AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27636

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27636.t1 MTR_7g076110 94.888 313 16 0 58 370 1 313 0.0 586
MS.gene27636.t1 MTR_7g077620 56.464 379 145 8 1 368 1 370 7.82e-134 388
MS.gene27636.t1 MTR_7g077650 53.886 386 148 12 1 370 1 372 2.70e-122 358
MS.gene27636.t1 MTR_7g077660 53.457 376 154 9 5 370 4 368 6.49e-119 349
MS.gene27636.t1 MTR_7g076100 60.504 238 90 4 134 370 66 300 1.76e-91 276
MS.gene27636.t1 MTR_7g076100 83.333 54 9 0 1 54 1 54 1.91e-24 102
MS.gene27636.t1 MTR_5g038260 39.175 388 203 14 6 370 6 383 6.58e-74 234
MS.gene27636.t1 MTR_1g108740 42.627 373 177 14 3 368 2 344 2.26e-73 232
MS.gene27636.t1 MTR_5g082770 44.737 304 142 9 6 302 4 288 6.59e-73 229
MS.gene27636.t1 MTR_5g038230 36.693 387 184 13 6 368 4 353 1.26e-66 214
MS.gene27636.t1 MTR_1g064570 38.592 412 193 16 5 370 3 400 1.04e-65 214
MS.gene27636.t1 MTR_1g016150 45.550 191 74 7 3 175 2 180 9.47e-42 146
MS.gene27636.t1 MTR_7g076130 56.349 126 53 2 163 287 24 148 6.86e-39 136
MS.gene27636.t1 MTR_5g082780 38.667 225 108 8 109 325 6 208 6.70e-33 123
MS.gene27636.t1 MTR_1g108750 44.000 150 53 4 6 154 4 123 1.91e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCAAACCAACCATTT+TGG 0.209572 7.2:-29686762 MS.gene27636:CDS
GACAGTGTTCCAGAAGATTT+AGG 0.219883 7.2:-29686280 MS.gene27636:CDS
TTCTCATAGTAGGTAAAATT+AGG 0.225887 7.2:+29686604 None:intergenic
ACCATCAAAACCATCAATTT+AGG 0.249142 7.2:-29687128 MS.gene27636:CDS
ACCTGTCATGGCTCTTCATT+TGG 0.288626 7.2:-29686988 MS.gene27636:CDS
ATCCCAAACCCTAAATCTTC+TGG 0.300792 7.2:+29686271 None:intergenic
GGAATAATACCATACTTAAA+AGG 0.315387 7.2:+29686919 None:intergenic
CGACGTTTACAAATTGAAAC+TGG 0.332871 7.2:-29686449 MS.gene27636:CDS
GTTCCAGAAGATTTAGGGTT+TGG 0.341176 7.2:-29686274 MS.gene27636:CDS
AATGAACAGACTTAAGAGTT+TGG 0.348149 7.2:-29686401 MS.gene27636:CDS
AGAAGAACTTCCTCTTAGTT+TGG 0.350191 7.2:-29687232 MS.gene27636:CDS
TTCCAGAAGATTTAGGGTTT+GGG 0.356926 7.2:-29686273 MS.gene27636:CDS
AGTACTCTTTGTGGCTTAGA+AGG 0.357548 7.2:+29687040 None:intergenic
GTCATGGCTCTTCATTTGGT+TGG 0.362670 7.2:-29686984 MS.gene27636:CDS
GTAACTATAGGAATCCAAAA+TGG 0.366794 7.2:+29686748 None:intergenic
TGTAATAAATTTGTAACTAT+AGG 0.375568 7.2:+29686736 None:intergenic
ACAGTGTTCCAGAAGATTTA+GGG 0.377757 7.2:-29686279 MS.gene27636:CDS
ACACTCCGGAGATATCTTAA+AGG 0.378694 7.2:+29686865 None:intergenic
AAGTTTGTCCAGCATAATCT+TGG 0.388756 7.2:+29686551 None:intergenic
AAAAGGATTGAGTAGAGTTA+TGG 0.390478 7.2:+29686936 None:intergenic
GGGCTCTACCTCAATTTCAA+TGG 0.397463 7.2:-29686177 MS.gene27636:CDS
