AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031136


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031136.t1 MTR_1g064570 74.597 248 58 3 33 278 156 400 1.09e-132 381
MS.gene031136.t1 MTR_5g038260 55.020 249 100 4 33 278 144 383 5.16e-90 272
MS.gene031136.t1 MTR_5g038230 48.980 245 91 5 33 276 142 353 6.35e-71 222
MS.gene031136.t1 MTR_5g082780 41.772 237 110 6 13 245 18 230 9.05e-50 164
MS.gene031136.t1 MTR_7g076100 39.441 322 126 11 2 278 3 300 9.37e-50 166
MS.gene031136.t1 MTR_1g108740 41.463 246 105 7 33 276 136 344 3.36e-43 150
MS.gene031136.t1 MTR_6g023955 92.105 76 6 0 33 108 77 152 1.57e-42 145
MS.gene031136.t1 MTR_6g023955 83.871 62 10 0 217 278 149 210 1.54e-32 119
MS.gene031136.t1 MTR_7g077660 40.684 263 101 10 33 278 144 368 1.20e-39 142
MS.gene031136.t1 MTR_7g077620 39.764 254 116 11 33 276 144 370 6.01e-38 137
MS.gene031136.t1 MTR_7g077650 38.290 269 104 12 33 278 143 372 1.39e-35 131
MS.gene031136.t1 MTR_7g076110 37.786 262 124 11 29 278 79 313 3.80e-35 129
MS.gene031136.t1 MTR_5g082770 47.059 170 75 4 33 202 136 290 2.44e-33 123
MS.gene031136.t1 MTR_7g076130 36.552 145 73 4 48 185 16 148 2.01e-16 75.1
MS.gene031136.t1 MTR_3g114990 27.434 226 142 9 29 250 173 380 2.13e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATGTCGGCTTCGTATTT+TGG 0.208690 6.2:+8814551 MS.gene031136:CDS
AATGTTAAAGCTTGATAGTT+TGG 0.240213 6.2:+8814490 MS.gene031136:CDS
TAAAAGCTGGACAAAAGTTT+TGG 0.255927 6.2:+8814117 MS.gene031136:CDS
TATGTTGTAGTTGCAATTTA+TGG 0.256703 6.2:+8814071 MS.gene031136:CDS
TGGCTTGTAAGGAAATTTAT+TGG 0.269879 6.2:+8814356 MS.gene031136:CDS
TTCATATCAAATGGTCCATT+AGG 0.289459 6.2:-8814641 None:intergenic
TCATATCAAATGGTCCATTA+GGG 0.347171 6.2:-8814640 None:intergenic
GTCAATGGAGGCAGTAGATT+GGG 0.349372 6.2:+8813584 None:intergenic
CGAAGTGTATAAGTTGGAAT+TGG 0.356208 6.2:+8814442 MS.gene031136:CDS
ATGTTAAAGCTTGATAGTTT+GGG 0.391733 6.2:+8814491 MS.gene031136:CDS
TCCTAATGTTGTTTCACCAT+GGG 0.397638 6.2:+8814280 MS.gene031136:CDS
AGGGAGACTTGTGGCTTGTA+AGG 0.407147 6.2:+8814345 MS.gene031136:CDS
AGCTATTATTCCCACTGAAA+TGG 0.434392 6.2:-8814755 None:intergenic
TGTCAATGGAGGCAGTAGAT+TGG 0.445882 6.2:+8813583 None:intergenic
TGTCTTGCTTTCATAAAAGC+TGG 0.450654 6.2:+8814104 MS.gene031136:CDS
AAAATCTATAATGTAAAAGA+TGG 0.455567 6.2:+8814662 MS.gene031136:CDS
