AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76061


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76061.t1 MTR_5g082780 90.909 231 21 0 98 328 1 231 2.74e-153 431
MS.gene76061.t1 MTR_5g082770 63.605 294 93 5 1 284 1 290 1.03e-119 348
MS.gene76061.t1 MTR_5g038260 45.953 383 179 10 1 359 3 381 2.10e-103 310
MS.gene76061.t1 MTR_1g064570 43.781 402 179 10 1 359 1 398 7.85e-101 303
MS.gene76061.t1 MTR_5g038230 46.257 374 165 10 1 359 1 353 2.78e-99 298
MS.gene76061.t1 MTR_1g108740 42.553 376 160 11 4 359 5 344 4.36e-84 259
MS.gene76061.t1 MTR_7g077620 41.162 396 167 14 2 359 3 370 6.98e-75 236
MS.gene76061.t1 MTR_7g077660 41.878 394 161 18 3 359 4 366 1.72e-73 232
MS.gene76061.t1 MTR_7g077650 40.299 402 160 17 2 358 3 369 9.95e-71 225
MS.gene76061.t1 MTR_1g016150 45.405 185 74 5 4 171 5 179 1.10e-40 143
MS.gene76061.t1 MTR_1g016150 47.436 78 32 1 219 296 168 236 4.68e-13 68.6
MS.gene76061.t1 MTR_7g076110 35.144 313 154 13 73 359 22 311 2.37e-36 134
MS.gene76061.t1 MTR_7g076100 39.676 247 111 10 133 359 70 298 2.92e-36 133
MS.gene76061.t1 MTR_1g108750 45.695 151 54 3 1 150 1 124 1.64e-27 105
MS.gene76061.t1 MTR_3g114990 25.980 408 232 16 1 357 20 408 1.86e-20 92.0
MS.gene76061.t1 MTR_3g114970 26.854 391 214 23 1 357 1 353 1.83e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATCTCATTGGAAATT+TGG 0.118149 5.2:+70761639 None:intergenic
GGAACTAGATTGGGCAAATT+TGG 0.170985 5.2:-70762702 MS.gene76061:CDS
TAGCATTTGTTTAAAATTTC+CGG 0.173834 5.2:+70761920 None:intergenic
TAAGAATTTGGCAATTTAAA+TGG 0.183830 5.2:+70762479 None:intergenic
AGCGGCAGCCACCACCTTTC+TGG 0.185311 5.2:-70761668 MS.gene76061:CDS
TAATCATTTGGTCTTGTTAT+AGG 0.187600 5.2:+70762284 None:intergenic
TACAAACATCTTTCTGTTCT+TGG 0.199967 5.2:+70762527 None:intergenic
GAGCTGGAGTCATTCCTTTA+TGG 0.214315 5.2:-70762174 MS.gene76061:CDS
TTTATGGATGTTATGTTCTA+TGG 0.218295 5.2:-70762158 MS.gene76061:CDS
CAAATATGGTAATAAGAATT+TGG 0.251801 5.2:+70762467 None:intergenic
CAAATAGATCACATTTGTTT+TGG 0.278174 5.2:-70762638 MS.gene76061:CDS
GGCTAAATCCACAACAAATA+TGG 0.289458 5.2:+70762453 None:intergenic
AATCATTTGGTCTTGTTATA+GGG 0.294407 5.2:+70762285 None:intergenic
AACAAGACCAAATGATTATA+TGG 0.314220 5.2:-70762279 MS.gene76061:CDS
ACTTGTGGTCCATTTCATAT+AGG 0.341950 5.2:-70761753 MS.gene76061:CDS