CACATAGATCATCTTAGATT+CGG 0.408172 7.2:-29687189 MS.gene27636:CDS
ATGAACAGACTTAAGAGTTT+GGG 0.410853 7.2:-29686400 MS.gene27636:CDS
GGCTCTACCTCAATTTCAAT+GGG 0.416041 7.2:-29686176 MS.gene27636:CDS
TCTCATAGTAGGTAAAATTA+GGG 0.423599 7.2:+29686605 None:intergenic
GCTAGTTATCTTCTCATAGT+AGG 0.423741 7.2:+29686594 None:intergenic
TACTGCTCTTGGATTCGATT+GGG 0.430675 7.2:+29687076 None:intergenic
ATTGAATGGAGTACTCTTTG+TGG 0.430687 7.2:+29687031 None:intergenic
ACGACAGCAACATAATCATC+TGG 0.430984 7.2:+29686814 None:intergenic
TTCGAGAGGTGGCAGACTAA+TGG 0.431252 7.2:+29686894 None:intergenic
TTAGGGTCATTTGAGCGATC+AGG 0.435005 7.2:+29686622 None:intergenic
TCGAGAGGTGGCAGACTAAT+GGG 0.436341 7.2:+29686895 None:intergenic
GACATCGAAAAGCTTTCATA+TGG 0.441021 7.2:-29686475 MS.gene27636:CDS
GTATAGTATTCCTAAATTGA+TGG 0.447518 7.2:+29687118 None:intergenic
CGATGAGGAAGGTGGTAGAC+TGG 0.455468 7.2:-29687263 MS.gene27636:CDS
TATCTTGTGAAATCATTGAA+TGG 0.459383 7.2:-29686529 MS.gene27636:CDS
GATTGGGACGCTCATGATCA+TGG 0.463327 7.2:+29687092 None:intergenic
AATCAATCCCATTGAAATTG+AGG 0.474667 7.2:+29686169 None:intergenic
GCTGTCGTCATTTACAACTT+TGG 0.475418 7.2:-29686799 MS.gene27636:CDS
TAATCATCTGGGCTTATGAT+GGG 0.479866 7.2:+29686826 None:intergenic
ATACAAGGATCTACTGCTCT+TGG 0.483271 7.2:+29687065 None:intergenic
ATAATCATCTGGGCTTATGA+TGG 0.483695 7.2:+29686825 None:intergenic
CTACTGCTCTTGGATTCGAT+TGG 0.489995 7.2:+29687075 None:intergenic
TAGGGTCATTTGAGCGATCA+GGG 0.500420 7.2:+29686623 None:intergenic
AATCATTGAATGGAGACATA+TGG 0.507395 7.2:-29686519 MS.gene27636:CDS
AGTTTGTCCAGCATAATCTT+GGG 0.508944 7.2:+29686552 None:intergenic
TTGTCAAGAACCAAACTAAG+AGG 0.509205 7.2:+29687222 None:intergenic
TCGGTTTCGTGTGCAAGACT+TGG 0.511651 7.2:-29687170 MS.gene27636:CDS
AGGAGGAGTGTTGAACTTGA+AGG 0.513586 7.2:+29686648 None:intergenic
ACAAGAGGCAGACACAGAAA+AGG 0.516829 7.2:+29686342 None:intergenic
TGTGATGGATGAAAATTGAA+TGG 0.520449 7.2:+29687017 None:intergenic
CTATAGGAATCCAAAATGGT+TGG 0.542954 7.2:+29686752 None:intergenic
CACCACCTTTAAGATATCTC+CGG 0.544228 7.2:-29686870 MS.gene27636:CDS
GGGATCTATAATGTGAAGGA+TGG 0.545871 7.2:-29686253 MS.gene27636:CDS
ATTGGGACGCTCATGATCAT+GGG 0.549814 7.2:+29687093 None:intergenic
ACCAAATGAAGAGCCATGAC+AGG 0.552193 7.2:+29686987 None:intergenic
GCTAGAGCCCAAGATTATGC+TGG 0.553236 7.2:-29686559 MS.gene27636:CDS
TGCGTCTGCTCGAAGGAAAG+TGG 0.572553 7.2:+29686208 None:intergenic
GTGGCTTAGAAGGCTATACA+AGG 0.574812 7.2:+29687050 None:intergenic
ATCACATTTATAACCTGTCA+TGG 0.576549 7.2:-29687000 MS.gene27636:CDS
ATCTGGGCTTATGATGGGGT+CGG 0.585200 7.2:+29686831 None:intergenic
