AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022086

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022086.t1 MTR_7g077620 67.385 371 119 2 1 371 1 369 5.37e-170 479
MS.gene022086.t1 MTR_7g077650 64.211 380 122 5 1 374 1 372 4.24e-159 451
MS.gene022086.t1 MTR_7g077660 63.564 376 127 5 1 374 1 368 8.80e-153 435
MS.gene022086.t1 MTR_7g076110 54.882 297 123 6 85 374 21 313 9.23e-98 293
MS.gene022086.t1 MTR_7g076100 63.291 237 85 2 138 374 66 300 6.24e-93 280
MS.gene022086.t1 MTR_7g076100 78.000 50 11 0 4 53 5 54 2.35e-20 90.5
MS.gene022086.t1 MTR_1g108740 45.669 381 161 15 1 372 1 344 4.31e-87 267
MS.gene022086.t1 MTR_5g038260 40.648 401 188 15 3 374 4 383 4.81e-77 243
MS.gene022086.t1 MTR_5g082770 46.624 311 131 8 5 306 4 288 2.19e-72 228
MS.gene022086.t1 MTR_5g038230 39.594 394 168 15 5 372 4 353 8.89e-72 228
MS.gene022086.t1 MTR_1g064570 38.902 419 189 16 3 374 2 400 1.16e-67 219
MS.gene022086.t1 MTR_7g076130 68.800 125 39 0 167 291 24 148 8.59e-53 172
MS.gene022086.t1 MTR_1g016150 50.265 189 87 5 1 184 1 187 4.07e-49 166
MS.gene022086.t1 MTR_1g016150 61.538 65 19 2 256 320 177 235 9.56e-13 67.8
MS.gene022086.t1 MTR_5g082780 40.167 239 99 12 119 339 6 218 1.49e-33 125
MS.gene022086.t1 MTR_1g108750 44.231 156 51 5 5 159 4 124 1.93e-22 92.0
MS.gene022086.t1 MTR_3g114970 24.543 383 207 18 1 351 1 333 8.44e-13 69.3
MS.gene022086.t1 MTR_3g114990 24.181 397 219 17 3 351 21 383 3.12e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCAAGCCAATCATTT+TGG 0.214143 7.1:-77656650 MS.gene022086:CDS
TTCTCATAGCAAGTAAAATT+AGG 0.229231 7.1:+77656495 None:intergenic
AGGAGGATTATTGAACTTAA+AGG 0.273304 7.1:+77656536 None:intergenic
AATGAACAAACTTGAGAGTT+TGG 0.291432 7.1:-77656289 MS.gene022086:CDS
TACTCACTTGGGCTTGTAAT+CGG 0.312123 7.1:+77656714 None:intergenic
AAGAACACATAGATCATGTT+AGG 0.348741 7.1:-77657097 MS.gene022086:CDS
TTCGAGAGGTGGCAAACTAA+TGG 0.352493 7.1:+77656767 None:intergenic
GATCTTTACCCCGTCCCTTC+AGG 0.356454 7.1:-77656104 MS.gene022086:CDS
AACGACCTTCACCATAGCTT+TGG 0.364212 7.1:+77656442 None:intergenic
CAAGAACATGAGTGTGTGTT+TGG 0.364316 7.1:-77656871 MS.gene022086:CDS
AGAAGCCTATCCTCTTAGTT+TGG 0.365278 7.1:-77657135 MS.gene022086:CDS
TGATGTTTACAAATTAAAAC+TGG 0.368827 7.1:-77656337 MS.gene022086:CDS
CAAGGATGGGATAACTGAAT+AGG 0.377070 7.1:+77656897 None:intergenic
TAGCAATGGGGAAGTTAGAT+TGG 0.377310 7.1:-77657166 None:intergenic
TCTCATAGCAAGTAAAATTA+GGG 0.387493 7.1:+77656496 None:intergenic
