AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027042


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027042.t1 MTR_1g064570 65.056 269 76 7 1 255 1 265 2.02e-113 332
MS.gene027042.t1 MTR_5g038260 50.379 264 112 9 1 255 3 256 1.01e-76 237
MS.gene027042.t1 MTR_5g038230 46.899 258 104 10 1 255 1 228 3.54e-64 204
MS.gene027042.t1 MTR_5g082770 47.308 260 112 9 1 255 1 240 1.13e-63 201
MS.gene027042.t1 MTR_1g108740 43.396 265 115 9 4 255 5 247 1.29e-55 182
MS.gene027042.t1 MTR_7g077650 39.929 283 119 12 2 255 3 263 1.26e-46 159
MS.gene027042.t1 MTR_7g077620 38.043 276 130 9 2 255 3 259 2.36e-46 159
MS.gene027042.t1 MTR_7g077660 39.416 274 124 12 4 255 5 258 1.66e-44 154
MS.gene027042.t1 MTR_1g016150 40.670 209 93 6 4 200 5 194 7.45e-38 133
MS.gene027042.t1 MTR_6g023955 70.652 92 25 1 134 225 63 152 3.31e-37 130
MS.gene027042.t1 MTR_1g108750 37.805 164 59 4 1 162 1 123 2.73e-24 94.4
MS.gene027042.t1 MTR_5g082780 40.411 146 75 5 113 255 7 143 7.01e-23 94.0
MS.gene027042.t1 MTR_7g076110 37.356 174 85 7 94 255 38 199 6.81e-20 87.4
MS.gene027042.t1 MTR_3g114970 26.636 214 133 8 1 208 1 196 1.59e-14 72.8
MS.gene027042.t1 MTR_7g076100 37.500 120 62 3 145 254 69 185 2.59e-12 65.9
MS.gene027042.t1 MTR_7g076100 55.102 49 22 0 4 52 6 54 5.41e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCACTTTACATTCATAAT+TGG 0.230509 3.3:-43633062 None:intergenic
TAAAATCTGGACAAAACTTT+TGG 0.235612 3.3:+43633179 MS.gene027042:CDS
TTGCAACTTATAAATTGTTT+TGG 0.271302 3.3:-43632873 None:intergenic
CTTTGTTCTACACTTTCTTT+TGG 0.271432 3.3:-43632828 None:intergenic
TCTACATGTCGGATATAATT+AGG 0.288650 3.3:+43633032 MS.gene027042:CDS
CCACAAAACCTCTTGTTATA+AGG 0.323478 3.3:-43632905 None:intergenic
TATAATTGTATTTGTAACTT+TGG 0.337833 3.3:-43632963 None:intergenic
AGAACAAAGAGAAGGTTATA+TGG 0.364426 3.3:+43632842 MS.gene027042:CDS
ACCGATGTTATATTCTATAA+AGG 0.369663 3.3:+43633232 MS.gene027042:CDS
CAAAGGTCAATTATCAAAAT+CGG 0.402117 3.3:+43632765 MS.gene027042:CDS
GGCTTATCTTGTGAAATCAT+TGG 0.406267 3.3:+43633372 MS.gene027042:CDS
CATTCCTAATGTTGTTTCTC+CGG 0.418552 3.3:+43633327 MS.gene027042:CDS
TCTGCTAAATCCTTTCAAAC+AGG 0.421433 3.3:+43632988 MS.gene027042:CDS
TCAGCGTGGTATGCAAGAAT+TGG 0.424757 3.3:+43632732 MS.gene027042:CDS
TAATCATGTGGCCTAGTTGT+AGG 0.431478 3.3:-43633106 None:intergenic
TAGTCTATGCTGTGGGTTGG+TGG 0.434022 3.3:+43633257 MS.gene027042:CDS
ATTATATCCGACATGTAGAT+AGG 0.442904 3.3:-43633028 None:intergenic