GATGAGGAAGGTGGTAGACT+GGG 0.589322 7.2:-29687262 MS.gene27636:CDS
CTTTCATTAGTCCACAGTAA+CGG 0.591490 7.2:-29686961 MS.gene27636:CDS
CGACAGCAACATAATCATCT+GGG 0.592571 7.2:+29686815 None:intergenic
TTTGAACTTGGAGAAACAAG+AGG 0.593858 7.2:+29686327 None:intergenic
ATGACAGGTTATAAATGTGA+TGG 0.594341 7.2:+29687002 None:intergenic
GGGTCATTTGAGCGATCAGG+GGG 0.608939 7.2:+29686625 None:intergenic
AAGTTTCTTAACGATGAGGA+AGG 0.609754 7.2:-29687274 None:intergenic
AAAGGTGGTGATTCGAGAGG+TGG 0.610801 7.2:+29686883 None:intergenic
GTTTGGGATCTATAATGTGA+AGG 0.626362 7.2:-29686257 MS.gene27636:CDS
TTTCTTAACGATGAGGAAGG+TGG 0.631528 7.2:-29687271 None:intergenic
AATCATCTGGGCTTATGATG+GGG 0.632274 7.2:+29686827 None:intergenic
AGGGTCATTTGAGCGATCAG+GGG 0.648257 7.2:+29686624 None:intergenic
CTCCGGAGATATCTTAAAGG+TGG 0.652726 7.2:+29686868 None:intergenic
CGGCAGACAATGTAACACTC+CGG 0.665324 7.2:+29686851 None:intergenic
GAGTTATGGAACCGTTACTG+TGG 0.666466 7.2:+29686950 None:intergenic
GGGACGCTCATGATCATGGG+TGG 0.671811 7.2:+29687096 None:intergenic
CTTAAAGGTGGTGATTCGAG+AGG 0.674686 7.2:+29686880 None:intergenic
ATTGAATGGAGACATATGGA+TGG 0.693585 7.2:-29686515 MS.gene27636:CDS
TTGAAACTGGACACCCAGAG+TGG 0.696035 7.2:-29686436 MS.gene27636:CDS
GGATTGAATAGTGTCTGAGT+GGG 0.704793 7.2:+29686669 None:intergenic
AGGATTGAATAGTGTCTGAG+TGG 0.717909 7.2:+29686668 None:intergenic
AGAGGTGGCAGACTAATGGG+AGG 0.718242 7.2:+29686898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATAAATTTGTAACTAT+AGG + chr7.2:29686699-29686718 None:intergenic 15.0%
!!! AATGATGTTGTATTTTATAA+AGG - chr7.2:29686723-29686742 MS.gene27636:CDS 15.0%
!!! ATTTTATTTATCGGATATTG+TGG - chr7.2:29687062-29687081 MS.gene27636:CDS 20.0%
! GGAATAATACCATACTTAAA+AGG + chr7.2:29686516-29686535 None:intergenic 25.0%
! GTATAGTATTCCTAAATTGA+TGG + chr7.2:29686317-29686336 None:intergenic 25.0%
! TATCTTGTGAAATCATTGAA+TGG - chr7.2:29686903-29686922 MS.gene27636:CDS 25.0%
! TCTCATAGTAGGTAAAATTA+GGG + chr7.2:29686830-29686849 None:intergenic 25.0%
! TTCTCATAGTAGGTAAAATT+AGG + chr7.2:29686831-29686850 None:intergenic 25.0%
!!! AACTGAATCTTTTTTGAACT+TGG + chr7.2:29687120-29687139 None:intergenic 25.0%
!!! GGAGACAACATTTTATTTAT+CGG - chr7.2:29687053-29687072 MS.gene27636:CDS 25.0%
AAAAGGATTGAGTAGAGTTA+TGG + chr7.2:29686499-29686518 None:intergenic 30.0%
AATCATTGAATGGAGACATA+TGG - chr7.2:29686913-29686932 MS.gene27636:CDS 30.0%
AATGAACAGACTTAAGAGTT+TGG - chr7.2:29687031-29687050 MS.gene27636:CDS 30.0%
ACCATCAAAACCATCAATTT+AGG - chr7.2:29686304-29686323 MS.gene27636:CDS 30.0%