GCTATGTCCTGAATTGGATT+TGG 0.346401 5.2:+70762065 None:intergenic
TTCTAAACCGCTGATCTTGA+TGG 0.357058 5.2:+70762576 None:intergenic
TGAAAAGCTATGTCCTGAAT+TGG 0.358033 5.2:+70762059 None:intergenic
GCGGCAGCCACCACCTTTCT+GGG 0.359418 5.2:-70761667 MS.gene76061:CDS
GATAACTCCAAATCCAATTC+AGG 0.366133 5.2:-70762072 MS.gene76061:CDS
TCGAGGGTTGGAAGACTAAT+AGG 0.372920 5.2:+70762362 None:intergenic
ACAAACATCTTTCTGTTCTT+GGG 0.381418 5.2:+70762528 None:intergenic
GCCGCAACCATATAATCATT+TGG 0.404338 5.2:+70762272 None:intergenic
GCAATTCACACTAGCATTCA+TGG 0.409604 5.2:-70762251 MS.gene76061:CDS
TGGTTAGAATGGAACTAGAT+TGG 0.415806 5.2:-70762712 None:intergenic
TGATTTAATTCTTGATAAGT+TGG 0.416195 5.2:-70762666 MS.gene76061:CDS
CTTGTGGTCCATTTCATATA+GGG 0.417934 5.2:-70761752 MS.gene76061:CDS
GGTTAGAATGGAACTAGATT+GGG 0.421888 5.2:-70762711 None:intergenic
TTATATATCCCTATATGAAA+TGG 0.424795 5.2:+70761744 None:intergenic
TTATCTTGTGAAATCATTAG+AGG 0.436221 5.2:-70762021 MS.gene76061:CDS
ATAGGAGCAACATTCTTAGA+AGG 0.444870 5.2:+70762380 None:intergenic
TGAATTCCAACTTGTACACT+TGG 0.447974 5.2:+70761952 None:intergenic
TTGGCGCCGTGTGCAAGAAT+TGG 0.448573 5.2:-70762619 MS.gene76061:CDS
ATAGAACATAACATCCATAA+AGG 0.458967 5.2:+70762160 None:intergenic
AGAAGGATGCAATAGTGTTA+TGG 0.460688 5.2:+70762397 None:intergenic
TGTTCTTGGGATCATTAGCA+TGG 0.462460 5.2:+70762541 None:intergenic
ATGCTAAAGCTTGAGAGTCT+TGG 0.465110 5.2:-70761903 MS.gene76061:CDS
TAAAAGCGGGCGAACCATGC+TGG 0.470666 5.2:-70762214 MS.gene76061:CDS
GTATCAGCTTCATACTTCTC+CGG 0.473456 5.2:-70761840 MS.gene76061:CDS
AACCATGCTGGACTTATGTA+TGG 0.481402 5.2:-70762202 MS.gene76061:CDS
TCTTATTACCATATTTGTTG+TGG 0.483010 5.2:-70762461 MS.gene76061:CDS
CTTATGTATGGAGCTTGAGC+TGG 0.493140 5.2:-70762190 MS.gene76061:CDS
TTTATCTGAGGTCGGGTACA+AGG 0.517119 5.2:-70762324 MS.gene76061:CDS
ACCAAATGATTATATGGTTG+CGG 0.517425 5.2:-70762273 MS.gene76061:CDS
TGATTTCCAAGTGTACAAGT+TGG 0.520656 5.2:-70761958 MS.gene76061:CDS
GTTGTGGATTTAGCCATGGT+TGG 0.523421 5.2:-70762445 MS.gene76061:CDS
GTGAAGATTATGATGATCAA+TGG 0.528142 5.2:+70761707 None:intergenic
ATACAAGTTTATCTGAGGTC+GGG 0.533775 5.2:-70762331 MS.gene76061:CDS
GGGATATATAATGTAAGAGA+TGG 0.535003 5.2:-70761732 MS.gene76061:CDS
TATACAAGTTTATCTGAGGT+CGG 0.543905 5.2:-70762332 MS.gene76061:CDS
