AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49594

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49594.t1 MTR_5g038260 92.388 381 28 1 1 380 3 383 0.0 738
MS.gene49594.t1 MTR_5g038230 84.474 380 34 2 1 380 1 355 0.0 658
MS.gene49594.t1 MTR_1g064570 49.876 403 176 9 1 380 1 400 6.05e-131 381
MS.gene49594.t1 MTR_1g108740 44.909 383 164 10 2 378 3 344 2.86e-98 296
MS.gene49594.t1 MTR_5g082770 50.654 306 131 10 1 303 1 289 1.29e-86 264
MS.gene49594.t1 MTR_7g077620 42.239 393 182 15 4 378 5 370 4.64e-78 246
MS.gene49594.t1 MTR_7g077660 41.058 397 181 17 4 380 5 368 1.48e-73 233
MS.gene49594.t1 MTR_7g077650 40.247 405 177 18 4 380 5 372 1.32e-72 231
MS.gene49594.t1 MTR_5g082780 44.269 253 118 8 103 351 1 234 3.05e-54 179
MS.gene49594.t1 MTR_7g076110 36.825 315 166 12 77 380 21 313 3.51e-45 158
MS.gene49594.t1 MTR_1g016150 44.974 189 79 7 2 177 3 179 5.60e-45 155
MS.gene49594.t1 MTR_1g016150 55.072 69 24 1 248 316 175 236 7.14e-14 70.9
MS.gene49594.t1 MTR_7g076100 41.732 254 114 11 139 380 69 300 4.22e-39 141
MS.gene49594.t1 MTR_7g076100 60.000 50 19 1 4 53 6 54 3.50e-11 63.9
MS.gene49594.t1 MTR_1g108750 43.750 160 51 5 1 157 1 124 6.44e-26 101
MS.gene49594.t1 MTR_6g023955 48.673 113 50 2 112 217 40 151 1.05e-24 100
MS.gene49594.t1 MTR_6g023955 57.377 61 26 0 319 379 149 209 5.84e-18 82.0
MS.gene49594.t1 MTR_3g114990 26.139 417 237 17 1 375 20 407 8.74e-20 90.5
MS.gene49594.t1 MTR_3g114970 25.000 392 237 16 1 375 1 352 1.18e-17 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTATATTGCTCCTAAAT+TGG 0.217987 5.1:-28844120 None:intergenic
TAATCATTTGGACTTGTTAT+AGG 0.239358 5.1:-28844418 None:intergenic
AATAGTAAATTGAATCCTTT+TGG 0.242301 5.1:-28844930 None:intergenic
TGGTGAGAAGGTTTATAAAT+AGG 0.247820 5.1:+28844734 MS.gene49594:CDS
ATACAAGACACTGACCATTT+TGG 0.281472 5.1:+28844514 MS.gene49594:CDS
TAAAAGCAGGACAAGAGTTT+TGG 0.281524 5.1:+28844485 MS.gene49594:CDS
CTTCTTTGTAACAAAACATT+TGG 0.292647 5.1:-28844649 None:intergenic
TTAAGCATTGTCAATTCTAA+TGG 0.313392 5.1:-28844217 None:intergenic
TTGTTATTCAAGTGACCCTT+GGG 0.317511 5.1:+28844612 MS.gene49594:CDS
ATAGCAGCAACATCCTTAAA+AGG 0.324907 5.1:-28844316 None:intergenic
TTGTCGAAACTTGAGAGTTT+AGG 0.326449 5.1:+28844838 MS.gene49594:CDS
GCCACCCATCGATAATTATA+TGG 0.327281 5.1:+28844345 MS.gene49594:CDS
ATCAACACGATCACCAATTT+AGG 0.336169 5.1:+28844107 MS.gene49594:CDS
TTGAACCAATTGATCACATT+TGG 0.344806 5.1:+28844038 MS.gene49594:CDS
