AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52163

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52163.t1 MTR_7g076110 93.930 313 19 0 58 370 1 313 0.0 582
MS.gene52163.t1 MTR_7g077620 55.673 379 148 8 1 368 1 370 8.87e-132 382
MS.gene52163.t1 MTR_7g077650 53.109 386 151 12 1 370 1 372 1.62e-120 353
MS.gene52163.t1 MTR_7g077660 52.660 376 157 9 5 370 4 368 2.78e-117 345
MS.gene52163.t1 MTR_7g076100 60.084 238 91 4 134 370 66 300 3.35e-91 276
MS.gene52163.t1 MTR_7g076100 83.333 54 9 0 1 54 1 54 2.07e-24 102
MS.gene52163.t1 MTR_5g038260 38.660 388 205 14 6 370 6 383 1.69e-72 231
MS.gene52163.t1 MTR_1g108740 42.359 373 178 14 3 368 2 344 2.34e-72 229
MS.gene52163.t1 MTR_5g082770 44.408 304 143 9 6 302 4 288 5.35e-72 226
MS.gene52163.t1 MTR_5g038230 36.176 387 186 13 6 368 4 353 2.17e-65 211
MS.gene52163.t1 MTR_1g064570 38.350 412 194 16 5 370 3 400 3.41e-65 212
MS.gene52163.t1 MTR_1g016150 45.550 191 74 7 3 175 2 180 4.81e-42 147
MS.gene52163.t1 MTR_7g076130 56.349 126 53 2 163 287 24 148 9.23e-39 136
MS.gene52163.t1 MTR_5g082780 37.778 225 110 8 109 325 6 208 3.47e-32 121
MS.gene52163.t1 MTR_1g108750 44.000 150 53 4 6 154 4 123 5.10e-25 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCAAACCAACCATTT+TGG 0.209572 7.4:-29374891 MS.gene52163:CDS
GACAGTGTTCCAGAAGATTT+AGG 0.219883 7.4:-29374409 MS.gene52163:CDS
TTCTCATAGTAGGTAAAATT+AGG 0.225887 7.4:+29374733 None:intergenic
ACCATCAAAACCATCAATTT+AGG 0.249142 7.4:-29375257 MS.gene52163:CDS
AGTACTCTTTGTGGCTTAAA+AGG 0.260035 7.4:+29375169 None:intergenic
ACCTGTCATGGCTCTTCATT+TGG 0.288626 7.4:-29375117 MS.gene52163:CDS
ATCCCAAACCCTAAATCTTC+TGG 0.300792 7.4:+29374400 None:intergenic
GGAATAATACCATACTTAAA+AGG 0.315387 7.4:+29375048 None:intergenic
CGACGTTTACAAATTGAAAC+TGG 0.332871 7.4:-29374578 MS.gene52163:CDS
GTTCCAGAAGATTTAGGGTT+TGG 0.341176 7.4:-29374403 MS.gene52163:CDS
AATGAACAGACTTAAGAGTT+TGG 0.348149 7.4:-29374530 MS.gene52163:CDS
AGAAGAACTTCCTCTTAGTT+TGG 0.350191 7.4:-29375361 MS.gene52163:CDS
TTCCAGAAGATTTAGGGTTT+GGG 0.356926 7.4:-29374402 MS.gene52163:CDS
GTCATGGCTCTTCATTTGGT+TGG 0.362670 7.4:-29375113 MS.gene52163:CDS
GTAACTATAGGAATCCAAAA+TGG 0.366794 7.4:+29374877 None:intergenic
TGTAATAAATTTGTAACTAT+AGG 0.375568 7.4:+29374865 None:intergenic
ACAGTGTTCCAGAAGATTTA+GGG 0.377757 7.4:-29374408 MS.gene52163:CDS
ACACTCCGGAGATATCTTAA+AGG 0.378694 7.4:+29374994 None:intergenic
AAGTTTGTCCAGCATAATCT+TGG 0.388756 7.4:+29374680 None:intergenic
AAAAGGATTGAGTAGAGTTA+TGG 0.390478 7.4:+29375065 None:intergenic
GGGCTCTACCTCAATTTCAA+TGG 0.397463 7.4:-29374306 MS.gene52163:CDS