ATTTGTTGTGGATTTAGCCA+TGG 0.546254 5.2:-70762449 MS.gene76061:CDS
ATGGATGTTATGTTCTATGG+AGG 0.548376 5.2:-70762155 MS.gene76061:CDS
AAATTTGGTAAAACCCAGAA+AGG 0.548520 5.2:+70761654 None:intergenic
GTTCTTGGGATCATTAGCAT+GGG 0.554796 5.2:+70762542 None:intergenic
TTGGAATTCAATGCTCAGAC+CGG 0.564684 5.2:-70761939 MS.gene76061:CDS
TATCTTGTGAAATCATTAGA+GGG 0.568311 5.2:-70762020 MS.gene76061:CDS
GGTAAAACCCAGAAAGGTGG+TGG 0.590899 5.2:+70761660 None:intergenic
ACAATCATTTCTTCAACAAG+CGG 0.597253 5.2:-70761686 MS.gene76061:CDS
TATGTTGCTAGTAAATATCA+CGG 0.603958 5.2:-70762125 MS.gene76061:CDS
CTATTGTTGCAAGCCAACCA+TGG 0.611822 5.2:+70762432 None:intergenic
AACTTGTATATGATCGATCG+AGG 0.611957 5.2:+70762345 None:intergenic
AAGTTAGATTGTGTAACTTG+TGG 0.613074 5.2:-70761768 MS.gene76061:CDS
ATCATATACAAGTTTATCTG+AGG 0.615631 5.2:-70762336 MS.gene76061:CDS
GTATATGATCGATCGAGGGT+TGG 0.616455 5.2:+70762350 None:intergenic
AGCTTAACCATCAAGATCAG+CGG 0.626714 5.2:-70762583 MS.gene76061:CDS
CTCCATACATAAGTCCAGCA+TGG 0.636983 5.2:+70762200 None:intergenic
AATCATTAGAGGGAGACTTG+TGG 0.640892 5.2:-70762010 MS.gene76061:CDS
TTTGGTAAAACCCAGAAAGG+TGG 0.643098 5.2:+70761657 None:intergenic
TAATCGCCAATTCTTGCACA+CGG 0.653587 5.2:+70762613 None:intergenic
ACTTGTATATGATCGATCGA+GGG 0.674359 5.2:+70762346 None:intergenic
ATTAGAGGGAGACTTGTGGA+TGG 0.690554 5.2:-70762006 MS.gene76061:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATGGATACATTTTTT+TGG + chr5.2:70761861-70761880 None:intergenic 15.0%
!! CAAATATGGTAATAAGAATT+TGG + chr5.2:70761887-70761906 None:intergenic 20.0%
!! TAAGAATTTGGCAATTTAAA+TGG + chr5.2:70761875-70761894 None:intergenic 20.0%
!! TGATTTAATTCTTGATAAGT+TGG - chr5.2:70761685-70761704 MS.gene76061:CDS 20.0%
!! TTATATATCCCTATATGAAA+TGG + chr5.2:70762610-70762629 None:intergenic 20.0%
!!! ATGTTTCGTTTTTATAAAAG+CGG - chr5.2:70762123-70762142 MS.gene76061:CDS 20.0%
!!! TAGCATTTGTTTAAAATTTC+CGG + chr5.2:70762434-70762453 None:intergenic 20.0%
! AACAAGACCAAATGATTATA+TGG - chr5.2:70762072-70762091 MS.gene76061:CDS 25.0%
! ATAGAACATAACATCCATAA+AGG + chr5.2:70762194-70762213 None:intergenic 25.0%
! ATCATATACAAGTTTATCTG+AGG - chr5.2:70762015-70762034 MS.gene76061:CDS 25.0%
! TATCTTGTGAAATCATTAGA+GGG - chr5.2:70762331-70762350 MS.gene76061:CDS 25.0%
! TATGTTGCTAGTAAATATCA+CGG - chr5.2:70762226-70762245 MS.gene76061:CDS 25.0%