TCGAGAGGTGGCAAACTAAT+GGG 0.396706 7.1:+77656768 None:intergenic
CACATAGATCATGTTAGGTT+TGG 0.400301 7.1:-77657092 MS.gene022086:CDS
GGGTTCTACCACAATTTCAA+TGG 0.408660 7.1:-77656065 MS.gene022086:CDS
ACGACCTTCACCATAGCTTT+GGG 0.423915 7.1:+77656443 None:intergenic
CGCCTCTGCTTTCTCCAAGA+TGG 0.446470 7.1:-77656217 MS.gene022086:CDS
ACTCACTTGGGCTTGTAATC+GGG 0.449180 7.1:+77656715 None:intergenic
AAGGATGGGATAACTGAATA+GGG 0.462191 7.1:+77656898 None:intergenic
TTGGCGTGGTGTGCAAGAAT+TGG 0.475029 7.1:-77657073 MS.gene022086:CDS
GGTTCTACCACAATTTCAAT+GGG 0.480142 7.1:-77656064 MS.gene022086:CDS
CTGGTATTTGCTGACAACCT+TGG 0.497796 7.1:-77656573 MS.gene022086:CDS
AGCAATGGGGAAGTTAGATT+GGG 0.499131 7.1:-77657165 None:intergenic
AAGAACATGAGTGTGTGTTT+GGG 0.500810 7.1:-77656870 MS.gene022086:CDS
TCATGTTCTTGATGCAAGGA+TGG 0.501083 7.1:+77656883 None:intergenic
TAGTTTGGTACTTGATAAGT+TGG 0.501505 7.1:-77657120 MS.gene022086:CDS
GTGTGTTTGGGCTTTAGCCA+TGG 0.507415 7.1:-77656858 MS.gene022086:CDS
TAGCATCTTCCTGAAGGGAC+GGG 0.508712 7.1:+77656095 None:intergenic
CTACACGGATCCAAAATGAT+TGG 0.509049 7.1:+77656640 None:intergenic
CCTTCACCATAGCTTTGGGC+TGG 0.509210 7.1:+77656447 None:intergenic
GTTTGGGCTTTAGCCATGGT+TGG 0.511722 7.1:-77656854 MS.gene022086:CDS
TTATCAAGTACCAAACTAAG+AGG 0.513488 7.1:+77657125 None:intergenic
AATCGTTGAATGGAGACATA+TGG 0.523073 7.1:-77656410 MS.gene022086:CDS
CATATCTATAATGCGGAGGA+TGG 0.531607 7.1:-77656141 MS.gene022086:CDS
AGGATACAATGGTGCGTCCA+AGG 0.537916 7.1:+77656556 None:intergenic
GCTGCAACCACATACTCACT+TGG 0.539884 7.1:+77656702 None:intergenic
GTTGATGAAAGTGATAAAGA+TGG 0.555094 7.1:-77656174 MS.gene022086:CDS
AAAATGTAGCATCTTCCTGA+AGG 0.561599 7.1:+77656089 None:intergenic
AAGTACCAAACTAAGAGGAT+AGG 0.568196 7.1:+77657130 None:intergenic
AGTGTTCCAGCCCAAAGCTA+TGG 0.568256 7.1:-77656453 MS.gene022086:CDS
GTAGCATCTTCCTGAAGGGA+CGG 0.568660 7.1:+77656094 None:intergenic
TACAAGCCCAAGTGAGTATG+TGG 0.569105 7.1:-77656709 MS.gene022086:CDS
GGTCAATCCCATTGAAATTG+TGG 0.574786 7.1:+77656057 None:intergenic
AGCATCTTCCTGAAGGGACG+GGG 0.577537 7.1:+77656096 None:intergenic
GAGAGGATCATCAACATAGG+TGG 0.578281 7.1:+77656999 None:intergenic
ACCTCTCGAAAATATCAAGA+AGG 0.578390 7.1:-77656754 MS.gene022086:CDS
TGTAATCGGGTCAGCAGACA+AGG 0.580068 7.1:+77656728 None:intergenic
TACGATAATTTATGTATGAG+AGG 0.585202 7.1:+77656982 None:intergenic
ACACTCATGTTCTTGATGCA+AGG 0.588895 7.1:+77656879 None:intergenic
ATTGAACTTAAAGGATACAA+TGG 0.590271 7.1:+77656545 None:intergenic
