AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52259

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52259.t1 MTR_7g077620 68.280 372 112 4 1 368 1 370 0.0 512
MS.gene52259.t1 MTR_7g077650 68.966 377 105 6 1 370 1 372 2.08e-179 503
MS.gene52259.t1 MTR_7g077660 66.845 374 114 7 1 370 1 368 6.26e-168 473
MS.gene52259.t1 MTR_7g076110 56.757 296 113 6 86 370 22 313 9.90e-103 306
MS.gene52259.t1 MTR_7g076100 64.286 238 79 4 136 370 66 300 1.84e-96 289
MS.gene52259.t1 MTR_7g076100 77.778 54 11 1 1 53 1 54 4.20e-20 89.7
MS.gene52259.t1 MTR_1g108740 43.045 381 167 15 1 368 1 344 5.32e-84 259
MS.gene52259.t1 MTR_5g038260 42.643 401 176 17 3 370 4 383 4.58e-82 255
MS.gene52259.t1 MTR_5g038230 42.065 397 150 18 5 368 4 353 1.07e-77 243
MS.gene52259.t1 MTR_1g064570 40.573 419 178 18 3 370 2 400 1.06e-71 229
MS.gene52259.t1 MTR_5g082770 45.016 311 132 8 5 302 4 288 6.19e-71 224
MS.gene52259.t1 MTR_1g016150 46.429 196 98 5 1 191 1 194 2.84e-47 160
MS.gene52259.t1 MTR_7g076130 59.375 128 48 2 163 287 22 148 2.89e-43 147
MS.gene52259.t1 MTR_5g082780 39.149 235 111 9 114 334 1 217 1.03e-36 133
MS.gene52259.t1 MTR_1g108750 47.097 155 46 4 5 157 4 124 3.86e-25 99.0
MS.gene52259.t1 MTR_3g114970 24.297 391 228 14 4 366 3 353 8.07e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGTAATCAGAATGATTT+AGG 0.091939 7.4:-28514167 MS.gene52259:CDS
AAAGACCAAACCAATCATTT+TGG 0.176374 7.4:-28514643 MS.gene52259:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7.4:+28514488 None:intergenic
GATGAACAAACTTGAGAGTT+TGG 0.285385 7.4:-28514288 MS.gene52259:CDS
ACACTCTTTCATTGTATTGA+TGG 0.308234 7.4:+28514862 None:intergenic
CAATACAATGAAAGAGTGTT+TGG 0.315097 7.4:-28514858 MS.gene52259:CDS
GAAGGATGGGATAATTGAAT+AGG 0.335830 7.4:+28514884 None:intergenic
TAATCACTTGGGCTTGTAAT+GGG 0.338599 7.4:+28514707 None:intergenic
AAGAACACATAGATCATGTT+AGG 0.348741 7.4:-28515084 MS.gene52259:CDS
CATCCGCCTCTTGTTTCTCC+AGG 0.350422 7.4:-28514220 MS.gene52259:CDS
CGCCTCTTGTTTCTCCAGGT+TGG 0.365401 7.4:-28514216 MS.gene52259:CDS
CCAAGTGATTATGTGGTTGC+AGG 0.365765 7.4:-28514695 MS.gene52259:CDS
CAAATGGGAGCTATACAAAA+AGG 0.367344 7.4:+28514776 None:intergenic
CAGCTATAATAAGATTGTTC+TGG 0.368066 7.4:+28514440 None:intergenic
GATTGTTCTGGATCAATATA+TGG 0.371210 7.4:+28514452 None:intergenic
AGATGCATATCCTCTTAGTT+TGG 0.376506 7.4:-28515122 MS.gene52259:CDS
TAGCAATGAGGAAGGTTGAT+TGG 0.381788 7.4:-28515153 None:intergenic
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7.4:+28514489 None:intergenic
CTCCAGGTTGGAAAAGGATT+CGG 0.393107 7.4:-28514204 MS.gene52259:CDS