CTTTGCTAGCCAACCATGAC+TGG 0.448989 3.3:-43632930 None:intergenic
TGTCTCGCTTTCATAAAATC+TGG 0.450132 3.3:+43633166 MS.gene027042:CDS
TCTTCTCAATGGAGGCAGAT+TGG 0.454302 3.3:+43632639 None:intergenic
TTATGAATGTAAAGTGCATA+AGG 0.479221 3.3:+43633066 MS.gene027042:CDS
GAACAAAGAGAAGGTTATAT+GGG 0.483307 3.3:+43632843 MS.gene027042:CDS
TAATGTTGTTTCTCCGGAGG+GGG 0.487050 3.3:+43633333 MS.gene027042:CDS
ACATGTAGATAGGTGGTAAA+GGG 0.494359 3.3:-43633018 None:intergenic
CCCTCCGGAGAAACAACATT+AGG 0.500061 3.3:-43633331 None:intergenic
TTGATAATTGACCTTTGCAA+TGG 0.519800 3.3:-43632759 None:intergenic
GACATGTAGATAGGTGGTAA+AGG 0.520441 3.3:-43633019 None:intergenic
GGGATGGAAACCTGTTTGAA+AGG 0.525110 3.3:-43632998 None:intergenic
CCTAATGTTGTTTCTCCGGA+GGG 0.527562 3.3:+43633331 MS.gene027042:CDS
TTTGTGGCTCCAGTCATGGT+TGG 0.527925 3.3:+43632921 MS.gene027042:CDS
GCCTAGTCTATGCTGTGGGT+TGG 0.537826 3.3:+43633254 MS.gene027042:CDS
TTATCTTGTGAAATCATTGG+AGG 0.548164 3.3:+43633375 MS.gene027042:CDS
TCCTAATGTTGTTTCTCCGG+AGG 0.554077 3.3:+43633330 MS.gene027042:CDS
TTTGGTGGGAATCATTAGCA+TGG 0.559241 3.3:-43632810 None:intergenic
GAAAGTGTAGAACAAAGAGA+AGG 0.562266 3.3:+43632834 MS.gene027042:CDS
CTAATGTTGTTTCTCCGGAG+GGG 0.563359 3.3:+43633332 MS.gene027042:CDS
CAGTCATGGTTGGCTAGCAA+AGG 0.570941 3.3:+43632931 MS.gene027042:CDS
GTAGATAGGTGGTAAAGGGA+TGG 0.572882 3.3:-43633014 None:intergenic
TATCGGCTAATCCTACAACT+AGG 0.572975 3.3:+43633095 MS.gene027042:CDS
TTGATGTGCCTTATAACAAG+AGG 0.579292 3.3:+43632897 MS.gene027042:CDS
TTGGTGGGAATCATTAGCAT+GGG 0.584462 3.3:-43632809 None:intergenic
GTGTTACTCGAAGGATGAGA+AGG 0.591492 3.3:+43633303 MS.gene027042:CDS
TATCTTGTGAAATCATTGGA+GGG 0.603454 3.3:+43633376 MS.gene027042:CDS
TCATGGTTGGCTAGCAAAGG+CGG 0.620668 3.3:+43632934 MS.gene027042:CDS
GAATTGGTATTCCATTGCAA+AGG 0.622427 3.3:+43632748 MS.gene027042:CDS
AAAGGCCTAGTCTATGCTGT+GGG 0.634832 3.3:+43633250 MS.gene027042:CDS
AATCATTGGAGGGAGACTTG+TGG 0.640759 3.3:+43633386 MS.gene027042:CDS
TAAAGGCCTAGTCTATGCTG+TGG 0.643845 3.3:+43633249 MS.gene027042:CDS
TTTACCACCTATCTACATGT+CGG 0.651760 3.3:+43633021 MS.gene027042:CDS
ACCAACCCACAGCATAGACT+AGG 0.655708 3.3:-43633255 None:intergenic
ATATCCGACATGTAGATAGG+TGG 0.675448 3.3:-43633025 None:intergenic
GCCTTTATAGAATATAACAT+CGG 0.679693 3.3:-43633233 None:intergenic
GCTACAGCAACATAATCATG+TGG 0.697559 3.3:-43633118 None:intergenic