CATGTTCTTGATGCAAGGAT+GGG 0.593302 7.1:+77656884 None:intergenic
GTTGCAGCGATTTACAATTG+TGG 0.603650 7.1:-77656687 MS.gene022086:CDS
AAATGTAGCATCTTCCTGAA+GGG 0.613321 7.1:+77656090 None:intergenic
TTCCATCTTGGAGAAAGCAG+AGG 0.613967 7.1:+77656215 None:intergenic
AGAGGATCATCAACATAGGT+GGG 0.618694 7.1:+77657000 None:intergenic
TATGAGAGGATCATCAACAT+AGG 0.620354 7.1:+77656996 None:intergenic
CTGCAACCACATACTCACTT+GGG 0.622251 7.1:+77656703 None:intergenic
AGATCATGTTAGGTTTGGCG+TGG 0.628602 7.1:-77657087 MS.gene022086:CDS
CCAGCCCAAAGCTATGGTGA+AGG 0.629782 7.1:-77656447 MS.gene022086:CDS
CATCTTGGAGAAAGCAGAGG+CGG 0.635809 7.1:+77656218 None:intergenic
GGGTCATTTGAGTCATCACA+AGG 0.644746 7.1:+77656516 None:intergenic
TGAGTTACATATCTATAATG+CGG 0.657813 7.1:-77656148 MS.gene022086:CDS
GTGTAGACATAAATGAATTG+TGG 0.661724 7.1:-77656623 MS.gene022086:CDS
ACAATTCATTTATGTCTACA+CGG 0.669650 7.1:+77656625 None:intergenic
GTTACATATCTATAATGCGG+AGG 0.671372 7.1:-77656145 MS.gene022086:CDS
TTAAAACTGGATGCCCAGAG+TGG 0.678752 7.1:-77656324 MS.gene022086:CDS
GTTGAATGGAGACATATGGA+TGG 0.713125 7.1:-77656406 MS.gene022086:CDS
TCATTTGAGTCATCACAAGG+AGG 0.722989 7.1:+77656519 None:intergenic
GTAAACATCAAAATTATACG+AGG 0.728568 7.1:+77656350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAATGTTGTATTTTATAA+AGG - chr7.1:77656623-77656642 MS.gene022086:CDS 15.0%
!! ACCATAAAAATCATCAATTT+AGG - chr7.1:77656189-77656208 MS.gene022086:CDS 20.0%
!! TGATGTTTACAAATTAAAAC+TGG - chr7.1:77656883-77656902 MS.gene022086:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr7.1:77656193-77656212 None:intergenic 20.0%
! AAAAAGTTTCACTAGTGTTA+TGG + chr7.1:77656417-77656436 None:intergenic 25.0%
! ACAATTCATTTATGTCTACA+CGG + chr7.1:77656598-77656617 None:intergenic 25.0%
! ATTGAACTTAAAGGATACAA+TGG + chr7.1:77656678-77656697 None:intergenic 25.0%
! GTAAACATCAAAATTATACG+AGG + chr7.1:77656873-77656892 None:intergenic 25.0%
! TACGATAATTTATGTATGAG+AGG + chr7.1:77656241-77656260 None:intergenic 25.0%
! TCTCATAGCAAGTAAAATTA+GGG + chr7.1:77656727-77656746 None:intergenic 25.0%
! TGAGTTACATATCTATAATG+CGG - chr7.1:77657072-77657091 MS.gene022086:CDS 25.0%
! TTCTCATAGCAAGTAAAATT+AGG + chr7.1:77656728-77656747 None:intergenic 25.0%
!! AATTGAATTCTTTTCCATCT+TGG + chr7.1:77657020-77657039 None:intergenic 25.0%
!! TATTTTGTGAAATCGTTGAA+TGG - chr7.1:77656800-77656819 MS.gene022086:CDS 25.0%
!! TGTTGTATTTTATAAAGGTC+TGG - chr7.1:77656628-77656647 MS.gene022086:CDS 25.0%