CCAATTGATCACATTTGGTT+TGG 0.352054 5.1:+28844043 MS.gene49594:CDS
TTCTTTACATGTATTTAAGT+TGG 0.365055 5.1:+28844783 MS.gene49594:CDS
GCATAACCATGGAGCGAGAT+TGG 0.373158 5.1:+28843969 None:intergenic
CCAAACCAAATGTGATCAAT+TGG 0.396537 5.1:-28844043 None:intergenic
GGTGAGAAGGTTTATAAATA+GGG 0.399787 5.1:+28844735 MS.gene49594:CDS
TACTTATTTAGTGAAATCAT+TGG 0.416138 5.1:+28844696 MS.gene49594:CDS
CATTACATTCTACAATGGTT+TGG 0.419262 5.1:+28844546 MS.gene49594:CDS
GCTGCAACCACATAATCATT+TGG 0.420868 5.1:-28844430 None:intergenic
TAATGTCGATGAAACCAAAA+TGG 0.420892 5.1:-28844528 None:intergenic
CGATAATTATATGGTATGTA+AGG 0.424343 5.1:+28844354 MS.gene49594:CDS
AAAAGGTGGCACAACCCATA+TGG 0.432547 5.1:-28845085 None:intergenic
AAGGTATGACACCTCCCATA+TGG 0.446486 5.1:+28845070 MS.gene49594:CDS
CATAACCATGGAGCGAGATT+GGG 0.451858 5.1:+28843970 None:intergenic
TCAAAAGGACCTCTAGGATA+AGG 0.452966 5.1:-28844982 None:intergenic
CCTAATCAATTCCATTGAAA+AGG 0.460995 5.1:-28845102 None:intergenic
GAGAGTTGTGGATGGTGAGA+AGG 0.464771 5.1:+28844722 MS.gene49594:CDS
CGTTACAAATCTTACTTCAA+AGG 0.467196 5.1:+28845051 MS.gene49594:CDS
TTTGTTATTCAAGTGACCCT+TGG 0.473541 5.1:+28844611 MS.gene49594:CDS
TTTCTCAAGTTACCTCCAAA+AGG 0.480419 5.1:+28844915 MS.gene49594:CDS
TGTGCATAAGGTTACATTGT+CGG 0.510731 5.1:+28844390 MS.gene49594:CDS
TTCAAGTGACCCTTGGGGTG+AGG 0.511989 5.1:+28844618 MS.gene49594:CDS
AACAAGTCCAAATGATTATG+TGG 0.518480 5.1:+28844423 MS.gene49594:CDS
AGGTATGACACCTCCCATAT+GGG 0.519021 5.1:+28845071 MS.gene49594:CDS
ACTTGAGCCACAACATCTCT+TGG 0.521360 5.1:-28844242 None:intergenic
TTGTAGATTCCCATATCAAA+AGG 0.521611 5.1:-28844997 None:intergenic
GTTGTGGCTCAAGTCATGGC+TGG 0.535182 5.1:+28844251 MS.gene49594:CDS
GATTTCACTAAATAAGTAAG+TGG 0.538843 5.1:-28844691 None:intergenic
AAAGGTGGCACAACCCATAT+GGG 0.541900 5.1:-28845084 None:intergenic
TGTCTAGCTTTCATAAAAGC+AGG 0.542520 5.1:+28844472 MS.gene49594:CDS
TTACGACTACAATGTGCATA+AGG 0.543562 5.1:+28844378 MS.gene49594:CDS
CTTTGAAGTAAGATTTGTAA+CGG 0.546410 5.1:-28845050 None:intergenic
TATTTAGTGAAATCATTGGA+GGG 0.549492 5.1:+28844700 MS.gene49594:CDS
ACATACCATATAATTATCGA+TGG 0.552414 5.1:-28844350 None:intergenic
GGACCTCTAGGATAAGGAAT+CGG 0.552728 5.1:-28844976 None:intergenic
AAGTTGGAATTGAATGACAA+AGG 0.553747 5.1:+28844799 MS.gene49594:CDS
GTTTGCCCAATCTCGCTCCA+TGG 0.556567 5.1:-28843975 None:intergenic