GGTTGCAGGGATTTACCAAT+TGG 0.397037 7.4:-28514681 MS.gene52259:CDS
AAGGTAATCAGAATGATTTA+GGG 0.402253 7.4:-28514166 MS.gene52259:CDS
CCATGGTTGGCTTGCACTAG+TGG 0.416361 7.4:-28514828 MS.gene52259:CDS
GGGTTGTACCACAATTTCAA+TGG 0.418918 7.4:-28514064 MS.gene52259:CDS
CGAATCCAAAATGATTGGTT+TGG 0.419723 7.4:+28514638 None:intergenic
CACATAGATCATGTTAGGTT+TGG 0.419733 7.4:-28515079 MS.gene52259:CDS
ACATGTTCACAAATTGCAAC+TGG 0.426873 7.4:-28514336 MS.gene52259:CDS
TTGTTTCTCCAGGTTGGAAA+AGG 0.426990 7.4:-28514210 MS.gene52259:CDS
TTACACGAATCCAAAATGAT+TGG 0.441586 7.4:+28514633 None:intergenic
TTGGAGAGGTGGCAAACAAA+TGG 0.451491 7.4:+28514760 None:intergenic
TTGTGTGTATACGATAATAT+TGG 0.456492 7.4:+28514960 None:intergenic
ACCTCTCCAAGTTGTCGATA+AGG 0.466729 7.4:-28514747 MS.gene52259:CDS
TGGATTCGTGTAAACAGTAA+TGG 0.467032 7.4:-28514623 MS.gene52259:CDS
AGCAATGAGGAAGGTTGATT+GGG 0.467550 7.4:-28515152 None:intergenic
AATACAATGAAAGAGTGTTT+GGG 0.472961 7.4:-28514857 MS.gene52259:CDS
CCTGCAACCACATAATCACT+TGG 0.476124 7.4:+28514695 None:intergenic
ATAATCACTTGGGCTTGTAA+TGG 0.476686 7.4:+28514706 None:intergenic
GGTTGTACCACAATTTCAAT+GGG 0.477703 7.4:-28514063 MS.gene52259:CDS
TGAAAGCTAGACGACCCAAT+TGG 0.478175 7.4:+28514666 None:intergenic
TAGTTTGGTTCTTGATAAGT+TGG 0.478359 7.4:-28515107 MS.gene52259:CDS
AAGGATGGGATAATTGAATA+GGG 0.479464 7.4:+28514885 None:intergenic
AATCATTGACCGGAGACATA+TGG 0.484616 7.4:-28514400 MS.gene52259:CDS
CATTGTATTGATGGAAGGAT+GGG 0.492580 7.4:+28514871 None:intergenic
CCTTACCACTTTCCTTCGAG+TGG 0.492809 7.4:-28514100 MS.gene52259:CDS
TCATTGTATTGATGGAAGGA+TGG 0.495017 7.4:+28514870 None:intergenic
GTTGCAGGGATTTACCAATT+GGG 0.498427 7.4:-28514680 MS.gene52259:CDS
TACAAGCCCAAGTGATTATG+TGG 0.499442 7.4:-28514702 MS.gene52259:CDS
GTTTGGGCTGTAGCCATGGT+TGG 0.500641 7.4:-28514841 MS.gene52259:CDS
AGGAGGATTGTTGATCTTGA+AGG 0.506138 7.4:+28514529 None:intergenic
AAAAGGATTCACTAGAGTTA+CGG 0.507550 7.4:+28514793 None:intergenic
GAGATCTATAATGTGAAGGA+TGG 0.509549 7.4:-28514140 MS.gene52259:CDS
TTATCAAGAACCAAACTAAG+AGG 0.515747 7.4:+28515112 None:intergenic
TCTTTCATTGTATTGATGGA+AGG 0.518852 7.4:+28514866 None:intergenic
TGGAGAGGTGGCAAACAAAT+GGG 0.522942 7.4:+28514761 None:intergenic
TCTCACCATCCATATGTCTC+CGG 0.543961 7.4:+28514391 None:intergenic
TAATCAGAATGATTTAGGGT+TGG 0.544184 7.4:-28514162 MS.gene52259:CDS
TATTTCGTGAAATCATTGAC+CGG 0.545774 7.4:-28514410 MS.gene52259:CDS
CAAGTGATTATGTGGTTGCA+GGG 0.562886 7.4:-28514694 MS.gene52259:CDS
TGTAATGGGGTCAGCAGACA+AGG 0.568289 7.4:+28514721 None:intergenic
TGCATCCACTCGAAGGAAAG+TGG 0.571214 7.4:+28514095 None:intergenic
GAGTGTTTGGGCTGTAGCCA+TGG 0.571885 7.4:-28514845 MS.gene52259:CDS