AGATCACATATATTTCAGCG+TGG 0.757762 3.3:+43632718 MS.gene027042:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTGTATTTGTAACTT+TGG - chr3.3:43632966-43632985 None:intergenic 15.0%
!! GGAATTTATAATGTAAAAGA+TGG + chr3.3:43633775-43633794 MS.gene027042:intron 20.0%
!! TTATAAATTCCCATATCAAA+CGG - chr3.3:43633766-43633785 None:intergenic 20.0%
!!! AATGTTAAAACTTGATAGTT+TGG + chr3.3:43633603-43633622 MS.gene027042:intron 20.0%
!!! ATGTTAAAACTTGATAGTTT+GGG + chr3.3:43633604-43633623 MS.gene027042:intron 20.0%
!!! TTGCAACTTATAAATTGTTT+TGG - chr3.3:43632876-43632895 None:intergenic 20.0%
! ACCGATGTTATATTCTATAA+AGG + chr3.3:43633232-43633251 MS.gene027042:CDS 25.0%
! ATGCACTTTACATTCATAAT+TGG - chr3.3:43633065-43633084 None:intergenic 25.0%
! CAAAGGTCAATTATCAAAAT+CGG + chr3.3:43632765-43632784 MS.gene027042:CDS 25.0%
! GCCTTTATAGAATATAACAT+CGG - chr3.3:43633236-43633255 None:intergenic 25.0%
! TTATGAATGTAAAGTGCATA+AGG + chr3.3:43633066-43633085 MS.gene027042:CDS 25.0%
!! TAAAATCTGGACAAAACTTT+TGG + chr3.3:43633179-43633198 MS.gene027042:CDS 25.0%
!!! AAGTTTGATTTTTGACAAGT+TGG + chr3.3:43632685-43632704 MS.gene027042:CDS 25.0%
!!! AGTGCATAAGGTTATTTTAT+CGG + chr3.3:43633078-43633097 MS.gene027042:CDS 25.0%
!!! TTTTAATTTGTGTTACTCGA+AGG + chr3.3:43633294-43633313 MS.gene027042:CDS 25.0%
AGAACAAAGAGAAGGTTATA+TGG + chr3.3:43632842-43632861 MS.gene027042:CDS 30.0%
ATTATATCCGACATGTAGAT+AGG - chr3.3:43633031-43633050 None:intergenic 30.0%
CAATGAATCTGTATCTATGT+CGG + chr3.3:43633651-43633670 MS.gene027042:intron 30.0%
GAACAAAGAGAAGGTTATAT+GGG + chr3.3:43632843-43632862 MS.gene027042:CDS 30.0%
TATCTTGTGAAATCATTGGA+GGG + chr3.3:43633376-43633395 MS.gene027042:CDS 30.0%
TCTACATGTCGGATATAATT+AGG + chr3.3:43633032-43633051 MS.gene027042:CDS 30.0%
TTATCTTGTGAAATCATTGG+AGG + chr3.3:43633375-43633394 MS.gene027042:CDS 30.0%
TTGGAATTAGATCTTCAAAG+TGG + chr3.3:43633568-43633587 MS.gene027042:intron 30.0%
! CATATTTGAAGTCTACAAGT+TGG + chr3.3:43633549-43633568 MS.gene027042:intron 30.0%
! CTTTGTTCTACACTTTCTTT+TGG - chr3.3:43632831-43632850 None:intergenic 30.0%
! TGGCTTGTAAGAAAATATGT+TGG + chr3.3:43633406-43633425 MS.gene027042:intron 30.0%
! TTGATAATTGACCTTTGCAA+TGG - chr3.3:43632762-43632781 None:intergenic 30.0%
AATATGCTAGTACCACTACT+AGG - chr3.3:43633535-43633554 None:intergenic 35.0%
ACATGTAGATAGGTGGTAAA+GGG - chr3.3:43633021-43633040 None:intergenic 35.0%
AGATCACATATATTTCAGCG+TGG + chr3.3:43632718-43632737 MS.gene027042:CDS 35.0%