CCCATATCAAAAGGACCTCT+AGG 0.561580 5.1:-28844988 None:intergenic
TTATTTAGTGAAATCATTGG+AGG 0.564804 5.1:+28844699 MS.gene49594:CDS
GGAATTCTTTCCTCACCCCA+AGG 0.565481 5.1:-28844628 None:intergenic
ACGAAGAAGATGTCATCACT+TGG 0.566470 5.1:+28844287 MS.gene49594:CDS
GGAATCTACAACGTAAAGCA+TGG 0.570743 5.1:+28845009 MS.gene49594:CDS
AATCAATTCCATTGAAAAGG+TGG 0.576430 5.1:-28845099 None:intergenic
TCTGAAGGGATCATTAGCAT+AGG 0.577323 5.1:-28844142 None:intergenic
ATCGACATTACATTCTACAA+TGG 0.582494 5.1:+28844541 MS.gene49594:CDS
GAATTCTTTCCTCACCCCAA+GGG 0.583805 5.1:-28844627 None:intergenic
CTTAACACCAAGAGATGTTG+TGG 0.590075 5.1:+28844235 MS.gene49594:CDS
GGTGATCGTGTTGATGACTA+AGG 0.595842 5.1:-28844099 None:intergenic
AATCATTGGAGGGAGAGTTG+TGG 0.606251 5.1:+28844710 MS.gene49594:CDS
TGGTGTATGCTGTAACTCGT+TGG 0.616114 5.1:+28844566 MS.gene49594:CDS
CGAAGAAGATGTCATCACTT+GGG 0.623448 5.1:+28844288 MS.gene49594:CDS
ACCATATAATTATCGATGGG+TGG 0.627867 5.1:-28844346 None:intergenic
AGATGTTGTGGCTCAAGTCA+TGG 0.635745 5.1:+28844247 MS.gene49594:CDS
TTATGAAAGCTAGACAGCCA+CGG 0.638641 5.1:-28844465 None:intergenic
GAACCGATTCCTTATCCTAG+AGG 0.645299 5.1:+28844973 MS.gene49594:CDS
CATACCATATAATTATCGAT+GGG 0.648701 5.1:-28844349 None:intergenic
TGTTATTCAAGTGACCCTTG+GGG 0.675329 5.1:+28844613 MS.gene49594:CDS
GCAGCAATTTACACTGACCG+TGG 0.685734 5.1:+28844448 MS.gene49594:CDS
ATTGGAGGGAGAGTTGTGGA+TGG 0.690403 5.1:+28844714 MS.gene49594:CDS
GGTGGCACAACCCATATGGG+AGG 0.700789 5.1:-28845081 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTATTTAGTGAAATCAT+TGG + chr5.1:28844696-28844715 MS.gene49594:CDS 20.0%
!! TTCTTTACATGTATTTAAGT+TGG + chr5.1:28844783-28844802 MS.gene49594:CDS 20.0%
!!! AATAGTAAATTGAATCCTTT+TGG - chr5.1:28844933-28844952 None:intergenic 20.0%
!!! AATATTTTATTTGTAGGTGA+CGG + chr5.1:28844865-28844884 MS.gene49594:CDS 20.0%
! ACATACCATATAATTATCGA+TGG - chr5.1:28844353-28844372 None:intergenic 25.0%
! CATACCATATAATTATCGAT+GGG - chr5.1:28844352-28844371 None:intergenic 25.0%
! CGATAATTATATGGTATGTA+AGG + chr5.1:28844354-28844373 MS.gene49594:CDS 25.0%
! CTTCTTTGTAACAAAACATT+TGG - chr5.1:28844652-28844671 None:intergenic 25.0%
! GATTTCACTAAATAAGTAAG+TGG - chr5.1:28844694-28844713 None:intergenic 25.0%
! TATTTAGTGAAATCATTGGA+GGG + chr5.1:28844700-28844719 MS.gene49594:CDS 25.0%
! TTATTTAGTGAAATCATTGG+AGG + chr5.1:28844699-28844718 MS.gene49594:CDS 25.0%
!! TTAAGCATTGTCAATTCTAA+TGG - chr5.1:28844220-28844239 None:intergenic 25.0%
