AlfalfaGEDB | MS.gene27753

M. sativa cultivar XinJiangDaYe / MS.gene27753

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27753.t1	AES80177.2	70.7	276	73	3	1	271	99	371	2.90E-99	371.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27753.t1	G7L4M9	70.7	276	73	3	1	271	99	371	2.1e-99	371.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene27753	MS.gene73460	PPI
MS.gene27753	MS.gene43191	PPI
MS.gene27753	MS.gene73726	PPI
MS.gene27753	MS.gene49592	PPI
MS.gene27753	MS.gene94239	PPI
MS.gene27753	MS.gene023892	PPI
MS.gene27753	MS.gene73723	PPI
MS.gene27753	MS.gene023785	PPI
MS.gene27753	MS.gene27637	PPI
MS.gene27753	MS.gene032784	PPI
MS.gene27753	MS.gene76061	PPI
MS.gene27753	MS.gene95450	PPI
MS.gene99024	MS.gene27753	PPI
MS.gene27753	MS.gene52262	PPI
MS.gene27753	MS.gene49594	PPI
MS.gene023891	MS.gene27753	PPI
MS.gene27753	MS.gene52253	PPI
MS.gene27753	MS.gene52255	PPI
MS.gene27753	MS.gene42431	PPI
MS.gene041287	MS.gene27753	PPI
MS.gene27745	MS.gene27753	PPI
MS.gene27753	MS.gene27639	PPI
MS.gene27753	MS.gene22627	PPI
MS.gene27753	MS.gene031136	PPI
MS.gene27753	MS.gene46208	PPI
MS.gene27753	MS.gene023787	PPI
MS.gene29651	MS.gene27753	PPI
MS.gene27753	MS.gene027042	PPI
MS.gene27753	MS.gene42432	PPI
MS.gene27753	MS.gene49593	PPI
MS.gene27753	MS.gene29654	PPI
MS.gene27753	MS.gene52163	PPI
MS.gene27753	MS.gene42429	PPI
MS.gene27753	MS.gene52913	PPI
MS.gene27753	MS.gene86085	PPI
MS.gene27753	MS.gene52261	PPI
MS.gene032780	MS.gene27753	PPI
MS.gene066291	MS.gene27753	PPI
MS.gene27753	MS.gene031137	PPI
MS.gene27753	MS.gene99022	PPI
MS.gene27753	MS.gene27745	PPI
MS.gene029514	MS.gene27753	PPI
MS.gene022086	MS.gene27753	PPI
MS.gene27753	MS.gene42226	PPI
MS.gene27749	MS.gene27753	PPI
MS.gene27753	MS.gene94237	PPI
MS.gene27753	MS.gene52263	PPI
MS.gene27753	MS.gene76056	PPI
MS.gene27753	MS.gene52166	PPI
MS.gene27753	MS.gene52164	PPI
MS.gene27753	MS.gene023887	PPI
MS.gene42430	MS.gene27753	PPI
MS.gene27753	MS.gene76059	PPI
MS.gene27753	MS.gene27636	PPI
MS.gene27753	MS.gene85835	PPI
MS.gene27750	MS.gene27753	PPI
MS.gene27753	MS.gene29651	PPI
MS.gene27753	MS.gene032785	PPI
MS.gene27753	MS.gene42433	PPI
MS.gene52697	MS.gene27753	PPI
MS.gene27753	MS.gene023893	PPI
MS.gene27753	MS.gene27754	PPI
MS.gene27753	MS.gene27635	PPI
MS.gene27753	MS.gene035135	PPI
MS.gene27753	MS.gene27748	PPI
MS.gene27753	MS.gene27751	PPI
MS.gene066292	MS.gene27753	PPI
MS.gene023889	MS.gene27753	PPI
MS.gene27753	MS.gene42223	PPI
MS.gene27753	MS.gene42428	PPI
MS.gene066289	MS.gene27753	PPI
MS.gene27753	MS.gene023784	PPI
MS.gene52258	MS.gene27753	PPI
MS.gene27753	MS.gene52254	PPI
MS.gene52163	MS.gene27753	PPI