CCACTCGAAGGAAAGTGGTA+AGG 0.582043 7.4:+28514100 None:intergenic
GGTCAATCCCATTGAAATTG+TGG 0.583167 7.4:+28514056 None:intergenic
AGGTAACCTTATCGACAACT+TGG 0.584079 7.4:+28514741 None:intergenic
GTTGATCTTGAAGGATACAA+TGG 0.595504 7.4:+28514538 None:intergenic
GTTGGAGATCTATAATGTGA+AGG 0.603732 7.4:-28514144 MS.gene52259:CDS
AATCACTTGGGCTTGTAATG+GGG 0.606028 7.4:+28514708 None:intergenic
TACGATAATATTGGTCTGAG+AGG 0.624334 7.4:+28514969 None:intergenic
CCACTAGTGCAAGCCAACCA+TGG 0.626331 7.4:+28514828 None:intergenic
CTGCAACCACATAATCACTT+GGG 0.636696 7.4:+28514696 None:intergenic
TTCCAACCTGGAGAAACAAG+AGG 0.642260 7.4:+28514214 None:intergenic
TTATCGACAACTTGGAGAGG+TGG 0.643191 7.4:+28514749 None:intergenic
TCTGAGAGGATCATCAACAT+AGG 0.649847 7.4:+28514983 None:intergenic
TATACACACAATAACACAGA+CGG 0.650451 7.4:-28514948 MS.gene52259:CDS
GAGAGGATCATCAACATAGG+TGG 0.651968 7.4:+28514986 None:intergenic
ACCTTATCGACAACTTGGAG+AGG 0.658746 7.4:+28514746 None:intergenic
ATGATTTCACGAAATAAGCA+CGG 0.661141 7.4:+28514417 None:intergenic
ATGCAGAAATTGAATCACCA+TGG 0.661152 7.4:+28514240 None:intergenic
TTGCAACTGGATACCCAGAG+TGG 0.681453 7.4:-28514323 MS.gene52259:CDS
ATTGACCGGAGACATATGGA+TGG 0.696827 7.4:-28514396 MS.gene52259:CDS
GGGTCATTAGAGTCATCACA+AGG 0.703659 7.4:+28514509 None:intergenic
CAACCTGGAGAAACAAGAGG+CGG 0.708209 7.4:+28514217 None:intergenic
TCATTAGAGTCATCACAAGG+AGG 0.748745 7.4:+28514512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCATAAAAATCATCAATTT+AGG - chr7.4:28514188-28514207 MS.gene52259:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG + chr7.4:28514720-28514739 None:intergenic 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG + chr7.4:28514721-28514740 None:intergenic 20.0%
!!! ACTGATGTTGTATTTTATAA+AGG - chr7.4:28514616-28514635 MS.gene52259:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr7.4:28514192-28514211 None:intergenic 20.0%
! AAGGTAATCAGAATGATTTA+GGG - chr7.4:28515040-28515059 MS.gene52259:CDS 25.0%
! TTGTGTGTATACGATAATAT+TGG + chr7.4:28514249-28514268 None:intergenic 25.0%
!!! AATACAATGAAAGAGTGTTT+GGG - chr7.4:28514349-28514368 MS.gene52259:CDS 25.0%
!!! TCGGTTTATTTTGTTTATCA+AGG - chr7.4:28515021-28515040 MS.gene52259:CDS 25.0%
AAAAGGATTCACTAGAGTTA+CGG + chr7.4:28514416-28514435 None:intergenic 30.0%
AAGAACACATAGATCATGTT+AGG - chr7.4:28514122-28514141 MS.gene52259:CDS 30.0%
AAGGATGGGATAATTGAATA+GGG + chr7.4:28514324-28514343 None:intergenic 30.0%
ACACTCTTTCATTGTATTGA+TGG + chr7.4:28514347-28514366 None:intergenic 30.0%
ATGATTTCACGAAATAAGCA+CGG + chr7.4:28514792-28514811 None:intergenic 30.0%