GAATTGGTATTCCATTGCAA+AGG + chr3.3:43632748-43632767 MS.gene027042:CDS 35.0%
GGCTTATCTTGTGAAATCAT+TGG + chr3.3:43633372-43633391 MS.gene027042:CDS 35.0%
TCTGCTAAATCCTTTCAAAC+AGG + chr3.3:43632988-43633007 MS.gene027042:CDS 35.0%
TGTCTCGCTTTCATAAAATC+TGG + chr3.3:43633166-43633185 MS.gene027042:CDS 35.0%
TTGATGTGCCTTATAACAAG+AGG + chr3.3:43632897-43632916 MS.gene027042:CDS 35.0%
TTTACCACCTATCTACATGT+CGG + chr3.3:43633021-43633040 MS.gene027042:CDS 35.0%
! AGATTTTATGAAAGCGAGAC+AGG - chr3.3:43633167-43633186 None:intergenic 35.0%
! CCACAAAACCTCTTGTTATA+AGG - chr3.3:43632908-43632927 None:intergenic 35.0%
! CCTTATAACAAGAGGTTTTG+TGG + chr3.3:43632905-43632924 MS.gene027042:CDS 35.0%
! GAAAGTGTAGAACAAAGAGA+AGG + chr3.3:43632834-43632853 MS.gene027042:CDS 35.0%
! GATGATGATGATGATGATGA+TGG + chr3.3:43633487-43633506 MS.gene027042:intron 35.0%
! GTTCTACACTTTCTTTTGGT+GGG - chr3.3:43632827-43632846 None:intergenic 35.0%
! TGTTCTACACTTTCTTTTGG+TGG - chr3.3:43632828-43632847 None:intergenic 35.0%
! TTTCTCGATGATGATGATGA+TGG + chr3.3:43633430-43633449 MS.gene027042:intron 35.0%
!! CATTCCTAATGTTGTTTCTC+CGG + chr3.3:43633327-43633346 MS.gene027042:CDS 35.0%
!!! GGAGACAACGTTTTATTTGT+GGG + chr3.3:43633625-43633644 MS.gene027042:intron 35.0%
ATATCCGACATGTAGATAGG+TGG - chr3.3:43633028-43633047 None:intergenic 40.0%
CCATATCAAACGGTCCATTA+GGG - chr3.3:43633756-43633775 None:intergenic 40.0%
GACATGTAGATAGGTGGTAA+AGG - chr3.3:43633022-43633041 None:intergenic 40.0%
GCTACAGCAACATAATCATG+TGG - chr3.3:43633121-43633140 None:intergenic 40.0%
GGAGGCAGATTGGAAAAATT+TGG + chr3.3:43632649-43632668 MS.gene027042:CDS 40.0%
GTCAACATTGCCGTTATCAT+TGG + chr3.3:43633806-43633825 MS.gene027042:intron 40.0%
TAATCATGTGGCCTAGTTGT+AGG - chr3.3:43633109-43633128 None:intergenic 40.0%
TCCGTGTGAACCAATGATAA+CGG - chr3.3:43633819-43633838 None:intergenic 40.0%
TTGGTGGGAATCATTAGCAT+GGG - chr3.3:43632812-43632831 None:intergenic 40.0%
TTTGGTGGGAATCATTAGCA+TGG - chr3.3:43632813-43632832 None:intergenic 40.0%
! CCTAATGGACCGTTTGATAT+GGG + chr3.3:43633754-43633773 MS.gene027042:intron 40.0%
! TATCGGCTAATCCTACAACT+AGG + chr3.3:43633095-43633114 MS.gene027042:CDS 40.0%
!! GATGATGATGGTGATGATGA+TGG + chr3.3:43633442-43633461 MS.gene027042:intron 40.0%
!!! GGGAGACAACGTTTTATTTG+TGG + chr3.3:43633624-43633643 MS.gene027042:intron 40.0%
AAAGGCCTAGTCTATGCTGT+GGG + chr3.3:43633250-43633269 MS.gene027042:CDS 45.0%
AATCATTGGAGGGAGACTTG+TGG + chr3.3:43633386-43633405 MS.gene027042:CDS 45.0%