MS.gene27753	MS.gene85826	PPI
MS.gene27753	MS.gene023890	PPI
MS.gene42222	MS.gene27753	PPI
MS.gene27753	MS.gene023888	PPI
MS.gene27753	MS.gene99023	PPI
MS.gene27753	MS.gene52259	PPI
MS.gene27753	MS.gene035137	PPI
MS.gene27753	MS.gene97317	PPI
MS.gene27753	MS.gene44542	PPI
MS.gene27753	MS.gene27747	PPI
MS.gene27753	MS.gene42224	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27753.t1	MTR_7g077650	70.652	276	73	3	1	271	99	371	5.12e-129	371
MS.gene27753.t1	MTR_7g077660	70.956	272	74	4	2	271	99	367	4.22e-126	363
MS.gene27753.t1	MTR_7g077620	68.889	270	79	4	2	268	101	368	5.37e-125	361
MS.gene27753.t1	MTR_7g076110	53.986	276	116	6	6	271	38	312	1.54e-90	271
MS.gene27753.t1	MTR_7g076100	60.504	238	85	6	39	271	66	299	2.71e-88	265
MS.gene27753.t1	MTR_1g108740	45.387	271	119	12	6	270	97	344	4.08e-58	189
MS.gene27753.t1	MTR_5g038260	41.776	304	132	12	2	272	92	383	1.82e-53	178
MS.gene27753.t1	MTR_5g038230	41.971	274	98	10	2	248	90	329	1.74e-48	164
MS.gene27753.t1	MTR_5g082770	44.186	215	91	5	6	206	90	289	2.03e-42	147
MS.gene27753.t1	MTR_7g076130	56.800	125	50	2	69	190	25	148	6.94e-41	139
MS.gene27753.t1	MTR_1g064570	36.000	325	138	14	2	271	90	399	1.46e-39	142
MS.gene27753.t1	MTR_5g082780	37.339	233	114	8	15	234	1	214	3.66e-37	131
MS.gene27753.t1	MTR_1g016150	48.276	87	41	3	6	89	106	191	4.05e-16	75.9
MS.gene27753.t1	MTR_6g023955	41.837	98	53	3	41	137	73	167	2.90e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 46 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGAGTGGATGCATCATTTC+TGG	0.085063	7.3:-29145924	MS.gene27753:CDS
GATCATATGGATTCACTTTC+AGG	0.127702	7.3:-29146006	MS.gene27753:CDS
AGGAGGATTGTTTAGCTTTA+AGG	0.194494	7.3:+29146365	None:intergenic
AAAGAGCAAGCCAATCATTT+TGG	0.202770	7.3:-29146479	MS.gene27753:CDS
AATGAACAAACTTGAGAGTT+TGG	0.291432	7.3:-29146124	MS.gene27753:CDS
TCGAGAGGTGGCAGACTAAT+AGG	0.314526	7.3:+29146597	None:intergenic
GGAGGAATACTACACTTAAA+AGG	0.326804	7.3:+29146618	None:intergenic
ACCTCTCGAATCTCTCAAAA+AGG	0.330053	7.3:-29146583	MS.gene27753:CDS
CCATCATAACAACGTAGTTT+TGG	0.331154	7.3:+29146276	None:intergenic
CGAGTGGATGCATCATTTCT+GGG	0.345780	7.3:-29145923	MS.gene27753:CDS
GATTTATATGAATGATCATA+TGG	0.372503	7.3:-29146019	MS.gene27753:CDS
CCATGGTTGGCTTGCATTGG+TGG	0.397590	7.3:-29146670	MS.gene27753:CDS
GGTTCTGCCACAATGTCAAT+GGG	0.404513	7.3:-29145902	MS.gene27753:CDS
CCAAAACTACGTTGTTATGA+TGG	0.407066	7.3:-29146276	MS.gene27753:CDS
GGGTTCTGCCACAATGTCAA+TGG	0.415171	7.3:-29145903	MS.gene27753:CDS
TTGTTTCTCCACATTGGAAA+AGG	0.424044	7.3:-29146046	MS.gene27753:CDS
ACTAGCCATCTTCTCATAGT+AGG	0.443005	7.3:+29146314	None:intergenic
ATCATATGGATTCACTTTCA+GGG	0.459930	7.3:-29146005	MS.gene27753:CDS
