Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410760 | B7FH61 | 88.889 | 135 | 13 | 1 | 1 | 133 | 1 | 135 | 1.11e-76 | 233 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029540 | Msa1410760 | 0.811707 | 6.117802e-51 | -8.615850e-47 |
| Msa0032090 | Msa1410760 | -0.822485 | 2.309696e-53 | -8.615850e-47 |
| Msa0040690 | Msa1410760 | -0.803220 | 3.868030e-49 | -8.615850e-47 |
| Msa0044540 | Msa1410760 | 0.821639 | 3.627206e-53 | -8.615850e-47 |
| Msa0074950 | Msa1410760 | 0.812589 | 3.929186e-51 | -8.615850e-47 |
| Msa0079380 | Msa1410760 | -0.809385 | 1.942001e-50 | -8.615850e-47 |
| Msa0085360 | Msa1410760 | 0.803279 | 3.760414e-49 | -8.615850e-47 |
| Msa0091210 | Msa1410760 | 0.803476 | 3.423989e-49 | -8.615850e-47 |
| Msa0091250 | Msa1410760 | 0.820791 | 5.692126e-53 | -8.615850e-47 |
| Msa1364470 | Msa1410760 | 0.810327 | 1.217996e-50 | -8.615850e-47 |
| Msa1374120 | Msa1410760 | -0.813560 | 2.406166e-51 | -8.615850e-47 |
| Msa1376090 | Msa1410760 | 0.819279 | 1.262282e-52 | -8.615850e-47 |
| Msa1377540 | Msa1410760 | 0.803104 | 4.087564e-49 | -8.615850e-47 |
| Msa1400610 | Msa1410760 | 0.823519 | 1.326276e-53 | -8.615850e-47 |
| Msa1410430 | Msa1410760 | 0.827515 | 1.499425e-54 | -8.615850e-47 |
| Msa1410760 | Msa1429360 | 0.830899 | 2.264421e-55 | -8.615850e-47 |
| Msa1410760 | Msa1449250 | 0.815832 | 7.552546e-52 | -8.615850e-47 |
| Msa0661190 | Msa1410760 | 0.810034 | 1.408983e-50 | -8.615850e-47 |
| Msa0698490 | Msa1410760 | -0.802137 | 6.471133e-49 | -8.615850e-47 |
| Msa0698660 | Msa1410760 | -0.805927 | 1.054100e-49 | -8.615850e-47 |
| Msa0701860 | Msa1410760 | 0.821409 | 4.100253e-53 | -8.615850e-47 |
| Msa0754720 | Msa1410760 | 0.812432 | 4.253193e-51 | -8.615850e-47 |
| Msa0756630 | Msa1410760 | 0.835458 | 1.658337e-56 | -8.615850e-47 |
| Msa0796530 | Msa1410760 | -0.806893 | 6.592310e-50 | -8.615850e-47 |
| Msa0138970 | Msa1410760 | 0.802045 | 6.759073e-49 | -8.615850e-47 |
| Msa0143240 | Msa1410760 | 0.802200 | 6.280940e-49 | -8.615850e-47 |
| Msa0156650 | Msa1410760 | 0.826536 | 2.570356e-54 | -8.615850e-47 |
| Msa0183300 | Msa1410760 | 0.808724 | 2.691105e-50 | -8.615850e-47 |
| Msa0215320 | Msa1410760 | 0.807697 | 4.452922e-50 | -8.615850e-47 |
| Msa0225740 | Msa1410760 | -0.800490 | 1.406681e-48 | -8.615850e-47 |
| Msa0226470 | Msa1410760 | -0.807121 | 5.901573e-50 | -8.615850e-47 |
| Msa0229490 | Msa1410760 | 0.816632 | 5.003787e-52 | -8.615850e-47 |
| Msa1245940 | Msa1410760 | 0.829709 | 4.423318e-55 | -8.615850e-47 |
| Msa1253930 | Msa1410760 | 0.824770 | 6.739820e-54 | -8.615850e-47 |