ATCACATTTATAACCTGTCA+TGG - chr7.2:29686432-29686451 MS.gene27636:CDS 30.0%
ATGAACAGACTTAAGAGTTT+GGG - chr7.2:29687032-29687051 MS.gene27636:CDS 30.0%
ATGACAGGTTATAAATGTGA+TGG + chr7.2:29686433-29686452 None:intergenic 30.0%
CACATAGATCATCTTAGATT+CGG - chr7.2:29686243-29686262 MS.gene27636:CDS 30.0%
GTAACTATAGGAATCCAAAA+TGG + chr7.2:29686687-29686706 None:intergenic 30.0%
! TTGCTCTTTTGAATGCAATA+CGG + chr7.2:29686659-29686678 None:intergenic 30.0%
!! CTACTCAATCCTTTTAAGTA+TGG - chr7.2:29686504-29686523 MS.gene27636:CDS 30.0%
!! TGTGATGGATGAAAATTGAA+TGG + chr7.2:29686418-29686437 None:intergenic 30.0%
!!! TCCTAAATTGATGGTTTTGA+TGG + chr7.2:29686308-29686327 None:intergenic 30.0%
AAGTTTGTCCAGCATAATCT+TGG + chr7.2:29686884-29686903 None:intergenic 35.0%
ACAGTGTTCCAGAAGATTTA+GGG - chr7.2:29687153-29687172 MS.gene27636:CDS 35.0%
AGAAGAACTTCCTCTTAGTT+TGG - chr7.2:29686200-29686219 MS.gene27636:CDS 35.0%
AGTTTGTCCAGCATAATCTT+GGG + chr7.2:29686883-29686902 None:intergenic 35.0%
ATAATCATCTGGGCTTATGA+TGG + chr7.2:29686610-29686629 None:intergenic 35.0%
ATTGAATGGAGACATATGGA+TGG - chr7.2:29686917-29686936 MS.gene27636:CDS 35.0%
CGACGTTTACAAATTGAAAC+TGG - chr7.2:29686983-29687002 MS.gene27636:CDS 35.0%
CTATAGGAATCCAAAATGGT+TGG + chr7.2:29686683-29686702 None:intergenic 35.0%
CTTTCATTAGTCCACAGTAA+CGG - chr7.2:29686471-29686490 MS.gene27636:CDS 35.0%
GACATCGAAAAGCTTTCATA+TGG - chr7.2:29686957-29686976 MS.gene27636:CDS 35.0%
GCTAGTTATCTTCTCATAGT+AGG + chr7.2:29686841-29686860 None:intergenic 35.0%
TAATCATCTGGGCTTATGAT+GGG + chr7.2:29686609-29686628 None:intergenic 35.0%
TTGTCAAGAACCAAACTAAG+AGG + chr7.2:29686213-29686232 None:intergenic 35.0%
TTTGAACTTGGAGAAACAAG+AGG + chr7.2:29687108-29687127 None:intergenic 35.0%
! AAAGAGCAAACCAACCATTT+TGG - chr7.2:29686670-29686689 MS.gene27636:CDS 35.0%
! ATTGAATGGAGTACTCTTTG+TGG + chr7.2:29686404-29686423 None:intergenic 35.0%
! GTTTGGGATCTATAATGTGA+AGG - chr7.2:29687175-29687194 MS.gene27636:CDS 35.0%
! TTCCAGAAGATTTAGGGTTT+GGG - chr7.2:29687159-29687178 MS.gene27636:CDS 35.0%
! TTGTACAAGTTTTCCACTCT+GGG + chr7.2:29687012-29687031 None:intergenic 35.0%
! TTTGTACAAGTTTTCCACTC+TGG + chr7.2:29687013-29687032 None:intergenic 35.0%
AAAAAATTGCGTCTGCTCGA+AGG + chr7.2:29687234-29687253 None:intergenic 40.0%
AATCATCTGGGCTTATGATG+GGG + chr7.2:29686608-29686627 None:intergenic 40.0%
ACACTCCGGAGATATCTTAA+AGG + chr7.2:29686570-29686589 None:intergenic 40.0%
ACGACAGCAACATAATCATC+TGG + chr7.2:29686621-29686640 None:intergenic 40.0%
AGGATTGAATAGTGTCTGAG+TGG + chr7.2:29686767-29686786 None:intergenic 40.0%
AGTACTCTTTGTGGCTTAGA+AGG + chr7.2:29686395-29686414 None:intergenic 40.0%