TAGCCATGGTTGGCTTGCAT+TGG	0.461010	7.3:-29146673	MS.gene27753:CDS
AGCCTCTTGTTTCTCCACAT+TGG	0.483410	7.3:-29146052	MS.gene27753:CDS
TAATCACTTGGATTTGTCAT+GGG	0.490766	7.3:+29146543	None:intergenic
AGAATCTATAATGTGAAGGA+TGG	0.493671	7.3:-29145979	MS.gene27753:CDS
CTACGCGAATCCAAAATGAT+TGG	0.502017	7.3:+29146469	None:intergenic
TCTTACCGCTTTCCTTCGAG+TGG	0.511644	7.3:-29145939	MS.gene27753:CDS
TGAGAAGATGCTTAATCGAC+GGG	0.520399	7.3:-29146209	MS.gene27753:CDS
AATCATTGACCGGAGACATA+TGG	0.521122	7.3:-29146236	MS.gene27753:CDS
AATGATTTCACAAAATAAGC+AGG	0.533478	7.3:+29146252	None:intergenic
ATAATCACTTGGATTTGTCA+TGG	0.537229	7.3:+29146542	None:intergenic
TGTCATGGGGTCAGCAGACA+AGG	0.556574	7.3:+29146557	None:intergenic
TCTCATCAACCATATGTCTC+CGG	0.558876	7.3:+29146227	None:intergenic
ATGAGAAGATGCTTAATCGA+CGG	0.569894	7.3:-29146210	MS.gene27753:CDS
GACAAATCCAAGTGATTATG+TGG	0.574598	7.3:-29146538	MS.gene27753:CDS
TTGGAACTGAATGCCGAGAG+TGG	0.585480	7.3:-29146159	MS.gene27753:CDS
CGATCAATTGAGTCATCAGA+AGG	0.588157	7.3:+29146345	None:intergenic
GTTTAGCTTTAAGGATACAA+TGG	0.589562	7.3:+29146374	None:intergenic
GCTGCAACCACATAATCACT+TGG	0.591162	7.3:+29146531	None:intergenic
AATCACTTGGATTTGTCATG+GGG	0.596697	7.3:+29146544	None:intergenic
GTTAAGAATCTATAATGTGA+AGG	0.604544	7.3:-29145983	MS.gene27753:CDS
GAAATGATGCATCCACTCGA+AGG	0.618187	7.3:+29145927	None:intergenic
GATCAACCCCATTGACATTG+TGG	0.628960	7.3:+29145895	None:intergenic
TTCCAATGTGGAGAAACAAG+AGG	0.648839	7.3:+29146050	None:intergenic
TGCATCCACTCGAAGGAAAG+CGG	0.655155	7.3:+29145934	None:intergenic
TCAATTGAGTCATCAGAAGG+AGG	0.655171	7.3:+29146348	None:intergenic
CCACCAATGCAAGCCAACCA+TGG	0.665097	7.3:+29146670	None:intergenic
AGAGGTGGCAGACTAATAGG+AGG	0.669617	7.3:+29146600	None:intergenic
GTTCTGCCACAATGTCAATG+GGG	0.759112	7.3:-29145901	MS.gene27753:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATTTATATGAATGATCATA+TGG	-	chr7.3:29146571-29146590	MS.gene27753:CDS	20.0%
!!!	ACGAATATTGTGTTTTATAA+AGG	-	chr7.3:29146164-29146183	MS.gene27753:CDS	20.0%
!	AATGATTTCACAAAATAAGC+AGG	+	chr7.3:29146341-29146360	None:intergenic	25.0%
!	GTTAAGAATCTATAATGTGA+AGG	-	chr7.3:29146607-29146626	MS.gene27753:CDS	25.0%
!!	TATTGTGTTTTATAAAGGCT+TGG	-	chr7.3:29146169-29146188	MS.gene27753:CDS	25.0%
!!	TATTTTGTGAAATCATTGAC+CGG	-	chr7.3:29146344-29146363	MS.gene27753:CDS	25.0%
!!!	ATTTTATTTGTTGGAAGTGT+TGG	-	chr7.3:29146497-29146516	MS.gene27753:CDS	25.0%
!!!	GAAGACAACATTTTATTTGT+TGG	-	chr7.3:29146488-29146507	MS.gene27753:CDS	25.0%
	AATGAACAAACTTGAGAGTT+TGG	-	chr7.3:29146466-29146485	MS.gene27753:CDS	30.0%
	AGAATCTATAATGTGAAGGA+TGG	-	chr7.3:29146611-29146630	MS.gene27753:CDS	30.0%