CAAGGTAATCAGAATGATTT+AGG - chr7.4:28515039-28515058 MS.gene52259:CDS 30.0%
CAGCTATAATAAGATTGTTC+TGG + chr7.4:28514769-28514788 None:intergenic 30.0%
TATACACACAATAACACAGA+CGG - chr7.4:28514258-28514277 MS.gene52259:CDS 30.0%
TATTTCGTGAAATCATTGAC+CGG - chr7.4:28514796-28514815 MS.gene52259:CDS 30.0%
TTACACGAATCCAAAATGAT+TGG + chr7.4:28514576-28514595 None:intergenic 30.0%
TTATCAAGAACCAAACTAAG+AGG + chr7.4:28514097-28514116 None:intergenic 30.0%
TTGGTGTAGTGTGTAAAAAT+TGG - chr7.4:28514146-28514165 MS.gene52259:CDS 30.0%
! AAAGACCAAACCAATCATTT+TGG - chr7.4:28514563-28514582 MS.gene52259:CDS 30.0%
! GATTGTTCTGGATCAATATA+TGG + chr7.4:28514757-28514776 None:intergenic 30.0%
! TAATCAGAATGATTTAGGGT+TGG - chr7.4:28515044-28515063 MS.gene52259:CDS 30.0%
! TCTTTCATTGTATTGATGGA+AGG + chr7.4:28514343-28514362 None:intergenic 30.0%
!! ATTTTATTTGTCGCAAACCA+TGG - chr7.4:28514949-28514968 MS.gene52259:CDS 30.0%
!! CAATACAATGAAAGAGTGTT+TGG - chr7.4:28514348-28514367 MS.gene52259:CDS 30.0%
!! TAGTTTGGTTCTTGATAAGT+TGG - chr7.4:28514099-28514118 MS.gene52259:CDS 30.0%
ACATGTTCACAAATTGCAAC+TGG - chr7.4:28514870-28514889 MS.gene52259:CDS 35.0%
AGATGCATATCCTCTTAGTT+TGG - chr7.4:28514084-28514103 MS.gene52259:CDS 35.0%
ATAATCACTTGGGCTTGTAA+TGG + chr7.4:28514503-28514522 None:intergenic 35.0%
ATGCAGAAATTGAATCACCA+TGG + chr7.4:28514969-28514988 None:intergenic 35.0%
CAAATGGGAGCTATACAAAA+AGG + chr7.4:28514433-28514452 None:intergenic 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.4:28514127-28514146 MS.gene52259:CDS 35.0%
GAAGGATGGGATAATTGAAT+AGG + chr7.4:28514325-28514344 None:intergenic 35.0%
GAGATCTATAATGTGAAGGA+TGG - chr7.4:28515066-28515085 MS.gene52259:CDS 35.0%
GATGAACAAACTTGAGAGTT+TGG - chr7.4:28514918-28514937 MS.gene52259:CDS 35.0%
GGTTGTACCACAATTTCAAT+GGG - chr7.4:28515143-28515162 MS.gene52259:CDS 35.0%
GTTGATCTTGAAGGATACAA+TGG + chr7.4:28514671-28514690 None:intergenic 35.0%
GTTGGAGATCTATAATGTGA+AGG - chr7.4:28515062-28515081 MS.gene52259:CDS 35.0%
TAATCACTTGGGCTTGTAAT+GGG + chr7.4:28514502-28514521 None:intergenic 35.0%
TGGATTCGTGTAAACAGTAA+TGG - chr7.4:28514583-28514602 MS.gene52259:CDS 35.0%
! CATTGTATTGATGGAAGGAT+GGG + chr7.4:28514338-28514357 None:intergenic 35.0%
! CGAATCCAAAATGATTGGTT+TGG + chr7.4:28514571-28514590 None:intergenic 35.0%
! TACGATAATATTGGTCTGAG+AGG + chr7.4:28514240-28514259 None:intergenic 35.0%
! TCATTGTATTGATGGAAGGA+TGG + chr7.4:28514339-28514358 None:intergenic 35.0%
AAAAAGTTGCATCCACTCGA+AGG + chr7.4:28515121-28515140 None:intergenic 40.0%
AATCACTTGGGCTTGTAATG+GGG + chr7.4:28514501-28514520 None:intergenic 40.0%