CACATAGATCATCTTAGATT+CGG 0.408172 7.4:-29375318 MS.gene52163:CDS
ATGAACAGACTTAAGAGTTT+GGG 0.410853 7.4:-29374529 MS.gene52163:CDS
GGCTCTACCTCAATTTCAAT+GGG 0.416041 7.4:-29374305 MS.gene52163:CDS
GCTGTCGTCATTTACAACTA+TGG 0.418694 7.4:-29374928 MS.gene52163:CDS
TCTCATAGTAGGTAAAATTA+GGG 0.423599 7.4:+29374734 None:intergenic
GCTAGTTATCTTCTCATAGT+AGG 0.423741 7.4:+29374723 None:intergenic
TACTGCTCTTGGATTCGATT+GGG 0.430675 7.4:+29375205 None:intergenic
ATTGAATGGAGTACTCTTTG+TGG 0.430687 7.4:+29375160 None:intergenic
ACGACAGCAACATAATCATC+TGG 0.430984 7.4:+29374943 None:intergenic
TTAGGGTCATTTGAGCGATC+AGG 0.435005 7.4:+29374751 None:intergenic
GACATCGAAAAGCTTTCATA+TGG 0.441021 7.4:-29374604 MS.gene52163:CDS
TTCGAGAGGTGGCAGACAAA+TGG 0.442248 7.4:+29375023 None:intergenic
GTATAGTATTCCTAAATTGA+TGG 0.447518 7.4:+29375247 None:intergenic
CGATGAGGAAGGTGGTAGAC+TGG 0.455468 7.4:-29375392 MS.gene52163:CDS
TATCTTGTGAAATCATTGAA+TGG 0.459383 7.4:-29374658 MS.gene52163:CDS
GATTGGGACGCTCATGATCA+TGG 0.463327 7.4:+29375221 None:intergenic
AATCAATCCCATTGAAATTG+AGG 0.474667 7.4:+29374298 None:intergenic
TAATCATCTGGGCTTATGAT+GGG 0.479866 7.4:+29374955 None:intergenic
TCGAGAGGTGGCAGACAAAT+GGG 0.480481 7.4:+29375024 None:intergenic
ATACAAGGATCTACTGCTCT+TGG 0.483271 7.4:+29375194 None:intergenic
ATAATCATCTGGGCTTATGA+TGG 0.483695 7.4:+29374954 None:intergenic
CTACTGCTCTTGGATTCGAT+TGG 0.489995 7.4:+29375204 None:intergenic
TAGGGTCATTTGAGCGATCA+GGG 0.500420 7.4:+29374752 None:intergenic
AATCATTGAATGGAGACATA+TGG 0.507395 7.4:-29374648 MS.gene52163:CDS
AGTTTGTCCAGCATAATCTT+GGG 0.508944 7.4:+29374681 None:intergenic
TTGTCAAGAACCAAACTAAG+AGG 0.509205 7.4:+29375351 None:intergenic
TCGGTTTCGTGTGCAAGACT+TGG 0.511651 7.4:-29375299 MS.gene52163:CDS
AGGAGGAGTGTTGAACTTGA+AGG 0.513586 7.4:+29374777 None:intergenic
ACAAGAGGCAGACACAGAAA+AGG 0.515918 7.4:+29374471 None:intergenic
TGTGATGGATGAAAATTGAA+TGG 0.520449 7.4:+29375146 None:intergenic
GTGGCTTAAAAGGCTATACA+AGG 0.542292 7.4:+29375179 None:intergenic
CTATAGGAATCCAAAATGGT+TGG 0.542954 7.4:+29374881 None:intergenic
CACCACCTTTAAGATATCTC+CGG 0.544228 7.4:-29374999 MS.gene52163:CDS
GGGATCTATAATGTGAAGGA+TGG 0.545871 7.4:-29374382 MS.gene52163:CDS
ATTGGGACGCTCATGATCAT+GGG 0.549814 7.4:+29375222 None:intergenic
ACCAAATGAAGAGCCATGAC+AGG 0.552193 7.4:+29375116 None:intergenic
GCTAGAGCCCAAGATTATGC+TGG 0.553236 7.4:-29374688 MS.gene52163:CDS
TGCGTCTGCTCGAAGGAAAG+TGG 0.572553 7.4:+29374337 None:intergenic
ATCACATTTATAACCTGTCA+TGG 0.576549 7.4:-29375129 MS.gene52163:CDS
ATCTGGGCTTATGATGGGGT+CGG 0.585200 7.4:+29374960 None:intergenic