ATACAAGGATCTACTGCTCT+TGG + chr7.2:29686370-29686389 None:intergenic 40.0%
ATCCCAAACCCTAAATCTTC+TGG + chr7.2:29687164-29687183 None:intergenic 40.0%
CGACAGCAACATAATCATCT+GGG + chr7.2:29686620-29686639 None:intergenic 40.0%
GACAGTGTTCCAGAAGATTT+AGG - chr7.2:29687152-29687171 MS.gene27636:CDS 40.0%
GCTGTCGTCATTTACAACTT+TGG - chr7.2:29686633-29686652 MS.gene27636:CDS 40.0%
GGATTGAATAGTGTCTGAGT+GGG + chr7.2:29686766-29686785 None:intergenic 40.0%
GGCTCTACCTCAATTTCAAT+GGG - chr7.2:29687256-29687275 MS.gene27636:CDS 40.0%
GGGATCTATAATGTGAAGGA+TGG - chr7.2:29687179-29687198 MS.gene27636:CDS 40.0%
TACTGCTCTTGGATTCGATT+GGG + chr7.2:29686359-29686378 None:intergenic 40.0%
! GTTCCAGAAGATTTAGGGTT+TGG - chr7.2:29687158-29687177 MS.gene27636:CDS 40.0%
!! CACCACCTTTAAGATATCTC+CGG - chr7.2:29686562-29686581 MS.gene27636:CDS 40.0%
!!! CGAGCAGACGCAATTTTTTT+GGG - chr7.2:29687235-29687254 MS.gene27636:CDS 40.0%
!!! TCGAGCAGACGCAATTTTTT+TGG - chr7.2:29687234-29687253 MS.gene27636:CDS 40.0%
ACAAGAGGCAGACACAGAAA+AGG + chr7.2:29687093-29687112 None:intergenic 45.0%
ACCAAATGAAGAGCCATGAC+AGG + chr7.2:29686448-29686467 None:intergenic 45.0%
ACCTGTCATGGCTCTTCATT+TGG - chr7.2:29686444-29686463 MS.gene27636:CDS 45.0%
ATTGGGACGCTCATGATCAT+GGG + chr7.2:29686342-29686361 None:intergenic 45.0%
CTACTGCTCTTGGATTCGAT+TGG + chr7.2:29686360-29686379 None:intergenic 45.0%
CTCCGGAGATATCTTAAAGG+TGG + chr7.2:29686567-29686586 None:intergenic 45.0%
CTTAAAGGTGGTGATTCGAG+AGG + chr7.2:29686555-29686574 None:intergenic 45.0%
GAGTTATGGAACCGTTACTG+TGG + chr7.2:29686485-29686504 None:intergenic 45.0%
GGGCTCTACCTCAATTTCAA+TGG - chr7.2:29687255-29687274 MS.gene27636:CDS 45.0%
GTCATGGCTCTTCATTTGGT+TGG - chr7.2:29686448-29686467 MS.gene27636:CDS 45.0%
GTGGCTTAGAAGGCTATACA+AGG + chr7.2:29686385-29686404 None:intergenic 45.0%
TAGGGTCATTTGAGCGATCA+GGG + chr7.2:29686812-29686831 None:intergenic 45.0%
TTAGGGTCATTTGAGCGATC+AGG + chr7.2:29686813-29686832 None:intergenic 45.0%
!! AGGAGGAGTGTTGAACTTGA+AGG + chr7.2:29686787-29686806 None:intergenic 45.0%
AAAGGTGGTGATTCGAGAGG+TGG + chr7.2:29686552-29686571 None:intergenic 50.0%
AGGGTCATTTGAGCGATCAG+GGG + chr7.2:29686811-29686830 None:intergenic 50.0%
CGGCAGACAATGTAACACTC+CGG + chr7.2:29686584-29686603 None:intergenic 50.0%
GATTGGGACGCTCATGATCA+TGG + chr7.2:29686343-29686362 None:intergenic 50.0%
GCTAGAGCCCAAGATTATGC+TGG - chr7.2:29686873-29686892 MS.gene27636:CDS 50.0%
TCGAGAGGTGGCAGACTAAT+GGG + chr7.2:29686540-29686559 None:intergenic 50.0%
TCGGTTTCGTGTGCAAGACT+TGG - chr7.2:29686262-29686281 MS.gene27636:CDS 50.0%