TATCGGCAAATCCTACAATC+AGG 0.460348 6.2:+8814039 MS.gene031136:CDS
GTTCACACGGATGCCACCTT+CGG 0.463341 6.2:+8814718 MS.gene031136:CDS
AAGATTCGAAGTGTATAAGT+TGG 0.476468 6.2:+8814436 MS.gene031136:CDS
GCACCGGATCCTTACCCTAA+TGG 0.478062 6.2:+8814626 MS.gene031136:CDS
TAATCGTGAGGCCTGATTGT+AGG 0.479756 6.2:-8814050 None:intergenic
CCCCATGGTGAAACAACATT+AGG 0.481080 6.2:-8814281 None:intergenic
AGCATTGCAATTTCGAACAT+TGG 0.498979 6.2:+8814696 MS.gene031136:CDS
TCATCATCAGTGTCATCATC+GGG 0.504068 6.2:-8814383 None:intergenic
TTCATCATCAGTGTCATCAT+CGG 0.513248 6.2:-8814384 None:intergenic
GGTTCTTCCACCATTTCAGT+GGG 0.514018 6.2:+8814745 MS.gene031136:CDS
GGGTTCTTCCACCATTTCAG+TGG 0.530111 6.2:+8814744 MS.gene031136:CDS
TAATGTTGTTTCACCATGGG+GGG 0.533333 6.2:+8814283 MS.gene031136:CDS
CACGGATGCCACCTTCGGTG+TGG 0.534966 6.2:+8814723 MS.gene031136:CDS
TAGTCTATGCTGCGGGACGA+TGG 0.540068 6.2:+8814195 MS.gene031136:CDS
TCAAATGGTCCATTAGGGTA+AGG 0.543154 6.2:-8814635 None:intergenic
TTCCTAATGTTGTTTCACCA+TGG 0.544368 6.2:+8814279 MS.gene031136:CDS
ACGGATGCCACCTTCGGTGT+GGG 0.551054 6.2:+8814724 MS.gene031136:CDS
TGATTATTTCGAAGAAGCAC+CGG 0.565311 6.2:+8814610 MS.gene031136:CDS
GGTCCATTAGGGTAAGGATC+CGG 0.584997 6.2:-8814629 None:intergenic
GGAAGAACCCACACCGAAGG+TGG 0.585668 6.2:-8814731 None:intergenic
AAAGGGTTAGTCTATGCTGC+GGG 0.586027 6.2:+8814188 MS.gene031136:CDS
TAGTGATTCTGTATCTATGT+CGG 0.590343 6.2:+8814538 MS.gene031136:CDS
CCTAATGTTGTTTCACCATG+GGG 0.597547 6.2:+8814281 MS.gene031136:CDS
ATTTCGAACATTGGTTCACA+CGG 0.602276 6.2:+8814705 MS.gene031136:CDS
GATAGTAACTTGCCTAGTAG+TGG 0.603948 6.2:+8814407 MS.gene031136:CDS
TATTATTCCCACTGAAATGG+TGG 0.613749 6.2:-8814752 None:intergenic
TCAATGGAGGCAGTAGATTG+GGG 0.615269 6.2:+8813585 None:intergenic
TAAAGGGTTAGTCTATGCTG+CGG 0.625231 6.2:+8814187 MS.gene031136:CDS
AATCATTGGAGGGAGACTTG+TGG 0.640759 6.2:+8814336 MS.gene031136:CDS
GGTGGAAGAACCCACACCGA+AGG 0.641630 6.2:-8814734 None:intergenic
TTGGAATTGGATCTTCAAAG+TGG 0.643699 6.2:+8814455 MS.gene031136:CDS
CTAATGTTGTTTCACCATGG+GGG 0.657132 6.2:+8814282 MS.gene031136:CDS
CGATCACATTTATTTCAGCG+TGG 0.681214 6.2:+8813662 MS.gene031136:CDS
GCAACTACAACATAATCGTG+AGG 0.708433 6.2:-8814062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCTATAATGTAAAAGA+TGG + chr6.2:8814662-8814681 MS.gene031136:CDS 15.0%