	ATAATCACTTGGATTTGTCA+TGG	+	chr7.3:29146051-29146070	None:intergenic	30.0%
	ATCATATGGATTCACTTTCA+GGG	-	chr7.3:29146585-29146604	MS.gene27753:CDS	30.0%
	TAATCACTTGGATTTGTCAT+GGG	+	chr7.3:29146050-29146069	None:intergenic	30.0%
!	CAAAATGCACGTTTTCAAAT+TGG	-	chr7.3:29146412-29146431	MS.gene27753:CDS	30.0%
!	TTTTTCCTACTATGAGAAGA+TGG	-	chr7.3:29146271-29146290	MS.gene27753:CDS	30.0%
!!	GTTTAGCTTTAAGGATACAA+TGG	+	chr7.3:29146219-29146238	None:intergenic	30.0%
!!!	CGTAGTTTTGGAATCATATA+TGG	+	chr7.3:29146305-29146324	None:intergenic	30.0%
	AATCACTTGGATTTGTCATG+GGG	+	chr7.3:29146049-29146068	None:intergenic	35.0%
	ATGAGAAGATGCTTAATCGA+CGG	-	chr7.3:29146380-29146399	MS.gene27753:CDS	35.0%
	CCAAAACTACGTTGTTATGA+TGG	-	chr7.3:29146314-29146333	MS.gene27753:CDS	35.0%
	GACAAATCCAAGTGATTATG+TGG	-	chr7.3:29146052-29146071	MS.gene27753:CDS	35.0%
	GATCATATGGATTCACTTTC+AGG	-	chr7.3:29146584-29146603	MS.gene27753:CDS	35.0%
	GGAGGAATACTACACTTAAA+AGG	+	chr7.3:29145975-29145994	None:intergenic	35.0%
!	AAAGAGCAAGCCAATCATTT+TGG	-	chr7.3:29146111-29146130	MS.gene27753:CDS	35.0%
!	AATCGAATCCTTTTCCAATG+TGG	+	chr7.3:29146555-29146574	None:intergenic	35.0%
!	CCATCATAACAACGTAGTTT+TGG	+	chr7.3:29146317-29146336	None:intergenic	35.0%
!	TTGTTTCTCCACATTGGAAA+AGG	-	chr7.3:29146544-29146563	MS.gene27753:CDS	35.0%
!!	AGGAGGATTGTTTAGCTTTA+AGG	+	chr7.3:29146228-29146247	None:intergenic	35.0%
!!	TTGTTCAAGTTTTCCACTCT+CGG	+	chr7.3:29146447-29146466	None:intergenic	35.0%
	AATCATTGACCGGAGACATA+TGG	-	chr7.3:29146354-29146373	MS.gene27753:CDS	40.0%
	ACCTCTCGAATCTCTCAAAA+AGG	-	chr7.3:29146007-29146026	MS.gene27753:CDS	40.0%
	ACTAGCCATCTTCTCATAGT+AGG	+	chr7.3:29146279-29146298	None:intergenic	40.0%
	CGATCAATTGAGTCATCAGA+AGG	+	chr7.3:29146248-29146267	None:intergenic	40.0%
	CTACGCGAATCCAAAATGAT+TGG	+	chr7.3:29146124-29146143	None:intergenic	40.0%
	TCAATTGAGTCATCAGAAGG+AGG	+	chr7.3:29146245-29146264	None:intergenic	40.0%
	TCTCATCAACCATATGTCTC+CGG	+	chr7.3:29146366-29146385	None:intergenic	40.0%
	TGAGAAGATGCTTAATCGAC+GGG	-	chr7.3:29146381-29146400	MS.gene27753:CDS	40.0%
	TTCCAATGTGGAGAAACAAG+AGG	+	chr7.3:29146543-29146562	None:intergenic	40.0%
!!	ACCTTTTTGAGAGATTCGAG+AGG	+	chr7.3:29146011-29146030	None:intergenic	40.0%
!!	TTTTTGAGAGATTCGAGAGG+TGG	+	chr7.3:29146008-29146027	None:intergenic	40.0%
	CGAGTGGATGCATCATTTCT+GGG	-	chr7.3:29146667-29146686	MS.gene27753:CDS	45.0%
	GAAATGATGCATCCACTCGA+AGG	+	chr7.3:29146666-29146685	None:intergenic	45.0%
	GCTGCAACCACATAATCACT+TGG	+	chr7.3:29146062-29146081	None:intergenic	45.0%
	GGTTCTGCCACAATGTCAAT+GGG	-	chr7.3:29146688-29146707	MS.gene27753:CDS	45.0%
