AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27750.t1 MTR_7g077620 68.280 372 114 3 1 370 1 370 3.32e-179 503
MS.gene27750.t1 MTR_7g077650 63.684 380 122 5 1 372 1 372 6.17e-161 456
MS.gene27750.t1 MTR_7g077660 62.304 382 120 5 1 372 1 368 3.48e-160 454
MS.gene27750.t1 MTR_7g076110 53.872 297 124 6 85 372 21 313 3.50e-97 291
MS.gene27750.t1 MTR_7g076100 61.765 238 85 4 138 372 66 300 8.28e-88 267
MS.gene27750.t1 MTR_7g076100 76.000 50 12 0 4 53 5 54 1.44e-19 88.6
MS.gene27750.t1 MTR_1g108740 43.386 378 172 15 1 370 1 344 1.09e-84 261
MS.gene27750.t1 MTR_5g038260 40.943 403 186 15 1 372 2 383 3.76e-77 243
MS.gene27750.t1 MTR_5g038230 39.547 397 162 16 5 370 4 353 7.84e-72 228
MS.gene27750.t1 MTR_1g064570 40.670 418 181 16 3 372 2 400 8.63e-71 227
MS.gene27750.t1 MTR_5g082770 43.631 314 131 9 5 302 4 287 4.61e-69 219
MS.gene27750.t1 MTR_1g016150 48.705 193 92 5 1 188 1 191 2.39e-48 164
MS.gene27750.t1 MTR_7g076130 63.710 124 42 1 168 288 25 148 5.98e-47 157
MS.gene27750.t1 MTR_5g082780 36.400 250 123 9 114 350 1 227 2.26e-33 124
MS.gene27750.t1 MTR_1g108750 47.134 157 45 5 5 159 4 124 7.51e-27 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAACTTCACGTTTACAAAT+TGG 0.193148 7.3:-29172181 MS.gene27750:CDS
TCAAGTGGATGCAATTGTTT+TGG 0.203842 7.3:-29171921 MS.gene27750:CDS
TGCATCCACTTGAAGGAAAT+TGG 0.206428 7.3:+29171931 None:intergenic
CGAGCTTTACATCTCTTAAA+AGG 0.209664 7.3:+29172618 None:intergenic
AAAGACCAAGCCAATCATTT+TGG 0.213196 7.3:-29172479 MS.gene27750:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7.3:+29172324 None:intergenic
ATGTGGTTGCAGCAATTTAC+AGG 0.290741 7.3:-29172521 MS.gene27750:CDS
ACCTCTCGAATCTCTCAAAA+AGG 0.312660 7.3:-29172583 MS.gene27750:CDS
AGCCTCTTGTTTCCCTGCAT+TGG 0.327895 7.3:-29172055 MS.gene27750:CDS
CCATGGTTGGCTTGCATTAG+TGG 0.328265 7.3:-29172670 MS.gene27750:CDS
GTATAAAACTAAAAGATGTT+TGG 0.337832 7.3:-29172700 MS.gene27750:CDS
GCTGATCAATGGAATGGGTT+AGG 0.348133 7.3:-29172003 MS.gene27750:CDS
AAGAACACATAGATCATGTT+AGG 0.348741 7.3:-29172926 MS.gene27750:CDS
AGAAGCATATCCTCTTAGTT+TGG 0.367450 7.3:-29172964 MS.gene27750:CDS
TATAAAACTAAAAGATGTTT+GGG 0.369727 7.3:-29172699 MS.gene27750:CDS
CAGCAATGACTAAGGTTGAT+TGG 0.374080 7.3:-29172995 None:intergenic
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7.3:+29172325 None:intergenic
AATTAACAGACTTAATAGTT+TGG 0.392756 7.3:-29172127 MS.gene27750:CDS