! TTATCTTGTGAAATCATTAG+AGG - chr5.2:70762330-70762349 MS.gene76061:CDS 25.0%
! TTTATGGATGTTATGTTCTA+TGG - chr5.2:70762193-70762212 MS.gene76061:CDS 25.0%
!! CAAATAGATCACATTTGTTT+TGG - chr5.2:70761713-70761732 MS.gene76061:CDS 25.0%
!! GGTATAGTATCTTTTAATCT+TGG - chr5.2:70762247-70762266 MS.gene76061:CDS 25.0%
!! TCTTATTACCATATTTGTTG+TGG - chr5.2:70761890-70761909 MS.gene76061:CDS 25.0%
!!! AATCATTTGGTCTTGTTATA+GGG + chr5.2:70762069-70762088 None:intergenic 25.0%
!!! TAATCATTTGGTCTTGTTAT+AGG + chr5.2:70762070-70762089 None:intergenic 25.0%
!!! TGTTTCGTTTTTATAAAAGC+GGG - chr5.2:70762124-70762143 MS.gene76061:CDS 25.0%
AAATTTGGTAAAACCCAGAA+AGG + chr5.2:70762700-70762719 None:intergenic 30.0%
AAGTTAGATTGTGTAACTTG+TGG - chr5.2:70762583-70762602 MS.gene76061:CDS 30.0%
ACAAACATCTTTCTGTTCTT+GGG + chr5.2:70761826-70761845 None:intergenic 30.0%
ACAATCATTTCTTCAACAAG+CGG - chr5.2:70762665-70762684 MS.gene76061:CDS 30.0%
ACCAAATGATTATATGGTTG+CGG - chr5.2:70762078-70762097 MS.gene76061:CDS 30.0%
GGGATATATAATGTAAGAGA+TGG - chr5.2:70762619-70762638 MS.gene76061:CDS 30.0%
GTGAAGATTATGATGATCAA+TGG + chr5.2:70762647-70762666 None:intergenic 30.0%
TACAAACATCTTTCTGTTCT+TGG + chr5.2:70761827-70761846 None:intergenic 30.0%
! CTTTTAATCTTGGTCATAAG+AGG - chr5.2:70762257-70762276 MS.gene76061:CDS 30.0%
! TATACAAGTTTATCTGAGGT+CGG - chr5.2:70762019-70762038 MS.gene76061:CDS 30.0%
!!! TGAATCTTTTTTCAGACAAC+CGG + chr5.2:70762533-70762552 None:intergenic 30.0%
AACTTGTATATGATCGATCG+AGG + chr5.2:70762009-70762028 None:intergenic 35.0%
ACTTGTATATGATCGATCGA+GGG + chr5.2:70762008-70762027 None:intergenic 35.0%
ACTTGTGGTCCATTTCATAT+AGG - chr5.2:70762598-70762617 MS.gene76061:CDS 35.0%
AGAAGGATGCAATAGTGTTA+TGG + chr5.2:70761957-70761976 None:intergenic 35.0%
ATAGGAGCAACATTCTTAGA+AGG + chr5.2:70761974-70761993 None:intergenic 35.0%
ATGGATGTTATGTTCTATGG+AGG - chr5.2:70762196-70762215 MS.gene76061:CDS 35.0%
CTTGTGGTCCATTTCATATA+GGG - chr5.2:70762599-70762618 MS.gene76061:CDS 35.0%
GATAACTCCAAATCCAATTC+AGG - chr5.2:70762279-70762298 MS.gene76061:CDS 35.0%
GGCTAAATCCACAACAAATA+TGG + chr5.2:70761901-70761920 None:intergenic 35.0%
TGAAAAGCTATGTCCTGAAT+TGG + chr5.2:70762295-70762314 None:intergenic 35.0%
TGAATTCCAACTTGTACACT+TGG + chr5.2:70762402-70762421 None:intergenic 35.0%
