGCCAAATTATGGCTG+TGG + chr8_1:78604496-78604515 Msa1216690:intron 45.0%
ACCACACCACAGCCATAATT+TGG - chr8_1:78604505-78604524 None:intergenic 45.0%
! CTACAGTATTGCAACGGGTT+AGG - chr8_1:78605274-78605293 None:intergenic 45.0%
TTATCCCATCAAATGGGGTC+AGG + chr8_1:78605663-78605682 Msa1216690:intron 45.0%
TATCCCATCAAATGGGGTCA+GGG + chr8_1:78605664-78605683 Msa1216690:intron 45.0%
AGCCCTAAGGACACATGTTA+AGG - chr8_1:78605859-78605878 None:intergenic 45.0%
CAATGCTAACATGAGCCCTA+AGG - chr8_1:78605872-78605891 None:intergenic 45.0%
CTTAGGGCTCATGTTAGCAT+TGG + chr8_1:78605870-78605889 Msa1216690:intron 45.0%
TCAACTCATCTTTGACTGGC+AGG - chr8_1:78606177-78606196 None:intergenic 45.0%
ATTTGGTTGTTCAAGGCAGC+CGG + chr8_1:78606457-78606476 Msa1216690:three_prime_UTR 45.0%
GGGTATGAGGTTCATTGTCT+TGG + chr8_1:78606478-78606497 Msa1216690:three_prime_UTR 45.0%
TTGTTTAGCCATACCCTCTC+TGG + chr8_1:78606586-78606605 Msa1216690:three_prime_UTR 45.0%
!!! TAATACATAATTTTTTTTAT+TGG - chr8_1:78605740-78605759 None:intergenic 5.0%
AGTAGTACCTTTCGTGGGGT+GGG - chr8_1:78603038-78603057 None:intergenic 50.0%
AAGTAGTACCTTTCGTGGGG+TGG - chr8_1:78603039-78603058 None:intergenic 50.0%
!! GATGGCGGCTCTAGAAAACT+TGG + chr8_1:78603524-78603543 Msa1216690:CDS 50.0%
GTAACCTCGCAGTGTGAGAA+AGG - chr8_1:78603639-78603658 None:intergenic 50.0%
! AGGTTACTACCCTGATTCGC+TGG + chr8_1:78603652-78603671 Msa1216690:CDS 50.0%
GCTGTGATAGCTGTTAAGGG+TGG + chr8_1:78603992-78604011 Msa1216690:CDS 50.0%
TAGCTGTTAAGGGTGGGGAT+CGG + chr8_1:78603999-78604018 Msa1216690:CDS 50.0%
CCGAAATGGTGTCTGATCAG+AGG - chr8_1:78604044-78604063 None:intergenic 50.0%
TCTCTTCGTTACCCCAGTGT+TGG - chr8_1:78604150-78604169 None:intergenic 50.0%
ACTGGGGTAACGAAGAGAGT+TGG + chr8_1:78604152-78604171 Msa1216690:CDS 50.0%
CTGGGGTAACGAAGAGAGTT+GGG + chr8_1:78604153-78604172 Msa1216690:CDS 50.0%
GGTAACGAAGAGAGTTGGGA+TGG + chr8_1:78604157-78604176 Msa1216690:CDS 50.0%
ATGGTGATCCTCCGAGATTG+TGG + chr8_1:78604176-78604195 Msa1216690:CDS 50.0%
TTTGGGAACCACAATCTCGG+AGG - chr8_1:78604187-78604206 None:intergenic 50.0%
CCGAGATTGTGGTTCCCAAA+TGG + chr8_1:78604187-78604206 Msa1216690:CDS 50.0%
ATTGGCGGAGACAATTGGTG+TGG + chr8_1:78604252-78604271 Msa1216690:CDS 50.0%
GCCAAATTATGGCTGTGGTG+TGG + chr8_1:78604501-78604520 Msa1216690:intron 50.0%