AGGGGGATGATTATGCTCAT+CGG + chr3.3:43633350-43633369 MS.gene027042:CDS 45.0%
CCCATATCAAACGGTCCATT+AGG - chr3.3:43633757-43633776 None:intergenic 45.0%
GGGATGGAAACCTGTTTGAA+AGG - chr3.3:43633001-43633020 None:intergenic 45.0%
GGTCCATTAGGGTAAGGAAT+CGG - chr3.3:43633745-43633764 None:intergenic 45.0%
GTAGATAGGTGGTAAAGGGA+TGG - chr3.3:43633017-43633036 None:intergenic 45.0%
TAAAGGCCTAGTCTATGCTG+TGG + chr3.3:43633249-43633268 MS.gene027042:CDS 45.0%
TCAAACGGTCCATTAGGGTA+AGG - chr3.3:43633751-43633770 None:intergenic 45.0%
TCAGCGTGGTATGCAAGAAT+TGG + chr3.3:43632732-43632751 MS.gene027042:CDS 45.0%
! CCCTAATGGACCGTTTGATA+TGG + chr3.3:43633753-43633772 MS.gene027042:intron 45.0%
! GGTGGTGATGATGATGATGA+TGG + chr3.3:43633463-43633482 MS.gene027042:intron 45.0%
! GTGTTACTCGAAGGATGAGA+AGG + chr3.3:43633303-43633322 MS.gene027042:CDS 45.0%
!! CCTAATGTTGTTTCTCCGGA+GGG + chr3.3:43633331-43633350 MS.gene027042:CDS 45.0%
!! CTAATGTTGTTTCTCCGGAG+GGG + chr3.3:43633332-43633351 MS.gene027042:CDS 45.0%
!! GATGATGGTGATGATGATGG+TGG + chr3.3:43633445-43633464 MS.gene027042:intron 45.0%
!! GCCGTTATCATTGGTTCACA+CGG + chr3.3:43633815-43633834 MS.gene027042:intron 45.0%
!! GGTGTTAACTTGCCTAGTAG+TGG + chr3.3:43633520-43633539 MS.gene027042:intron 45.0%
!! TAATGTTGTTTCTCCGGAGG+GGG + chr3.3:43633333-43633352 MS.gene027042:CDS 45.0%
!! TCCTAATGTTGTTTCTCCGG+AGG + chr3.3:43633330-43633349 MS.gene027042:CDS 45.0%
ACCAACCCACAGCATAGACT+AGG - chr3.3:43633258-43633277 None:intergenic 50.0%
CCCTCCGGAGAAACAACATT+AGG - chr3.3:43633334-43633353 None:intergenic 50.0%
CTTTGCTAGCCAACCATGAC+TGG - chr3.3:43632933-43632952 None:intergenic 50.0%
GGGGGATGATTATGCTCATC+GGG + chr3.3:43633351-43633370 MS.gene027042:CDS 50.0%
TGAGCATAATCATCCCCCTC+CGG - chr3.3:43633349-43633368 None:intergenic 50.0%
TTTGTGGCTCCAGTCATGGT+TGG + chr3.3:43632921-43632940 MS.gene027042:CDS 50.0%
! CAGTCATGGTTGGCTAGCAA+AGG + chr3.3:43632931-43632950 MS.gene027042:CDS 50.0%
! GGGTTCTTCCACCTTTTCAG+TGG + chr3.3:43633854-43633873 MS.gene027042:intron 50.0%
! TCATGGTTGGCTAGCAAAGG+CGG + chr3.3:43632934-43632953 MS.gene027042:CDS 50.0%
!! AGGTTTTGTGGCTCCAGTCA+TGG + chr3.3:43632917-43632936 MS.gene027042:CDS 50.0%
!! GCACCGATTCCTTACCCTAA+TGG + chr3.3:43633739-43633758 MS.gene027042:intron 50.0%
!! TAGTCTATGCTGTGGGTTGG+TGG + chr3.3:43633257-43633276 MS.gene027042:CDS 50.0%
ACGGATGCCACCTGCACTAT+GGG + chr3.3:43633834-43633853 MS.gene027042:intron 55.0%