| Msa1259070 | Msa1410760 | 0.834823 | 2.397758e-56 | -8.615850e-47 |
| Msa1264020 | Msa1410760 | -0.808347 | 3.238754e-50 | -8.615850e-47 |
| Msa1269580 | Msa1410760 | 0.837643 | 4.604270e-57 | -8.615850e-47 |
| Msa1280420 | Msa1410760 | -0.801011 | 1.101205e-48 | -8.615850e-47 |
| Msa1284260 | Msa1410760 | -0.803151 | 3.997652e-49 | -8.615850e-47 |
| Msa1307660 | Msa1410760 | 0.833192 | 6.139839e-56 | -8.615850e-47 |
| Msa1343070 | Msa1410760 | -0.801914 | 7.190312e-49 | -8.615850e-47 |
| Msa0386920 | Msa1410760 | -0.809785 | 1.593329e-50 | -8.615850e-47 |
| Msa0414950 | Msa1410760 | 0.835273 | 1.846922e-56 | -8.615850e-47 |
| Msa0421780 | Msa1410760 | 0.821875 | 3.199563e-53 | -8.615850e-47 |
| Msa0436280 | Msa1410760 | 0.832748 | 7.919587e-56 | -8.615850e-47 |
| Msa0459770 | Msa1410760 | 0.822634 | 2.132360e-53 | -8.615850e-47 |
| Msa0467960 | Msa1410760 | 0.800778 | 1.228654e-48 | -8.615850e-47 |
| Msa0981820 | Msa1410760 | 0.828963 | 6.710725e-55 | -8.615850e-47 |
| Msa1004900 | Msa1410760 | 0.807394 | 5.164971e-50 | -8.615850e-47 |
| Msa1007610 | Msa1410760 | 0.806681 | 7.310020e-50 | -8.615850e-47 |
| Msa1079370 | Msa1410760 | 0.810230 | 1.278097e-50 | -8.615850e-47 |
| Msa1081560 | Msa1410760 | -0.801095 | 1.058253e-48 | -8.615850e-47 |
| Msa0260950 | Msa1410760 | 0.815306 | 9.892672e-52 | -8.615850e-47 |
| Msa0272480 | Msa1410760 | 0.844091 | 9.372148e-59 | -8.615850e-47 |
| Msa0294560 | Msa1410760 | 0.830043 | 3.668019e-55 | -8.615850e-47 |
| Msa0312690 | Msa1410760 | 0.839879 | 1.216757e-57 | -8.615850e-47 |
| Msa1127130 | Msa1410760 | -0.811810 | 5.810367e-51 | -8.615850e-47 |
| Msa1208890 | Msa1410760 | 0.815958 | 7.079369e-52 | -8.615850e-47 |
| Msa1217860 | Msa1410760 | -0.801924 | 7.157079e-49 | -8.615850e-47 |
| Msa0511310 | Msa1410760 | -0.809108 | 2.226706e-50 | -8.615850e-47 |
| Msa0574760 | Msa1410760 | 0.805119 | 1.556752e-49 | -8.615850e-47 |
| Msa0579390 | Msa1410760 | -0.800851 | 1.187264e-48 | -8.615850e-47 |
| Msa0850960 | Msa1410760 | -0.839285 | 1.736773e-57 | -8.615850e-47 |
| Msa0867050 | Msa1410760 | 0.828107 | 1.080492e-54 | -8.615850e-47 |
| Msa0920610 | Msa1410760 | -0.802674 | 5.016335e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410760 | MtrunA17_Chr1g0197561 | 88.889 | 135 | 13 | 1 | 1 | 133 | 1 | 135 | 2.13e-80 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGGCGGAGATTCGCAGTTT+TGG | 0.368913 | tig0023698:+80168 | None:intergenic |
| TTCTTGTAAGAGGAAGCTTA+TGG | 0.390438 | tig0023698:-79911 | Msa1410760:CDS |