AGATCCTCGTGATGCATGTG+CGG + chr8_1:78605414-78605433 Msa1216690:CDS 50.0%
ATCCCATCAAATGGGGTCAG+GGG + chr8_1:78605665-78605684 Msa1216690:intron 50.0%
TTCCCCTGACCCCATTTGAT+GGG - chr8_1:78605670-78605689 None:intergenic 50.0%
ATTCCCCTGACCCCATTTGA+TGG - chr8_1:78605671-78605690 None:intergenic 50.0%
TCGGTACTGCTATGAGAACC+AGG + chr8_1:78606072-78606091 Msa1216690:CDS 50.0%
ACTGCTATGAGAACCAGGAC+AGG + chr8_1:78606077-78606096 Msa1216690:CDS 50.0%
CATCTCAGAAGTTCCTGTCC+TGG - chr8_1:78606093-78606112 None:intergenic 50.0%
GGAGAAACTACAAGGAGACC+TGG - chr8_1:78606363-78606382 None:intergenic 50.0%
TTTGGTTGTTCAAGGCAGCC+GGG + chr8_1:78606458-78606477 Msa1216690:three_prime_UTR 50.0%
GTAGTACCTTTCGTGGGGTG+GGG - chr8_1:78603037-78603056 None:intergenic 55.0%
GGGTGGGGATCGGATTATTG+CGG + chr8_1:78604009-78604028 Msa1216690:CDS 55.0%
TGGGGATCGGATTATTGCGG+AGG + chr8_1:78604012-78604031 Msa1216690:CDS 55.0%
TGGTGTGGTTGCAACTCCAG+AGG + chr8_1:78604267-78604286 Msa1216690:CDS 55.0%
! AAACTTGCCCTGCTTGCTGC+TGG + chr8_1:78605243-78605262 Msa1216690:intron 55.0%
ATAGCCGCACATGCATCACG+AGG - chr8_1:78605421-78605440 None:intergenic 55.0%
! GGGTTTTACGGAAGCTCACC+AGG + chr8_1:78606342-78606361 Msa1216690:three_prime_UTR 55.0%
GGGATGACCAGCAGCAAGCA+GGG - chr8_1:78605253-78605272 None:intergenic 60.0%
AGGGATGACCAGCAGCAAGC+AGG - chr8_1:78605254-78605273 None:intergenic 60.0%
GTTCAAGGCAGCCGGGTATG+AGG + chr8_1:78606465-78606484 Msa1216690:three_prime_UTR 60.0%
ACTGCACCCCACCCCACGAA+AGG + chr8_1:78603028-78603047 Msa1216690:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 78602885 78606762 78602885 ID=Msa1216690;Name=Msa1216690
chr8_1 mRNA 78602885 78606762 78602885 ID=Msa1216690-mRNA-1;Parent=Msa1216690;Name=Msa1216690-mRNA-1;_AED=0.03;_eAED=0.03;_QI=597|1|1|1|0.66|0.5|4|438|399
chr8_1 exon 78602885 78604384 78602885 ID=Msa1216690-mRNA-1:exon:27191;Parent=Msa1216690-mRNA-1
chr8_1 exon 78605400 78605534 78605400 ID=Msa1216690-mRNA-1:exon:27192;Parent=Msa1216690-mRNA-1
chr8_1 exon 78606035 78606200 78606035 ID=Msa1216690-mRNA-1:exon:27193;Parent=Msa1216690-mRNA-1
chr8_1 exon 78606329 78606762 78606329 ID=Msa1216690-mRNA-1:exon:27194;Parent=Msa1216690-mRNA-1