GCCTAGTCTATGCTGTGGGT+TGG + chr3.3:43633254-43633273 MS.gene027042:CDS 55.0%
GGAAGAACCCATAGTGCAGG+TGG - chr3.3:43633844-43633863 None:intergenic 55.0%
GGTGGAAGAACCCATAGTGC+AGG - chr3.3:43633847-43633866 None:intergenic 55.0%
CACGGATGCCACCTGCACTA+TGG + chr3.3:43633833-43633852 MS.gene027042:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 43632647 43633882 43632647 ID=MS.gene027042
chr3.3 mRNA 43632647 43633882 43632647 ID=MS.gene027042.t1;Parent=MS.gene027042
chr3.3 exon 43632647 43633411 43632647 ID=MS.gene027042.t1.exon1;Parent=MS.gene027042.t1
chr3.3 CDS 43632647 43633411 43632647 ID=cds.MS.gene027042.t1;Parent=MS.gene027042.t1
chr3.3 exon 43633874 43633882 43633874 ID=MS.gene027042.t1.exon2;Parent=MS.gene027042.t1
chr3.3 CDS 43633874 43633882 43633874 ID=cds.MS.gene027042.t1;Parent=MS.gene027042.t1
Gene Sequence

>MS.gene027042

ATGGAGGCAGATTGGAAAAATTTGGAAGCACTTTCTCTAAGTTTGATTTTTGACAAGTTGGTAGAGCGTATAGATCACATATATTTCAGCGTGGTATGCAAGAATTGGTATTCCATTGCAAAGGTCAATTATCAAAATCGGCAAATCGAAAATAATATACTGCCCATGCTAATGATTCCCACCAAAAGAAAGTGTAGAACAAAGAGAAGGTTATATGGGATTTCATCCAAAACAATTTATAAGTTGCAACTTGATGTGCCTTATAACAAGAGGTTTTGTGGCTCCAGTCATGGTTGGCTAGCAAAGGCGGAGTATTCCAAAGTTACAAATACAATTATAACTCTGCTAAATCCTTTCAAACAGGTTTCCATCCCTTTACCACCTATCTACATGTCGGATATAATTAGGAGAAGAACCAATTATGAATGTAAAGTGCATAAGGTTATTTTATCGGCTAATCCTACAACTAGGCCACATGATTATGTTGCTGTAGCAATCTATAGCATGCGTACGCGTACCTGTCTCGCTTTCATAAAATCTGGACAAAACTTTTGGACTTTTGTTGATTACGACTATTACTGCTATACCGATGTTATATTCTATAAAGGCCTAGTCTATGCTGTGGGTTGGTGGAATAACATTGTCTCTTTTAATTTGTGTTACTCGAAGGATGAGAAGGTCATTCCTAATGTTGTTTCTCCGGAGGGGGATGATTATGCTCATCGGGCTTATCTTGTGAAATCATTGGAGGGAGACTTGTGGCTTGTAAGAAAATATGTTGGTTTTCTCGATGATGATGATGATGGTGATGATGATGGTGGTGATGATGATGATGATGGTGATGATGATGATGATGATGATGATGATGATGATGGTGTTAACTTGCCTAGTAGTGGTACTAGCATATTTGAAGTCTACAAGTTGGAATTAGATCTTCAAAGTGGTAAGCTTATACAAATGTTAAAACTTGATAGTTTGGGAGACAACGTTTTATTTGTGGGCGACAATGAATCTGTATCTATGTCGGCTTCATACTTCTCTAATTATCTACAAAAAGATTCAATCTATTATACTGATGACTTTTATGAAGACGCACCGATTCCTTACCCTAATGGACCGTTTGATATGGGAATTTATAATGTAAAAGATGGAAGCTTTAGTCAACATTGCCGTTATCATTGGTTCACACGGATGCCACCTGCACTATGGGTTCTTCCACCTTTTCAGTGGAATTGA

Protein sequence

>MS.gene027042.t1

MEADWKNLEALSLSLIFDKLVERIDHIYFSVVCKNWYSIAKVNYQNRQIENNILPMLMIPTKRKCRTKRRLYGISSKTIYKLQLDVPYNKRFCGSSHGWLAKAEYSKVTNTIITLLNPFKQVSIPLPPIYMSDIIRRRTNYECKVHKVILSANPTTRPHDYVAVAIYSMRTRTCLAFIKSGQNFWTFVDYDYYCYTDVIFYKGLVYAVGWWNNIVSFNLCYSKDEKVIPNVVSPEGDDYAHRAYLVKSLEGDLWLWN