| AGCCGTTTGTTTCTTGTAAG+AGG | 0.398994 | tig0023698:-79921 | Msa1410760:CDS |
| GACATCGTGTCGGCAACGTC+AGG | 0.417129 | tig0023698:+80096 | None:intergenic |
| AAGCAAGATACCGGAGATAC+AGG | 0.441445 | tig0023698:-79971 | Msa1410760:CDS |
| TTACAAGAAACAAACGGCTT+CGG | 0.447163 | tig0023698:+79925 | None:intergenic |
| TTTCTTTCGACGTCGGAGTC+CGG | 0.456673 | tig0023698:+79992 | None:intergenic |
| GTGAAATCGATGAGAGTTAC+CGG | 0.456812 | tig0023698:-80011 | Msa1410760:CDS |
| ACAAGTTCTCTTCGGAAAAG+TGG | 0.496245 | tig0023698:+79831 | None:intergenic |
| GTACAAGGACAAGTTCTCTT+CGG | 0.515314 | tig0023698:+79823 | None:intergenic |
| GGCGGACAATCCTGTATCTC+CGG | 0.535721 | tig0023698:+79961 | None:intergenic |
| TTCCTCTTACAAGAAACAAA+CGG | 0.536839 | tig0023698:+79919 | None:intergenic |
| ATCTTGCTTTCTTTCGACGT+CGG | 0.541967 | tig0023698:+79985 | None:intergenic |
| GTCGAAAGAAAGCAAGATAC+CGG | 0.550012 | tig0023698:-79980 | Msa1410760:CDS |
| ATTGAGAAAGAAAACGTTGA+CGG | 0.551569 | tig0023698:-80048 | Msa1410760:CDS |
| GTTGACGGTGCTGTTGTTGA+CGG | 0.553828 | tig0023698:-80033 | Msa1410760:CDS |
| GTCGTTCTCGATGACGTCGA+CGG | 0.585755 | tig0023698:+80149 | None:intergenic |
| TCAGGGACTTGATCGGAGGA+AGG | 0.617553 | tig0023698:+80114 | None:intergenic |
| TAATTGCTGGAGGTGAAGAT+CGG | 0.620642 | tig0023698:+80191 | None:intergenic |
| GGCAACGTCAGGGACTTGAT+CGG | 0.627390 | tig0023698:+80107 | None:intergenic |
| ACATCGTGTCGGCAACGTCA+GGG | 0.631276 | tig0023698:+80097 | None:intergenic |
| TGCCGACGACGACATCGTGT+CGG | 0.644546 | tig0023698:+80086 | None:intergenic |
| GGAGATACAGGATTGTCCGC+CGG | 0.647121 | tig0023698:-79959 | Msa1410760:CDS |
| GAGGTGAAGATCGGTAGACA+TGG | 0.661979 | tig0023698:+80200 | None:intergenic |
| AACGTCAGGGACTTGATCGG+AGG | 0.670622 | tig0023698:+80110 | None:intergenic |
| GTTCTCGATGACGTCGACGG+CGG | 0.690884 | tig0023698:+80152 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGAGAAAGAAAACGTTGA+CGG | - | tig0023698:79969-79988 | Msa1410760:CDS | 30.0% | |
| TTCCTCTTACAAGAAACAAA+CGG | + | tig0023698:80101-80120 | None:intergenic | 30.0% | |
| TTACAAGAAACAAACGGCTT+CGG | + | tig0023698:80095-80114 | None:intergenic | 35.0% | |
| TTCTTGTAAGAGGAAGCTTA+TGG | - | tig0023698:80106-80125 | Msa1410760:CDS | 35.0% | |
| TAATTGCTGGAGGTGAAGAT+CGG | + | tig0023698:79829-79848 | None:intergenic | 40.0% | |
| !! | TTCGCAGTTTTGGTAATTGC+TGG | + | tig0023698:79842-79861 | None:intergenic | 40.0% |
| GTGAAATCGATGAGAGTTAC+CGG | - | tig0023698:80006-80025 | Msa1410760:CDS | 40.0% | |
| ATCTTGCTTTCTTTCGACGT+CGG | + | tig0023698:80035-80054 | None:intergenic | 40.0% | |