AATCATTGACCGGAGACATA+TGG - chr7.4:28514806-28514825 MS.gene52259:CDS 40.0%
AGGAGGATTGTTGATCTTGA+AGG + chr7.4:28514680-28514699 None:intergenic 40.0%
AGGTAACCTTATCGACAACT+TGG + chr7.4:28514468-28514487 None:intergenic 40.0%
CAAGTGATTATGTGGTTGCA+GGG - chr7.4:28514512-28514531 MS.gene52259:CDS 40.0%
CTGCAACCACATAATCACTT+GGG + chr7.4:28514513-28514532 None:intergenic 40.0%
GGGTTGTACCACAATTTCAA+TGG - chr7.4:28515142-28515161 MS.gene52259:CDS 40.0%
GTTGCAGGGATTTACCAATT+GGG - chr7.4:28514526-28514545 MS.gene52259:CDS 40.0%
TACAAGCCCAAGTGATTATG+TGG - chr7.4:28514504-28514523 MS.gene52259:CDS 40.0%
TCATTAGAGTCATCACAAGG+AGG + chr7.4:28514697-28514716 None:intergenic 40.0%
TCTGAGAGGATCATCAACAT+AGG + chr7.4:28514226-28514245 None:intergenic 40.0%
! TTGTTTCTCCAGGTTGGAAA+AGG - chr7.4:28514996-28515015 MS.gene52259:CDS 40.0%
!! CTGTTCAAGTTTTCCACTCT+GGG + chr7.4:28514899-28514918 None:intergenic 40.0%
!! TCTGTTCAAGTTTTCCACTC+TGG + chr7.4:28514900-28514919 None:intergenic 40.0%
!!! CGAGTGGATGCAACTTTTTT+GGG - chr7.4:28515122-28515141 MS.gene52259:CDS 40.0%
!!! TCGAGTGGATGCAACTTTTT+TGG - chr7.4:28515121-28515140 MS.gene52259:CDS 40.0%
ACCTCTCCAAGTTGTCGATA+AGG - chr7.4:28514459-28514478 MS.gene52259:CDS 45.0%
ACCTTATCGACAACTTGGAG+AGG + chr7.4:28514463-28514482 None:intergenic 45.0%
ATTGACCGGAGACATATGGA+TGG - chr7.4:28514810-28514829 MS.gene52259:CDS 45.0%
CCAAGTGATTATGTGGTTGC+AGG - chr7.4:28514511-28514530 MS.gene52259:CDS 45.0%
CCTGCAACCACATAATCACT+TGG + chr7.4:28514514-28514533 None:intergenic 45.0%
GAGAGGATCATCAACATAGG+TGG + chr7.4:28514223-28514242 None:intergenic 45.0%
GGGTCATTAGAGTCATCACA+AGG + chr7.4:28514700-28514719 None:intergenic 45.0%
GGTTGCAGGGATTTACCAAT+TGG - chr7.4:28514525-28514544 MS.gene52259:CDS 45.0%
TCTCACCATCCATATGTCTC+CGG + chr7.4:28514818-28514837 None:intergenic 45.0%
TGAAAGCTAGACGACCCAAT+TGG + chr7.4:28514543-28514562 None:intergenic 45.0%
TGGAGAGGTGGCAAACAAAT+GGG + chr7.4:28514448-28514467 None:intergenic 45.0%
TTATCGACAACTTGGAGAGG+TGG + chr7.4:28514460-28514479 None:intergenic 45.0%
TTCCAACCTGGAGAAACAAG+AGG + chr7.4:28514995-28515014 None:intergenic 45.0%
TTGGAGAGGTGGCAAACAAA+TGG + chr7.4:28514449-28514468 None:intergenic 45.0%
! AACCGAATCCTTTTCCAACC+TGG + chr7.4:28515007-28515026 None:intergenic 45.0%
! CTCCAGGTTGGAAAAGGATT+CGG - chr7.4:28515002-28515021 MS.gene52259:CDS 45.0%
CAACCTGGAGAAACAAGAGG+CGG + chr7.4:28514992-28515011 None:intergenic 50.0%
CCACTCGAAGGAAAGTGGTA+AGG + chr7.4:28515109-28515128 None:intergenic 50.0%
CCTTACCACTTTCCTTCGAG+TGG - chr7.4:28515106-28515125 MS.gene52259:CDS 50.0%