AAAAGGATTCACTAGAGTTA+TGG 0.411464 7.3:+29172635 None:intergenic
GTTGCAGCAATTTACAGGTT+TGG 0.412609 7.3:-29172516 MS.gene27750:CDS
GGGTTGTACCACAATTTCAA+TGG 0.418918 7.3:-29171900 MS.gene27750:CDS
CACATAGATCATGTTAGGTT+TGG 0.419733 7.3:-29172921 MS.gene27750:CDS
TTGTTTCCCTGCATTGGAAA+AGG 0.420899 7.3:-29172049 MS.gene27750:CDS
ATATGGAGGTGATTCACTTT+CGG 0.436618 7.3:-29172082 MS.gene27750:CDS
TCACGTTTACAAATTGGATC+TGG 0.437953 7.3:-29172175 MS.gene27750:CDS
GAATGAACCACCTATGCTAA+TGG 0.438887 7.3:-29172835 MS.gene27750:CDS
TAATTTCTAAAGCTGATCAA+TGG 0.449052 7.3:-29172014 MS.gene27750:CDS
CTTTACCAATTTCCTTCAAG+TGG 0.453302 7.3:-29171936 MS.gene27750:CDS
AAACAATTGCATCCACTTGA+AGG 0.458403 7.3:+29171924 None:intergenic
CTGATCAATGGAATGGGTTA+GGG 0.458755 7.3:-29172002 MS.gene27750:CDS
AGAGTAGAATAACATTGGTC+TGG 0.461740 7.3:+29172276 None:intergenic
TCTAAAGCTGATCAATGGAA+TGG 0.466690 7.3:-29172009 MS.gene27750:CDS
AGCAATGACTAAGGTTGATT+GGG 0.466704 7.3:-29172994 None:intergenic
ATGAGGATTGTTGAACTTGA+AGG 0.468030 7.3:+29172365 None:intergenic
TTACTTATCAGCAATGACTA+AGG 0.469229 7.3:-29173003 None:intergenic
GGTTGTACCACAATTTCAAT+GGG 0.477703 7.3:-29171899 MS.gene27750:CDS
CTACACGAATCCAAAATGAT+TGG 0.480728 7.3:+29172469 None:intergenic
CTAAAGCTGATCAATGGAAT+GGG 0.481933 7.3:-29172008 MS.gene27750:CDS
TAATCACTTGGATTTGTCAT+GGG 0.490766 7.3:+29172543 None:intergenic
TATTTCATGAAATCATTGAC+TGG 0.492434 7.3:-29172249 MS.gene27750:CDS
TTGTCAAGCACCAAACTAAG+AGG 0.503101 7.3:+29172954 None:intergenic
TAGTTTGGTGCTTGACAAGT+TGG 0.509534 7.3:-29172949 MS.gene27750:CDS
TAGGGTACTCTTTCTCACTT+AGG 0.509748 7.3:+29172745 None:intergenic
AATCATTGACTGGAGACATA+TGG 0.525440 7.3:-29172239 MS.gene27750:CDS
AGAATCGAAAATGTGAAGGA+TGG 0.527327 7.3:-29171976 MS.gene27750:CDS
ATAATCACTTGGATTTGTCA+TGG 0.537229 7.3:+29172542 None:intergenic
GTTTGGGTTGTAGCCATGGT+TGG 0.537462 7.3:-29172683 MS.gene27750:CDS
CGAATCCAAAATGATTGGCT+TGG 0.538749 7.3:+29172474 None:intergenic
GATCAATCCCATTGAAATTG+TGG 0.540140 7.3:+29171892 None:intergenic
TTGGTGTGGTGTGCAAGAGT+TGG 0.540593 7.3:-29172902 MS.gene27750:CDS
AGATGTTTGGGTTGTAGCCA+TGG 0.552870 7.3:-29172687 MS.gene27750:CDS
TGCGGCTATCAGCAAATACC+AGG 0.564512 7.3:+29172403 None:intergenic
GACAAATCCAAGTGATTATG+TGG 0.574598 7.3:-29172538 MS.gene27750:CDS
AAGAAAGAGTAGAATAACAT+TGG 0.575599 7.3:+29172271 None:intergenic
GAGAGGACCATTAGCATAGG+TGG 0.578665 7.3:+29172828 None:intergenic