!!! TTATAGATTTTCATATCAAA+TGG - chr6.2:8814653-8814672 None:intergenic 15.0%
!! TTGTTAAAAATCAAACTAAG+AGG - chr6.2:8813627-8813646 None:intergenic 20.0%
!!! AGCGATGTTATATTTTATAA+AGG + chr6.2:8814170-8814189 MS.gene031136:CDS 20.0%
!!! GCGATGTTATATTTTATAAA+GGG + chr6.2:8814171-8814190 MS.gene031136:CDS 20.0%
!!! GTAATACATTGTTTTTGATT+TGG - chr6.2:8813734-8813753 None:intergenic 20.0%
!!! TTTTGGTAATTATCTACAAA+AGG + chr6.2:8814568-8814587 MS.gene031136:CDS 20.0%
! AGAACAAAGAGATGTTTATA+TGG + chr6.2:8813786-8813805 MS.gene031136:intron 25.0%
! CTAACTCTTCTATATATATC+TGG - chr6.2:8813987-8814006 None:intergenic 25.0%
! GAACAAAGAGATGTTTATAT+GGG + chr6.2:8813787-8813806 MS.gene031136:intron 25.0%
! TAGGGTTATTATTGTAAGTT+TGG - chr6.2:8813913-8813932 None:intergenic 25.0%
! TATGTTGTAGTTGCAATTTA+TGG + chr6.2:8814071-8814090 MS.gene031136:CDS 25.0%
! TTATGAATGTAACGTTCATA+AGG + chr6.2:8814010-8814029 MS.gene031136:intron 25.0%
!! AATGTTAAAGCTTGATAGTT+TGG + chr6.2:8814490-8814509 MS.gene031136:CDS 25.0%
!! AGCATATTTTGTGAAATCAT+TGG + chr6.2:8814322-8814341 MS.gene031136:CDS 25.0%
!! ATATTTTGTGAAATCATTGG+AGG + chr6.2:8814325-8814344 MS.gene031136:CDS 25.0%
!! ATGGAAACTAGATTTTTGAA+GGG - chr6.2:8813942-8813961 None:intergenic 25.0%
!! ATGTTAAAGCTTGATAGTTT+GGG + chr6.2:8814491-8814510 MS.gene031136:CDS 25.0%
!! TATTTTGTGAAATCATTGGA+GGG + chr6.2:8814326-8814345 MS.gene031136:CDS 25.0%
!! TTGATAACTAAACTTTGCAA+TGG - chr6.2:8813706-8813725 None:intergenic 25.0%
!! TTTTGATATTTGTAACTCGA+AGG + chr6.2:8814232-8814251 MS.gene031136:CDS 25.0%
!!! ATTTTTGAAGGGATTTAGTA+GGG - chr6.2:8813931-8813950 None:intergenic 25.0%
!!! CGTTCATAAGGTTATTTTAT+CGG + chr6.2:8814022-8814041 MS.gene031136:intron 25.0%
AAGATTCGAAGTGTATAAGT+TGG + chr6.2:8814436-8814455 MS.gene031136:CDS 30.0%
ATATATATCTGGCATGTAGA+TGG - chr6.2:8813976-8813995 None:intergenic 30.0%
TAATGATTCCCACAAAAAGA+AGG + chr6.2:8813760-8813779 MS.gene031136:intron 30.0%
TAGTGATTCTGTATCTATGT+CGG + chr6.2:8814538-8814557 MS.gene031136:CDS 30.0%
TATATATCTGGCATGTAGAT+GGG - chr6.2:8813975-8813994 None:intergenic 30.0%
TCATATCAAATGGTCCATTA+GGG - chr6.2:8814643-8814662 None:intergenic 30.0%
TTCATATCAAATGGTCCATT+AGG - chr6.2:8814644-8814663 None:intergenic 30.0%