TGCATCCACTCGAAGGAAAG+TGG + chr7.4:28515114-28515133 None:intergenic 50.0%
TTGCAACTGGATACCCAGAG+TGG - chr7.4:28514883-28514902 MS.gene52259:CDS 50.0%
! TGTAATGGGGTCAGCAGACA+AGG + chr7.4:28514488-28514507 None:intergenic 50.0%
CCACTAGTGCAAGCCAACCA+TGG + chr7.4:28514381-28514400 None:intergenic 55.0%
GAGTGTTTGGGCTGTAGCCA+TGG - chr7.4:28514361-28514380 MS.gene52259:CDS 55.0%
GTTTGGGCTGTAGCCATGGT+TGG - chr7.4:28514365-28514384 MS.gene52259:CDS 55.0%
! CATCCGCCTCTTGTTTCTCC+AGG - chr7.4:28514986-28515005 MS.gene52259:CDS 55.0%
! CGCCTCTTGTTTCTCCAGGT+TGG - chr7.4:28514990-28515009 MS.gene52259:CDS 55.0%
!! CCATGGTTGGCTTGCACTAG+TGG - chr7.4:28514378-28514397 MS.gene52259:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 28514058 28515170 28514058 ID=MS.gene52259
chr7.4 mRNA 28514058 28515170 28514058 ID=MS.gene52259.t1;Parent=MS.gene52259
chr7.4 exon 28514058 28515170 28514058 ID=MS.gene52259.t1.exon1;Parent=MS.gene52259.t1
chr7.4 CDS 28514058 28515170 28514058 ID=cds.MS.gene52259.t1;Parent=MS.gene52259.t1
Gene Sequence

>MS.gene52259

ATGAGGAAGGTTGATTGGGAAAATTTAGATGCATATCCTCTTAGTTTGGTTCTTGATAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGTGTAGTGTGTAAAAATTGGCTTTCAGTTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAATATACCACCTATGTTGATGATCCTCTCAGACCAATATTATCGTATACACACAATAACACAGACGGAGAAGAGATCGTTGTATAGTATTATAAGTGAGAAAGAGTACCCTATTCAATTATCCCATCCTTCCATCAATACAATGAAAGAGTGTTTGGGCTGTAGCCATGGTTGGCTTGCACTAGTGGATTATAACAATGCCGTAACTCTAGTGAATCCTTTTTGTATAGCTCCCATTTGTTTGCCACCTCTCCAAGTTGTCGATAAGGTTACCTTGTCTGCTGACCCCATTACAAGCCCAAGTGATTATGTGGTTGCAGGGATTTACCAATTGGGTCGTCTAGCTTTCAAAAGACCAAACCAATCATTTTGGATTCGTGTAAACAGTAATGGATTTCATTTCACTGATGTTGTATTTTATAAAGGTCTAATATTTGCTGACAGCGTTGAAGCTACCATTGTATCCTTCAAGATCAACAATCCTCCTTGTGATGACTCTAATGACCCTAATTTTACTTACTATGAGAAGATATCTAGCACTCCATATATTGATCCAGAACAATCTTATTATAGCTGCCGTGCTTATTTCGTGAAATCATTGACCGGAGACATATGGATGGTGAGAAGATATAAAATCGACGAGCATTCATTCAAACTACATGTTCACAAATTGCAACTGGATACCCAGAGTGGAAAACTTGAACAGATGAACAAACTTGAGAGTTTGGAAAACAACATTTTATTTGTCGCAAACCATGGTGATTCAATTTCTGCATCCGCCTCTTGTTTCTCCAGGTTGGAAAAGGATTCGGTTTATTTTGTTTATCAAGGTAATCAGAATGATTTAGGGTTGGAGATCTATAATGTGAAGGATGGAAGCTTTCAGAGACTATCCTTACCACTTTCCTTCGAGTGGATGCAACTTTTTTGGGTTGTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene52259.t1

MRKVDWENLDAYPLSLVLDKLEEHIDHVRFGVVCKNWLSVAKLNHKNHQFRINIPPMLMILSDQYYRIHTITQTEKRSLYSIISEKEYPIQLSHPSINTMKECLGCSHGWLALVDYNNAVTLVNPFCIAPICLPPLQVVDKVTLSADPITSPSDYVVAGIYQLGRLAFKRPNQSFWIRVNSNGFHFTDVVFYKGLIFADSVEATIVSFKINNPPCDDSNDPNFTYYEKISSTPYIDPEQSYYSCRAYFVKSLTGDIWMVRRYKIDEHSFKLHVHKLQLDTQSGKLEQMNKLESLENNILFVANHGDSISASASCFSRLEKDSVYFVYQGNQNDLGLEIYNVKDGSFQRLSLPLSFEWMQLFWVVPQFQWD