GAGAAAATGTTTAATCGATG+AGG 0.590607 7.3:-29172211 MS.gene27750:CDS
GCTGCAACCACATAATCACT+TGG 0.591162 7.3:+29172531 None:intergenic
GTTAAGAATCGAAAATGTGA+AGG 0.593607 7.3:-29171980 MS.gene27750:CDS
GAAGGATGGGATAACCGACT+AGG 0.594551 7.3:+29172726 None:intergenic
AATCACTTGGATTTGTCATG+GGG 0.596697 7.3:+29172544 None:intergenic
GTTGAACTTGAAGGATACAA+TGG 0.598885 7.3:+29172374 None:intergenic
TACGAGACAATATGTTTGAG+AGG 0.606563 7.3:+29172811 None:intergenic
CCACTAATGCAAGCCAACCA+TGG 0.607842 7.3:+29172670 None:intergenic
TTTGAGAGGACCATTAGCAT+AGG 0.608355 7.3:+29172825 None:intergenic
TTCCAATGCAGGGAAACAAG+AGG 0.623982 7.3:+29172053 None:intergenic
AAGGATGGGATAACCGACTA+GGG 0.628975 7.3:+29172727 None:intergenic
TCATTTAAGTCATCACAATG+AGG 0.632337 7.3:+29172348 None:intergenic
GTAGACAAGCAAAATCAAAG+AGG 0.650836 7.3:-29172791 MS.gene27750:CDS
AGATCATGTTAGGTTTGGTG+TGG 0.661894 7.3:-29172916 MS.gene27750:CDS
GTGAGAAAGAGTACCCTAGT+CGG 0.682467 7.3:-29172740 MS.gene27750:CDS
TTGGATCTGGATGTGCAAAG+TGG 0.695381 7.3:-29172162 MS.gene27750:CDS
ATTGACTGGAGACATATGGA+TGG 0.700403 7.3:-29172235 MS.gene27750:CDS
AGGATACAATGGTATCTCTG+CGG 0.749692 7.3:+29172385 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTATTTATCAAATA+TGG - chr7.3:29172785-29172804 MS.gene27750:CDS 10.0%
!! ACCATAAAAATCATATATTT+AGG - chr7.3:29172024-29172043 MS.gene27750:CDS 15.0%
!! TATAAAACTAAAAGATGTTT+GGG - chr7.3:29172185-29172204 MS.gene27750:CDS 15.0%
!!! ATTTTATTTATCAAATATGG+AGG - chr7.3:29172788-29172807 MS.gene27750:CDS 15.0%
!!! TCCTAAATATATGATTTTTA+TGG + chr7.3:29172028-29172047 None:intergenic 15.0%
!! AATTAACAGACTTAATAGTT+TGG - chr7.3:29172757-29172776 MS.gene27750:CDS 20.0%
!! GTATAAAACTAAAAGATGTT+TGG - chr7.3:29172184-29172203 MS.gene27750:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG + chr7.3:29172562-29172581 None:intergenic 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG + chr7.3:29172563-29172582 None:intergenic 20.0%
!!! ACATCTTTTAGTTTTATACA+TGG + chr7.3:29172183-29172202 None:intergenic 20.0%
!!! ACTGATGTTGTATTTTATAA+AGG - chr7.3:29172458-29172477 MS.gene27750:CDS 20.0%
! AAGAAAGAGTAGAATAACAT+TGG + chr7.3:29172616-29172635 None:intergenic 25.0%
! TATTTCATGAAATCATTGAC+TGG - chr7.3:29172635-29172654 MS.gene27750:CDS 25.0%
!!! CTTTTAGTTTTATACATGGA+AGG + chr7.3:29172179-29172198 None:intergenic 25.0%
!!! TAATTTCTAAAGCTGATCAA+TGG - chr7.3:29172870-29172889 MS.gene27750:CDS 25.0%