!!! CTTTGAAGTAAGATTTGTAA+CGG - chr5.1:28845053-28845072 None:intergenic 25.0%
!!! GGAGACAATATTTTATTTGT+AGG + chr5.1:28844859-28844878 MS.gene49594:CDS 25.0%
!!! TAATCATTTGGACTTGTTAT+AGG - chr5.1:28844421-28844440 None:intergenic 25.0%
AACAAGTCCAAATGATTATG+TGG + chr5.1:28844423-28844442 MS.gene49594:CDS 30.0%
AAGTTGGAATTGAATGACAA+AGG + chr5.1:28844799-28844818 MS.gene49594:CDS 30.0%
AGGGTCAAAAATATCAACAA+AGG + chr5.1:28844754-28844773 MS.gene49594:CDS 30.0%
ATCGACATTACATTCTACAA+TGG + chr5.1:28844541-28844560 MS.gene49594:CDS 30.0%
CATTACATTCTACAATGGTT+TGG + chr5.1:28844546-28844565 MS.gene49594:CDS 30.0%
CGTTACAAATCTTACTTCAA+AGG + chr5.1:28845051-28845070 MS.gene49594:CDS 30.0%
GCATTATATTGCTCCTAAAT+TGG - chr5.1:28844123-28844142 None:intergenic 30.0%
GGTGAGAAGGTTTATAAATA+GGG + chr5.1:28844735-28844754 MS.gene49594:CDS 30.0%
TAATGTCGATGAAACCAAAA+TGG - chr5.1:28844531-28844550 None:intergenic 30.0%
TGGTGAGAAGGTTTATAAAT+AGG + chr5.1:28844734-28844753 MS.gene49594:CDS 30.0%
TTGAACCAATTGATCACATT+TGG + chr5.1:28844038-28844057 MS.gene49594:CDS 30.0%
TTGTAGATTCCCATATCAAA+AGG - chr5.1:28845000-28845019 None:intergenic 30.0%
! AATGGATACACTCTATTTTC+AGG - chr5.1:28844202-28844221 None:intergenic 30.0%
! AGTAAATTGAATCCTTTTGG+AGG - chr5.1:28844930-28844949 None:intergenic 30.0%
ACCATATAATTATCGATGGG+TGG - chr5.1:28844349-28844368 None:intergenic 35.0%
ATAGCAGCAACATCCTTAAA+AGG - chr5.1:28844319-28844338 None:intergenic 35.0%
ATCAACACGATCACCAATTT+AGG + chr5.1:28844107-28844126 MS.gene49594:CDS 35.0%
CCAAACCAAATGTGATCAAT+TGG - chr5.1:28844046-28844065 None:intergenic 35.0%
CCAATTGATCACATTTGGTT+TGG + chr5.1:28844043-28844062 MS.gene49594:CDS 35.0%
TGTGCATAAGGTTACATTGT+CGG + chr5.1:28844390-28844409 MS.gene49594:CDS 35.0%
TTACGACTACAATGTGCATA+AGG + chr5.1:28844378-28844397 MS.gene49594:CDS 35.0%
TTGTCGAAACTTGAGAGTTT+AGG + chr5.1:28844838-28844857 MS.gene49594:CDS 35.0%
TTTCTCAAGTTACCTCCAAA+AGG + chr5.1:28844915-28844934 MS.gene49594:CDS 35.0%
! ATACAAGACACTGACCATTT+TGG + chr5.1:28844514-28844533 MS.gene49594:CDS 35.0%
! TAAAAGCAGGACAAGAGTTT+TGG + chr5.1:28844485-28844504 MS.gene49594:CDS 35.0%
! TTGTTATTCAAGTGACCCTT+GGG + chr5.1:28844612-28844631 MS.gene49594:CDS 35.0%
! TTTCTGGACTCTTTTCTGAA+GGG - chr5.1:28844159-28844178 None:intergenic 35.0%
! TTTTCTGGACTCTTTTCTGA+AGG - chr5.1:28844160-28844179 None:intergenic 35.0%