!!! ATTTTATTTGTCGGAAACTT+TGG - chr7.1:77656962-77656981 MS.gene022086:CDS 25.0%
!!! GAAGACAACATTTTATTTGT+CGG - chr7.1:77656953-77656972 MS.gene022086:CDS 25.0%
AAGAACACATAGATCATGTT+AGG - chr7.1:77656123-77656142 MS.gene022086:CDS 30.0%
AATGAACAAACTTGAGAGTT+TGG - chr7.1:77656931-77656950 MS.gene022086:CDS 30.0%
AGGAGGATTATTGAACTTAA+AGG + chr7.1:77656687-77656706 None:intergenic 30.0%
GTGTAGACATAAATGAATTG+TGG - chr7.1:77656597-77656616 MS.gene022086:CDS 30.0%
TAGTTTGGTACTTGATAAGT+TGG - chr7.1:77656100-77656119 MS.gene022086:CDS 30.0%
TTATCAAGTACCAAACTAAG+AGG + chr7.1:77656098-77656117 None:intergenic 30.0%
! GTTGATGAAAGTGATAAAGA+TGG - chr7.1:77657046-77657065 MS.gene022086:CDS 30.0%
AAAATGTAGCATCTTCCTGA+AGG + chr7.1:77657134-77657153 None:intergenic 35.0%
AAATGTAGCATCTTCCTGAA+GGG + chr7.1:77657133-77657152 None:intergenic 35.0%
AAGAACATGAGTGTGTGTTT+GGG - chr7.1:77656350-77656369 MS.gene022086:CDS 35.0%
AAGGATGGGATAACTGAATA+GGG + chr7.1:77656325-77656344 None:intergenic 35.0%
AAGTACCAAACTAAGAGGAT+AGG + chr7.1:77656093-77656112 None:intergenic 35.0%
AATCGTTGAATGGAGACATA+TGG - chr7.1:77656810-77656829 MS.gene022086:CDS 35.0%
ACCTCTCGAAAATATCAAGA+AGG - chr7.1:77656466-77656485 MS.gene022086:CDS 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.1:77656128-77656147 MS.gene022086:CDS 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr7.1:77657156-77657175 MS.gene022086:CDS 35.0%
GTTACATATCTATAATGCGG+AGG - chr7.1:77657075-77657094 MS.gene022086:CDS 35.0%
TATGAGAGGATCATCAACAT+AGG + chr7.1:77656227-77656246 None:intergenic 35.0%
! AAAGAGCAAGCCAATCATTT+TGG - chr7.1:77656570-77656589 MS.gene022086:CDS 35.0%
! CAGGAAGATGCTACATTTTT+GGG - chr7.1:77657135-77657154 MS.gene022086:CDS 35.0%
! TCAGGAAGATGCTACATTTT+TGG - chr7.1:77657134-77657153 MS.gene022086:CDS 35.0%
!! ACCTTCTTGATATTTTCGAG+AGG + chr7.1:77656470-77656489 None:intergenic 35.0%
!! ATTTTAATGCAAGCCAACCA+TGG + chr7.1:77656382-77656401 None:intergenic 35.0%
!! TTCTTGATATTTTCGAGAGG+TGG + chr7.1:77656467-77656486 None:intergenic 35.0%
!! TTGTTCAAGTTTTCCACTCT+GGG + chr7.1:77656912-77656931 None:intergenic 35.0%
!! TTTGTTCAAGTTTTCCACTC+TGG + chr7.1:77656913-77656932 None:intergenic 35.0%
ACACTCATGTTCTTGATGCA+AGG + chr7.1:77656344-77656363 None:intergenic 40.0%
AGAAGCCTATCCTCTTAGTT+TGG - chr7.1:77656085-77656104 MS.gene022086:CDS 40.0%
AGAGGATCATCAACATAGGT+GGG + chr7.1:77656223-77656242 None:intergenic 40.0%