AAAAGGATTCACTAGAGTTA+TGG + chr7.3:29172252-29172271 None:intergenic 30.0%
AAGAACACATAGATCATGTT+AGG - chr7.3:29171958-29171977 MS.gene27750:CDS 30.0%
ATAATCACTTGGATTTGTCA+TGG + chr7.3:29172345-29172364 None:intergenic 30.0%
GAGAAAATGTTTAATCGATG+AGG - chr7.3:29172673-29172692 MS.gene27750:CDS 30.0%
GTTAAGAATCGAAAATGTGA+AGG - chr7.3:29172904-29172923 MS.gene27750:CDS 30.0%
TAATCACTTGGATTTGTCAT+GGG + chr7.3:29172344-29172363 None:intergenic 30.0%
TCATTTAAGTCATCACAATG+AGG + chr7.3:29172539-29172558 None:intergenic 30.0%
! CATTGGTCTGGAAAAATATA+CGG + chr7.3:29172599-29172618 None:intergenic 30.0%
! TGTTGTATTTTATAAAGGCC+TGG - chr7.3:29172463-29172482 MS.gene27750:CDS 30.0%
!! AGTTTTATACATGGAAGGAT+GGG + chr7.3:29172174-29172193 None:intergenic 30.0%
!!! TAGTTTTATACATGGAAGGA+TGG + chr7.3:29172175-29172194 None:intergenic 30.0%
AAACAATTGCATCCACTTGA+AGG + chr7.3:29172963-29172982 None:intergenic 35.0%
AATCACTTGGATTTGTCATG+GGG + chr7.3:29172343-29172362 None:intergenic 35.0%
AGAATCGAAAATGTGAAGGA+TGG - chr7.3:29172908-29172927 MS.gene27750:CDS 35.0%
AGAGTAGAATAACATTGGTC+TGG + chr7.3:29172611-29172630 None:intergenic 35.0%
ATATGGAGGTGATTCACTTT+CGG - chr7.3:29172802-29172821 MS.gene27750:CDS 35.0%
ATGAGGATTGTTGAACTTGA+AGG + chr7.3:29172522-29172541 None:intergenic 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.3:29171963-29171982 MS.gene27750:CDS 35.0%
CGAACTTCACGTTTACAAAT+TGG - chr7.3:29172703-29172722 MS.gene27750:CDS 35.0%
CGAGCTTTACATCTCTTAAA+AGG + chr7.3:29172269-29172288 None:intergenic 35.0%
CTAAAGCTGATCAATGGAAT+GGG - chr7.3:29172876-29172895 MS.gene27750:CDS 35.0%
CTACACGAATCCAAAATGAT+TGG + chr7.3:29172418-29172437 None:intergenic 35.0%
CTTTACCAATTTCCTTCAAG+TGG - chr7.3:29172948-29172967 MS.gene27750:CDS 35.0%
GACAAATCCAAGTGATTATG+TGG - chr7.3:29172346-29172365 MS.gene27750:CDS 35.0%
GGTTGTACCACAATTTCAAT+GGG - chr7.3:29172985-29173004 MS.gene27750:CDS 35.0%
GTAGACAAGCAAAATCAAAG+AGG - chr7.3:29172093-29172112 MS.gene27750:CDS 35.0%
GTTGAACTTGAAGGATACAA+TGG + chr7.3:29172513-29172532 None:intergenic 35.0%
TACGAGACAATATGTTTGAG+AGG + chr7.3:29172076-29172095 None:intergenic 35.0%
TCACGTTTACAAATTGGATC+TGG - chr7.3:29172709-29172728 MS.gene27750:CDS 35.0%
! AAAGACCAAGCCAATCATTT+TGG - chr7.3:29172405-29172424 MS.gene27750:CDS 35.0%
! AATCATTGACTGGAGACATA+TGG - chr7.3:29172645-29172664 MS.gene27750:CDS 35.0%
! AGAAGCATATCCTCTTAGTT+TGG - chr7.3:29171920-29171939 MS.gene27750:CDS 35.0%