!! CTATACAAACATCGCTTTTC+TGG - chr5.1:28844175-28844194 None:intergenic 35.0%
!! TGTCTAGCTTTCATAAAAGC+AGG + chr5.1:28844472-28844491 MS.gene49594:CDS 35.0%
!! TTGGTTCTGTCTGCAAAAAT+TGG + chr5.1:28844062-28844081 MS.gene49594:CDS 35.0%
!! TTTGTTATTCAAGTGACCCT+TGG + chr5.1:28844611-28844630 MS.gene49594:CDS 35.0%
ACGAAGAAGATGTCATCACT+TGG + chr5.1:28844287-28844306 MS.gene49594:CDS 40.0%
CGAAGAAGATGTCATCACTT+GGG + chr5.1:28844288-28844307 MS.gene49594:CDS 40.0%
CTTAACACCAAGAGATGTTG+TGG + chr5.1:28844235-28844254 MS.gene49594:CDS 40.0%
GCCACCCATCGATAATTATA+TGG + chr5.1:28844345-28844364 MS.gene49594:CDS 40.0%
GCTGCAACCACATAATCATT+TGG - chr5.1:28844433-28844452 None:intergenic 40.0%
GGAATCTACAACGTAAAGCA+TGG + chr5.1:28845009-28845028 MS.gene49594:CDS 40.0%
TCTGAAGGGATCATTAGCAT+AGG - chr5.1:28844145-28844164 None:intergenic 40.0%
TGTTATTCAAGTGACCCTTG+GGG + chr5.1:28844613-28844632 MS.gene49594:CDS 40.0%
TTATGAAAGCTAGACAGCCA+CGG - chr5.1:28844468-28844487 None:intergenic 40.0%
! CACTTGGGAGAATCCTTTTA+AGG + chr5.1:28844303-28844322 MS.gene49594:CDS 40.0%
! CCTAGAGGTCCTTTTGATAT+GGG + chr5.1:28844988-28845007 MS.gene49594:CDS 40.0%
! TCCTAGAGGTCCTTTTGATA+TGG + chr5.1:28844987-28845006 MS.gene49594:CDS 40.0%
!! TCAAAAGGACCTCTAGGATA+AGG - chr5.1:28844985-28845004 None:intergenic 40.0%
AAAAGGTGGCACAACCCATA+TGG - chr5.1:28845088-28845107 None:intergenic 45.0%
AAAGGTGGCACAACCCATAT+GGG - chr5.1:28845087-28845106 None:intergenic 45.0%
AAGGTATGACACCTCCCATA+TGG + chr5.1:28845070-28845089 MS.gene49594:CDS 45.0%
AATCATTGGAGGGAGAGTTG+TGG + chr5.1:28844710-28844729 MS.gene49594:CDS 45.0%
ACTTGAGCCACAACATCTCT+TGG - chr5.1:28844245-28844264 None:intergenic 45.0%
AGATGTTGTGGCTCAAGTCA+TGG + chr5.1:28844247-28844266 MS.gene49594:CDS 45.0%
AGGTATGACACCTCCCATAT+GGG + chr5.1:28845071-28845090 MS.gene49594:CDS 45.0%
CCCATATCAAAAGGACCTCT+AGG - chr5.1:28844991-28845010 None:intergenic 45.0%
GAATTCTTTCCTCACCCCAA+GGG - chr5.1:28844630-28844649 None:intergenic 45.0%
TGGTGTATGCTGTAACTCGT+TGG + chr5.1:28844566-28844585 MS.gene49594:CDS 45.0%
! GAACCGATTCCTTATCCTAG+AGG + chr5.1:28844973-28844992 MS.gene49594:CDS 45.0%
!! GGACCTCTAGGATAAGGAAT+CGG - chr5.1:28844979-28844998 None:intergenic 45.0%
!! GGTGATCGTGTTGATGACTA+AGG - chr5.1:28844102-28844121 None:intergenic 45.0%
ATTGGAGGGAGAGTTGTGGA+TGG + chr5.1:28844714-28844733 MS.gene49594:CDS 50.0%
GAGAGTTGTGGATGGTGAGA+AGG + chr5.1:28844722-28844741 MS.gene49594:CDS 50.0%