! AATCTTTTAGTACCACTACT+AGG - chr6.2:8814422-8814441 None:intergenic 30.0%
! GATGGAAACTAGATTTTTGA+AGG - chr6.2:8813943-8813962 None:intergenic 30.0%
! TAAAAGCTGGACAAAAGTTT+TGG + chr6.2:8814117-8814136 MS.gene031136:CDS 30.0%
! TGGCTTGTAAGGAAATTTAT+TGG + chr6.2:8814356-8814375 MS.gene031136:CDS 30.0%
! TTGGAAGTGGTAAATTTTGT+TGG - chr6.2:8813820-8813839 None:intergenic 30.0%
!! GATTTTTGAAGGGATTTAGT+AGG - chr6.2:8813932-8813951 None:intergenic 30.0%
!!! GGACACAATGTTTTATTTGT+GGG + chr6.2:8814512-8814531 MS.gene031136:CDS 30.0%
AGCATTGCAATTTCGAACAT+TGG + chr6.2:8814696-8814715 MS.gene031136:CDS 35.0%
TGTCTTGCTTTCATAAAAGC+TGG + chr6.2:8814104-8814123 MS.gene031136:CDS 35.0%
TGTTATAAGGGAGTGGTAAT+TGG - chr6.2:8813839-8813858 None:intergenic 35.0%
TTCATCATCAGTGTCATCAT+CGG - chr6.2:8814387-8814406 None:intergenic 35.0%
TTGGAATTGGATCTTCAAAG+TGG + chr6.2:8814455-8814474 MS.gene031136:CDS 35.0%
TTTGTGGGAATCATTAGCAT+AGG - chr6.2:8813756-8813775 None:intergenic 35.0%
! CACAAAGCCTCTTGTTATAA+GGG - chr6.2:8813851-8813870 None:intergenic 35.0%
! CGAAGTGTATAAGTTGGAAT+TGG + chr6.2:8814442-8814461 MS.gene031136:CDS 35.0%
! GTTCTACACCTTCTTTTTGT+GGG - chr6.2:8813771-8813790 None:intergenic 35.0%
! TGATTATTTCGAAGAAGCAC+CGG + chr6.2:8814610-8814629 MS.gene031136:CDS 35.0%
! TGTTCTACACCTTCTTTTTG+TGG - chr6.2:8813772-8813791 None:intergenic 35.0%
!! ATTTCGAACATTGGTTCACA+CGG + chr6.2:8814705-8814724 MS.gene031136:CDS 35.0%
!! TCCTAATGTTGTTTCACCAT+GGG + chr6.2:8814280-8814299 MS.gene031136:CDS 35.0%
!! TTCCTAATGTTGTTTCACCA+TGG + chr6.2:8814279-8814298 MS.gene031136:CDS 35.0%
!!! GGGACACAATGTTTTATTTG+TGG + chr6.2:8814511-8814530 MS.gene031136:CDS 35.0%
CCTTATAACAAGAGGCTTTG+TGG + chr6.2:8813849-8813868 MS.gene031136:intron 40.0%
CGATCACATTTATTTCAGCG+TGG + chr6.2:8813662-8813681 MS.gene031136:CDS 40.0%
GCAACTACAACATAATCGTG+AGG - chr6.2:8814065-8814084 None:intergenic 40.0%
TACCACTCCCTTATAACAAG+AGG + chr6.2:8813841-8813860 MS.gene031136:intron 40.0%
TATCGGCAAATCCTACAATC+AGG + chr6.2:8814039-8814058 MS.gene031136:CDS 40.0%
TCAAATGGTCCATTAGGGTA+AGG - chr6.2:8814638-8814657 None:intergenic 40.0%
TCATCATCAGTGTCATCATC+GGG - chr6.2:8814386-8814405 None:intergenic 40.0%
TTGTGGTTCAAGTCATGGTT+GGG + chr6.2:8813866-8813885 MS.gene031136:intron 40.0%