CAAGAACATGAGTGTGTGTT+TGG - chr7.1:77656349-77656368 MS.gene022086:CDS 40.0%
CAAGGATGGGATAACTGAAT+AGG + chr7.1:77656326-77656345 None:intergenic 40.0%
CATATCTATAATGCGGAGGA+TGG - chr7.1:77657079-77657098 MS.gene022086:CDS 40.0%
CATGTTCTTGATGCAAGGAT+GGG + chr7.1:77656339-77656358 None:intergenic 40.0%
CTACACGGATCCAAAATGAT+TGG + chr7.1:77656583-77656602 None:intergenic 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr7.1:77657155-77657174 MS.gene022086:CDS 40.0%
GTTGAATGGAGACATATGGA+TGG - chr7.1:77656814-77656833 MS.gene022086:CDS 40.0%
GTTGCAGCGATTTACAATTG+TGG - chr7.1:77656533-77656552 MS.gene022086:CDS 40.0%
TACTCACTTGGGCTTGTAAT+CGG + chr7.1:77656509-77656528 None:intergenic 40.0%
TCATGTTCTTGATGCAAGGA+TGG + chr7.1:77656340-77656359 None:intergenic 40.0%
TCATTTGAGTCATCACAAGG+AGG + chr7.1:77656704-77656723 None:intergenic 40.0%
AACGACCTTCACCATAGCTT+TGG + chr7.1:77656781-77656800 None:intergenic 45.0%
ACGACCTTCACCATAGCTTT+GGG + chr7.1:77656780-77656799 None:intergenic 45.0%
ACTCACTTGGGCTTGTAATC+GGG + chr7.1:77656508-77656527 None:intergenic 45.0%
AGATCATGTTAGGTTTGGCG+TGG - chr7.1:77656133-77656152 MS.gene022086:CDS 45.0%
CTGCAACCACATACTCACTT+GGG + chr7.1:77656520-77656539 None:intergenic 45.0%
GAGAGGATCATCAACATAGG+TGG + chr7.1:77656224-77656243 None:intergenic 45.0%
GGGTCATTTGAGTCATCACA+AGG + chr7.1:77656707-77656726 None:intergenic 45.0%
TACAAGCCCAAGTGAGTATG+TGG - chr7.1:77656511-77656530 MS.gene022086:CDS 45.0%
TCGAGAGGTGGCAAACTAAT+GGG + chr7.1:77656455-77656474 None:intergenic 45.0%
TTAAAACTGGATGCCCAGAG+TGG - chr7.1:77656896-77656915 MS.gene022086:CDS 45.0%
TTCCATCTTGGAGAAAGCAG+AGG + chr7.1:77657008-77657027 None:intergenic 45.0%
TTCGAGAGGTGGCAAACTAA+TGG + chr7.1:77656456-77656475 None:intergenic 45.0%
! CTGGTATTTGCTGACAACCT+TGG - chr7.1:77656647-77656666 MS.gene022086:CDS 45.0%
AGGATACAATGGTGCGTCCA+AGG + chr7.1:77656667-77656686 None:intergenic 50.0%
AGTGTTCCAGCCCAAAGCTA+TGG - chr7.1:77656767-77656786 MS.gene022086:CDS 50.0%
CATCTTGGAGAAAGCAGAGG+CGG + chr7.1:77657005-77657024 None:intergenic 50.0%
GCTGCAACCACATACTCACT+TGG + chr7.1:77656521-77656540 None:intergenic 50.0%
GTAGCATCTTCCTGAAGGGA+CGG + chr7.1:77657129-77657148 None:intergenic 50.0%
TAGCATCTTCCTGAAGGGAC+GGG + chr7.1:77657128-77657147 None:intergenic 50.0%
TGTAATCGGGTCAGCAGACA+AGG + chr7.1:77656495-77656514 None:intergenic 50.0%
TTGGCGTGGTGTGCAAGAAT+TGG - chr7.1:77656147-77656166 MS.gene022086:CDS 50.0%
! GTGTGTTTGGGCTTTAGCCA+TGG - chr7.1:77656362-77656381 MS.gene022086:CDS 50.0%