GCAGCAATTTACACTGACCG+TGG + chr5.1:28844448-28844467 MS.gene49594:CDS 50.0%
GGAATTCTTTCCTCACCCCA+AGG - chr5.1:28844631-28844650 None:intergenic 50.0%
! GGGTTGTGCCACCTTTTCAA+TGG + chr5.1:28845091-28845110 MS.gene49594:CDS 50.0%
GTTGTGGCTCAAGTCATGGC+TGG + chr5.1:28844251-28844270 MS.gene49594:CDS 55.0%
TTCAAGTGACCCTTGGGGTG+AGG + chr5.1:28844618-28844637 MS.gene49594:CDS 55.0%
GGTGGCACAACCCATATGGG+AGG - chr5.1:28845084-28845103 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 28843977 28845119 28843977 ID=MS.gene49594
chr5.1 mRNA 28843977 28845119 28843977 ID=MS.gene49594.t1;Parent=MS.gene49594
chr5.1 exon 28843977 28845119 28843977 ID=MS.gene49594.t1.exon1;Parent=MS.gene49594.t1
chr5.1 CDS 28843977 28845119 28843977 ID=cds.MS.gene49594.t1;Parent=MS.gene49594.t1
Gene Sequence

>MS.gene49594

ATGGAGCGAGATTGGGCAAACTTAGATTCACTCGCTCTCAACATGATTCTTGAGAAGTTAATTGAACCAATTGATCACATTTGGTTTGGTTCTGTCTGCAAAAATTGGCACTCAGTTGCAGACCTTAGTCATCAACACGATCACCAATTTAGGAGCAATATAATGCCTATGCTAATGATCCCTTCAGAAAAGAGTCCAGAAAAGCGATGTTTGTATAGCATACCTGAAAATAGAGTGTATCCATTAGAATTGACAATGCTTAACACCAAGAGATGTTGTGGCTCAAGTCATGGCTGGCTCGCAACGTTAGACGAAGAAGATGTCATCACTTGGGAGAATCCTTTTAAGGATGTTGCTGCTATTAGTTTGCCACCCATCGATAATTATATGGTATGTAAGGATTACGACTACAATGTGCATAAGGTTACATTGTCGGTTGATCCTATAACAAGTCCAAATGATTATGTGGTTGCAGCAATTTACACTGACCGTGGCTGTCTAGCTTTCATAAAAGCAGGACAAGAGTTTTGGACATACATACAAGACACTGACCATTTTGGTTTCATCGACATTACATTCTACAATGGTTTGGTGTATGCTGTAACTCGTTGGAGAAAAATTGTCTGCTTTGATCTTTGTTATTCAAGTGACCCTTGGGGTGAGGAAAGAATTCCAAATGTTTTGTTACAAAGAAGCGATGATGCAACTTATTCTCCACTTACTTATTTAGTGAAATCATTGGAGGGAGAGTTGTGGATGGTGAGAAGGTTTATAAATAGGGTCAAAAATATCAACAAAGGTACTAATTCTTTACATGTATTTAAGTTGGAATTGAATGACAAAGGTGAGAAGCTTATGCATTTGTCGAAACTTGAGAGTTTAGGAGACAATATTTTATTTGTAGGTGACGGCGACTCAATGTCTGTATCAACTTCATATTTCTCAAGTTACCTCCAAAAGGATTCAATTTACTATTCTGATAATTATTATAACGAAGAACCGATTCCTTATCCTAGAGGTCCTTTTGATATGGGAATCTACAACGTAAAGCATGGAAGCTTTGATGTTCATTGCCGTTACAAATCTTACTTCAAAGGTATGACACCTCCCATATGGGTTGTGCCACCTTTTCAATGGAATTGA
Protein sequence

>MS.gene49594.t1

MERDWANLDSLALNMILEKLIEPIDHIWFGSVCKNWHSVADLSHQHDHQFRSNIMPMLMIPSEKSPEKRCLYSIPENRVYPLELTMLNTKRCCGSSHGWLATLDEEDVITWENPFKDVAAISLPPIDNYMVCKDYDYNVHKVTLSVDPITSPNDYVVAAIYTDRGCLAFIKAGQEFWTYIQDTDHFGFIDITFYNGLVYAVTRWRKIVCFDLCYSSDPWGEERIPNVLLQRSDDATYSPLTYLVKSLEGELWMVRRFINRVKNINKGTNSLHVFKLELNDKGEKLMHLSKLESLGDNILFVGDGDSMSVSTSYFSSYLQKDSIYYSDNYYNEEPIPYPRGPFDMGIYNVKHGSFDVHCRYKSYFKGMTPPIWVVPPFQWN