chr8_1 five_prime_UTR 78602885 78603481 78602885 ID=Msa1216690-mRNA-1:five_prime_utr;Parent=Msa1216690-mRNA-1
chr8_1 CDS 78603482 78604384 78603482 ID=Msa1216690-mRNA-1:cds;Parent=Msa1216690-mRNA-1
chr8_1 CDS 78605400 78605534 78605400 ID=Msa1216690-mRNA-1:cds;Parent=Msa1216690-mRNA-1
chr8_1 CDS 78606035 78606196 78606035 ID=Msa1216690-mRNA-1:cds;Parent=Msa1216690-mRNA-1
chr8_1 three_prime_UTR 78606197 78606200 78606197 ID=Msa1216690-mRNA-1:three_prime_utr;Parent=Msa1216690-mRNA-1
chr8_1 three_prime_UTR 78606329 78606762 78606329 ID=Msa1216690-mRNA-1:three_prime_utr;Parent=Msa1216690-mRNA-1
Gene Sequence

>Msa1216690

ATGGGTTGTGTTCATGGTAAATGTTGCTGTGTGAAATCTTTGGATGGCGGCTCTAGAAAACTTGGTTATCATCATAGCCATAGGAAGAACATACAAGCTCAAAGAGTATTGAAAAATGTTTATGTTCCTTCACACAACTTTGTTTTGGAGTATACCTTTCTCACACTGCGAGGTTACTACCCTGATTCGCTGGATAAGCAAAACCAAGATAGTTTTTGCATTAGGACGGAAATTCAAGGTAACCCGAATATCCATTTCTTTGGTGTTTTTGATGGGCATGGTCAATTTGGTAGTCAATGTTCAAACTTTGTTAGAGATAGTTTGGTAGAGAAGTTATCTAACGACCCTGAATTGGTGAAGGATCCTGTTCGAGCTTACAATTCTGCGTTCTCGGCAACAAATCACGAATTGCATACTAGTGACATTGATGATTCTATGAGTGGTACAACGGCTATTACAGTGCTTGTTGTTGGAGACACCCTTTATGTTGCTAATGTTGGTGATTCGAGAGCTGTGATAGCTGTTAAGGGTGGGGATCGGATTATTGCGGAGGACTTGTCCTCTGATCAGACACCATTTCGGAGAGATGAATACGAGAGAGTGAAACTTTGTGGAGCAAGGGTTTTGAGTGTTGATCAAGTGGAAGGGATTAAGGATCCAAATATCCAACACTGGGGTAACGAAGAGAGTTGGGATGGTGATCCTCCGAGATTGTGGTTCCCAAATGGGATGTTTCCTGGAACTGCATTTACAAGGAGTTTAGGGGATAGATTGGCGGAGACAATTGGTGTGGTTGCAACTCCAGAGGTGTCAATAGTTCAGCTTACACCTAATCATCTCTTCTTTATTGTTGCTAGTGATGGCATATTTGAATTCTTGTCAAGCCAAACTGTTGTTGATATGGCAGCAAGATATACAGATCCTCGTGATGCATGTGCGGCTATTGCTGAAGAGTCATATAAACTATGGTCGGAACTTGTGAATCGAACAGATGATATAACAATTATCATTGTCCAGATTAAAGGACTCTCTAATAATTCAAGCACATCTGGAGTTCAATTAAGTGAGGTCAATGTCGGTACTGCTATGAGAACCAGGACAGGAACTTCTGAGATGTCTACTACCACTGAATTTGATGTTTATCATTCTGTGACGAACAGTTTCTCTGATTCACAATCCTGCCAGTCAAAGATGAGTTGA
Protein sequence

>Msa1216690

MGCVHGKCCCVKSLDGGSRKLGYHHSHRKNIQAQRVLKNVYVPSHNFVLEYTFLTLRGYYPDSLDKQNQDSFCIRTEIQGNPNIHFFGVFDGHGQFGSQCSNFVRDSLVEKLSNDPELVKDPVRAYNSAFSATNHELHTSDIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAVKGGDRIIAEDLSSDQTPFRRDEYERVKLCGARVLSVDQVEGIKDPNIQHWGNEESWDGDPPRLWFPNGMFPGTAFTRSLGDRLAETIGVVATPEVSIVQLTPNHLFFIVASDGIFEFLSSQTVVDMAARYTDPRDACAAIAEESYKLWSELVNRTDDITIIIVQIKGLSNNSSTSGVQLSEVNVGTAMRTRTGTSEMSTTTEFDVYHSVTNSFSDSQSCQSKMS*