! GTTTGGGCTTTAGCCATGGT+TGG - chr7.1:77656366-77656385 MS.gene022086:CDS 50.0%
AGCATCTTCCTGAAGGGACG+GGG + chr7.1:77657127-77657146 None:intergenic 55.0%
CCAGCCCAAAGCTATGGTGA+AGG - chr7.1:77656773-77656792 MS.gene022086:CDS 55.0%
CGCCTCTGCTTTCTCCAAGA+TGG - chr7.1:77657003-77657022 MS.gene022086:CDS 55.0%
GATCTTTACCCCGTCCCTTC+AGG - chr7.1:77657116-77657135 MS.gene022086:CDS 55.0%
!! CCTTCACCATAGCTTTGGGC+TGG + chr7.1:77656776-77656795 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 77656059 77657183 77656059 ID=MS.gene022086
chr7.1 mRNA 77656059 77657183 77656059 ID=MS.gene022086.t1;Parent=MS.gene022086
chr7.1 exon 77656059 77657183 77656059 ID=MS.gene022086.t1.exon1;Parent=MS.gene022086.t1
chr7.1 CDS 77656059 77657183 77656059 ID=cds.MS.gene022086.t1;Parent=MS.gene022086.t1
Gene Sequence

>MS.gene022086

ATGGGGAAGTTAGATTGGGCTAATTTAGAAGCCTATCCTCTTAGTTTGGTACTTGATAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGCGTGGTGTGCAAGAATTGGCTATCAATTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAATGTCCCACCTATGTTGATGATCCTCTCATACATAAATTATCGTATGCTTATAATATCACAGACGAAGAAGAGATATTTGTATAGTATTCTTAGTGAGAAAGAGTACCCTATTCAGTTATCCCATCCTTGCATCAAGAACATGAGTGTGTGTTTGGGCTTTAGCCATGGTTGGCTTGCATTAAAATATGATAACAAAGCCATAACACTAGTGAAACTTTTTAAGTATAGTGTAGCTCCCATTAGTTTGCCACCTCTCGAAAATATCAAGAAGGTTACCTTGTCTGCTGACCCGATTACAAGCCCAAGTGAGTATGTGGTTGCAGCGATTTACAATTGTGGTCGTCTCGCTTTGAAAAGAGCAAGCCAATCATTTTGGATCCGTGTAGACATAAATGAATTGTGGTGCACTAATGTTGTATTTTATAAAGGTCTGGTATTTGCTGACAACCTTGGACGCACCATTGTATCCTTTAAGTTCAATAATCCTCCTTGTGATGACTCAAATGACCCTAATTTTACTTGCTATGAGAAGATAGCTAGCACTAGATATAGTGTTCCAGCCCAAAGCTATGGTGAAGGTCGTTATTTTGTGAAATCGTTGAATGGAGACATATGGATGGTGAGAAGAAATTTAATCGATCAGTATGACAAGTCCTCGTATAATTTTGATGTTTACAAATTAAAACTGGATGCCCAGAGTGGAAAACTTGAACAAATGAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAACTTTGGTGATTCAATTTCTTTGTCCGCCTCTGCTTTCTCCAAGATGGAAAAGAATTCAATTTATTTTGTTGATGAAAGTGATAAAGATGGTATTGAGTTACATATCTATAATGCGGAGGATGGAAGTGTTGACAGTCGATCTTTACCCCGTCCCTTCAGGAAGATGCTACATTTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene022086.t1

MGKLDWANLEAYPLSLVLDKLEEHIDHVRFGVVCKNWLSIAKLNHKNHQFRINVPPMLMILSYINYRMLIISQTKKRYLYSILSEKEYPIQLSHPCIKNMSVCLGFSHGWLALKYDNKAITLVKLFKYSVAPISLPPLENIKKVTLSADPITSPSEYVVAAIYNCGRLALKRASQSFWIRVDINELWCTNVVFYKGLVFADNLGRTIVSFKFNNPPCDDSNDPNFTCYEKIASTRYSVPAQSYGEGRYFVKSLNGDIWMVRRNLIDQYDKSSYNFDVYKLKLDAQSGKLEQMNKLESLEDNILFVGNFGDSISLSASAFSKMEKNSIYFVDESDKDGIELHIYNAEDGSVDSRSLPRPFRKMLHFWVLPQFQWD