GATGAGGAAGGTGGTAGACT+GGG 0.589322 7.4:-29375391 MS.gene52163:CDS
CTTTCATTAGTCCACAGTAA+CGG 0.591490 7.4:-29375090 MS.gene52163:CDS
CGACAGCAACATAATCATCT+GGG 0.592571 7.4:+29374944 None:intergenic
ATGACAGGTTATAAATGTGA+TGG 0.594341 7.4:+29375131 None:intergenic
GGGTCATTTGAGCGATCAGG+GGG 0.608939 7.4:+29374754 None:intergenic
AAGTTTCTTAACGATGAGGA+AGG 0.609754 7.4:-29375403 None:intergenic
AAAGGTGGTGATTCGAGAGG+TGG 0.610801 7.4:+29375012 None:intergenic
TTTGAACTTGGAGTAACAAG+AGG 0.615336 7.4:+29374456 None:intergenic
GTTTGGGATCTATAATGTGA+AGG 0.626362 7.4:-29374386 MS.gene52163:CDS
TTTCTTAACGATGAGGAAGG+TGG 0.631528 7.4:-29375400 None:intergenic
AATCATCTGGGCTTATGATG+GGG 0.632274 7.4:+29374956 None:intergenic
AGGGTCATTTGAGCGATCAG+GGG 0.648257 7.4:+29374753 None:intergenic
CTCCGGAGATATCTTAAAGG+TGG 0.652726 7.4:+29374997 None:intergenic
CGGCAGACAATGTAACACTC+CGG 0.665324 7.4:+29374980 None:intergenic
GAGTTATGGAACCGTTACTG+TGG 0.666466 7.4:+29375079 None:intergenic
GGGACGCTCATGATCATGGG+TGG 0.671811 7.4:+29375225 None:intergenic
CTTAAAGGTGGTGATTCGAG+AGG 0.674686 7.4:+29375009 None:intergenic
ATTGAATGGAGACATATGGA+TGG 0.693585 7.4:-29374644 MS.gene52163:CDS
TTGAAACTGGACACCCAGAG+TGG 0.696035 7.4:-29374565 MS.gene52163:CDS
GGATTGAATAGTGTCTGAGT+GGG 0.704793 7.4:+29374798 None:intergenic
AGAGGTGGCAGACAAATGGG+AGG 0.713505 7.4:+29375027 None:intergenic
AGGATTGAATAGTGTCTGAG+TGG 0.717909 7.4:+29374797 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATAAATTTGTAACTAT+AGG + chr7.4:29374828-29374847 None:intergenic 15.0%
!!! AATGATGTTGTATTTTATAA+AGG - chr7.4:29374852-29374871 MS.gene52163:CDS 15.0%
!!! ATTTTATTTATCGGATATTG+TGG - chr7.4:29375191-29375210 MS.gene52163:CDS 20.0%
! GGAATAATACCATACTTAAA+AGG + chr7.4:29374645-29374664 None:intergenic 25.0%
! GTATAGTATTCCTAAATTGA+TGG + chr7.4:29374446-29374465 None:intergenic 25.0%
! TATCTTGTGAAATCATTGAA+TGG - chr7.4:29375032-29375051 MS.gene52163:CDS 25.0%
! TCTCATAGTAGGTAAAATTA+GGG + chr7.4:29374959-29374978 None:intergenic 25.0%
! TTCTCATAGTAGGTAAAATT+AGG + chr7.4:29374960-29374979 None:intergenic 25.0%
!!! AACTGAATCTTTTTTGAACT+TGG + chr7.4:29375249-29375268 None:intergenic 25.0%
!!! GGAGACAACATTTTATTTAT+CGG - chr7.4:29375182-29375201 MS.gene52163:CDS 25.0%
AAAAGGATTGAGTAGAGTTA+TGG + chr7.4:29374628-29374647 None:intergenic 30.0%
AATCATTGAATGGAGACATA+TGG - chr7.4:29375042-29375061 MS.gene52163:CDS 30.0%
AATGAACAGACTTAAGAGTT+TGG - chr7.4:29375160-29375179 MS.gene52163:CDS 30.0%
ACCATCAAAACCATCAATTT+AGG - chr7.4:29374433-29374452 MS.gene52163:CDS 30.0%