	GTTCTGCCACAATGTCAATG+GGG	-	chr7.3:29146689-29146708	MS.gene27753:CDS	45.0%
	TCGAGTGGATGCATCATTTC+TGG	-	chr7.3:29146666-29146685	MS.gene27753:CDS	45.0%
!	AGCCTCTTGTTTCTCCACAT+TGG	-	chr7.3:29146538-29146557	MS.gene27753:CDS	45.0%
!!!	TTGTTTTTGGGCTGTAGCCA+TGG	-	chr7.3:29145903-29145922	MS.gene27753:CDS	45.0%
	AGAGGTGGCAGACTAATAGG+AGG	+	chr7.3:29145993-29146012	None:intergenic	50.0%
	GGGTTCTGCCACAATGTCAA+TGG	-	chr7.3:29146687-29146706	MS.gene27753:CDS	50.0%
	TCGAGAGGTGGCAGACTAAT+AGG	+	chr7.3:29145996-29146015	None:intergenic	50.0%
	TCTTACCGCTTTCCTTCGAG+TGG	-	chr7.3:29146651-29146670	MS.gene27753:CDS	50.0%
	TGCATCCACTCGAAGGAAAG+CGG	+	chr7.3:29146659-29146678	None:intergenic	50.0%
	TTGGAACTGAATGCCGAGAG+TGG	-	chr7.3:29146431-29146450	MS.gene27753:CDS	50.0%
!	TTTTGGGCTGTAGCCATGGT+TGG	-	chr7.3:29145907-29145926	MS.gene27753:CDS	50.0%
!!	TAGCCATGGTTGGCTTGCAT+TGG	-	chr7.3:29145917-29145936	MS.gene27753:CDS	50.0%
	CCACCAATGCAAGCCAACCA+TGG	+	chr7.3:29145923-29145942	None:intergenic	55.0%
	TGTCATGGGGTCAGCAGACA+AGG	+	chr7.3:29146036-29146055	None:intergenic	55.0%
!!	CCATGGTTGGCTTGCATTGG+TGG	-	chr7.3:29145920-29145939	MS.gene27753:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	29145897	29146715	29145897	ID=MS.gene27753
chr7.3	mRNA	29145897	29146715	29145897	ID=MS.gene27753.t1;Parent=MS.gene27753
chr7.3	exon	29145897	29146715	29145897	ID=MS.gene27753.t1.exon1;Parent=MS.gene27753.t1
chr7.3	CDS	29145897	29146715	29145897	ID=cds.MS.gene27753.t1;Parent=MS.gene27753.t1

Gene Sequence

>MS.gene27753

ATGAAATTGTTTTTGGGCTGTAGCCATGGTTGGCTTGCATTGGTGGATGATAACGATGTCATTACTCTAGTGAATCCTTTTAAGTGTAGTATTCCTCCTATTAGTCTGCCACCTCTCGAATCTCTCAAAAAGGTTACCTTGTCTGCTGACCCCATGACAAATCCAAGTGATTATGTGGTTGCAGCAATTCACAAGTTTGTTTATCTCGCTTTCAAAAGAGCAAGCCAATCATTTTGGATTCGCGTAGACACAAATGAATTTACTTTCACGAATATTGTGTTTTATAAAGGCTTGGTATTTGCTGATAGCAACAGAGACACCATTGTATCCTTAAAGCTAAACAATCCTCCTTCTGATGACTCAATTGATCGTAATTTTTCCTACTATGAGAAGATGGCTAGTACTCCATATATGATTCCAAAACTACGTTGTTATGATGGACCTGCTTATTTTGTGAAATCATTGACCGGAGACATATGGTTGATGAGAAGATGCTTAATCGACGGGTTTTCTATCAAAATGCACGTTTTCAAATTGGAACTGAATGCCGAGAGTGGAAAACTTGAACAAATGAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTTGGAAGTGTTGGTGATTCAATTTCTGTGTCAGCCTCTTGTTTCTCCACATTGGAAAAGGATTCGATTTATATGAATGATCATATGGATTCACTTTCAGGGTTAAGAATCTATAATGTGAAGGATGGAAGTTATCGCAATCAATTCTTACCGCTTTCCTTCGAGTGGATGCATCATTTCTGGGTTCTGCCACAATGTCAATGGGGTTGA

Protein sequence

>MS.gene27753.t1

MKLFLGCSHGWLALVDDNDVITLVNPFKCSIPPISLPPLESLKKVTLSADPMTNPSDYVVAAIHKFVYLAFKRASQSFWIRVDTNEFTFTNIVFYKGLVFADSNRDTIVSLKLNNPPSDDSIDRNFSYYEKMASTPYMIPKLRCYDGPAYFVKSLTGDIWLMRRCLIDGFSIKMHVFKLELNAESGKLEQMNKLESLEDNILFVGSVGDSISVSASCFSTLEKDSIYMNDHMDSLSGLRIYNVKDGSYRNQFLPLSFEWMHHFWVLPQCQWG