! CAAGTGGATGCAATTGTTTT+GGG - chr7.3:29172964-29172983 MS.gene27750:CDS 35.0%
! TCAAGTGGATGCAATTGTTT+TGG - chr7.3:29172963-29172982 MS.gene27750:CDS 35.0%
! TCTAAAGCTGATCAATGGAA+TGG - chr7.3:29172875-29172894 MS.gene27750:CDS 35.0%
ACCTCTCGAATCTCTCAAAA+AGG - chr7.3:29172301-29172320 MS.gene27750:CDS 40.0%
AGGATACAATGGTATCTCTG+CGG + chr7.3:29172502-29172521 None:intergenic 40.0%
ATGTGGTTGCAGCAATTTAC+AGG - chr7.3:29172363-29172382 MS.gene27750:CDS 40.0%
ATTGACTGGAGACATATGGA+TGG - chr7.3:29172649-29172668 MS.gene27750:CDS 40.0%
GAATGAACCACCTATGCTAA+TGG - chr7.3:29172049-29172068 MS.gene27750:CDS 40.0%
GGGTTGTACCACAATTTCAA+TGG - chr7.3:29172984-29173003 MS.gene27750:CDS 40.0%
GTTGCAGCAATTTACAGGTT+TGG - chr7.3:29172368-29172387 MS.gene27750:CDS 40.0%
TGCATCCACTTGAAGGAAAT+TGG + chr7.3:29172956-29172975 None:intergenic 40.0%
TTGTCAAGCACCAAACTAAG+AGG + chr7.3:29171933-29171952 None:intergenic 40.0%
TTGTTTCCCTGCATTGGAAA+AGG - chr7.3:29172835-29172854 MS.gene27750:CDS 40.0%
TTTGAGAGGACCATTAGCAT+AGG + chr7.3:29172062-29172081 None:intergenic 40.0%
! ATCGAATCCTTTTCCAATGC+AGG + chr7.3:29172845-29172864 None:intergenic 40.0%
! CGAATCCAAAATGATTGGCT+TGG + chr7.3:29172413-29172432 None:intergenic 40.0%
! TAGGGTACTCTTTCTCACTT+AGG + chr7.3:29172142-29172161 None:intergenic 40.0%
! TCGAATCCTTTTCCAATGCA+GGG + chr7.3:29172844-29172863 None:intergenic 40.0%
!! ACCTTTTTGAGAGATTCGAG+AGG + chr7.3:29172305-29172324 None:intergenic 40.0%
!! AGATCATGTTAGGTTTGGTG+TGG - chr7.3:29171968-29171987 MS.gene27750:CDS 40.0%
!! CTGATCAATGGAATGGGTTA+GGG - chr7.3:29172882-29172901 MS.gene27750:CDS 40.0%
!! TAGTTTGGTGCTTGACAAGT+TGG - chr7.3:29171935-29171954 MS.gene27750:CDS 40.0%
!! TTTTTGAGAGATTCGAGAGG+TGG + chr7.3:29172302-29172321 None:intergenic 40.0%
AAGGATGGGATAACCGACTA+GGG + chr7.3:29172160-29172179 None:intergenic 45.0%
GCTGCAACCACATAATCACT+TGG + chr7.3:29172356-29172375 None:intergenic 45.0%
TTCCAATGCAGGGAAACAAG+AGG + chr7.3:29172834-29172853 None:intergenic 45.0%
TTGGATCTGGATGTGCAAAG+TGG - chr7.3:29172722-29172741 MS.gene27750:CDS 45.0%
! GCTGATCAATGGAATGGGTT+AGG - chr7.3:29172881-29172900 MS.gene27750:CDS 45.0%
!! AGATGTTTGGGTTGTAGCCA+TGG - chr7.3:29172197-29172216 MS.gene27750:CDS 45.0%
!! GTGAGAAAGAGTACCCTAGT+CGG - chr7.3:29172144-29172163 MS.gene27750:CDS 45.0%
CCACTAATGCAAGCCAACCA+TGG + chr7.3:29172217-29172236 None:intergenic 50.0%
GAAGGATGGGATAACCGACT+AGG + chr7.3:29172161-29172180 None:intergenic 50.0%