ATCACATTTATAACCTGTCA+TGG - chr7.4:29374561-29374580 MS.gene52163:CDS 30.0%
ATGAACAGACTTAAGAGTTT+GGG - chr7.4:29375161-29375180 MS.gene52163:CDS 30.0%
ATGACAGGTTATAAATGTGA+TGG + chr7.4:29374562-29374581 None:intergenic 30.0%
CACATAGATCATCTTAGATT+CGG - chr7.4:29374372-29374391 MS.gene52163:CDS 30.0%
GTAACTATAGGAATCCAAAA+TGG + chr7.4:29374816-29374835 None:intergenic 30.0%
! TTGCTCTTTTGAATGCAATA+CGG + chr7.4:29374788-29374807 None:intergenic 30.0%
!! CTACTCAATCCTTTTAAGTA+TGG - chr7.4:29374633-29374652 MS.gene52163:CDS 30.0%
!! TGTGATGGATGAAAATTGAA+TGG + chr7.4:29374547-29374566 None:intergenic 30.0%
!!! TCCTAAATTGATGGTTTTGA+TGG + chr7.4:29374437-29374456 None:intergenic 30.0%
AAGTTTGTCCAGCATAATCT+TGG + chr7.4:29375013-29375032 None:intergenic 35.0%
ACAGTGTTCCAGAAGATTTA+GGG - chr7.4:29375282-29375301 MS.gene52163:CDS 35.0%
AGAAGAACTTCCTCTTAGTT+TGG - chr7.4:29374329-29374348 MS.gene52163:CDS 35.0%
AGTACTCTTTGTGGCTTAAA+AGG + chr7.4:29374524-29374543 None:intergenic 35.0%
AGTTTGTCCAGCATAATCTT+GGG + chr7.4:29375012-29375031 None:intergenic 35.0%
ATAATCATCTGGGCTTATGA+TGG + chr7.4:29374739-29374758 None:intergenic 35.0%
ATTGAATGGAGACATATGGA+TGG - chr7.4:29375046-29375065 MS.gene52163:CDS 35.0%
CGACGTTTACAAATTGAAAC+TGG - chr7.4:29375112-29375131 MS.gene52163:CDS 35.0%
CTATAGGAATCCAAAATGGT+TGG + chr7.4:29374812-29374831 None:intergenic 35.0%
CTTTCATTAGTCCACAGTAA+CGG - chr7.4:29374600-29374619 MS.gene52163:CDS 35.0%
GACATCGAAAAGCTTTCATA+TGG - chr7.4:29375086-29375105 MS.gene52163:CDS 35.0%
GCTAGTTATCTTCTCATAGT+AGG + chr7.4:29374970-29374989 None:intergenic 35.0%
TAATCATCTGGGCTTATGAT+GGG + chr7.4:29374738-29374757 None:intergenic 35.0%
TTGTCAAGAACCAAACTAAG+AGG + chr7.4:29374342-29374361 None:intergenic 35.0%
TTTGAACTTGGAGTAACAAG+AGG + chr7.4:29375237-29375256 None:intergenic 35.0%
! AAAGAGCAAACCAACCATTT+TGG - chr7.4:29374799-29374818 MS.gene52163:CDS 35.0%
! ATTGAATGGAGTACTCTTTG+TGG + chr7.4:29374533-29374552 None:intergenic 35.0%
! GTTTGGGATCTATAATGTGA+AGG - chr7.4:29375304-29375323 MS.gene52163:CDS 35.0%
! TTCCAGAAGATTTAGGGTTT+GGG - chr7.4:29375288-29375307 MS.gene52163:CDS 35.0%
! TTGTACAAGTTTTCCACTCT+GGG + chr7.4:29375141-29375160 None:intergenic 35.0%
! TTTGTACAAGTTTTCCACTC+TGG + chr7.4:29375142-29375161 None:intergenic 35.0%
AAAAAATTGCGTCTGCTCGA+AGG + chr7.4:29375363-29375382 None:intergenic 40.0%
AATCATCTGGGCTTATGATG+GGG + chr7.4:29374737-29374756 None:intergenic 40.0%
ACACTCCGGAGATATCTTAA+AGG + chr7.4:29374699-29374718 None:intergenic 40.0%
ACGACAGCAACATAATCATC+TGG + chr7.4:29374750-29374769 None:intergenic 40.0%