| GTCGAAAGAAAGCAAGATAC+CGG | - | tig0023698:80037-80056 | Msa1410760:CDS | 40.0% | |
| ! | AGCCGTTTGTTTCTTGTAAG+AGG | - | tig0023698:80096-80115 | Msa1410760:CDS | 40.0% |
| ACAAGTTCTCTTCGGAAAAG+TGG | + | tig0023698:80189-80208 | None:intergenic | 40.0% | |
| GTACAAGGACAAGTTCTCTT+CGG | + | tig0023698:80197-80216 | None:intergenic | 40.0% | |
| !! | GCAGTTTTGGTAATTGCTGG+AGG | + | tig0023698:79839-79858 | None:intergenic | 45.0% |
| AAGCAAGATACCGGAGATAC+AGG | - | tig0023698:80046-80065 | Msa1410760:CDS | 45.0% | |
| ! | ACAAACGGCTTCGGTTTTCT+CGG | + | tig0023698:80086-80105 | None:intergenic | 45.0% |
| !! | GTTGACGGTGCTGTTGTTGA+CGG | - | tig0023698:79984-80003 | Msa1410760:CDS | 50.0% |
| TTTCTTTCGACGTCGGAGTC+CGG | + | tig0023698:80028-80047 | None:intergenic | 50.0% | |
| ! | ACGGCGGAGATTCGCAGTTT+TGG | + | tig0023698:79852-79871 | None:intergenic | 55.0% |
| GTCGTTCTCGATGACGTCGA+CGG | + | tig0023698:79871-79890 | None:intergenic | 55.0% | |
| TCAGGGACTTGATCGGAGGA+AGG | + | tig0023698:79906-79925 | None:intergenic | 55.0% | |
| AACGTCAGGGACTTGATCGG+AGG | + | tig0023698:79910-79929 | None:intergenic | 55.0% | |
| GGCAACGTCAGGGACTTGAT+CGG | + | tig0023698:79913-79932 | None:intergenic | 55.0% | |
| ! | ACATCGTGTCGGCAACGTCA+GGG | + | tig0023698:79923-79942 | None:intergenic | 55.0% |
| GGCGGACAATCCTGTATCTC+CGG | + | tig0023698:80059-80078 | None:intergenic | 55.0% | |
| GGAGATACAGGATTGTCCGC+CGG | - | tig0023698:80058-80077 | Msa1410760:CDS | 55.0% | |
| GTTCTCGATGACGTCGACGG+CGG | + | tig0023698:79868-79887 | None:intergenic | 60.0% | |
| ! | GACATCGTGTCGGCAACGTC+AGG | + | tig0023698:79924-79943 | None:intergenic | 60.0% |
| TGCCGACACGATGTCGTCGT+CGG | - | tig0023698:79929-79948 | Msa1410760:CDS | 60.0% | |
| TGCCGACGACGACATCGTGT+CGG | + | tig0023698:79934-79953 | None:intergenic | 60.0% | |
| !! | TTCGGTTTTCTCGGCGCCGG+CGG | + | tig0023698:80077-80096 | None:intergenic | 65.0% |
| ! | GGCTTCGGTTTTCTCGGCGC+CGG | + | tig0023698:80080-80099 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023698 | gene | 79819 | 80220 | 79819 | ID=Msa1410760;Name=Msa1410760 |
| tig0023698 | mRNA | 79819 | 80220 | 79819 | ID=Msa1410760-mRNA-1;Parent=Msa1410760;Name=Msa1410760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|133 |
| tig0023698 | exon | 79819 | 80220 | 79819 | ID=Msa1410760-mRNA-1:exon:26690;Parent=Msa1410760-mRNA-1 |
| tig0023698 | CDS | 79819 | 80220 | 79819 | ID=Msa1410760-mRNA-1:cds;Parent=Msa1410760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