TTCGAGAGGTGGCAGACTAA+TGG + chr7.2:29686541-29686560 None:intergenic 50.0%
TTGAAACTGGACACCCAGAG+TGG - chr7.2:29686996-29687015 MS.gene27636:CDS 50.0%
! ATCTGGGCTTATGATGGGGT+CGG + chr7.2:29686604-29686623 None:intergenic 50.0%
AGAGGTGGCAGACTAATGGG+AGG + chr7.2:29686537-29686556 None:intergenic 55.0%
GGGTCATTTGAGCGATCAGG+GGG + chr7.2:29686810-29686829 None:intergenic 55.0%
TCATTTGAGCGATCAGGGGG+AGG + chr7.2:29686807-29686826 None:intergenic 55.0%
TGCGTCTGCTCGAAGGAAAG+TGG + chr7.2:29687227-29687246 None:intergenic 55.0%
GGGACGCTCATGATCATGGG+TGG + chr7.2:29686339-29686358 None:intergenic 60.0%
TTTGAGCGATCAGGGGGAGG+AGG + chr7.2:29686804-29686823 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 29686171 29687283 29686171 ID=MS.gene27636
chr7.2 mRNA 29686171 29687283 29686171 ID=MS.gene27636.t1;Parent=MS.gene27636
chr7.2 exon 29686171 29687283 29686171 ID=MS.gene27636.t1.exon1;Parent=MS.gene27636.t1
chr7.2 CDS 29686171 29687283 29686171 ID=cds.MS.gene27636.t1;Parent=MS.gene27636.t1
Gene Sequence

>MS.gene27636

ATGAGGAAGGTGGTAGACTGGGCTAACTTAGAAGAACTTCCTCTTAGTTTGGTTCTTGACAAGTTAGAAGAACACATAGATCATCTTAGATTCGGTTTCGTGTGCAAGACTTGGCGATCAGTTGCAAAGCTTAACCATCAAAACCATCAATTTAGGAATACTATACCACCCATGATCATGAGCGTCCCAATCGAATCCAAGAGCAGTAGATCCTTGTATAGCCTTCTAAGCCACAAAGAGTACTCCATTCAATTTTCATCCATCACATTTATAACCTGTCATGGCTCTTCATTTGGTTGGCTTTCATTAGTCCACAGTAACGGTTCCATAACTCTACTCAATCCTTTTAAGTATGGTATTATTCCTCCCATTAGTCTGCCACCTCTCGAATCACCACCTTTAAGATATCTCCGGAGTGTTACATTGTCTGCCGACCCCATCATAAGCCCAGATGATTATGTTGCTGTCGTCATTTACAACTTTGGCCGTATTGCATTCAAAAGAGCAAACCAACCATTTTGGATTCCTATAGTTACAAATTTATTACATTTCAATGATGTTGTATTTTATAAAGGCATGTTAGTCGCTGAGACCCACTCAGACACTATTCAATCCTTCAAGTTCAACACTCCTCCTCCCCCTGATCGCTCAAATGACCCTAATTTTACCTACTATGAGAAGATAACTAGCACTGAATATCGTGCTAGAGCCCAAGATTATGCTGGACAAACTTATCTTGTGAAATCATTGAATGGAGACATATGGATGGTGAAAAGATATCTAATCGACATCGAAAAGCTTTCATATGGACTCGACGTTTACAAATTGAAACTGGACACCCAGAGTGGAAAACTTGTACAAATGAACAGACTTAAGAGTTTGGGAGACAACATTTTATTTATCGGATATTGTGGTAATTCCTTTTCTGTGTCTGCCTCTTGTTTCTCCAAGTTCAAAAAAGATTCAGTTTATTTTCTCTGTGACAGTGTTCCAGAAGATTTAGGGTTTGGGATCTATAATGTGAAGGATGGAAGCTATCAGATACAATCTTTACCACTTTCCTTCGAGCAGACGCAATTTTTTTGGGCTCTACCTCAATTTCAATGGGATTGA
Protein sequence

>MS.gene27636.t1

MRKVVDWANLEELPLSLVLDKLEEHIDHLRFGFVCKTWRSVAKLNHQNHQFRNTIPPMIMSVPIESKSSRSLYSLLSHKEYSIQFSSITFITCHGSSFGWLSLVHSNGSITLLNPFKYGIIPPISLPPLESPPLRYLRSVTLSADPIISPDDYVAVVIYNFGRIAFKRANQPFWIPIVTNLLHFNDVVFYKGMLVAETHSDTIQSFKFNTPPPPDRSNDPNFTYYEKITSTEYRARAQDYAGQTYLVKSLNGDIWMVKRYLIDIEKLSYGLDVYKLKLDTQSGKLVQMNRLKSLGDNILFIGYCGNSFSVSASCFSKFKKDSVYFLCDSVPEDLGFGIYNVKDGSYQIQSLPLSFEQTQFFWALPQFQWD