GAGAGGACCATTAGCATAGG+TGG + chr7.3:29172059-29172078 None:intergenic 50.0%
TGCGGCTATCAGCAAATACC+AGG + chr7.3:29172484-29172503 None:intergenic 50.0%
TTGGTGTGGTGTGCAAGAGT+TGG - chr7.3:29171982-29172001 MS.gene27750:CDS 50.0%
! AGCCTCTTGTTTCCCTGCAT+TGG - chr7.3:29172829-29172848 MS.gene27750:CDS 50.0%
!! CCATGGTTGGCTTGCATTAG+TGG - chr7.3:29172214-29172233 MS.gene27750:CDS 50.0%
!! GTTTGGGTTGTAGCCATGGT+TGG - chr7.3:29172201-29172220 MS.gene27750:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 29171894 29173012 29171894 ID=MS.gene27750
chr7.3 mRNA 29171894 29173012 29171894 ID=MS.gene27750.t1;Parent=MS.gene27750
chr7.3 exon 29171894 29173012 29171894 ID=MS.gene27750.t1.exon1;Parent=MS.gene27750.t1
chr7.3 CDS 29171894 29173012 29171894 ID=cds.MS.gene27750.t1;Parent=MS.gene27750.t1
Gene Sequence

>MS.gene27750

ATGACTAAGGTTGATTGGGCAAATTTAGAAGCATATCCTCTTAGTTTGGTGCTTGACAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGTGTGGTGTGCAAGAGTTGGATATCAATTGCAAAGCTTAACCATAAAAATCATATATTTAGGATGAATGAACCACCTATGCTAATGGTCCTCTCAAACATATTGTCTCGTAGACAAGCAAAATCAAAGAGGAATGAGAGATTGTTATATAGTATCCTAAGTGAGAAAGAGTACCCTAGTCGGTTATCCCATCCTTCCATGTATAAAACTAAAAGATGTTTGGGTTGTAGCCATGGTTGGCTTGCATTAGTGGATAAAAACAATGCCATAACTCTAGTGAATCCTTTTAAGAGATGTAAAGCTCGCATTAGTCTTCCACCTCTCGAATCTCTCAAAAAGGTTACTTTGTCTGCTGACCCCATGACAAATCCAAGTGATTATGTGGTTGCAGCAATTTACAGGTTTGGTTATCTCGCTTTCAAAAGACCAAGCCAATCATTTTGGATTCGTGTAGACAGAAATAGATTTTATTTCACTGATGTTGTATTTTATAAAGGCCTGGTATTTGCTGATAGCCGCAGAGATACCATTGTATCCTTCAAGTTCAACAATCCTCATTGTGATGACTTAAATGACCCTAATTTTACTTACTATGAGAAGATAGCTAGTACTCCGTATATTTTTCCAGACCAATGTTATTCTACTCTTTCTTATTTCATGAAATCATTGACTGGAGACATATGGATGGTGAGAAAATGTTTAATCGATGAGGATTCATTCGAACTTCACGTTTACAAATTGGATCTGGATGTGCAAAGTGGAAGACTTGAACAAATTAACAGACTTAATAGTTTGGAAGACAACATTTTATTTATCAAATATGGAGGTGATTCACTTTCGGTGTCAGCCTCTTGTTTCCCTGCATTGGAAAAGGATTCGATTTATTTAATTTCTAAAGCTGATCAATGGAATGGGTTAGGGTTAAGAATCGAAAATGTGAAGGATGGAAGTTTTCACATTCGATCTTTACCAATTTCCTTCAAGTGGATGCAATTGTTTTGGGTTGTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene27750.t1

MTKVDWANLEAYPLSLVLDKLEEHIDHVRFGVVCKSWISIAKLNHKNHIFRMNEPPMLMVLSNILSRRQAKSKRNERLLYSILSEKEYPSRLSHPSMYKTKRCLGCSHGWLALVDKNNAITLVNPFKRCKARISLPPLESLKKVTLSADPMTNPSDYVVAAIYRFGYLAFKRPSQSFWIRVDRNRFYFTDVVFYKGLVFADSRRDTIVSFKFNNPHCDDLNDPNFTYYEKIASTPYIFPDQCYSTLSYFMKSLTGDIWMVRKCLIDEDSFELHVYKLDLDVQSGRLEQINRLNSLEDNILFIKYGGDSLSVSASCFPALEKDSIYLISKADQWNGLGLRIENVKDGSFHIRSLPISFKWMQLFWVVPQFQWD