TTTGTGGTTCAAGTCATGGT+TGG + chr6.2:8813865-8813884 MS.gene031136:intron 40.0%
! CCACAAAGCCTCTTGTTATA+AGG - chr6.2:8813852-8813871 None:intergenic 40.0%
! GATAGTAACTTGCCTAGTAG+TGG + chr6.2:8814407-8814426 MS.gene031136:CDS 40.0%
!! CCTAATGTTGTTTCACCATG+GGG + chr6.2:8814281-8814300 MS.gene031136:CDS 40.0%
!! CTAATGTTGTTTCACCATGG+GGG + chr6.2:8814282-8814301 MS.gene031136:CDS 40.0%
!! TAAAGGGTTAGTCTATGCTG+CGG + chr6.2:8814187-8814206 MS.gene031136:CDS 40.0%
!! TAATGTTGTTTCACCATGGG+GGG + chr6.2:8814283-8814302 MS.gene031136:CDS 40.0%
!!! TCTATGTCGGCTTCGTATTT+TGG + chr6.2:8814551-8814570 MS.gene031136:CDS 40.0%
AAGGGAGTGGTAATTGGAAG+TGG - chr6.2:8813833-8813852 None:intergenic 45.0%
AAGTCATGGTTGGGTAGCAA+AGG + chr6.2:8813875-8813894 MS.gene031136:intron 45.0%
AATCATTGGAGGGAGACTTG+TGG + chr6.2:8814336-8814355 MS.gene031136:CDS 45.0%
ATATCTGGCATGTAGATGGG+TGG - chr6.2:8813972-8813991 None:intergenic 45.0%
CCCCATGGTGAAACAACATT+AGG - chr6.2:8814284-8814303 None:intergenic 45.0%
GCATGTAGATGGGTGGTAAA+GGG - chr6.2:8813965-8813984 None:intergenic 45.0%
GGTTCTTCCACCATTTCAGT+GGG + chr6.2:8814745-8814764 MS.gene031136:CDS 45.0%
TAATCGTGAGGCCTGATTGT+AGG - chr6.2:8814053-8814072 None:intergenic 45.0%
TCAGCGTGGTATGCAAGAAT+TGG + chr6.2:8813676-8813695 MS.gene031136:intron 45.0%
! AGCCTCTTGTTATAAGGGAG+TGG - chr6.2:8813846-8813865 None:intergenic 45.0%
! AGGCTTTGTGGTTCAAGTCA+TGG + chr6.2:8813861-8813880 MS.gene031136:intron 45.0%
!! AAAGGGTTAGTCTATGCTGC+GGG + chr6.2:8814188-8814207 MS.gene031136:CDS 45.0%
GGCATGTAGATGGGTGGTAA+AGG - chr6.2:8813966-8813985 None:intergenic 50.0%
GGGTTCTTCCACCATTTCAG+TGG + chr6.2:8814744-8814763 MS.gene031136:CDS 50.0%
GGTCCATTAGGGTAAGGATC+CGG - chr6.2:8814632-8814651 None:intergenic 50.0%
GTAGATGGGTGGTAAAGGGA+TGG - chr6.2:8813961-8813980 None:intergenic 50.0%
TCATGGTTGGGTAGCAAAGG+TGG + chr6.2:8813878-8813897 MS.gene031136:intron 50.0%
TGAGCAAAATCATCCCCCCA+TGG - chr6.2:8814299-8814318 None:intergenic 50.0%
! AGGGAGACTTGTGGCTTGTA+AGG + chr6.2:8814345-8814364 MS.gene031136:CDS 50.0%
GCACCGGATCCTTACCCTAA+TGG + chr6.2:8814626-8814645 MS.gene031136:CDS 55.0%
GTTCACACGGATGCCACCTT+CGG + chr6.2:8814718-8814737 MS.gene031136:CDS 55.0%
TAGTCTATGCTGCGGGACGA+TGG + chr6.2:8814195-8814214 MS.gene031136:CDS 55.0%
GGAAGAACCCACACCGAAGG+TGG - chr6.2:8814734-8814753 None:intergenic 60.0%