TGATTTCCAAGTGTACAAGT+TGG - chr5.2:70762393-70762412 MS.gene76061:CDS 35.0%
! ATACAAGTTTATCTGAGGTC+GGG - chr5.2:70762020-70762039 MS.gene76061:CDS 35.0%
! ATTCAGGACATAGCTTTTCA+TGG - chr5.2:70762295-70762314 MS.gene76061:CDS 35.0%
! GCTTTTCATGGAAGGTTTAA+AGG - chr5.2:70762307-70762326 MS.gene76061:CDS 35.0%
!! ATTTGTTGTGGATTTAGCCA+TGG - chr5.2:70761902-70761921 MS.gene76061:CDS 35.0%
!! GGAGACAATGTTTTGTTCTT+AGG - chr5.2:70762469-70762488 MS.gene76061:CDS 35.0%
AACCATGCTGGACTTATGTA+TGG - chr5.2:70762149-70762168 MS.gene76061:CDS 40.0%
AATCATTAGAGGGAGACTTG+TGG - chr5.2:70762341-70762360 MS.gene76061:CDS 40.0%
AGCTTAACCATCAAGATCAG+CGG - chr5.2:70761768-70761787 MS.gene76061:CDS 40.0%
ATGCTAAAGCTTGAGAGTCT+TGG - chr5.2:70762448-70762467 MS.gene76061:CDS 40.0%
GCAATTCACACTAGCATTCA+TGG - chr5.2:70762100-70762119 MS.gene76061:CDS 40.0%
GCCGCAACCATATAATCATT+TGG + chr5.2:70762082-70762101 None:intergenic 40.0%
GCTATGTCCTGAATTGGATT+TGG + chr5.2:70762289-70762308 None:intergenic 40.0%
GGAACTAGATTGGGCAAATT+TGG - chr5.2:70761649-70761668 MS.gene76061:CDS 40.0%
GTATCAGCTTCATACTTCTC+CGG - chr5.2:70762511-70762530 MS.gene76061:CDS 40.0%
GTTCTTGGGATCATTAGCAT+GGG + chr5.2:70761812-70761831 None:intergenic 40.0%
TAATCGCCAATTCTTGCACA+CGG + chr5.2:70761741-70761760 None:intergenic 40.0%
TGTTCTTGGGATCATTAGCA+TGG + chr5.2:70761813-70761832 None:intergenic 40.0%
TTCTAAACCGCTGATCTTGA+TGG + chr5.2:70761778-70761797 None:intergenic 40.0%
TTGGAATTCAATGCTCAGAC+CGG - chr5.2:70762412-70762431 MS.gene76061:CDS 40.0%
TTTGGTAAAACCCAGAAAGG+TGG + chr5.2:70762697-70762716 None:intergenic 40.0%
! AGGACATAGCTTTTCATGGA+AGG - chr5.2:70762299-70762318 MS.gene76061:CDS 40.0%
ATTAGAGGGAGACTTGTGGA+TGG - chr5.2:70762345-70762364 MS.gene76061:CDS 45.0%
CTATTGTTGCAAGCCAACCA+TGG + chr5.2:70761922-70761941 None:intergenic 45.0%
CTCCATACATAAGTCCAGCA+TGG + chr5.2:70762154-70762173 None:intergenic 45.0%
CTTATGTATGGAGCTTGAGC+TGG - chr5.2:70762161-70762180 MS.gene76061:CDS 45.0%
GAGCTGGAGTCATTCCTTTA+TGG - chr5.2:70762177-70762196 MS.gene76061:CDS 45.0%
GTATATGATCGATCGAGGGT+TGG + chr5.2:70762004-70762023 None:intergenic 45.0%
GTTGTGGATTTAGCCATGGT+TGG - chr5.2:70761906-70761925 MS.gene76061:CDS 45.0%
TCGAGGGTTGGAAGACTAAT+AGG + chr5.2:70761992-70762011 None:intergenic 45.0%