AGGATTGAATAGTGTCTGAG+TGG + chr7.4:29374896-29374915 None:intergenic 40.0%
ATACAAGGATCTACTGCTCT+TGG + chr7.4:29374499-29374518 None:intergenic 40.0%
ATCCCAAACCCTAAATCTTC+TGG + chr7.4:29375293-29375312 None:intergenic 40.0%
CGACAGCAACATAATCATCT+GGG + chr7.4:29374749-29374768 None:intergenic 40.0%
GACAGTGTTCCAGAAGATTT+AGG - chr7.4:29375281-29375300 MS.gene52163:CDS 40.0%
GCTGTCGTCATTTACAACTA+TGG - chr7.4:29374762-29374781 MS.gene52163:CDS 40.0%
GGATTGAATAGTGTCTGAGT+GGG + chr7.4:29374895-29374914 None:intergenic 40.0%
GGCTCTACCTCAATTTCAAT+GGG - chr7.4:29375385-29375404 MS.gene52163:CDS 40.0%
GGGATCTATAATGTGAAGGA+TGG - chr7.4:29375308-29375327 MS.gene52163:CDS 40.0%
GTGGCTTAAAAGGCTATACA+AGG + chr7.4:29374514-29374533 None:intergenic 40.0%
TACTGCTCTTGGATTCGATT+GGG + chr7.4:29374488-29374507 None:intergenic 40.0%
! GTTCCAGAAGATTTAGGGTT+TGG - chr7.4:29375287-29375306 MS.gene52163:CDS 40.0%
!! CACCACCTTTAAGATATCTC+CGG - chr7.4:29374691-29374710 MS.gene52163:CDS 40.0%
!!! CGAGCAGACGCAATTTTTTT+GGG - chr7.4:29375364-29375383 MS.gene52163:CDS 40.0%
!!! TCGAGCAGACGCAATTTTTT+TGG - chr7.4:29375363-29375382 MS.gene52163:CDS 40.0%
ACAAGAGGCAGACACAGAAA+AGG + chr7.4:29375222-29375241 None:intergenic 45.0%
ACCAAATGAAGAGCCATGAC+AGG + chr7.4:29374577-29374596 None:intergenic 45.0%
ACCTGTCATGGCTCTTCATT+TGG - chr7.4:29374573-29374592 MS.gene52163:CDS 45.0%
ATTGGGACGCTCATGATCAT+GGG + chr7.4:29374471-29374490 None:intergenic 45.0%
CTACTGCTCTTGGATTCGAT+TGG + chr7.4:29374489-29374508 None:intergenic 45.0%
CTCCGGAGATATCTTAAAGG+TGG + chr7.4:29374696-29374715 None:intergenic 45.0%
CTTAAAGGTGGTGATTCGAG+AGG + chr7.4:29374684-29374703 None:intergenic 45.0%
GAGTTATGGAACCGTTACTG+TGG + chr7.4:29374614-29374633 None:intergenic 45.0%
GGGCTCTACCTCAATTTCAA+TGG - chr7.4:29375384-29375403 MS.gene52163:CDS 45.0%
GTCATGGCTCTTCATTTGGT+TGG - chr7.4:29374577-29374596 MS.gene52163:CDS 45.0%
TAGGGTCATTTGAGCGATCA+GGG + chr7.4:29374941-29374960 None:intergenic 45.0%
TTAGGGTCATTTGAGCGATC+AGG + chr7.4:29374942-29374961 None:intergenic 45.0%
!! AGGAGGAGTGTTGAACTTGA+AGG + chr7.4:29374916-29374935 None:intergenic 45.0%
AAAGGTGGTGATTCGAGAGG+TGG + chr7.4:29374681-29374700 None:intergenic 50.0%
AGGGTCATTTGAGCGATCAG+GGG + chr7.4:29374940-29374959 None:intergenic 50.0%
CGGCAGACAATGTAACACTC+CGG + chr7.4:29374713-29374732 None:intergenic 50.0%
GATTGGGACGCTCATGATCA+TGG + chr7.4:29374472-29374491 None:intergenic 50.0%
GCTAGAGCCCAAGATTATGC+TGG - chr7.4:29375002-29375021 MS.gene52163:CDS 50.0%
TCGAGAGGTGGCAGACAAAT+GGG + chr7.4:29374669-29374688 None:intergenic 50.0%
TCGGTTTCGTGTGCAAGACT+TGG - chr7.4:29374391-29374410 MS.gene52163:CDS 50.0%