GGTGGAAGAACCCACACCGA+AGG - chr6.2:8814737-8814756 None:intergenic 60.0%
! ACGGATGCCACCTTCGGTGT+GGG + chr6.2:8814724-8814743 MS.gene031136:CDS 60.0%
CACGGATGCCACCTTCGGTG+TGG + chr6.2:8814723-8814742 MS.gene031136:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 8813588 8814772 8813588 ID=MS.gene031136
chr6.2 mRNA 8813588 8814772 8813588 ID=MS.gene031136.t1;Parent=MS.gene031136
chr6.2 exon 8813588 8813683 8813588 ID=MS.gene031136.t1.exon1;Parent=MS.gene031136.t1
chr6.2 CDS 8813588 8813683 8813588 ID=cds.MS.gene031136.t1;Parent=MS.gene031136.t1
chr6.2 exon 8814032 8814772 8814032 ID=MS.gene031136.t1.exon2;Parent=MS.gene031136.t1
chr6.2 CDS 8814032 8814772 8814032 ID=cds.MS.gene031136.t1;Parent=MS.gene031136.t1
Gene Sequence

>MS.gene031136

ATGGAGGCAGTAGATTGGGGAAACTTAGAAGCACTTCCTCTTAGTTTGATTTTTAACAAGTTAGTAGAGCGTATCGATCACATTTATTTCAGCGTGGTATGCAAGAATTGGTATTCCATTGCAAAGTTTAGTTATCAAAATCGCCAAATCAAAAACAATGTATTACCTATGCTAATGATTCCCACAAAAAGAAGGTGTAGAACAAAGAGATGTTTATATGGGATTTCATCCAACAAAATTTACCACTTCCAATTACCACTCCCTTATAACAAGAGGCTTTGTGGTTCAAGTCATGGTTGGGTAGCAAAGGTGGATTTTATTTCCAAACTTACAATAATAACCCTACTAAATCCCTTCAAAAATCTAGTTTCCATCCCTTTACCACCCATCTACATGCCAGATATATATAGAAGAGTTAGATATTATGAATGTAACGTTCATAAGGTTATTTTATCGGCAAATCCTACAATCAGGCCTCACGATTATGTTGTAGTTGCAATTTATGGTATGCGTAAATGTCTTGCTTTCATAAAAGCTGGACAAAAGTTTTGGACTTACGTTGATGACGACTATTTTTGCTTCAGCGATGTTATATTTTATAAAGGGTTAGTCTATGCTGCGGGACGATGGAATAACATTGTCTCTTTTGATATTTGTAACTCGAAGGATTCTTTTGATTGTGAGAAGATCATTCCTAATGTTGTTTCACCATGGGGGGATGATTTTGCTCATCGAGCATATTTTGTGAAATCATTGGAGGGAGACTTGTGGCTTGTAAGGAAATTTATTGGTTTTCCCGATGATGACACTGATGATGAAGATAGTAACTTGCCTAGTAGTGGTACTAAAAGATTCGAAGTGTATAAGTTGGAATTGGATCTTCAAAGTGGTAAGCTTATACAAATGTTAAAGCTTGATAGTTTGGGACACAATGTTTTATTTGTGGGTGATAGTGATTCTGTATCTATGTCGGCTTCGTATTTTGGTAATTATCTACAAAAGGATTCAATTTATTACACTGATGATTATTTCGAAGAAGCACCGGATCCTTACCCTAATGGACCATTTGATATGAAAATCTATAATGTAAAAGATGGAAGCTTTAGTAAGCATTGCAATTTCGAACATTGGTTCACACGGATGCCACCTTCGGTGTGGGTTCTTCCACCATTTCAGTGGGAATAA

Protein sequence

>MS.gene031136.t1

MEAVDWGNLEALPLSLIFNKLVERIDHIYFSVVILSANPTIRPHDYVVVAIYGMRKCLAFIKAGQKFWTYVDDDYFCFSDVIFYKGLVYAAGRWNNIVSFDICNSKDSFDCEKIIPNVVSPWGDDFAHRAYFVKSLEGDLWLVRKFIGFPDDDTDDEDSNLPSSGTKRFEVYKLELDLQSGKLIQMLKLDSLGHNVLFVGDSDSVSMSASYFGNYLQKDSIYYTDDYFEEAPDPYPNGPFDMKIYNVKDGSFSKHCNFEHWFTRMPPSVWVLPPFQWE