! TTTATCTGAGGTCGGGTACA+AGG - chr5.2:70762027-70762046 MS.gene76061:CDS 45.0%
GGTAAAACCCAGAAAGGTGG+TGG + chr5.2:70762694-70762713 None:intergenic 50.0%
TAAAAGCGGGCGAACCATGC+TGG - chr5.2:70762137-70762156 MS.gene76061:CDS 55.0%
TTGGCGCCGTGTGCAAGAAT+TGG - chr5.2:70761732-70761751 MS.gene76061:CDS 55.0%
AGCGGCAGCCACCACCTTTC+TGG - chr5.2:70762683-70762702 MS.gene76061:CDS 65.0%
GCGGCAGCCACCACCTTTCT+GGG - chr5.2:70762684-70762703 MS.gene76061:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 70761647 70762726 70761647 ID=MS.gene76061
chr5.2 mRNA 70761647 70762726 70761647 ID=MS.gene76061.t1;Parent=MS.gene76061
chr5.2 exon 70761647 70762726 70761647 ID=MS.gene76061.t1.exon1;Parent=MS.gene76061.t1
chr5.2 CDS 70761647 70762726 70761647 ID=cds.MS.gene76061.t1;Parent=MS.gene76061.t1
Gene Sequence

>MS.gene76061

ATGGAACTAGATTGGGCAAATTTGGAAGCACTTGCACTTGATTTAATTCTTGATAAGTTGGAAGAACAAATAGATCACATTTGTTTTGGCGCCGTGTGCAAGAATTGGCGATTAATTGCAAAGCTTAACCATCAAGATCAGCGGTTTAGAACTCATGTACTACCCATGCTAATGATCCCAAGAACAGAAAGATGTTTGTATAGCATTTCAGCCAAAAAAATGTATCCATTTAAATTGCCAAATTCTTATTACCATATTTGTTGTGGATTTAGCCATGGTTGGCTTGCAACAATAGATAAAAGTCATGCCATAACACTATTGCATCCTTCTAAGAATGTTGCTCCTATTAGTCTTCCAACCCTCGATCGATCATATACAAGTTTATCTGAGGTCGGGTACAAGGTCACTCTTTCTGCTGACCCTATAACAAGACCAAATGATTATATGGTTGCGGCAATTCACACTAGCATTCATGGATGTTTCGTTTTTATAAAAGCGGGCGAACCATGCTGGACTTATGTATGGAGCTTGAGCTGGAGTCATTCCTTTATGGATGTTATGTTCTATGGAGGTTTAGTTTATGTTGCTAGTAAATATCACGGTATAGTATCTTTTAATCTTGGTCATAAGAGGATAACTCCAAATCCAATTCAGGACATAGCTTTTCATGGAAGGTTTAAAGGTTATCTTGTGAAATCATTAGAGGGAGACTTGTGGATGGTGAAAAGATTTTTTACTTTTAAGATTGATTTCCAAGTGTACAAGTTGGAATTCAATGCTCAGACCGGAAATTTTAAACAAATGCTAAAGCTTGAGAGTCTTGGAGACAATGTTTTGTTCTTAGGAGACACTGATTCAATTTCTGTATCAGCTTCATACTTCTCCGGTTGTCTGAAAAAAGATTCAATTTATTACACAGAAAGTATTAATGCAAACAAGTTAGATTGTGTAACTTGTGGTCCATTTCATATAGGGATATATAATGTAAGAGATGGAACCATTGATCATCATAATCTTCACAATCATTTCTTCAACAAGCGGCAGCCACCACCTTTCTGGGTTTTACCAAATTTCCAATGA

Protein sequence

>MS.gene76061.t1

MELDWANLEALALDLILDKLEEQIDHICFGAVCKNWRLIAKLNHQDQRFRTHVLPMLMIPRTERCLYSISAKKMYPFKLPNSYYHICCGFSHGWLATIDKSHAITLLHPSKNVAPISLPTLDRSYTSLSEVGYKVTLSADPITRPNDYMVAAIHTSIHGCFVFIKAGEPCWTYVWSLSWSHSFMDVMFYGGLVYVASKYHGIVSFNLGHKRITPNPIQDIAFHGRFKGYLVKSLEGDLWMVKRFFTFKIDFQVYKLEFNAQTGNFKQMLKLESLGDNVLFLGDTDSISVSASYFSGCLKKDSIYYTESINANKLDCVTCGPFHIGIYNVRDGTIDHHNLHNHFFNKRQPPPFWVLPNFQ