TTCGAGAGGTGGCAGACAAA+TGG + chr7.4:29374670-29374689 None:intergenic 50.0%
TTGAAACTGGACACCCAGAG+TGG - chr7.4:29375125-29375144 MS.gene52163:CDS 50.0%
! ATCTGGGCTTATGATGGGGT+CGG + chr7.4:29374733-29374752 None:intergenic 50.0%
AGAGGTGGCAGACAAATGGG+AGG + chr7.4:29374666-29374685 None:intergenic 55.0%
GGGTCATTTGAGCGATCAGG+GGG + chr7.4:29374939-29374958 None:intergenic 55.0%
TCATTTGAGCGATCAGGGGG+AGG + chr7.4:29374936-29374955 None:intergenic 55.0%
TGCGTCTGCTCGAAGGAAAG+TGG + chr7.4:29375356-29375375 None:intergenic 55.0%
GGGACGCTCATGATCATGGG+TGG + chr7.4:29374468-29374487 None:intergenic 60.0%
TTTGAGCGATCAGGGGGAGG+AGG + chr7.4:29374933-29374952 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 29374300 29375412 29374300 ID=MS.gene52163
chr7.4 mRNA 29374300 29375412 29374300 ID=MS.gene52163.t1;Parent=MS.gene52163
chr7.4 exon 29374300 29375412 29374300 ID=MS.gene52163.t1.exon1;Parent=MS.gene52163.t1
chr7.4 CDS 29374300 29375412 29374300 ID=cds.MS.gene52163.t1;Parent=MS.gene52163.t1
Gene Sequence

>MS.gene52163

ATGAGGAAGGTGGTAGACTGGGCTAACTTAGAAGAACTTCCTCTTAGTTTGGTTCTTGACAAGTTAGAAGAACACATAGATCATCTTAGATTCGGTTTCGTGTGCAAGACTTGGCGATCAGTTGCAAAGCTTAACCATCAAAACCATCAATTTAGGAATACTATACCACCCATGATCATGAGCGTCCCAATCGAATCCAAGAGCAGTAGATCCTTGTATAGCCTTTTAAGCCACAAAGAGTACTCCATTCAATTTTCATCCATCACATTTATAACCTGTCATGGCTCTTCATTTGGTTGGCTTTCATTAGTCCACAGTAACGGTTCCATAACTCTACTCAATCCTTTTAAGTATGGTATTATTCCTCCCATTTGTCTGCCACCTCTCGAATCACCACCTTTAAGATATCTCCGGAGTGTTACATTGTCTGCCGACCCCATCATAAGCCCAGATGATTATGTTGCTGTCGTCATTTACAACTATGGCCGTATTGCATTCAAAAGAGCAAACCAACCATTTTGGATTCCTATAGTTACAAATTTATTACATTTCAATGATGTTGTATTTTATAAAGGCATGTTAGTCGCTGAGACCCACTCAGACACTATTCAATCCTTCAAGTTCAACACTCCTCCTCCCCCTGATCGCTCAAATGACCCTAATTTTACCTACTATGAGAAGATAACTAGCACTGAATATCGTGCTAGAGCCCAAGATTATGCTGGACAAACTTATCTTGTGAAATCATTGAATGGAGACATATGGATGGTGAAAAGATATCTAATCGACATCGAAAAGCTTTCATATGGACTCGACGTTTACAAATTGAAACTGGACACCCAGAGTGGAAAACTTGTACAAATGAACAGACTTAAGAGTTTGGGAGACAACATTTTATTTATCGGATATTGTGGTAATTCCTTTTCTGTGTCTGCCTCTTGTTACTCCAAGTTCAAAAAAGATTCAGTTTATTTTCTCTGTGACAGTGTTCCAGAAGATTTAGGGTTTGGGATCTATAATGTGAAGGATGGAAGCTATCAGATACAATCTTTACCACTTTCCTTCGAGCAGACGCAATTTTTTTGGGCTCTACCTCAATTTCAATGGGATTGA
Protein sequence

>MS.gene52163.t1

MRKVVDWANLEELPLSLVLDKLEEHIDHLRFGFVCKTWRSVAKLNHQNHQFRNTIPPMIMSVPIESKSSRSLYSLLSHKEYSIQFSSITFITCHGSSFGWLSLVHSNGSITLLNPFKYGIIPPICLPPLESPPLRYLRSVTLSADPIISPDDYVAVVIYNYGRIAFKRANQPFWIPIVTNLLHFNDVVFYKGMLVAETHSDTIQSFKFNTPPPPDRSNDPNFTYYEKITSTEYRARAQDYAGQTYLVKSLNGDIWMVKRYLIDIEKLSYGLDVYKLKLDTQSGKLVQMNRLKSLGDNILFIGYCGNSFSVSASCYSKFKKDSVYFLCDSVPEDLGFGIYNVKDGSYQIQSLPLSFEQTQFFWALPQFQWD